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Early Developmental Exposure To Δ9-Tetrahydrocannabinol Causes Latent Behavioral, Growth, And Bioenergetic Effects In Danio Rerio, Astra Hahm

Honors Theses

Early developmental exposure to Δ9-tetrahydrocannabinol causes latent behavioral, growth, and bioenergetic effects in Danio rerio

(Under the direction of Dr. Kristine Willett)

Δ9-tetrahydrocannabinol (Δ9-THC) is used during pregnancy to mitigate symptoms such as nausea, pain, and insomnia. It is established that Δ9-THC exposure early in life causes adverse effects in children’s development including behavioral disorders. Our goal was to determine the latent and generational impacts on growth, behavior, and mitochondrial bioenergetics following exposure to Δ9-THC. Wildtype (5D) zebrafish were exposed to 0 (0.05% DMSO), 0.08, 0.4, or 1 µM Δ9-THC from 6-96 hours post-fertilization (hpf), then subsequently raised in clean …

The Effects Of Gamma Glutamyl Cysteinyl Ethyl Ester On Oxidative Stress Biomarkers In Traumatically Injured Rats, Jenna Shoemaker

Honors Theses

Primary injury is the original point where the impact force affects axonal tissue. Secondary injury is often the main complication in patients suffering from traumatic brain injury (TBI). Consequences of secondary injuries include oxidative stress, DNA damage, and protein oxidation, which can impact overall brain function (Moppett). Gamma-glutamyl cysteinyl ethyl ester (GCEE), a glutathione mimetic, has been used as an antioxidant to reduce oxidative stress. This study aims to determine if GCEE is neuroprotective against moderate TBI at extended time points. Results from this work will provide insight for determining the best extended time point to administer treatment. This study …

Anxious, Stressed, Or Overwhelmed: The Typical Sensory Processing Pattern Of First-Year Occupational Therapy Students, Caroline V. Holsclaw

Honors Theses

This research discusses the four sensory processing patterns, sensory sensitivity, sensory avoidant, sensory seeking, and low registration, demonstrated by first year occupational therapy doctoral program students. These four patterns describe how our brains process sensory information from the environment and from our bodies. These patterns have been linked to many things in one’s life such as their attachment styles, anxiety disorders, and quality of life, and neurologically connected to the parasympathetic nervous system which is involved with the fight or flight response. Understanding your own sensory processing pattern and preferences can help you understand how you can find sensory input …

Fixing Kentucky: A Comparative Analysis Of The Spay And Neuter Options Available To Rural Kentucky Counties, Emma M. Spurlock

Honors Theses

The objective of this research is to determine if there is a substantial difference in the availability of spay and neuter resources in rural Kentucky counties compared to urban Kentucky counties. An analysis of the Kentucky Animal Control Advisory board meeting minutes over the past ten years was conducted to determine how spay and neuter programs are funded, what counties receive funding, and the process for requesting spay and neuter grant support. Rural Kentucky communities are underserved regarding spay and neuter resources because of insufficient funding, limited veterinary and animal care, and inadequate government involvement. Improving access to spay and …

Actuarial Prediction Of Adhd Using Neuropsychological Data, Kimberlyn Williams

Honors Theses

Attention-Deficit/Hyperactivity Disorder (ADHD) is the most common psychiatric diagnosis in children and a frequent psychiatric diagnosis in adults. ADHD is a clinical diagnosis based on self-reported symptoms which makes accurate diagnosis challenging. Actuarial prediction has been demonstrated to be more accurate than clinical judgment. The current study explored an actuarial approach to predicting ADHD based on a comprehensive battery of neuropsychological tests. This project utilized test data from Meyers Neuropsychological Battery (MNB) to develop a logistic regression model to accurately predict diagnosed ADHD cases (n=65) from normal functioning cases (n=79) with a history of Mild Traumatic Brain Injury (mTBI). Binary …

Lmpact Of Salmonella Enterica Effector Proteins On Plant Physiology And Immunity, Sara Qader

Honors Theses

Salmonella enterica serovar Typhimurium are primarily recognized as pathogens associated with foodborne illnesses in humans. Salmonella also can colonize plant tissue effectively. The pathogen relies on type III secretion systems (T3SS) to infect mammals and plants successfully and counteract the immune response. Understanding the molecular mechanisms underlying Salmonella’s interactions with plants is essential for developing effective strategies to reduce its impact on human health and agriculture. Effector proteins, known for their role in modulating host-pathogen interactions, have been implicated in the virulence and pathogenicity of Salmonella. However, their specific functions and contributions to Salmonella’s interactions with plants remain largely unknown. …

The Relationship Between Sun Protective Behavior, Health Beliefs, Attitudes, And Norms Of Sun Exposure Among College Athletes, Avery B. Snyder

Honors Theses

The purpose of this study was to access the relationship between sun protective behavior, health beliefs, attitudes, and norms of sun exposure among college athletes. An electronic survey was provided to student athletes for about two weeks in October of 2023. Prior to student participation the Institutional Review Board approved the protocol and data collection. The survey was sent to the students by the athletic director through an app called Teamworks that connects to all the athletes at Coastal Carolina. The majority of students who took the survey reported they did not wear sunscreen in past games/practices. Of those who …

Synthesis And Characterization Of Ox-Nad Derivatives, Juanita Monteiro-Pai

Honors Theses

Energy metabolism is complex, and NAD, or nicotinamide adenine dinucleotide, plays a huge role as an organic redox cofactor, driving metabolic reactions. The abundance of pathways dependent on NAD has led to an increase in the studies of NAD and its precursors. As a result NR, or nicotinamide riboside, is now a popular pharmaceutical taken to increase levels of NAD in the body. NR is not only a precursor to NAD, but a metabolite that is found in extracellular regions such as urine and blood. While this molecule should be an intracellular molecule, the fact that it is found in …

Impacts Of Hurricane Idalia’S Surge On Coastal Sand Biogeochemistry, Wendy "Cor" Mchone

Honors Theses

Beaches are heavily influenced by extreme events, such as hurricanes. Biological and chemical processes, such as primary production and diagenesis are often interrupted by these events. To examine the effects of hurricanes on coastal biogeochemistry, this study used sand and porewater samples from Waties Island, SC, which were collected before and after Hurricane Idalia. The samples were analyzed for macronutrient concentrations, organic content, and chlorophyll concentrations. Macronutrient pore water concentration changes were not uniform. The inventory of nitrite decreased significantly after the storm, which was reflected in slight increases in the inventories of nitrate and ammonium. Concentrations of nitrate, which …

The Role Of Inflammatory Cells In The Sex Dimorphism Of Pulmonary Arterial Hypertension, Erin M. Schreck

Honors Theses

Pulmonary Arterial Hypertension (PAH) is a disease associated with high blood pressure specifically in the lung circulation. Symptoms include dyspnea and cyanosis, and the mean survival post-diagnosis is 2.8 years without treatment. PAH can be characterized by accumulating vascular damage which results in lesion formation causing a decrease in the diameter of vessels, obstruction of flow, and ultimately increased pressure on the right heart leading to failure. These lesions define an arteriopathy that is a hallmark of PAH which is, in part, dependent on infiltration of inflammatory cells into the vascular wall. PAH presents uniquely in males and females with …

Interspecific Venom Variation Among Three Shades Of Aphonopelma Tarantulas: A. Gabeli, A. Hentzi, And A. Anax, Jorjia Elmore

Honors Theses

Phenotypic variation is a common occurrence throughout the animal kingdom and can occur both interspecifically and intraspecifically. Interspecific variation evolves in response to differential selection between species whereas intraspecific variation is commonly expressed as sex-based or population-based differences. Both interspecific and intraspecific phenotypic differences occur frequently in venomous species including arachnids, and can often result in differences in venom yield, potency, and protein. The North American tarantulas (Theraphosidae: Aphonopelma), include approximately 35 species and little is known about their venom. To test for interspecific and intraspecific venom variation, we collected individuals from three species and generated proteomic profiles and Illumina …

Fiddler Crab Gut Microbiomes As An Indicator For Eutrophication Due To Waste-Water Contamination In Estuaries, Madison L. Geraci

Honors Theses

Estuaries are unique biogeochemical zones in coastal systems that provide a myriad of ecological functions: providing habitat for critically important fishery species, diverse species of plants and animals, and contributing to nutrient cycling. Estuaries are vulnerable to anthropogenic stressors as they are intersections between marine and terrestrial communities and are often the hotspot of coastal development and activity. Estuarine sediments act as repositories for anthropogenic contaminants, including excess nutrient runoffs and waste-water pollution that can cause states of eutrophication. Microorganisms are sensitive to these environmental changes, presenting a unique opportunity of being useful bioindicators of environmental stress. Isolating microorganisms from …

Measurement Of Transmission Efficiency Of Blue Light Blocking Devices, Jada Lee

Honors Theses

Technology and light sources have experienced a revolution in recent years leading to the production of light emitting diode (LED) bulbs. White-light LED bulbs undergo degradation over time, leading to a rise in color temperature and a proportional increase in the emission of blue light from these bulbs. The small size of LEDs makes them the optimal choice for electronic devices because of their limited screen size. This means that blue light now exists in red, green, and blue solid-state illumination systems that did not exist a decade ago. It is debated if blue light induces toxic effects on the …

From Cells To Clinics- The Role Of College Biology Education In Shaping Future Physician Assistants, Monica Manley

Honors Theses

This thesis provides a personal reflection on translating concepts and techniques learned in multiple biology courses (i.e. microbiology, neuroscience, comparative physiology, and genetics) in to clinical practice as an intern in the emergency room of a hospital. Each selected concept contains a description followed by an example of how this knowledge relates to a specified duty of a physician assistant with in an emergency medical setting.

Cell Type-Specific Production Of Amyloid Precursor Protein During Infection Is Dependent On The Strain Of Herpes Simplex Virus 1, Mia Elias

Honors Theses

Cleavage of amyloid precursor protein (APP) generates the antimicrobial peptide amyloid beta (Aβ), the accumulation of which is linked to Alzheimer’s Disease (AD) pathogenesis. Whether processing of APP contributes to anti-viral responses is debated. Herpes Simplex Virus Type 1 (HSV-1), a neurotropic double-stranded deoxyribonucleic acid (DNA) virus, establishes lifelong latency and can cause a range of outcomes, from asymptomatic infection to herpetic encephalitis (HSE). Whether disparate outcomes are dependent on HSV-1 strain is unknown. Using three differentially virulent HSV-1 strains, we compared the regulation of APP-associated proteolytic processing in both a corneal infection murine model and an in vitro model. …

Effects Of Age And Gender On Perceptions On Victims Of Financial Exploitation, Kathleen Hillman

Honors Theses

A report by the Center for Victim Research shows that younger adults are more often victims of general and identity fraud (Irvin-Erickson & Ricks, 2019, p. 9); however, research conducted on financial exploitation focuses heavily on older adults. However, a recent study by Gunderson and colleagues (2021) found that older and younger adults are highly susceptible to financial exploitation. The purpose of the current study is to determine if perceptions of vulnerability to financial exploitation vary by the age of the victim. A sample of undergraduate participants will be recruited from the University of Tennessee at Chattanooga through an online …

Screening Full-Sib And Half-Sib Families Of Chestnut Seedlings For Resistance To Cryphonectria Parasitica Using A Small Stem Assay And A Leaf-Disc Assay, Uma Plambeck

Honors Theses

Cryphonectria parasitica is the ascomycete fungus species that causes chestnut blight disease. Symptoms of chestnut blight include bark cankers and eventually the death of American chestnut, Castanea dentata. However, not all Castanea species are equally affected by the disease, and some Asian species appear highly resistant. The OALDS and SSA in this study aim to assist in screening for resistance to C. parasitica within a year. We investigated the effect of C. parasitica on three North American species, one European species, four East Asian species of Castanea, and nine full-sib families of TACF backcross hybrids. Our study used both a …

Effects Of Attractiveness And Relationship Type Of Perceptions Of Adolescent Sexual Grooming, Alexandra Shappley

Honors Theses

Child sexual grooming is a prevalent issue within the United States, especially with the rise of the internet and social media (Shakeshaft, 2004; Finkelhor et al., 2000; Winters et al., 2017). There has been less research done on adolescent sexual grooming; however, researchers have found that perceptions of adolescent sexual grooming vary depending on the gender and age of the offender and victim (Russel & Gyrus, 2022; Sahl & Keene, 2012). Researchers have found that people perceive a sexual grooming relationship as inflicting more harm if the perpetrator is a man (Hernandez et al., 2019), particularly an older man (Russel …

Microfaunal Remains From The Ryan-Harley Site, Savanna Caylor

Honors Theses

The Ryan Harley site is located in the Wacissa River basin in northern Florida. It is one of the few sites in the southeastern United States to contain a Suwannee point artifact. Several excavations in 2015 and 2017 showed extinct and extant faunal remains within and around the Suwannee component. The geoarchaeological data showed that whether these remains were in situ or not is inconclusive. This paper will focus on the microfloral and faunal remains to draw conclusions about the environment encountered during the Younger Dryas (12,900-11,700 cal B.P). The relationship between floral and faunal species present will help to …

Imagined Intergroup Contact As A Mechanism To Change Beliefs About Gender, Samantha Oyler

Honors Theses

Gordon Allport first proposed the Intergroup Contact Theory in 1954. According to his theory, contact between ingroup and outgroup members, under certain conditions, would lead to positive effects, specifically the reduction of prejudice. Since then, this theory has been expanded to include members of other majority/minority groups. Research suggests intergroup contact has positive effects for reduction of anti-trans prejudice. To date, the focus has largely been on the mechanisms driving changes in attitudes and intentions towards trans individuals through intergroup contact interventions. It is unclear whether this intervention might also promote the acquisition of more nuanced sociocultural understanding about gender, …

Investigating Thin Filament Assembly In C. Elegans Muscles, Angela Russ

Honors Theses

All striated muscles are composed of small units called sarcomeres [1]. Within each sarcomere, there are thin filaments composed of actin and thick filaments composed of myosin. The thin filaments extend from either end of the thick filament towards the middle of the sarcomere. The lengths of thin filaments are correlated to muscle contraction and force production [1], but it is currently unknown what regulates their lengths. Prior hypotheses based on vertebrate models have focused on a nebulin ruler model [2]. However, nebulin is not present within invertebrate muscles, and this has led to a major gap in the field’s …

Civil Disobedience Of Social Workers In Hospice, Jamie Leigh Roper

Honors Theses

No abstract provided.

Female Choice: Attraction In The Animal Kingdom, Lindsay Culpepper

Honors Theses

Across taxa, choosing a mate is one of the most crucial decisions a female can make. This choice can come with direct benefits like protection and parenting assistance, but it may also provide indirect benefits through the survival of her offspring and thus the continuation of her genes. The female considers numerous qualities when making that choice, evaluating aspects of potential mates’ physical appearance and behavior. Understanding female choice is crucial to effective animal conservation and management, as it has significant implications for captive breeding programs, both in terms of output and in preparing offspring for release. This thesis will …

Formation Of Diazirine Functionality For Propellant Applications, Abby Mock

Honors Theses

Our research focuses on a cost-effective approach which minimizes synthetic overhead when assembling compounds with diazirine functionality. The goals are to prevent the surplus use of ammonia in the formation of diaziridine functionality and use an IKA Electrosyn 2.0 in the oxidation of diaziridine functionality. Success with either specific aim will significantly reduce the synthetic overhead currently observed. Diazirine functionality plays a vital role in a vast number of applications. Two of interest to us include diazirine functionality as a next generation propellant and as a photoaffinity label. With the latter, this strategy plays a vital role in drug discovery …

Evolution Of Medical Ethics During The American Civil War, Luke Love

Honors Theses

This thesis will explore the relationship between medical ethics and the American Civil War through a microhistorical approach. This includes analyzing the ways in which the physician-patient relationship was conducted and which medical practices were deemed ethical and by whom. Changes that occurred in medical ethics during the war will be analyzed and explained. v

Thamnophis And Their Toxic Relationship: Testing For Intraspecific Venom Variation In Common Garter Snakes, Thamnophis Sirtalis, Grace E. Coppinger

Honors Theses

Venomous species, particularly snakes, have captured human interest for decades. Over the last few decades, scientists have studied front-fanged venomous snakes to develop drugs, treat human disease, better manage snakebites, and answer ecological and evolutionary questions. However, these studies have largely ignored rear-fanged snakes which account for two-thirds of total snake species, despite only accounting for 23 % of published venom gland transcriptomes. To address the lack of knowledge about rear-fanged snake (RFS) venom, we sequenced the messenger RNA from the Duvernoy’s gland in 9 male and 10 female Common Garter Snakes, Thamnophis sirtalis. I produced the first Duvernoy’s venom …

Dna Methylation Of The Agtri Promoter In A Hypertensive Population Of Kenyans, Michael M. Roque

Honors Theses

Cardiovascular disease (CVD), the leading cause of death worldwide, is rapidly increasing in low-and middle-income countries (LMICs). particularly those of Sub Saharan Africa (SSA). Hypertension is the leading risk factor for CVD and is a multifactorial disease with no single genetic cause. Increasingly, evidence indicates that hypertension is predisposed by environmental regulation of genes through heritable, yet modifiable, epigenetic changes to DNA leading to changes in gene expression. e.g. methylation. While understanding the etiology of hypertension in LMICs is a global priority, few epigenetic studies exist from populations living in SSA. The renin arigiotensin system (RAS) is the primary hormonal …

Development And Validation Of A Noninvasive, Portable, And Low-Cost Device To Detect Peripheral Artery Disease, Julia Louise Nelson

Honors Theses

Peripheral Artery Disease (PAD) is a progressive cardiovascular condition characterized by atherosclerosis in the extremities. It affects up to 10 million adults in the United States and is associated with an elevated risk of heart attack and stroke. Up to 50% of people with PAD are asymptomatic and undiagnosed. This presents the need for a rapid, inexpensive, and noninvasive screening tool that can be easily used to diagnose PAD. We developed a device, REFLO (Rapid Electromagnetic FLOw device), to detect low blood flow and diagnose PAD. The device uses lowpower radio frequency energy (35 GHz) to heat the skin and …

Interspecific Venom Variation Among Three Microsympatric Scorpion Species Facilitates Niche Partitioning, Diplocentrus Lindo, Vaejovis Intermedius, Centruroides Vittatus, Anne Elizabeth Thaxton

Honors Theses

Scorpions are globally distributed and very well known for their use of venom for prey capture and defense. Despite their abundance and high number of species, very little is known about the venom used by scorpions, and how the components of venom work to maximize the success of the individual. The venom of a species is directly produced by the venom gland, which can be found at the very end of the tail in the telson. To further our understanding of scorpion venom, we generated the first venom gland transcriptomes for three species from three different families, Centruroides vittatus, Diplocentrus …

Survey Of The Bolctes From Fish River Nature Preserve, Noah T. Nelson

Honors Theses

Boletes are a special kind of mushroom inside of the Kingdom Fungi that are distinguished from other mushrooms by their uniquely pored hymenium, as opposed to gills. Many boletes are ectomycorrhizal, meaning they are mutualists with vascular plants, and thus beneficial to the overall health of the terrestrial ecosystem. Some are well-known for their edibility and are of economic importance. In the United States 59 genus-level clades comprising 290 operational taxonomic units have been reported. The southeastern US and Gulf Coast regions however are less researched and recorded for their mushroom ecology. In Alabama, only 56 bolete species have been …