Digital Commons Network^™

Mobile Application For Biosensor Colorimetric Analysis, Eui Bin You

Honors Theses

Inexpensive paper-based biosensors can be valuable screening tools to test for various illnesses, but it is often challenging to design them to produce a visual change that can easily be identified by untrained users. This research examines one method of compensating for the lack of distinct visual cues by developing and testing a mobile application that uses a machine learning algorithm (k-Nearest Neighbors) to analyze a picture of a sensor and determine whether it shows a positive or negative result. The machine learning algorithm was trained on a set of labeled sensor images and k-fold cross-validation was used to analyze …

The Effect Of Degrading The Transcription Factor Nf-Kb Subunit Proteins On Nf-Kb' S Oncological Activity, Seongmin Lee

Honors Theses

Nuclear factor-kappa B (NF-κB) is a transcription factor that becomes functional when any two of its five component proteins (p50, p52, p65, c-Rel, and RelB) join together. NF-κB plays an important role in bringing out cell proliferation, or cell growth. When NF-κB malfunctions and becomes hyperactive, excessive NF-κB activity promotes abnormally high cell growth, which is a symptom of cancer. Because of its tie to cancer, NF-κB is commonly subjected to modification to curb cancer growth. In this project, each component protein of NF- κB was degraded via a method called RNAi to see if it would have any negative …

Analysis Of Mammalian Carboxypeptidase O Expression Patterns, Christian Bardan

Honors Theses

Carboxypeptidase 0 (CPO) is a protease that cuts acidic amino acids from the carboxy termini of substrate proteins. This study aims to describe the expression patterns of CPO in small mammals. We have sampled tissue from four different species: thirteen-lined ground squirrel, eastern chipmunk, eastern mole, and northern short-tailed shrew. Samples were analyzed via Western blot using two CPO antibodies. Immunoreactive bands likely to be CPO, with molecular weights approximately 42 kDa, were seen predominantly in kidney, liver, and intestinal tissue samples. This suggests CPO could play a specific physiological function in these organs.

Injury-Induced Neuronal Turnover With Zinc Sulfate Affects Cilitated Olfactory Sensory Neurons More Than Microvillous Olfactory Sensory Neurons In The Adult Zebrafish, James Hentig

Honors Theses

Zinc sulfate is a known olfactory toxicant, although its effects on the olfactory epithelium of zebrafish have not been examined. Previous observations utilizing Triton X-100 found selective retention of some olfactory sensory neurons and loss of others. Based on those findings, we hypothesized that the retention of the ability to sense amino acids, detected by microvillous olfactory sensory neurons, and loss of the ability to perceive bile salts, detected by ciliated olfactory sensory neurons, is a universal response to chemical ablation. Fish were treated with zinc sulfate and survived for 2, 5, 10 and 14 days post treatment and processed …

Missing The Trees For The Forest: The Socioecological Significance Of Dispersed Farmland Trees In Northern Ethiopia, Jacob A. Wall, Travis W. Reynolds

Honors Theses

Scattered trees are prominent features in the agricultural landscape of the Ethiopian highlands. The dry Afromontane forests of the Amhara Region in northern Ethiopia have faced centuries of deforestation - the FAO estimates only 3% of the region is forested today. The remaining landscape has been largely converted into agricultural and grazing lands, with the exception of some limited government-protected lands, as well as thousands of small forest fragments left around Orthodox Churches (“church forests”). But while a growing body of scholarship has highlighted the ecological and cultural importance of church forests and other natural forest fragments, the roles of …

Missing The Trees For The Forest: The Socioecological Significance Of Dispersed Farmland Trees In Northern Ethiopia, Jacob A. Wall

Honors Theses

Scattered trees are prominent features in the agricultural landscape of the Ethiopian highlands. The dry Afromontane forests of the Amhara Region in northern Ethiopia have faced centuries of deforestation - the FAO estimates only 3% of the region is forested today. The remaining landscape has been largely converted into agricultural and grazing lands, with the exception of some limited government-protected lands, as well as thousands of small forest fragments left around Orthodox Churches (“church forests”). But while a growing body of scholarship has highlighted the ecological and cultural importance of church forests and other natural forest fragments, the roles of …

Health Care Agency: Statewide Awareness Of Patient-Centered Care In Maine’S Mental Health Care Facilities, Holly Hogan A

Honors Theses

This research project focuses on patient-centered care (PCC) in the context of inpatient acute psychiatric units. Hospitals have been claiming their renewed outlooks on health-care via PCC. It has become an increasingly popular term to use on hospital websites and in presidential statements. The technologies and practices surrounding psychiatric care have evolved in such a way that patients are more welcomed to have an input in their care. This project discusses the discourse around PCC at Mental Health Care Facilities in Maine. There is no consensus on the definition; the meanings ascribed to it are derived from the individual institutions. …

Pharmaceutical And Personal Care Products Concentrations In The Belgrade Lakes: A Possible Threat To Aquatic Ecosystems And Human Health, Serena Haver

Honors Theses

Pharmaceuticals and active ingredients from personal care products (PPCPs) include prescription and over-the-counter drugs, sunscreen, fragrances, and soaps. Recently researchers have detected PPCPs in surface waters, posing a threat to aquatic biota and human health. These compounds can enter the environment through dumping, direct excretion, or sewage effluent streams. We tested 14 water samples from East Pond, Great Pond, and Long Pond in Maine for the presence of 23 different PPCPs. Of those, we only detected significant levels of caffeine, 1,7-dimethylxanthine, and amphetamine all below 1µg/L. There were no significant differences between any of the three lakes for any of …

Assessing Lakesmart: The Development And Effectiveness Of A Lake Protection Program, Alexa A. E. Junker

Honors Theses

Maine’s nearly 6,000 lakes are a vital resource for the state, generating $6 billion in annual economic activity and sustaining 52,000 jobs. Over the course of the last several decades, this resource has increasingly been threatened by development and related problems, especially nutrient runoff. LakeSmart is a lake protection program designed to stem the flow of nutrient runoff by promoting and rewarding the use lake-friendly landscaping practices.

For this project, I traced the history of LakeSmart from its roots in the Maine Department of Environmental Protection and, through stakeholder interviews and surveys, chronicled its development into the flagship program of …

Are The Brain And The Mind One? Neuromarketing And How Consumers Make Decisions, Emily Glaenzer

Honors Theses

Neuromarketing utilizes brain-imaging technology, such as electroencephalography (EEG) and functional magnetic resonance imaging (fMRI) machines, to understand consumers’ neurological responses to marketing stimuli. In this paper, I demonstrate how neuromarketing connects to the history of subliminal messaging and our current neuro-obsessed culture (neuroculture). These factors affect how critics view neuromarketing and the implications involved for the future of this study. I hypothesize that, when used correctly, neuromarketing can initiate a new section of the marketing world that will serve as a useful component to more traditional marketing practices. By taking away social bias and inaccurate answers present in market research, …

The Effect Of Temperature On The Supply And Demand For The American Lobster (Homarus Americanus) In The State Of Maine, Gaby E. Carpenter

Honors Theses

The Gulf of Maine (GOM) American lobster (Homarus americanus) stock accounts for 90% of the U.S. American lobster landings. This makes it an extremely valuable and important fishery on a national scale, but also to the state of Maine. In the past decade, the fishery has experienced rapid fluctuation in landings and price due to anthropogenic influences of the water temperature in the GOM. Given the economic importance of the fishery, it is important to understand the future impacts of climate change on the availability of lobster and economic consequences of these shifts. A bio-economic model is used in the …

Using Genetic Algorithms To Evolve Artificial Neural Networks, William T. Kearney

Honors Theses

This paper demonstrates that neuroevolution is an effective method to determine an optimal neural network topology. I provide an overview of the NeuroEvolution of Augmenting Topologies (NEAT) algorithm, and describe how unique characteristics of this algorithm solve various problem inherent to neuroevolution (namely the competing conventions problem and the challenges associated with protecting topological innovation). Parallelization is shown to greatly speed up efficiency, further reinforcing neuroevolution as a potential alternative to traditional backpropagation. I also demonstrate that appropriate parameter selection is critical in order to efficiently converge to an optimal topology. Lastly, I produce an example solution to a medical …

Development Of A Chemical Genetic Screen To Determine Synergistic Compounds With Laromustine In Treating Glioblastoma Multiforme Cultured Cells, Ryan Weeks

Honors Theses

Laromustine is a chemotherapeutic sulfonylhydrazine prodrug used in clinical trials to treat acute myeloid leukemia (AML) and glioblastoma multiforme (GBM). While treatment of AML with laromustine has more demonstrative clinical success, there are enough promising data against GBM to pursue additional pre-clinical and clinical experiments. To determine the synergistic effects caused by treating cultured GBM cells with laromustine and a library of FDA-approved compounds, a chemical genetic screen was developed. To optimize the screen, optimal cultured GBM cell seed density, growth period and maximum well capacity were determined. The treatment period for a lethal dose of laromustine in cultured GBM …

Experimental And Computational Study Of Methylphenylvinylidene And Its Rearrangements, Xi Yang

Honors Theses

In this study, the synthesis and properties were explored of the compound methylphenylvinylidene (MPV), a vinylidene carbene with methyl and phenyl groups as substituents. A phenanthrene-based precursor, 1-(1-phenylethylidene)-1a,9b-dihydro-1H-cyclopropa[l]phenanthrene, was synthesized in three steps and was subjected to photolysis in C₆D₆ at ambient temperature. During photolysis, the MPV carbene rearranged into 1-phenylpropyne via a 1,2-phenyl shift, instead of 1,2-methyl shift, confirmed by photolysis of ¹³C-labeled precursor. This experimental result is consistent with computational results using the CCSD(T)/cc-pVTZ//B3LYP/6-31+G* method. The calculations suggest that the 1,2-phenyl shift in singlet carbene requires 3.8 kcal/mol to overcome …

The Effect Of Cyanobacterium Gloeotrichia Echinulata In The Belgrade Lakes, Maine, Harriet T. Rothschild

Honors Theses

Gloeotrichia echinulata is a cyanobacteria species that has been increasingly forming blooms in oligotrophic lakes in the Northeastern United States. The Belgrade Lakes in central Maine have experienced increasing blooms over the past decades. Long Pond and Great Pond in the Belgrade Lakes region are popular locations for summer tourism and year-round residents. Research into G. echinulata is important to the Belgrade community because of potential effects to water quality, public health, and recreation. Studying G. echinulata bloom density throughout the summer and how it may affect the phosphorous cycle, the nitrogen cycle, and the plankton community will help scientists …

Spatial And Temporal Patterns In Irruptive Fringillid Movements, Paul J. Dougherty

Honors Theses

Historic Christmas Bird Count and eBird data indicate that during winter invasions, North American irruptive fringillid species occur in relatively even numbers across latitudes east of the Rocky Mountains. Most boreal breeding species show large southward invasions every other winter, with this pattern typically being strongest across the northern and mid United States. eBird data confirms that many northern fringillids irrupt synchronously, and both datasets also show positive correlations between the winter movements of southern breeding species in many areas. Winter abundance records show negative correlations between northern breeding species and southern breeding species in nearly all monitored areas of …

Environmental Contingency Of Seed-Fungi Interactions In Coexisting Invasive Purple Loosestrife And Native Cattail, Shayla R. Williams

Honors Theses

Purple loosestrife (Lythrum salicaria) is a highly invasive species able to quickly take over entire wetlands, especially after disturbances. Bountiful seed production and a persistent and prolific seed bank play a key role in loosestrife’s ability to invade. However, some competing native species, such as cattails (Typha spp.) have comparable seed production rates but less abundant seed banks, suggesting that there may be a difference in belowground seed survival. I investigated the abundance of loosestrife and cattail seeds in soils at roadside sites relative to above-ground stem densities. Given the importance of fungal pathogens to seed …

The Impact Of Endocrine-Disrupting Chemicals On Wildlife Conservation, Eda Reed

Honors Theses

Endocrine-disrupting chemicals (EDCs), such as those from plastics and pesticides, have been hypothesized to affect wildlife populations. According to the U.S. National Institute of Environmental Health Sciences, EDCs ‘are chemicals that may interfere with the body’s endocrine system and produce adverse developmental, reproductive, neurological, and immune effects in both humans and wildlife.’ A growing number of studies suggest wildlife are being exposed to EDCs, but how EDCs affect wildlife health and wildlife conservation is less well-understood. Through scientific literature analysis and a survey of wildlife conservationists and practitioners, this study aims to determine the current state of knowledge and data …

Ecological Predictors And Consequences Of Non-Native Earthworms In Kennebec County, Maine, Julia Rogers

Honors Theses

Non-native earthworms are found throughout much of the United States and southern Canada in areas that were glaciated during the most recent glaciation. These earthworms are changing nutrient cycling, modifying soil structure, and influencing diversity in forests throughout the Northern United States, and their invasion northward is facilitated by human activities. Although worms are present in compost piles and gardens of all Maine counties, there are no comprehensive studies of earthworms distributions in forested areas of Maine. I documented the presence and absence of earthworms in forested recreation areas in Kennebec County, Maine, and investigated ecological and landscape level variables …

The Effects Of Oral Probiotic Supplements On The Human Gut Microbiome, Erin Hudnall

Honors Theses

A diverse bacterial community makes up the human gut microbiome and is essential not only to digestion, but also to other physiological processes. This bacterial community is subject to change due to external factors including, but not limited to, age, diet, and lifestyle. This study observed the effects of oral probiotic supplements on this bacterial community. Fecal samples were collected from a single subject once each week during alternating two- to six-week cycles of taking and abstaining from the probiotic supplement. The samples were purified, the bacterial cells within lysed, and the enclosed DNA was collected. A portion of the …

Design And Synthesis Of Dual Opioids/Npff Ligands As Novel Analgesics With Reduced Tolerance Potential, John N. Yi

Honors Theses

Opioid use has become prevalent in today's culture. As a treatment for acute and chronic pain, opioids permeate in the public sphere as a seemingly easy solution. However, problems that come from the increased use of opiates, especially in the last fifteen years, have shown detrimental effects in society. Not only are all opioid-based analgesics considered addictive, but the nature of such painkillers also enables a phenomenon known as tolerance, where the body adapts to the use of exogenous opioids. This creates a situation where a more potent dose of painkiller is required for the same analgesic effect. The focus …

Vaccination Situation: A History Of Vaccine Refusal In The United States And Vaccine Beliefs At The University Of Mississippi, Austin Vitale

Honors Theses

The goals of this research were to trace a narrative of vaccine refusal in the United States from the nation's inception to the present day and identify any impact or influence from refusal ideology on vaccine beliefs of University of Mississippi undergraduates. A review of historical literature regarding vaccine refusal in the United States developed a historical narrative, and a quantitative survey was utilized to identify the vaccine beliefs of a University of Mississippi sample. Three distinct eras of vaccine refusal were detailed, with the third (present) era distinguished by the use of the internet to spread anti-vaccine ideologies. The …

Method Development Toward The Analysis Of The Functionalization Of Proteins, Victoria Calcote

Honors Theses

Calmodulin is a ubiquitous small intracellular protein that functions as a receptor for regulatory calcium signals and thus regulates a multitude of physiological processes in organisms as diverse as yeast, fruit flies, and mammals. Since its structure and function have been extensively studied, our goal is to create a calmodulin biomaterial, with the ultimate goal of in situ drug delivery or as a scaffold for stem cell growth and differentiation. We plan to create this biomaterial by using Click Chemistry, the rapid creation of new heteroatom bonds through a copper-catalyzed azide-alkyne cycloaddition. The bonds are created by treating calmodulin as …

Variability In Antibacterial Chemical Defenses In Caribbean Sponges Of The Genus Aplysina, Savannah Stockton

Honors Theses

Coral reefs represent one of the most diverse ecosystems on earth, providing habitat for at least 25% of all marine species. Coral ecosystems are faced with many stressors, both physical and biological, that are causing their continuing decline. Understanding the impacts of these stressors, and the resilience of coral reefs, is important to the long-term survival of these critical ecosystems. As a major constituent of marine communities, sponges are an important phylum contributing to the ecology of coral reefs. Like other components of coral reef habitats, sponges also face a wide variety of biological stressors including pathogens, predators, and competitors. …

Intrinsic And Extrinsic Factors Mediating The Expression Of Alternative Mating Tactics In Male Green Treefrogs, Hyla Cinerea, Aleah R. Liddell

Honors Theses

Males of many anuran species optimize their chances of reproductive success by vigorously calling to attract females or by adopting a non-calling satellite mating tactic. Satellite males perch silently near calling males and attempt to intercept females that the caller attracts. Models predict that tactic expression in male anurans is influenced by either extrinsic factors (i.e., the social-acoustic environment) or intrinsic factors (i.e., circulating hormone levels and body condition), but it is unclear how these factors potentially interact to influence tactic expression. For example, males often adopt a satellite mating tactic in response to the broadcast vocalizations of conspecific males, …

Identification Of Inhibitors Of Fxii, Alexandra C. Bensel

Honors Theses

Hereditary angioedema is characterized by recurrent swelling of the extremities, larynx, and gastrointestinal tract. While type I and type II hereditary angioedema result from a C1-inhibitor deficiency, a new variant of the disease, type III hereditary angioedema, was recently discovered in individuals with functioning levels of the C1-inhibitor. Ongoing research has postulated that increased activity of factor XII is responsible for this new form of hereditary angioedema because it causes overproduction of bradykinin. The purpose of the experiment was to identify inhibitors of factor XII. One hundred and seventy six molecules were screened through a dose response using Human Factor …

The Effects Of Red Light On Blue-Light Phototropism In Arabidopsis Thaliana, Alison Michelle Neel

Honors Theses

Red and blue light pathways interact with one another to guide the phototropic growth and development of seedlings. Both roots and hypocotyls experience phototropism. This study aims to describe the effects of red light on blue-light phototropism and to examine the relationship between red and blue light pathways. Two wild-type ecotypes of Arabidopsis thaliana, germinated either under white light or in darkness, were exposed to different red and blue light conditions and used to examine these relationships. Red light exposure preceding growth under solely blue light was shown to have an inhibitory effect on root lengths, regardless of ecotype or …

Analysis Of Joint Dysfunction During An Aging- Simulation, Laboratory Exercise For Allied Health Students, Anna E. O'Connor

Honors Theses

The aim of this experiment was to (1) develop and assess a joint-dysfunction simulation learning and sensitization activity for a Human Anatomy and Physiology I lab on joint structure and function (Part A) and (2) quantify the range of motion experienced during the simulation (Part B). The lab protocol allowed 318 allied health students to experience some of the physical limitations of aging, specifically arthritis, by asking them to perform activities of daily living (ADLs) while wearing simulation equipment that inhibited their range of movement. In Part A, students completed a pre- simulation survey, which assessed the students' knowledge about …

Effect Of 5-Hydroxymethylcytosine Modifications On Stability Of Vegf I-Motifs, Bethany Summerford

Honors Theses

DNA i-motifs are a secondary structure of DNA formed at low pH by internal folding of strands rich in cytosine. This study focuses on trying to determine if the DNA from the vascular endothelial growth factor (VEGF) promoter, which can form an i-motif, can be stabilized with the epigenetic modification to 5- hydroxymethylcytosine in four separate locations on the DNA strand, and to what extent the degree of stabilization depends on the location of the modification. Stability of the i-motif was determined by measuring pKa and melting point (Tm) of each VEGF modification using circular dichroism spectroscopy. The modified samples …

Hormonal And Social Factors Influencing Alternative Mating Tactic Decisions In Male Green Treefrogs, Hyla Cinerea, Brooks Pratt

Honors Theses

In many anuran species (frogs and toads), males alternate between calling and a non-calling satellite mating tactic. Satellite males characteristically associate with calling males and attempt to intercept females that are attracted to the vocalizations of the calling host male. The prevailing hypothesis is that the decision to adopt a calling or satellite mating tactic is based on relative attractiveness — males are predicted to adopt a satellite mating tactic when the vocalizations of nearby males are more attractive than their own. In support of this hypothesis, previous studies have shown that satellite males can be induced to call by …