Digital Commons Network^™

Vascular Flora Of The Possum Walk Trail At The Infinity Science Center, Hancock County, Mississippi, Hanna M. Miller

Honors Theses

The North American Coastal Plain contains some of the highest plant diversity in the temperate world. However, most of the region has remained unstudied, resulting in a lack of knowledge about the unique plant communities present there. This flora catalogues the vascular plant species along the Possum Walk Trail on the southeast corner of the Infinity Science Center in Hancock County, Mississippi. Additionally, interpretive signs have been developed to display information about engaging species and phenomena to increase public interest in native plant life and involve them conservation efforts. The site was surveyed from spring of 2015 to spring of …

Compassion Fatigue And Educational Preparation Among Mississippi Child Welfare Workers, Meredith D. Mcphail

Honors Theses

The prevalence of trauma among youth involved with the child welfare system affects not only these individuals but also child welfare service providers, namely social workers, who experience this trauma indirectly through interaction with the children. This exposure places service providers at risk for compassion fatigue, in which these workers themselves experience trauma symptoms or are less able to complete job tasks. It is especially important to address the issues of negative personal reactions among this population in order to best care for social service workers in Mississippi and identify strategies to effectively improve Mississippi’s child welfare system. As such, …

Awareness Of Biological Mechanisms Of Artificial Sweeteners And The Effects Of Increased Awareness Via Video Media On Intent To Change Dietary Habits In Traditional College-Age Students, Cassie A. Mahler

Honors Theses

In the past, artificial sweeteners have been touted as weight loss solutions because they theoretically do not interact with the body’s metabolism. However, new research is being done to test this theory, but what is the public awareness of these compounds’ interactions with the body? The purpose of this study is threefold: to assess the current awareness of the biological mechanisms of artificial sweeteners in traditional college-age individuals; to assess the effectiveness of video education in increasing this awareness; and to assess how the increase of this awareness might lead to intentions to change dietary habits. The study consisted of …

A Comprehensive Study Of The Effects Of Chemotherapy On Friction Ridge Detail, M Mariel Lowe

Honors Theses

Past research and case studies have shown that chemotherapy drugs appear to lessen the quality of friction ridge skin, thereby impacting cancer patients who are already experiencing a number of unpleasant side effects. Palmar-plantar erythrodysaesthesia, more commonly referred to as Hand-Foot Syndrome (HFS), is a common side effect of many chemotherapy agents and includes redness, swelling, and peeling of the skin of the hands and feet. This syndrome has been linked to a number of cases involving fingerprint loss, including a prior longitudinal study that evidenced degradation in response to capecitabine, a common chemotherapy agent. This research builds upon the …

Pearls And Politics: White Clubwomen’S Activism In The Postwar South, Kelly E. Liles

Honors Theses

Elite white women’s organizations, such as the League of Women Voters, the Daughters of the American Revolution, and the American Association of University Women, provide a unique perspective on history. These political women’s clubs, which range from liberal to conservative, are discussed in the context of how they responded to the postwar era of McCarthyism and the Civil Rights Movement. These women wanted to become respected political actors; however, they understood this was only achieved in a manner that was considered acceptable for women. This study begins by analyzing who these women were, including their political inclinations and motivations and …

Facilitating Environmental Literacy In A Socio-Digital Landscape, Anna E. Hotard

Honors Theses

My work engages digital communications, traditional writing, and campus/community engagement in order to curate emergent knowledge of environmental citizenship and sustainable development. In the city of Hattiesburg, Mississippi, there has been a growing interest in environmental literacy both on the University of Southern Mississippi campus and within the city community. Environmental literacy takes many forms from informational sessions, fundraisers and events, to outright activism. This work informs my evolving identity as an editor as I choreograph traditional transcripts into a digital sphere in order to provide a collective space for related interests.

Developing A Deletion Construct Of The Halothiobacillus Neapolitanus Csos1c Gene, Ellyn K. Dunbar

Honors Theses

The purpose of this research was to develop a deletion construct for the chemoautotrophic bacterium Halothiobacillus neapolitanus, which will be used to generate a mutant lacking a carboxysome shell protein gene. The carboxysome is the location of carbon dioxide fixation. The operon that encodes the carboxysome contains three genes for CsoS1 proteins, the major components of the carboxysome shell. The small CsoS1 proteins self-assemble into hexamers with small central pores. The hexamers arrange into the facets of the icosahedral carboxysome shell. The pores are believed to be involved in selective diffusion of materials necessary for carbon dioxide fixation across the …

Synthesis And Application Of (R)-3-Methylpyrrolidine-3-Carboylic Acid, Shelby D. Dickerson

Honors Theses

L-Proline is an amino acid widely used in pharmaceutical and biotechnological research due to its catalytic activity and biological relevance. L-Proline has been recognized and utilized as an organocatalyst, which allows cleaner, more sustainable reactions. However, one issue with L-proline is its low solubility in organic systems, limiting its reactivity and efficiency, especially when considering industrial research. Two reactions that utilize L-Proline are the Michael and Aldol but require 100 mol% of L-Proline and 30 mol% of L-Proline, respectively. This research will focus on the synthesis of an analogue of L-Proline utilizing inexpensive, commercially available reagents. A variety of organic …

The Lipstick Effect Operates At The Level Of Automatic Visual Attention, Aaron M. Bermond

Honors Theses

Although general consumer spending usually decreases during economic recessions, Hill and colleagues (2012) found evidence that women concerned with economic recession demonstrated an increased preference towards purchasing products capable of enhancing physical beauty, thereby allowing them to appear more attractive to mates with resources. Referring to this phenomenon as the lipstick effect, these researchers suggested such purchasing preferences demonstrate evidence for an evolved female mating strategy. The current study was designed to more directly test whether the lipstick effect represents an evolved female mating adaptation by determining if it operates at the level of automaticity, specifically automatic visual attention (Fodor, …

Preparation Of Myod Mrna For The Differentiation Of Stem Cells Into Muscle Cells, Stephanie A. Ye

Honors Theses

Embryonic stem cells (ESCs) are characterized by their two special properties: pluripotency and self-renewal. Pluripotency is the ability of ESCs to differentiate into any cell type upon expression of specific proteins called transcription factors. In order to induce differentiation, transcription factors specific for the particular cell type have to be introduced and expressed in the ESCs. The introduction of transcription factors can be achieved by using either DNA, RNA, or protein. According to the Central Dogma of Molecular Biology, DNA is first transcribed into messenger RNA (mRNA), which is then translated to make a functional protein molecule. Among the available …

Exogenous Polyunsaturated Fatty Acid (Pufa) Scavenging Affects Phospholipid Profiles And Virulence Phenotypes In Vibrio Cholerae And Vibrio Parahaemolyticus, Anna Moravec

Honors Theses

AIM: This research had two primary objectives. First, this study sought to determine whether or not exposing Vibrio cholerae and Vibrio parahaemolyticus to polyunsaturated fatty acids (PUFAs) would lead to changes in their respective phospholipid profiles. Secondly, if membrane remodeling occurred, this study sought to determine the impact of membrane modifications on bacterial fitness by evaluating their effect on membrane permeability, biofilm formation, and response to stresses. METHODS: The Bligh and Dyer method was used to extract bacterial lipids, which were subsequently analyzed via thin-layer chromatography (TLC). The effect of fatty acids on bacterial fitness was assessed using stress, biofilm, …

More Than A Wheelchair In The Background: A Study Of Portrayals Of Disabilities In Children's Picture Books, Sarah Caroline Crawford

Honors Theses

A review of scholarly research conducted between 2004 and 2014 revealed that portrayals of disabilities in works of children’s literature were historically poor, containing stereotypical and negative characterizations. While noting some improvements, researchers still decry a lack of balance in roles of power between characters with disabilities and those without, a lack of depth in storylines and levels of character development, and disproportionate representations of disability categories, male and female characters, and cultural minorities in comparison to the true population (Altieri, 2006; Dowker, 2004; Dyches & Prater, 2005; Dyches, Prater, & Leininger, 2009; Golos & Moses, 2011; Golos, Moses, & …

Measuring Phytophthora Resistance Phenotypes In Segregating Testcross Families Of Hybrid American Chestnut Trees, Anna C. Robinson

Honors Theses

Phytophthora root rot (PRR), caused by the oomycete Phytophthora cinnamomi Rands, is a formidable obstacle to the restoration of Castanea dentata Marsh. commonly known as the American chestnut. Genetic resistance to PRR has been observed in Asian species of chestnut including C. mollissima Blume, and in interspecific hybrids between C. mollissima and C. dentata. We hypothesized that root rot resistance alleles would segregate in a 1:1 ratio within progeny of crosses between PRR resistant F1 hybrids and PRR-susceptible American chestnut trees (first-backcrosses), and that PRR resistance could be successfully passed down to all families of first-backcross hybrids. To test these …

The Transfer Of Commensal And Transient Bacteria Into Deep Tissue Using Dry Needling Needles Through Agar Media Designed To Mimic The Elasticity Of Human Skin, Denisse N. Breton Guerrero

Honors Theses

Needle insertion into skin for the purpose of alleviating muscle pain began with the emergence of traditional Chinese medicinal (TCM) acupuncture. “Dry needling”, a recent alternative to acupuncture, involves inserting a monofilament needle down into the deep tissue of a patient’s skin in order to reach the affected muscle. Despite its effectiveness and low risk of infection, dry needling remains questionable with respect to its safety and the lack of research to confirm the low risk of bacterial transmission via the dry needling needles. In this microbiological study, highly concentrated Tryptic Soy Agar was utilized to simulate the elasticity of …

Alkaloids In E-Cigarettes: Their Effects On Cell Growth And Gene Regulation, Maxwell H. Marlowe

Honors Theses

E-cigarettes have become increasingly popular in the past decade. They are marketed as smoking cessation aids. These products have not been well regulated or researched with respect to health concerns and safety issues. A number of toxic compounds have been discovered in refill solutions, and the long-term effects of e-cigarette use are still largely unknown. Nicotine is one of the primary alkaloids within e-cigarette refill solutions. Nevertheless, other tobacco alkaloids are present including; cotinine, myosmine and anabasine, even though these compounds are not disclosed on the packaging. This study uses known amounts of tobacco alkaloids, in an in vitro culture …

A Chemical Sensor For Cyanide, Rachel E. Lambert

Honors Theses

The cyanide ion and its gaseous form, hydrogen cyanide, are extremely toxic. Cyanide impairs cellular respiration by inhibiting cytochrome c oxidase, an enzyme in the electron transport chain, leading to cell death.

In a previous study, we synthesized an optical sensor that detects cyanide selectively. The aim of this project is to increase the sensitivity of this sensor. This can be achieved by utilizing the unique spectroscopic properties of lanthanide ions.

The lanthanide metal (europium or terbium) was added to a coumarin-glycine chemodosimeter in a DMSO solvent system. The sensor was titrated with several monodentate analytes including, nitrate, octylamine, 1-pentanethiol, …

Painless Hematophagy: The Functional Role Of Novel Tick Metalloproteases In Pain Suppression, Joseph W. Jelinski

Honors Theses

Ticks secrete a plethora of pharmacologically active molecules in their saliva while feeding. These allow the tick to feed upon a host over prolonged periods of time in an itch free and painless attachment. The exact mechanism of pain suppression by the tick has barely been investigated. In this study, two angiotensin converting enzymes (ACEs), members of the metalloprotease family, are identified as potentially responsible for the degradation of pro-inflammatory peptides. It is hypothesized that these tick ACEs block the function of bradykinin through degradation of the peptide, contributing to the tick’s ability to conduct a pain and itch-free host …

Using Intrinsic Properties Of Polyaniline To Sense Expression Of The Microrna Let-7, Jared N. Gloria

Honors Theses

MicroRNAs are approximately 22-nucleotide long RNA molecules that function through decay and translational repression of messenger RNA. The microRNA let-7 is found to play a role in maintaining the fate of differentiated cells in humans. Thus, expression level of this microRNA is a reliable biomarker of tumor cell phenotype. However, there are significant limitations in the current profiling techniques of microRNA. The current methods like northern blotting, microarrays, RT-PCR, or using locked nucleic acid (LNA) for in-situ hybridization are either laborious, semi-quantitative, or expensive. In this research we try to address this issue by developing a fast, specific, and inexpensive …

The Use Of Hydrotherapy In Labor To Promote Physiologic Labor, Nicolette Johnson

Honors Theses

The pain of labor is well understood, yet, very difficult to treat. This is secondary to the fact that each woman’s perception of pain varies as it is influenced by many different factors. According to Lowedermilk, Perry, Cashion, & Alden, (2012) how the woman perceives pain is “influenced by a variety of physiologic, psychological, emotion, social, cultural and environmental factors” (p. 387). A nurses’ role is to act as a patient advocate and with that entails maintaining the patient’s comfort throughout the labor process. In order to do so, a nurse must be able to assess the patient from not …

How Recipient Age Affects Long Term Survivability In Heart Transplantation Patients, Daniel Baker

Honors Theses

Heart transplantation is very traumatic for the human body. It involves physically taking out an organ that is vital for normal function and replacing it with an organ that is foreign to the body. However, it is sometimes thought that this is a very well known and well-researched procedure, when the reality is that the very first heart transplant took place less than 50 years ago by Dr. Christiaan Banard (Brink & Hassoulas, 2009). During the last 50 years many breakthroughs have been discovered and the procedure has been vastly helped by the rise of immunosuppressant’s and new surgical technology, …

Mindfulness And Prevention In Western Medicine: Focus On Physician Assistant, Amber Bernelis

Honors Theses

The health and wellness of the mind has been an important aspect that has been recognized by different cultures and medical traditions for thousands of years, but was generally neglected in the United States until recently. The beginning of the western mindfulness revolution started when Jon Kabat-Zinn introduced the concept and practice of mindfulness to care for the health and wellness of our mind. “Mindfulness is a simple concept in which one pays attention on purpose, in the present moment, nonjudgmentally” (Kabat-Zinn, 1994, p. 4). Research indicates that mindfulness helps to treat aspects of chronic diseases, pain, as well as …

Bioaccumulation Of Iron In Gambusia Affinis In Lemna Minor Mediated Surroundings, Canaan Sutton

Honors Theses

No abstract provided.

Behavioral Sensitization Following Concurrent Exposure To 4-Methylmethcathinone (4-Mmc) And 3,4-Methylenedioxymethamphetamine (Mdma) In Male Sprague-Dawley Rats, Trent Bullock

Honors Theses

Recreational use of a new class of stimulant drugs known as synthetic cathinones is a recent public health concern. Although the Drug Enforcement Administration placed several of the most common of these substances permanently on schedule 1, their use is still prevalent as they remain low cost, accessible, and potent. Concomitant use of cathinone derivatives with other psychostimulant drugs is commonly reported by recreational users. Despite the prevalence of synthetic cathinone abuse, there is currently a paucity of scientific research regarding the behavioral and neurochemical effects of these drugs in mixtures with other drugs of abuse. The behavioral sensitization paradigm …

Effects Of Creatine, Leucine, And Ethanol On Glial Cell Line-Derived Neurotrophic Factor In C2c12 Skeletal Muscle Cells, Daniel Waters

Honors Theses

Glial Cell Line-Derived Neurotrophic Factor (GDNF) is a protein expressed throughout the mammalian nervous system that serves several protective functions. Originally discovered in the brain, where it supports the survival of dopaminergic neurons, GDNF has since been shown to be expressed in skeletal muscle and is vital for the maintenance of motor neurons. The relationship between muscle hypertrophy, and GDNF is not well understood. The purpose of this study was to examine the relationship between hypertrophy and GDNF by inducing hypertrophy in C2C12 skeletal muscle cells using leucine and creatine, inhibiting hypertrophy using ethanol and measuring GDNF content. As measured …

Bacteriophages That Can Lyse Antibiotic Resistant Bacteria, Michael Monaco

Honors Theses

A Klebsiella pneumoniae bacteriophage was isolated from waste water and purified over multiple rounds of plaque removals and serial titrations. This bacteriophage was imaged in a transmission electron microscope and then tested with selected antibiotics for effectiveness in reducing optical density of K. pneumoniae colonies. The phage was shown to have an increased effectiveness in reducing the optical density when used together with an antibiotic that the bacteria was susceptible to.

The Clean Power Plan: A Legal Analysis Of The E.P.A.'S Final Rule, Robert Paul

Honors Theses

The consensus among scientists is that climate change and its consequences are upon us as the result of human activity. The necessary reductions of emissions of greenhouse gases to mitigate these consequences have long been identified. The continued failure of the United States Congress to pass any national legislation directly addressing climate change has led President Obama to pursue executive action, through the authority of the Environmental Protection Agency, to regulate greenhouse gas emissions under the Clean Air Act section 111(d). Collectively known as the Clean Power Plan, the heart of this action is the carbon dioxide emissions Final Rule …

Global Paths To Enlightenment: A Foundation Scholar Study Abroad To The Dominican Republic, Tacy Allan

Honors Theses

There are many different ways for students to gain a multicultural awareness and a global focus, which are important tools for their lives. Study abroad opportunities are seen as one of the widest-reaching and best hands-on learning paths that can be taken to broaden the horizons of students. This proposal combines service learning, a social justice issue (clean water), and global impact; giving students the chance to learn what is here in Kalamazoo and what is in Dominican Republic, how those issues compare and contrast as well as how each location is dealing with these issues. This proposed class will …

"Patterns In The Abundance Of Benthic Algae In Streams", Courtney Wright

Honors Theses

Benthic, microscopic algae are the main drivers of primary productivity in streams. The relationship between the abundance and productivity of benthic algae in streams and lakes and the availability of limiting nutrients, especially soluble reactive phosphorus (SRP), has been extensively studied (reviewed in Allan and Castillo 2007, Larned 2010). However, nearly all of this research has been conducted on the periphyton assemblage found on hard substrates (e.g., stones, wood), while very little work has addressed the factors affecting the abundance of epipelic algae (i.e., the algae associated with depositional (soft) sediments such as sands and silts). One of the few …

The Effects Of Aging And Estrogen Treatment During Aging On The Rat Epididymis, Kathryn Davis

Honors Theses

Previous studies from our lab have demonstrated that a normal estrogen environment in the testis contributes to maintaining spermatogenesis in adult rats and that estrogen treatment can attenuate the age-associated decline in sperm production. The purpose of this study was to determine if the estrogen environment of the epididymis is altered with age and what effects estrogen treatment may have on the epididymis during aging. The study compared untreated rats at 15 months of age to 18 month old rats that were treated with either vehicle, or estradiol- valerate, once every third day from 15 to 18 months. Mean concentrations …

A Guided Inquiry To The Bromination Of Alkenes: An Effort To Foster Student Understanding Of Thin Layer Chromatography In The Organic Chemistry Laboratory, Casey Wright

Honors Theses

Many of the chemistry laboratories taught at Western Michigan University are “cookbook” labs, meaning that students are given an instruction manual with a prescribed set of instructions and a predicted outcome. Therein we present an introductory organic chemistry laboratory experiment to reinforce the student understanding of Thin Layer Chromatography which utilizes a guided inquiry format as part of an effort to move toward discovery based learning in our organic chemistry curriculum. Educational research was performed on classroom artifacts collected from the laboratory. We were able to gain an understanding of the struggles students face in applying general chemistry content knowledge …