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The Structural And Functional Properties Of A Double Mutant Of Human Acidic Fibroblast Growth Factor (Hfgf-1), Arwa Mohammed Alghanmi

Graduate Theses and Dissertations

Human acidic Fibroblast Growth Factor 1 (FGF-1), a member of the FGF superfamily, is a potent mitogen and heparin-binding protein involved in a broad spectrum of biological processes, including angiogenesis, cell proliferation, and wound healing. Design of hFGF-1 with an increased thermal stability and an enhanced cell proliferation activity is highly desired for wound healing applications. Herein, we have designed the variant of FGF-1 by substituting two important amino residues in the heparin-binding pocket. The variant was overexpressed in Escherichia coli and was successfully purified to homogeneity using an affinity chromatographic procedure. Far-UV circular dichroism spectroscopic analysis showed that the …

A Laboratory-Scale Study On The Production Of High-Value Products From Broiler Litter Involving Solid-State Anaerobic Digestion And Mushroom Cultivation, Joe Barrett Carter Iii

Graduate Theses and Dissertations

There is a need to investigate alternate uses and treatments of broiler litter that lessen environmental impacts and decrease costs associated with its disposal. Anaerobic digestion is a biological process in which organic material is converted to a renewable fuel source. However, the substrate for anaerobic digestion often requires some form or pretreatment. Certain types of fungus have been investigated as a pretreatment for anaerobic digestion, one of which is Pleurotis ostreatus or the oyster mushroom, which also produces an edible fruiting body. Thus, this study was performed to investigate the use of broiler litter for oyster mushroom cultivation and …

The Effect Of Blinded Hydration State On Thermoregulation And Performance In Male Cyclists, Jon David Adams

Graduate Theses and Dissertations

Purpose: The purpose of these studies was to observe the effect of dehydration on exercise performance while subjects were blinded to their hydration status. Methods: Study 1: Seven male cyclists (weight: 71±8 kg, body fat: 14±6%, VO2peak: 59.4±6 ml∙kg-1·min-1) exercised for 2 hours on a cycle ergometer at 55% VO2peak, in a hot-dry environment (35°C, 30% rh), with a nasogastric (NG) tube under euhydrated (EUH-NT) and hypohydrated (DEH-NT) conditions. In both trials, thirst was matched by drinking 25 mL every 5 min. In the EUH-NT trial sweat losses were fully replaced via the NG tube. Following the 2 hours of …

Civic Tenderness: Love's Role In Achieving Justice, Justin Leonard Clardy

Graduate Theses and Dissertations

Martha Nussbaum’s work Political Emotions: Why Love Matters for Justice identifies the role that compassion plays in motivating citizens in a just society. I expand on this discussion by considering how attitudes of indifference pose a challenge to the extension of compassion in our society. If we are indifferent to others who are in situations of need, we are not equipped to experience compassion for them. Building on Nussbaum’s account, I develop an analytic framework for the public emotion of Civic Tenderness to combat indifference.

Civic tenderness is an orientation of concern that is generated for people and groups that …

Structural Characterization Of Bacterial Collagenases, Ryan Bauer

Graduate Theses and Dissertations

Clostridium histolyticum secretes collagenases ColG and ColH to cause extensive tissue destruction during myonecrosis. The collagenases are multi-domain enzymes consisting of a N-terminal collagenase module, s1, polycystic kidney disease (PKD)-like domains (s2 in ColG; s2a and s2b in ColH) and collagen-binding domains (CBD) (s3a and s3b in ColG; s3 in ColH). The individual CBD and PKD-like domains chelate calcium to modulate stability and domain rearrangement. Though used by bacteria to break down collagen in the extracellular matrix during infection, therapeutic use of the enzyme to break down excess connective tissue has been approved by the FDA. Meanwhile, the use of …

Effects Of Chemical And Enzymatic Modifications On Starch And Naringenin Complexation, Ana Isabel Gonzalez Conde

Graduate Theses and Dissertations

Naringenin is a flavanone naturally present in grapefruit and tomato skin, which has been demonstrated to have health benefits. However, because of the low water solubility and bioavailability, naringenin applications are limited. Starch inclusion complexes have been shown to improve the solubility and bioavailability of poorly water soluble bioactive compounds. The present study aimed to prepare and characterize complexes of naringenin with starches, including potato starch and high amylose corn starch (Hylon VII), which were chemically (acetylation or hydroxypropylation) and enzymatically modified (debranched or debrahced/β-amylase treated). Soluble and insoluble complexes were recovered, and their physicochemical properties were characterized. The treatments …

Characterization Of Nanoparticles Using Solid State Nanopores, Santoshi Nandivada

Graduate Theses and Dissertations

Solid state nanopores are widely used in detection of highly charged biomolecules like DNA and proteins. In this study, we use a solid state nanopore based device to characterize spherical nanoparticles to estimate their size and electrical charge using the principle of resistive pulse technique. The principle of resistive pulse technique is the method of counting and sizing particles suspended in a fluid medium, which are electrophoretically driven through a channel and produce current blockage signals due to giving rise to a change in its initial current. This change in current is denoted as a current blockage or as a …

Effects Of Filtration Conditions On Clearance Of Bacteriophage, Rigen Te

Graduate Theses and Dissertations

Virus Clearance is one of the major challenges in biopharmaceutical industry, especially in the manufacturing of drugs. U.S. Food and Drug Administration (FDA) recommends using several model bacteriophages as surrogates of pathogenic mammalian virus for process validation. In this work, two bacteriophages were tested against 30 kDa, 100 kDa, 300 kDa polyethersulfone (PES) membranes to investigate the effects of pore size on virus clearance. Virus particles were spiked into the protein feed solutions containing bovine serum albumin (BSA) or lysozyme at different concentrations. Besides protein concentration, the effects of feed pH on the filtration performance and virus rejection were also …

Creating Sustainable Supply Chains: Influencing Sustainable Practices In The Supply Chain, Saif Mir

Graduate Theses and Dissertations

Organizations worldwide are reporting their commitment to create sustainable supply chains. Ultimately, supply chain professionals are the drivers of change within their respective organizations, so this dissertation examines the role of communication as a tool to persuade supply chain professionals to create sustainable supply chains. The three studies within this dissertation employ different methodologies to examine the role of communication in the development and implementation of sustainability initiatives. The first study, a grounded theory investigation, highlights the network, communication, and structural factors that provide a strong business case for the development of sustainability initiatives. A strong business case influences the …

Off The Grid, Matthew Owen Buffington

Graduate Theses and Dissertations

Off the Grid explores the messy relationship between public and private perceptions of our urban spaces, especially the tensions created when lived experience runs up against the physical and conceptual networks of cities: street grids, construction tape, and property lines. Incorporating different modes of spatial representation, from cartographic diagrams to isometric illustrations and Renaissance perspectives, this exhibition examines the role drawing plays in how we conceptualize the divisions and definitions of everyday space. The drawings engage the often overlooked detritus of city life, from layers of old graffiti to overgrown dirt piles and unmoored electrical wiring, that complicate our understanding …

Rock Art Management And Landscape Change: Mixed Field Assessment Techniques For Cultural Stone Decay, Kaelin M. Groom

Graduate Theses and Dissertations

As tourism continues to grow as one of the world’s most ubiquitous markets, the development and promotion of non-invasive techniques for cultural stone decay analysis and landscape change are vital to establishing conditional base-lines to best aid cultural heritage management (CRM) efficacy. Using rock art as a medium, this dissertation presents three independent case studies employing the Rock Art Stability Index (RASI) and repeat photography to explore the merits of mixed rapid field assessment techniques in relation to CRM and heritage tourism. While rock art is only one example of irreplaceable world heritage resources, examining how they decay and what …

Dinner, Daniel Reuben Baskin

Graduate Theses and Dissertations

Dinner is an interactive exhibition which presents appropriated works of art collected and hung in a clustered salon style, as well as a fully realized recreation based on a 16th century Dutch banquet still-life, which presents guests with meats, cheeses, fruits, vegetables, breads, and wine to share and imbibe. Dining ware is provided for guests at the entrance to the exhibit, as are suggested topics of conversation, which are presented on slips of paper for guests to carry with them throughout their time in the space. Within the collection of wall-mounted works are references to ancient Greek and Roman marble …

"A Magic Deeper Still": Sacramental Poetics In William Wordsworth, Christina Rossetti, And C.S. Lewis, Eric Michael Bontempo

Graduate Theses and Dissertations

A sacramental poetics requires a particular mode of being-in-the-world. Religiously-minded poets, from Dante and Milton to Donne and Herbert, have long considered how the individual becomes attuned with creation and God’s will. But what happens when modernity and secularization challenge long-held assumptions about the universe and how humankind fits into it? A reevaluation is then needed. My thesis begins with an examination of how William Wordsworth, who sort of falls into modernity, seeks to reoccupy the functions of religion in an increasingly secularized landscape. One consequence of the European Enlightenment is the disentangling and distancing that occurs in regards to …

Central Role Of Vasotocin In The Neuroendocrine Regulation Of Stress Responses And Food Intake In Chickens, Gallus Gallus, Gurueswar Nagarajan

Graduate Theses and Dissertations

It is well known that arginine vasotocin (AVT) in birds is involved in physiological homeostasis such as cardiovascular, osmotic regulation as well as reproductive functions. Pertinent to these physiological functions, AVT immunoreactive (-ir) neurons in the hypothalamus have been found associated with hemorrhage, dehydration, oviposition and other physiological regulation. Evidence, however, suggests that AVT also plays significant roles in modulating behavior, memory, stress, and food intake. This dissertation research addresses the latter two neuroendocrine functions of AVT in detail within the chicken brain. First, the functional role of AVT-ir neurons in conjunction with corticotropin releasing hormone (CRH)-ir neurons in the …

Identification Of Molecular Signatures Of Environmental And Nutritional Stress Responses In Poultry, Phuong Hieu Nguyen

Graduate Theses and Dissertations

Poultry production plays an important role in the food supply and security for billions of people. Poultry meat is considered the most efficient, nutritious source of protein, without religious taboos, and is relatively inexpensive. However, the poultry industry is facing substantial challenges. The global environmental temperature has experienced dramatic changes in the last few years. Modern broilers are not well adapted to environmental challenges (heat and cold stress), which have resulted in heavy economic loss in the poultry industry worldwide. In addition, the use of feed restriction regimens in management of breeders as well as the transportation of young chicks …

Influence Of Stream Permanence, Predation, And Invasive Species On Crayfish In The Ozark Highlands With An Emphasis On Species Of Greatest Conservation Need (Orconectes Marchandi, Orconectes Eupunctus And Cambarus Hubbsi), Allyson Noelle Yarra

Graduate Theses and Dissertations

Due to the ecological importance of crayfish and the increasing vulnerability of freshwater systems due to habitat loss, invasive species, and hydrologic alteration, understanding crayfish-environment relationships is crucial in the context of aquatic species conservation. I sought to examine the influence of hydrologic variation among intermittent and permanent streams on crayfish occupancy, abundance, predation risk, and potential vulnerability to invasive species effects. I conducted crayfish and environmental data sampling during two consecutive summers across 20 Ozark streams of differing permanence levels (10 intermittent, 10 permanent). In these same streams, I conducted fish and scat surveys over the course of four …

Flight Activity, Oviposition Pit Distribution, And Emergence Densities Of Monochamus Titillator And M. Carolinensis In The Ozark-St. Francis National Forest In Arkansas, Jake Bodart

Graduate Theses and Dissertations

Monochamus (Coleoptera: Cerambycidae) are a genus of longhorn beetles commonly known as pine sawyers. They have a worldwide distribution coincident with pines (Pinaceae) and are vectors of the pinewood nematode (Bursaphelenchus xylophilus). In the United States, there are eight known Monochamus species and they have a sympatric distribution with at least one other Monochamus species throughout their range. Monochamus are known to attack stressed, dead, and dying conifers especially pines. In the Ozark- St. Francis National Forest in Arkansas, there are two species of pine sawyers and they share this sympatric distribution observed throughout the United States, M. titillator (southern …

Cultural Reimagining And Literary Voice: Southeastern Tribal Women Negotiate Cultural, Social, And Political Identity Through Literature, Linda Sue Shaffer

Graduate Theses and Dissertations

This dissertation examines literature written by women who identify with Native tribes that originally inhabited, and in some cases continue to inhabit, the southeastern area of what is now known as the United States. The analysis presented in each chapter applies tribally specific methods used for creating knowledge within the particular discourse community being represented through literature. The project also employs the perspectives of Native literary scholars to consider the ways in which the roles and lives of Native women have been influenced by Euro-American values and to analyze the ways in which these female authors engage literature as a …

Phenology, Habitat Use, And The Impacts Of Wetland Management On Autumn Migrating Rails In Missouri, Auriel M.V. Fournier

Graduate Theses and Dissertations

Rails (Family: Rallidae) are among the least studied birds in North America, in large part due to their elusive nature. As a wetland-dependent species, understanding the timing of their migration and their habitat needs during migration is especially important since management needs to be timed to balance the needs of many species. I developed and verified a new distance sampling based nocturnal ATV spotlight survey because traditional call-broadcast surveys are not effective during autumn migration because of the drop off in call rate after the breeding season. These surveys allow us to ask point-level questions about what habitats rails select …

The Use Of Probiotics To Control The Microbial Load Present In Commercial Broiler Chicken Hatch Cabinets As An Alternative To Formaldehyde Fumigation, Lucas Elzie Graham

Graduate Theses and Dissertations

Formaldehyde has been used for decades as a disinfectant in the poultry hatchery. Hatch cabinets are treated with formaldehyde to control the microbial bloom that occurs inside of the hatch cabinet as the hatch progresses. Even with formaldehyde being a known human carcinogen and the detriment that it has on living creatures millions of chicks are exposed to formaldehyde in the hatch cabinet. In these experiments we tested a lyophilized probiotic spray inside of the hatch cabinets from day nineteen to day twenty one to control the microbial bloom that occurs. Hatch cabinet environments were sampled in six experiments. Media …

In Ovo Evaluation Of Floramax-B11 On Marek's Disease Hvt Vaccine Protective Efficacy, Hatchability, Microbiota Composition, Morphometric Analysis, And Salmonella Enteritidis Infection In Broiler Chickens, Kyle Dean Teague

Graduate Theses and Dissertations

It has previously been shown that effective probiotics can accelerate gut maturation and the development of a normal microflora in poultry. This results in increased resistance to enteric pathogens encountered by chicks early in life. Our objective in experiments 1 and 2 was to evaluate the effect of in ovo administration of FloraMax®-B11 (FM) on Marek’s disease (MD) herpesvirus of turkeys (HVT) vaccine protective efficacy. In Exp. 1, day 18 White Leghorn embryos were randomly distributed in four groups, 1) HVT vaccinated, no MDV challenge, 2) HVT + FM vaccinated, no MDV challenge, 3) HVT vaccinated, challenge with virulent MDV, …

Wayward Women, Macho Men: Linguistic Construction Of Gender Binaries In Yxta Maya Murray's Locas And Denise Chavez's Loving Pedro Infante, Stephanie Tangman

Graduate Theses and Dissertations

Language labels and defines in order to enhance meaning and communication, but through these labels and definitions speakers are also conditioned to associate certain connotations with words and, therefore, their referents. While often harmless, linguistic conditioning can at times create unsavory associations with these referents. One of these instances occurs in gendered labels and conceptions of male and female bodies and purpose. Both Yxta Maya Murray’s Locas and Denise Chavez’s Loving Pedro Infante can be read through a lens that applies linguistic conditioning with gender theory in order to examine the reinterpretation of female archetypes in the Chicana imagination. It …

Development Of A One Pass Microwave Heating Technology For Rice Drying And Decontamination, Deandrae Lynette Smith

Graduate Theses and Dissertations

An industrial microwave (MW) system operating at 915 MHz frequency was used to dry high moisture content (MC) (23% to 24% wet basis) medium-grain rough rice samples (cv. Jupiter). The rice beds were contained in a modified tray that accommodated up to 9 kg of rice separated by thin fiberglass mesh in 3 kg increments. Each layer of rice was fitted with fiber optic sensors connected to a real time data logger during MW treatments. It was determined that drying rice to a MC of 14% to 16% was feasible with the application of MW specific energy at 600 kJ/kg-grain …

Evaluation Of The Correlation Between The Oxidation Reduction Potential And Free Chlorine Residual For Different Chlorine Sources Used In Poultry Drinking Water Sanitation, Samantha Renee Cox

Graduate Theses and Dissertations

A critical component in commercial poultry production is to ensure birds are provided clean, quality water. Multiple disinfectants can be utilized to optimize a good water quality program. The goal of these water disinfectants is to greatly reduce or eliminate the presence of all bacteria. In recent years, the oxidation-reduction potential (ORP) meter has been a tool utilized by the poultry industry to monitor chlorine efficacy in drinking water. An ORP reading of 650-750 millivolts (mV) has become the industry standard for assuring an acceptable sanitizing residual of free chlorine is present for controlling microbial contamination regardless of the actual …

Exploring Winery Operation As A Diversification Option For Native American Tribal Enterprises, Randi M. Combs

Graduate Theses and Dissertations

The purpose of this research is to examine the perceptions of tribal members regarding the strengths, challenges, and opportunities presented by tribal winery operation. Specifically, issues of business diversification, marketing, perceived barriers to success, potential benefits to the tribe, and the role of agriculture in the preservation of tribal heritage were considered. A modified mixed-methods exploratory sequential research model was used to collect and organize data in two phases. Phase 1 quantitative data was used to inform the development of a Phase 2 qualitative interview protocol. Phase 1 found a significant relationship between a higher income level and a lower …

Indigenous Resistance: Settler-Colonialism, Nation Building, And Colonial Patriarchy, Megan E. Vallowe

Graduate Theses and Dissertations

“Indigenous Resistance: Settler-Colonialism, Nation Building, and Colonial Patriarchy,” interrogates the Western Hemisphere’s spatial construction by settler-states, Indigenous nations, and activists groups. In this project, I assert that Indigenous/Settler contact zones are significantly more convoluted than current scholarship’s use of contact zones in that the distinctions between Indigenous actors and settler-colonial ones are often blurred. These hybrid contact zones sometimes contain negative outcomes for all participants and often include undercurrents of insidious power dynamics within and across settler-states and Indigenous peoples alike. Using critical cartographic theory and deconstruction methods, this project first illustrates how empires ascribed a racialized patriarchy onto the …

The Effect Of Insects On Seed Set Of Ozark Chinquapin, Castanea Ozarkensis, Colton Zirkle

Graduate Theses and Dissertations

Ozark chinquapin (Castanea ozarkensis), once found throughout the Interior Highlands of the United States, has been decimated across much of its range due to accidental introduction of chestnut blight, Cryphonectria parasitica. Efforts have been made to conserve and restore C. ozarkensis, but success requires thorough knowledge of the reproductive biology of the species. Other Castanea species are reported to have characteristics of both wind and insect pollination, but pollination strategies of Ozark chinquapin are unknown. Experiments were conducted to determine the influence of insects on successful pollination of C. ozarkensis, as measured by production of burs and seeds. Exclosure treatments …

Influence Of Exogenous Phytase Level And Interaction With Supplmental Xylanase On Live Performance And Phosphorus Retention Of Broilers, Peyton Gilbert

Graduate Theses and Dissertations

Two 3-week experiments were conducted, each using 480 day-old male broilers placed randomly among 96 battery cages (5 per cage; 680cm2 per bird), to evaluate the influence of phytase supplementation, with or without supplemental xylanase, on bird performance. Individual body weights and cage feed weights were taken on days 0, 7, 14, and 21, and mean BW, body weight gain (BWG), feed intake (FI), and FCR over each period calculated. Mortality was recorded daily and used to calculate livability and adjust FCR in both trials. In trial 2, excreta was collected by cage from 14 to 21 days for bone …

The Effect Of Omega-3 Fatty Acids On Energy Metabolism, Energy Intake, And Metabolic Response In Normal Weight And Overweight And Obese School Aged Children (8-12 Years), Charlayne Mitchell

Graduate Theses and Dissertations

Background: Obesity is a major health concern in the United States. Omega-3 fatty acids (O3FA) have been observed to improve metabolic health and therefore might be useful in treatment of obesity. However, little is known regarding the effect of O3FA on school aged normal weight and overweight children.

Objective: The objective of this thesis was to determine if habitual intake of O3FA at breakfast improves energy metabolism, appetite, and metabolic response in overweight and obese school-aged children.

Design: Twenty healthy, normal weight (NW; n = 11) and overweight (OW; n = 9) children aged 8-12 years were randomly assigned to …

Where In The World Are Pallas's Fish Eagles? Migration And Ecology Of Haliaeetus Leucoryphus In Asia, Marla Lynn Steele

Graduate Theses and Dissertations

Pallas’s Fish Eagle (Haliaeetus leucoryphus) is the Northern Hemisphere’s least understood eagle species. Virtually nothing is known concerning the species’ ecology. Historically, Pallas’s Fish Eagles were expected to breed in three separate populations in Mongolia, China, and India and was considered one of the most common raptors in Asia prior to the 1900’s. However, by 1960’s major declines were observed. The species is currently listed as “globally vulnerable” by the International Union for the Conservation of Nature (IUCN) Red List. The current study examined over a century of Pallas’s Fish Eagle observation data to determine occupancy and detection rates throughout …