Digital Commons Network^™

Conformational Investigation Of Biopolymers Through Molecular Dynamics (Md) Simulations And In-Droplet Hdx-Ms Utilizing Cvssi, Samira Hajian Foroushani

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the last two decades, mass spectrometry (MS) has received significant interest in biological study platforms, especially in the areas of proteomics and metabolomics. With the development of soft ionization methods like electrospray ionization (ESI), mass spectrometry has emerged as a potent method for directly characterizing proteins in their native state. The recent invention of capillary vibrating sharp-edge spray ionization has opened new avenues for the study of biopolymer native structure including the usage of droplet charge tuning and in-droplet hydrogen-deuterium exchange (HDX) labeling. With this new technique comes the opportunity to study biopolymer ion structure preservation with molecular dynamics …

Machine Learning-Assisted Multiscale Simulation And Design Optimization Of Composite Jackets Under Low-Velocity Impacts, Masoud Mohammadi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research presents the development of a data-driven machine learning-assisted approach to simulate and optimize the design of composite materials subjected to low-velocity impacts. It focuses on a specific type of hybrid composite comprised of fiberglass and Kevlar fabrics stitched with Kevlar threads. The study begins by creating a multiscale finite element simulation for the composite under a low-velocity impact, which operates across three scales: microscale, mesoscale, and macroscale. This simulation accepts various inputs, such as different layer configurations and orientations, and provides impact outputs including maximum load and energy absorption capacity and displacement at failure.

Python and MATLAB scripts …

Overturning Race-Conscious Admissions: Analyzing The History Of Higher Education Affirmative Acton Legal Discourse And Preserving Whiteness, Hiba A. Qari

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although researchers have investigated Affirmative Action, few have attempted to understand and explain the language mechanisms shaping public opinion and policy. The purpose of this study was to explore the historical context of higher education Affirmative Action legal discourse. This study employed Critical Discourse Analysis and Critical Race Theory to analyze how language and race, as social constructs, maintain White dominance in law and higher education. The thematic concepts included power, White normativity, and diversity rhetoric. This project explored the historical evolution of the U.S. Supreme Court’s decisions on Affirmative Action policy in higher education. The study also investigated the …

An Inhibitory Recurrent Network In The Olfactory System, Farzaan Salman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neuromodulatory circuits are a necessary component of neural flexibility as they are able to act differentially depending on the receptors expressed by their downstream targets. Identifying the synaptic partners of modulatory neurons and determining the neurotransmitter and receptor content of these partners is necessary to identify the rules governing neuromodulatory circuits. In this study, I characterized the circuit of the contralaterally projecting, serotonin-immunoreactive deutocerebral neurons (CSDns), the sole synaptic source of the neuromodulator serotonin in the olfactory circuit of Drosophila melanogaster, by identifying the strongest synaptic partners of the CSDns, and the expression of genes encoding neurotransmitters and receptors of …

Weighted Effect Of Concentrated Cracking On The Structural Integrity Of Concrete Bridge Decks, Md Ala Uddin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Concrete bridge decks are vital components of transportation infrastructure, yet their deterioration due to aging, environmental, and operational factors poses significant challenges to maintenance and safety. In the United States, approximately 9.1% of bridges require improvement, with millions of daily trips relying on structurally deficient bridges. Cracking is one of the most frequently observed defects on concrete bridge structures. Traditional bridge inspections evaluate cracks based on severity (crack width) and extent (crack area), combining these values with other performance indicators to assess overall bridge health. However, the effect of concentrated cracking in localized areas on bridge integrity remains largely unexplored. …

Effect Of Specific Data Variations On Automated Speaker Recognition, Ethan David Meighen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Speaker recognition is not a new biometric modality but there are still many obstacles in the way in order for it to become as used as fingerprint recognition, facial recognition, and iris recognition. Many real-world environmental conditions, hardware device variations, and human behavior present serious challenges to the use of opportunistic voice or speaker samples for identification purposes. Non-idealities, identified as nuisance factors, include environmental noise, input device quality, length of utterance, sample rate variation, and unscripted data are common nuisance factors that can impact speaker recognition match score performance. The impact of the nuisance factors listed above were evaluated …

Investigation Of L-Serine Synthesis And Transport As Stabilizing Feedback Mechanisms Within The Tsetse-Wigglesworthia Symbiosis, Ryan Phillips

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Glossina morsitans), which are the obligate vectors of Old-World trypanosomes (Trypanosoma spp.) that cause African trypanosomiasis, have a bacteriome that houses the intracellular endosymbiont Wigglesworthia glossinidia. The ancient coevolution of these species has led to the integration of Wigglesworthia metabolism into tsetse physiology and a subsequent reliance on nutrient exchange for maintaining the fitness of both partners. The establishment of this obligate mutualism has facilitated the emergence of feedback mechanisms that stabilize the symbiosis, such as that which involves the exchange of L-serine and B vitamins. The Gammaproteobacteria Wigglesworthia supplement B vitamins lacking …

Evaluating The Effects Of Modifying Mixing And Pelleting Parameters On Feed Quality, Pellet Production Rate, And Broiler Performance, Lucas E. Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to evaluate the effect of different mixer types (MTY) and mix times (MTI) on mix uniformity (MU). A 2 (mix time) x 3 (mixer type) factorial was utilized in this experiment to create six treatments, each mixed in three randomized complete blocks. Mix times were designated adequate or inadequate, were based on the manufacturers’ recommendation, and varied based on the mixer utilized. The mixer types utilized included a vertical mixer, horizontal mixer, and transfer mixing system. The transfer mixing system can be described as a batch-to-mix system …

Three Essays On Political Economy, Colin Michael Steitz

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first chapter studies the impact of conventions and multi-day events on
hotel demand in the Indianapolis metro area. Using hand-collected data on
sporting events and conventions, we study the impact on hotel metrics: ADR,
Occupancy, Daily Rate, and Revenue. We show that traditional sporting
events generate little impact when compared to multi-day conventions. We
show that mega-events such as major championship games generate significant
increases in hotel demand. We find large and statistically significant effects for
multi-day conventions on hotel demand with very little evidence of crowding
out. A single day of a multi-day convention brings in approximately $928,000 …

Conservative Anabaptists Descriptions Of Delay, Comfort In Connectedness, And Ease In Cultural Tension When Seeking And Responding To Healthcare, Matthew Donald Hottle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Conservative Anabaptists description of delay in health seeking behaviors, comfort in connectedness, and ease in cultural tension when seeking and responding to healthcare

Background: Conservative Anabaptists are a large ethnic religious population that continues to grow in the United States. This includes groups of Amish, Mennonites, Hutterites, and Brethren populations residing in rural areas. These groups are known to experience adverse health related outcomes such as community acquired infections, advanced cancer diagnosis, and undiagnosed chronic conditions such as hypertension, diabetes, and heart disease. Therefore, the concept transcending health values has been developed to better understand how conservative anabaptists describe …

Applications Of Digital Filters In Radio Astronomy, Joseph William Kania

Graduate Theses, Dissertations, and Problem Reports (ETD)

The radio sky spans tens of orders of magnitude in length, density, and time.
In this thesis, using novel filtering techniques and two different telescopes,
we investigate two tracers of cosmic structure: Baryon Acoustic Oscillations
(BAOs) and Fast Radio Bursts (FRBs). BAOs formed as the universe cooled
after the Big Bang. BAOs provide a fiducial length scale of the universe
throughout cosmic time and thus can be used to understand how the universe
is evolving. FRBs are very bright, short timescale, bursts of as-yet unknown
origin which occur uniformly on the sky at a rate of a few thousand per …

Understanding The Impact Of Scientific Testimony On Potential Jury Members: Independent And Interactive Effects Of Expert Characteristics And Jury Member’S Social Location, Ellory R. Dabbs

Graduate Theses, Dissertations, and Problem Reports (ETD)

As science and technology evolve, they impact society at different levels. With these changes, it is important to understand how members of society will be affected. One area where there has been a rise in the use of scientific information is in jury trials. Those who are on trial for a criminal offense are more often relying on forensic evidence, and forensic experts, to aid in their defense, and those who are prosecuting a criminal case are also relying on forensic evidence and forensic experts. However, forensic evidence is not consistent in type or interpretation – what one person may …

The Diversity And Synaptic Connectivity Of Somatostatin-Containing Inhibitory Interneurons In The Somatosensory (Barrel) Cortex, Rachel Elaine Hostetler

Graduate Theses, Dissertations, and Problem Reports (ETD)

The neocortex is the most evolutionarily advanced part of the mammalian brain and is responsible for a multitude of important tasks, such as sensory processing, movement, memory and learning and, in humans, cognition, and language. Within the neocortex, there are intricate circuits of neurons that are responsible for these tasks. These circuits are comprised of a delicate balance of excitatory and inhibitory neurons. Inhibitory interneurons have the crucial role of constraining and controlling the amount of excitation in the brain; disruptions in this balance can lead to a number of neuropsychiatric diseases and disorders. Our studies focused on the subpopulation …

Three Essays On Health And Environmental Economics: Applications Of Spatial Econometrics And Spatial Analysis, Mohammed Syedul Islam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Spatial interaction and the locational structure between observations play key roles in the field of econometrics for both cross-sectional and panel data analysis. Compared to a non-spatial econometric model, a spatial model relaxes the assumption of independency in observations. This research applies spatial and non-spatial econometrics in three different fields of applied economics: (1) drinking water and air quality violations impacts on lung and bronchus cancer incidence in the contiguous United States (U.S.); (2) spillover effects of non-pharmaceutical interventions (NPIs) on COVID-19 cases across the contiguous U.S. counties; and (3) urbanization impacts on carbon dioxide (CO₂) emissions in …

American Art Songs In The 21st Century: A Catalogue Of Selected Works, Islei Mariano Correa Hammer

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research explores American art songs of the 21st century. The paper provides a listing of 54 composers based in the United States and the art songs they composed from 2000 to 2023 which are available either through publishers or the composers’ websites. The research is divided into three categories: art songs with piano, chamber works with voice, and works for solo voice and large ensemble. The paper also includes biographies of each composer. This research provides singers, voice teachers, and collaborative pianists a useful resource to gain familiarity with the American art-song repertoire of the current century.

“I’Ll Tell You No Lies”: An Exploration Of Trauma, Memory, And Violence Against Women In North Carolina Murder Ballads, Madison Ava Helman

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores trauma, memory and violence against women in Western North Carolina murder ballads “Tom Dooley,” “Poor Omie Wise,” “Poor Ellen Smith,” “The Ballad of the Lawson Family,” and “Frankie Silver.” I posit that these ballads were influenced by prescriptive societal conceptions of femininity, which in turn influenced societal ideations of violence against women. Using folklore performance theory, I analyze the text and context of these ballads and their subsequent histories, eventually arriving at a template for polyvocality that incorporates multiple ballad variants and encourages diverse performances.

Evaluation Of Changes In Cellular Behavior Upon Exposure To Selected Analytes Used In Synthetic Applications, Olivia L. Rose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Metal-organic frameworks (MOFs) are organic crystalline hybrids that have been used for a variety of applications from catalysis to gas storage and drug delivery. Studies have shown that generally, while such frameworks are more biocompatible than similar aspect ratio nanostructures, they are also more prone to transformation and disintegration due to their flexible and fragile nature as resulted from the structure assembly and implemented structure-function relationships. With extensive previous literature showing that materials with nano dimensions such as carbon nanotubes, quantum dots and other nanomaterials have unique physicochemical properties that lead to increased biosystems’ reactivity. With increasing concerns regarding adverse …

“Ingenious Entanglements”: Sheriffs, Capitalism, And Civil Society In America, 1750-1850, Chad R. Holmes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sheriffs served as indispensable legal actors who facilitated the expansion of democracy and capitalist transformation, processes that eventually limited their ability to profit from their work and diminished their social status. This dissertation is organized around specific sheriffs and the actions they performed to illustrate how county officers influenced society beyond the execution of court orders. These men—Rhode Island’s Beriah Brown, Pennsylvania’s John Barker, and Massachusetts’s Elisha Porter, Luke Baldwin, and Charles P. Sumner—believed they understood how to balance community demands and state directives in ways that would produce personal profit and greater authority. The work of these officers and …

Abundance-Habitat Relationships And Aquatic-Terrestrial Habitat Selection Patterns For Wood Turtles (Glyptemys Insculpta) In The Upper Midwest, Jena Marie Staggs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Due to range-wide population declines, wood turtles (Glyptemys insculpta) are under review for federal listing under the United States Endangered Species Act. Understanding how habitat associations and environmental conditions influence population dynamics and spatiotemporal activity patterns is fundamental for designing effective management strategies. Previous work on wood turtle abundance-habitat relationships focused on broad-scale habitat relationships in northeastern North America. However, regional differences in climate, habitat conditions, and land use patterns limit their applicability to much of the wood turtle’s Upper Midwest distribution. Much of our understanding of wood turtle activity patterns is based on radiotelemetry studies, which are biased towards …

The Effect Of Pubertal Stress On Prefrontal Cortex Related Behavior In Adult And Adolescent Mice, Bryan Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

The prefrontal cortex (PFC) is an area of associated with cognitive flexibility, decision making, and value processing. The most common neuropsychiatric disorders globally, including generalized anxiety disorder, major depressive disorder, schizophrenia, and substance abuse disorders, have been associated with dysfunction in the PFC. One of the environmental factors that may lead to increased risk for developing these disorders is exposure to stressful or traumatic situations during key developmental periods, such as adolescence. Human studies found that individuals who were faced with early adverse events but had some form of social interaction or support during that time, had a lower risk …

Conditioned Reinforcement By Stimuli Correlated And Uncorrelated With A Schedule Of Food Reinforcement, Lillian L. Skiba-Thayer

Graduate Theses, Dissertations, and Problem Reports (ETD)

A conditioned reinforcer derives its function from a relation to an established reinforcer. Respondent views suggest that a stimulus acquires reinforcing properties because it is positively correlated with an established reinforcer. Alternatively, the information hypothesis suggests that a stimulus acquires reinforcing properties because it reduces uncertainty about the likelihood of an established reinforcer. Observing response procedures have been used to evaluate the function of stimuli positively correlated (S+), negatively correlated (S-), and uncorrelated (S1, S2) with established reinforcers such as food (with animals) or money (with humans). Verbal instructions have been shown to alter the extent to which correlated and …

Shear Bond Strength Comparison Of Bioceramics To Root Dentin., Yasmin Alma Hoffman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The purpose of this study is to compare the push-out bond strength of four bioceramic materials to root dentin that are used during regenerative endodontics. The bioceramics being tested are: 1. Biodentine®(Septodont); 2. EndoSequence BC Putty Fast Set® (Brasseler); 3. NeoPutty® (Avalon Biomed); and 4. ProRoot MTA White® (Dentsply). A high bond strength of a bioceramic material to root dentin will provide excellent seal. An improved seal may correlate with less potential for contamination of the pulp space that is undergoing regenerative activity. This in turn will lead to long-term clinical success. The null hypothesis is that there is …

Three Essays On Environmental And Energy Economics, Maher F. Mekky

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this dissertation, three related topics are investigated about environmental and energy economics. The research in these essays utilize panel data and regression models. The overall theme of these essays is to explore the relationships between energy and the environment in the United States (U.S.).

In the first essay, using data from fifty U.S. states between 2012 and 2020, the impacts of three types of state level policies on electric vehicles (EV) adoption are examined: 1) policies that mitigate the environmental impacts from energy production, 2) policies that provide financial incentives to consumers for EV purchase, and 3) policies that …

Navigating Place And Gender: A Multicontextual Critical Narrative Inquiry Of Rural Trans* Student Experiences, Jessie Lynn O'Quinn

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this critical narrative study was to understand how rural West Virginia trans* students navigate cultural norms of their rural home communities and higher education contexts. An essential part of this critical narrative was to provide rural trans* students with an avenue to share their unique experiences and give them a platform to share their voices. The resulting narratives suggested that the normative tensions rural trans* college students experience across contexts stemmed from negative regional experiences that reinforced traditional gender norms. Negative home contexts and experiences forced students to feel like they had to build walls and distance …

Another Covid Causality: Media Landscape In Bangladesh, Ershad Komal Khan

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study seeks to understand the pressure from both advertisers and the Bangladesh government on the local mass media between March 2020 and December 2021 concurrent with the impacts of the COVID-19 pandemic. Thus, this study aims to explore whether the local mass media compromised more with advertisers amid the COVID recession to earn advertising-based revenue and whether the Bangladesh government mounted more pressure on the press during the period as well as to analyze the influence of the aforementioned factors on newsrooms. To guide this work, this study employs the Market Theory of News Production (McManus, 1994), the Authoritarian …

The Economics Of Pollution: Policy, Productivity, And The Structure Of Road Networks, Michael Cary

Graduate Theses, Dissertations, and Problem Reports (ETD)

Air pollution impacts economic activity, and economic activity creates air pollution. Understanding this endogenous nexus is critical as any policy designed to affect one will inevitably affect the other. My research explores both sides of this nexus. Specifically, I identify the economic effects from the implementation of an international climate agreement (the Kyoto Protocol); I determine the impact of air pollution on physical productivity and determine which pollutants are responsible for this effect; and I link urban form to vehicular emissions via the structure of road networks.

The first chapter of this dissertation studies the impact of the Kyoto Protocol …

The Transmission Of Saga At West Virginia University, James Froemel

Graduate Theses, Dissertations, and Problem Reports (ETD)

Changes in the higher education landscape continue to bring new regulations which enforce standardization and similarities in organizational behavior. Because of these pressures, institutional identities and differentiations are more dependent on the unique way students experience an institution rather than the structure of the organization. Through focus groups and interviews, this phenomenological study seeks to understand the ways in which the traditional undergraduate experience impacts the transmission of organizational saga at West Virginia University. To do so, it examines stories told by students through thematic analysis to connect student experiences with symbols of the university. Focusing on these connections and …

Extension Of The Ergot Alkaloid Gene Cluster, Samantha Joy Fabian

Graduate Theses, Dissertations, and Problem Reports (ETD)

Specialized metabolites produced by fungi impact human health. A large portion of the pharmaceuticals currently on the market are derived from metabolites biosynthesized by microbes. Ergot alkaloids are a class of fungal metabolites that are important in the interactions of environmental fungi with insects and mammals and also are used in the production of pharmaceuticals. In animals, ergot alkaloids can act as partial agonists or antagonists at receptors for 5-hydroxytryptamine (serotonin), dopamine, and noradrenaline as ergot alkaloids have chemical structures similar to those neurotransmitters. Therefore, they affect insects and mammals that consume them and can be used to produce drugs …

Application Of Metagenomic And Molecular Microbiology Techniques To Elucidate Sources Of Fecal Pollution And Anthropogenic Antibiotic Resistance Genes To Surface Water: A Step Towards A “One Health” Approach To Watershed Management, Mehedi Hasan Tarek

Graduate Theses, Dissertations, and Problem Reports (ETD)

The use of fecal indicator bacteria, such as Escherichia coli, is a widely established regulatory and monitoring practice to detect surface water contamination associated with fecal pollution. However, the detection or quantification of fecal indicator bacteria alone does not accurately inform the sources of fecal pollution. The development of molecular and metagenomic methods that target the DNA of microorganisms has resulted in a host of new tools for monitoring fecal pollution and its sources, as well as for understanding emerging microbial threats, such as antimicrobial resistance. Antimicrobial resistance is a critical “One Health” challenge presenting a high risk to …

Beneficial Impacts Of Nod-Like Receptor X1(Nlrx1) Agonism In Lipopolysaccharide-Induced Inflammation, Mena Mansy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: The regulation of inflammation at the cellular and molecular level is a crucial aspect of homeostasis that is often overlooked, and most treatment options are limited to antibiotics and symptom management drugs. The development of novel molecular-targeted therapy options downstream of pathogen recognition receptors will fulfill the unmet need for therapy to effectively regulate excessive inflammation. NLRX1 is a unique NOD-like receptor that is anti-inflammatory in nature; it has been shown to negatively regulate the NF-kB pathway and has been involved in the pathology of a plethora of inflammatory disorders. This makes NLRX1 an emerging therapeutic molecular target for …