Digital Commons Network^™

“Situation Is Out Of Control:” Drug Use, Violence, Resistance, And Politics During The Vietnam War, Andrew Domenic Ray

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Vietnam War was notable because it was a very violent and very public war. Throughout the course of the conflict, the United States imposed anti-guerilla warfare on a grand scale. American soldiers were often given orders to destroy the enemy at all costs; but in a war with no discernable front lines, the difference between combatant and non-combatant was, at best, blurry. The way the war was being fought, coupled with its unpopularity and emerging counterculture at home, made the Vietnam War a hub for illicit drug use among American soldiers. American soldiers use drugs for a number of …

The Effect Of Alternative Work Regimes On Recruiter Productivity, Keith Sean Cogdell

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation examines the impact of different work regimes, onsite, remote, and hybrid, on recruiter productivity within the staffing industry. As workforce models evolve, organizations encounter challenges in determining how these structural changes influence employee performance. Existing studies are often cross-sectional and rely heavily on self-reported data, limiting their capacity to capture long-term trends and contextual factors. To address this issue, the present research utilizes a longitudinal design with five years of recruiter-level performance data from a national staffing firm, which includes over 27,000 observations. Using Self-Determination Theory (SDT) as a framework, the study investigates how intrinsic motivation mediated by …

Parental Behavioral Control Across Domains In Indian And Indian American Families, Avneet Batra

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prior research literature has often found parental behavioral control to have mixed associations with varied adolescent outcomes (Bean et al., 2006; Finkenauer et al., 2005). However, a relatively lesser explored topic of research focusses on the domain specificity of this kind of control and its association with internalizing and externalizing behaviors in sample of Indian and Indian American parents (Arim et al., 2010). The current study adds to the limited literature by emphasizing the five domains of control (e.g., moral, conventional, prudential, personal and overlapping) based on the social cognitive domain theory and explores their association with adolescent adjustment outcomes …

Monitoring West Virginia Wastewater For Norovirus: A Test Case For Tracking Foodborne Illness Outbreaks In Rural Communities Using Wastewater, Haley M. Phillippi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Foodborne illnesses are a global public health challenge. Foodborne illnesses, like norovirus, often have a large disease and societal burden. Norovirus is a single-stranded, positive-sense RNA virus, with 10 identified genogroups (GI-GX). Genogroups GI and GII are responsible for nearly all human cases of acute gastroenteritis worldwide. In the United States, HuNoV is not notifiable case-by-case. Instead, outbreaks are reported to national surveillance systems when two or more cases are epidemiologically linked. Most norovirus outbreaks occur during Winter to early Spring, however, certain individuals may still shed and transmit the virus without being detected or reported. Wastewater monitoring is a …

Revitalizing The Urban Riverfront: Redesigning The West End Bridge In Pittsburgh,Pa For Sustainable Multi-Use Connectivity And Public Space Creation., Mina Kargari Rezapour

Graduate Theses, Dissertations, and Problem Reports (ETD)

Urban waterfronts are critical assets for cities, offering opportunities for ecological restoration, public engagement, and multi-modal transportation. However, many riverfronts remain underutilized due to infrastructure barriers and lack of accessibility.

Pittsburgh’s riverfront is dynamic asset that supports recreation, cultural expression, and community interaction. The Three River Heritage Trail Loop, a visionary project led by River Life over the past 20 years, seeks to connect diverse neighborhoods and natural assets along the city’s rivers. At the heart of this initiative is the West End Bridge, serving as the bookend and a pivotal gateway to complete the loop and critical link for …

Evaluation And Identification Of Novel Viruses In Wild Fishes Using Varied Molecular Biology Techniques, Clayton Douglas Raines

Graduate Theses, Dissertations, and Problem Reports (ETD)

Viruses are among the most abundant biological entities on Earth, yet their diversity and ecological roles in aquatic vertebrates, particularly fishes, remain poorly characterized. This dissertation advances fish virology through integrative approaches that combine historical context, molecular diagnostics, and genomic discovery. A novel hepadnavirus (ApHBV) was identified in asymptomatic Alosa pseudoharengus, emphasizing the prevalence of subclinical infections in migratory forage species. In Catostomus commersonii, transcriptomic profiling revealed sexually dimorphic immune responses to hepatitis B and C-like viruses, highlighting complex host-pathogen dynamics. In Micropterus nigricans, next-generation sequencing uncovered a novel nackednavirus and multiple adomaviruses within hyperpigmented lesions, expanding …

The Dna Methylation Capacity Of The Tsetse Fly: Glossina Morsitans, And Its Integration With Symbiont Nutrient Provisioning, Dylan Forrest Richmond

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tsetse flies (Diptera: Glossinidae) are the vector of trypanosomes (Trypanosoma spp.), which are responsible for African trypanosomiasis, also known as sleeping sickness. African trypanosomiasis is a significant health and economic burden yet remains a neglected tropical disease. As an obligate blood feeder, tsetse flies are reliant on B vitamin supplementation from intracellular Gammaproteobacteria, Wigglesworthia glossinidia. Folate (B9) is essential for tsetse development and reproduction, as well as DNA methylation in animals. As a reversible modification, DNA methylation typically occurs in a CpG dinucleotide context and can alter gene expression via the restriction of transcription. Across insects, the …

The Impact Of Skew On The Flexural Behavior Of Press-Brake-Formed Tub Girders, Matthew Allan Weatherholt

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Short Span Steel Bridge Alliance (SSSBA) is a group of bridge and buried soil steel structure industry leaders who have joined together to provide educational information on the design and construction of short span steel bridges in installations up to 140 feet in length. Press-brake-formed-tub-girders (PBFTGs) were developed by a technical working group within the SSSBA in response to a rising demand for rapid infrastructure replacements for short span bridge applications. PBFTGs are produced from structural steel plate and can be finished as weathering steel or galvanized. After being cold bent to the appropriate shape, shear studs are welded …

Deep Target Recognition: Semi-Supervised Annotation, Sensor Fusion And Super-Resolution, Shoaib Meraj Sami

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite the recent expansion of machine learning algorithms to cover a wide range of disciplines, several areas of automatic target recognition (ATR) remain underexplored. This dissertation presents tools developed to improve performance in three significant aspects of ATR: semi-supervised annotation, sensor fusion, and image super-resolution. The aim of the semi-supervised methods is to automatically annotate targets in scenarios where labeled data are scarce in the target domain but available in the source domain. Secondly, to address the limitations of individual image sensors and enhance robustness under different environmental conditions and man-made constraints, a sensor fusion algorithm was developed to improve …

Replicating The Functionality Of Ghost Knifefish Cerebellar Feedback Using Synthetic Nervous Systems, Sheldon Paul Cj Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory inputs allow animals to perceive, react, and adapt to an environment. However, the sensory information received by the body, such as visual, auditory, and proprioceptive information, could become overwhelming, thus overloading the brain. Yet, animals can process all this information by canceling redundant signals from their surroundings, allowing them to be more sensitive to novel or unexpected signals in their environment. Each species (i.e., birds, fish, mammals) has its own way of using and filtering sensory information, from auditory to locomotion adaptivity. Cerebellar circuits contribute to sensory filtering in a variety of systems. In particular, research on Ghost knifefish …

Ciliary Arl14b In Vision, Alexis Breann Crockett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary cilia are highly specialized organelles that regulate signaling, trafficking, and sensory perception across multiple tissues, including the retina. The small GTPase ADP Ribosylation Factor-Like Protein 13B (ARL13B) is an atypical ARF-family protein enriched in cilia. ARL13B acts as a guanine exchange factor (GEF) for another small GTPase ARF-like Protein 3 (ARL3). Mutations in ARL13B cause Joubert syndrome, a ciliopathy characterized by brain malformation, developmental delays, and retinal degeneration. Despite ARL13B’s known ciliary localization and clinical relevance, ARL13B’s precise functions in cilia remain poorly defined. This dissertation investigates the cilia-specific roles and GEF-specific roles of ARL13B in photoreceptors using two …

Exploring The Ruminal Microbiome And Its Impacts On Feed Efficiency In Beef Cattle, Taylor Simone Sidney

Graduate Theses, Dissertations, and Problem Reports (ETD)

The ability for beef cattle to utilize feed efficiently is a critical determinant for both economic and environmental sustainability in livestock production. However, despite extensive research, the complex microbial ecosystem within the rumen and its relationship to feed efficiency remain incompletely understood. This dissertation explores the physiological and microbial foundations of feed efficiency by characterizing how rumen microbial communities and their functions differ among beef cattle with divergent residual feed intake (RFI) phenotypes and how the RFI status of beef cattle influenced their response to dietary interventions such as supplementation with a Bacillus-based feed additive. In the first study, …

Sleep, Endocannabinoid Signaling, And Cortical Processing In Mouse Models Of Autism Spectrum Disorder, Bryan Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sleep plays a crucial role in memory consolidation, neuronal plasticity, and cognitive function. Individuals with autism spectrum disorder (ASD) often experience sleep disturbances, which are positively correlated with symptom severity. However, on the surface, sleep architecture is remarkably normal under baseline conditions in many rodent models of ASD. The goals if this dissertation were to investigate whether regulation of sleep, biochemical signaling during sleep, and downstream consequences of sleep on behavior are altered in two mouse models of ASD. In Aim 1, we tested the hypothesis that sleep phenotypes emerge in ASD models under conditions of high sleep pressure. We …

Feasibility Testing Of A Web-Based Phq-9 Test For Student Depression, Israel Oluwagbenga Odugboye

Graduate Theses, Dissertations, and Problem Reports (ETD)

Feasibility Testing of a Web-Based PHQ-9 Test for Student Depression

Israel Odugboye

Student depression stands as a critical public health issue because it affects numerous young adults who experience academic decline, disability, absenteeism, productivity reduction, and suicidal thoughts. The general population shows a 10% to 15% lifetime risk of developing depression which leads to a twentyfold elevation in suicide risk for depressed individuals [3]. The Patient Health Questionnaire-9 (PHQ-9) serves as a popular tool for detecting depressive symptoms and determining severity levels among students from different cultural backgrounds. The assessment contains 27 possible scores which follow DSM depressive symptom criteria …

Investigating The Psychological Determinants Of Rural Volunteer Firefighters’ Health, Occupational Performance, And Resilience, Candice Jaye Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

Volunteer firefighters (VFFs) are essential to rural emergency response, and their ability to perform effectively depends on their mental and physical readiness. Given the high prevalence of mental and physical health challenges among VFFs, there is a critical need for research to better understand how these factors interact to develop targeted interventions that can systematically improve VFF functioning. This study addressed gaps in the literature by examining the intersections and mechanisms shaping rural VFFs’ coping functions, physical health, occupational performance, resilience, and social support. An explanatory sequential mixed methods design was employed, beginning with a quantitative survey of 180 West …

Bridging The Gap Between Structural And Cellular Biology: Insight Into The Organization Of Glycolytic Enzymes In Cells, Heather Hansen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glycolytic enzymes are spatially organized in the cytoplasm, and are shown to localize with cellular structures such as the plasma membrane, F-actin, mitochondria, or associate independently within the cytosol. The assembly of these enzymes is hypothesized to regulate pathway flux to meet the energetic needs of the cell. However, it is unclear whether all of these assemblies have the same mechanism of formation or function in the cell. We investigated the role of glycolytic enzyme assemblies in cells, using the glycolytic “gatekeeper” enzyme phosphofructokinase-1 (PFK1). PFK1 is extensively regulated by multiple mechanisms, including over 20 allosteric ligands, post translational modifications, …

Weakly Reversible Deficiency Zero Realizations Of Polynomial Dynamical Systems, Neal Ryan Buxton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Weakly reversible, deficiency zero (WR0) systems form a large class of polynomial ODEs modeling chemical reaction networks. Their behavior is exceptionally stable: they have unique positive steady states, which are locally asymptotically stable (they are also conjectured to be globally asymptotically stable). This powerful result (the Deficiency Zero Theorem) applies to a large class of high dimensional, nonlinear polynomial dynamics, and is independent of the choice of parameters in the model. In this dissertation we present two algorithms that expands the scope of the Deficiency Zero Theorem to:

1. Networks with WR0 realizations, i.e. networks that are not necessarily WR0 …

Probing The 10-23 Dnazyme Catalytic Core: Insights From Structural And Mechanistic Studies, Evan Rhyse Cramer

Graduate Theses, Dissertations, and Problem Reports (ETD)

The 10-23 DNAzyme is a catalytically active DNA oligonucleotide capable of cleaving RNA at purine-pyrimidine junctions in the presence of divalent metal ions, most notably magnesium. Despite its promise as a programmable RNA knockdown tool, its clinical and biotechnical utility has been hampered by limited structural characterization, low catalytic efficiency under physiological conditions, and poor understanding of its in-cell activity. This dissertation presents a comprehensive framework for the structural and functional analysis of DNAzymes using the 10-23 DNAzyme as a model system. In the absence of high-resolution structures, we first employed detailed in vitro biochemical assays to map essential residues …

A Durability Evaluation Of Class B Concrete Bridge Substructures Under West Virginia Freeze–Thaw Conditions, Nathaniel Wayne Dunbar

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study evaluates whether the West Virginia Division of Highways (WVDOH) Class B concrete specification for bridge substructures remains adequate under modern durability and design-life expectations. The current specification, which permits a minimum compressive strength of 3,000 psi, reflects practices established when shorter service lives were generally assumed. Modern AASHTO LRFD provisions are based on a 75-year design life, raising concern that this lower-strength concrete may not provide sufficient durability under West Virginia’s climatic conditions.

To address this issue, bridge inspection data, regional climate mapping, and durability modeling were used to assess the condition and performance of concrete substructures across …

Parameter Estimation Of A 100 Seater Simulated Passenger Aircraft, Michael James Winston

Graduate Theses, Dissertations, and Problem Reports (ETD)

Virtual flight test aircraft provide good opportunities to test new parameter estimation methods. However, good estimates for the parameters in VIRTTAC Castor, a virtual test aircraft, are unavailable. This thesis uses two well-established time domain methods, the Output Error Method and the Filter Error Method, to estimate the parameters of VIRTTAC Castor. Three standard maneuvers were used: Elevator 3-2-1-1, Rudder Doublet, and Aileron Multi-Step. The parameters were estimated across three different flight conditions: weight, altitude and air speed, which resulted in 27 tests for each maneuver. All parameters, and standard deviations estimated are included in the appendix as a table.

Effects Of Environmental And Anthropogenic Gradients On Fish Beta Diversity In Central Appalachian Streams, Alanna Kaylee Lowther Smith

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding the environmental and spatial drivers of community composition is essential for conserving freshwater biodiversity. We examined how environmental gradients, land cover, and spatial structure influence fish beta diversity in wadeable streams across West Virginia, USA. We quantified total beta diversity and partitioned it into species replacement and richness difference components and used local contributions to beta diversity (LCBD) to identify compositionally unique sites. Community–environment relationships were evaluated with distance-based redundancy analysis (dbRDA), and a beta regression GLMM was used to test how elevation, slope, land cover, and stream order (factor) predicted LCBD while accounting for spatial structure (dbMEMs) and …

Identification, Mapping And Treatment Of The Invasive Rosa Multiflora Using Unmanned Aerial Systems And Machine Learning Models, Kylie Virginia Shaw

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species are introduced species that cause detrimental impacts to the native ecosystem or cause harm to human health. The work necessary for land managers to survey for and treat invasive species takes up limited time, labor, and financial resources. This study assesses how Unmanned Aerial Systems (UAS) and machine learning models can be used to make the identification, mapping, and treatment of invasive vegetation more efficient and effective. A UAS-mounted multispectral camera captured images of our target species, Rosa multiflora (multiflora rose), throughout its growing season in southwest Pennsylvania. A random forest machine learning model was trained on these …

Implementation Of A Neural Network Execution Framework For Generalized And Cross-Platform Deep Learning Deployment And Inference On Spacecraft Systems, Rafael Polanco Segovia

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent advances in hardware and software technology have made it possible to implement more resource-demanding deep learning algorithms in constrained hardware environments. This creates opportunities to use deep learning for aerospace applications on increasingly smaller aerospace vehicles. This work presents the implementation of a Neural Network Execution Framework (NNEF), which aims to provide a cross-platform and reusable framework to deploy and execute trained neural networks for deep learning aerospace applications. The NNEF executes any neural network inference process regardless of the original deep learning framework in which it was created, for supported flight software platforms, and space-like computer boards. Users …

Green Bank Chime/Frb Outriggers Commissioning And Analog System Development, Kholoud Sharif Tag Alkhatem Khairy

Graduate Theses, Dissertations, and Problem Reports (ETD)

The main objective of this thesis is to document the development and commissioning of the Canadian Hydrogen Intensity Mapping Experiment. (CHIME) outrigger at the Green Bank Observatory. (GBO) in Green Bank, WV. This novel cylindrical wide-field radio transient telescope is currently operating in conjunction with CHIME. The CHIME outrigger at GBO aims to contribute significantly to the field of radio astronomy, with implications for both Fast Radio Burst. (FRB) science and broader astronomical research. The construction and commissioning of the CHIME outrigger at the GBO mark a pivotal step forward in pursuing high-precision wide-field detection and localization of radio transients, …

Watershed-Level Survey Of Antimicrobial Resistance From Onsite Wastewater Systems, Trey Franklin Clark

Graduate Theses, Dissertations, and Problem Reports (ETD)

The emergence of antimicrobial resistance (AMR) has quickly become one of the largest global health and development threats. Research related to environmental modes of transmission of AMR has accelerated in recent years, and the US Environmental Protection Agency is particularly interested in this emerging contaminant. Knowledge of how onsite (decentralized) wastewater treatment systems contribute to this issue is lacking, so this research serves to analyze and quantify the environmental dissemination of AMR and fecal contamination from these sources at a watershed level while using statistical analysis to assess relationships between physiochemical, spatial, and sampling parameters. This research surveyed four watersheds …

A Learning History Of A State Physical Activity Plan, Jessica Nicole Riffee

Graduate Theses, Dissertations, and Problem Reports (ETD)

Physical inactivity remains a persistent public health concern in the United States, disproportionately affecting rural populations like West Virginia. To address statewide physical activity challenges, West Virginia developed one of the nation’s first state level physical activity plans, ActiveWV: 2015, that was revised to ActiveWV: 2030. Limited research has investigated how statewide physical activity plans unfold over time or how stakeholder learning can drive improvement.

This dissertation employed a learning history action research methodology to construct a collective narrative of the development, dissemination, implementation, and evaluation activities across both iterations of the West Virginia Physical Activity Plan. Reflective interviews with …

Development Of Novel Carbohydrate-Based Nanoparticles For Precision Drug Delivery, Krystal Ann Hughes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoparticle-based drug delivery has made tremendous advancements in the last decade. However, several critical factors, including precision drug delivery at the site of interest, long-term storage stability, and controlling release profiles of drugs in the systemic circulation, need to be addressed for successful clinical translation of nanomedicine. This dissertation presents novel carbohydrate-based nanoparticles that achieve precise intracellular delivery and therapeutic release at the disease site. We present the formulation and optimization of polymeric and polymer-lipid hybrid nanoparticles that enable precision intracellular delivery and passive accumulation of therapeutics in the bone marrow. Intracellular delivery is highly impactful and sought after. One …

The Professional Backgrounds And Experiences Of Higher Education Trustees And Its Influence On Decision Making, Jade J. Irving

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are many complex decisions that must be made among the board of governors within institutions of higher education. Recent headlines on the decisions of trustees regarding campus closures and consolidations, program cuts, and presidential appointments have put a spotlight on the individuals responsible for making such decisions. This study examined how trustees of public 4-year institutions in the state of West Virginia perceive the value of their professional backgrounds and experiences in fulfilling their governance responsibilities. Using an explanatory sequential design, this study investigated how trustees’ professional experiences shape their decision-making processes through the theoretical lenses of sensemaking theory …

Three Essays On Sustainability Economics, Hélène Flore Nguemgaing

Graduate Theses, Dissertations, and Problem Reports (ETD)

As global economies face increasing resource dependencies and supply chain challenges, it becomes essential to understand the vulnerabilities that threaten economic stability. This dissertation investigates three empirical issues in agribusiness and critical minerals using econometric models to estimate the following: 1) the impact of supply chain disruptions on stock market returns during COVID-19; 2) the extraction of rare earth elements from acid mine drainage treatment by investigating the impacts of ownership and legislation in West Virginia and Pennsylvania; 3) China-to�FOBb (Free-on-Board) price transmission in rare earth elements markets: a copula-based dynamic tail dependence analysis.

The first essay evaluates how events …

Machine Learning And Uass For Managing Autumn Olive (Elaeagnus Umbellata) On Reclaimed Surface Mines, Sean Michael Keane

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research introduces an innovative approach for managing autumn olive (Elaeagnus umbellata) on reclaimed surface mines through the integration of machine learning algorithms and unmanned aerial systems. The proliferation of autumn olive presents significant ecological challenges on disturbed landscapes, necessitating more efficient detection and control methods. This study evaluated Random Forest classification and deep learning approaches using the U-Net architecture for identifying autumn olive across four phenological stages using high-resolution imagery. The RGB U-Net model achieved F1-scores of 0.927, 0.912, and 0.871 for autumn olive detection during peak, late, and senescence stages respectively, outperforming both multispectral and vegetation …