Investigating The Interplay Of The Immune System, Skin Barrier, And Microbiome Following Dermal Exposure To Triclosan, Rachel Baur

Graduate Theses, Dissertations, and Problem Reports (ETD)

Triclosan is an antimicrobial chemical used in healthcare products that are applied to the skin. However, exposure to triclosan has been positively associated with food allergy, aeroallergy, and asthma severity in humans. In mice, dermal triclosan exposure, although not directly sensitizing, has been shown to augment the allergic response in a model of asthma. Furthermore, exposure to triclosan on mouse skin has been demonstrated to be immunomodulatory through the activity of thymic stromal lymphopoietin and S100 calcium-binding protein A8. The skin barrier and microbiome are known to interact with the immune system and disruptions in barrier integrity and the microbiome …

Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases, Tasneem A. Arsiwala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transcranial low-intensity focused ultrasound is a unique technology to modulate the integrity of tight endothelial junctions and transiently increase BBB/BTB permeability to enhance therapeutic delivery. Despite promising early studies, present literature lacks agreement on key experimental conditions, which restricts our knowledge and the technique's widespread translation. This dissertation first provides a critical review of the current gaps in knowledge regarding the universal use of LiFUS in preclinical and clinical use. We then identify key parameters for translational and predictable opening of the BBB using a 3T MRI coupled with a clinical device. Our investigation highlights that passive permeability of the …

Bacterial Contamination In Public Atac-Seq Data And Alignment-Free Detection Methods, Drake Michael Aesoph

Graduate Theses, Dissertations, and Problem Reports (ETD)

ATAC-seq is a new high-throughput sequencing technology for measuring chromatin accessibility within genomic samples. It can be used to discover new information about open regions, nucleosome positions, transcription factor binding sites, and DNA methylation. It is especially useful when combined with other next-generation sequencing techniques, such as RNA-seq. Unlike previous technologies, however, ATAC-seq is more sensitive to bacterial contamination, which is a well-known problem in cell cultures that can lead to incorrect experimental results. Previous studies have measured the contamination in public RNA-seq data and found that 5%- 10% of samples were contaminated. In this report, we investigate the prevalence …

The Business Of Behavior: Appalachian Teachers’ Experiences In Using Positive Behavior Interventions And Support, Erin Michele Coffield

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the lived experiences of Appalachian elementary and middle school teachers who have been identified as working in Positive Behavior Intervention and Support (PBIS) schools. This study used Lifeworld Theory to engage in discussion about the teachers’ individual experiences in using PBIS in their classrooms in relation to resilience frameworks and childhood trauma. The teachers’ individual experiences were analyzed using a Whole-Part-Whole analysis to bring the individual stories together to better understand how PBIS and childhood trauma are experienced in a specific Appalachian school. Discussion opens up through the participants' experiences about grit models in school, trauma their …

Standardized Postoperative Handoff Tool To Improve Handoff Quality, Haley Durkacz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Effective communication between healthcare providers is an essential element in the delivery of safe patient care. Loss of relevant information can occur during all phases of patient care but is most inclined to occur during the handoff from the operating room to the postoperative anesthesia care unit (PACU). Evidence has indicated that the transfer of patients by anesthesia providers remains informal and brief despite practice guidelines. When pertinent details are omitted during the anesthesia handoff there is a potential increase for medical errors, delayed treatment, and patient harm. The purpose of this project was to analyze the use of …

Predictions Of Produced Water Quality And Recycled Water Optimization For Spatially-Distributed Wells In Point Pleasant Formation, Armel Quentin Mbakop

Graduate Theses, Dissertations, and Problem Reports (ETD)

The treatment of produced water as a fracturing fluid is becoming an increasingly important aspect of water management surrounding the booming of the unconventional oil and gas industry. Two main problems facing the oil and gas industry are the availability of water for well drilling and completion and disposal of the produced water. Unconventional well drilling and completion in the Utica shale requires large amounts of water. The wastewater that results after production—containing high levels of organic and inorganic matter— is usually disposed of through deep well injection. A new approach reuses this produced water as part of subsequent fracturing …

Vacant To Vibrant: Transforming Vacant Lots In North Philadelphia, Olivia Anne Sinclair

Graduate Theses, Dissertations, and Problem Reports (ETD)

The City of Philadelphia has addressed the need to utilize vacant lots in the city’s comprehensive planning document, Philadelphia2035 (PCPC, 2010). The document encourages designers to use sustainable practices to reincorporate these abandoned lots back into the fabric of the city. Through partnerships with local organizations in Philadelphia, guidelines and suggestions were created to guide Philadelphians while they revive abandoned lot spaces. In the neighborhood of Nicetown-Tioga, Philadelphia, there is a large amount of vacant lots leaving the area blighted and without resilience. This study accepts Philadelphia’s invitation to use design strategies to be applied to vacant lots within the …

Deep Learning Detection In The Visible And Radio Spectrums, Greg Clancy Murray

Graduate Theses, Dissertations, and Problem Reports (ETD)

Deep learning models with convolutional neural networks are being used to solve some of the most difficult problems in computing today. Complicating factors to the use and development of deep learning models include lack of availability of large volumes of data, lack of problem specific samples, and the lack variations in the specific samples available. The costs to collect this data and to compute the models for the task of detection remains a inhibitory condition for all but the most well funded organizations. This thesis seeks to approach deep learning from a cost reduction and hybrid perspective — incorporating techniques …

The Most Common Vocal Fault In The Baritone Voice, Matthew Derek Cyphert

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research project is to create an approachable guide to avoiding the most common vocal fault found in the baritone singing voice. The specific fault being discussed has been coined the “baritone swallow.” This document will explore the history of the baritone singing voice along with information regarding vocal fach identification, an in-depth study of this common vocal fault frequently experienced by baritone singers, and a fault-specific guide to identifying and addressing the “baritone swallow.”

Development Of Computational Methods For Electronic Structural Characterization Of Strongly Correlated Materials: From Different Ab-Initio Perspectives, Uthpala K. Herath

Graduate Theses, Dissertations, and Problem Reports (ETD)

The electronic correlations in materials drive a variety of fascinating phenomena from magnetism to metal-to-insulator transitions (MIT), which are due to the coupling between electron spin, charge, ionic displacements, and orbital ordering. Although Density Functional Theory (DFT) successfully describes the electronic structure of weakly interacting material systems, being a static mean-field approach, it fails to predict the properties of Strongly Correlated Materials (SCM) that include transition and rare earth metals where there is a prominent electron localization as in the case of d and f orbitals due to the nature of their spatial confinement.

Dynamical Mean Field Theory (DMFT) is …

An Efficient Ar Model-Based Method For The Detection Of Forced Oscillations In Power Networks: Implementation And Analysis, Maria Waleska Suarez

Graduate Theses, Dissertations, and Problem Reports (ETD)

An active research topic is the detection of various oscillations that may lead to instability and potential disruption in the operation of a power network. Forced Oscillations (FOs) play a unique role in power system stability among various oscillations. They are perturbances that change the system’s state and are caused for many reasons, including but not limited to persistent load changes and oscillatory load or generation, fault, triplane, and other mechanical anomalies. These factors can hugely affect the power grid by either increasing or decreasing the amplitude, causing corrupt modes leading to blackouts, affecting the equipment involved, delivering poor power …

Representation Of Muslims In Time Magazine: A Corpus Linguistic Study, Constantia Rhinehart

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis sought to explore the representation of Muslims in US-American

Press. In order to do this, the Time Magazine Corpus was used to observe words that collocate with the word “Muslim” in Time Magazine. The frequency of the word “Muslim” was observed for each decade and coded to determine the semantic prosody of each token. The 1960s-2006 were observed in order to provide a more comprehensive view of Muslim representation in Western media and how this representation has changed over time. The majority of collocations were considered to be neutral, however, a large number of collocations were considered …

Red Spruce Ecological Sites, Ecological States, And Restoration Pathways Quantified Through Soil Organic Carbon, James Edward Leonard

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tools used by agencies and organizations like the Forest Service (FS), the Natural Resource Conservation Service (NRCS), the Nature Conservancy (TNC), and the Central Appalachian Spruce Restoration Initiative (CASRI) to help guide red spruce (Picea rubens) ecosystem restoration within Central Appalachia could better address outcomes from management practices implemented in terms of soil organic carbon (SOC) stock changes. These high-elevation landscapes have a natural capacity to produce diverse ecosystem services that affect humans, animals, and plants alike. Ecological site descriptions (ESD) are an important tool used to restore impacted landscapes and provide detailed management prescriptions specific to red spruce ecological …

Integration Of Spectroscopic And Mass Spectrometric Tools For The Analysis Of Novel Psychoactive Substances In Forensic And Toxicology Applications, Travon Cooman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Analytical methods aiming for the detection of novel psychoactive substances are continuously revised due to their utility in the seized drug and toxicology realms. One method frequently employed for the preliminary identification of illicit materials is portable Raman spectroscopy. Even when a substance in possession of an offender is identified, conclusive evidence that it may have been consumed requires additional confirmatory work and further toxicological evaluation of a biological specimen. Many times, the substance consumed may not be detected in the analyzed specimen due to its extensive metabolism. It is therefore challenging to rule out the identity of the drug …

Influence Of Saturation Nonequilibrium And Variable Operation Conditions On The Electromechanical Performance Of Ionic Polymer Metal Composite Actuator Architectures, Allison Maria Arnold

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electroactive polymers (EAPs) continue to gain attention for their potential to offer unique and versatile solutions in the soft robotic and flexible electronic industries. Ionic Polymer-Metal Composites (IPMCs) are a class of ionic-type EAPs which can be configured as capacitor actuators with very low voltage requirements (≤ 5 V AC or DC). Their compact, portable, and lightweight properties, coupled with a biomimetic bending actuation response make them ideal for human-machine integrated technologies such as medical implants, active skins, and artificial muscles. The Nafion-based IPMC can be described as a layered composite capacitor containing an ionic polymer core, sandwiched between chemically …

Population Characteristics Of Three Black Bass Species In The Upper Ohio River Drainage, Levi Foster Brown

Graduate Theses, Dissertations, and Problem Reports (ETD)

In West Virginia, species of black bass are economically and ecologically important and support popular fisheries. In the state’s large, navigable river systems, recent information suggests that anglers consider these fisheries diminished relative to their historic stocks. This study sought to describe the population characteristics of Largemouth, Smallmouth, and Spotted Bass in the upper Ohio River drainage and evaluate possible management actions. Low relative abundances coupled with poor growth estimates for Largemouth and Spotted Bass suggests that a suite of environmental and anthropogenic factors may be reducing the quality of their recreational fisheries. Conversely, demographic estimates produced for Smallmouth Bass …

Synthesizing Realistic Substitute Data For A Law Enforcement Database Using A Python Library, Anthony Carrola

Graduate Theses, Dissertations, and Problem Reports (ETD)

In many databases, there is private or sensitive data that should not be accessible to any but a few individuals, such as HIPAA (Health Insurance Portability and Accountability Act) protected or LE (law enforcement) data. However, there is often a need to work with the data or change it for proper and thorough testing, especially for the developers . In some cases, the developers may be authorized to access and view the data, but it is rarely allowable for that data to be changed. Further, it is unlikely, especially on a large project, that all of the developers will have …

Anthropogenic Disturbance Regimes Impact The Microbial And Chemical Composition Of Soils And Sediments Across Ecosystems, Gregory D. Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Human activities have led to profound changes in the climate and environment, fundamentally changing global carbon and nitrogen cycles. Ongoing conversion of wildland for human use and the intensification of agricultural systems have dire consequences for biodiversity and the ecosystem services that support society. While natural disturbance regimes are important for the long-term maintenance of native biodiversity and ecosystem function, many disturbances are becoming increasingly frequent due to anthropogenic activities. Increasing the frequency, intensity, or duration of disturbances can prevent ecosystems from returning to their previous equilibria. Disturbance-induced changes to the local environment shape microbial communities, which in turn govern …

Geotextile Design And Performance For Dewatering Of Rare Earth Element Sludge Produced From Acid Mine Drainage, Cory J. Nasiadka

Graduate Theses, Dissertations, and Problem Reports (ETD)

Acid Mine Drainage (AMD) is an environmental legacy of mining operations that forms when sulfide minerals within the earth are exposed to water and oxygen and has significant harmful effects on the environment when left untreated. Researchers at West Virginia University found large concentrations of REE/CMs (Rare Earth Elements and Critical Minerals) in AMD treatment sludge. REE/CM consist of a group of 17 elements that are crucial to the production of advanced technologies. Due to an extremely low domestic supply of REE/CM in the United States, domestic sources and production of REE/CM are being investigated. This research investigates geotextile design …

Narrative Inquiry Of Early Career Teachers’ Experiences Teaching During The Covid-19 Pandemic, Kinsey Ann Wright

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study utilized narrative inquiry to learn the stories of early career teachers of students with disabilities as they navigated teaching during a global pandemic. Five early career teachers participated in a series of three semi-structured virtual interviews. Interviews were transcribed then analyzed using a six-phase approach to thematic analysis (Braun & Clarke, 2006). Nine themes were identified: Teaching, Communication, Parents, Special Education, Workload, Resources, Training and professional development, job satisfaction, and teacher preparation. Themes were organized and presented to answer the research questions of the study. The findings contribute to understanding pandemic-related challenges faced by early career teachers of …

Similitude, Scaling, And Prediction Of Stream Dissolved Oxygen Across The Contiguous U.S., Aron Gebreslase

Graduate Theses, Dissertations, and Problem Reports (ETD)

Dissolved oxygen (DO) is a general indicator of stream water quality and ecosystem health. However, the concentration of in-stream DO is controlled by various climatic, land use/cover, hydrologic, biochemical, and ecological drivers. Observational data for stream DO are often unavailable at the desired temporal and spatial scales. Accurate and reliable prediction of stream DO concentration based on a small set of environmental drivers is important to guide the water managers and policymakers to achieve and maintain healthy streams. This dissertation focused on identifying the dominant controls of stream DO and understanding the mechanisms and environmental regimes by employing machine learning, …

Polychromatic Colorings Of Certain Subgraphs Of Complete Graphs And Maximum Densities Of Substructures Of A Hypercube, Ryan Tyler Hansen

Graduate Theses, Dissertations, and Problem Reports (ETD)

If G is a graph and H is a set of subgraphs of G, an edge-coloring of G is H-polychromatic if every graph from H gets all colors present in G on its edges. The H-polychromatic number of G, poly_HG, is the largest number of colors in an H-polychromatic coloring. We determine poly_HG exactly when G is a complete graph on n vertices, q a fixed nonnegative integer, and H is the family of one of: all matchings spanning n-q vertices, all 2-regular graphs spanning at least n-q vertices, or all cycles of length precisely n-q. …

Experience Of Parents Caring For A Child With An Acquired Disability, Brad E. Phillips

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Parents caring for a child with an acquired disability often report a negative impact on physical, psychological, emotional, and spiritual health associated with caregiver burden related to their child’s complex health needs. This is further complicated by additional challenges related to the loss of a previously healthy child. Traditional patterns of loss follow linear, time-bound stages that have been observed to be unrelated to the experiences of these specific parents and their families. Therefore, the concept transforming loss has been developed to better understand how these parents navigate loss and find meaning in spite of their suffering.

Purpose: The …

Fasciotomies, Gabriel Bass

Graduate Theses, Dissertations, and Problem Reports (ETD)

This collection of poems follows the disjointed and never complete trajectory of personal recovery from trauma and alcoholism. The poems and their arrangement trouble the notion of a person ever being fixed, even as they make progress toward a better version of themselves. The collection illustrates how the past can impose itself on the present, creating dissonance and complicating the speaker’s understanding of time and recovery.

New Techniques In Celestial Mechanics, Ali Abdulrasool Abdulhussein

Graduate Theses, Dissertations, and Problem Reports (ETD)

It is shown that for the classical system of the N body problem ( Newtonian Motion), if the motion of the N particles starts from a planar initial motion at t=t_{0}, then the motion of the N particles continues to be planar for every t\in[t_{0},t_{1}], assuming that no collisions occur between the N particles. Same argument is shown about the linear motion, namely, for the classical system of the N body problem, if the motion of the N particles starts from a linear initial motion at t=t_{0}, then the motion of the N particles continues to be linear for every …

The Largest Linear Subspace Contained In Darboux-Likr Functions On R, Gbrel Mohammad Albkwre

Graduate Theses, Dissertations, and Problem Reports (ETD)

Consider an arbitrary $\mathcal F\subset\mathbb R^\mathbb R$, where the family $\mathbb R^\mathbb R$ of all functions from $\mathbb R$ to $\mathbb R$ is considered as a linear space over $\mathbb R$. Does $\mathcal F\cup\{0\}$ contain a non-trivial lineal subspace? If so, how big the vector space can be? These questions are at the core of lineability theory. In particular, we say that a family $\mathcal F\subset\mathbb R^\mathbb R$ is {\em lineable (in $\mathbb R^\mathbb R$)}\/ provided there exists an infinite dimensional linear space contained in $\mathcal F\cup\{0\}$.

There has been a lot of attention devoted to lineability problem of subsets …

Novel Modes Of Synchronization And Extreme Events In Coupled Chemical Oscillators, David Allen Mersing

Graduate Theses, Dissertations, and Problem Reports (ETD)

We experimentally and computationally investigate dynamical behaviors in coupled chemical oscillators. These networks of chemical oscillators are created using catalytic Ru(bpy)₃²⁺ loaded cation exchange beads submerged in catalyst-free Belousov-Zhabotinsky (BZ) solutions. Various network structures are created by utilizing the photosensitive nature of the Ru(bpy) ₃²⁺ catalyst. The response of the oscillators due to light stimuli can be characterized by constructing a phase response curve (PRC). The PRC quantifies the excitatory and inhibitory responses of BZ oscillators due to applied light perturbations as a function of the oscillators' phase. Different initial concentrations of reactants in the BZ reaction …

The Lived Experiences Of Selected Choral Directors During The Covid-19 Pandemic, Angela Berna Milliren

Graduate Theses, Dissertations, and Problem Reports (ETD)

The shift to online education during the COVID-19 pandemic found secondary choral teachers moving traditional performance-based courses to the online venue. The pedagogical changes needed include implementing technology and disseminating information through learning management systems. Relationships between teachers and students, and teachers and colleagues, were challenged with the physical distance of quarantines in the Spring of 2020. This dissertation research project examined the difficulties six teachers faced concerning technology and relationships. I sat down for semi-structured interviews with six colleagues where I asked about their backgrounds, relationships with students and fellow colleagues, and the changes the pandemic brought to their …

Electronic Cigarette Dependence: User And Device Characteristic Predictors, Ashley E. Douglas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Understanding the dependence potential of electronic cigarettes (ECIGs) is critical to informing prevention and regulatory efforts. Extant work on ECIG dependence is limited, in part due to the vast number of products that differ on their ability to deliver nicotine. Further, existing research is based largely on former/current cigarette smokers, and thus confounds dependence on nicotine via ECIGs versus cigarettes. This study evaluated ECIG device/liquid characteristics and use behaviors as predictors of ECIG dependence in a sample of experienced ECIG users who never smoked cigarettes. Participants completed an online survey that assessed ECIG device/liquid features, use behavior, and ECIG dependence. …

Regional Correlation And Depositional History Using Well Log And Core Data Of The Geneseo-Burket From The Poseidon 8m Well, Westmoreland County Pa, Usa, Spencer Leonard Williams Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Regional Correlation and Depositional History using Well Log and Core Data of the Geneseo-Burket from the Poseidon 8M Well, Westmoreland County PA, USA

Spencer Williams

Natural gas producers have invested billions of dollars in Pennsylvania and West Virginia to establish significant gas production from the Devonian Marcellus Shale and the deeper Ordovician Utica-Point Pleasant interval. In addition, commercial gas production has been reported from several other Devonian shale units in the Appalachian region, including the Rhinestreet, Levanna, and Geneseo-Burket. The Marcellus Shale is the largest natural gas play in the United States. The Marcellus is located directly under the …