Digital Commons Network^™

Estimating The Age Of A Bloodstain Using Mitochondrial Rrna, Jordan R. Flesher

Graduate Theses, Dissertations, and Problem Reports (ETD)

DNA evidence is considered the gold standard of evidence in forensic investigations. DNA is one of the most highly discriminatory pieces of evidence found at crime scenes and can link individuals to scenes. It is not relied on to perform temporal estimates to help establish a timeline for when a crime took place. Previous studies have shown that measurement of the degradation of RNA is a potential tool to establish a temporal estimate of when a blood sample was left at a crime scene. This experiment attempts to improve on previous RNA degradation studies by evaluating the validity of mitochondrial …

The Museum Of Queer Curiosities, Reba "Feliks" Kay Pyron

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper documents the research, personal motivations, and processes that make The Museum of Queer Curiosities (TMOQC). The installation, exhibited in the Paul Mesaros Gallery, uses the manipulation of traditional techniques in printmaking, sculpture, and craft, to create a space in which authority, labels, confusion, and compassion are discussed. Through the synthesis of familiar visual art installation techniques and museum display, the work attempts to provide a foothold for the viewer to navigate queerness and ephemeral identity. The body of work that occupies TMOQC creates the surreal simulation of a scientific museum, transforming the art gallery into a stage where …

Respectable Women, Ambitious Men: Gender And Family Networks In Victorian Sheffield, Autumn Mayle

Graduate Theses, Dissertations, and Problem Reports (ETD)

“Respectable Women, Ambitious Men: Gender and Family Networks in Victorian Sheffield” offers a family study of Nonconformist manufacturers in nineteenth-century Sheffield through several thematic case studies on such subjects as gender, family networks and businesses, bankruptcy, piety, and charitable work. This project focuses on the Reads, a family of middle-class Congregationalist smelters who owned a smelting works, named Read & Co., in nineteenth-century Sheffield. The Reads’ experiences contribute to recent scholarship on gender and kinship studies by addressing the role of nineteenth-century conceptions of masculinity and femininity on family, charity, and business and the enduring influence of nuclear families and …

Estimating Evolutionary Volatility In A Maximum-Likelihood Framework, Samuel Warren Tybout

Graduate Theses, Dissertations, and Problem Reports (ETD)

Evolutionary volatility is a trait that encompasses a clade’s combined capacity for origination and extinction. High volatility increases extinction risk, and declining global extinction rates are thought to be linked to declining volatility. Despite volatility’s scientific importance, there is no standardized way of measuring it. This study provides a new method, derived from a stochastic birth-death model, of estimating evolutionary volatility from fossil data. Simulations indicate that the method produces accurate and precise estimates for large fossil datasets. Analysis of fossil data for five bivalve families (Lucinidae, Mytilidae, Pectinidae, Pholadomyidae, and Veneridae) indicates that diversity projections made from the estimates …

Nutrient Cycling-Tree Species Relationships In Appalachian Forests, Philip Michael Crim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Since the colonization of North America by Europeans, ecosystems in Appalachia and across the continent have been in a prolonged state of flux. Areas particularly rich in natural resources, such as Appalachia, have historically borne the brunt of these swift changes, often with devastating consequences. Downwind of much of the power generation facilities of the Ohio Valley, Appalachian forests have been geographically predisposed to high rates of acidic deposition, a circumstance mitigated by the passage of Clean Air Legislation beginning in the 1970s. Nevertheless, decades of elevated nitrogen (N) and sulfur (S) inputs had a profound impact on the ecology …

The Morphological, Physiological, And Genetic Underpinnings Of Intraspecific Salinity Tolerance In Sorghum Bicolor, Ashley N. Henderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Decreases in land quality and quantity threaten the efficient production of agriculturally and economically pivotal crops. Such reductions in arable lands are a consequence of population growth and urbanization, and often result in the introduction of various abiotic stresses. The most common abiotic stressors include water limitation (drought), water logging (over watering), poor water quality (salinity), and extreme temperatures (cold, frost, heat). Each of these stressors negatively impact plant growth, development, and yield. Soil salinity, specifically, is a considerable constraint affecting lands used in agriculture. Salts in the soil rise both naturally and through anthropogenic factors making the abundance a …

Developmental Mechanisms For The Diversification Of Polyphenic Morphs In The Head Horn Of Onthophagine Beetles (Coleoptera: Scarabaeidae Onthophagus Taurus): Plasticity Through Nutrition, Logan Paul Zeigler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Developmental plasticity is the phenotypic variation between organisms that is caused by environmental interactions affecting the developmental systems of organisms. The research focused primarily on nutrition-responsive developmental plasticity. In this research we used the nutritionally determined head horn development of Onthophagus taurus to better understand the developmental mechanisms and genetic underpinnings of nutrition-responsive trait development. We focused specifically on altering the availability of specific nutrition-related primary metabolites, cholesterol and palmitic acid, identified in the activity of The Hedgehog pathway, a critical pathway in head horn development. By altering diet composition using cholesterol, reducing transcript expression of an acyltransferase gene, rasp …

Leaf Angle And Leaf Stomata Responses To Experimental Drought In Quercus Velutina And Acer Saccharum, Brittany Nichole Casey

Graduate Theses, Dissertations, and Problem Reports (ETD)

The possibility of increased severity and frequency of drought conditions, as a result of global climate variability, greatly complicates our ability to forecast future forest functions such as productivity and carbon sequestration. Assessing how tree species vary in their response to drought can aid in predicting the impact on forest ecosystems as a whole. Throughfall exclusion (TfE) experiments are potentially useful tools to simulate realistic drought conditions within intact forest ecosystems. We employed a TfE experiment during the 2018 growing season within the WV Land Trust’s Elizabeth’s Woods Nature Preserve, near Morgantown, WV, to assess the leaf angle and leaf …

Predictors Of Self-Control During Emerging Adulthood: The Roles Of Implicit Beliefs And Early Risk, Katy L. Delong

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study explored how early adverse experiences (i.e., low socioeconomic status, household chaos, attachment insecurity) and implicit beliefs about self-control (i.e., whether self-control is a limited or nonlimited resource) were associated with trait and momentary self-control in a sample of college students. As the first study to explore these factors together, individuals’ implicit beliefs were tested as a moderator and meditator of the association between early risk and self-control. Participants (N = 214) first completed a baseline survey with the main predictors and trait self-control, followed by one week of experience sampling to assess momentary self-control, or success resisting …

Blind Image Denoising Using Supervised And Unsupervised Learning, Surekha Pachipulusu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Image denoising is an important problem in image processing and computer vision. In real-world applications, denoising is often a pre-processing step (so-called low-level vision task) before image segmentation, object detection, and recognition at higher levels. Traditional image denoising algorithms often make idealistic assumptions with the noise (e.g., additive white Gaussian or Poisson). However, the noise in the real-world images such as high-ISO photos and microscopic fluorescence images are more complex. Accordingly, the performance of those traditional approaches degrades rapidly on real-world data. Such blind image denoising has remained an open problem in the literature.

In this project, we report two …

Hard Work Beats Talent, Unless Talent Works Hard: Exploring The Relationship Between Self-Efficacy And Sales Performance, Mohammad Sakif Amin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Current research in sales suggests that salesperson self-efficacy, which is a person’s belief about his/her ability to achieve a desired level of performance in a given task, affects sales performance positively. The logic behind this positive effect is that people who exhibit a high level of self-efficacy have increased motivation and thus exert increased efforts towards completing a task. Research in psychology, however, suggests that there can be a downside to self-efficacy. Drawing on perceptual control and ego depletion theories, this dissertation explores two possible dark side effects of self-efficacy in a sales context: (1) decreased salesperson effort engendered by …

The Immunological Response To Chronic Stress In Obese Mice, Matthew Corey Parsley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity and psychological stress are major risk factors for cardiovascular disease and have a positive correlation with vascular dysfunction. This functional deficit has been linked to a pro-inflammatory milieu, exacerbated by an upregulation of pro-inflammatory cytokines. Febuxostat, a xanthine oxidase inhibitor, has been shown to rescue vascular impairment in these models. The purpose of this study was to examine the individual and combined effects of obesity and chronic stress on neuroimmune cell populations, and assess if febuxostat is able mediate immune alterations.

Male mice (n=48) were assigned into either normal chow (lean) and high-fat diet (obese) groups. The lean animals …

Elucidating A Common Mechanism Of Proteasome Impairment In Neurodegenerative Disease And Its Pharmacological Intervention, Tiffany Ann Thibaudeau

Graduate Theses, Dissertations, and Problem Reports (ETD)

Proteostasis is maintained by several systems in the cell including the ubiquitin-proteasome system (UPS), chaperones, chaperone-mediated autophagy, and macroautophagy. The UPS is the principle route for the degradation of intracellular misfolded, damaged, or unneeded cellular proteins and has a critical role essential every cell process, including: cell cycle progression, transcriptional regulation, genome integrity, apoptosis, immune responses, and neuronal plasticity. When the efficiency of protein degradation is perturbed, misfolded and damaged protein aggregates can accumulate to toxic levels and cause neuronal dysfunction, which may underlie many neurodegenerative diseases. It is widely appreciated that soluble oligomers of misfolded proteins (e.g. Aβ, Alzheimer’s; …

Analysis Of Unconventional Oil And Gas Impacts On Downstream Fish Assemblages And Physiological Stress, Joshua Noah Ankeny

Graduate Theses, Dissertations, and Problem Reports (ETD)

Unconventional Oil and Gas (UOG) production has been steadily expanding throughout the mid-Atlantic since 2008. Increased sedimentation, degraded water chemistry and an overall decrease in habitat quality due to UOG is anticipated to negatively impact aquatic inhabitants, a common observation in other stressed landscapes (i.e., mining, agriculture, development). We assessed stream health through both community analysis and physiological parameters (i.e., growth and hematocrit). A before-after-control-impact study uncovered three fish metrics that were significantly different following UOG disturbances. The invertivore-piscivore metric decreased following UOG disturbance (p = 0.045) whereas two benthic metrics saw a surprising increase (p = 0.003 and p …

The Structural Information Filtered Features Potential For Machine Learning Calculations Of Energies And Forces Of Atomic Systems., Jorge Arturo Hernandez Zeledon

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the last ten years, machine learning potentials have been successfully applied to the study of crystals, and molecules. However, more complex materials like clusters, macro-molecules, and glasses are out reach of current methods. The input of any machine learning system is a tensor of features (the most universal type are rank 1 tensors or vectors of features), the quality of any machine learning system is directly related to how well the feature space describes the original physical system. So far, the feature engineering process for machine learning potentials can not describe complex material. The current methods are highly inefficient …

Mindfulness For Musicians: Bringing Sport Psychology And Mindfulness-Based Therapies To The Practice Room And The Concert Stage, Lauretta M. Werner

Graduate Theses, Dissertations, and Problem Reports (ETD)

Due to the profession’s competitive nature, time-consuming demands, and frequent evaluations, many musicians experience debilitating music performance anxiety, hypercritical thoughts, and/or avoidance of specific performance situations. To cope with these experiences, musicians can cultivate mindfulness. Mindfulness is purposefully and non-judgmentally paying attention to the present moment, developing awareness of emotions, thoughts, and physical sensations, and acknowledging the reality of one’s experience. “Mindfulness for Musicians: Bringing sport psychology and mindfulness-based therapies to the practice room and the concert stage” discusses how cultivating mindfulness helps musicians cope with intense emotions, such as panic or fear, and explores how cultivating mindfulness helps musicians …

Optimization Of Engineered Super Donor Strains Of Cryphonectria Parasitica To Reduce Canker Expansion In A Forest Setting, Amy Michelle Metheny

Graduate Theses, Dissertations, and Problem Reports (ETD)

Chestnut blight, caused by the non-native fungus Cryphonectria parasitica, has decimated American chestnut (Castanea dentata) populations throughout the eastern U.S. over the last century. Biological control of blight, termed hypovirulence, has emerged naturally in the form of a debilitating hypovirus transmitted through hyphal anastomosis among vegetatively compatible C. parasitica strains. Six unlinked vegetative incompatibility (vic) loci regulate hyphal anastomosis and thus, hypovirus transmission, which has limited biocontrol efficacy in genotypically diverse communities. Recently, four of six vic loci have been disrupted to engineer two “super donor” or SD strains. In combining these strains, enhanced transmission …

Elucidating Disease Dynamics In The Biocontrol Of Ailanthus Altissima While Confirming The Host Specificity Of The Vascular Wilt Pathogen Verticillium Nonalfalfae, Kristen L. Wickert

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ailanthus altissima is a highly invasive exotic tree species entrenched throughout the United States. Since the discovery of a vascular wilt disease of Ailanthus in 2002, caused by the fungus Verticillium nonalfalfae, many studies are investigating its potential as a biocontrol focusing on efficacy, host specificity and disease transmission. This study addresses: the reproductive potential of Ailanthus; if V. dahliae alters Verticillium wilt progression; if hardiness zones influence the individual contributions of two Verticillium spp.; can a unique habitat affect biocontrol efficacy; and can the fungal proteome of V. nonalfalfae allow us to explain differences in host …

Adolescent Residential Mobility: Behavioral Outcomes And The Moderating Role Of The Mother-Adolescent Relationship, Sloane B. Glover

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prior research has noted unfavorable associations between residential mobility (RM) and youth outcomes. However, little work has considered the mother-adolescent relationship as a moderator of the suggested associations. The purpose of the current study was to examine the internalizing, externalizing, and delinquent behaviors of adolescents (M_age=15.6, SD=0.77) following a move in comparison to adolescents who did not move. Frequency of moves was considered and adolescents were identified as either stable (0 moves), low mobility (1-2 moves), or high mobility (>2 moves) within a 6 year period. Mother-adolescent closeness and parental monitoring were examined as potential …

Aptamer Functionalized Zinc Oxide Field Effect Transistors For Odor Detection, Michael D. Aldridge

Graduate Theses, Dissertations, and Problem Reports (ETD)

Odor detection and identification are complex processes, and tasks that currently only animals do well. There is a pressing need for an electronic nose, or eNose, with good sensitivity, selectivity, and speed that mimics that ability. Food quality control operations, environmental sensing, occupational safety, and the defense sectors all require systems that can rapidly and reliably detect trace levels of volatile organic compounds. The goal of this work is to create a biologically inspired device which can accurately detect and identify odors at concentrations consistent with the most sensitive biological systems.

In order to mimic a natural olfactory system, we …

An Assessment Of Farmer Participation In The United States Department Of Agriculture, Natural Resources Conservation Services’ Conservation Technical Assistance Program In West Virginia, Matt D. Oliver

Graduate Theses, Dissertations, and Problem Reports (ETD)

Food and fiber production on America’s farmlands have a major influence on the environment, therefore, soil and water conservation practices are critical. NRCS has provided no-fee technical assistance for nearly 100 years through the Conservation Technical Assistance (CTA) program. The CTA program is essential because it provides technical knowledge directly to farmers for planning and implementing conservation practices that are proven to benefit environmental health and on-farm production. CTA program funds support NRCS staff and training and are thereby the local service delivery vehicle for all NRCS programs. However, in recent years, funding for CTA has remained relatively constant while …

Characterization Of Nodule-Specific, Slac And Mate Membrane Transporters In Medicago Truncatula, Lina Yang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Legumes play a crucial role in sustainable agriculture because of their intrinsic ability to reduce atmospheric N₂ into NH₃ via symbiotic nitrogen fixation (SNF). SNF is carried out in the symbiosome, a quasi-organelle containing endosymbiotic rhizobial bacteria in the cytoplasm of infected cells of the nodule. Therefore, the bacteria are surrounded by a symbiosome membrane (SM), which is derived from the plant plasma membrane during infection. SNF requires constant nutritional exchanges between symbionts, including reduced carbon (dicarboxylates) from the plant for reduced nitrogen (NH₄⁺) from the bacteroids. This exchange of nutrients and signals is fundamental …

Soil Health Assessment In Reclaimed Mine Soils, Katie Danielle Stutler

Graduate Theses, Dissertations, and Problem Reports (ETD)

West Virginia is one of the top coal-producing states in the nation, which has resulted in over 500,000 acres of highly disturbed, anthropogenic mine soils caused by surface mining. Mine soils are often rocky and contain low organic matter content, low plant-available nutrients, and toxic metals, which could result in poor soil health and low productivity. Soil health has been defined as the continued capacity of soil to function as a living ecosystem that sustains plants, animals, and humans. It is assumed that with time since reclamation, mine soil properties and related functions may change, and soil health will improve …

Dispositional Empathy And Autonomic Reactivity During A Comfort Interaction, Alaina G. Tiani

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of the current study was to examine the association between dispositional (trait) empathy and autonomic reactivity during dyadic interactions among women enrolled in undergraduate psychology courses. The primary research question was: do individuals who are deemed higher in dispositional empathy according to self-report exhibit differential autonomic reactivity patterns during a task in which they are asked to comfort another student experiencing emotional distress than those who are deemed lower in dispositional empathy? The literature on this relation has been mixed and has utilized photos or videos to evoke emotion; thus this study sought to examine the relation between …

A Biomimetic Approach To Controlling Restorative Robotics, Matthew T. Boots

Graduate Theses, Dissertations, and Problem Reports (ETD)

Movement is the only way a person can interact with the world around them. When trauma to the neuromuscular systems disrupts the control of movement, quality of life suffers. To restore limb functionality, active robotic interventions and/or rehabilitation are required. Unfortunately, the primary obstacle in a person’s recovery is the limited robustness of the human-machine interfaces. Current systems rely on control approaches that rely on the person to learn how the system works instead of the system being more intuitive and working with the person naturally. My research goal is to design intuitive control mechanisms based on biological processes termed …

Metal Nanomaterials: Immune Effects And Implications Of Physicochemical Properties On Sensitization, Elicitation, And Augmentation Of Allergic Disease, Katherine Adair Roach

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many allergenic metals are being manufactured in nanoparticulate forms. Although the decreased size profile of metal nanomaterials has been consistently associated with more pronounced lung toxicity compared to larger materials, it is unclear if metal-induced immunotoxic effects exhibit a similar size-dependency.

The central hypothesis of these studies was that metal nanoparticles cause more pronounced immunomodulatory effects on allergic processes than larger forms of respective metals. Moreover, it was anticipated that, similar to their inflammatory potential in airways, the magnitude of these responses would correlate best with the dose metric of surface area.

The first set of studies utilized fine and …

Musical And Cultural Perspectives Of Héctor Campos Parsi: An Examination Of His Influences And Analysis Of Selected Vocal Works, Cynthia Ortiz-Bartley

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper examines the historical and cultural atmosphere in Puerto Rico and how it affected one of Puerto Rico’s most celebrated composers, Héctor Campos Parsi, and two selected works in two different times of his life. The discussion includes events that affected the island with respect to art music from 1898, the birth of Héctor Campos Parsi in 1922, and up to 1986, which marks the date of the second selected work. This document considers three questions about cultural and structural influences about the life and experiences of Héctor Campos Parsi: Does a composer’s life experience and environment and culture …

The Role Of Communication Accommodation In Mother-In-Law/Daughter-In-Law Relationships, Erin Claire Shelton

Graduate Theses, Dissertations, and Problem Reports (ETD)

The goal of this thesis was to further the literature on mother-in-law/daughter-in-law relationships by directly employing Communication Accommodation Theory (CAT) to examine relationships between categories of accommodation and relational quality in these relationships. Daughters-in-law (N= 677) were solicited via private Facebook groups to complete an online survey regarding accommodation (their own and their mothers-in-law’s), their feelings of shared family identity, and their relationship satisfaction in their relationship with their mothers-in-law. Results demonstrated relationships between mothers-in-law’s accommodation, overaccommodation, and underaccommodation of their daughters-in-law and daughters-in-law’s feelings of shared family identify and relationship satisfaction. Additionally, relationships were present between daughters-in-law’s …

A Critical Assessment On The Resources And Extraction Of Rare Earth Elements From Acid Mine Drainage, Christopher R. Vass

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rare earth elements (REEs) are crucial to many modern products used in both civilian and defense applications. Currently, a reliable supply of these elements is uncertain with the clear majority of REE production and refining occurring predominately in China. Furthermore, domestic ore deposits with commercially attractive concentrations of REEs are uncommon in the United States. As a result, the identification of a domestic supply of these technology metals is essential not only for manufacturing consumer merchandise but also for national security. Recently, one promising source of REEs has been identified: coal and coal-byproducts. One of those is acid mine drainage …

Crown-Level Mapping Of Tree Species And Health From Remote Sensing Of Rural And Urban Forests, Fang Fang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tree species composition and health are key attributes for rural and urban forest biodiversity, and ecosystem services preservation. Remote sensing has facilitated extraordinary advances in estimating and mapping tree species composition and health. Yet previous sensors and algorithms were largely unable to resolve individual tree crowns and discriminate tree species or health classes at this essential spatial scale due to the low image spectral and spatial resolution. However, current available very high spatial resolution (VHR) remote sensing data can begin to resolve individual tree crowns and measure their spectral and structural qualities with unprecedented precision. Moreover, various machine learning algorithms …