Digital Commons Network^™

Improving The Biological Control Of Persicaria Perfoliata (Polygonaceae) Using Rhinoncomimus Latipes Korotyaev (Coleoptera: Curculionidae), Jaewon Kim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Persicaria perfoliata (L.) H. Gross (Polygonaceae; Mile-a-minute weed) is a rapid-growing invasive vine introduced from eastern Asia to northeastern United States in the 1930s. This vine has been invaded in disturbed areas and reforestation sites in 15 states in the U.S. and forms dense, monocultural patches that may inhibit natural forest regeneration. To control this weed, a host-specific biocontrol agent, Rhinoncomimus latipes Korotyaev (Coleoptera: Curculionidae) has been released in the P. perfoliata invaded states in the U.S. during the past 15 years. Currently, R. latipes is released by hand to the invaded area where the presence of the weed is …

Understanding Academic Relevancy: Linking Practice To Classroom Through Adventure Stem Education, Alice Morgan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research paper was to examine the relationship between vocational relevancy and adventure STEM education in the context of a four-day adventure STEM camp. Middle school students participated in a variety of adventure activities designed to teach STEM concepts. Using a concurrent triangulation mixed methods design, 246 students completed pre- and post-surveys that assessed changes in attitudes related to STEM interest, career interest, STEM career knowledge, STEM identity, STEM self-efficacy, and STEM value. Fourteen students and eight teachers participated in semi-structured interviews to further examine the role of vocational relevancy in curricula. Overall, results indicate some support …

Using Ai And Machine Learning To Indicate Shale Anisotropy And Assist In Completions Design, Cole E. Palmer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Operating companies in the unconventional Marcellus shale play have all faced a similar and problematic issue, while attempting to produce natural gas over the last decade. Companies have quickly realized that not every perforation along their horizontal wells are producing gas. In fact, producing perforations are only ranging from 15%-70% of the total perforations along the horizontal wellbore [1]. This unexplained issue results in millions of dollars in lost revenue per well, in addition to the sunk cost of paying for completions that are not actually yielding any produced gas.

What is causing these perforations to have no produced gas? …

Structural Transformations Of Synaptic Partners During Growth And Competition Of The Giant Nerve Terminal, The Calyx Of Held, In The Medial Nucleus Of The Trapezoid Body In The Developing Postnatal Mouse, Dakota Richard Jackson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Across many parts of the nervous system, initial neural circuit assembly involves stereotyped stages of early axonal proliferation within target territories, selective reinforcement of a subset of inputs and the removal of exuberant or unneeded connections via either activity or experience-dependent mechanisms, in a process referred to as synaptic competition. The mechanisms and associated structural transformations that underlie and define these stages of neurodevelopment have been examined in a variety of brain regions including the peripheral neuromuscular junction, the central climbing fiber innervation of cerebellar Purkinje cells and retinal ganglion cell innervation of the thalamic lateral geniculate nucleus. Each of …

Genetic And Biochemical Characterization Of Ergot Alkaloid Synthesizing Fungi And Their Symbionts, Matthew Duane Maust

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ergot alkaloids are fungal tryptophan derived toxins which affect mammalian circulation and neurotransmission. These compounds are biosynthesized by a conserved genetic pathway, known as the ergot alkaloid synthesis (EAS) pathway by fungi belonging to the ascomycete families Trichocomaceae and Clavicipitaceae. Several Ipomoea species and related plants in the morning glory family harbor vertically transmitted symbiotic fungi in the genus Periglandula, also members of Clavicipitaceae, that produce ergot alkaloids. Metabolomic analysis of seeds identified a previously uncharacterized glycoside form of the pharmaceutically important ergot alkaloid, ergonovine. Several species belonging to the fungal genus Metarhizium have recently been shown to have the …

Microbial Responses To Unconventional Oil And Gas Development May Alter Ecosystem Function In Headwater Streams, Rachel Michaels

Graduate Theses, Dissertations, and Problem Reports (ETD)

The demand for natural gas, and the need for efficient extraction, has led to the development of unconventional oil and natural gas (UOG) techniques. Due to the novelty of UOG, the potential impacts to freshwater ecosystems are not fully understood. We used a dual pronged approach to study the effects of UOG development on microbial biodiversity and function via a laboratory microcosm experiment and a survey study of streams with and without UOG development within their watersheds. The laboratory microcosm study simulated stream contamination with produced water, a byproduct of UOG operation, using sediment collected from one high water-quality stream …

Paleoecological Successions From Shallow-Marine Depositional Environments In Upper Silurian Carbonate Rocks Of Blair County, Pennsylvania, Shadya El-Ashkar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Studies of paleoenvironment and paleoecology are important for investigating relationships between the different environmental and biological variables that influenced biodiversification patterns during the Silurian Period. The mixed carbonate and siliciclastic rocks of the Bloomsburg and Mifflintown Formations in central Pennsylvania represent transitions between carbonate ramp and deltaic settings. The goal of this study was to assess changes in depositional environment and paleoecology of an undescribed Bloomsburg/Mifflintown locality in Bellwood, Pennsylvania. In central Pennsylvania, the interfingered relationship of mixed carbonate and siliciclastic mudstones of the Mifflintown Formation with red fine-grained sandstones and shales of the Bloomsburg Formation presented challenges for deciphering …

Fundamental Studies On The Recovery Of Rare Earth Elements From Acid Mine Drainage, Xue Huang

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Rare Earth Elements (REEs) are a group of 17 chemically similar elements that are essential raw materials for modern society. Given their increasing importance in civil and defense applications, many national governments, including the US, have denoted the REEs as critical materials and emphasized the need to identify and develop both conventional and unconventional resources to meet the growing demand. To address this challenge, the US Department of Energy has recently spearheaded efforts to evaluate the technical and economic feasibility of recovering REEs from coal and coal byproducts, which have been shown to contain REEs at an elevated concentration …

Cobalt-Catalyzed Aerobic Oxidative Cyclization Reactions Of Bisnucleophiles: New Methodologies And The Role Of Bisnucleophiles In O2 Activation, Jiaqi Liu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the past few decades, transition metals have found wide applications in the development of selective oxidative transformations mediated by molecular oxygen. Due to the benign nature of molecular oxygen as an oxidant and an increasing awareness of green chemistry practice, tremendous progress has been made towards the development of Cu-and Pd-catalyzed aerobic oxidation reactions. As a first-row transition metal alternative to copper, cobalt has been employed in aerobic catalytic transformations for its cost-efficiency and earth abundance; however, redox-active mediators such as benzoquinone (BQ), N-hydroxyphthalimide (NHPI) or salen-type ligands are usually required. To date, reactions mediated by Co/O₂ catalytic …

Minocycline As A Treatment For Traumatic Brain Injury-Induced Impulsive And Attentional Deficits, Kristen M. Pechacek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Traumatic brain injury (TBI) impacts millions worldwide and can cause lasting psychiatric symptoms. Chronic neuroinflammation is a characteristic of post-injury pathology and is positively associated with psychiatric conditions such as ADHD and bipolar disorder. Therefore, the current study sought to treat TBI-induced impulsivity and inattention using minocycline, an antibiotic with anti-inflammatory properties. Rats were trained on the five-choice serial reaction time task (5CSRT), a measure of motor impulsivity and attention. After behavior was stable on the 5CSRT, rats received either a bilateral frontal TBI or sham procedure. Minocycline was given at either 1 h post-injury or beginning at 8 weeks …

Evaluation Of Fitts’ Throughput As A Possible Performance Measure For Manual Assembly Tasks, Pradyumna Bettagere Jagadish

Graduate Theses, Dissertations, and Problem Reports (ETD)

Assembly lines are the backbone of the manufacturing sector. Workers at the assembly lines are expected to perform fast pace hand-arm exertions with precision and accuracy. To protect the workers from injuries, OSHA mandates the use of personal protective equipment (PPE) including gloves. Certain assembly operations require the use of special gloves. Some of these gloves can inhibit hand performance, further increasing the task difficulty. The Index of Difficulty (ID) is a metric measured in ‘bits’ quantifies task difficulty through the quotient of target width and target motion, i.e., distance travelled. The time required to complete the target motion is …

Factors Influencing Huntingtin Aggregation At Surfaces: Implications For Huntington’S Disease, Sharon E. Groover

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington’s Disease (HD) is a genetic, neurodegenerative disease characterized by an abnormal polyglutamine (polyQ) expansion in the first exon of the huntingtin protein (htt). The polyQ domain facilitates aggregation and initiates the formation of a diverse collection of aggregate species, including fibrils, oligomers and annular aggregates. The first 17 amino acids of htt (Nt17) directly flank the polyQ domain and is a key factor in htt’s association to membranous structures. In addition to Nt17 being an amphipathic αhelix, it also promotes aggregation through self-association and contains numerous posttranslational modifications (PTMs) that can modulate toxicity and subcellular localization. For in depth …

Synthesis And Characterization Of Pyridine Dipyrrolide Iron Complexes Relevant To Nitrene And Carbene Group Transfer, Brett M. Hakey

Graduate Theses, Dissertations, and Problem Reports (ETD)

The synthesis and electronic structures of the square-planar high-spin Fe^II complexes (^MesPDP^Ph)Fe(OEt₂) and (^MesPDP^Ph)Fe(thf) were investigated. Analysis of these compounds using a combination of ¹H NMR and ⁵⁷Fe Mössbauer spectroscopies, in conjunction with computational analysis, has resulted in a rationalization for their unusual electronic structures, which arise from the unique coordination environment enforced by the pyridine dipyrrolide chelate ligand.

The reactivity of (^MesPDP^Ph)Fe(thf) with organic azides has been probed and resulted in the isolation of an array of products that are consistent with …

Cerebrovascular Dysfunction And Degeneration In Alzheimer’S Disease Pathophysiology, Dominic Dimitri Quintana

Graduate Theses, Dissertations, and Problem Reports (ETD)

Alzheimer’s disease (AD) is a terminal illness and the most common form of dementia, which disproportionately affects the aged population. The pathophysiology of AD is characterized by neurodegeneration that slowly progresses, affecting regions of the brain that are involved in learning, memory, language, and executive function. In patients with the disease, early symptoms include non-disruptive forgetfulness that evolves into the inability to form new memories and ultimately the loss of autonomy at late stages. Histopathological hallmarks in the brain from patients with AD is the presence of amyloid-β (Aβ)-plaques and neurofibrillary tangles (NFT) deposited in the parenchyma. Since the discovery …

Avian And Salamander Response To Young Forest Management In West Virginia, Eric L. Margenau

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation focuses on the effects of various young forest habitat management techniques on the avian and salamander community in West Virginia. Wildlife species associated with the nascent stage of forest succession are experiencing precipitous population declines throughout much of the eastern United States due to decreases in the amount of young forest area which have been brought on by changes in disturbance regimes over the past century. As a result, the need to find novel approaches for creating young forest habitats to sustain young forest wildlife populations is necessary. However, young forest habitat creation often negatively affects species that …

Alluvial Fans, Loess Plains, Lakes, And Distributive Fluvial Systems: Depositional Systems Of The Permian-Triassic Red Beds And Evaporites Of Wyoming (Usa), Jonathan Knapp

Graduate Theses, Dissertations, and Problem Reports (ETD)

The end-Permian ecological crisis was a period of warming and aridity reaching extreme levels by the end Permian and not recovering before the Middle Triassic. The Permian Goose Egg Formation and the Chugwater Group’s Early Triassic Red Peak Formation and Middle Triassic Alcova Limestone are a continuous or nearly continuous succession of rocks deposited during this warm, arid time in Wyoming. Comprised of red beds, evaporites, and rare carbonates, these rocks have not been subjected to detailed sedimentological investigations in the modern era. Previous interpretations of marine and marginal marine deposition have largely been based on the laterally extensive nature …

Evaluating The Use Of The M-Vac® Wet Vacuum System To Recover Dna From Cotton Fabric, Phillip Reilly Irion

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the current state of the field of forensic biology, collecting DNA from evidence samples may pose some difficulty. Traditional methods for collection of DNA from biological fluids and touch samples include cuttings, scrapings, and the double swab method. While all of these methods have benefits, they also have their drawbacks. In order to more efficiently collect DNA from an evidence sample, novel approaches need to be explored. This novel approach is the microbial vacuum (M-Vac®) Wet Vacuum System.

This system utilizes a sterile collection solution and a vacuum to collect DNA material from evidence samples. While it is similar …

Effect Of Western Diet And Caloric Substitution With Apple Pomace On Fatty Acid Composition And Gene Expression In The Hypothalamus Of Growing Female Sprague-Dawley Rats, Ayad Abdulhamid Alawadi

Graduate Theses, Dissertations, and Problem Reports (ETD)

The hypothalamus plays a major role in regulating food-intake and energy-expenditure. Hypothalamic dysfunction implicated in neurodegenerative diseases is characterized by diet-induced neuroinflammation and energy dysregulation. Apple pomace, a waste byproduct of processing, is rich in polyphenols and soluble fibers, has the potential to ameliorate diet-inducing inflammation. The objective of this study was to determine the effects of Western diet and caloric substitution of Western diet with 10% apple pomace on brain fatty acid composition and gene expression related to hypothalamic function and health in growing female rats. Growing female Sprague-Dawley rats (age 22-29 days) were randomly assigned (n=8 rats/group) to …

Spawning Characteristics Of Yellow Perch During Periods Of Water Level Fluctuations In A Hydropower Reservoir, Kyle J. Matt

Graduate Theses, Dissertations, and Problem Reports (ETD)

Water level fluctuations alter reservoir ecosystems causing direct and indirect effects on fish populations. The dewatering of eggs, a direct impact of lake level drawdowns, can affect reproductive success of species that spawn in littoral zones, such as Yellow Perch. I examined relationships between water level fluctuations and spawning characteristics of Yellow Perch in a Central Appalachian hydropower reservoir, where water levels were permitted to be drawn down to 4 m and 2.1 m below the full pool elevation in March and April, respectively. Daily presences of egg masses were recorded on artificial spawning structures at two sites for the …

Protein/Peptide Characterization Using Mass Spectrometry And Molecular Dynamics Simulations, Ahmad Kiani Karanji

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mass spectrometry (MS) based-techniques and molecular dynamics (MD) simulations have been used to characterize protein/peptide structure as well as their interactions with lipid vesicles and detergents. Chapter 1 introduces an introduction to the concepts and tools that were used in this work. In Chapter 2, the dominant gas-phase conformer of [M+3H]3+ ions of the model peptide Acetyl-PSSSSKSSSSKSSSSKSSSSK are examined with ion mobility spectrometry (IMS), gas-phase hydrogen deuterium exchange (HDX), and mass spectrometry (MS) techniques. This section furthers the development of a protein structural prediction tool by providing information about gas-phase ion conformers of two model peptides having different solution conformational …

Palmprint Gender Classification Using Deep Learning Methods, Minou Khayami

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gender identification is an important technique that can improve the performance of authentication systems by reducing searching space and speeding up the matching process. Several biometric traits have been used to ascertain human gender. Among them, the human palmprint possesses several discriminating features such as principal-lines, wrinkles, ridges, and minutiae features and that offer cues for gender identification. The goal of this work is to develop novel deep-learning techniques to determine gender from palmprint images. PolyU and CASIA palmprint databases with 90,000 and 5502 images respectively were used for training and testing purposes in this research. After ROI extraction and …

Fracture Resistance Of Simulated External Cervical Resorption In Anterior Teeth Restored With And Without A Fiber Post., Michelle M. Romeo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: External cervical resorption (ECR) is a destructive type of external resorption that can be difficult to treat. ECR usually begins subgingival at the cementoenamel junction of teeth and continues apically and circumferentially. This substantial loss of tooth structure may make these teeth more prone to fracture and should be addressed when treatment planning teeth with ECR. The aim of this in vitro study was to evaluate the fracture resistance of treated and non-treated teeth with simulated ECR using two different treatment modalities.

Materials and Methods: Forty-eight extracted human permanent mandibular central incisors were selected. Teeth were randomly divided into …

Global Existence And Asymptotic Behavior Of The Solutions To Models For Chemotaxis Systems With Chemo Attractants And Repellents, Aesha Lagha

Graduate Theses, Dissertations, and Problem Reports (ETD)

We study global existence and asymptotic behavior of the solutions to models for chemotaxis systems with chemo attractants and repellents in three dimensions. Chemo attractants and repellents may be called chemo agents. For Part I, we use the logistic model for the mass. The interactions between chemo agents and the mass are taken into account. For Part II, we consider the case when mass is conserved and we use the Lotka-Volterra type model for chemo agents. To accomplish this, we use the Fourier transform and energy method. We show the existence of global solutions by the energy method. Also, we …

The Effects Of Membrane Physicochemical Properties On Huntingtin Membrane Association And Downstream Aggregation, Maryssa Beasley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Huntington’s Disease (HD) is a fatal neurodegenerative disorder caused by an expanded glutamine repeat region (polyQ) within the huntingtin protein (htt). As a result of the expanded polyQ domain, htt associates into a variety of toxic aggregate species. The polyQ domain of htt is flanked at the N-terminal end by 17 amino acids (Nt17) that adopt an amphipathic α-helical structure in the presence of binding partners such as lipid membranes. In addition to comprising a lipid binding domain, the Nt17 amphipathic α -helix has been directly implicated in htt aggregation initiation via self-association with other Nt17 α -helices. Due to …

The Interplay Of Progestins, Matrix Metalloproteinases, And The Aging Brain, Keyana Nicole Porter

Graduate Theses, Dissertations, and Problem Reports (ETD)

Progestins are synthetic hormones that are designed to mimic the biological actions of progesterone. They, however, possess other pharmacological actions and properties, in addition to their progestational activities. Medroxyprogesterone Acetate (MPA) is a progestin used globally in the hormonal contraceptive, Depo Provera®, by women in their reproductive prime and is a major compound found in hormone therapy (HT) formulations used by menopausal women. MPA is used by approximately 1 in 5 adolescents and adult women in the United States who are sexually active. Globally, nearly 48 million women utilize injectable contraceptives to prevent pregnancy, with most users utilizing MPA as …

Green Completion As A Mitigation For Btex Exposure In Gas Flaring: Nigeria Case Study (Exposure Assessment And Cost Model), Akintunde S. Akinyemi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gas flaring is an essential part of the oil and gas industry safety and waste management procedures; however, it presents a potential exposure to BTEX compounds, a group of Non-Methane Volatile Organic Compounds (NMVOC), within up to a 10-mile radius of the flare point. The physical and the psychological cost of the journey to work, make employees live close to the workplace. This study examined the perceived exposure of oil and gas industry workers to possible carcinogenic emissions outside the workplace. It looked at the outcomes of the exposure to BTEX through gas flaring drawing on archival data and using …

Applications Of Machine Learning Methods In Health Outcomes Research: Heart Failure In Women, Khalid Abdullah Alhussain

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is robust evidence that heart failure (HF) is associated with substantial mortality, morbidity, poor health-related quality of life, healthcare utilization, and economic burden. Previous research has revealed that there are sex differences in the epidemiology, etiology, and disease burden of HF. However, research on HF among women, especially postmenopausal women, is limited. To fill the knowledge gap, the three related aims of this dissertation were to: (1) identify knowledge gaps in HF research among women, especially postmenopausal women, using unsupervised machine learning methods and big data (i.e., articles published in PubMed); (2) identify emerging predictors (i.e., polypharmacy and some …

Mnemonic Discrimination And Social Anxiety: The Role Of State Anxiety, Gabriella T. Ponzini

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Mnemonic Similarity Task (MST) measures mnemonic discrimination, or the ability to correctly identify new stimuli from highly similar, old stimuli. Neuroscientific and theoretical suppositions suggest that poor mnemonic discrimination may represent a potential risk or maintenance factor for anxious individuals. However, state affect appears to moderate the relation between mnemonic discrimination abilities and trait anxiety. The current study aimed to elucidate the nascent research on mnemonic discrimination and anxiety by evaluating the MST in a specific subtype of anxiety (i.e., social anxiety) and utilizing a clinically relevant stressor task (i.e., knowledge of a future speech). Participants (N = …

Utilizing Conservation Genetics As A Strategy For Recovering The Endangered Candy Darter (Etheostoma Osburni) In West Virginia, Brin E. Kessinger

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Candy Darter (Etheostoma osburni) is a small freshwater fish native to the New River drainage in West Virginia and Virginia that was listed as endangered in November 2018. It has been extirpated from much of its historic range in West Virginia, restricting it to the Gauley and Greenbrier river drainages. In addition to extirpations, the species is threatened by introgressive hybridization with the invasive Variegate Darter (E. variatum). Previous research primarily focused on hybridization, but population genetic analyses were limited. Population genetic analyses aim to identify distinct populations through genetic structure and characterize the levels …

Role Of Ciliary Proteins Adp Ribosylation Factor Like Gtpase 13b (Arl13b) And Bardet-Biedl Syndrome-8 (Bbs8) In Photoreceptor Outer Segment Morphogenesis, Maintenance, And Viability, Tanya L. Dilan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Photoreceptor neurons are modified primary cilia with an extended ciliary compartment known as the outer segment (OS). The mechanisms behind the elaboration of photoreceptor cilia, OS morphogenesis, and maintenance remain poorly understood. In this work, we focused on dissecting the role of two ciliary proteins, the small GTPase ADP-ribosylation factor-like GTPase 13B (ARL13B) and Bardet-Biedl Syndrome-8 (BBS8) in the context of photoreceptor biology. Both BBS8 and ARL13B are linked to defects in ciliogenesis (cilia development) and Retinitis Pigmentosa (vision loss). ARL13B is implicated in regulating ciliary length, and BBS8 is part of the Bardet-Biedl Syndrome complex (BBSome); the BBSome is …