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Graduate Theses, Dissertations, and Problem Reports (ETD)

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Social Anxiety In College Students: The Interaction Of Parental Control And Proximity., Jennifer A. Chrystan Jan 2005

Social Anxiety In College Students: The Interaction Of Parental Control And Proximity., Jennifer A. Chrystan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of the current study was to investigate the relationship between parental control and parental proximity as they influence social anxiety in young adults. Previous research has consistently demonstrated the positive relationship between parental control and social anxiety. However, studies that have examined the desirability of continued parental attachment (proximity) have resulted in a contradictory body of literature. It was hypothesized that two interaction terms, mother protection by mother proximity and father protection by father proximity, would significantly predict participants’ self-reported level of social anxiety after controlling for mother and father parenting variables. Specifically, it was hypothesized that among …

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A Comparison Of Forest Soils Exhibiting Various Degrees Of Nitrogen Availability: Do Tree Species Matter And Which Nutrients Are Limiting In Nitrogen Rich Environments?, Stephen E. Gress Jan 2005

A Comparison Of Forest Soils Exhibiting Various Degrees Of Nitrogen Availability: Do Tree Species Matter And Which Nutrients Are Limiting In Nitrogen Rich Environments?, Stephen E. Gress

Graduate Theses, Dissertations, and Problem Reports (ETD)

Regions downwind of industrial and agricultural pollution receive high amounts of nitrogen (N) deposition. Some forested regions now exhibit symptoms on N saturation, which is N availability in excess of biotic demand. This phenomena and the evidence that trees alter soil in ways that may influence N saturation is the basis of my research. My thesis consists of three parts: (1) Testing the association between tree species and soil properties: Can larger-scale patterns be found under individual trees?; (2) Phosphomonesterase activity in violets (Viola rotundifolia) found in watersheds exhibiting varying levels of nitrogen availability: What lies beyond nitrogen saturation?; and …

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Forestry Transfer Students: A Case Study. West Virginia University Forest Resources Management Curriculum., James B. Harrold Jan 2005

Forestry Transfer Students: A Case Study. West Virginia University Forest Resources Management Curriculum., James B. Harrold

Graduate Theses, Dissertations, and Problem Reports (ETD)

College students are constantly transferring from one university, college or community college to another; or transferring within one university program to another. At West Virginia University, Division of Forestry, Forest Resources Management Curriculum, transfer students are enrolling at an increasing rate each year. This study is an attempt to determine the impact the transfer student has on the Forest Resources Management Curriculum at West Virginia University. The study addressed present and possible future impacts on the curriculum and present problems and suggested solutions associated with the transfer student. Student transfers in the Forest Resources Management Curriculum at West Virginia University …

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Investigation Of The Effects Of Thermal Enrichment And Acid Mine Drainage On Sensitive Aquatic Biota In The Stony River, Grant County, West Virginia., Christopher D. Horn Jan 2005

Investigation Of The Effects Of Thermal Enrichment And Acid Mine Drainage On Sensitive Aquatic Biota In The Stony River, Grant County, West Virginia., Christopher D. Horn

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent surveys have indicated that populations of fishes and crayfish are small in the Stony River, Grant Co., WV. The Stony River is influenced by thermal enrichment and acid mine drainage (AMD) within a 13 km stream reach, downstream of Mt. Storm Lake. In an attempt to determine the influence of each anthropogenic disturbance on these affected taxa, studies of their distribution and survival were undertaken. A crayfish trapping survey was conducted along a gradient of thermal and AMD influences in the Stony River system. Cambarus bartonii was the only species captured, although two subspecies of C. bartonii living in …

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Depositional Environments And Sequence Stratigraphy Of The Rockwell-Price Formation In Western Maryland, South-Central Pennsylvania, And Northern West Virginia, Darin A. Dolezal Dec 2004

Depositional Environments And Sequence Stratigraphy Of The Rockwell-Price Formation In Western Maryland, South-Central Pennsylvania, And Northern West Virginia, Darin A. Dolezal

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Price Formation of northern West Virginia consists of Upper Devonian - Lower Mississippian siliciclastic rocks that are primarily marine. Members of the Price Formation include from oldest to youngest the Oswayo, Cussewago, Riddlesburg Shale, and Rockwell. Rocks of the Price Formation are considered to be of genetic and temporal equivalence to the Rockwell Formation in south-central Pennsylvania, western Maryland, and the eastern West Virginia panhandle.;The research attempts to (1) interpret sedimentary facies and their depositional environments and (2) identify stratigraphic relationships across six outcrops in the central Appalachian basin. Outcrops have been measured and described along a 150 km …

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Effectiveness Of Treatment And Diversity Of Microbial Populations Within A Constructed Wetland Treating Wastewater, Jolene M. Friedland Dec 2004

Effectiveness Of Treatment And Diversity Of Microbial Populations Within A Constructed Wetland Treating Wastewater, Jolene M. Friedland

Graduate Theses, Dissertations, and Problem Reports (ETD)

Constructed wetlands are used as alternative on-site wastewater treatment systems. Microbiological dynamics in constructed wetlands are essential to adequate functioning and efficiency of these systems. The efficacy of wastewater treatment by a large subsurface flow wetland receiving wastewater from a multi-user facility was examined. Real-time monitoring with telemetry was combined with measurement of standard wastewater parameters such as biochemical oxygen demand (BOD5), total suspended solids (TSS), and enumeration of fecal coliforms. Passage of wastewater through the wetland significantly reduced standard wastewater parameters in effluent samples. Heterotrophic bacterial diversity was examined using community level physiological profiling (CLPP) and molecular characterization of …

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Glaucomys Sabrinus Fuscus Habitat And Nest Box Use In West Virginia With Management Recommendations For Kumbrabow State Forest, Tamara M. Terry Dec 2004

Glaucomys Sabrinus Fuscus Habitat And Nest Box Use In West Virginia With Management Recommendations For Kumbrabow State Forest, Tamara M. Terry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nest site selection and habitat use of the endangered West Virginia northern flying squirrel (Glaucomys sabrinus fuscus) is poorly understood. Because access to suitable nesting sites and habitats could be limiting factors, it is important to investigate these ecological requirements to further conservation efforts of the species. My study was conducted on the Kumbrabow State Forest and the MeadWestvaco Wildlife and Ecological Research Forest in Randolph County, West Virginia. I examined characteristics of 31 nest sites (22 cavity; 8 leaf; 1 undetermined) from 4 radio-collared West Virginia northern flying squirrels during the summers of 2002--2003. The nest trees used were …

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Interactions Of Allelopathy And Competition Affecting Ziziphus Spina-Christi And Prosopis Juliflora Seedlings, Thobayet S. Alshahrani Dec 2004

Interactions Of Allelopathy And Competition Affecting Ziziphus Spina-Christi And Prosopis Juliflora Seedlings, Thobayet S. Alshahrani

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study consists of two parts, provides data on the interference between Ziziphus spina-christi, native to Saudi Arabia, and Prosopis juliflora, an introduced species. Dry leaves from both species were milled and used to prepare extracts using 0g/l, 5g/l, 20g/l, 40g/l, 60g/l, and 100 g/l of dried leaves. Leaf extract of P. juliflora was used to irrigate Z. spina-christi with 120 ml weekly over a 7-month growth period, and vice versa. Plant height, plant diameter, number of leaves, leaf area, chlorophyll content, total root length, number of root tips, root diameter, root surface area, root volume, shoot dry weight, root …

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Factors Influencing Basal Area Growth Of Yellow-Poplar ( Liriodendron Tulipifera L.) In Central West Virginia, Christopher T. Crum Dec 2004

Factors Influencing Basal Area Growth Of Yellow-Poplar ( Liriodendron Tulipifera L.) In Central West Virginia, Christopher T. Crum

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper uses data from continuous forest inventory (CFI) plots to evaluate basal area growth of yellow-poplar (Liriodendron tulipifera L.) as related to four topographic factors (aspect, slope position, steepness, and landform), two competition variables (basal area per acre and trees per acre) and four tree characteristics (age, diameter, total height, and crown diameter). These variables were plotted against basal area growth per year using simple linear regression on 69 yellow-poplar trees. The four tree characteristics all had a highly significant (p < .01) relationship with basal area growth. While the other variables did not exhibit a significant relationship, the two competition variables did have a significant (p < .05) relationship with each other.

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Multispectral Segmentation Of Whole-Brain Mri, Nitya Krishnan Dec 2004

Multispectral Segmentation Of Whole-Brain Mri, Nitya Krishnan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Magnetic Resonance Imaging (MRI) is a widely used medical technology for diagnosis and detection of various tissue abnormalities, tumor detection, and in evaluation of either residual or recurrent tumors. This thesis work exploits MRI information acquired on brain tumor structure and physiological properties and uses a novel image segmentation technique to better delineate tissue differences.;MR image segmentation will be important in distinguishing between boundaries of different tissues in the brain. A segmentation software tool was developed that combines the different types of clinical MR images and presents them as a single colored image. This technique is based on the fuzzy …

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Women And Social Services In Saudi Arabia, Khadija Nasseef Dec 2004

Women And Social Services In Saudi Arabia, Khadija Nasseef

Graduate Theses, Dissertations, and Problem Reports (ETD)

This master thesis talks about social services for women that provided by social welfare agencies in Saudi Arabia. This thesis is a descriptive study that uses the content analysis as a method to investigate social services agencies that provides special services for women. This thesis also had special interest in social services that improve women's lives in Saudi Arabia. The content analysis is made on documents and brochures of 4 main social services agencies that offer services for women. The content analysis was made in term of these agencies main aim and target, their main massage, their programs for women, …

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Finite Element Analysis Of Interaction Between Actin Cytoskeleton And Intracellular Fluid In Prechondrocytes And Chondrocytes Subjected To Compressive Loading, Sundar Viswanathan Dec 2004

Finite Element Analysis Of Interaction Between Actin Cytoskeleton And Intracellular Fluid In Prechondrocytes And Chondrocytes Subjected To Compressive Loading, Sundar Viswanathan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Prechondrocytes exhibit a change in their actin cytoskeletal arrangement when subjected to loading. This study looks at the implications of the altered solid-fluid interactions due to this change. A 2-D Finite element model of a cell was developed. A solid-fluid interaction solution procedure was validated and used to determine fluid flow/pressure in a cell when subject to deformation. These values were then used to calculate fluid flow induced stresses on individual actin fibers. The results suggest that prechondrocytes alter their cytoskeletal structure to reduce stresses as a result of fluid flow due to deformation of the cell.

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Comparison Of Chestnut Canker Treatment Procedures For Hypovirus Introduction, Brian C. Bell Dec 2004

Comparison Of Chestnut Canker Treatment Procedures For Hypovirus Introduction, Brian C. Bell

Graduate Theses, Dissertations, and Problem Reports (ETD)

Historically, hypovirulent strains of Cryphonectria parasitica have been introduced into cankers principally by inoculating wounds made to the margin of cankers. Twelve hypovirus introduction methods were tested. Seventy-two trees were inoculated in June 2002 with an orange-pigmented virulent strain of C. parasitica (two inoculations per tree). After 11 weeks, the resulting cankers were treated with a brown-pigmented hypovirulent C. parasitica isolate using one of the following methods: (1) a non-invasive treatment where the inoculum was painted onto the canker surface; (2) an invasive treatment where the canker face was wounded with a sharp blade prior to the painting application of …

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Identity Development And Empathy As Related To Creative Activity Participation, Shana L. Blackshire Dec 2004

Identity Development And Empathy As Related To Creative Activity Participation, Shana L. Blackshire

Graduate Theses, Dissertations, and Problem Reports (ETD)

The focus of this study was the associations between identity, empathy as an emotional construct, and participation in creatively focused activities among adolescents in a largely rural population. A series of measures to assess identity status, the ego strength of fidelity, empathy, and level of participation in creative activities were administered to 212 male and 320 female adolescents in grades nine through eleven at public high schools in Appalachia. Analyses indicated that while the expected relationship between creative activity participation and identity was not statistically significant, gender may heavily influence that relationship as a mediating variable. Empathy, however, was found …

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Potential Mechanisms Linking Nutrition And Neuroendocrine Control Of Reproduction In The Sheep, Adam Bradley Dobbins Dec 2004

Potential Mechanisms Linking Nutrition And Neuroendocrine Control Of Reproduction In The Sheep, Adam Bradley Dobbins

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nutrition and reproduction are linked in that the nutritional status of an individual influences its reproductive performance, i.e. an individual in negative energy balance will have decreased ability to reproduce. The mechanisms in which nutrition influences reproduction are not understood. My data suggest that reductions in lutenizing hormone secretion resulting from photoperiod-induced increases in sensitivity to testosterone negative feedback may be mediated, at least in part, by elevated neuropeptide Y (NPY), a hypothalamic neuropeptide shown to alter gonadotropin releasing hormone release and potently affect food intake. The results from my second study support our hypothesis that the growth hormone mediated …

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Starvation-Induced Alterations In Hepatic Lysine Metabolism In Different Families Of Rainbow Trout (Oncorhynchus Mykiss), Angela Higgins Dec 2004

Starvation-Induced Alterations In Hepatic Lysine Metabolism In Different Families Of Rainbow Trout (Oncorhynchus Mykiss), Angela Higgins

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study focused on lysine catabolism by the lysine alpha-ketoglutarate reductase (LKR) pathway in rainbow trout (RBT). Selective breeding based on feed efficiency in 2 strains (A and B) of RBT generated 2 families within each strain. Eight fish from each of the four families were randomly allotted to individual tanks. Fish were fed until satiation for 3 wks at which time four fish within each family were randomly selected for 2 wks of starvation. After the 2 wk starvation, all fish were harvested. Hepatic in-vitro LKR activity and lysine oxidation (LOX) were measured as was LKR mRNA abundance. Strain …

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Stream Ecosystem Response To Mitigative Limestone Treatment In Acid-Impaired, Central Appalachian Streams, Sarah Elizabeth Mcclurg Dec 2004

Stream Ecosystem Response To Mitigative Limestone Treatment In Acid-Impaired, Central Appalachian Streams, Sarah Elizabeth Mcclurg

Graduate Theses, Dissertations, and Problem Reports (ETD)

We quantified water chemistry, primary production, and benthic macroinvertebrate and fish community structure in 20 Central Appalachian streams: 4 acidic streams, 8 naturally circumneutral streams, and 8 historically acidic streams treated annually with limestone sand over varying lengths of time. The objective was to determine the extent of chemical and biological recovery and temporal trends in the recovery process of limestone treated streams compared to circumneutral reference conditions in Central Appalachia. Results indicate that the application of limestone sand to acidic streams is effective in fully and immediately recovering some of the chemical and biological characteristics of naturally functioning stream …

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Examination Of Experimentally Engineered Larval Fish Habitat In The Marmet Pool, Kanawha River, West Virginia, Jonathan M. Niles Dec 2004

Examination Of Experimentally Engineered Larval Fish Habitat In The Marmet Pool, Kanawha River, West Virginia, Jonathan M. Niles

Graduate Theses, Dissertations, and Problem Reports (ETD)

In rivers, velocity shelters are thought to serve as limiting factors in the retention and recruitment of young fish. Such shelters are formed in backwaters and tributaries, however these areas are limited in the Marmet Pool of the Kanawha River, West Virginia. In response to this concern, several structures that are thought to serve as low velocity zones were placed within the Marmet Pool. This study was conducted to evaluate the experimental rock structures (Finger and Zipper Dikes) as potential mitigation for navigational impact and to determine which sampling gears are best to sample these shallow, structurally diverse areas. The …

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Reasons For Overwinter Declines In Age-1+ Brook Trout Populations (Salvelinus Fontinalis) In Appalachian Headwater Streams, Jeremy J. Webster Dec 2004

Reasons For Overwinter Declines In Age-1+ Brook Trout Populations (Salvelinus Fontinalis) In Appalachian Headwater Streams, Jeremy J. Webster

Graduate Theses, Dissertations, and Problem Reports (ETD)

Brook trout (Salvelinus fontinalis) in the Appalachian Mountains are an important game species in West Virginia, yet angling mortality on these populations in headwater streams is poorly understood. The objectives of this study were to: (1) examine relative changes in body composition of brook trout as they fluctuate throughout the year, and to identify critical periods of survival to determine if brook trout reach critical levels of resource depletion over winter, and (2) evaluate whether angler harvest affects brook trout populations in spring in headwater streams. Body composition estimates indicated that brook trout are unlikely to reach critical levels of …

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Biometric Analysis And Aversive Conditioning Of Black Bears In Southern West Virginia, Harley Wayne Weaver Dec 2004

Biometric Analysis And Aversive Conditioning Of Black Bears In Southern West Virginia, Harley Wayne Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

During 2003, 11 male and 1 female black bear (Ursus americanus) were captured and fitted with radio collars. Individuals were randomly assigned to receive either treatment (n = 6) or control (n = 6). Upon release, treatment individuals received a conditional regimen comprised of rubber buckshot to the flank and a succession of shell crackers. Physical and auditory conditioning techniques we used did not appear to be an effective means of reducing repeat nuisance behavior.;West Virginia Division of Natural Resources (WVDNR) captured 179 (151 males, 28 females) nuisance and 156 (77 males, 79 females) non-nuisance individuals from 1996 to 2003.;We …

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Resonant Optical Waveguide Biosensor Characterization, Shantanu Pathak Dec 2004

Resonant Optical Waveguide Biosensor Characterization, Shantanu Pathak

Graduate Theses, Dissertations, and Problem Reports (ETD)

The importance of miniaturized sensors for quick and accurate assessments of a broad spectrum of hazardous biological agents has been highlighted by recent high profile events. Integrated optical devices based on evanescent wave interrogation techniques can register minute refractive index changes enabling detection of bioagents on the sensor surface and providing a potential for compact structure combined with a possibility of detecting several analytes simultaneously by fabricating multiple devices on the same chip. Most commonly integrated optical interrogation techniques suffer from issues of optical alignment and fabrication complexities. In addition the materials used to fabricate integrated optical devices experience drifts …

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Exposures To Silica And Inducers Of Xenobiotic Metabolism In The Rat Lung, Lori A. Battelli Dec 2004

Exposures To Silica And Inducers Of Xenobiotic Metabolism In The Rat Lung, Lori A. Battelli

Graduate Theses, Dissertations, and Problem Reports (ETD)

CYP1A1 metabolizes polycyclic aromatic hydrocarbons (PAHs), such as those in cigarettes, to reactive intermediates which interact with DNA and lead to cancer. Silica is a lung carcinogen. Epidemiology studies of silica and lung cancer are not all positive. A possible explanation for these inconsistencies may be that silica is a modifier of PAH metabolism and, thus, cigarette smoke carcinogenesis. We hypothesize that crystalline silica exposure alters CYP1A1 expression, thereby modifying lung cancer risk. Rats exposed to both crystalline silica and the model PAH, beta-naphthoflavone (NF), a CYP1A1 inducer have significantly decreased CYP1A1 enzymatic activity and CYP1A1 protein expression. In the …

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Hematotoxicity Of Heptachlor, Sarah Vanessa Meads Dodson Dec 2004

Hematotoxicity Of Heptachlor, Sarah Vanessa Meads Dodson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hematotoxicity has been documented in adults exposed to high levels of organochlorine pesticides. Our studies revealed that prenatal exposure to the organochlorine pesticide heptachlor resulted in alteration of lymphocyte development, while myeloid cell development was spared. To further investigate effects of heptachlor on lymphoid cells, we evaluated direct exposure of this compound on pro-B and pre-B cells. In these studies, the pro-B cell line C1.92 and the pre-B cell line 70Z/3 demonstrated concentration-dependent decreases in proliferation following heptachlor exposure. Pro-B cell viability decreased significantly; however, pre-B cell viability was not altered. Further investigation into mechanisms of diminished cell proliferation revealed …

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Effects Of Herbivory By White -Tailed Deer (Odocoileus Virginianus Zimm.) On The Population Ecology And Conservation Biology Of American Ginseng (Panax Quinquefolius L.), Mary Ann Furedi Dec 2004

Effects Of Herbivory By White -Tailed Deer (Odocoileus Virginianus Zimm.) On The Population Ecology And Conservation Biology Of American Ginseng (Panax Quinquefolius L.), Mary Ann Furedi

Graduate Theses, Dissertations, and Problem Reports (ETD)

American ginseng (Panax quinquefolius L.) is a long-lived understory herb considered rare within its range. Harvesting pressures and habitat degradation/destruction are thought to be the primary causes of population decline although others factors such as browsing by white-tailed deer (Odocoileus virginianus Zimm.) have not yet been explored. To understand the effects of deer browsing on natural ginseng populations, my research focused on four specific objectives. First, I investigated how plant characteristics and microsite conditions influence the susceptibility to being browsed. I found that apparency plays a role in influencing browse selection, such that large, reproductive plants in visible and accessible …

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Augmentation Of The Differentiation Response To Antitumor Quinolines, Rayhana Rahim-Bata Dec 2004

Augmentation Of The Differentiation Response To Antitumor Quinolines, Rayhana Rahim-Bata

Graduate Theses, Dissertations, and Problem Reports (ETD)

An impeding challenge to breast cancer drug therapies is the availability of more effective and less toxic chemotherapeutic agents that do not relay harm to neighboring normal breast cells and tissues. Preliminary studies showed that the quinoline antimalarials, chloroquine (CQ and hydroxychloroquine (HCQ), inhibit proliferation and induce differentiation in breast cancer cell lines without toxicity to normal MCF-10A breast cells. Hence, the goal of these studies was to explore: (1) whether a drug combination modulating epigenetic events would sensitize breast cancer cells to the antitumor activity of CQ or HCQ, (2) and if so, which would be the most promising …

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Intraindividual Variability And Relations Between Daily Affect And Physical Activity Among Community -Dwelling Older Women, Jenessa C. Steele Dec 2004

Intraindividual Variability And Relations Between Daily Affect And Physical Activity Among Community -Dwelling Older Women, Jenessa C. Steele

Graduate Theses, Dissertations, and Problem Reports (ETD)

The current study investigated individual and group-level daily relations between affect and physical activity among 37 non-sedentary community-dwelling older women. The study expected that (a) positive affect would hold a significant, positive synchronous relation with physical activity and (b) negative affect would hold a significant, negative synchronous relation with physical activity over six consecutive days. Women over age 55 (M = 71.5, SD = 9.4) participated by completing four daily assessments (8am, 12pm, 4pm, 8pm) of positive and negative affect and physical activity on a hand-held computer Monday through Saturday. Affect was measured using the Positive and Negative Affect Schedule …

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Modeling And Real-Time Feedback Control Of Mems Device, Limin Wang Dec 2004

Modeling And Real-Time Feedback Control Of Mems Device, Limin Wang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applying closed-loop control to a MEMS devices not only can handle the abnormal behaviors caused by manufactory imprecision or device failure, enabling MEMS devices to survive in critical conditions, but also can increase the application where MEMS devices are used to drive components under varying load conditions. This study mainly focuses on the effort of closed-loop control on the Lateral Comb Resonator (LCR) MEMS device. The success of closed-loop control has been achieved on lateral comb resonator with novel integrated through wafer optical monitoring technique.;Availability of a system model and feedback signals are mandatory conditions for the implementation of closed-loop …

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Visual Basic Time-Space Query Modeling For Geovisualization Of Life Paths, Ryan Heaslip Dec 2004

Visual Basic Time-Space Query Modeling For Geovisualization Of Life Paths, Ryan Heaslip

Graduate Theses, Dissertations, and Problem Reports (ETD)

The origins and incidence of many health problems are related to geographical circumstances. To be able understanding the underlying contributing factors requires conceptualizing the problem in geographical terms. A temporal dataset of individuals' locations was acquired from an annual census taken in Sweden. A space-time query model was constructed to explore associations between an individual's residential locations for given periods of time to those of other individuals with similar heath, or health related factors. The space-time query model was created in the Visual Basic extension of ArcGIS 8.3. Results were in the form of selections that could be exported into …

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Geotechnical Investigation Of Montrose Wetland Site, Christopher R. Ryan Dec 2004

Geotechnical Investigation Of Montrose Wetland Site, Christopher R. Ryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this investigation is to obtain the geotechnical characteristics of the site and to aid in the creation of a proposed engineered wetland at Montrose, West Virginia. The tests performed in the laboratory to obtain the soil properties were: water content, Atterberg limits (liquid and plastic limits), grain size (sieve analysis and hydrometer), specific gravity, field density, and hydraulic conductivity. Tests were performed on soil samples taken from the site during installation of monitoring wells. Results from tests were used in the classification of soil. Additional samples were reconstructed to determine the permeability of the soil. These tests …

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Characterization Of Cyp2d Protein From Human Brain Cerebellum, Deepak Bhatia Dec 2004

Characterization Of Cyp2d Protein From Human Brain Cerebellum, Deepak Bhatia

Graduate Theses, Dissertations, and Problem Reports (ETD)

To date, knowledge in characterization of CYP proteins has been limited to immunoblotting, RT-PCR, immunohistochemistry and so forth; therefore, it is the intention of this study to investigate: (1) What kind of CYP2D protein is present in brain with modern proteomic tools; (2) Whether, CYP2D6 in brain is same as the liver CYP2D6; and (3) If CYP2D7 is present, can CYP2D6 polymorphism be found in the brain?;To answer these questions, this study used a one-step method of isolation of protein by immunoprecipitation followed by its identification using 2D-blots, nanospray LCMS, immunoblots and immunohistochemistry. Probing of immunoprecipitated proteins with polyclonal CYP2D6 …

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