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Mechanism For Heterogeneous Reduction Of Selenite By Zero Valent Iron-Steel Wool., Donglin Huang Jan 2010

Mechanism For Heterogeneous Reduction Of Selenite By Zero Valent Iron-Steel Wool., Donglin Huang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although selenium is as essential element for animal and human growth, selenium released from coal mining process and coal combustion residues to surface and groundwater may cause toxicity to aquatic life. There is urgent need for an easy and accurate analytical method to speciate and determine trace selenium concentrations in waters impacted by coal mine drainage, and treatment technique to remove selenium from aqueous solution. The primary objective of present work is to develop a speciation procedure and analytical method for inorganic selenium species (Se0, SeIV, SeVI) with Multimode Sample Introduction System-Inductive Coupled Plasma Optic Emission Spectrometer (MSIS-ICP). Experiments showed …

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Nonwoven Geotextile Filtration Performance With Coal Refuse, Rajesh Tolikonda Jan 2010

Nonwoven Geotextile Filtration Performance With Coal Refuse, Rajesh Tolikonda

Graduate Theses, Dissertations, and Problem Reports (ETD)

The project involved bench scale testing investigating geotextile filter clogging by fine particle intrusion and fabric blinding from coarse and fine coal refuse with nonwoven geotextile filter fabric. The research explored the fine particle distribution through the refuse material and interfacial contact with the geotextile. The coal refuse was obtained from an active mining and coal waste impoundment operation located in Boone County, WV. The research involved testing of non-woven geotextiles with coarse and fine coal refuse under standard and reduced compaction energies in order to simulate field performance of the fabric to the effects of loose compaction conditions, such …

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Numerical Modeling And Field Monitoring Of Overburden Response During Geologic Sequestration, Raj Kumar Gondle Jan 2010

Numerical Modeling And Field Monitoring Of Overburden Response During Geologic Sequestration, Raj Kumar Gondle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Atmospheric levels of carbon dioxide (CO2) have increased at a rate of about 1 to 2 parts per million (ppm) per year to its current level of approximately 388 ppm. Mitigation efforts are being deployed around the world in all possible ways to combat these accelerating levels of carbon dioxide. The study presented in this dissertation deals with the ground response caused by geologic sequestration of carbon dioxide. The ground response can be used as a tool for long-term monitoring of carbon storage in geological formations.;Unmineable coal seams have been identified as promising reservoirs for large-scale sequestration of carbon dioxide. …

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Selenium Removal By Nano-Magnetite Impregnated Diatomaceous Earth, Isabel Cardona Jan 2010

Selenium Removal By Nano-Magnetite Impregnated Diatomaceous Earth, Isabel Cardona

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to remove selenium from aqueous solution using nano-magnetite impregnated diatomaceous earth (DE) as an adsorbent. The adsorbent was prepared at the laboratory with a ratio of magnetite/DE of 10%. Batch adsorption experiments were conducted to evaluate the effects of pH, temperature, adsorbent concentration, selenium speciation, contact time and presence of competing anions on selenium removal efficiency in conjunction with studies on adsorption kinetics, isotherms, and adsorptive thermodynamics. Rapid adsorption occurred within 30 min and selenium uptake decreased with increase in temperature. Low pH values (i.e. 2--4) favored selenium adsorption as expected for anion adsorption …

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Reservoir Modeling Of New Albany Shale, Amirmasoud Kalantari-Dahaghi Jan 2010

Reservoir Modeling Of New Albany Shale, Amirmasoud Kalantari-Dahaghi

Graduate Theses, Dissertations, and Problem Reports (ETD)

The intent of this study is to reassess the potential of New Albany Shale formation using a novel and integrated workflow, which incorporates field production data and well logs using a series of traditional reservoir engineering analyses complemented by artificial intelligence & data mining techniques. The model developed using this technology is a full filed model and its objective is to predict future reservoir/well performance in order to recommend field development strategies.;The impact of different reservoir characteristics such as matrix porosity, matrix permeability, initial reservoir pressure and pay thickness as well as the length and the orientation of horizontal wells …

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The Impact Of Formation Characteristics On Co2 Sequestration Into An Aquifer, Nagi A. Lam Jan 2010

The Impact Of Formation Characteristics On Co2 Sequestration Into An Aquifer, Nagi A. Lam

Graduate Theses, Dissertations, and Problem Reports (ETD)

With the increasing trends in global temperature and CO2 emissions, there is expanding interest in geological storing as potential sites for the safe and fast disposal of CO2. This expanding interest necessitates the need of studying the effects of geological parameters on CO2 sequestration. The processes that occur in CO2 sequestration are complex with many different parameters influencing the behavior of the injected CO2 and therefore numerical simulations are required.;The objective of this research is to study the impact of the aquifer properties and operational parameters to understand the CO2 plume behavior and their contribution to various trapping mechanisms. Such …

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State Spatial Practices And Uneven Development: Flexible Rescaling In Appalachia, Jacquelyn Jordon Core Jan 2010

State Spatial Practices And Uneven Development: Flexible Rescaling In Appalachia, Jacquelyn Jordon Core

Graduate Theses, Dissertations, and Problem Reports (ETD)

Geographic theories regarding uneven development, including Neil Smith's convergence of Marxist theory and spatial development and David Harvey's development of scalar fix and later conceptual development of the process of capital accumulation, historically have focused primarily on how and why uneven development occurs. Research to date has provided only a cursory examination of what role, if any, the state plays in impacting uneven development, has not endeavored to characterize the state as an active, purposeful spatial player in the process of uneven development, and has not conceptualized how the state impacts uneven development through spatial practices, if the state does …

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The Europeanization Of French, German And British China Policies: The Case Of The Arms Embargo On China, Vivien L. Exartier Jan 2010

The Europeanization Of French, German And British China Policies: The Case Of The Arms Embargo On China, Vivien L. Exartier

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study analyzes the impact of France, Britain and Germany on European Union sanction policy on China. The study argues that Britain, France and Germany affect the EU sanction policy on China through bottom-up and horizontal Europeanization due to their historic and strategic ties to the PRC and their predominance in the armament industry. The study specifically reviews the case of the handling of the lift of the arms embargo between 2003 and 2005, imposed on the wake of the Tiananmen Square Massacre in 1989. The methodology combines a questionnaire, addressed to defense and foreign affairs officials from the three …

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Numerical Modeling Of Pressure Transient Behavior Of Fractured Coal Samples, Rupesh Kumar Gondle Jan 2010

Numerical Modeling Of Pressure Transient Behavior Of Fractured Coal Samples, Rupesh Kumar Gondle

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the current study the pressure transient behavior of various coal samples was investigated by using the finite element approach. Finite element analyses were performed on fourteen coal samples with different physical dimensions. Two pressure transients, namely Sine-6 and A-Spike pressure pulses, were used in the research study. Fluid compressibility values of CO2 and Argon were used to perform the analyses. A fracture width of 1mm was considered for each sample to investigate the influence of fluid type on coal permeability. The influence of elastic modulus of coal sample, fracture porosity, fracture width, and fluid compressibility were investigated. The finite …

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Extracellular Matrix Mediated Cartilage Regeneration: A Novel Three-Dimensional In Vitro Microenvironment To Facilitate Cell Expansion And Chondrogenesis, Fan He Jan 2010

Extracellular Matrix Mediated Cartilage Regeneration: A Novel Three-Dimensional In Vitro Microenvironment To Facilitate Cell Expansion And Chondrogenesis, Fan He

Graduate Theses, Dissertations, and Problem Reports (ETD)

Joint articular cartilage is vulnerable for degenerative disease after trauma due to the limited self-healing ability. Mesenchymal stem cells (MSCs) are considered a promising source for cartilage repair and regenerative medicine because of their excellent capacities of self-renewal and multipotent differentiation. Conventional in vitro expansion system, however, limits the utilization of MSCs for cartilage regeneration because in monolayer culture MSCs rapidly lose their self-renewal ability and differentiation potential. The lack of proper microenvironment in the current two-dimensional expansion system is attributed to the loss of self-renewal and multipotent differentiation in MSCs. Three-dimensional (3D) cell-deposited extracellular matrix (ECM) provides a novel …

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Listeria Survival After Isoelectric Solubilization And Precipitation Of Fish Protein With Organic Acids, Rachel Otto Jan 2010

Listeria Survival After Isoelectric Solubilization And Precipitation Of Fish Protein With Organic Acids, Rachel Otto

Graduate Theses, Dissertations, and Problem Reports (ETD)

With mechanical fish processing, a substantial amount of protein is discarded as byproducts. Isoelectric solubilization and precipitation (ISP) is a process that recovers previously discarded protein using extreme pH shifts to solubilize and precipitate protein from byproducts. Typically, strong acids are used for pH reduction but have limited impact on bacterial load; therefore, organic acids were used during ISP processing to test the impact on Listeria ssp. concentrations. The goals of these studies were to determine the effectiveness of organic acids, specifically acetic and citric acids, to reduce L. innocua during the protein recovery process of ISP and to determine …

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Applications Of Krische Allylation Towards Natural Product Synthesis., Melvin Rajaratnam Jan 2010

Applications Of Krische Allylation Towards Natural Product Synthesis., Melvin Rajaratnam

Graduate Theses, Dissertations, and Problem Reports (ETD)

A short asymmetric synthesis of the anti-cancer natural product Rugulactone has been attempted. The synthetic route begins with a mono-Krische allylation of 1,3-propane diol resulting in (S)-hex-5-ene-1,3-diol. Acylation followed by ring closing metathesis (RCM) led to the desired α-pyranone core. Similarly, we focused on the bis-Krische allylation, selective acylation and the RCM sequence towards total synthesis of various αpyranone natural products and their analogues. The progress toward this synthesis is described.

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Seeing Science Everywhere: A Case Study Of The Perceptions Of Three Fifth Grade Science Teachers' Complementary Use Of The Arts In The Science Classroom In An Economically Distressed County In Central Appalachia., Angela A. Mckeen Jan 2010

Seeing Science Everywhere: A Case Study Of The Perceptions Of Three Fifth Grade Science Teachers' Complementary Use Of The Arts In The Science Classroom In An Economically Distressed County In Central Appalachia., Angela A. Mckeen

Graduate Theses, Dissertations, and Problem Reports (ETD)

By employing qualitative methods, this study sought to determine the perceptions three fifth grade science teachers held about using the arts to complement science teaching in an economically distressed county in rural Appalachia and the perceived roles the arts and sciences play in the lives of those living in rural Appalachia. Each case was studied using participant-observer observations, personal journals, archival data, and interviewed using semi-structured, in-depth interview techniques. From these data, unique themes emerged for each of the three cases. Constant comparative analysis was used to analyze the data set for each individual case. A cross-case analysis was then …

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Memorializing Motherhood: Anna Jarvis And The Struggle For Control Of Mother's Day., Katharine Lane Antolini Jan 2010

Memorializing Motherhood: Anna Jarvis And The Struggle For Control Of Mother's Day., Katharine Lane Antolini

Graduate Theses, Dissertations, and Problem Reports (ETD)

Few people outside the state of West Virginia recognize the name Anna Jarvis or know of the lovely church that stands as a shrine to her accomplishments. Yet, on every second Sunday in May, we mail the cards, buy the flowers, place the long distant phone calls, or make the brunch reservation to honor our mothers because of her. Anna Jarvis was the founder of Mother's Day. She organized the first official Mother's Day celebration in 1908 and then spent four decades promoting the holiday and defending it from commercialization and ideological exploitation. Jarvis designed her Mother's Day celebration based …

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The Effects Of Fungal Pigments On Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectral Peak Formation, Amanda Dawn Buskirk Jan 2010

The Effects Of Fungal Pigments On Matrix-Assisted Laser Desorption/Ionization Time-Of-Flight Mass Spectral Peak Formation, Amanda Dawn Buskirk

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fungi are ubiquitous microorganisms that commonly cause diseases with high mortality rates in immunocompromised hosts. In order to successfully treat the infection and subsequently decrease mortality rates, it is necessary to rapidly identify the causal organism(s) and promptly begin the proper treatment. Common identification methods, such as viable culture characterization, are often time consuming or not species-specific, as is the case with many molecular or immunodiagnostic assays. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been used to discriminate between many clinically relevant microorganisms. Recently, MALDI-TOF MS has been used to discriminate between monilaceous fungal species, however, darkly pigmented …

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The Effects Of Non-Thermal Electron Beam Irradiation On Quality Indicators Of Peanut Butter, Amanda El-Rawas Jan 2010

The Effects Of Non-Thermal Electron Beam Irradiation On Quality Indicators Of Peanut Butter, Amanda El-Rawas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electron beam (e-beam) irradiation is an effective non-thermal processing step for the reduction of Salmonella in peanut butter. The objective of this study was to evaluate the effects of e-beam irradiation on the quality of peanut butter. Peanut butter samples were exposed to e-beam doses of 0 (control), 3.2, 7.0, or 27.7 kGy, confirmed by film dosimetry. Doses correlated with a 5- and 12-log reduction of Salmonella respectively; with 27.7 kGy representing an extreme dose. Samples were examined over a 15-day period at 22°C. Color analysis (L, a*, b*), spreadability, and thiobarbituric acid reactive substances test (TBARS) for lipid oxidation …

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Global Mindedness And Dispositions Towards Diversity In The Classroom, Ras T. Acolatse Jan 2010

Global Mindedness And Dispositions Towards Diversity In The Classroom, Ras T. Acolatse

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examined global mindedness and dispositions towards diversity in the classroom between two groups of teacher candidates. One group had obtained a bachelors degree before starting the teacher education program while the other group started the teacher education program without a college degree. The global mindedness scale (GMS) and the Teacher Multicultural Attitude Scale developed by Hett (1993) and Ponterrotto (2004) respectively were used for the study.;The study was guided by four research questions and four corresponding hypotheses. Univariate t-tests were use to analyze data for research questions one and two to determine the difference between the mean scores …

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Comprehensive Tobacco Control Policy Regimes And Population Health: Assessing Causal Loops, Stephanie J. Frisbee Jan 2010

Comprehensive Tobacco Control Policy Regimes And Population Health: Assessing Causal Loops, Stephanie J. Frisbee

Graduate Theses, Dissertations, and Problem Reports (ETD)

Tobacco products remain among the most controversial consumer products of all time: cigarettes are the only legal product that, when used as intended, are lethal. The global and individual burden attributable to the primary use of tobacco, or secondary or tertiary exposure to cigarette smoke, whether measured by morbidity, mortality, or economic costs, is substantial. With the combined efforts of scientific research, public health and policy advocates, the image and use of tobacco products has undergone profound change. However, while much has been achieved regarding the attitudinal, behavioral, and policy changes needed to diminish the individual, social, and economic costs …

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Mechanical Degradation Of Thermal Properties Of Flexible Aerogel Blankets, Brice G. Gnahore Jan 2010

Mechanical Degradation Of Thermal Properties Of Flexible Aerogel Blankets, Brice G. Gnahore

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aerogel materials are of significant interest to numerous applications in many areas of engineering such as oil, gas, aerospace, military, construction, building and apparel because they offer insulating properties that outperform traditional materials by a factor ranging from 2 to 8. Although an aerogel can be manufactured in a clamshell, sleeve or sheet shapes, the most versatile form is a blanket. Aerogel blankets are composites of silica aerogel particles dispersed in a reinforcing fiber matrix that turns the brittle aerogel into a durable and flexible insulating mat.;While aerogel blankets exhibit excellent thermal properties, they are still some concerns over dust …

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A Case Study Of Universal Design For Learning Applied In The College Classroom, Marie E. Leichliter Jan 2010

A Case Study Of Universal Design For Learning Applied In The College Classroom, Marie E. Leichliter

Graduate Theses, Dissertations, and Problem Reports (ETD)

As the landscape of education and the demographics of the postsecondary classroom continue to evolve, so too must the teaching practices at our nation's institutions of higher education. This study follows an instructor who has evolved to incorporate Universal Design for Learning (UDL) techniques into her classroom, even though prior to participation in this research study, she had not heard of UDL. UDL is a flexible framework used to design curricula that enable all learners to acquire knowledge, skills, and motivation to learn. This qualitative, descriptive case study addressed how and to what extent UDL techniques are being implemented in …

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Phospholipid Enhanced Separation With Capillary Electrophoresis, Ruijuan Luo Jan 2010

Phospholipid Enhanced Separation With Capillary Electrophoresis, Ruijuan Luo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Phospholipids were used as an additive in capillary electrophoresis to enhance the separation of glycans derived from alpha1-acid glycoprotein, fetuin, and ribonuclease B. The properties of phospholipid preparations were dependent upon composition, hydration, and temperature. Separation performance was evaluated as a function of these variables. A preparation of 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1,2-dihexanoyl-snglycero-3-phosphocholine (DHPC), with [DMPC]/[DHPC] = 2.5, in 10% lipid/aqueous buffer at 25 °C provided the best separation efficiency at an electric field strength of 400 V/cm. Resolution was enhanced with the additive as well.;Phospholipids were also investigated as an additive for capillary electrophoresis separation of DNA molecules. Lipid preparations …

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Genetic Characterization, Ginsenoside Analysis And Micropropagation Of American Ginseng (Panax Quinquefolius L.), Samuel G. Obae Jan 2010

Genetic Characterization, Ginsenoside Analysis And Micropropagation Of American Ginseng (Panax Quinquefolius L.), Samuel G. Obae

Graduate Theses, Dissertations, and Problem Reports (ETD)

American ginseng (Panax quinquefolius L.), is an economically important medicinal plant native to eastern deciduous forests of North America. Like many other medicinal plants worldwide, P. quinquefolius is mainly collected from the wild. And because of its growing demand, abundance of wild populations is threatened with over harvesting. In West Virginia, P. quinquefolius is available both in cultivation and in the wild. Even though P. quinquefolius occurs in all 55 counties in West Virginia, patterns of its harvesting and cultivation vary across the state. As described in chapter 2, the genetic diversity and population structure of P. quinquefolius under two …

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Sensitivity Of Semantic Signatures In Text Mining, Sri Ramya Peddada Jan 2010

Sensitivity Of Semantic Signatures In Text Mining, Sri Ramya Peddada

Graduate Theses, Dissertations, and Problem Reports (ETD)

The rapid development of the Internet and the ability to store data relatively inexpensively has contributed to an information explosion that did not exist a few years ago. Just a few keystrokes on search engines on any given subject will provide more web pages than any time before. As the amount of data available to us is so overwhelming, the ability to extract relevant information from it remains a challenge.;Since 80% of the available data stored world wide is text, we need advanced techniques to process this textual data and extract useful in formation. Text mining is one such process …

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Framing And Fundraising: Emotional Language And Money Raised In A Vietnamese Newspaper Column, Hong Van Pham Thi Jan 2010

Framing And Fundraising: Emotional Language And Money Raised In A Vietnamese Newspaper Column, Hong Van Pham Thi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This exploratory study examined Vuot Len So Phan , a Vietnamese philanthropy newspaper column in Sai Gon Tiep Thi, a Vietnamese newspaper from April to December 2009. Guided by framing literatures, 39 episodic frames stories portraying financially disadvantaged people were content analyzed in terms of demographics of the featured subjects and emotional phrases used in the stories. The hypothesis predicting a positive correlation between emotional phrases and money raised was supported. This means the more emotional phrases used in an episodically framed story, the more money it was likely to raise. This study found an unexpected result that donations were …

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Chemotherapy-Induced Damage Of The Bone Marrow Microenvironment, Stephanie Lynne Rellick Jan 2010

Chemotherapy-Induced Damage Of The Bone Marrow Microenvironment, Stephanie Lynne Rellick

Graduate Theses, Dissertations, and Problem Reports (ETD)

The bone marrow microenvironment is characterized as the anatomical site including specialized niches that support stem cells. In addition, these niches also provide both soluble and physical cues leading to the differentiation of stem cells into all the cells of the blood. The studies in this dissertation focus on two supportive niches in the bone marrow microenvironment, osteoblasts (HOB) and bone marrow stromal cells (BMSC), in the setting of high dose chemotherapy and the potential damage that chemotherapy treatment causes to the cells of the bone marrow microenvironment.;In the first study, we investigated the effects of melphalan and Etoposide (VP-16) …

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Development Of Nanofluidic/Microfluidic Interfaces As Analyte Concentrators For Proteomic Samples, Kathleen C. Reschke Jan 2010

Development Of Nanofluidic/Microfluidic Interfaces As Analyte Concentrators For Proteomic Samples, Kathleen C. Reschke

Graduate Theses, Dissertations, and Problem Reports (ETD)

We have developed a nanofluidic microfluidic analyte concentrator for application to proteomic samples. This dissertation includes a study investigating the current rectification behavior of the device including a temporal characterization of the ON state to OFF state transition and the OFF state to ON state transition as a function of device design. An analyte concentrator compatible with proteomic samples is presented and the current rectifying phenomenon is utilized to ensure adequate analyte delivery for the enrichment process. Finally, characterization of a coating technique to alter the surface charge of the nanofluidic element from anionic to cationic is presented.

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Intergenerational Transmission Of Health-Promoting Behaviors: Examining Participation In Physical Activity Between Middle-Aged Mothers And Their Younger Adult Children, Sarah T. Stahl Jan 2010

Intergenerational Transmission Of Health-Promoting Behaviors: Examining Participation In Physical Activity Between Middle-Aged Mothers And Their Younger Adult Children, Sarah T. Stahl

Graduate Theses, Dissertations, and Problem Reports (ETD)

Research has provided considerable evidence that participation in regular physical activity is associated with numerous physical and mental health benefits (Penedo & Dahn, 2005). Despite public health efforts to increase the activity levels adults, only 25% of the U.S. adult population is regularly active and nearly 60% remains sedentary (US Department of Health and Human Services [USDHHS], 2008). A small, but growing, area of research has examined physical activity from an intergenerational or dyadic perspective that considers how involvements in close, personal relationships influence levels of physical activity. In a sample of middle-aged mothers and their younger adult children, the …

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Preliminary Analysis Of The Environmental Effects On Rna Degradation: Modeling A Realistic Crime Scene, Beatriz A. Vianna Jan 2010

Preliminary Analysis Of The Environmental Effects On Rna Degradation: Modeling A Realistic Crime Scene, Beatriz A. Vianna

Graduate Theses, Dissertations, and Problem Reports (ETD)

In forensic science, polymerase chain reaction (PCR) became an indispensable tool given the limited amount of biological samples usually encountered at crime scenes. DNA analysis is used to identify the source of biological samples typically obtained from a single hair, or droplet of blood. Determining the source of the biological evidence can provide a spatial link, thereby including or excluding a suspect at a crime scene or other locations related to a crime investigation. In spite of the great efficiency in human identification, DNA analysis cannot provide any information regarding time of deposition of the sample. The ability to establish …

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Dynamic Bandgap Tuning Of Solid Thin Film Photonic Crystal Structures, Hyma Yalamanchili Jan 2010

Dynamic Bandgap Tuning Of Solid Thin Film Photonic Crystal Structures, Hyma Yalamanchili

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanophotonics, specifically photonic crystals (PhCs), offer unique optical bandgap engineering possibilities that has driven the emergence of a variety of device platforms, including: beam splitters, nano-cavity resonators, lasers, fibers, waveguides and highly sensitive optofluidic biosensor devices. The design and fabrication of accurate lattice parameters for a PhC is very important to achieving the desired operating bandgap. The inclusion of tunability in thin film PhCs not only offers a means of adjusting for fabrication errors but also a mechanism to increase device functionality as well as providing a wider range of operating wavelengths. Nitride thin films, specifically Aluminum Nitride (AlN) and …

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The Role Of Nonmuscle Myosin Iia In Endothelial Cell, Jing Zhu Jan 2010

The Role Of Nonmuscle Myosin Iia In Endothelial Cell, Jing Zhu

Graduate Theses, Dissertations, and Problem Reports (ETD)

Myosins are molecular motors that convert chemical energy into mechanical force. Vertebrate cells contain three myosin II isoforms, designated as myosin IIA, IIB and IIC, and each isoform has unique biochemical and biophysical charasterics. It is generally accepted that all three isoforms are involved in regulating actin structures and cell contraction. However, it is unknown what role(s) each isoform plays in maintaining actin structures and generating cellular force. Experiments presented in this thesis will begin to document what role myosin IIA plays in endothelial cell contraction and actin dynamics.;Myosin IIA ablated from endothelial cells by infection with adenovirus encoding a …

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