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Targeting Vulnerabilities In Cell State And Calcium Signaling For The Treatment Of Lung Cancer, Clark Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer remains the deadliest of all cancers due to the high mutational burden associated with the disease. Combating mutational drivers and drug resistance proves to be essential in the development of novel therapies to improve patient outcomes. A first-in-class cyclic peptide known as MTI-101 has been shown to induce necrotic cell death in a caspase independent manner. MTI-101 was derived from the linear peptide known as HYD1 that was found in a high throughput screen to block cell adhesion with the extracellular matrix. The compound was further optimized and cyclized to the currently used MTI-101 that was found to …

Analysis Of Forensically Relevant Evidence Using Electrochemistry, Spectroscopy, And Mass Spectrometry Tools, Colby Edward Ott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forensic science relies on the use of multiple techniques in the assessment of evidence to increase the accuracy and reliability of the results. However, with the rapidly changing drug landscape due to the introduction of novel psychoactive substances, many traditional screening methods are no longer sensitive or selective enough for use. Additionally, many screening methods such as chemical color tests are prone to false positive and negative results and are subjective. Therefore, the goal of this dissertation was to develop a novel analytical scheme that can provide a more efficient, rapid, and sensitive method that will facilitate adoption in the …

Reinforcement Learning For Process Control: Applications To Energy Systems, Elijah Ballard Hedrick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Reinforcement learning (RL) is a machine learning method that has recently seen significant research activity owing to its successes in the areas of robotics and gameplaying (Silver et al., 2017). However, significant challenges exist in the extension of these control methods to process control problems, where state and input signals are nearly always continuous and more stringent performance guarantees are required. The goal of this work is to explore ways that modern RL algorithms can be adapted to handle process control problems; avenues for this work include using RL with existing controllers such as model predictive control (MPC) and adapting …

Investigating The Mitochondrial Protein Mitoneet In C. Elegans Models Of Aging And Alzheimer's Disease, Jacob Ryan Boos

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oxidative stress is an imbalance between reactive oxygen species production and elimination, favoring the former. Reactive oxygen species serve as important signaling molecules for physiological homeostasis. However, when produced in excess, these once important signaling molecules become detrimental, disrupting cellular functions, and ultimately leading to cell death. In aging, reactive oxygen species production is increased, accompanied with reductions in oxidative stress resistance, increasing the risk for developing age-related diseases including cardiovascular disease, cancer, stroke, and neurodegenerative diseases. The outer mitochondrial membrane iron-sulfur cluster containing protein mitoNEET (CISD1; gene CISD1) has shown to be a mediator of mitochondrial function and …

Machine Learning Approach To Health Risk Determination, Levi Butcher Cole

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, cardiovascular disease is the leading cause of death for both men and women. The factors that contribute to heart disease are well known but it is still an ever-evolving field of research. The possible benefits to being able to determine an individual’s Health Risk could provide the early insights needed to combat cardiovascular disease. However, two major problems within this area are the public availability of a dataset and the label imbalances for the dataset. In this thesis, we present a framework for exploring health risk using a combination of the NHANES dataset and open-source machine …

Vaping During Pregnancy: Effects On Vascular And Behavioral Outcomes In Offspring, Eiman A. Aboaziza

Graduate Theses, Dissertations, and Problem Reports (ETD)

Electronic cigarette (e-cig) use is increasing due to aggressive marketing, tempting flavors, and seemingly higher acceptability in the community (lesser perceived social stigma) despite unproven claims of safety. In an alarming trend, pregnant women smokers have turned to novel “modified risk” products, such as e-cigs, in response to heavy marketing of e-cigs as safer alternatives to cigarettes and a tool to help quit smoking. This is despite proven detrimental effects of nicotine on a growing fetus, and scarcity of information regarding toxicity of e-liquid (with and without nicotine) on child development. Moreover, rampant e-cig use among youth (nearly 4 million …

Evaluate The Efficacy Of A Mixture Of Peroxyacetic Acid And H2o2 Against The Survival And Cross-Contamination Of The Salmonella Surrogate Enterococcus Faecium On Tomatoes During Triple-Wash, Corey Waitman Coe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Triple-wash with a mixture of peroxyacetic acid and H2O2 (SaniDate-5.0) during post-harvest processing of fresh produce has been recommended by West Virginia Small Farm Center to improve microbial safety. It has been well recognized that the washing of produce is more important for preventing cross-contamination than reducing foodborne pathogens. Furthermore, it may help improve public confidence in that the produce they obtain from locally grown farmers is safe for their consumption. determine the efficacy of SaniDate-5.0 for reducing the survival and preventing cross-contamination of the Salmonella surrogate Enterococcus faecium on tomatoes during triple-wash.

E. faecium ATCC-8459 (resistant to 100-ppm nalidixic-acid) …

High Precision Pipeline Leak Detection And Localization Using Negative Pressure Wave Technique: An Application In A Real Field Case Study, Nathaniel C. Moryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

One of the most important aspects of oil and gas production is the safe and efficient fluid transportation using pipelines. Pipelines transporting various fluids are the most efficient but are susceptible to failure and leaks. These leaks can come about through natural disaster, as well as from general wear from the pipes that could result in major environmental and economic problems. The ability to detect leaks with speed and accuracy, as well as locating these leaks within a narrow range, will aid with the maintenance response. Hasty responses will minimize the revenue loss and reduce potential environmental impact but bring …

Exploiting Modulation Of The Blood-Brain And Blood-Tumor Barrier Permeability By Translational Focused Ultrasound For Therapeutic Delivery To Cns Metastases, Tasneem A. Arsiwala

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transcranial low-intensity focused ultrasound is a unique technology to modulate the integrity of tight endothelial junctions and transiently increase BBB/BTB permeability to enhance therapeutic delivery. Despite promising early studies, present literature lacks agreement on key experimental conditions, which restricts our knowledge and the technique's widespread translation. This dissertation first provides a critical review of the current gaps in knowledge regarding the universal use of LiFUS in preclinical and clinical use. We then identify key parameters for translational and predictable opening of the BBB using a 3T MRI coupled with a clinical device. Our investigation highlights that passive permeability of the …

Precise Landing Of Vtol Uavs Using A Tether, Jeremy W. Rathjen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Unmanned Aerial Vehicles (UAVs), also known as drones, are often considered the solution to complex robotics problems. The significant freedom to explore an environment is a major reason why UAVs are a popular choice for automated solutions. UAVs, however, have a very limited flight time due to the low capacity and weight ratio of current batteries. One way to extend the vehicles' flight time is to use a tether to provide power from external batteries, generators on the ground, or another vehicle. Attaching a tether to a vehicle may constrain its navigation but it may also create some opportunities for …

System Development Of An Unmanned Ground Vehicle And Implementation Of An Autonomous Navigation Module In A Mine Environment, Jonas Amoama Bredu Jnr

Graduate Theses, Dissertations, and Problem Reports (ETD)

There are numerous benefits to the insights gained from the exploration and exploitation of underground mines. There are also great risks and challenges involved, such as accidents that have claimed many lives. To avoid these accidents, inspections of the large mines were carried out by the miners, which is not always economically feasible and puts the safety of the inspectors at risk. Despite the progress in the development of robotic systems, autonomous navigation, localization and mapping algorithms, these environments remain particularly demanding for these systems. The successful implementation of the autonomous unmanned system will allow mine workers to autonomously determine …

Heterogeneous Oxidation Of Multi-Component Aqueous Organic Aerosols: The Effect Of Transport Phenomena And Reaction Compartment On Reaction Kinetics., Tadini Wenyika Masaya

Graduate Theses, Dissertations, and Problem Reports (ETD)

The surface-bulk partitioning of small organic molecules in aqueous droplets was investigated using molecular dynamics. The air-particle interface was modeled using a 80-Å cubic water box containing series of organic molecules and surrounded by gaseous OH radicals. The properties of the organic solutes within the interface and the water-bulk were examined at a molecular-level using density profiles and radial pair distribution functions. Molecules containing only polar functional groups such as urea and glucose are found predominantly in the water bulk, forming an exclusion layer near the water surface. Substitution of a single polar group by an alkyl group in sugars …

Living On The Edge: Trans* Exclusion, Survival, And Resistance, Rayna E. Momen

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

This dissertation focuses on the criminalization and victimization of transgender people, broadly defined as people whose gender identity does not align with their assigned birth sex, using a queer criminological and intersectional lens. The introduction offers background and context for this examination, and includes the purpose, theoretical approach, methodology, research questions, contributions, and overviews of the substantive chapters. Chapter two explores what we know and what we do not know about transgender people’s pathways into the criminal legal system, how they experience state surveillance, and how they navigate the system. Chapter three explores violence against transgender people in various …

Noise Exposure Trends Among Violating Coal Mines, 2000 To 2021, Hanna Grace Davis

Graduate Theses, Dissertations, and Problem Reports (ETD)

Noise-induced hearing loss has continued to be a significant issue within the mining industry, even after the passing of the MSHA’s improved noise standard in 2000. NIOSH has recognized that the noise standard enacted by both MSHA and OSHA does not properly protect workers with the 5 dBA exchange rate (i.e., the increase in noise level leading to a doubling of energy) and the lack of emphasizing the use of engineering controls over administrative controls and overreliance on hearing protection device usage. The primary objective of this study is to investigate statistical sound relationships between MSHA noise-related violations and samples …

Exploration Of Intercollegiate Student-Athletes’ Athletic Identity, Leah C. Oldham

Graduate Theses, Dissertations, and Problem Reports (ETD)

Student-athletes encounter experiences that can cause their identity process and self-concept to be disturbed. Even though athletic identities are strong among intercollegiate athletes, some encounter identity-threatening events that interrupt their identity process, negatively affecting their athletic identity. The present study adds to current research on the athletic identity of student-athletes by examining the athletic identity and self-esteem of student-athletes to determine if a redshirt season is a positive (identity-enhancing) or negative (identity-threatening) event. The intersectionality of age, race, gender, and ethnicity are examined to understand if multiple identities influence the experience of a redshirt season. Results reveal that a redshirt …

Characterizing Locomotor Disturbance Perception In Young Adults, Daniel James Liss

Graduate Theses, Dissertations, and Problem Reports (ETD)

Falls during walking are a leading cause of injuries across aging. Many of these falls are due to slips and trips. The ability to perceive disturbances to ongoing motion may play an important role in the control of walking balance. However, disturbance perception has been investigated in standing balance, but its role in walking balance due to slip- and trip-like disturbances remains largely unknown. Characterizing locomotor disturbance perception in young adults may lead to a more comprehensive understanding of sensorimotor walking balance control.

This work defined locomotor disturbance perception in response to slip and trip-like disturbances in young adults. We …

Evaluating Potential Soil Amendments And Geomorphic Methods For Mine Land Reclamation, Amanda Rodrigues Silva

Graduate Theses, Dissertations, and Problem Reports (ETD)

The United States has approximately 22% of the world’s coal reserves. Since 1961, the electric power sector has been the major user of coal in the U.S., accounting for 91.9% of the coal consumed in the country in 2021. Sixty-two percent of total U.S. coal production in 2021 was sourced from surface mining, and Wyoming, West Virginia, Pennsylvania, Illinois, and Montana were responsible for 74% of this total production.

Coal-mining activities expose sulfide minerals in rocks that, when in contact with oxidizing conditions, produce sulfate-rich drainage known as acid mine drainage (AMD). AMD treatment typically involves chemical treatments to raise …

Roles And Responsibilities Of A Coach Developer In A Youth Soccer Setting In The United States, Christina Ann Villalon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although coaching has a long history guided in the apprenticeship or mentorship model (Taylor & Garratt, 2013), research has primarily focused on the athlete, rarely on the coach, and almost never on whom the coach is serving as an apprentice to, or being mentored or supported by. Internationally, this role has been termed a ‘coach developer,’ but the formalized title and role of the coach developer is still a rather new concept (ICCE, 2014), with most research focused on the coach developer working at elite levels. Therefore, the purpose of this study was to explore the roles and responsibilities of …

Understanding Vaccine-Induced Memory Responses To Develop The Next Generation Of Pertussis Vaccines, Kelly L. Weaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

Bordetella pertussis is a Gram-negative respiratory pathogen that can infect individuals of all ages and is notably responsible for infant fatalities. Pertussis (whooping cough) is a vaccine-preventable respiratory disease for which vaccines have been available in the United States since the 1940s. Whole cell pertussis vaccines kept incidence of pertussis under 5,000 cases per year, but were associated with reactogenicity issues. Following the introduction of less reactogenic acellular pertussis vaccines in the late 1990s, there have been noteworthy outbreaks of pertussis. For example, in 2012 there were over 50,000 reported cases of pertussis and 20 deaths in the U.S. The …

An Action Research Evaluation Of An Online Inclusive Sport Peer Coach Training, Margaret Roberts Condon

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Trained peer tutors are a proven pedagogical technique that can help eliminate barriers to inclusion in physical education and extracurricular sport, including a reported lack of training and experience of professionals. When adequately trained, peer tutors can encourage social interactions, provide constant feedback, and increase active learning and engagement. While recommendations for training peer tutors have been provided, there are currently no known studies examining a peer tutor training in an online format for middle and high school-aged students participating in an inclusive sport program.

Purpose: The purpose of this study was to evaluate the effectiveness of an …

A Novel Tool To Promote Surgical Team Awareness Of Nurse Anesthesia Students’ Level Of Training, Joseph Lewis Britz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Communication errors in operating room (OR) settings are a common and avoidable source of patient harm. Communication in OR settings is enhanced when all members of the surgical team have knowledge of the skills and proficiency of their fellow team members. Student registered nurse anesthetists (SRNAs) provide anesthesia under the supervision of a certified registered nurse anesthetist (CRNA) or physician anesthesiologist. SRNAs’ proficiency in delivering anesthesia can vary significantly depending on their level of training, which varies based on the progression of the student in their multi-year nurse anesthesia program. Methods: This quality improvement project implemented a standardized non-verbal …

Love At First Profile: An Experiment Exploring If Previously Incarcerated Individuals Are Less Desirable While Online Dating, Carina L. Perrone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Incarceration is a rapidly increasing occurrence in the United States with more than 1.4 million people incarcerated and more than 2 million on some form of restricted confinement. While men are incarcerated at a much higher rate than women, women are still at a steady incline of incarceration as well. Also, people of color disproportionately make up a large number of those incarcerated while accounting for a small percentage of the general population. Previous research on people who have been incarcerated focuses on it affects education, employment, and marriage. Little research has attempted to explore how incarceration effects dating, a …

The Relationship Between Oral Health And Health-Related Quality Of Life Among Hemodialysis Patients In Rural West Virginia, Kimberly A. Wallace

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introduction: Approximately 786,000 Americans are currently diagnosed with end-stage renal disease, 71% of whom are undergoing hemodialysis (HD) treatment. There is a bidirectional relationship between chronic kidney disease and periodontal disease. Among HD patients, poor oral health is prominent and can negatively impact general health and health-related quality of life. Appalachian residents experience extreme health and poverty inequities and limited access to healthcare and dental providers.

Method: The study was designed based on the Andersen Behavioral Model of Health Services Use. 120 participants from 6 outpatient HD centers were enrolled. Data were collected using a 34-item questionnaire including oral health …

Tumor-Targeted Ph-Sensitive Manganese Oxide Nanoparticle For Enhanced Detection Of Breast Cancer Using Mri, Celia Martinez De La Torre

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, approximately 287,850 new cases of invasive breast cancer will be diagnosed, and over 43,000 women will die because of it in 2022. Currently, mammography is the gold standard for breast cancer surveillance. Although this detection technique is relatively cheap and fast, it misses about 1 in 5 breast cancers in younger women with dense breast tissue, causing a delay in treatment. Compared to mammography, magnetic resonance imaging (MRI) has superior soft-tissue contrast that elucidates structural differences within soft tissue to detect more breast cancers. However, MRI still misses

NEMO particles were produced by synthesizing manganese oxide …

Developing Teaching Adaptability In Pre-Service Teachers Using Practice-Based Teacher Education, Kyuil Cho

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to examine how pre-service teachers (PSTs) develop their teaching adaptive competence through teaching rehearsal and repeated teaching, which is one of the focused teacher education strategies in practice-based teacher education (PBTE). This study was conducted in an introductory teaching methods course of a physical education teacher education (PETE) program. A total of 22 PSTs participated in the study. Fourteen were male, and eight were female. The PSTs had varied coaching experiences ranging from little to no coaching in limited recreational sports settings, and none had teaching or coaching experience in the school setting.

A …

The Business Of Behavior: Appalachian Teachers’ Experiences In Using Positive Behavior Interventions And Support, Erin Michele Coffield

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores the lived experiences of Appalachian elementary and middle school teachers who have been identified as working in Positive Behavior Intervention and Support (PBIS) schools. This study used Lifeworld Theory to engage in discussion about the teachers’ individual experiences in using PBIS in their classrooms in relation to resilience frameworks and childhood trauma. The teachers’ individual experiences were analyzed using a Whole-Part-Whole analysis to bring the individual stories together to better understand how PBIS and childhood trauma are experienced in a specific Appalachian school. Discussion opens up through the participants' experiences about grit models in school, trauma their …

First Responders’ Assumptions: An Exploration Of Emergency Medical Technicians’ Secondary Traumatic Stress And Perceptions Of Substance Users, Glenn Noble

Graduate Theses, Dissertations, and Problem Reports (ETD)

Emergency medical technicians (EMT) and firefighters respond to critical incidents and provide emergent care. As the opioid crisis continues and drug overdose rates increase, first responders administer first aid to substance users, including Naloxone, more frequently. This study examined the influence of repeated exposure to drug-related critical incidents and provision of care to substance users on EMTs' and firefighters' secondary traumatic stress (STS) level, world assumptions, and substance use stigma. First responders' world assumptions, STS, burnout, and exposure to drug-related overdoses were analyzed for their power in predicting substance use stigma. Results suggest that occupational burnout, rather than STS, is …

Mechanistic Insight Into Chronic Stress-Induced Cerebrovascular Dysfunction, Emily Nicole Burrage

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite decades of research psychological stress remains a prevalent problem in society, often diminishing quality of life and increasing the risk of developing dementia. The brain plays a central role in the response to stress and as it is unable to store energy substrates, the brain relies heavily on dynamically regulated blood flow. Therefore, it is important that there is a tight coupling between neural activity and cerebral blood flow to meet metabolic demands and maintain critical brain function. It has been established that chronic psychological stress has detrimental effects on cerebrovascular function, potentially through increased superoxide anion production through …

It’S A Bleed: Pediatric Hemophilia And Length Of Stay, Rural Vs Urban Hospitals, Daniel G. Liedl

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hemophilia is a rare genetic disorder that requires specialty care and treatment. Pediatric patients with hemophilia have unique medical issues that may lead to permanent disability or death if not properly diagnosed and treated in a timely manner. Due to lack of resources and proper training of staff, rural hospitals are not equipped to properly treat pediatric hemophilia patients. Utilizing the Healthcare Cost and Utilization Project (HCUP) Kids´ Inpatient Database (KID) of the Agency for Healthcare Research and Quality. I have found, across all hospital types, pediatric hemophilia patients have longer lengths of stay, 2.7 days for rural hospitals, 4.6 …

An Investigation Into The Mechanism Of Proteasome Dysfunction In Neurodegenerative Disease And The Biological Impact Of Proteasome Hyperactivation In C. Elegans, Raymond T. Anderson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Aging is an inevitable process that occurs as humans grow older. It is characterized by the chronological accumulation of cellular damage over time leading to functional decline as an organism grows older. Several processes are thought to contribute to the aging phenomenon, but one of the most prolific of these is the disruption of protein homeostasis (proteostasis). The collapse of proteostasis can lead to accelerated aging and the development of age-related diseases including devastating neurodegenerative diseases (NDs) like Alzheimer and Parkinson disease. Virtually all NDs are characterized by the buildup of proteins in and around neurons resulting in neuronal death …