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Non-Target Analyses Of Novel Psychoactive Substances; Inherent Variation In Gcms Relative Abundances And Gas Phase Hydrogen Deuterium Exchange Isomer Discrimination, Kristin Michelle Kelly

Graduate Theses, Dissertations, and Problem Reports (ETD)

Novel psychoactive substances (NPSs) are synthetically derived compounds designed to mimic the effects of other illicit drugs. An endless cycle of NPSs continually reach the drug market due to limitations in drug legislation creating two problems: (1) non-availability of standards and (2) multiple isobars indistinguishable even to high resolution mass spectrometry. Gas chromatography-mass spectrometry (GCMS) is the most common instrument used for compound identification in non-targeted seized drug analysis. Compound identification using this technique relies on mass spectra where the percent relative abundances (%RAs) of all m/z values are compared manually or searched against a library database. Lacking standards, laboratories …

Three Essays On The Economics Of Public Libraries, Amir Borges Ferreira Neto

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation explores empirical issues concerning public libraries in the United States. In particular, it analyzes the direct and indirect effects to and from public libraries, on different issues such as charitable donations, labor market outcomes, institutions and technical efficiency. Chapter 1 discusses the economics of public libraries and its relevance. In addition, it provides an outline of the dissertation and its main contribution to the literature. Chapter 2, analyzes the determinants of donations to public libraries testing the crowding effect from government funding of public libraries. I find suggestive results of a crowd in effect with an inverted U …

Aptamer Functionalized Zinc Oxide Field Effect Transistors For Odor Detection, Michael D. Aldridge

Graduate Theses, Dissertations, and Problem Reports (ETD)

Odor detection and identification are complex processes, and tasks that currently only animals do well. There is a pressing need for an electronic nose, or eNose, with good sensitivity, selectivity, and speed that mimics that ability. Food quality control operations, environmental sensing, occupational safety, and the defense sectors all require systems that can rapidly and reliably detect trace levels of volatile organic compounds. The goal of this work is to create a biologically inspired device which can accurately detect and identify odors at concentrations consistent with the most sensitive biological systems.

In order to mimic a natural olfactory system, we …

In Vitro Metabolism Of The Synthetic Cannabinoids Px-1, Px-2, Px-3 And A Comparison Of Their Clearance Rates In Human Liver Microsomes, Travon Cooman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Detection of SCs in body fluids continue to be a challenge because of limited metabolism data, lack of standards and reference mass spectrometry data. In vivo and in vitro experiments help elucidate metabolite markers for novel psychoactive substances and can prompt synthesis of standards to verify proposed metabolites. In this study, metabolism of three SCs N-(1-amino-1-oxo-3-phenylpropan-2-yl)-1-(5-fluoropentyl)-1H-indole-3-carboxamine (PX-1), N-(1-amino-1-oxo-3-phenylpropan-2-yl)-1-(5-fluropentyl)-1H-indazole-3-carboxamide (PX-2), and N-(1-amino-1-oxo-3-phenylpropan-2-yl)-1-(cyclohexylmethyl)-1H-indazole-3-carboxamide (PX-3) were investigated using human liver microsomes. Previous studies showed PX-3 as the most potent CB1 and CB2 receptor agonist.

Half-life and clearance data were acquired using liquid chromatography- tandem mass spectrometry. Metabolite elucidation was performed using liquid chromatography …

Impacts Of Climate Change On Water Balance Components In The Central Appalachian Mountains, Usa, Brandi Anne Gaertner

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research investigated the impact of climate and land cover on water balance components including evapotranspiration and runoff in the mountainous central Appalachian region of the United States. The first studyanalyzed trends in climatologic, hydrologic, and growing season length variables, identified the important variables effecting growing season length changes, and evaluated the influence of a lengthened growing season on increasing evapotranspiration trends. The results showed that growing season length has increased, on average, by ~22 days and evapotranspiration has increased ~12 mm. The second study quantified long-term historical and future climate trends, evaluated water balance sensitivity to change, and quantified …

Material Disease: Agency And Illness In Early American Literature, Mariah Crilley

Graduate Theses, Dissertations, and Problem Reports (ETD)

Material Disease: Agency and Illness in Early American Literature argues that diseases shaped bodies of literature. Drawing on new materialism, disability studies, and the medical humanities, this dissertation argues that diseases were material and literary agents that transformed not only individual bodies and lives but also cultures, history, and even literature. In each chapter, I explore how one disease impacted a particular genre or paradigm: syphilis and the natural history, smallpox and the body politic, yellow fever and the novel, dysentery and sensibility, and malaria and narratives of Western expansion. By emphasizing disease’s material and literary agency, by conjoining the …

On Matching Faces With Temporal Variations Using Representation Learning, Daksha Yadav

Graduate Theses, Dissertations, and Problem Reports (ETD)

Developing automatic face recognition algorithms which are robust to intra-subject variations is a challenging research problem in the computer vision research community. Apart from the well-studied covariates such as pose and expression, temporal variations in the facial appearance also lead to a decline in the performance of face recognition systems. This research focuses on analyzing the temporal variations in facial features due to facial aging, facial plastic surgeries, and prolonged illicit drug abuse. The contributions of this dissertation are fivefold: (i) behavioral and neuroimaging studies are conducted to understand the human perception of faces affected by temporal variations, specifically facial …

Analysis Of User Preference With N95 And Powered Air-Purifying Respirators In A Healthcare Work Environment, Brittany Renee Carver

Graduate Theses, Dissertations, and Problem Reports (ETD)

For those working in the healthcare field, personal protective equipment is vital. Healthcare workers are often first responders when it comes to dealing with patients who are infected or when a disease outbreak occurs. The Occupational Safety and Health Administration (OSHA) requires all healthcare operations to provide employee protection against blood borne pathogens. With the increase of life-threatening disease outbreaks, such as SARS in 2003 and Ebola in 2014, it is of the upmost importance to make sure respirators are best suited for users and acceptable for long term use. It has been shown through previous studies that due to …

Regulation Of The Long Non-Coding Rna Fam83h-As1 By Human Papillomavirus In Cervical Cancer, Jamie Ann Barr Ph.D.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Non-coding RNAs (NcRNAs), such as long non-coding RNAs (lncRNAs) and microRNAs (miRNAs), have been found to be involved in a variety of critical biological processes, and dysregulation of ncRNAs have been involved with several human diseases including cancer.

High-risk human papillomavirus (HPV) infection is one of the first events in the process of carcinogenesis in cervical and a subset of head and neck cancers. The expression of the viral oncoproteins E6 and E7 is essential in this process by inactivating the tumor suppressor proteins p53 and Rb, respectively, in addition to their interactions with other host proteins and regulation of …

Probability Discounting Of Lewis And Fischer 344 Rats: Strain Comparisons At Baseline And Following Acute Administration Of D-Amphetamine, Jenny E. Ozga

Graduate Theses, Dissertations, and Problem Reports (ETD)

Risky choice can be defined as choice for a larger, uncertain reinforcer over a smaller, certain reinforcer when choosing the smaller alternative maximizes reinforcement. Risky choice is studied using various procedures in the animal laboratory; one such procedure is called probability discounting. There are many variables that contribute to risky decision-making, including biological and pharmacological determinants. The present study assessed both of these variables by evaluating dose-response effects of d-amphetamine on risky choice of Lewis (LEW) and Fischer 344 (F344) rats. The probability-discounting procedure included discrete-trials choices between one food pellet delivered 100% of the time and three food …

Development Of A Task Analysis Tool To Estimate Shoulder Strain During A Lifting Task, Christopher Wayne Moore

Graduate Theses, Dissertations, and Problem Reports (ETD)

Musculoskeletal disorders of the shoulder have a huge impact on the workplace and employees. Due to the complexity and high mobility of the shoulder, developing a task analysis tool with a focus on the shoulder is difficult, and while there are some ergonomic analysis tools that do consider the shoulder to varying degrees in the analysis, none focus purely on the shoulder. Therefore, this research was undertaken in an attempt to develop a shoulder-based task analysis tool for the shoulder. However, since the scope of work involving the shoulder is so vast, the tool in this research focuses on unobstructed, …

Supporting The Injured Athlete: Coaches’ Perspectives On Providing Social Support, Stefanee Opal Maurice

Graduate Theses, Dissertations, and Problem Reports (ETD)

Injury is an inevitable facet of sport participation, and injured athletes require support from coaches. However, research on injured athletes highlights a lack of support from coaches. Building on the conceptual model proposed by Maurice et al., this study uses the International Sport Coaching Framework (ISCF) to examine ways contextual coaching knowledge is used to support athletes throughout rehabilitation. Previous research has focused on the knowledge types but has neither addressed the integration of the knowledges in a single study nor examined them in an injury context. A generic qualitative approach was used to examine 13 NCAA DI coaches’ perception …

Numerical Modeling Of Hydraulic Fracture Propagation And Comparison With Microseismic Data At A Field Site, Carter Lawrence Hulcher

Graduate Theses, Dissertations, and Problem Reports (ETD)

A hydraulic fracturing site in Morgantown, West Virginia, USA was selected to be a research site for the Marcellus Shale Energy and Environment Laboratory (MSEEL) project which was funded by the U.S. Department of Energy (DOE). The overall objective of the MSEEL research project is to evaluate and improve technologies to enhance shale gas recovery with minimal impacts on the environment. The field site has two previously drilled wells and two newly drilled wells for extracting natural gas. A separate exploratory well (or “science well”) was also drilled and includes a geophone array to extract important seismic/microseismic event information, which …

Ground Ingredients: Analysis Of Lead Exposure In The California Condor’S (Gymnogyps Californianus) Ground Foraging Habitat, Evan Michael Mcwreath

Graduate Theses, Dissertations, and Problem Reports (ETD)

The California Condor (Gymnogyps californianus) is a critically endangered species that reached its nadir in 1987 with a population of 27 birds in the wild. Intensive management efforts have been implemented to aid the condors’ recovery, however, anthropogenic factors, like use of lead ammunition, continue to cause fatalities in this vulnerable population. Lead toxicosis, which is responsible for approximately 40% of all condor deaths since 1992, is one of the most significant threats to condors. In birds lead poisoning leads to neurological dysfunction, reproductive impairment, immune suppression, gastrointestinal disturbance, anemia, and ultimately increased vulnerability to predation, starvation, and infection. For …

Lead Exposure Of North American Raptors, Vincent A. Slabe

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lead is an anthropogenic threat to terrestrial wildlife, has no physiological benefits, and is considered a neurotoxin. Legislation adopted in many developed countries restricts the uses of lead in consumer and industrial products such as pipe fittings, petrol, paint, and shot for use in waterfowl hunting. However, lead is currently categorized by the United States (US) government as a critical metal and common uses of lead still persist in North America. These include the use of rifle ammunition, shot in upland gamebird hunting, and fishing sinkers. Lead is also a by-product of multiple industrial operations including smelters, coal power plants, …

The Effects Of Obesity, Chronic Stress, And Exercise On Pancreatic Health, Abigail Tice

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obesity is concomitant with hyperglycemic conditions, increased proinflammatory cytokines, and increased oxidative stress. Under hyperglycemia, thioredoxin interacting protein (TXNIP) is increased and causes increased oxidative stress and pancreatic b-cell apoptosis. Chronic stress is associated with increased glucocorticoid levels and a proinflammatory environment. The purpose of this study was to examine the effects of obesity, chronic stress, and exercise on pancreatic health.

Lean Zucker Rats (LZRs) and Obese Zucker Rats (OZRs) were randomly assigned to four groups: 1) sedentary controls (C: n=8 per); 2) unpredictable chronic mild stress (UCMS) (S: n=8); 3) exercise (E: n=8); and 4) combined UCMS and exercise …

Development Of A Dynamic Model And Control System For Load-Following Operation Of Supercritical Pulverized Coal Power Plants, Parikshit S. Sarda

Graduate Theses, Dissertations, and Problem Reports (ETD)

Thermal power plants that have been designed to operate at their rated capacity are being forced to cycle their load and operate under low-load condition to meet changing load demands due to the increased penetration of renewables into the electric grid. The rapid load-following operation is leading to challenging control problems. The goal of this research is to develop dynamic model and control system for efficient load-following operation. The focus of this work is on supercritical pulverized coal (SCPC) power plants. The steady-state model is developed using Aspen Plus and Aspen Custom Modeler and then converted to a pressure-driven Aspen …

Acute And Repeated Effects Of Synthetic Cannabinoid Agonism And Cannabinoid Receptor 1 Positive Allosteric Modulation, Kristen R. Trexler

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent years have seen a rise in the diversity and use of synthetic cannabinoids. Currently, there is little known about the effects of specific synthetic cannabinoid compounds. As such, little research has been done evaluating the acute and chronic effects of synthetic cannabinoid administration or the development of tolerance and withdrawal. The present study aimed, in part, to evaluate the acute and repeated effect of a third-generation synthetic cannabinoid, AB-FUBINACA. Mice were treated with AB-FUBINACA (0.1-3 mg/kg, i.p.) or vehicle and were tested repeatedly in the tetrad battery of assays, which included tests of catalepsy, antinociception, hypothermia, and locomotor activity. …

Movement In The Classroom: Contingent Factors Underlying Teacher Change, Annie E. Machamer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Teachers' use of classroom physical activity (PA) has been found to significantly impact students PA participation, time on task, and academic performance. However, teachers have continued to identify barriers affecting delivery related to professional development opportunities, learning environment, and experiences in practice. An understanding of the connection between these experiences and their impact on teacher change is needed.

Purpose: The purpose of this study was to investigate teachers’ perceptions of specific factors that have influenced their continued implementation of classroom PA. This study provides insight into the teacher perceptions, and aims to identify connections among orientation to learning and …

The Association Of Attitude, Perceived Behavioral Control, And Intention With Fruit And Vegetable Intake Among Rural West Virginian Residents With Pre-Diabetes In Mineral And Hampshire Counties, Diana Leigh Niland

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Association of Attitude, Perceived Behavioral Control, and Intention with Fruit and Vegetable Intake among Rural West Virginian Residents in Mineral and Hampshire Counties with Pre-diabetes

Introduction: Pre-diabetes affects approximately 84.1 million adults in the United States. Pre-diabetes is one of the leading predictors of type 2 diabetes. West Virginia ranks number one in the prevalence of diabetes. Fruit and vegetable intake may assist in reducing risk for pre-diabetes. However, most adults do not consume the recommended daily amount. There are no studies investigating the fruit and vegetable intake (FVI) of rural West Virginian residents with pre-diabetes.

Method: After IRB …

Cognitive And Affective Influences On Decision-Making Strategies And Outcomes, Michaela S. Reardon

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines how factors such as emotion and planning abilities, determine decision-making strategies and outcomes. Consumer-based decision tasks are one way for researchers to measure the decision-making process and outcomes of individuals, while bringing an element of reality to the task through the utilization of decisions about everyday items that someone might purchase (e.g., a car, apartment, etc.). Using these types of tasks, researchers can measure the quality of a decision (e.g., did the participant come up with the best solution?), as well as the decision or search strategy. Previous research shows that cognitive factors are important when individuals …

Promoting Sense Of Belonging And Interest In Geosciences Among Undergraduate Women Through Mentoring, Wenyi Du

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study is to investigate whether students’ university sense of belonging mediates the relationship between mentor network diversity and students’ interest development among undergraduate women in STEM majors. The sample for this study was consisted of 277 undergraduate women majoring in a STEM discipline with initial interests in geosciences across nine universities within the United States. A regression-based mediation analysis was performed using the Hayes’ (2013) macro to test the indirect effect of mentor support on interest through university sense of belonging. Consistent with our hypothesis, the analysis revealed a statistically significant and positive indirect effect of …

In Situ Measurement Of Fatigue Induced Crack Growth In Inconel 718 Using Direct Current Potential Drop Method, Joel C. Lindsay

Graduate Theses, Dissertations, and Problem Reports (ETD)

With recent advances in air breathable engines comes more extreme temperature environments that engine components must tolerate. During the design of these engines, it is necessary to understand how material fatigue failures occur at these new, higher operating temperatures. In providing understanding, the following fundamental study focuses on the statistical nature of crack jumps (changes in crack length over time) during fatigue in a polycrystalline nickel-based superalloy, Inconel 718 (IN718). In situ measurement of the crack length at several loading conditions were conducted using a direct current potential drop (DCPD) measurement method. Experimental data was collected at six different fatigue …

Health Literacy And Family Factors In The Transition To Adult Care In Adolescents With Type I Diabetes, Ellen M. Manegold

Graduate Theses, Dissertations, and Problem Reports (ETD)

Successful management of type 1 diabetes (T1DM) in adolescence involves multiple daily tasks, developmental changes, and the expectation of transition to an adult provider. Health literacy is one variable to consider in the context of transition, as studies have demonstrated the correlation of parental health literacy with health behaviors and outcomes, yet not studied adolescent health literacy in T1DM. Family factors (e.g., management responsibility, diabetes-specific family conflict, parental support) also are important to behavior and health outcomes in adolescents with T1DM. Study aims were to: 1) examine the association of adolescent health literacy to transition readiness and health outcome in …

Stockmanship Education And Evaluation, John K. Yost

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation documents two separate works. The first is an evaluation of WVU SFMS/SOIDC Large Animal Module and the second is the development and use of the Stockman’s Scorecard. The WVU SFMS/SOIDC Large Animal Module provides foundational information on food animal husbandry and veterinary procedures to SOF Combat Medic candidates. A quasi-experimental design was used to determine if the module content resulted in an increase in food animal production knowledge for the participants. Seventy-five percent of the subjects had no previous livestock exposure and only seven percent had previously participated in 4-H or FFA. Matched pair analysis determined that the …

Following The Leaders: Issue Attention And Agenda Dynamics In Women’S Health Care Policy, Kara Anne Fisher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation focuses on shifts in issue attention in the policy process and examines policy changes. Describing agenda setting is important not only for understanding congressional behavior in general but also for understanding the institutional context of other political behavior. I focus on the processes of positive feedback to explain periods of dramatic policy changes observed over a long period of time.

The purpose of this study is to examine the changes in the politics of health policy which opened the door to women’s health care as an important feature of health politics. Thus, my research is motivated by three …

Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pregnancy loss in cattle is greatest in the first 30 days of gestation. Near d15 of gestation, bovine conceptuses elongate and express LGALS1, an immunosuppressive glycan-binding protein believed to be essential for implantation. However, other members of the bovine galectin family, including LGALS3, LGALS4, LGALS7, LGALS8, LGALS9 and a galectin-13-like gene (LGALS13L), also may be expressed by the elongating conceptus. Therefore, galectin expression in elongating, d16 bovine conceptuses and intercaruncular endometrium as well as elongating, d14 ovine conceptuses and intercaruncular endometrium was assessed by RT-qPCR. Angus heifer and Texel ewes underwent estrous cycle synchronization and either inseminated or not bred. …

Internalized Heterosexism, Outness, Athletic Identity, And Perceived Stress Among Queer Female Athletes, Olivia C. Scott

Graduate Theses, Dissertations, and Problem Reports (ETD)

The historical presence of heterosexism in sport has been discussed in numerous publications, with unique considerations for female athletes (Anderson, Magrath, & Bullingham, 2016; Cahn, 1993; Griffin, 1998; Rankin, 1998). Unfortunately, few studies have examined the impact of this climate on female athletes with minoritized sexual identities. Most research pertaining to this population has been qualitative and largely focused on lesbian athletes (Fynes & Fisher, 2016; Stoelting, 2011). Consequently, more generalizable conclusions about queer female athlete experiences has been limited. The present study was an attempt to address this research gap, by assessing the internalized heterosexism, outness, athletic identity, and …

Trustworthiness And Stability In Same And Different Sex Relationships: Heterosexuals’ Attitudes Toward Bisexuality, Jeneice Shaw

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although bisexuals are reportedly the largest sexual orientation minority group in North America (Copen, Chandra, & Febo-Vazquez, 2016), there is scant research examining the population without also including lesbian women and gay men. However, according to the American Psychological Association’s (2012) Guidelines for Psychological Practice with Lesbian, Gay, and Bisexual Clients, psychologists should endeavor to understand the unique experiences of the bisexual population. Bisexual individuals face different stereotypes, conflict within the LGB community, and different life experiences related to other sexual orientations (Rust, 2000). Two unique stereotypes applied to the bisexual community are that bisexuality is an unstable sexual orientation …

Differences In Sexual Delay Discounting Among In-Treatment Adults With Opioid Use Disorder, Jonathan J.K. Stoltman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Previous research has found impulsive decision-making to be a core component of addiction (Moody, Franck, Hatz, & Bickel, 2016). One way to measure impulsive choice is through the use of a delay discounting task. The delay discounting task provides a way to measure choice of immediate, smaller rewards compared to delayed, larger rewards (Odum, 2011b). An emerging area of research in addiction science is the intersection of addiction and sexual health. Previous sexual delay discounting research has focused on whether attractiveness or STD risk can shift the likelihood of waiting until a condom was available (Johnson & Bruner, 2012). This …