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Initial And Advanced Stages Of Microbiota Establishment Within The Tsetse Fly, Miguel Eduardo Medina Munoz

Graduate Theses, Dissertations, and Problem Reports (ETD)

Symbiosis is a long-term physical association between two or more species, although little is known regarding its evolutionary origins, particularly at the genetic level. Tsetse flies are the vector of African trypanosomes, causative agents of Human and Animal African Trypanosomiases. Tsetse provide an ideal model for studying initial and advanced stages of symbiosis. Tsetse have a simple digestive tract microbiota primarily consisting of two bacteria; the ancient mutualist Wigglesworthia glossinidia and the recently acquired Sodalis glossinidius. This work presents a chronological study in evolutionary terms of the history of a microbial-insect association. First, I present concepts on symbiosis and …

Bypassing The Blood-Brain Barrier: A Physical And Pharmacological Approach For The Treatment Of Metastatic Brain Tumors, Samuel A. Sprowls

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided an in depth literature review of methods to disrupt the BBB/BTB and improve therapeutic distribution to brain tumors, (b) evaluated the use of azacitidine as a single agent therapy for the treatment of brain metastasis of breast cancer and a potential molecular mechanism by which brain tropic cells are sensitized to hypomethylating agents, (c) determined the impact cannabidiol has on P-glycoprotein mediated efflux at the blood-brain barrier and its potential for use as a single agent treatment for metastatic brain tumors, (d) developed a preclinical radiation therapy protocol for use in small animals and in vitro …

Determination Of Effectiveness Of Energy Management System In Buildings, Vivash Karki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Building Energy Management Systems (BEMS) are computer-based systems that aid in managing, controlling, and monitoring the building technical services and energy consumption by equipment used in the building. The effectiveness of BEMS is dependent upon numerous factors, among which the operational characteristics of the building and the BEMS control parameters also play an essential role. This research develops a user-driven simulation tool where users can input the building parameters and BEMS controls to determine the effectiveness of their BEMS. The simulation tool gives the user the flexibility to understand the potential energy savings by employing specific BEMS control and help …

Differences In Smoking Behaviors And Readiness To Change For Patients With Copd And Differing Categories Of Depression, Jessica M. Floyd

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Chronic obstructive pulmonary disease (COPD) is the third leading cause of death in the world. Depression is a common comorbidity of COPD and affects patient outcomes and smoking cessation. The purpose of this study was to explore and describe factors influencing smoking behaviors and readiness to change in patients with comorbid COPD and depression.

Aims: The research question that guided this study was, “What are the relationships among smoking, COPD, and depression?” The following aims stemmed from the research question: to describe the characteristics of a convenience sample of people living with COPD and differing categories of depression who …

Making Sense Of And Giving Sense To Management Research On Social Evaluations And Ethical Decisions: A Three Essay Dissertation, Alanna M. Hirshman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Organizations, whether private or public, are subject to evaluations by their stakeholders and society. While positive social evaluations can help organizations build their reputation and social capital, negative evaluations can cause significant social and economic distress. In both cases, stakes are high for organizations. Thus, understanding how social evaluations are formed and taken into account during firms’ strategic decision-making is paramount. This three-essay dissertation builds on the sensemaking and sensegiving literature in order to explain positive and negative social evaluations in the context of ethical decision-making. Specifically, the following three questions are addressed: a. Which types of sensemaking and sensegiving …

Evaluation Of Population Structure, Age, Growth, And Mortality Of Blue Catfish And Flathead Catfish Within The Robert C. Byrd Pool Of The Ohio And Kanawha Rivers, Joseph Vincent Siegel

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Ohio River is continuing to recover from a long history of anthropogenic impacts. Blue Catfish were considered locally extirpated from the West Virginia sections and have been reintroduced through fingerling stockings beginning in 2002. Flathead Catfish are naturally sustaining in the pool and combined with Blue Catfish provide two popular recreational fisheries. The Blue Catfish and Flathead Catfish studies shared similar objectives: (1) evaluating size structure, (2) estimating growth rates, and (3) estimating annual mortality. From 2017 to 2020, we collected catfish annually through low frequency electrofishing and trotline surveys at 10 fixed locations. Electrofishing catch rates for Blue …

The Effects Of Dementia Knowledge On Dementia Worry, Attitudes, Social Comfort, Empathic Concern, And Personal Distress, Alexandria Rose Ebert

Graduate Theses, Dissertations, and Problem Reports (ETD)

The present study examined the effects of different types of dementia knowledge on attitudes and affective reactions towards persons with dementia. Work has demonstrated that individuals with higher levels of personhood-based knowledge have lower levels of personal dementia fear and higher levels of social comfort. However, to our knowledge, work has not examined dementia attitudes more broadly or differentiated causal relations among different forms of dementia knowledge and attitude outcomes. Participants (N = 334) aged 19-78 (M = 44.53, SD = 16.57) were randomized into one of five experimental conditions: (1) biomedical-knowledge (BK; read biological and medical facts about …

Implications Of Fraud Detection Decisions, Marie Rice

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is comprised of three studies that examine the implications of fraud detection decisions. The first study examines whether auditors’ mindfulness practices influence their ability to accurately detect fraud risk factors. The second study evaluates whether organization type influences whether and how perpetrators are punished. The third study evaluates whether management’s decision to increase controls when fraud occurs has unintended negative consequences for other employees. Together, these studies contribute to accounting literature related to fraud detection.

Employing Exoglycosidase And Transferase Enzymes In Capillary Nanogel Electrophoresis For The Determination Of N-Glycan Linkages And Enzyme Michaelis-Menten Constants., Lloyd Bwanali

Graduate Theses, Dissertations, and Problem Reports (ETD)

As a post translational modification protein glycosylation plays a crucial role in protein signaling, binding, kinetics and folding. In disease diagnosis and prognosis, monitoring glycosylation has been identified as a biomarker. Sialylation and sialic acid linkage in N-glycans are markers of cancers including liver, pancreatic and kidney cancer. Quantification of sialic acid linkage is analytically challenging because of the diverse linkages and the presence of heterogenous branching. A capillary electrophoresis method is reported that integrates a unique combination of enzymes and lectins to modify sialylated asparagine-linked glycans (N-glycans) in real time in the capillary so that N-glycan structures containing α2–6-linked …

Effect Of Fecal Egg Count Estimated Breeding Value On Antibody Production In Katahdin Lambs, Kelsey L. Bentley

Graduate Theses, Dissertations, and Problem Reports (ETD)

With current pressures to seek out genetic solutions to combat antimicrobial resistance, genetic resistance to the gastrointestinal nematode Haemonchus contortus may reduce need for prophylactic antibiotic use. The Katahdin breed has effectively utilized post-weaning fecal egg count estimated breeding value (PFEC EBV) to reduce parasitism within their flocks, but there still remains significant opportunity for selection within breed. In a previous study, Katahdin sheep sired by low PFEC sires had greater survivability to weaning than lambs sired by high PFEC rams and preweaning was caused by other diseases than parasitism. Those data lead to the hypothesis that selection for PFEC …

Tree Species Influence On Microbial Functional Gene Abundance And N Cycling In Riparian Soil, Elizabeth Matejczyk

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about how above-ground vegetation may differentially influence the below-ground microbial community structure, abundance, and function. Abundance of soil microbial N cycling genes responsible for nitrification (amoA), denitrification (nirK and nirS), and nitrous oxide reduction (nosZ) may vary with tree species and increasing N availability, and these variables may be used to predict production of NO₃^- and N₂O from soil. Variability of nitrification and denitrification rates have also been linked to tree mycorrhizal associations, with soil beneath tree species associated with arbuscular mycorrhizal (AM) exhibiting greater denitrification rates …

Soil Microbial Nitrogen-Transforming Genes Influenced By Tree Species And Fertilization In An Appalachian Hardwood Forest, Emma G. Fox-Fogle

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many forest ecosystems have been receiving elevated nitrogen (N) deposition due to human activity. Increased ecosystem N threatens soil fertility, water and air quality, and modifies the soil microbially-mediated N cycle in complex ways. The current study investigates how the abundance of key functional N cycling genes within soil bacteria is altered by N deposition and further mediated by different dominant tree species at the Fernow Experimental Forest, WV. Soils were analyzed from two watersheds, where watershed 3 (WS 3) has been receiving experimental applications of N fertilizer that are 2x historic ambient N deposition and 5x the current ambient …

Analysis On Combined Heat And Power, And Combined Heat And Power Hybrid Systems For Unconventional Drilling Operations, Diego G. Dranuta Ferrer

Graduate Theses, Dissertations, and Problem Reports (ETD)

The United States (U.S.) has experienced a natural gas “boom” due to the development of unconventional shale plays, but well development is energy intensive. Operations use electric drilling rigs typically powered by either three high-horsepower diesel engines (HHPDE) or three dedicated natural gas engines (DNGE) and associated generators. From a first law analysis, HHPDEs peak at 42% efficiency at full load, while DNGE peak at about 30%. Most of the fuel energy is lost as heat rejected by the exhaust and radiators. Concurrently, during cold seasons rigs utilize boilers to provide steam throughout the rig to prevent freezing and provide …

Women, Water, And Well-Being: Gendered Experiences Of Household Water Contamination In Parkersburg, West Virginia, Emily Brooke Tingler

Graduate Theses, Dissertations, and Problem Reports (ETD)

DuPont Washington Works chemical plant knowingly pumped hundreds of thousands of pounds of toxic C8 waste into local waterways, water tables, and landfills for over 40 years, contaminating the drinking water for more than 100,000 residents in and around Parkersburg, West Virginia. Drawing on feminist political ecology and the political ecology of water with literature on perceptions of risk in contaminated communities, environmental reproductive justice, modern water, and hydrosocial waterscapes, I examine, through a qualitative case study, the lived experiences of women who have an intimate understanding of C8 water contamination from the DuPont Washington Works chemical plant. Specifically, I …

The Effects Of Genetic Differences In An Innate Immune Receptor On Immune Responses To Haemonchus Contortus In Sheep, Denzel Deshawn Middleton

Graduate Theses, Dissertations, and Problem Reports (ETD)

Little is known about differences observed in early immunological signaling events that result in cellular infiltration and Th2 cytokine production that mediate parasite expulsion. Parasite resistant St. Croix (STC) sheep have been used as model for full host protective immunity as they present the ability to respond to larval stages of H. contortus. RNA sequencing analysis of peripheral blood mononuclear cells (PBMC) stimulated with H. contortus larval antigen (HcLA) revealed significant upregulation in NLRP3 and its pathway component TLR4. Predicted protein structural software identified distinct differences in NLRP3 protein structure in STC and parasite susceptible Suffolk (SUF) sheep. NLRP3 …

Energy Policy Dynamics In The European Parliament And In The Lithuanian Seimas, Vaida Lilionyte Manthos

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study is to apply punctuated equilibrium theory to better understand the development of energy policies in the European Parliament and in the Lithuanian Parliament – Seimas. In the first empirical chapter I analyze written questions that members of the European Parliament submitted to the EU Commission to study whether members of the European Parliament respond to punctuating events such as the Fukushima nuclear accident. I also study party groupings in the European Parliament to evaluate how punctuating events alter the policy behavior and priorities of these party groupings. In the second empirical chapter I analyze whether …

Examining West Virginia's Economic Development: Natural Resources, Development Agencies, And Labor Force Development, Gaillynn Marie Bowman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Examining West Virginia's Economic Development: Natural Resources, Development Agencies, and Labor Force Development Gaillynn Bowman Economic development is well-recognized as being fundamental to facilitating an overall improved quality of life for communities and their residents. Throughout West Virginia’s history, the state has experienced economic hardships caused by boom and bust cycles associated with resource extraction. This dissertation consists of three essays that explore the impacts of economic development activities, including conservation programs, economic development agency initiatives, and workforce development agencies. The first essay explores the relationship nonresident landowners have with the conservation programs within West Virginia, specifically the Natural Resources …

Effects Of 4-Methylcyclohexanemethanol On Stress Response Pathway Regulators, In Saccharomyces Cerevisiae, Suk Lan Ser

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hydrotropes are small molecules capable of inducing liquid-liquid phase separation by altering the solubility and conformation states of organic compounds that are increasingly becoming important in organizing chemical reactions and regulating complexes. They prevent protein aggregation causing these proteins to form condensates. Mediator, a highly conserved multi-subunit complex, plays an important role in transcription. Med15, a subunit found within the tail domain of the Mediator complex, works with stress-induced transcription factors and is regulated by many kinases, including CDKs and the AMP kinase, Snf1. Living cells respond by changing molecular and cellular pathways when they are exposed to stressful conditions. …

Recovery Of Phosphorus From Florida Phosphatic Waste Clay, Amir Eskanlou

Graduate Theses, Dissertations, and Problem Reports (ETD)

This MS thesis examines the recovery of phosphorus (P) from Florida waste clay (WC). A comprehensive literature review revealed that: (i)-The most important values being lost to WC are P and rare earth elements (REEs). For the recovery of these values from WC, two crucial attempts are the removal of extremely fine-sized clays, followed by the recovery of phosphate content, which can pave the path for the recovery of REEs; (ii)-Any scientific/ technological solution should, at the same time, be economically and environmentally attractive to the industry. As such, moving from mostly chemical separation processes to the primarily physical/ physicochemical …

Novel Mechanisms Of Neuronal And Hormonal Regulation Of Large Intestinal Electrolyte Transport, Andrew John Nickerson

Graduate Theses, Dissertations, and Problem Reports (ETD)

The mammalian colon plays a vital role in maintaining overall electrolyte and fluid homeostasis in the body, and it possesses the ability to either secrete or absorb water depending upon the needs of the organism. Secretion or absorption of electrolyte ions, such as Na⁺, Cl^- and K⁺, drives the movement of water across the colonic epithelium – the inner gut lining where all transport takes place. Aberrant absorptive and/or secretory activity within the colonic epithelium is central to the pathogenesis of many gastrointestinal diseases, such as irritable bowel syndrome (IBS), inflammatory bowel disease (IBD), constipation …

Grouping Algorithms For Informative Array Testing In Disease Surveillance, David Sokolov

Graduate Theses, Dissertations, and Problem Reports (ETD)

In order to maintain normal operations and prevent unnecessary morbidity and mortality during times of disease outbreak, institutions find a need to conduct frequent and widespread testing of their constituents, often under significantly limited testing resource constraints. Faced with the challenge of how best to allo- cate these limited resources to maximum effect, institutions are increasingly turning to group (or “pooled”) testing, which involves testing strategically-chosen groups of patient samples rather than individual samples, producing significant testing resource savings under certain regimes of disease prevalence. While group test- ing can be conducted without any a priori knowledge of individual disease …

Targeting Calcium Homeostasis For The Treatment Of Multiple Myeloma, Osama M. Elzamzamy

Graduate Theses, Dissertations, and Problem Reports (ETD)

Multiple myeloma (MM) is a hematological malignancy characterized by the neoplastic proliferation of the plasma cells. MM is a relatively rare cancer that accounts for about 1.8% of all cancers and is the second most common hematologic malignancies, and despite the advancement from untreatable to treatable malignancy, it is yet incurable. Calcium ions (Ca²⁺) play an important role as second messengers in regulating a plethora of physiological and pathological processes, hence cytoplasmic Ca²⁺ is tightly regulated with strict spatial and temporal control to initiate, maintain, and terminate appropriate signaling pathways and phenotypes including cellular proliferation, cell cycle …

Can Parent-Child Interaction Therapy Improve Core Features Of Autism? Findings From A Comparison Of Pcit For Children With And Without Autism Spectrum Disorder, Robin C. Han

Graduate Theses, Dissertations, and Problem Reports (ETD)

Although there is a small yet growing body of evidence supporting Parent-Child Interaction Therapy (PCIT) as an effective treatment for disruptive behaviors among children with autism spectrum disorder (ASD; Scudder et al., 2019), further study is warranted, particularly with more robust methodology (e.g., larger sample sizes, comparison groups). Furthermore, preliminary studies have demonstrated improvements in symptoms of autism following the completion of PCIT, including improvements in frequency of child verbalizations (Hansen & Shillingsburg, 2016), caregiver report of social skills and social responsiveness (Zlomke et al., 2017), time spent in pretend toy play (Lieneman et al., 2019), and shared positive affect …

Improving Real-Time Methane Monitoring In Longwall Coal Mines Through System Response Characterization Of A Multi-Nodal Methane Detection Network, Brian Philip Cappellini

Graduate Theses, Dissertations, and Problem Reports (ETD)

Machine automation and atmospheric monitoring are used by many industries to improve safety and productivity in the workplace. The underground Methane Watchdog System (MWS) is a multi-nodal network of sensors currently under development. The MWS aims to improve safety and productivity by introducing 10 compact sampling units designed to be easily integrated within the current roof support equipment of the mine. Each unit contains an array of sensors used to continuously monitor the environmental conditions which include methane concentration, temperature, pressure, and relative humidity. All MWS units report information back to a remote central processing hub (CPH) which collects nodal …

Decision-Making Involvement, Social Support For Healthy Behaviors, And Nutritional Adherence In Adolescents With Cystic Fibrosis, Jennifer Kelleher

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Adolescents with cystic fibrosis (awCF) exhibit suboptimal adherence to dietary and enzyme recommendations despite the fact that eating a high calorie diet with enzymes significantly improves lung functioning. Furthermore, during adolescence teens and their caregivers begin to negotiate treatment responsibility, shifting treatment tasks to the youth. As such, it is important to understand which aspects of the complex decision-making process (e.g., expressing an opinion, asking for information from parents/adults, making joint decisions with parents) relate to better dietary adherence in adolescents with CF and how parents can best support these healthy behaviors. Thus, this study aimed to identify how …

Characterization Of Modern Ammunition And Background Profiles: A Novel Approach And Probabilistic Interpretation Of Inorganic Gunshot Residue, Korina Layli Menking-Hoggatt

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gunshot residue (GSR) can provide essential clues in gun-related investigations. The standard practice for GSR analysis uses SEM-EDS, with the capability for single particle elemental and morphological analysis. However, the method is time-consuming and based on categorical classification models without considering case circumstances. Therefore, complementary and more encompassing methods are needed to improve evidence interpretation of modern ammunition. This research aims to fill these demands by developing standard materials and alternative methods to characterize and interpret IGSR.

This study developed primer GSR (pGSR) standards from sixty discharged primers that were fully characterized by three techniques. The number of GSR particles, …

Wild And Wonderful: How Both A Local And National Newspaper Framed West Virginia Leading Up To The 2016 Election, Emily Grace Martin

Graduate Theses, Dissertations, and Problem Reports (ETD)

During the 2016 Presidential election, journalists from all over the country flocked to West Virginia to try to understand the draw to then-candidate Donald Trump. There is a well-documented history of outsiders flooding the state and its surrounding Appalachian states to attempt to make sense of the current political situation, all while operating off of stereotypes and preconceived notions about the people of the Mountain State. This study aims to determine how stereotyping and the concept of framing or othering — when in-groups create out-groups — were used by a local West Virginia paper, as well as a national newspaper …

Does A Brief Mindfulness Induction Change Delay Discounting In Individuals With High Levels Of Obsessive-Compulsive Symptoms?, Cierra B. Edwards

Graduate Theses, Dissertations, and Problem Reports (ETD)

Obsessive-compulsive disorder (OCD) is a mental illness characterized by obsessions and compulsions that cause significant distress and/or functional impairment. Delay discounting, the decline in value of a reward with the delay of its receipt, is linked to a range of maladaptive behaviors and mental disorders. Increased delay discounting is related to obsessive-compulsive disorder (OCD), reflecting that compulsive behaviors that aim to counteract obsessive thoughts are viewed as uncontrollable by the sufferer. We aimed to experimentally reduce delay discounting in individuals with high levels of obsessive-compulsive symptoms through a brief mindfulness induction. Eligible participants were randomly assigned to receive either a …

Targeting The Cerebrovasculature In Sepsis: A Focus On The Brain Microvascular Endothelium, Divine C. Nwafor

Graduate Theses, Dissertations, and Problem Reports (ETD)

The blood-brain barrier (BBB) is a critical interface between the systemic circulation and the brain. It is a specialized multicellular unit composed of brain microvascular endothelial cells (BMECs), pericytes, a basement membrane, and astrocytic end foot processes. BMECs are a principal component of the BBB that provide the structural framework needed for the stringent transport of molecules into the brain. BMEC dysfunction permits the trafficking of neurotoxins from systemic circulation into the brain, which ultimately exacerbates BBB dysfunction and neuroinflammation. Studies have shown that BBB dysfunction is a key determinant of cognitive decline in sepsis. However, there are critical knowledge …

A Case Study Investigating Therapy Methods Used By Speech Language Pathologists Working With Children With Autism Spectrum Disorders, Julia Byers Van Volkenburg

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study was an in-depth examination of the experiences of five speech language pathologists (SLPs) working with children with Autism Spectrum Disorders (ASD) during language therapy sessions. This case study aimed to better understand the various therapy techniques being used by the SLPs through interviews and a reflective journal kept by the researcher. It also sought to examine the ways those therapy techniques may have included creative dramatics. Through thematic analysis, major and minor themes emerged from the interviews. The main theme of the study was the importance of individualizing therapy for students with ASD. Individualizing therapy was supported by …