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Graduate Theses, Dissertations, and Problem Reports (ETD)

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The Role Of The Rx Homeobox Gene In Development Of The Eye And Pituitary Gland., Elena A. Kozhemyakina Jan 2005

The Role Of The Rx Homeobox Gene In Development Of The Eye And Pituitary Gland., Elena A. Kozhemyakina

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rx is a paired-like homeodomain transcription factor that is expressed in the developing eyes, ventral forebrain, hypothalamus, posterior pituitary, and the pineal gland. In adults, Rx is primarily expressed in the neural retina, ciliary bodies, pineal gland, hypothalamus, and posterior pituitary. Rx mutations in multiple species are associated with anophthalmia. During the course if this work, we identified a human patient with anophthalmia and sclerocornea that carries mutations in the RX gene. One allele of the patient carries a Q147X mutation, which causes a truncation of the RX protein at the beginning of the homeodomain. The other allele possesses an …

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Fourth Graders' Development Of Multimedia Portfolios., Veronica D. Kuminkoski-Yurt Jan 2005

Fourth Graders' Development Of Multimedia Portfolios., Veronica D. Kuminkoski-Yurt

Graduate Theses, Dissertations, and Problem Reports (ETD)

A review of the literature indicates there is a need for alternative assessment practices for elementary students. Seven fourth grade students in a small rural school will learn how to construct an electronic portfolio that will exhibit their progress and achievement. The contents of the portfolio must include student participation in the selection as well as provide evidence of self-reflection. This study researches a computer-based portfolio, which uses HyperStudio software. This program will allow students to create sound, video, graphics, and animation during the construction process. As students build their multimedia portfolios, they will learn how to use technology as …

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Arylhydrazine Carcinogenesis And The Synthesis Of C8-Arylpurine Oligonucleotides: A Study Of Dna Adduct Effects On Dna Conformation And Stability., Jonathan R. Daft Jan 2005

Arylhydrazine Carcinogenesis And The Synthesis Of C8-Arylpurine Oligonucleotides: A Study Of Dna Adduct Effects On Dna Conformation And Stability., Jonathan R. Daft

Graduate Theses, Dissertations, and Problem Reports (ETD)

Agaricus bisporus mushrooms have been shown to be carcinogenic due to their arylhydrazine content, but the mechanism of the carcinogenesis has not been elucidated. Clues into the mechanism have been discovered in the metabolism of the arylhydrazines to arenediazonium ions and aryl radicals. Both of these compounds form DNA adducts, which may be relevant to carcinogenesis. Arenediazonium ions have been shown to activate signal transduction proteins associated with DNA damage and apoptosis. Previously, our group had shown that arenediazonium ions activate the apoptotic protein AP1. The activation of AP1 has been correlated to aryl radial generation, since the AP1 activation …

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The Addiction Severity Index As A Predictor Of Outcome For Inpatients With A Dual-Diagnosis., Larry Spencer Glenn Jan 2005

The Addiction Severity Index As A Predictor Of Outcome For Inpatients With A Dual-Diagnosis., Larry Spencer Glenn

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Addiction Severity Index is the most widely used intake instrument for substance abuse treatment. The National Comorbidity Study (Kessler et al. 1994) indicated that 13.7% of the United States population suffers from a concurrent substance use and mental health disorder within a lifetime. This study explored the utility of the Addiction Severity Index (ASI) and demographic factors as predictors of treatment as rated by staff at discharge. The records of 146 individuals with substance use and mental health diagnoses admitted to a freestanding residential treatment center in Northwestern Pennsylvania were examined to access the predictive properties of the ASI …

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Predicting Adherence In Injury Rehabilitation: Utility Of A Screening Tool And Physical Therapists' Predictions., Emma Jayne Grindley Jan 2005

Predicting Adherence In Injury Rehabilitation: Utility Of A Screening Tool And Physical Therapists' Predictions., Emma Jayne Grindley

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to explore protection motivation theory's (PMT) utility in guiding research in a general orthopedic rehabilitation facility. Further questions were whether a screening incorporating the Sports Injury Rehabilitation Beliefs Scale (SIRBS), Positive and Negative Affect Scales (PANAS), and a barriers checklist, and/or physical therapists' (PTs') perceptions were valid measures that could predict patients' adherence behavior. Adherence behavior was measured using the Sports Injury Rehabilitation Adherence Scale (SIRAS) and attendance behavior groupings which were classified as drop out/no drop out, no show/non no show, and cancellation/no cancellation. This study was conducted in a community sports medicine …

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Distributed Bragg Reflectors Based On Gallium Arsenic Nitride/Aluminum Arsenic Nitride For Long Wavelength Vcsels., Ramkumar Vinnakota Jan 2005

Distributed Bragg Reflectors Based On Gallium Arsenic Nitride/Aluminum Arsenic Nitride For Long Wavelength Vcsels., Ramkumar Vinnakota

Graduate Theses, Dissertations, and Problem Reports (ETD)

Vertical-cavity surface-emitting lasers (VCSELs) operating at long wavelength (1.3-1.55 µm) are of great interest as inexpensive, high-performance light sources for optical communication systems. Surface emission and the small dimensions of the laser cavity are advantageous for on-chip testing, packaging, effective fibre coupling and potential low-cost fabrication. Long wavelength operation is favorable in fiber optic communications because of efficient transmission of data in silica fibres at that wavelength. However, when compared to GaAs-based VCSELs operating at wavelengths below 1000nm, which have seen a tremendous development in recent years, the progress for the long-wavelength devices has been much slower. The limited refractive …

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The Train Kiln: Preparation, Construction And Firing., Scott Darwin Cornish Jan 2005

The Train Kiln: Preparation, Construction And Firing., Scott Darwin Cornish

Graduate Theses, Dissertations, and Problem Reports (ETD)

My thesis is a documentation of the building and firing of a special type of wood kiln called a train kiln, or coffin kiln. I designed the kiln using the materials that were available to me at little or no cost. My goal in this project was to design and build an efficient, user-friendly kiln, which produces unique results on fired work. Included in this thesis is documentation of the building, loading and firing of the kiln, as well as material and tool lists, kiln specifications, and a firing schedule.

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An Acting Process Analysis And Record Of The Wvu 2004 Production Of "Kolonists" With A Focus On The Character Alexei., Marc Louis Friedman Jan 2005

An Acting Process Analysis And Record Of The Wvu 2004 Production Of "Kolonists" With A Focus On The Character Alexei., Marc Louis Friedman

Graduate Theses, Dissertations, and Problem Reports (ETD)

An exploration of the acting process in the play Kolonists by Stephen Dykes, focusing on the character Alexei, with special examination of the socio-political context in post-Soviet Estonia, actor/character choices, and ensemble dynamics.

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White Washed: The Representation Of Hispanics In Television National News Coverage., Matthew P. Gallagher Jan 2005

White Washed: The Representation Of Hispanics In Television National News Coverage., Matthew P. Gallagher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study analyzed Hispanics and national television news coverage. The quantitative portion of the study examined four separate weeks of television news coverage on six networks over a one year period. From the 1,106 stories examined, Hispanics were viewed in only 18 stories. And from the results it was concluded that television news networks are “white washed,” meaning that the news is based around Caucasians.

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A Regional Scale Steady-State Groundwater Flow Model Of A Steeply-Dipping Karst Aquifer, Shenandoah Valley Of West Virginia-Virginia., Jason S. Early Jan 2005

A Regional Scale Steady-State Groundwater Flow Model Of A Steeply-Dipping Karst Aquifer, Shenandoah Valley Of West Virginia-Virginia., Jason S. Early

Graduate Theses, Dissertations, and Problem Reports (ETD)

A regional equivalent porous medium (EPM) model was developed using available hydrogeologic data for the steeply-dipping karst aquifer of the Northern Shenandoah Valley of West Virginia and Virginia. The model employed uniform recharge and variable hydraulic conductivity (K) distributed at the formation level based on published field-test estimates. Three independent procedures were used for calibration, with the most acceptable approach using calibration to heads at wells and specified fluxes for springs. Although this procedure produced a reasonable regional head distribution and acceptable calibration statistics (mean error = 0.58 m, mean absolute error = 8.61 m), the use of specified fluxes …

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A Web Based Forensic Information Management System., Sriram Govindarajulu Jan 2005

A Web Based Forensic Information Management System., Sriram Govindarajulu

Graduate Theses, Dissertations, and Problem Reports (ETD)

The focus of this work was on the design and implementation of a web based forensic information management system for the West Virginia State Police Forensics Laboratory (WVSPFL). The software tool enables law enforcement agents to submit crime information to the Forensic Laboratory via a secure Internet connection. The tool also enables each of the nine units of WVSPFL to process the information electronically. Online electronic forms were created to mirror the existing paper based forms, making the transition easier. The existing processes were standardized and streamlined, there by minimizing information inconsistency. The crime information once gathered is automatically uploaded …

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A Comparison Of Forest Soils Exhibiting Various Degrees Of Nitrogen Availability: Do Tree Species Matter And Which Nutrients Are Limiting In Nitrogen Rich Environments?, Stephen E. Gress Jan 2005

A Comparison Of Forest Soils Exhibiting Various Degrees Of Nitrogen Availability: Do Tree Species Matter And Which Nutrients Are Limiting In Nitrogen Rich Environments?, Stephen E. Gress

Graduate Theses, Dissertations, and Problem Reports (ETD)

Regions downwind of industrial and agricultural pollution receive high amounts of nitrogen (N) deposition. Some forested regions now exhibit symptoms on N saturation, which is N availability in excess of biotic demand. This phenomena and the evidence that trees alter soil in ways that may influence N saturation is the basis of my research. My thesis consists of three parts: (1) Testing the association between tree species and soil properties: Can larger-scale patterns be found under individual trees?; (2) Phosphomonesterase activity in violets (Viola rotundifolia) found in watersheds exhibiting varying levels of nitrogen availability: What lies beyond nitrogen saturation?; and …

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Trying To Get It Right: Striving To Integrate Formalized Acting Methods In The Character Development Of Theresa Bedell In Rebecca Gilman's "Boy Gets Girl"., Jennifer Moses Jan 2005

Trying To Get It Right: Striving To Integrate Formalized Acting Methods In The Character Development Of Theresa Bedell In Rebecca Gilman's "Boy Gets Girl"., Jennifer Moses

Graduate Theses, Dissertations, and Problem Reports (ETD)

In April 2004, I was given the opportunity to portray Theresa Bedell in West Virginia University’s production of Boy Gets Girl. The following document details all of the steps involved in the process of developing this character. My primary methodology was the Stanislavski method because his methods require the actor to constantly strive to find the truth when developing characters.

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Remote Sensing Of Corridor Landscapes: A Case Study Of The National Road, Wheeling, West Virginia., Brian N. Renzella Jan 2005

Remote Sensing Of Corridor Landscapes: A Case Study Of The National Road, Wheeling, West Virginia., Brian N. Renzella

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research is an investigation of how remote sensing can be used to supplement traditional methods of corridor management planning. Cultural resource inventory for corridor and interpretive management practices is an important component of applications for the designation of a selected route as Scenic Historic American Byways through the National Scenic Byways Program. A case study of a section of the National Road near Wheeling, West Virginia, showed that contemporary and historic aerial photography can facilitate the identification of possible impacts on a route’s historic integrity. A previously designated historic route, the National Road was America’s first federally funded interstate …

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An Analysis Of The Shifts In Cultural Flows Between The United States And Germany, 1890-1929., Misty D. Rodeheaver Jan 2005

An Analysis Of The Shifts In Cultural Flows Between The United States And Germany, 1890-1929., Misty D. Rodeheaver

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis examines the period from the last decade of the nineteenth century to the start of the Great Depression to explore the dynamics of popular cultural flow from the United States and Germany. World War I serves as the medium for the emergence of drastic changes in the popular cultural structures of both states. This paper underscores the influence of the war as a seminal event transforming the cultural exchange between America and Germany, as well as provides an analysis of several cultural modes of expression such as music, film, architecture, and the press. Prior to World War I, …

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The Ecological Need For Prescribed Fire In Sequoia And Kings Canyon National Park, United States Of America., Chad E. Rowan Jan 2005

The Ecological Need For Prescribed Fire In Sequoia And Kings Canyon National Park, United States Of America., Chad E. Rowan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Introdution Forests throughout the West may require prescribed fire or thinning to reduce fire hazard and restore ecological processes. Aim The purpose of this study is to identify areas outside the range of historical variability based on the Time Since Last Fire (TSLF) relative to pre-Euroamerican fire return intervals. These areas with long TSLF relative to the historic fire return interval have high fire hazard and would be given high priority for prescribed burns. Location This study was performed in topographically rugged terrain ranging in elevation from 485 to 4,392m in the Sequoia and Kings Canyon National Park, California, USA. …

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The Relationships Of Trait Learning Relevance With Teacher Communication Behaviors And Learning Outcomes., Vicki E. Bennett Jan 2005

The Relationships Of Trait Learning Relevance With Teacher Communication Behaviors And Learning Outcomes., Vicki E. Bennett

Graduate Theses, Dissertations, and Problem Reports (ETD)

This investigation explored the relationship of trait learning relevance to teacher communication behaviors and student outcomes. The operationalized student outcomes were student affect for teacher, student affect for course content, and student motivation. The operationalized teacher communication behaviors were teacher clarity, teacher immediacy, and teacher socio-communicative style (assertiveness and responsiveness). Learning relevance was operationalized through use of questions based on Herzberg’s Motivator-Hygiene Theory, five educational relevance questions scaled using the generalized belief scale, and a modified organizational orientation scale. Previous research has yielded inconsistent results in relation to the nature of students’ perceptions of state learning relevance and learning outcomes. …

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"Old England In Perfection": Historical Arguments In The Jewish Naturalization Controversy Of 1753., Philip J. Hnatkovich Jan 2005

"Old England In Perfection": Historical Arguments In The Jewish Naturalization Controversy Of 1753., Philip J. Hnatkovich

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study explores the role of historical arguments in the pamphlets of the Jewish Naturalization Controversy of 1753. Arguments made from the basis of medieval English history were among the most common in the controversy and they are key to explaining not only the origins and outcome of the Jew Bill episode, but also the underlying religious and political assumptions of the eighteenth-century English political nation. An examination of these pamphlets suggests that the conflict was due to latent antisemitism and a distinctive form of political identity, both of which were inextricably connected with Constitutionalist traditions of history and law. …

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"Oleanna" And The Road To Wisdom., Andrew H. Lyons Jan 2005

"Oleanna" And The Road To Wisdom., Andrew H. Lyons

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oleanna, originally written and produced in 1992, was presented by WVU’s Division of Theatre and Dance in September 2004. While I initially perceived the challenges of playing John as rather straightforward, I soon learned that there is a great deal more to Mamet’s character than I gave him credit for. Oleanna proves an excellent example of Mamet’s use of elliptical dialogue, especially in the telephone calls, and although I initially approached this as a purely technical challenge I was delighted to find very real creative challenges lurking within. The same is true of the role that status plays in the …

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Assessment Of Constructibility And Serviceability For Straight And Skewed Steel I-Girder Bridges., Andrew Hayes Jan 2005

Assessment Of Constructibility And Serviceability For Straight And Skewed Steel I-Girder Bridges., Andrew Hayes

Graduate Theses, Dissertations, and Problem Reports (ETD)

Recent changes to the AASHTO specifications for steel bridge design have incorporated specific provisions for handling lateral flange bending in I-shaped steel girders. The primary reason for the inclusion of this effect is to allow for a unification of design provisions for both straight and curved girders. In tangent bridges and in bridges with a small degree of skew the influence of lateral flange bending in the fully composite condition in positive bending are negligible. This is predominantly due to restraint offered by the deck and the diaphragm action offered by the deck in lateral load events. However, during construction …

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Management Of The Suckled Post-Partum Beef Cows: Pasture-Based Weaning System., John F. Odhiambo Jan 2005

Management Of The Suckled Post-Partum Beef Cows: Pasture-Based Weaning System., John F. Odhiambo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Low grain prices and recurrent periods of drought have elicited interest in early weaning. Calves can efficiently utilize feed for weight gain when it is fed directly, rather than through their dams. This study evaluated calf response in three weaning systems (experiment 1) and profiles of body energy reserves in early-weaned beef cows (experiment 2). Treatment x year, and calf sex x year interaction (P < 0.05) were observed for calf weight gain. These interactions underscored the need for constant readjustments in management systems that incorporate early weaning in their programs. In experiment 2, body weight and condition score of cows were affected marginally (P = 0.06) by early weaning, whereas ribfat and rumpfat differed (P < 0.05) between earlyweaned and control cows. These findings indicated that subjective methods (body weight and condition score) should be used in combination with more objective measurements (ribfat and/or rumpfat) for accurate evaluation of energy status of beef cows. Cows of various ages responded differently to early weaning. Three to five-year-old cows exhibited greater response to early weaning as opposed to 2-year-olds and cows > 5 years of age, indicating that profit potential of the cow-calf operation can be improved by altered management of middle-aged cows.

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The Impact Of Error Propagation On Software Reliability Analysis Of Component-Based Systems., Petar Popic Jan 2005

The Impact Of Error Propagation On Software Reliability Analysis Of Component-Based Systems., Petar Popic

Graduate Theses, Dissertations, and Problem Reports (ETD)

Component based development is gaining popularity in the software engineering community. The reliability of components affects the reliability of the system. Different models and theories have been developed to estimate system reliability given the information about system architecture and the quality of the components. Almost always in these models, one of the key attributes of component-based systems, the error propagation between the components, is overlooked or not taken into account in reliability calculation. This thesis extends previous work on the Bayesian approach to reliability estimation and assessment of component-based systems by introducing the Error Propagation Probability into the model. The …

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The Effects Of Roads And Culverts On Stream And Stream-Side Salamander Communities In Eastern West Virginia., Ryan Lee Ward Jan 2005

The Effects Of Roads And Culverts On Stream And Stream-Side Salamander Communities In Eastern West Virginia., Ryan Lee Ward

Graduate Theses, Dissertations, and Problem Reports (ETD)

Roads and culverts have many effects on stream salamanders. We examined 120 culvert crossings in the Shavers Fork and Dry Fork watersheds in eastern West Virginia. Complete barriers to salamander passage occurred at 55.0% of the sites visited and partial barriers at 34.2%. Analyses showed that 20.6% of the total stream length in the Dry Fork watershed and 18.4% in the Shavers Fork watershed were isolated by barriers. We conducted salamander sampling at 16 streams and used Akaikeís Information Criterion to select the best a priori models explaining salamander diversity, richness, and abundance. Roads benefited disturbance tolerant species while negatively …

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Evaluation Of An Air-To-Ratio Derived Exhaust Flow Rate For In-Use Emissions Testing., Jason D. Bolyard Jan 2005

Evaluation Of An Air-To-Ratio Derived Exhaust Flow Rate For In-Use Emissions Testing., Jason D. Bolyard

Graduate Theses, Dissertations, and Problem Reports (ETD)

A study was conducted to evaluate the accuracy of an air-to-fuel ratio derived exhaust flow rate for emissions testing. The Mobile Emissions Measurement System (MEMS) was developed by West Virginia University to perform in-use emissions testing for several heavy-duty diesel engine manufactures. The MEMS was modified to complete this study. A review of literature describing different on-board systems and flow measurement techniques was performed to aid in the completion of this project. Most of the exhaust flow measurement techniques require the modification of the test vehicle’s exhaust system. The air-to-fuel ratio derived exhaust flow rate only requires a probe to …

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A Study Of The Effect Of An Integrated Continuum Of Intensive Crisis Intervention Services (Icicis), Including Assertive Community Treatment (Act), On Civil Commitments In North Central West Virginia., Robert D. Williams Jan 2005

A Study Of The Effect Of An Integrated Continuum Of Intensive Crisis Intervention Services (Icicis), Including Assertive Community Treatment (Act), On Civil Commitments In North Central West Virginia., Robert D. Williams

Graduate Theses, Dissertations, and Problem Reports (ETD)

The mental health service delivery system in West Virginia is ineffective at resolving psychiatric emergencies, as evidenced by a high civil commitment rate, leading to an annual {dollar}4 million non-budgeted expense to the state. West Virginia's two State hospitals are regularly at capacity, causing civil committed patients to be diverted to community psychiatric units, often at the state's expense. At one of the state's 13 Comprehensive Community Mental Health Centers (CCMHC), United Summit Center, two evidence based treatment modalities, ICICIS and ACT, are examined for their effectiveness at lowering the civil commitment rate per 100,000 and civil commitment hospitalization days. …

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The Startup And Evolution Of The State Journal: A Statewide Newspaper Reports On Business, Phillip Daniel Page Jan 2005

The Startup And Evolution Of The State Journal: A Statewide Newspaper Reports On Business, Phillip Daniel Page

Graduate Theses, Dissertations, and Problem Reports (ETD)

Two Charleston, West Virginia, brothers, Robert C. and Henry E. Payne, III, and Charleston lawyer Fred F. Holroyd established The State Journal in October 1984 as a weekly newspaper to serve a statewide business readership. The newspaper evolved through difficult early years and eventually became profitable through the sales leadership of Lorenelle White, who would purchase the newspaper with her husband in 1997. She eventually would re-establish the newspaper’s weekly publishing cycle. The Whites would sell The State Journal in 2001 to a newly formed media business, West Virginia Media Holdings, which would increase in the newspaper’s editorial resources. Since …

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Agenda Setting And Denial In The States. Nonprofit Hospital Tax Exemptions: A North Carolina Case Study., Linda Struyk Millsaps Jan 2005

Agenda Setting And Denial In The States. Nonprofit Hospital Tax Exemptions: A North Carolina Case Study., Linda Struyk Millsaps

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research has two objectives. First, it examines the nature of state-level agenda setting, comparing it to the prevailing federal level theories of agenda setting and agenda denial. Specifically it examines state tax exemptions for nonprofit hospitals in North Carolina to determine if this policy arena follows acknowledged theories of agenda setting and denial, or if there is something unique about tax policy that creates a different agenda setting dynamic. Areas of focus included the status of nonprofit hospital property tax exemptions on the policy agenda and how the issue came to that position. Interviews with legislators, lobbyists, hospital and …

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Arithmetic And Algebra Word Problems: Preservice Teachers' Content Knowledge, Attitudes, And Appreciation Of Students' Strategies, Marc D. Michael Jan 2005

Arithmetic And Algebra Word Problems: Preservice Teachers' Content Knowledge, Attitudes, And Appreciation Of Students' Strategies, Marc D. Michael

Graduate Theses, Dissertations, and Problem Reports (ETD)

The arithmetic and algebraic word problem-solving skills, strategy preferences, and attitudes of preservice elementary and secondary teachers were investigated using a mixed methodology. Teachers' appreciations of student solutions were also examined. Prospective elementary teachers demonstrated flexible problem-solving behaviors while their secondary counterparts tended to prefer algebraic strategies exclusively. In general, the prospective teachers did not demonstrate a greater appreciation for those types of strategies that were typical to their level of training. When communicating the characteristics of an effective strategy, preservice teachers were concerned with organization and clarity rather than the mathematical relevance or appropriateness of the strategy itself. The …

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Circuit Analysis Tools For Evaluating Separation Efficiency Of Dense Medium Separators, Hongyan Sun Jan 2005

Circuit Analysis Tools For Evaluating Separation Efficiency Of Dense Medium Separators, Hongyan Sun

Graduate Theses, Dissertations, and Problem Reports (ETD)

In order to evaluate the relative separation efficiency among various circuits, a modified circuit analysis tools has been developed and applied to some circuits based upon dense medium separation. The results indicated that this circuit analysis method could predict separation efficiency of different circuit configuration without using washability data.;Based on the selective function for one stage separation, the selective functions for multi-stage circuit configurations were derived, and used to simulate the various circuit configurations. The simulation results demonstrated that some circuit configurations can improve the separation efficiency.;In-plant data for two-stage dense medium cylindrical and conical cyclone circuits without recirculation were …

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Drosophila Melanogaster Protein Kinase Ck2 Interacts With And Phosphorylates The Neurogenic Repressor M8 Resulting In The Production Of A Novel Eye Phenotype, Regina L. Trott Jan 2005

Drosophila Melanogaster Protein Kinase Ck2 Interacts With And Phosphorylates The Neurogenic Repressor M8 Resulting In The Production Of A Novel Eye Phenotype, Regina L. Trott

Graduate Theses, Dissertations, and Problem Reports (ETD)

Protein kinase CK2 is a serine/threonine protein kinase ubiquitously expressed in eukaryotic organisms. The enzyme is composed of 2 alpha (catalytic) subunits and 2 beta (regulatory) subunits. The minimum consensus site for CK2 phosphorylation is S/TXXD/E, where "X" is any non-basic amino acid. Substrates so far identified include proteins associated with cell cycle regulation and development.;In Drosophila melanogaster, the Notch pathway activates members of the Enhancer-of-split Complex [E(spl)-C]. m8, which is a member of the E(spl)-C, was found to interact with and be phosphorylated by CK2. The physiological affects of CK2 phosphorylation were studied in vivo through the Gal4-UAS system. …

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