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Graduate Theses, Dissertations, and Problem Reports (ETD)

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Post-Transcriptional Regulation Of Rpos And Hema In Salmonella, Amy Madeline Jones Dec 2009

Post-Transcriptional Regulation Of Rpos And Hema In Salmonella, Amy Madeline Jones

Graduate Theses, Dissertations, and Problem Reports (ETD)

The first part of this thesis is dedicated to translational regulation of rpoS mRNA by the small noncoding RNAs (sRNAs), DsrA and RprA, in two closely related enteric bacteria, Escherichia coli, and Salmonella enterica serovar Typhimurium. The rpoS gene encodes a second vegetative sigma factor for RNA polymerase, which directs the cell's transcriptional response to general stress and entry into stationary phase. The rpoS gene is highly conserved among the gamma-branch of proteobacteria, and sRNAs are highly conserved in related species. In fact, sequence conservation is thought to have predictive value in sRNA discovery and functional conservation is largely assumed. …

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Examination Of The Use Of Assessment By Accredited Business Schools, Tracie M. Dodson Dec 2009

Examination Of The Use Of Assessment By Accredited Business Schools, Tracie M. Dodson

Graduate Theses, Dissertations, and Problem Reports (ETD)

Assessment and accreditation are common topics in the literature of higher education. These two practices can impact an institution's ability to produce high quality students, graduates, and general success as an institution. Outcomes assessment is a method of measuring student learning as a result of the educational process. Accreditation seeks to provide external validation of the quality of a program and ensure that the public and other external constituents are aware of, and can depend on, a certain standard quality level.;In academic business units, there are three accreditation agencies. These organizations include the Association to Advance Collegiate Schools of Business …

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Weak Floor Stability In The Illinois Basin Underground Coal Mines, Murali M. Gadde Dec 2009

Weak Floor Stability In The Illinois Basin Underground Coal Mines, Murali M. Gadde

Graduate Theses, Dissertations, and Problem Reports (ETD)

The research described in this dissertation is a direct consequence of the author's quest to find solutions to several problems he faced while designing optimum pillar layouts for some Illinois Basin coal mines over weak immediate floor strata. When using the existing methods of floor stability analysis, conclusions were sometimes drawn that were either not supported by real-world mining experience or in some cases even physically impossible results were obtained. Therefore, it was necessary to delve deeper into the existing methods and understand their limitations to arrive at alternatives that were consistent with practical observations. In improving upon the existing …

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Improvement Of The Mine Fire Simulation Program Mfire, Lihong Zhou Dec 2009

Improvement Of The Mine Fire Simulation Program Mfire, Lihong Zhou

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fire represents one of the most severe hazards to underground mines. A good understanding of the interaction between a mine fire and the mine ventilation network would be very crucial for fire emergency planning and hazard control. MFIRE, an underground mine fire simulation program developed in 1980's, is a tool that can be used to simulate the impacts of a mine fire event to a mine ventilation network. However, the lack of the abilities to simulate some of the important mine fire phenomena realistically hindered its wide applications. This research has been carried out to improve the MFIRE program. The …

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Development Of A Coal Reserve Gis Model And Estimation Of The Recoverability And Extraction Costs, Chandrakanth Reddy Apala Dec 2009

Development Of A Coal Reserve Gis Model And Estimation Of The Recoverability And Extraction Costs, Chandrakanth Reddy Apala

Graduate Theses, Dissertations, and Problem Reports (ETD)

The United States has the world largest coal resource and coal will serve as the major and dependable energy source in the coming 200 years or more. However, the amount of recoverable coal reserve depends on the geological formations and quality of the coal seams, future development of mining technology and the extraction cost. A NETL sponsored cooperative research was conducted by the researchers at Carnegie Mallon University (CMU) and West Virginia University (WVU) to estimate the recoverable coal reserve. This thesis presents some of the works that WVU performed to enhance the coal reserve model including characterization of coal …

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Interleukin (Il)-1 Regulates Ozone-Induced Nerve Growth Factor (Ngf) And Substance P (Sp) Release In Bronchoalveolar Lavage Fluid (Balf) In Mice, Joshua S. Barker Dec 2009

Interleukin (Il)-1 Regulates Ozone-Induced Nerve Growth Factor (Ngf) And Substance P (Sp) Release In Bronchoalveolar Lavage Fluid (Balf) In Mice, Joshua S. Barker

Graduate Theses, Dissertations, and Problem Reports (ETD)

Previous studies show that both IL-1 and NGF increase synthesis of SP in airway neurons after either direct application or ozone exposure. We hypothesize that NGF mediates ozone-induced IL-1 effects on SP, similar to the pathway described for sympathetic neurons in culture. The current study specifically focuses on the effect of ozone on IL-1, NGF, and SP levels in mice BALF and if these mediators can be linked in an inflammatory-neuronal cascade in vivo. The experiments demonstrate in vivo ozone exposure induces an increase of all three proteins in mouse BALF and that both ozone-induced increases in NGF and SP …

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A Software Response To Hard Drive Encryption, Justin John Mccarty Iii Dec 2009

A Software Response To Hard Drive Encryption, Justin John Mccarty Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Current computer forensic practices primarily advise analysts to review and search all digital evidence within designated labs. This practice does not take into account the possibility of encryption software being present on the suspect machine. As a result, possible evidence may become unrecoverable to the investigator. This paper will review the current standard operating procedures used by law enforcement, complications with current procedures with regard to encryption, and the design of a free to use utility for law enforcement to facilitate live analysis.

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In Situ Structural And Compositional Analysis Using Rheed Electrons Induced X-Rays, Sandeep Chandril Dec 2009

In Situ Structural And Compositional Analysis Using Rheed Electrons Induced X-Rays, Sandeep Chandril

Graduate Theses, Dissertations, and Problem Reports (ETD)

The use of x-ray fluorescence (XRF) for in-situ chemical analysis of a sample surface using an electron gun for reflection high energy electron diffraction (RHEED) analysis has been studied by many groups and the technique is usually referred to as RHEED-total-refection-angle x-ray spectroscopy (RHEED-TRAXS), as the angle for x-ray detection was set close to the angle of total internal reflection.;In the current work, RHEED-TRAXS was developed as a thin film structural characterization technique based on the principles similar to the analysis of a multilayered structure by angular-dependent total-reflection x-rays. Initial experiments utilizing the technique for relative compositional analysis have also …

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Factors Associated With Treatment Acceptance And Compliance Among Incarcerated Male Sex Offenders, Carl B. Clegg Dec 2009

Factors Associated With Treatment Acceptance And Compliance Among Incarcerated Male Sex Offenders, Carl B. Clegg

Graduate Theses, Dissertations, and Problem Reports (ETD)

The present study examined factors associated with acceptance and completion of treatment among incarcerated male sex offenders. The files of 156 sex offenders who had been offered treatment at a medium-security state prison were reviewed. Participants were divided into three groups: those who refused treatment from the outset (n = 59); those who began treatment, but later dropped-out or were expelled due to non-compliance ( n = 61); and those who completed treatment or were in an advanced stage of treatment and had never been non-compliant (n = 36). Data were collected for the following variables: age, education, race, marital …

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Incremental Development And Cost-Based Evaluation Of Software Fault Prediction Models, Yue Jiang Dec 2009

Incremental Development And Cost-Based Evaluation Of Software Fault Prediction Models, Yue Jiang

Graduate Theses, Dissertations, and Problem Reports (ETD)

It is difficult to build high quality software with limited quality assurance budgets. Software fault prediction models can be used to learn fault predictors from software metrics. Fault prediction prior to software release can guide Verification and Validation (V&V) activity and allocate scarce resources to modules which are predicted to be fault-prone.;One of the most important goals of fault prediction is to detect fault prone modules as early as possible in the software development life cycle. Design and code metrics have been successfully used for predicting fault-prone modules. In this dissertation, we introduce fault prediction from software requirements. Furthermore, we …

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Mechanism By Which Dietary Polyunsaturated Fat Regulates Lipogenic Gene Expression, Alison Bloom Kohan Dec 2009

Mechanism By Which Dietary Polyunsaturated Fat Regulates Lipogenic Gene Expression, Alison Bloom Kohan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Primary hepatocytes treated with non-esterified polyunsaturated fatty acids have been used as a model for describing the inhibitory effects of dietary polyunsaturated fats on lipogenic gene expression. This model resembles starvation or uncontrolled diabetes but not dietary fat ingestion, which is delivered to liver via chylomicron remnants. Chylomicron remnants enriched with polyunsaturated fatty acids stimulate a unique pattern of intracellular signaling. These remnants enhance p38 mitogen activated protein kinase (MAPK) activity but do not inhibit insulin signaling or induce extracellular-signal related kinase (ERK) or AMP-activated protein kinase (AMPK) activation as do non-esterified polyunsaturated fatty acids. Polyunsaturated fatty acids-enriched remnants and …

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Cryptic Subtelomeric Rearrangements And Studies Of Telomere Length, Jasen Lee Wise Dec 2009

Cryptic Subtelomeric Rearrangements And Studies Of Telomere Length, Jasen Lee Wise

Graduate Theses, Dissertations, and Problem Reports (ETD)

Study One. An estimated five percent of individuals with unexplained mental retardation (MR) have chromosomally unbalanced subtelomere regions. Around half of these individuals inherited the imbalance from a parent with a balanced rearrangement. The frequency of carriers for cryptic balanced translocations is unknown. To determine this frequency, blood samples received from 565 out of 978 phenotypically normal, unrelated individuals were examined using fluorescent in situ hybridization (FISH) probes to analyze all subtelomere regions. No balanced subtelomeric rearrangements were identified. The frequency of balanced cryptic translocations in the general population was estimated to be 1/8,000 from the literature. However, three females …

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The Role Of Urban Forests In Sustainable Tourism Development: A Case Study Of Savannah, Ga, Stacy L. Casinelli Dec 2009

The Role Of Urban Forests In Sustainable Tourism Development: A Case Study Of Savannah, Ga, Stacy L. Casinelli

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this study was to identify a range of stakeholder (tourism providers, resource managers, elected officials, tour operators and tourists) perceptions, issues and concerns of Savannah, Georgia's, urban forests to help develop opportunities for the growing sustainable tourism industry. Stakeholder interviews were conducted using an open ended, semi-structured questionnaire. Based on the three dimensions and the scope of sustainable development, the themes, trends and patterns that emerged were utilized to assess the current issues facing Savannah's urban forest. Through content data analysis, four overarching themes surfaced: development, preservation, balance, and diversity. The results of this study indicate a …

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Characteristics Of Subordinate Follicles Following Removal Of The Dominant Follicle: Induction Of Selection, Matthew Dean Dec 2009

Characteristics Of Subordinate Follicles Following Removal Of The Dominant Follicle: Induction Of Selection, Matthew Dean

Graduate Theses, Dissertations, and Problem Reports (ETD)

In mammals, ovarian follicles begin as primordial follicles. Over time these follicles are gradually activated and begin growth. As they proceed, granulosal and thecal cells are acquired. Eventually, the follicles become dependent on FSH for survival. Two or three times each cycle, a transient increase in FSH allows a cohort of follicles, including the follicle that will ovulate, to continue growing. However, these follicles secrete inhibin and estradiol, which suppress FSH secretion. Eventually, all the follicles of the cohort undergo atresia due to the declining concentrations of FSH, except for the follicle which has the potential to ovulate. This follicle …

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A Process Examination Of The Framing Effect In Younger And Older Adult Medical Decision Making, Erin L. Woodhead Dec 2009

A Process Examination Of The Framing Effect In Younger And Older Adult Medical Decision Making, Erin L. Woodhead

Graduate Theses, Dissertations, and Problem Reports (ETD)

Older and younger adults are susceptible to a decisional bias when faced with medical decisions, which results in different treatment decisions when presented with survival or mortality data. Although this bias, termed the framing effect, has been demonstrated in multiple studies, no published studies have attempted to determine the decisional process older and younger adults engage in when presented with different information frames. The current study used a think-aloud procedure to examine decisional process differences in younger and older adults who did and did not demonstrate a framing effect. All participants were presented with two data formats (interval and cumulative …

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Optimization Of A Retrofit Urea-Scr System, Clinton R. Bedick Dec 2009

Optimization Of A Retrofit Urea-Scr System, Clinton R. Bedick

Graduate Theses, Dissertations, and Problem Reports (ETD)

Oxides of nitrogen (NOx) emissions from legacy diesel engines are often many times over currently mandated standards, contributing significantly to degradation of air quality and negative environmental impact. A retrofit urea-SCR (selective catalytic reduction) aftertreatment system offers a viable solution for reducing NOx emissions from older diesel engines. A stand-alone retrofit urea-SCR aftertreatment system was previously developed by West Virignia University (WVU) engineers, implementing a pre-SCR NOx sensor, open-loop feed-forward control, and stoichiometric NOx reduction logic. During experimental testing at WVU, the urea-SCR system demonstrated NOx reductions of 2% to 53%, depending on the test cycle. In order to optimize …

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Superintendent Efficacy Investigation Of A Six -Component Leadership Framework For Developing Effective Learning Environments, Frank D. Devono Dec 2009

Superintendent Efficacy Investigation Of A Six -Component Leadership Framework For Developing Effective Learning Environments, Frank D. Devono

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study, using the Superintendent Efficacy Questionnaire (Devono, Diaz & Callejo Perez, 2008), compared 413 West Virginian K--12 superintendents', principals' and teachers' perceptions of 6 superintendent leadership components: staff development administration, communication of mission, curriculum/instruction expertise, collaboration with the school board, consensus-building ability, and political bearing.;A Kruskal-Wallis test produced significant results for 2 components: the superintendent's ability to (a) clearly articulate a mission that establishes direction/priorities for the system (chi2 = 10.99; df = 2; p = 0.004), and (b) work with the local school within the budget (chi2 = 7.260; df = 2; p = 0.027). Related sample t …

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Capturing The Essence And The Coalescence Of Presence Within The Community Of Inquiry Of An Online Graduate Course In Multicultural Diversity, Katherina C. Muller Dec 2009

Capturing The Essence And The Coalescence Of Presence Within The Community Of Inquiry Of An Online Graduate Course In Multicultural Diversity, Katherina C. Muller

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study, students who are preservice and inservice teachers participated in an asynchronous online graduate course in multicultural diversity during the fall of 2008. Their emails, threaded discussions, cross-cultural community interactions, and final reflections were examined and analyzed revealing their perceptions of their experiences in this course. The main relevance of the study was to provide insight and new understandings of learning online.;The Community of Inquiry provided the framework within which these insights and understandings were drawn. Four archived course components were analyzed: emails, threaded discussions, cross-cultural community interactions, and final reflections. A coalescence of presence was found throughout. …

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Abet Accreditation Criteria, Outcome H And Global Competencies In Engineering Education, Elisabeth Sanchez-Goni Dec 2009

Abet Accreditation Criteria, Outcome H And Global Competencies In Engineering Education, Elisabeth Sanchez-Goni

Graduate Theses, Dissertations, and Problem Reports (ETD)

The dissertation focuses on one aspect of the accreditation process of engineering programs in the United States, which is conducted under the standards of the Accreditation Board for Engineering and Technology (ABET). Engineering programs seeking accreditation are required to comply with the so called Engineering Criteria 2000 (EC2000), which has been divided into eleven "learning outcomes," labeled a through k. The dissertation addresses one of them, "Outcome h", which specifically calls for "the broad education necessary to understand the impact of engineering solutions in a global, economic, environmental, and societal context.";The dissertation examines what engineering departments, from the Southern Regional …

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Stock Returns, Risk Factor Loadings, And Model Predictions: A Test Of The Capm And The Fama -French 3 -Factor Model, Daniel Suh Dec 2009

Stock Returns, Risk Factor Loadings, And Model Predictions: A Test Of The Capm And The Fama -French 3 -Factor Model, Daniel Suh

Graduate Theses, Dissertations, and Problem Reports (ETD)

This paper presents the results of time-series tests of the Capital Asset Pricing Model (CAPM) and the Fama-French 3-factor (FF3) model in the estimation of equity capital from a perspective of corporate investment decision-making. This paper addresses Fama-French (1992, "The Cross-section of Expected Stock Returns," Journal of Finance) who find that the CAPM beta has little explanatory power in cross-sectional tests of the Fama-French portfolios sorted on market capitalization and book-to-market ratios.;The main tests of this paper are (1) the equivalence of the predicted stock risk premia of the CAPM and the FF3 model, or how significantly the two model-predicted …

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Implementing Energy Release Rate Calculations Into The Lamodel Program, Morgan M. Sears Dec 2009

Implementing Energy Release Rate Calculations Into The Lamodel Program, Morgan M. Sears

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mining activity at increasingly greater depths and the tragedy at Crandall Canyon that claimed the lives of nine miners have forced coal bump research to the forefront of mining engineering research. Near the end of the last century, the National Institute for Occupational Safety and Health (NIOSH) and the former U.S. Bureau of Mines investigated the mechanics, conditions, and mitigation of coal bumps. Unfortunately, the exact mechanics of coal bumps is still not fully understood. Following this period, research into coal bumps in the United States has been limited.;The Energy Release Rate (ERR) calculation quantifies the dissipation ("release") of the …

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Adsorptive Removal Of Nitrogen From Coal-Based Needle Coke Feedstocks Using Activated Carbon, Sreeja Madala Dec 2009

Adsorptive Removal Of Nitrogen From Coal-Based Needle Coke Feedstocks Using Activated Carbon, Sreeja Madala

Graduate Theses, Dissertations, and Problem Reports (ETD)

A low percentage of nitrogen in needle coke feedstocks is desired for the reduction of puffing during the process of graphitization of needle coke. The purpose of the present study is to investigate the removal of nitrogen species from a coal-based needle coke feedstock, when treated with both commercial activated carbon, Nuchar SA 20 and "coal-derived" activated carbon, WVUAC 900-15. Koppers coal tar distillate (CTD), which has 1.1% wt starting nitrogen content, is selected as the needle coke feedstock. A series of experiments was performed to establish a standard procedure for the removal of nitrogen species. Using the established experimental …

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Design Of A Test Stand For Alternate Fuel And Ignition Systems Testing, Patrick Wildfire Dec 2009

Design Of A Test Stand For Alternate Fuel And Ignition Systems Testing, Patrick Wildfire

Graduate Theses, Dissertations, and Problem Reports (ETD)

There is a growing interest in improving engine versatility through the capacity to run on more than one fuel. To aid in this effort, ongoing research is being conducted to investigate a novel system using microwave plasma ignition designed with the goal of allowing spark ignited engines to run on multiple fuels. To accomplish the testing of this system, it was necessary to design and fabricate a test stand including an engine and instrumentation capable of measuring the performance of the ignition system within the environment of a running engine, and capable of testing this system on multiple fuels. It …

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A Reconceptualized Analysis Of The West Virginia 4-H Global Education Program: Attitudes Of Agents, Specialists, And Volunteers, Ahmed M. Abdulai Dec 2009

A Reconceptualized Analysis Of The West Virginia 4-H Global Education Program: Attitudes Of Agents, Specialists, And Volunteers, Ahmed M. Abdulai

Graduate Theses, Dissertations, and Problem Reports (ETD)

Global Education has been and continues to be an important aspect of American higher educational curriculum. Its importance is evidenced not only at the formal educational sector, but also within youth development programs like the West Virginia 4-H program.;The author contends that, the importance of global education is more relevant in the 21st century due to the increasing interdependence of the global world. The study looks at the perceived attitudes of 4-H Agents, Specialists, and Volunteers towards global education and how such an educational program affects the youth development of the 4-Her.;The present study, which is partly based on John …

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Initiating A Practice Change: Prescribing Probiotics Concurrently With Antibiotic Therapy, Patty Hermosilla Dec 2009

Initiating A Practice Change: Prescribing Probiotics Concurrently With Antibiotic Therapy, Patty Hermosilla

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background and significance. Over half of all hospitalized patients are treated with antibiotics and antibiotic use is rising. There has been a 500% increase in antibiotic associated diarrhea, a common side effect of antibiotic use, in the last decade. Probiotics are a safe and cost effective measure to prevent or reduce antibiotic associated diarrhea.;Problem statement. The majority of patients admitted to the medical intensive care unit receive antibiotic therapy; however, few are prescribed probiotic therapy concurrent with antibiotics.;Project design. Education was provided to health care providers regarding antibiotic associated diarrhea and the benefits of probiotic use. A guideline was developed …

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The Evaluation Of A Nutrition Education And Fitness Program With A Contest Component Among College Students Using The Re-Aim Framework, Michelle L. Bartlett Dec 2009

The Evaluation Of A Nutrition Education And Fitness Program With A Contest Component Among College Students Using The Re-Aim Framework, Michelle L. Bartlett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Only recently have practitioners and researchers targeted the population of college students in studying interventions aimed at increasing health behaviors. There is evidence that a proportion of college students are making attempts on their own to remedy weight gain and poor physical fitness with little or no guidance, which is reflected in consistently rising obesity rates in this population. Thus, the need for organized and effective interventions is illustrated. The impact of an intervention can be determined through evaluation research. Although the RE-AIM model has not yet been used to evaluate single-site, university-sponsored, college-student weight-loss/fitness programs, it provided a useful …

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Population Ecology, Residents' Attitudes, Hunter Success, Economic Impact, Modeling Management Options And Retention Time Of Telazol Of West Virginia Black Bears, Christopher W. Ryan Dec 2009

Population Ecology, Residents' Attitudes, Hunter Success, Economic Impact, Modeling Management Options And Retention Time Of Telazol Of West Virginia Black Bears, Christopher W. Ryan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The West Virginia Black Bear Research Project (WVBBRP) was initiated in 1972 to investigate population parameters, growth rates, home ranges, and habitat uses of a declining American black bear (Ursus americanus ) population. The West Virginia Division of Natural Resources (WVDNR) continued to monitor the black bear population and increased monitoring efforts and sample sizes in the 1990's as the black bear population increased in size and expanded its range. As part of the WVBBRP, we investigated black bear population ecology. Our objectives were to estimate reproductive rates, estimate survival and cause-specific mortality rates, examine effects of special hunting seasons …

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The Fearful Face And Beyond: Fmri Studies Of The Human Amygdala, Jillian E. Hardee Dec 2009

The Fearful Face And Beyond: Fmri Studies Of The Human Amygdala, Jillian E. Hardee

Graduate Theses, Dissertations, and Problem Reports (ETD)

The amygdala has been labeled as a "detector of threat", evidenced by its heightened response to fearful faces in human neuroimaging studies. A critical element of the fearful face is an increase in eye white area, hypothesized to be crucial for the rapid detection of fear in another's face. Yet other facial expressions can also increase eye white area in a manner that is similar (a lateral shift in gaze) or identical (surprise) to fear. It is unknown if the amygdala can differentiate between these types of increases in eye white area and those that are specifically associated with fear …

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Soldiers And Stereotypes: Mountaineers, Cultural Identity, And World War Ii, C. Belmont Keeney Dec 2009

Soldiers And Stereotypes: Mountaineers, Cultural Identity, And World War Ii, C. Belmont Keeney

Graduate Theses, Dissertations, and Problem Reports (ETD)

To what extent are Appalachian stereotypes true and how much is pure fabrication? This study seeks to answer this question by examining the experiences of West Virginia soldiers during World War II. Appalachian hillbillies, believed to be culturally backward, uncivilized, isolated, and prone to violence, were often sent straight to the infantry because it was believed that their wild mountain heritage made them inherently better fighters. Using interviews, letters, and a collection of over 1,200 firsthand written accounts of Appalachian veterans collected by West Virginia University in 1946, this study traces the evolution of the cultural and individual identities of …

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Properties Of A Dehydroalanine Analog Of Glutathione: A Reactive Electrophilic Busulfan Metabolite, Cody J. Peer Dec 2009

Properties Of A Dehydroalanine Analog Of Glutathione: A Reactive Electrophilic Busulfan Metabolite, Cody J. Peer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Gamma-Glutamyldehydroalanylglycine (EdAG) is a dethiolated, electrophilic metabolite of glutathione (GSH) derived from the Phase II conjugation of GSH with busulfan catalyzed by glutathione S-transferase (GST). It was demonstrated in this dissertation that EdAG could be successfully synthesized and shown to be a metabolite of busulfan from in vitro metabolism by enzymes in human liver cytosol. The electrophilic EdAG was reactive toward the cellular thiols cysteine and glutathione (GSH), and toward cysteine residues in reduced bovine insulin B-chain and bovine serum albumin in vitro , as well. EdAG was demonstrated to be a reversible noncompetitive inhibitor of GSTs, specifically GSTA1-1 at …

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