Digital Commons Network^™

Regulator Of G Protein Signaling-12 (Rgs12) In Dopaminergic And Kappa Opioid Receptor-Dependent Signaling And Behavior, Joshua David Gross

Graduate Theses, Dissertations, and Problem Reports (ETD)

Dopaminergic neurotransmission is critically involved in the etiology and treatment of many psychiatric and neurological disorders. One modulator of dopaminergic neurotransmission is the kappa opioid receptor (KOR) -- a G protein-coupled receptor (GPCR) that is densely expressed within dopaminergic neurons and circuits. GPCRs are tightly regulated by a variety of intracellular signaling molecules, including Regulator of G Protein Signaling (RGS) proteins. Canonically, RGS proteins act as GTPase accelerating proteins (GAPs) on GTP-bound Ga subunits following GPCR activation, thereby hastening the rate at which GPCR-mediated G protein signaling is terminated. However, some RGS proteins exhibit more complex mechanisms of action on …

Investigation Of Bovine Conceptus Galectins And The Effect Of Bovine Conceptus Secretory Proteins On The Endometrial Epithelial Transcriptome, Heather Lynn Baldwin

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pregnancy loss in cattle is greatest in the first 30 days of gestation. Near d15 of gestation, bovine conceptuses elongate and express LGALS1, an immunosuppressive glycan-binding protein believed to be essential for implantation. However, other members of the bovine galectin family, including LGALS3, LGALS4, LGALS7, LGALS8, LGALS9 and a galectin-13-like gene (LGALS13L), also may be expressed by the elongating conceptus. Therefore, galectin expression in elongating, d16 bovine conceptuses and intercaruncular endometrium as well as elongating, d14 ovine conceptuses and intercaruncular endometrium was assessed by RT-qPCR. Angus heifer and Texel ewes underwent estrous cycle synchronization and either inseminated or not bred. …

Copper-Catalyzed Regioselective Boracarboxylation Of Vinyl Arenes: Catalytic Efficiency And Synthetic Utility, Trina M. Perrone

Graduate Theses, Dissertations, and Problem Reports (ETD)

Hetero(element) carboxylation is an appealing transformation that involves the installation of CO2 and another hetero(element) in one step. This transition metal-catalyzed one-pot synthesis provides a route to achieve highly functionalized carboxylic acid products with an abundant and cheap C1 feedstock. This difunctionalization also avoids the use of reactive metal hydride species, which is a prominent limitation of hydrocarboxylation chemistry. A copper-catalyzed regioselective boracarboxylation of vinyl arenes has been developed to access pharmaceutically relevant β-boryl-α-aryl propionic acid products. One drawback of this reaction is the necessity for high catalyst loading to achieve catalytic turnover. To circumvent this issue, the reduction of …

Evaluating Post-Fire Plantation Restoration In A Mixed Conifer Forest In The Sierra Nevada, Iris C. Allen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Forests in the western United States have experienced a shift from historical disturbance regimes in the past century. Many of these changes were induced by European settlers logging the forests and suppressing fires. In the past, the dry mixed conifer forests of California’s Sierra Nevada mountains experience frequent, low to mixed severity fires. This fire regime helped maintain a heterogeneous landscape comprised of groups of trees and openings. However, due to fire suppression and high grading logging, forest structure has changed; there are less openings and more small, fire-intolerant trees that can carry a fire into the forests crown. The …

Untangling The Reciprocal Relation Of The Behavioral And Physiological Immune Systems, Baris Sevi

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the past decade, there has been an exponential increase in the study of the Behavioral Immune System (BIS), a suite of psychological processes that serves an infectious disease avoidance function. The BIS is proposed to be inherently intertwined with the physiological immune system. However, very few studies have actually demonstrated this fundamental, theoretical assumption. The purpose of this thesis was to test the possible relation between the physiological and behavioral immune systems in two studies. In Study 1 (N = 388), the relation between implied activation of the physiological immune system and BIS sensitivity was examined. Participants who reported …

Behavioral And Anti-Inflammatory Effects Of Magl Inhibition In A Mouse Model Of Inflammatory Arthritis, Floyd Franklin Steele Iii

Graduate Theses, Dissertations, and Problem Reports (ETD)

Cannabinoids have analgesic and anti-inflammatory properties. For example, in preclinical models of acute inflammatory pain, cannabinoid receptor subtype 2 (i.e., CB_2, Table 1) agonists decrease paw inflammation and resulting pain. Yet, the anti-inflammatory properties of cannabinoid receptor manipulation in chronic inflammatory pain states are unknown. Monoacylglycerol lipase (MAGL) is the primary catabolic enzyme of the endocannabinoid (i.e., endogenous cannabinoid ligand) 2-arachidonoyl glycerol (i.e., 2-AG). Compounds that inhibit MAGL indirectly increase tissue levels of 2-AG by preventing its catabolism. MAGL inhibitors demonstrate efficacy in acute models of inflammatory/rheumatic disease. Thus, in this study, the MAGL inhibitor JZL184 was tested …

Distribution And Habitat Use Of Benthic Fishes In The Lower Kanawha River, West Virginia, Nathaniel V. Owens

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Kanawha River in West Virginia is a historically diverse system in terms of ichthyofauna although, like many other large rivers, it has been subjected to a suite of anthropomorphic perturbations. These include this system being modified to allow the passage of commercial vessels for industry thus altering flow regimes, increased sedimentation, water depth, and channelization, and decreased interactions with its floodplain. Additionally, the fishes of this system have suffered from deleterious historical water quality issues caused by intensive timber operations, mining, and industrial waste. Post Clean Air Act, Clean Water Act, and Surface Mining Control and Reclamation Act water …

Genetic And Pharmacologic Studies Towards Prevention Of Opioid Use Disorder, Shane W. Kaski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Endogenous opioids mediate both analgesic and affective responses to stress. While the mu opioid receptor (MOR) produces the reinforcing euphoric effects responsible for the high abuse potential of traditional opioid analgesics, the kappa opioid receptor (KOR) is hypothesized to mediate the dysphoric component of stress. With increasing rates of opioid addiction, extensive research efforts are focused on better understanding each of these systems and how they interact, with the goal of developing less addictive pain management strategies and better approaches to addiction treatment. With this in mind, we conducted both clinical and pre-clinical studies aimed at developing better treatment strategies …

Lignocellulosic Biomass Derived Activated Carbon For Energy Storage And Adsorption, Changle Jiang

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lignocellulosic biomass has been converted to hierarchical porous carbon materials which possess macro-, meso- and micro-pores. The natural structure of porous lignocellulosic structure was preserved during activation with further developed porosity by the activation. The activated carbon can be well applied to electrochemical double layer capacitor for transportation storage of ions as well as adsorbent materials for metal ion removal from wastewater.

The first chapter of this dissertation presents an introduction of biomass derived carbon and its applications. In the second chapter, both direct and indirect activation methods using carbon dioxide were adopted in this study. The results show that …

Comparing The Effects Of Swimming Exercise And Dissolved Oxygen On Important Performance Parameters Of Early-Rearing Atlantic Salmon Salmo Salar And Rainbow Trout Oncorhynchus Mykiss., Thomas B. Waldrop

Graduate Theses, Dissertations, and Problem Reports (ETD)

Swimming exercise (bodylenghts/sec), and dissolved oxygen (DO), are important environmental variables in aquaculture. While there is an obvious physiological association between these two parameters, their interaction has not been adequately studied in Atlantic salmon, Salmo salar and Rainbow trout, Oncorhynchus mykiss. Since both variables can be easily manipulated in modern aquaculture systems, we sought to assess the impact of these parameters, alone and in combination, on the performance, health, and welfare parameters of the two species. Atlantic salmon and Rainbow trout were stocked into twelve circular 0.5 m³ tanks and exposed to either high (1.5-2 BL/s) or low …

Pedagogical Thoughts On Album Des Six: A Piano Set By Les Six To Represent French Nationalism, Dipendra Sunam

Graduate Theses, Dissertations, and Problem Reports (ETD)

Les Six, formally established in 1920, included a group of young French composers: Georges Auric (1899-1983), Louis Durey (1888-1979), Arthur Honegger (1892-1955), Darius Milhaud (1892-1974), Francis Poulenc (1899-1963) and Germaine Tailleferre (1892-1983). The primary motivation for their formation was the reaction against foreign influences, especially German Romantic music, in French music. Jean Cocteau, inspired by Erik Satie’s style, proposed a new French musical aesthetic: simplicity, directness, clarity, and terseness. Les Six followed these ideals in their formative years. Their artistic association was short-lived, but while it lasted it produced Album des Six, their sole joint production, a published …

Dp Internal Codeswitching By Spanish And English Bilinguals In The Us, Yurena Castaño-Nuñez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Codeswitching studies the boundaries that exist at all linguistic levels when a speaker switches between two or more languages in a single moment of speech.

(i) En el restaurante he pedido la big hamburguesa.

‘At the restaurant I have ordered the big hamburger.’

(ii) Juan conoce la highway para llegar a Washington D.C.

‘Juan knows the highway to get to Washington D.C.’

(iii) A Carmen no le gustan los pantalones cortos. A ella le gustan los long ones.

‘Carmen doesn’t like short pants. She likes the long ones.’

This thesis concentrates on the study of switches between adjectives and Noun …

Characterization Of Lipids And The Protein Co-Products From Various Food Sources Using A One-Step Organic Solvent Extraction Process, Alleda Rose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Traditional lipid extraction methods are labor intensive and time-consuming so interest in one-step methods using various organic solvents are gaining in popularity. Extraction efficiency depends heavily on the solvents used yet comparisons between different solvents are lacking. Defatting techniques are commonly used to concentrate crude protein and may be useful for enhancing protein content in food sources. The objective of this research was to 1) determine the lipid extraction efficiencies of hexane (H), 3:2 hexane-isopropanol (HI), chloroform (C), 2:1 chloroform-methanol (CM), and methyl-tert-butyl-ether (MTBE) using a one-step organic solvent lipid extraction method on whole insect, egg yolk, and krill powders …

Induction Of Cerebral Hyperexcitability By Peripheral Viral Challenge: Role Of Cxcl10 Chemokine, Tiffany J. Petrisko

Graduate Theses, Dissertations, and Problem Reports (ETD)

Peripheral viral infections are po­tent comorbid factors that exacerbate neuro­degene­ration. Although the under­lying mecha­nisms have not been defined, neuronal hyperexcitability has been established as an underlying feature. Our lab has developed a preclinical model in which a viral mimetic, poly­inosinic-poly­cytidylic acid (PIC) is injected in­tra­peritoneally to induce an anti-viral acute phase response (APR). APR in turn elicits robust neuronal hyperexcitability. The present study was undertaken to characterize molecular mechanisms that mediate the development of hyperexcitability in response to PIC challenge. The analysis of brain tissue after PIC challenge revealed a robust elevation of CXCL10 chemokine, indicating its putative role in …

Systematic Control Of Aged Skeletal Muscle Following High-Intensity Stretch-Shortening Contraction Exercise Training: Epigenomic Regulation And Signaling Factors Underpinning Adaptation, Marshall Alan Naimo Jr.

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sarcopenia, the age-related decline in skeletal muscle mass, results in a loss of strength and functional capacity, which subsequently increases the risk of disease, disability, frailty, and all-cause mortality. Exercise is known to be an efficacious paradigm for improving health and attenuating or preventing many chronic diseases. For the previous two decades, our laboratory has established an in vivo rodent dynamometer model to explore the effects of various skeletal muscle training paradigms following stretch-shortening contractions (SSCs).The responses tohigh-intensity resistance-type exercise training (RTET) using this physiological model ranges from adaptation, characterized by enhanced skeletal muscle performance along with increased muscle mass, …

Glial Cell Expansion And Intercellular Signaling In The Developing Medial Nucleus Of The Trapezoid Body (Mntb), Ashley N. Brandebura

Graduate Theses, Dissertations, and Problem Reports (ETD)

Neural circuit formation is a complex process involving coordinated communication between neurons, glia and vascular-associated cells (VACs). Each cell type is responsible for a unique transcriptional and translational contribution to tissue maturation. Deciphering the intercellular signaling patterns which guide neural circuit formation during normal development is thus an essential step in understanding which components of neural circuit formation go awry in neurodevelopmental disorders. The medial nucleus of the trapezoid body (MNTB), located in the auditory brainstem, was used as a model system to study the dynamics of neural circuit formation because it contains a mostly homogeneous population of postsynaptic neurons …

An Assessment Of Emotional-Force And Cultural Sensitivity The Usage Of English Swearwords By L1 German Speakers, Sarah Dawn Cooper

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the usage of English swearwords by L1 German speakers and poses the following three questions: (1) Which English swearwords do native-speakers of German use? (2) In which situations do German speakers use English swearwords? Why do they use English swearwords instead of their German counterparts? (3) What emotional weight do they apply to swearwords in English (i.e. How taboo are they)? In an effort to understand Germans’ use of swearwords today, potential attributing factors were assessed and evaluated in a 15-question survey comprised of both qualitative and quantitative components that was taken by 403 participants from Germany …

Johannes Brahms’S Fünf Ophelia-Lieder Performance History, Cultural Context, And Character Study As It Pertains To Johannes Brahms’S Fünf Ophelia-Lieder: A Performer’S Perspective, Caryn Alexis Crozier

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document aims to contribute research on the lesser known and under researched vocal works by Johannes Brahms, particularly his Fünf Ophelia Lieder. This song cycle, while not written to be performed on its own, originally, is a rich technical resource for beginning singers and advanced singers alike. Brahms wrote a simple melody with sparse accompaniment that really allows the performer to focus on language, technique, or dramatic interpretation. This document looks at Brahms’s setting of Ophelia’s mad scene through many different lenses including theoretical, by analyzing each song musically and dramatically, historical analysis of Hamlet and its many iterations, …

Using The Enrichment Triad Model To Explore Place And Support Curriculum In One Rural Gifted Program, Myriah Mae Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Using narrative inquiry, this case study explored how one teacher, who had deep connections to place, learned and utilized the place-based Enrichment Triad in her rural Appalachian elementary gifted program. Data collected included teacher, student, and parent interviews and classroom observations. Data were analyzed using a socio-cultural narrative analysis and narrative thematic analysis. Results from analysis created thick descriptions of the teacher’s journey to teaching, her existing practices, her experiences learning and implementing the framework, and her future curricular intentions. Developments in the teacher’s practice included establishment of scope and sequence, a sharing of power, and embracing students’ identities through …

Improving Broiler Performance Utilizing Modern Feed Additives, Niles Rourke Ridgeway

Graduate Theses, Dissertations, and Problem Reports (ETD)

Modern broiler production strives to make modest improvements regarding broiler performance. This remains a goal as global population increases thusly increasing the quantity of an affordable, high quality source of protein. In recent years, an added stressor to achieving prior goals is mostly related to consciousness of environmental and consumer health. For decades, integrators have utilized minimal amounts of antibiotics as a barrier to most pathogens affecting the microbiome of a broiler’s gastrointestinal tract. Regulatory efforts have now prohibited most of previous sub-therapeutic medicine and started an in-depth scope of broiler gut health and interaction with antibiotic alternatives. In an …

Canonical Wnt Mechanisms In Neural Crest Induction, Mark Stephen Perfetto

Graduate Theses, Dissertations, and Problem Reports (ETD)

Canonical Wnt signaling is a pathway that is critical for normal development and the progression of disease. The canonical Wnt signaling pathway was put together carefully by synthesizing decades of research. Over those decades, canonical Wnt signaling was found to be crucial for nearly all aspects of development, but of importance to this thesis, is a key regulatory factor for the development of the highly migratory multipotent stem cells, the neural crest. As our knowledge of the importance of canonical Wnt signaling grew, research is being conducted to further our understanding of how canonical Wnt signaling communicates with other signaling …

Metal Nanomaterials: Immune Effects And Implications Of Physicochemical Properties On Sensitization, Elicitation, And Augmentation Of Allergic Disease, Katherine Adair Roach

Graduate Theses, Dissertations, and Problem Reports (ETD)

Many allergenic metals are being manufactured in nanoparticulate forms. Although the decreased size profile of metal nanomaterials has been consistently associated with more pronounced lung toxicity compared to larger materials, it is unclear if metal-induced immunotoxic effects exhibit a similar size-dependency.

The central hypothesis of these studies was that metal nanoparticles cause more pronounced immunomodulatory effects on allergic processes than larger forms of respective metals. Moreover, it was anticipated that, similar to their inflammatory potential in airways, the magnitude of these responses would correlate best with the dose metric of surface area.

The first set of studies utilized fine and …

Artificial Immune System For Unmanned Aerial Vehicle Abnormal Condition Detection And Identification, Ryan G. Mclaughlin

Graduate Theses, Dissertations, and Problem Reports (ETD)

A detection and identification scheme for abnormal conditions was developed for an unmanned aerial vehicle (UAV) based on the artificial immune system (AIS) paradigm. This technique involves establishing a body of data to represent normal conditions referred to as “self” and differentiating these conditions from abnormal conditions, referred to as “non-self”. Data collected from simulation of the UAV attempting to autonomously fly a pre-decided trajectory were used to develop and test a scheme that was able to detect and identify aircraft sensor and actuator faults. These faults included aerodynamic control surface locks and damages and angular rate sensor biases. The …

The Marvel Sonic Narrative: A Study Of The Film Music In Marvel's The Avengers, Avengers: Infinity War, And Avengers: Endgame, Anthony Walker

Graduate Theses, Dissertations, and Problem Reports (ETD)

This document explores the music of three films in the Marvel Avengers enterprise: Marvel’s The Avengers, Avengers: Infinity War, and Avengers: Endgame. It does not examine the second film of the series, Avengers: Age of Ultron. The document seeks to uncover and further understand the music’s function in each film and to determine its continuity throughout all three films. This study uses a combination of film music studies and theory-based analysis to support the findings with the aim of identifying connecting musical elements that stretch throughout these three films. This includes the use of leitmotif/themes, previously …

Critical Physicochemical Properties For Nanoparticle Toxicity: Impact Of Surface Coating And Size On Particle-Induced Cell Transformation And Inflammatory Response, Tiffany Kornberg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Nanoparticles, which measure 100 nm in at least one dimension, have surged in development, production, and use for a wide range of applications. However, the rapid pace of development for these emerging materials with unclear/unknown toxicity profiles makes it difficult to adequately assess health risk associated with exposure. One critical obstacle which limits scientific research to fill these critical knowledge gaps is the lack of accurate and predictive models for nanotoxicology studies, particularly those which involve occupationally relevant exposure scenarios (pulmonary exposure to low dose of particles in the circulating air). Typically, animal models are used to assess potential systemic …

The Acute Effect Of Propanil On Reproductive Hormones, Ovarian Gene Expression And Splenic Cells In C57bl/6 Female Mice, Malia Danielle Berg

Graduate Theses, Dissertations, and Problem Reports (ETD)

Earlier evidence indicates exposure to propanil, a post-emergent herbicide used on rice and wheat fields, increases the number of antibody-secreting cells (ASC) in the spleen of mice after immunization with heat-killed Streptococcus pneumoniae (HKSP, 2x10⁸ CFU). Interestingly, an intact hypothalamic-pituitary-gonadal (HPG) axis is required for this immune response assessed on day 7-10 after immunization in female mice. Hence, an overarching premise is that propanil arbitrates the connection between the reproductive and immune system by altering the concentration of hormones involved in the HPG axis, which may then affect the populations of splenic cells. Hypotheses were tested with C57Bl/6 female …

Evaluating The Consistency Of A Breathing Flow Recording Device Across Different Elastomeric Half-Mask Respirators, Samuel Jennings Munnis

Graduate Theses, Dissertations, and Problem Reports (ETD)

The National Institute of Occupational Safety and Health (NIOSH) is the organization in charge of approving respiratory devices in the United States, as permitted by 42 CFR Part 84. Standards that NIOSH uses to certify powered air-purifying respirators (PAPRs) are based on respiratory data collected before the 21^st century. With new technologies, it is possible to gain a more accurate understanding of breathing flow of healthcare workers (HCWs). A new portable breathing flow recording device developed by The S.E.A. Group was intended for use with a Sundström SR100 mask with a P100 filter. This study tests the device with …

Thermal Performance Of Growth At Consumption Maximum (C-Max) And Routine Metabolic Rate (Rmr) In Brook Trout Salvelinus Fontinalis From Four Populations In Central Appalachia, Cory Michael Hartman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Growth of an individual is the end result of many physiological processes stemming from the consumption of food items. These processes can be influenced by many variables including temperature. Current bioenergetics models for Brook Trout Salvelinus fontinalis suggest growth increases with temperature until an inflection point of 20.2°C. With a warming climate, and water temperatures expected to rise, the ability of a population to adapt to the warming environment is crucial to the survival of the species. For this study we sought to see how spatial variation would influence the specific consumption, conversion efficiency, and specific growth at consumption maximum …

Historic Log Buildings As Archives Of Past Forest Ecology, Kristen K. De Graauw

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation is composed of three separate but related manuscripts with the common theme of using historic log buildings from the central Appalachian Mountain region of eastern North America as ecological archives. In Chapter 1, I explore the biases, limitations, and ecological applications of tree-ring data from historic log buildings. European immigrants selected trees from a forested stand based on species, log sizes, and construction locations. Despite this selection bias, ecological information can be gleaned from historic log buildings, which offer a complementary record of past forest ecology and represent a site type that is not often associated with old-growth …

Promoting Cognitive And Fine Motor Development In The Art Room For Young Students With Exposure To Trauma: An Exploratory Case Study., Tiffany Marie Minuci

Graduate Theses, Dissertations, and Problem Reports (ETD)

Despite the overwhelming research that suggests visual artmaking experiences can produce positive outcomes for individuals, many state art education policies fail to implement and integrate the arts into core academic curriculums. Specifically in the state of West Virginia, kindergarten through twelfth grade students often lack the access to a quality and consistent art education. With high teacher-pupil ratios and inclusive learning environments, educators face the difficult task of meeting all the individual needs of their students’ in the classroom. This not only affects whole student populations, but also poses risks for children who have been exposed to trauma. Traumatic experiences …