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Utilizing The Parts Of Common Food Products As Functional Ingredients Part 1: Separation And Concentration Of Ω-3 Pufa-Rich Phospholipids By Hydration Of Krill Oil Part 2: Functional Properties Of A Concentrated Egg Yolk Protein Powder Using A One-Step Organic Solvent Extraction Process, Casey Lynn Showman

Graduate Theses, Dissertations, and Problem Reports (ETD)

The food industry separates food products into different parts to better utilize the functional and nutritional benefits of each fraction. The overall focus of this thesis is on separating and utilizing fractions and co-products from food manufacturing as functional ingredients. Therefore, the objective of this research was to 1) evaluate the effectiveness of water degumming to separate omega-3 PUFA-rich phospholipids from other lipids found in krill oil and 2) determine how different organic solvents would affect physiochemical and functional properties of egg yolk protein recovered from spray-dried egg yolk using a novel one-step organic solvent extraction method.

The aim of …

Stream Salamander And Benthic Macroinvertebrate Community Responses To Imidacloprid Exposure, Sara M. Crayton

Graduate Theses, Dissertations, and Problem Reports (ETD)

The insecticide imidacloprid is widely used to mitigate hemlock (Tsuga spp.) mortality resulting from the invasive hemlock woolly adelgid (HWA), but evidence suggests that

imidacloprid can have negative effects on adjacent stream systems. Imidacloprid exposure causes physiological changes, bioaccumulation, and reduced survival in anurans, and induces physiological impairment and reduced survival in benthic macroinvertebrates. In this thesis, we assessed whether HWA treatments using imidacloprid affect stream salamander abundances and benthic macroinvertebrate community composition. We also investigated whether imidacloprid or its metabolites bioaccumulate in benthic macroinvertebrates or salamanders and cause sublethal effects. We conducted this study in two National Park …

Soil Health Assessment In Reclaimed Mine Soils, Katie Danielle Stutler

Graduate Theses, Dissertations, and Problem Reports (ETD)

West Virginia is one of the top coal-producing states in the nation, which has resulted in over 500,000 acres of highly disturbed, anthropogenic mine soils caused by surface mining. Mine soils are often rocky and contain low organic matter content, low plant-available nutrients, and toxic metals, which could result in poor soil health and low productivity. Soil health has been defined as the continued capacity of soil to function as a living ecosystem that sustains plants, animals, and humans. It is assumed that with time since reclamation, mine soil properties and related functions may change, and soil health will improve …

Development Of Improved Acellular Pertussis Vaccines Through Inclusion Of The Rtx Antigen Or Induction Of Mucosal Immunity, Dylan Tyler Boehm

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pertussis is a human respiratory disease, primarily caused by the Gram-negative pathogen Bordetella pertussis. The infection is most severe and can be life-threatening in young children and infants where it manifests as a series of paroxysmal coughs. The disease is more commonly known as whooping cough, due to the whoop omitted during a massive inspiratory effort to bring air back into the lungs. Pertussis is a toxin-mediated disease that persists due to an early release of toxins that allow that bacteria to evade the cells of the innate immune response. The inhibition of the host response continues as toxin activity …

Influence Of Climate Change And Prescribed Fire On Habitat Suitability And Abundance Of The High-Elevation Endemic Cow Knob Salamander (Plethodon Punctatus), Carl David Jacobsen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Amphibians are facing global declines due to climate change, loss and degradation of habitat, invasive species, and disease. The Appalachian region of the eastern USA is a global biodiversity hotspot for salamanders, which are considered keystone species that influence nutrient dynamics in terrestrial and aquatic food webs. There are high rates of salamander endemism in the Appalachian region, with many species restricted to isolated, high elevation areas. The Cow Knob Salamander (Plethodon punctatus) is one such species. It is only found at elevations >675 m (most populations are above 900 m) on Shenandoah Mountain, North Mountain, and Nathaniel …

Role Of Sox9 In Cancer Stem Cell Regulation In Lung Cancer Chemoresistance And Particle-Induced Carcinogenesis, Maria Voronkova

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lung cancer is a leading cause of cancer-related death worldwide. Every year it claims more lives than other common malignancies, breast, prostate, and colorectal cancers, combined. And despite recent improvements in disease diagnostics and treatment, drug resistance and tumor relapse remain major contributors to low patient survival. Both issues have been largely attributed to the existence of cancer stem-like cells (CSCs) or tumor initiating cells (TICs). CSCs are a small cell population within heterogeneous tumors that is inherently resistant to apoptosis, chemo- and radiation therapy. Thus, it is critical to elucidate CSC-specific signaling pathways and identify early biomarkers for therapeutic …

Modeling And Development Of The Dynamic Environmental Simulation Chamber (Desc) For Calibration Of Air Quality Monitoring Sensors, Filiz Kazan

Graduate Theses, Dissertations, and Problem Reports (ETD)

The thesis describes the development steps for the dynamic environmental simulation chamber (DESC) and carbon dioxide sensor calibration by using DESC. The sensors are Figaro, COZIR and K30 CO2 sensors

Reconciling The Dissonance Between Historic Preservation And Virtual Reality Through A Place-Based Virtual Heritage System., Danny J. Bonenberger

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study explores a problematic disconnect associated with virtual heritage and the immersive 3D computer modeling of cultural heritage. The products of virtual heritage often fail to adhere to long-standing principles and recent international conventions associated with historic preservation, heritage recording, designation, and interpretation. By drawing upon the geographic concepts of space, landscape, and place, along with advances in Geographic Information Systems, first-person serious games, and head-mounted Virtual Reality platforms this study envisions, designs, implements, and evaluates a virtual heritage system that seeks to reconcile the dissonance between Virtual Reality and historic preservation. Finally, the dissertation examines the contributions and …

The Effect Of Bovine Galectin-1, A Conceptus Secretory Protein, On The Endometrial Transcriptome, Lindsay Faye Grose

Graduate Theses, Dissertations, and Problem Reports (ETD)

Early embryonic loss in cattle is detrimental to reproduction thus, the profitability of both the beef and dairy industries. Therefore, an important aspect of study is to find techniques that beef or dairy producers could use to decrease early embryonic loss Galectin-1, the protein that was investigated in this research is important in modulating the maternal immune system in rodent and human early pregnancies by promoting fetomaternal immune tolerance. The role of galectin-1 within the reproductive tissues has not been thoroughly investigated in cattle. Galectin-1 is located in the caruncular and intercaruncular regions of the bovine endometrium, specifically the maternal …

A Study Of Gastrointestinal Pathophysiology, Emily Minor

Graduate Theses, Dissertations, and Problem Reports (ETD)

Though the duodenum is the primary site of iron absorption, the colon also contains iron transporters and participates in iron absorption. The purpose of this study was to characterize colonic iron absorption in control patients and those with ulcerative colitis (UC), a disease that frequently results in iron deficiency. Using RT-qPCR and western blot analysis, we found that colonic expression of the apical iron importer, divalent metal transporter 1 (DMT1), is equal to duodenal expression. However, colonic expression of ferroportin 1 (FPN1), an iron exporter, is only about one-quarter that of the duodenum. Patients with active ulcerative colitis were found …

Avian Occupancy Dynamics And Canada Warbler (Cardellina Canadensis) Environmental Correlates On A Central Appalachian Elevation Gradient In West Virginia, Gordon W. Dimmig

Graduate Theses, Dissertations, and Problem Reports (ETD)

Mountains are important areas for avian diversity and conservation since they have steep elevational and environmental gradients. In the central Appalachian Mountains, several songbird species have trailing edge populations that are restricted to high elevations. Climate change and other factors can cause species distributions to change, which is of particular concern for birds in restricted trailing edge populations. Additionally, the Canada Warbler (Cardellina canadensis) breeds throughout the Appalachian Mountains and is species of conservation concern due to range-wide population declines. For this project, I used avian point count data from the central Appalachian Mountains to: (1) evaluate the …

Discrimination And Behavioral Responses To Communication Signals Compared Across Apteronotids., Danielle Leigh Dillon-Seeger

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory systems are often uniquely tailored to encode behaviorally relevant signals and comparative studies across species can thus reveal how evolutionary changes shape sensory functions. The structure of communication signals varies widely between ghost knifefish species. Recent findings suggest that the nervous system co-adapted to the various signal structures observed across species to support different sensory behaviors. The aim for this thesis was to compare the sensory behavior of 3 species of ghost knifefish to contrast their behavioral performance with the known differences in neurophysiology. We hypothesize that for the different signal types and species, the ability to discriminate small …

Browsing Via Sonification, Taylor C. Cutlip

Graduate Theses, Dissertations, and Problem Reports (ETD)

Based on unexpected results in her previous research, Dr. Frances Van Scoy became inspired to develop a tool that allows the user to navigate spaces using auditory instead of visual cues to detect objects or anomalies in a given space in an effort to overcome the encountered obstacles. This problem report details the work of one of her grad students in exploring different open source software for developing the tool as well as research into gestures and other considerations when furthering this research into the third dimension at a future date.

Fundamental Mechanism Of Time Dependent Failure In Shale, Neel Gupta

Graduate Theses, Dissertations, and Problem Reports (ETD)

In underground coal mines, mining activity disturbs the natural equilibrium state of in-situ stresses. The induced and in-situ stresses deform the rockmass surrounding the mine openings. Primary roof supports impede the deformation of the rockmass overlying the entries. However, failure can occur in the bolted rockmass, causing fatalities and injuries. The rockmass failure is erratic, and its occurrence often varies from a few days to months and years after the opening of the entry. One of the often-neglected factors in this process is the effect of time on the stability of mine openings, which can often be observed in the …

Determining The Role Of Nudt7 In The Regulation Of Cellular Coa Levels And Metabolism, Stephanie Anne Shumar

Graduate Theses, Dissertations, and Problem Reports (ETD)

Coenzyme A (CoA) is an essential cofactor required for hundreds of metabolic processes. Because it is such a critical cofactor, CoA levels are tightly regulated. In the fasted state and in diabetic mice, the concentration of CoA increases dramatically in the liver. This phenotype is associated with constitutively low CoA degradation, a process that is emerging as a potentially important mechanism for CoA regulation. Nudt7 and Nudt19 are two mammalian peroxisomal enzymes with CoA-degrading activity, which are highly expressed in the liver and kidney, respectively. Limited information is available on the biochemistry of Nudt7 and Nudt19; the structural basis for …

Evaluating The Effects Of Exogenous Enzyme Supplementation On Broiler Growth, Victoria E. Ayres

Graduate Theses, Dissertations, and Problem Reports (ETD)

Experiments were conducted to investigate the effects of exogenous enzymes in poultry diets. In Chapter 4, a study was conducted to determine the effects of a corn-expressed recombinant carbohydrase (AC1) on broiler performance and digesta viscosity in high non-starch polysaccharide (NSP) diets through 21 days of age. One day-old Hubbard × Cobb 500 chicks were assigned to 6 dietary treatments. Each treatment consisted of 12 replicate pens of 10 birds. The positive control diet (PC) was a corn and soybean meal formulation. The negative control diet (NC) included 10% wheat and 10% corn distiller’s dried grains with solubles (DDGS). The …

Technology Use By Teachers Of Deaf And Hard-Of-Hearing Students, Nichole K. Zirzow

Graduate Theses, Dissertations, and Problem Reports (ETD)

This nationwide study explored the types and frequency of technology used by

teachers of deaf and hard-of-hearing (d/hh) students to design and deliver instruction, as

well as the types and frequency of technology being used by teachers of d/hh students to

promote communication and collaboration. It also examined the extent of professional

development in the area of technology use with d/hh students, and the related challenges

experienced by these teachers. The following research questions guided this study.

Research question 1. What kinds of technology do teachers of d/hh students use

to design and deliver academic content-based learning activities? With what …

An Assessment Of The Methods Used To Teach Content Skill Sets Required For The Introduction To Agriculture, Food, And Natural Resource Course, Lee I. Wright

Graduate Theses, Dissertations, and Problem Reports (ETD)

Numerous studies have shown that students learn best when theory is applied to hands-on experiences. Traditionally, vocational agriculture, which evolved into agricultural education, has incorporated hands-on learning. For example, students typically raise livestock and crops as part of supervised agricultural experiences; learn to use chain saws, tractors, hay rakes, balers and other equipment found on farms; and basic metal working skills. They also sample soil and water to test for mineral levels and types of pollution that might affect agricultural practices. The purpose of this study, which incorporates a survey of agricultural education teachers in West Virginia, is to determine …

Characterization Of Major Flavonols In Ramps (Allium Tricoccum), Wijdan M. Dabeek

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ramps (Allium tricoccum) is a traditional plant in the eastern Appalachian Mountains. Ramps have been used in Appalachian folk medicine for its health promoting roles in lowering blood pressure and cholesterol. Information on the chemical composition of bioactive components responsible for the potential health benefits are scarce. Analysis of the chemical composition in similar Alliumplants showed that flavonols, induced similar health benefits. Therefore, the aim of this work is to characterize major flavonol conjugates in ramps. The secondary aim is to develop a preliminary quantification protocol. Flavonol glycosides were extracted in 50% methanol and 3% acetic acid. …

Ferritin: An Iron Deficiency Biomarker In Collegiate Female Distance Runners, Ty Bigelow

Graduate Theses, Dissertations, and Problem Reports (ETD)

Context: Iron deficiency is a concern in distance runners due to the implications that hemoglobin production becomes impaired limiting oxygen uptake and tissue oxidation in the body. Ferritin is a biomarker used to diagnose iron deficiency. The related signs and symptoms of iron deficiency are associated with decreased performance and prolonged recovery. Objective: The purpose of this study was to investigate whether the change in ferritin levels affect measures of performance, measures of recovery, and rate of perceived exertion in female endurance runners. Design: The study was a descriptive longitudinal study. Setting: The study was conducted at a Mid-Atlantic Division …

The Role Of Sirt1 In Skeletal Muscle Function And Repair Of Older Mice, Matthew John Myers

Graduate Theses, Dissertations, and Problem Reports (ETD)

Human skeletal muscle is a highly metabolic tissue necessary for mobility and coordination. Responsible for approximately one-fifth of the resting human metabolism, skeletal muscle is also an important regulator of metabolites like glucose and contributes to the regulation of body temperature. Although there is a gradual decline in muscle mass associated with aging, a certain percentage of the population suffer from severe muscle mass and strength deterioration, classified as sarcopenia (5-13% for people aged 60-70 years old, 11-50% for those 80 or older). Sarcopenia is linked to increased morbidity and mortality rates in the elderly population, while annual healthcare costs …

Resurgence Of Academic Responses, Catherine Louise Stephens

Graduate Theses, Dissertations, and Problem Reports (ETD)

Resurgence is the recurrence of a previously reinforced response after a more recently reinforced response is placed on extinction. Resurgence may explain the recurrence of socially appropriate behavior, including academic responding, but this had not yet been empirically demonstrated. The aim of this study was to determine if resurgence would occur when a participant solved quadratic equations using multiple methods. Each participant was taught two methods of solving quadratic equations across experimental phases, followed by a phase in which neither method resulted in the correct solution. In the first phase, only simple factoring was reinforced. In the second phase, only …

The Impact Of Post Translational Modification On The Aggregation Of The Ss-Amyloid Peptide In The Presence Or Absence Of Lipids, Albert Walton Pilkington Iv

Graduate Theses, Dissertations, and Problem Reports (ETD)

Alzheimer’s disease (AD) is a neurodegenerative condition afflicting 5.7 million Americans with no effective treatments. The pathology of AD is described by the formation of neurotoxic oligomers formed by the self-assembly of amyloid-β (Aβ). The affinity of oligomers for cellular membranes disrupts calcium homeostasis and is believed to be the underlying cause of neurotoxicity; however, due to the metastability of oligomers, it has been difficult to understand their role in membrane interactions and self-assembly. Here we utilize post translational modifications (PTM) to understand the role of specific amino acids on Aβ₄₀-membrane interactions and self-assembly. We employ ThT, AFM, …

Immunity-Based Framework For Autonomous Flight In Gps-Challenged Environment, Mohanad Al Nuaimi

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this research, the artificial immune system (AIS) paradigm is used for the development of a conceptual framework for autonomous flight when vehicle position and velocity are not available from direct sources such as the global navigation satellite systems or external landmarks and systems. The AIS is expected to provide corrections of velocity and position estimations that are only based on the outputs of onboard inertial measurement units (IMU). The AIS comprises sets of artificial memory cells that simulate the function of memory T- and B-cells in the biological immune system of vertebrates. The innate immune system uses information about …

Factors Influencing Survival, Productivity, And Population Growth Of Eastern Wild Turkeys In Northeastern South Dakota, Reina M. Tyl

Graduate Theses, Dissertations, and Problem Reports (ETD)

The population of eastern wild turkeys in northeastern South Dakota appeared to be expanding soon after reintroduction in the 1990s, however, recent harvest trends suggest declining abundance in the region. Spring turkey hunting expenditures are projected to be around $6.5 million in South Dakota, with about $3 million of those expenditures occurring within the northeastern prairie region. However, the number of birds harvested during the spring prairie firearm season has been declining since 2010. Due to concerns about a declining population, the autumn hunting season was closed in 2014. The cause of the apparent decline is unclear. Updated demographic information …

Using Lidar To Approximate Keystone Structure And Evaluate Management Practices In Potential Habitats Of The Endangered Karner Blue Butterfly (Lycaeides Melissa Samuelis), Brandyn Balch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Keystone structure is the spatial structure required by a given species, at a scale that is determined by that species’ needs and mobility. The endangered Karner blue butterfly (Lycaeides melissa samuelis, hereafter KBB) has a keystone structure that incorporates trees and bushes to provide the mixture of sun and shade required to fulfil its life functions. Airborne light detection and ranging (lidar) is a potentially invaluable tool for characterizing keystone structures. However, lidar has yet to be utilized to evaluate structural suitability of KBB habitats. Therefore, I investigated the use of lidar for characterizing critical attributes of KBB habitat structure, …

Elucidating A Common Mechanism Of Proteasome Impairment In Neurodegenerative Disease And Its Pharmacological Intervention, Tiffany Ann Thibaudeau

Graduate Theses, Dissertations, and Problem Reports (ETD)

Proteostasis is maintained by several systems in the cell including the ubiquitin-proteasome system (UPS), chaperones, chaperone-mediated autophagy, and macroautophagy. The UPS is the principle route for the degradation of intracellular misfolded, damaged, or unneeded cellular proteins and has a critical role essential every cell process, including: cell cycle progression, transcriptional regulation, genome integrity, apoptosis, immune responses, and neuronal plasticity. When the efficiency of protein degradation is perturbed, misfolded and damaged protein aggregates can accumulate to toxic levels and cause neuronal dysfunction, which may underlie many neurodegenerative diseases. It is widely appreciated that soluble oligomers of misfolded proteins (e.g. Aβ, Alzheimer’s; …

Amazon Nights Ii: Electric Boogaloo-Neural Adaptations For Communication In Three Species Of Weakly Electric Fish, Kathryne M. Allen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Sensory systems have to extract useful information from environments awash in noise and confounding input. Studying how salient signals are encoded and filtered from these natural backgrounds is a key problem in neuroscience. Communication is a particularly tractable tool for studying this problem, as it is a ubiquitous task that all organisms must accomplish, easily compared across species, and is of significant ethological relevance. In this chapter I describe the current knowledge of what is both known and still unknown about how sensory systems are adapted for the challenges of encoding conspecific signals, particularly in environments complicated by conspecific-generated noise. …

Framing Effects On Fear Of Terrorism And Willingness To Sacrifice Civil Liberties, Ellory Ruth Dabbs

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research was to determine whether differences in the way the media frames an act of violence leads to different reactions by consumers. In particular, it was hypothesized that the ideology and race of the perpetrator would lead to differences in perceptions of whether or not the attack was terrorism. A vignette-style experiment was performed using respondents recruited via MTurk. Four versions of the vignette were evenly distributed to 441 respondents, changing whether the frame contained a photo, the ideology, and the name of the perpetrator. Using measures of fear from this data it was then investigated …

Compound-Specific Isotope Analysis Of Amino Acids In Biological Tissues: Applications In Forensic Entomology, Food Authentication And Soft-Biometrics In Humans, Mayara Patricia Viana De Matos

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this work we demonstrate the power of compound-specific isotope analysis (CSIA) to analyze proteinaceous biological materials in three distinct forensic applications, including: 1) linking necrophagous blow flies in different life stages to their primary carrion diet; 2) identifying the harvesting area of oysters for food authentication purposes; and 3) the ability to predict biometric traits about humans from their hair.

In the first application, we measured the amino-acid-level fractionation that occurs at each major life stage of Calliphora vicina (Robineau-Desvoidy) (Diptera: Calliphoridae) blow flies. Adult blow flies oviposited on raw pork muscle, beef muscle, or chicken liver. Larvae, pupae …