Digital Commons Network^™

Genetics Of Mycangial Development And Host Colonization Within The Ambrosia Symbiosis, Mckeon James Laws

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ambrosia beetles, a polyphyletic group of weevils, utilize pouch-like structures at various locations on the body to transport for their sole food source, their fungal partner. Ambrosia fungi are known to originate from multiple unrelated fungal lineages with no consensus of traits required to become a fungal cultivar. While the biology and ecology of both partners is in many cases well understood, the genetic underpinnings of this fascinating mutualism remain a mystery. The aim of this dissertation is to add to the understanding of the ambrosia symbiosis by providing insights into its molecular and genetic underpinnings through RNA sequencing data, …

Walking In The Footsteps Of My Ancestors: A Deep Map Of Cherokee Forced Removal, Deborah Lyn Kirk

Graduate Theses, Dissertations, and Problem Reports (ETD)

In this study of the place of Cherokee forced removal, I use a Third Space approach to blend Indigenous Methodologies, Deep Mapping, Geospatial mapping and analysis, and 3D Geography. Using a hybrid platform that provides a mélange of media types, storytelling forms, and interactive tools, I reimagine and retell the story of the Cherokee “Trail of Tears.”

In accordance with Cherokee philosophies of duyuktv, harmony, and “a good mind” I first sought out Cherokee sponsorship from community respected and culturally knowledgeable Cherokee citizens; partnered with the National Trail of Tears Association (NTOTA); submitted my research proposal to the Cherokee …

Electronic Cigarettes Increase Blood-Brain And Blood-Tumor Barrier Drug Efflux In A Preclinical Lung Cancer Model Of Brain Metastasis, Cullen Parker Wolford

Graduate Theses, Dissertations, and Problem Reports (ETD)

This dissertation (a) provided a comprehensive review of the known therapeutic challenges in nicotine delivery system users with lung cancer and (b) investigated the impact of electronic cigarettes on blood-brain/blood-tumor barrier function and efficacy of small molecule KRAS inhibitors in a syngeneic lung cancer brain metastasis model. The blood-brain barrier is a physiochemical barrier that limits systemic delivery of therapeutics into brain. When tumors invade the central nervous system, the blood-brain barrier is disrupted and subsequently forms a blood-tumor barrier. Despite the inherent leakiness and variable permeability of the blood-tumor barrier, it continues to restrict anti-cancer drugs from accumulating at …

Evaluation Of Engineering Controls In Mitigation Of Fecal And Chloride Contamination To An Urban Stream, Gabriel J. Zenny

Graduate Theses, Dissertations, and Problem Reports (ETD)

Fecal coliform contamination is a major contributor to surface water pollution in the United States’ (U.S.) waterways, whether from combined sewer overflows (CSOs) (point source pollution), runoff from precipitation and snowmelt (nonpoint source pollution), or exfiltration from aging and failing infrastructure. In addition to fecal bacteria, chloride contamination is also a major, prevalent surface water contaminant impacting U.S. waterways. Chloride contamination is predominantly released into U.S. waterways as nonpoint source pollution, endemic to northern areas that use industrial brine and salt as a form of mitigation for ice accumulation on roadways. The purpose of this research was to investigate impairment …

Managing The Legacy Of Historical Land Use In Central Appalachia: Soil Health, Carbon Storage, And Restoration Across Disturbed Ecosystems, Kyra R. Dukich

Graduate Theses, Dissertations, and Problem Reports (ETD)

Historical mining, intensive timber harvesting, agriculture, and atmospheric acid deposition have left lasting impacts on soil health throughout the Central Appalachian region, reducing soil productivity, carbon storage, and ecosystem resilience. This thesis evaluated restoration strategies across two long-term research systems in West Virginia to determine how land-use history and soil amendments influence soil health, carbon dynamics, and ecosystem recovery. The first study examined reclaimed mine lands and marginal agricultural soils planted with switchgrass (Panicum virgatum) or willow (Salix spp.) and treated with biochar or inorganic fertilizer. Soil greenhouse gas production, aggregate stability, total soil C:N, pH, and soil organic matter …

Factors Influencing Eastern Wild Turkey (Meleagris Gallopavo Silvestris) Productivity And Nesting Chronology In West Virginia, Kirstin Palumbo

Graduate Theses, Dissertations, and Problem Reports (ETD)

Identifying drivers of variation in the timing of breeding behaviors, such as courtship activities and nesting chronology, is important for the effective management of avian species. Likewise, productivity is an important component of game bird population dynamics and can be broken up into multiple components such as the probability of nesting, clutch size, and nest success. One species that experiences a relatively long reproductive season is the Eastern wild turkey (Meleagris gallopavo silvestris). Contemporary research in the southeastern United States has raised concerns regarding declines in both wild turkey populations and overall productivity. West Virginia, however, is unique …

Does Diverse Seeding Adoption Reduce Production Costs? The Case Of Diverse Forage Seeding Strategies In West Virginia., Ivan Andres Wong

Graduate Theses, Dissertations, and Problem Reports (ETD)

Feed and harvested forage represent a major share of production costs in Appalachian cow-calf systems, where a pronounced summer forage gap forces producers to rely on purchased hay. This thesis evaluates whether adopting diverse forage seeding strategies reduces production costs relative to the existing pasture management practice, accounting for the spatial heterogeneity of West Virginia’s production environments. The analysis proceeds in two steps. First, an unsupervised K-means clustering of 14 farms enrolled in the NRCS Conservation Innovation Grant field trial groups the farms into two agroclimatic clusters, Highland-Cool and LowlandWarm, using elevation, growing-season Growing Degree Days, growing-season precipitation, and soil …

Romantic Narrative In Three Early Piano Cycles By Robert Schumann: Papillons, Op. 2; Fantasiestücke, Op. 12; And Kinderszenen, Op. 15, Lingqing Huang

Graduate Theses, Dissertations, and Problem Reports (ETD)

This research project compares Romantic instrumental narrative in Robert Schumann’s Papillons, Op. 2; Fantasiestücke, Op. 12; and Kinderszenen, Op. 15, with particular attention to how pianists communicate cyclic continuity in performance. In this study, narrative continuity refers to relationships formed across ordered short pieces through contrast, interruption, return, literary association, fantasy, memory, tonal and motivic recall, and the shaping of sound and time. Comparative score analysis shows that Schumann creates a distinct form of continuity in each cycle. In Papillons, he creates a public, ballroom-based form through masquerade, waltz-like dance, abrupt entrances, interruption, and the gradual dissolution of the social …

Exploring The Application Of Wastewater Monitoring To Determine The Prevalence And Diversity Of Influenza On The Campus Of West Virginia University, Lakyn Renee Sanders

Graduate Theses, Dissertations, and Problem Reports (ETD)

Influenza viruses (IV) affect populations globally, with the World Health Organization (WHO) estimating that IV are responsible for an estimated 3-5 million infections and 290,000 – 650,000 deaths annually^1–3. More specifically, the 2019-2020 IV season is associated with an estimated 38 million illnesses and 22,000 deaths in the United States alone⁴. IV are rapidly spread between individuals through direct contact with infected individuals, contaminated surfaces, or aerosolized virus particles². Traditional IV infection monitoring relies on clinical screening of patients presenting influenza-like symptoms, commonly through the use of multi-target panel tests such as the BioFire …

Effect Of Delay On Reinforcer Preference And Efficacy, Ciara Jasmine Rodriguez

Graduate Theses, Dissertations, and Problem Reports (ETD)

Behavior intervention plans are used to increase desired behavior and decrease problem behavior, and these plans require the use of effective reinforcers. High-quality reinforcers that are delivered immediately tend to be the most effective, but optimal reinforcement parameters are not always feasible or appropriate in applied settings. Some reinforcers may be disruptive, teachers’ competing responsibilities may introduce delays to reinforcer delivery, and reinforcers may not be immediately accessible. Stimuli may lose their reinforcing value with delay to their receipt, and this is referred to as delay discounting. Thus, there is likely a point where a less preferred but immediately delivered …

Effects Of An Early-Onset Hfd On Hepatic Functioning In A 3xtg Model Of Ad, Matthias Hirst

Graduate Theses, Dissertations, and Problem Reports (ETD)

Background: Approximately 1 billion people currently live with obesity. Driven by factors such as a high-fat diet (HFD), this pathology can have drastic effects throughout the body, with the liver in particular being a main target of its downstream consequences. Although it is capable of storing lipids, excess fat can conglomerate in the liver and contribute to various hepatic pathologies. A specific pattern of hepatic dysfunction seen in response to an HFD is increased acetylation of mitochondrial proteins. Further, expression and activity of insulin-degrading enzyme (IDE), involved in the degradation of Aβ, are altered in response to an HFD. Aβ …

The Impacts Of Experiential Learning On Moral Development, Jacob Tyler Greenfield

Graduate Theses, Dissertations, and Problem Reports (ETD)

Burnout remains a widely studied phenomenon in the healthcare literature, with much of the focus placed on reactive mitigation strategies that lack meaningful engagement with moral development. Healthcare education programs rarely incorporate formal learning experiences that promote moral development as a means of addressing burnout symptoms. This qualitative single case study examined how health professions students describe the impact of experiential learning on their moral development. Participants engaged in adapted photovoice methods, focus groups, and interviews to reflect on how serving as a Project MUSHROOM student leader influenced their morality. Four themes emerged: 1) recognizing bias and the need to …

Gcn5l1-Mediated Lysine Acetylation Regulates Mitochondrial Dysfunction In The Aged Heart, Jackson Edmond Stewart

Graduate Theses, Dissertations, and Problem Reports (ETD)

For over a century, cardiovascular disease (CVD) has been and remains the leading cause of death globally. CVD risk and severity increase significantly with advanced age, and mitochondrial dysfunction has been implicated in the pathogenesis of aging. Furthermore, mitochondrial health is crucial for cardiac function, as one-third of total cardiomyocyte volume is occupied by mitochondria in order to meet the high energy demand of contractile and relaxational function. It is thus crucial that we understand the pathological mechanisms driving mitochondrial dysfunction in aging and how this contributes to age-associated cardiovascular disease. The current manuscript aims to fill this knowledge gap …

The Effects Of Gesture And Age On Jurors’ Perceptions Of Child Witnesses, Jihan Hani Rashed

Graduate Theses, Dissertations, and Problem Reports (ETD)

Jurors’ perceptions of child witnesses can have significant influence in judicial outcomes, particularly in cases where children’s testimony is central (Goodman et al., 1997; Goodman et al., 1998; Castelli et al., 2005). The role of gesture in shaping jurors’ perceptions is underexplored. Studies highlight that gesture enhances memory recall and reduces cognitive load, making it an important component of effective communication (Goldin-Meadow et al., 2001; Hudson & Sheffield, 1998; Cook & Fenn, 2017). The present research consisted of two studies examining whether child witnesses’ use of gesture during recall of an alleged crime affected jurors’ perceptions of witness credibility and …

Pbmc Transcriptome Analysis Following Lps Stimulation Reveals Distinct Inflammatory Strategies In Feed-Efficient And Inefficient Beef Cattle, Yarahy Leal

Graduate Theses, Dissertations, and Problem Reports (ETD)

Improving feed efficiency is a primary objective in livestock production, serving as a major driver of profitability and sustainability in beef cattle operations as feed costs continue to rise. Residual feed intake (RFI) and residual average daily gain (RADG) are widely recognized indicators of feed efficiency and are commonly used as selection traits to reduce production costs and environmental impact. Previous research suggests a functional shift in transcriptional activity associated with immunity depending on efficiency phenotype, with evidence of greater immune system robustness in more efficient animals. This dissertation examines the molecular mechanisms linking feed efficiency and immune competence and …

Early Life Adversity And Reward Reactivity In Humans, Maria C. Potts

Graduate Theses, Dissertations, and Problem Reports (ETD)

Both animal and human research has identified early life adversity (ELA) as a prominent risk factor for substance use disorder (SUD). However, the underlying mechanisms linking ELA to SUD development remain unclear. Animal studies point to differences in reward responsiveness as the primary explanation; specifically, that ELA could lead to individual differences in the attribution of incentive salience to reward-predictive cues, or the “sign-tracking” behavioral phenotype. Sign-tracking has been linked to addiction-related behaviors in both animal and human studies, but no human studies have examined whether sign-tracking could partially explain the link between ELA and SUD. In fact, human studies …

The Role Of Temperature And Carbon Source In Nutrient Acquisition For Peptidoglycan Synthesis In A Gfp-Expressing Borreliella Burgdorferi, Barrett-Anne Conner Briggs

Graduate Theses, Dissertations, and Problem Reports (ETD)

Lyme disease is the most common vector-borne disease in the United States with an estimated yearly incidence rate of more than 300,000 cases. Borreliella burgdorferi, the etiological agent of Lyme disease, is transmitted by the bite of an infected Ixodes scapularis tick and can cause debilitating pathologies affecting numerous body systems. Symptoms include fever, fatigue, joint pain, and a characteristic bull’s eye rash (erythema migrans) at the site of the tick bite. The timely and appropriate use of antibiotics effectively treats early Lyme disease; however, uncomfortable side effects reduce treatment compliance. If left untreated, long-term complications can arise including facial …

Programs Of Support For Wardship Experienced Students: Exploring Program Directors' Expectations And Experiences Through A Bioecological Framework, Adalheid Faith Crum

Graduate Theses, Dissertations, and Problem Reports (ETD)

The purpose of this research is to explore the expectations and experiences of program directors of campus support programs which center wardship experienced students. This research question guided the current study: What expectations and experiences influenced program directors’ development and reform of a wardship-centered campus support program? This inquiry applies Ürie Bronfenbrenner’s Person–Process–Context–Time (PPCT) bioecological model. Semi-structured interviewing was utilized for this case study. In this research, six directors of campus support programs centering wardship experienced students were interviewed. Additionally, this work employs content analysis of twenty-eight National Research Collaborative for Foster Alumni and Higher Education (NRC-FAHE) publications, introducing fourteen …

Optimization Of Feed Manufacturing Productive Capacity, Feed Quality, And Feed Safety Utilizing A Variety Of Pellet Die Parameters, Feed Additives, Conditioning Specifications, And Simulated Feed Bin Storages, Lucas Edward Knarr

Graduate Theses, Dissertations, and Problem Reports (ETD)

In Chapter 2, an experiment was conducted to determine if the inclusion of 1.50% mixer-added water (MAW) and/or 0.25% Azomite^® (AZM) would be suitable in alleviating concerns regarding pellet production rate and pellet quality when manufacturing feed with low-moisture corn. Furthermore, the authors aimed to determine if these additives had varying effects when utilizing different pellet die thicknesses. A 2 (Azomite; 0.00% or 0.25%) x 2 (mixer-added water; 0.00% or 1.50%) x 3 (pellet die thickness; 32 mm, 38 mm, or 45 mm) factorial arrangement was utilized to create the twelve experimental treatments, each manufactured …

Orogenesis, Timothy Ryan Butcher

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis and accompanying exhibition explore the translation of panenhenic mystical experience and the collaboration with Appalachian natural spaces into abstracted landscape images. This work aims to “map” a sensation of fundamental unity and gesture toward the ineffable by utilizing found-object stamp printing, and automatic mark-making. Inspired by practical mystic traditions and contemporary landscape painting, this body of work, entitled Orogenesis, combines intricate, abstracted ink landscape drawings and photographs to create images suggestive of the mystical patterns in the process.

Designing Dracula: A Feminist Revenge Fantasy, Really: A New Take On The New Woman, Spencer T. Lawlor

Graduate Theses, Dissertations, and Problem Reports (ETD)

This thesis will document the process of designing the costumes for Dracula: A Feminist Revenge Fantasy, Really, by Kate Hamill. It will detail a close reading of the text and how it differs from its original source material, Bram Stoker’s Dracula, an examination of the research process for this design, as well as an analysis of the design process and execution of costumes for this production. It will explore the challenges I faced as the Costume Designer on this production, including but not limited to: examining the gender dynamics involved in genderbending a well-known piece of literature, working within a …

Computational Methods For Identification Of Molecular Signatures, Weijun Yi

Graduate Theses, Dissertations, and Problem Reports (ETD)

This work develops computational methods for identifying molecular signatures from high-throughput genomic data and for modeling long non-coding RNA (lncRNA) sub-cellular localization. The response of multiple myeloma to CB-6644, a selective RUVBL1/2 complex inhibitor with potential anti-tumor activity, is analyzed to identify drug-responsive pathways and molecular signatures. Conventional gene set enrichment analysis (GSEA) often excludes low-expression genes. Here, phenotype comparison is reformulated as a supervised machine learning problem: genes most informative for discrimination are first selected using a machine learning approach, and GSEA is then applied to these machine-learning derived gene sets. This framework improves detection of CB-6644-associated pathways. For …

Reducing Inorganic Phosphorus In Poultry Diets: Nutritional Constraints And Strategies For Successful Implementation, Elizabeth Ann Lynch

Graduate Theses, Dissertations, and Problem Reports (ETD)

Applied poultry nutrition and feed manufacture science can be described as a “three-legged stool” supported by pellet quality, feed hygienics, and nutrient availability, where imbalance in any one component can compromise overall bird performance. The research presented herein primarily addresses feed hygienics and nutrient availability, with pellet quality serving as an underlying theme, to better understand how processing conditions and dietary formulation influence performance and nutrient utilization.

In Chapter 2, the objective of this study was to evaluate the effects of hygienizer retention time on bacterial reduction during feed manufacture using Enterococcus faecium as a Salmonella surrogate. Pellet mill motor …

A Fine-Scale Genomic Study Of Cheat Mountain Salamanders (Plethodon Nettingi) On Managed Public Land, Amylynn Nicole Ephraim

Graduate Theses, Dissertations, and Problem Reports (ETD)

Habitat fragmentation has the potential to isolate localized populations, particularly those which are understood to have limited dispersal capabilities. This isolation can reduce genetic diversity through the effects of inbreeding depression, and small populations are more susceptible to the impacts of genetic drift. In this study, I aim to understand the level of genetic connectivity between known occupied patches of Cheat Mountain salamanders (Plethodon nettingi; CMS) on managed public land. CMS are a federally threatened, high-elevation, West Virginia endemic species associated with red spruce (Picea rubens) forests. I used double-digest restriction site associated DNA (ddRAD) sequencing …

Plant Invasion Patterns In Understory And Soil Microbial Communities Of North Central West Virginia Mixed Hardwood Forests, Isabelle Marie Uganski

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive plants alter forest ecosystems by reducing species richness, habitat heterogeneity, and nutrient cycling as they rapidly displace native plants. Invasive plant composition can influence soil health through altering soil microbial associations and communities. By studying the mechanisms in which invasives alter above- and below-ground communities, we can better understand whole-ecosystem impacts and inform management decisions. This study examined the relationships between co-occurring invasion on above and belowground communities of mixed hardwood forests of north central West Virginia. Despite distinctive associations with aboveground characteristics (native diversity, native tree regeneration, and leaf litter density) we detected limited changes to soil microbial …

Creative Energies: Unearthing The Role Of Romantic Ecocriticism, Nicole Marie Chambers-Stahl

Graduate Theses, Dissertations, and Problem Reports (ETD)

The literary field of ecocriticism has transcended the boundaries of Academia: excited to connect to their physical environments, many of these scholars are focusing on genres like place-based writing, rhetoric of energy policy, and representations of the Earth in film and media. Interdisciplinary by nature, ecocriticism has demonstrated clear potential in its ability to intersect with concentrations like the history of science, animal studies, and environmental justice.

However, as the language around global crises is heating up —“Today it is scientists, not prophets,” writes Tobias Menely in “‘The Present Obfuscation,’” “who speak the language of apocalypse” (478)—ecocritical scholarship is at …

The People Of The North: Influencer Histories And Miniaturization In Eleventh-Century Italy, Evan Anthony Simonetti

Graduate Theses, Dissertations, and Problem Reports (ETD)

The Norman invasion of Italy in the eleventh century was a process that required political support and legitimacy for its leaders, the Hauteville dynasty. The members of this dynasty either commissioned or financed three individuals as they wrote three respective histories of the Norman conquest in order to accomplish this legitimation. In these sources, each author took inspiration from the classic works of history and poetry of the Greek and Roman world and applied symbolism and direction from their understanding of medieval Christian theology. In addition, these works catalogued examples of events, people, places, animals, and behavior as exotic objects …

Behavioral Effects Of Prenatal Ecig Aerosol Exposure On Sprague-Dawley Rats, Erin Elizabeth Wylie

Graduate Theses, Dissertations, and Problem Reports (ETD)

Maternal electronic cigarette (ECIG) use during pregnancy raises concerns about potential risks to offspring, yet its behavioral effects remain unclear. The present studies were designed to investigate how prenatal exposure to ECIG aerosols, with and without nicotine, influences the behavior of Sprague-Dawley rat offspring. Dams were exposed to aerosols containing base liquid alone, aerosols containing base liquid with 18 mg/ml nicotine, or filtered air (control) throughout gestation. Following postnatal day 65, male offspring from 17 litters completed lever-press acquisition (Experiment 1), sustained-attending (Experiment 2), and nicotine self-administration (Experiment 3) tasks. One-way analyses of variance (ANOVAs) were used to compare task …

Motivation And Well-Being: A Study Of Wvu Extension Volunteers, Amber Renae Goff

Graduate Theses, Dissertations, and Problem Reports (ETD)

Volunteers are the heart of the land-grant outreach model. They bring education and resources to communities across West Virginia. Volunteers have been relied upon in many aspects of Extension programming and current budget conditions have reduced the level of staffing within WVU Extension. There may be an increased demand for volunteers to help with programming. The purpose of this study was to investigate the relationship between motivation and the perceived level of well-being within the WVU Extension volunteer programs. The study yielded 464 responses from a descriptive survey. Based on the results, WVU Extension volunteers are motivated based on their …

Dynamically Scaling Biomimetic Robots Through Parallel Viscoelastic Actuators, Foster Olaf Holmquist

Graduate Theses, Dissertations, and Problem Reports (ETD)

Robots provide a unique proving ground for testing scientists’ understanding of neuromuscular systems. Synergistically, mimicking biological control laws and mechanics could offer robotics the robust and adaptive locomotion of animals. Previous work has utilized the wealth of research on insect biomechanics to develop scaled robotic models but doing so presented a problem: while these robots’ larger scale has simplified manufacturing, the scale has also shifted its mechanics from those of the modeled organisms. While animals walk, forces are generated that describe their mechanics and influence motor control. The musculoskeletal structure and stretch of muscles result in viscous and elastic forces, …