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Evaluation Of Brook Trout (Salvelinus Fontinalis) Thermal Tolerance And Projected Climate Change Effects On Headwater Brook Trout Population Persistence, Cory Bauerlien

Graduate Theses, Dissertations, and Problem Reports (ETD)

Climate change has been identified as one of the greatest threats to global biota in recorded human history. Consequences are expected to be more pronounced in ectotherms because most of their physiology is controlled by external temperature conditions. Predicting how ectotherms may respond to increasing global temperatures necessitates information about thermal limits of thermal sensitive species and how increasing temperatures may affect individual and population-level fitness. Therefore, the objectives of this research were aimed at gaining a better understanding of the mechanisms that underpin thermal tolerance limits of fish and how those mechanisms may affect fitness and population persistence under …

Multi-Omics Analysis Of Immune-Metabolic Adaptation To Direct-Fed Microbial Supplementation In Newly Weaned Beef Steers, Adekunle Odunayo Ajiboye

Graduate Theses, Dissertations, and Problem Reports (ETD)

Immune and metabolic disruption during weaning represents a major challenge to health and productivity in beef cattle. This dissertation tested the hypothesis that supplementation with multi-strain direct-fed microbials (DFMs) modulates immune regulation and metabolic pathways associated with adaptation to weaning stress. To address this, whole-blood transcriptomics and plasma metabolomics were used to evaluate molecular and metabolic responses in newly weaned beef steers during the receiving period. Transcriptomic analysis identified differential expressions of genes associated with immune signaling and inflammatory regulation, indicating modulation of immune-related pathways without changes in growth performance. Metabolomic analysis revealed alterations in circulating metabolites related to amino …

Acts Of Becoming: A Phenomenological Investigation Into The Lived Experiences Of West Virginia Teacher-Activists, 1990 To The Present, Phillip Elias Howard

Graduate Theses, Dissertations, and Problem Reports (ETD)

This critical phenomenological dissertation explores the lived realities of becoming a West Virginia teacher-activist. Presenting the experiential accounts of three participants, its semi-structured questions Show the lifeworld forces, inherited (factical) self-identities, and occupational, "on-the-ground" classroom experiences that inspired its research collaborators to assert themselves as unionists and natural teacher-organizers. This dissertation utilizes a collection of Marxian, phenomenological, and post-structural interpretation devices to develop robust, sensemaking themes which reveal the meaning of participants’ lived reflections. It also provides thick descriptions of co-researchers' historic and current political, economic, and socio-cultural backdrops amid West Virginia’s right-wing political realignment of the 2010s, the eternal …

Using Aqueous Geochemistry Of Acid Mine Drainage To Spatially Investigate Concentrations Of Rare Earth Elements Throughout West Virginia, Pennsylvania, And Maryland, Elyssa Lynn Allen

Graduate Theses, Dissertations, and Problem Reports (ETD)

Rare earth elements (REEs) are an important commodity utilized in many industries such as electronics, defense, manufacturing, medicine, and green energy production. A lack of economical domestic sources forces the United States to import a majority of its supply, and incentivizes novel methods of acquisition. Mining regions of the United States, such as Appalachia or the West, are afflicted with acid mine drainage (AMD) impacted surface waters. REEs occur in varying concentrations within coal seams, indicating that coal and its waste are potential avenues of recovery that might also incentivize the remediation of coal impacted areas. Understanding spatial and geochemical …

Studying The Mechanism For Ricin-Induced Growth Suppression, Daniel Judge

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study focuses on ribosome-inactivating proteins (RIPs), a class of potent toxins that includes ricin, α-sarcin, and shiga toxin, which irreversibly damage large subunit rRNA. Although the catalytic activity of ricin toxin A chain (RTA) is well characterized, the precise mechanisms linking ribosome damage to altered cellular morphology remain poorly understood. In this study, we engineered a tetracycline-regulatable expression system to express RTA in Saccharomyces cerevisiae. We characterized the effects of RTA expression on yeast growth, viability, and reproductive potential. We demonstrated that our expression system produced rapid and robust RTA expression and ribosome depurination within less than one …

Population Dynamics And Future Viability Of The Eastern Massasauga Rattlesnake (Sistrurus Catenatus) In Southwest Michigan, Matthew John Klein

Graduate Theses, Dissertations, and Problem Reports (ETD)

Managing at-risk species or populations requires understanding the demographic and genetic drivers of long-term population trends. The eastern massasauga rattlesnake (Sistrurus catenatus) is a federally threatened species facing multiple interacting threats across its range. Habitat loss from wetland drainage and agricultural land conversion has caused range contractions and geographic isolation of populations. Because remaining populations are often small and widely separated, most are now genetically isolated, threatening their long-term viability. Reversing these declines requires understanding population dynamics, quantifying extinction risk, identifying drivers of long-term viability, and assessing the effects of inbreeding and the potential for genetic rescue to …

Survival, Cause-Specific Mortality, Resource Selection, Movement Rates, And Population Dynamics Of White-Tailed Deer (Odocoileus Virginianus) In West Virginia, Usa, Brett P. Skelly

Graduate Theses, Dissertations, and Problem Reports (ETD)

White-tailed deer (Odocoileus virginianus) are one of the most culturally and economically important big game species in the United States. At the turn of the 20th century white-tailed deer were almost extirpated due to market hunting and unsustainable forestry practices that destroyed habitat. The creation of game laws that eliminated market hunting and created seasons and bag limits for deer hunting along with restocking efforts by sportsmen’s groups and state wildlife agencies aided in deer population recovery. These efforts have led to the abundant deer populations that are currently enjoyed by hunters and wildlife viewers. Deer populations require …

An Assessment Of Disease Risks, Population Genetics, And Spatial Ecology Of Appalachian Cottontails (Sylvilagus Obscurus) And Snowshoe Hares (Lepus Americanus) In West Virginia, Usa, Madison Leigh Miller

Graduate Theses, Dissertations, and Problem Reports (ETD)

Snowshoe hares (Lepus americanus) occupy a large range across the United States and Canada. While snowshoe hare populations are generally considered stable in the interior of their range, populations along range edges are experiencing declines, possibly driven by climate-related factors such as seasonal coat color mismatch. West Virginia represents one of the southernmost extents of the species’ range, yet little is known about the population genetic status or population sizes of snowshoe hares in the state. Appalachian cottontails (Sylvilagus obscurus) are another lagomorph species found in West Virginia. This species experienced a historical decline and currently …

Stakeholder Perceptions Of West Virginia’S Artificial Food Dye Ban In K–12 School Meals: Initial Year Of 2025-2026, Leah Michelle Rogers

Graduate Theses, Dissertations, and Problem Reports (ETD)

OBJECTIVES: Artificial food dyes (AFD+) are an increasing policy concern in the U.S., particularly in children’s foods. West Virginia’s House Bill 2354, the first statewide AFD+ ban implemented in K-12 schools, restricts seven AFD+ in state-funded meals. Little is known about how AFD+ bans are perceived or implemented. This study examined how the ban functioned within school food systems and the barriers and facilitators experienced by school stakeholders within the initial year of implementation during the 2025-2026 school year.

METHODS: A cross-sectional, observational mixed-methods study was conducted among implementers, enforcers, and receivers of the policy. A survey tool …

Surrogate-Based Approaches For Evaluating Post-Harvest Food Safety Interventions In Fresh Produce, Md Shafiul Islam Rion

Graduate Theses, Dissertations, and Problem Reports (ETD)

Pathogenic bacteria are still a threat to human beings worldwide as their presence is often unknown even if they are already present in fresh produces. This in turn resulted in several outbreaks and recalls across the United States. This dissertation investigated the survival of pathogenic bacteria and their potential non-pathogenic surrogates after antimicrobial interventions in different modes of applications on the surface of cantaloupes, butternut squash, and fresh cilantro. In the first study, peroxyacetic acid (PAA) was applied in three separate doses on nalidixic acid resistant Salmonella Typhimurium, Listeria monocytogenes, Enterococcus faecium, and Listeria innocua inoculated cantaloupe surfaces …

An Assessment Of The Reinforcing And Aversive Properties Of Transition-Related Stimuli, Lillian Louise Skiba-Thayer

Graduate Theses, Dissertations, and Problem Reports (ETD)

Transitions from relatively favorable (rich) to relatively unfavorable (lean) activities have been shown to disrupt behavior. Transition-related disruptions in behavior have been observed across laboratories, species, and procedural arrangements. The most commonly reported disruption in basic research is a pause in the reinforced behavior in the signaled transition from a relatively rich to a relatively lean condition of reinforcement (Perone & Courtney, 1992). The present study assessed the function of the stimuli associated with transitions as either reinforcing, punishing, or neutral across two experiments using eight pigeons as subjects. Experiment 1 tested the function of two stimuli, one correlated with …

A Survey Of Disability-Inclusive K-12 Public School Music Programs In The United States Where Best Buddies Friendship Programs Have Been Established, Geena Louise Jeffries Mattox

Graduate Theses, Dissertations, and Problem Reports (ETD)

This study examines the presence and structural characteristics of disability-inclusive band, orchestra, and choir programs serving students with intellectual and developmental disabilities (IDDs) in K-12 public schools across the United States that implement the Best Buddies School Friendship Program. Intellectual and Developmental Disabilities is defined as group of chronic, lifelong conditions that originate before age 22, affecting physical, learning, language, or behavior areas. Despite federal legislation, peer reviewed scholarship specifically addressing access to performance-based music ensembles for students with IDDs remain comparatively limited. Employing a survey distributed nationwide within a defined population of schools implementing Best Buddies Programs, administered via …

Unsuccessful? Universal Human Rights And The United Nations: Russian Imperialism In Ukraine And The Post-Udhr International System, Sofiia Novytska

Graduate Theses, Dissertations, and Problem Reports (ETD)

Human universality has always stood as a controversial subject and remains politically contentious today. Following the humanitarian crisis of World War II, the international community was determined to protect the integrity and dignity of people worldwide by developing the United Nations’ Universal Declaration of Human Rights. However, universal human rights were not invented by the Declaration and were already conceptually established in global ancient thought, further evolved into Early Modern scholarship, and later paved the way for modern humanitarian frameworks. The UDHR was not universally well-received, and was criticized for its revolutionary legal character, postcolonial contestation, and practical ambiguity. The …

Differences In Dry Matter Intake In Grazing And Drylot Beef Cattle, Tylor John Yost

Graduate Theses, Dissertations, and Problem Reports (ETD)

Accurate measurement of individual dry matter intake (DMI) in grazing cattle remains a major challenge for evaluating feed efficiency traits in beef production systems. This study evaluated relationships among intake and efficiency traits measured in both pasture and drylot environments and assessed the potential for predicting pasture DMI using water intake and machine learning approaches. A total of 163 beef cattle across four experimental groups were evaluated in one of two grazing seasons (2024 or 2025). Drylot feed and water intake were measured using automated intake monitoring equipment, whereas pasture intake was determined using a long short-term memory …

So There I Was, Jesse Marie Baggett

Graduate Theses, Dissertations, and Problem Reports (ETD)

This written thesis provides a description and analysis of my thesis exhibition, So There I Was, which presents a body of ceramic sculptures and paintings that explore themes of adventure, travel, and shared experiences. The work reimagines personal experiences through a cast of animal characters, with a rabbit acting as a stand-in for myself. These narratives are inspired by events from my own life and are transformed into imaginative scenes that emphasize playfulness, wonder, and storytelling.

The sculptures combine toy-like animal figures, pull carts, and miniature architectural structures to create playful environments that resemble playsets or staged scenes where …

Posttraumatic Growth In Appalachian Youth: Findings From An Outpatient Pediatric Trauma Specialty Clinic, Marinne M. Morgan

Graduate Theses, Dissertations, and Problem Reports (ETD)

Childhood traumatic event exposure (CTE) involves exposure to extreme events that overwhelm one’s ability to cope. Childhood traumatic event exposure has been linked to a wide range of adverse mental and physical health consequences, including posttraumatic stress symptoms (PTSS). Fewer studies have examined positive trajectories following childhood traumatic event exposure, such as posttraumatic growth (PTG), especially in understudied regions such as Appalachia. The current study used a cross-sectional design to examine posttraumatic growth in 14 youths aged 9 to 15 years seeking trauma-focused therapeutic services at an outpatient pediatric trauma specialty clinic in West Virginia. In line with previous research, …

Landscape As Expo: Reimagining Desert Landscapes As Climate-Adaptive Cultural Infrastructure In Las Vegas, Parinaz Baradaran Anaraki

Graduate Theses, Dissertations, and Problem Reports (ETD)

Contemporary desert cities face increasing environmental and social challenges driven by climate change, including extreme heat, water scarcity, fragmented ecological systems, and limited access to high-quality public open space. In many rapidly urbanizing regions, conventional urban development has marginalized desert landscapes, treating them as vacant land rather than valuable ecological and cultural assets. This thesis proposes Landscape as Expo as a new framework for transforming desert landscapes into climate-adaptive cultural infrastructure, where ecological processes become both functional systems and public experiences.

The project focuses on a 140-acre site within the Tule Springs Planning District in North Las Vegas, Nevada, identified …

The Histamine-3 Receptor In Cardiac Fibrosis: A Novel Role In Fibroblast Function, Heather Linsky

Graduate Theses, Dissertations, and Problem Reports (ETD)

In the United States, heart failure affects approximately 6.7 million individuals. Despite a number of available therapies, heart failure prevalence continues to grow, suggesting the need for a broader range of effective therapies. More specifically, heart failure with preserved ejection fraction comprises approximately 50% of all heart failure cases and, as the name suggests, is a disease of diastolic dysfunction as opposed to systolic dysfunction. A hallmark of heart failure with preserved ejection fraction is myocardial fibrosis, which is a key contributor to diastolic dysfunction, and is the major focus of this dissertation work. In the heart, histamine-3 receptor activation …

Macroinvertebrate Response To Two Highway Construction Projects In West Virginia, Brenden Nauman

Graduate Theses, Dissertations, and Problem Reports (ETD)

Highway development and construction are frequently required to provide efficient transportation for an increasing human population. Specific to the Appalachian region, highway development has been a major goal since the mid 1900’s, with the aim of promoting economic growth and ease of travel. While highways provide necessary services to humans, their construction and presence often impact nearby waterways through multiple mechanisms including sedimentation and urbanization. To understand the degree of impact, streams are often monitored using biological data, which can be used to quantify effects. In this work I used freshwater macroinvertebrate communities to monitor changes in stream health surrounding …

Preliminary Analysis Of Soil Volatile Organic Compounds As Soil Health Indicators, Thorne Taul

Graduate Theses, Dissertations, and Problem Reports (ETD)

Over the past decade, soil health (SH) has gained popularity due to the novelty of sustainable soil use related to human wellbeing. Soil health describes the overall quality of soil and its capacity to function over time. As interest in SH grows, researchers continue to explore new soil health indicators that can improve knowledge on how soil health is assessed and managed. Some indicators directly reflect soil health status, and some indirectly relate to processes and functions occurring within the soil. Volatile organic compounds (VOCs) are naturally occurring compounds that play a role in multiple soil processes. They are associated …

Genomic Variation, Adaptive Evolution, And Invasive And Evolutionary History Of Microstegium Vimineum, Cameron William Corbett

Graduate Theses, Dissertations, and Problem Reports (ETD)

Invasive species can cause environmental and economic damage, but can offer unique opportunities to study rapid evolution and adaptation in novel environments. Microstegium vimineum (stiltgrass) is a prolific invasive grass in the eastern United States. Historical herbaria collections were used to identify patterns of invasion history and population dynamics over a spatial and temporal scale. Analyses of plastid genomes reveal a complex invasion history, including a single introduction in the southern United States linked to Japan and multiple repeated introductions in the northern United States from various source populations. Spread of introduced populations provide evidence of admixture among previously geographically …

Treatment Of Birdsong In Selected Works For Solo Piano, Madeline Hoover

Graduate Theses, Dissertations, and Problem Reports (ETD)

Birdsong-inspired music makes up a small but important part of the classical piano repertoire. This paper reviews birdsong in classical music since the seventeenth century, exploring different techniques for mimicking birdsong and the unique strengths of the piano as an instrument for purveying bird music. In the Baroque and Classical eras, the cuckoo song was sampled often because of its simplicity. Later, composers’ attention turned toward more complicated songs, like that of the hermit thrush. Interest in more complex songs led to experimentation in notation and a push for more accurate transcriptions. In the 20th century, composers included recordings of …

Ornithine Metabolism In Retinal Pigment Epithelium And Retina And Implications In Gyrate Atrophy, Artjola Puja

Graduate Theses, Dissertations, and Problem Reports (ETD)

Ornithine aminotransferase (OAT) is a mitochondrial enzyme that regulates ornithine metabolism by converting ornithine to pyrroline-5-carboxylate, a precursor for proline and glutamate synthesis. Deficiency of OAT causes systemic ornithine accumulation and leads to gyrate atrophy (GA) of the choroid and retina, a hereditary disorder characterized by progressive chorioretinal degeneration and irreversible vision loss. Chorioretinal atrophy typically starts at the mid-peripheral retina and progresses toward the macula. Although elevated ornithine is considered toxic to the eye, how ornithine is metabolized in ocular tissues and why OAT deficiency causes selective, region-specific vulnerability remain unclear. We combined ex vivo and in vivo stable …

Process Optimization And Quality Control In Manufacturing Of Frp Composites By Vartm, Mohamed Emadeldin Omar Abdelhadi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Railroad tank cars, being one of the most used means of transportation of hazardous material across the country, have not been structurally upgraded for a long time. Thus, the Pipeline and Hazardous Materials Safety Administration (PHMSA), a United States Department of Transportation (USDOT) agency, released a rule for safer transportation of highly hazardous and flammable liquids by railroad to enhance the design specifications of both the new and existing tank cars in May 2015. A multifunctional composite jacket is proposed as a new material to structurally enhance the railroad tank cars to withstand any accidents that could happen en route. …

Do You Walk On By? The Effects Of Donor Fatigue On Willingness To Donate, Shawn Michael Henry

Graduate Theses, Dissertations, and Problem Reports (ETD)

Donation solicitation literature validates the successful usage of various solicitation tactics that lead to achieving set donation goals for their associated cause. Yet, while the financial success of “checkout charity” is well-documented, a significant gap remains regarding the impact of high-frequency solicitations on long-term consumer-brand relationship. This research addresses this gap. Over the span of three surveys, this study finds that donation request frequency overall erodes donation likelihood through donor fatigue when donation frequency changes (occasional vs. always). However, this relationship is moderated by the perceived consumer base, with a larger perceived consumer base exacerbating the “diffusion of responsibility.” Further, …

Comparison Of Push-Pull Air Curtain To Overhead Downdraft Systems On Aerosol Containment And Distribution In Controlled Environments, Manal Mutairi

Graduate Theses, Dissertations, and Problem Reports (ETD)

Abstract

Comparison of Push-Pull Air Curtain to Overhead Downdraft Systems on Aerosol Containment and Distribution in Controlled Environments.

This dissertation looks at the ongoing issue of tiny airborne particles created during medical and dental procedures and evaluates ways to reduce the risk of exposure for healthcare workers and patients to these particles. The first study focuses on the size, how long particles stay in the air, and how far they travel from common medical procedures that create aerosols, showing that tiny particles (less than 5 µm) can stay in the air long enough to escape basic droplet controls. As a …

Increasing Documentation Of Endotracheal Tube Cuff Pressures: A Quality Improvement Initiative For Flight Crew Providers, Matthew D. Noss

Graduate Theses, Dissertations, and Problem Reports (ETD)

ABSTRACT

Background: There was a lack of documentation of endotracheal tube (ETT) cuff pressures (CPs) by flight crew providers at Base 1 of HealthNet Aeromedical Services. A three-month pre-intervention data collection showed 1.8% of patients had initial CPs documented. Inadequate documentation and monitoring of ETT CPs can lead to patient complications such as tracheal injury and aspiration making this an important area for quality improvement.

Purpose: To increase documentation of ETT CPs through education and evidence-based interventions.

Intervention: This project implemented an educational in-service on ETT cuff syringe manometry for flight crew providers. The educational intervention consisted of …

Mechanisms Of Glyphosate Toxicity: Uptake, Mitochondrial Function, And Metabolism, Dionysios Patriarcheas

Graduate Theses, Dissertations, and Problem Reports (ETD)

Glyphosate, the most widely used herbicide in the world, is an inhibitor of chorismate biosynthesis, the precursor of aromatic amino acids. The target of glyphosate is absent in animals, but findings reporting toxicity in model organisms without a known mechanism or target are increasing. Saccharomyces cerevisiae is a useful and versatile model for understanding molecular mechanisms of toxicity. In yeast, previous work has shown that the aspartate/glutamate permease Dip5 is the proposed transporter for glyphosate and that mitochondrial metabolism has been implicated in response to glyphosate toxicity. This dissertation aimed to identify the effects of glyphosate on mitochondrial function through …

Multi-Omic Characterization Of Immune And Metabolic Responses To Inflammatory Challenges In Sheep, Samanthia Raceine Johnson

Graduate Theses, Dissertations, and Problem Reports (ETD)

To meet the demands of the growing population, it is imperative to develop sustainable and applicable ways to improve animal health and productivity. The first step in doing so is understanding the mechanisms that govern health and immune response and find ways to appropriately modulate them to improve animal health and productivity. The objective of this study is to use multi-omic techniques to understand breed-specific immune responses in sheep that may contribute to their resistance and susceptibility to parasites and to a greater extent, immunocompetence. Known parasite-resistant breed, St. Croix (STC) were compared to parasite susceptible Suffolk (SUF) sheep for …

Video-Based Heart Failure Education: A Feasibility Study, Avery Pivont

Graduate Theses, Dissertations, and Problem Reports (ETD)

Video-Based Heart Failure Education: A Feasibility Study

Avery Pivont

Background:  Heart failure (HF) is a leading cause of hospitalization in the U.S., contributing to high readmission rates and significant healthcare costs. Many of these readmissions are preventable and often stem from inadequate patient education. Effective patient education is a critical component of HF management; however, many healthcare facilities lack specialized HF nurse educators, resulting in inconsistent patient education.

Problem Statement: A quality improvement team at a large academic hospital identified increased heart failure (HF) readmission rates across an entire health system, largely attributed to inconsistent discharge education and limited availability …