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Graduate Student Theses, Dissertations, & Professional Papers

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An Exploratory Analysis Of The Role Of Sex In Load Carriage, Rachel E. Perez, Dustin R. Slivka, Brent C. Ruby Jan 2026

An Exploratory Analysis Of The Role Of Sex In Load Carriage, Rachel E. Perez, Dustin R. Slivka, Brent C. Ruby

Graduate Student Theses, Dissertations, & Professional Papers

Axially loaded weight may impose a metabolic demand equivalent to that of fat mass (FM) during load carriage. Males (M) typically have a lower body fat percentage than females (F), resulting in metabolic demand differences during load carriage that may be mitigated by accounting for FM in pack loads. PURPOSE: To determine whether normalizing pack load to FM equalizes metabolic cost across sexes during load carriage. METHODS: Twenty-eight participants (14M, 23 ± 5 y, 1.81 ± 0.07 m, 78.7 ± 10.0 kg, 12.7 ± 5.4% body fat, VO2peak 4.18 ± 0.54 L·min-1; 14F, 22 ± 4 …


Velocity Based Training In Athletic Development, Ross Martin Sangalang Jan 2026

Velocity Based Training In Athletic Development, Ross Martin Sangalang

Graduate Student Theses, Dissertations, & Professional Papers

The foundation of high-level sport performance is the ability to produce and express force rapidly across the task-specific temporal demands of sport. Traditional percentage-based prescription methods have long been used to develop hypertrophy, maximal strength, and power; however, fixed %1RM prescriptions fail to account for daily undulations in athlete readiness, accumulated fatigue, and changes in neuromuscular capacity. Velocity based training (VBT) offers a practical framework to regulate training through measurement of concentric velocity, allowing coaches to more accurately prescribe loads, stringently monitor repetition quality, and precisely regulate fatigue. This review discusses the neuromuscular qualities underpinning success in athletic endeavors, limitations …


Probing Proficiency-Related Neural Representations With Pca And Ica, Onila R. Narayana Mudalige Don Jan 2026

Probing Proficiency-Related Neural Representations With Pca And Ica, Onila R. Narayana Mudalige Don

Graduate Student Theses, Dissertations, & Professional Papers

How proficiency-related information is represented in task fMRI depends not only on the data themselves, but on the representational lens used to summarize them. This thesis examines second-language (L2) proficiency as a problem of representational organization rather than as a simple classification exercise. Using task fMRI from adult language learners performing semantic animacy judgments in their native language (L1) and second language (L2), I derive shared low-dimensional network representations with principal component analysis (PCA) and independent component analysis (ICA), then evaluate those representations under matched leakage-controlled decoding pipelines.

Across analyses, the central comparison is between representational frameworks rather than between …


Establishing Metric Method’S To Identify Variation In Human Biological Sex Using The Upper And Lower Limb Bones Of Non-Population Specific Samples., Roland P. Sanchez Jan 2025

Establishing Metric Method’S To Identify Variation In Human Biological Sex Using The Upper And Lower Limb Bones Of Non-Population Specific Samples., Roland P. Sanchez

Graduate Student Theses, Dissertations, & Professional Papers

Sex estimation of human skeletal remains is a facet of the biological profile. The pelvis has been suggested as the best source to accurately predicting the biological sex, but not always available after the recovery of human remains. At times, only long bones of human skeletal remains are recovered for identification. It has been shown that metric measurements using long can accurately predict biological sex. The most common methods use discriminant functions to predict the likelihood an individual falls within a certain population. These studies rely on population specific collections of skeletal remains for increased accuracy. It’s advised to avoid …


Aspen Stands Enrich Bird Communities Following A Wildfire In A Montane, Mixed Conifer Forest, Cody Q. Lane Jan 2025

Aspen Stands Enrich Bird Communities Following A Wildfire In A Montane, Mixed Conifer Forest, Cody Q. Lane

Graduate Student Theses, Dissertations, & Professional Papers

Fire is a major ecological force in most terrestrial systems, but the responses of animal communities to fire vary markedly across ecosystems due to variability in fire regimes and vegetation communities. Quaking aspen (Populus tremuloides) is unique within coniferous forests of western North America for its ability to regenerate following wildfire via vegetative resprouting. As managers employ techniques to restore fire regimes and conserve declining aspen forests, it becomes important to understand how aspen alters postfire communities. Our objectives were to (1) assess how aspen interacted with fire severity and years since fire to influence avian species and …


The Relationship Between Principals’ Transformational Leadership And Teachers’ Organizational Citizenship Behavior Within Public Primary And Middle Schools In Baotou, Inner Mongolia, China, Chuxuan Yin Jan 2025

The Relationship Between Principals’ Transformational Leadership And Teachers’ Organizational Citizenship Behavior Within Public Primary And Middle Schools In Baotou, Inner Mongolia, China, Chuxuan Yin

Graduate Student Theses, Dissertations, & Professional Papers

This non-experimental causal-comparative study was designed to explore the relationship between principals’ transformational leadership and teachers’ organizational citizenship behavior within public primary and middle schools in Baotou, Inner Mongolia, China, as well as the differences in principals’ transformational leadership and teachers’ organizational citizenship behavior based on demographic characteristics. A sample of 142 principals and 675 teachers was recruited. Data collection utilized two validated instruments: the Transformational Leadership Questionnaire for evaluating principals’ transformational leadership level, and the Organizational Citizenship Behavior Scale for Primary and Middle School Teachers for measuring teachers’ organizational citizenship behavior level. Both descriptive statistics (frequency distribution, mean, standard …


Positive Allosteric Modulation Of Glun1/Glun3 Nmda Receptors, Mia Ruth Johns Jan 2025

Positive Allosteric Modulation Of Glun1/Glun3 Nmda Receptors, Mia Ruth Johns

Graduate Student Theses, Dissertations, & Professional Papers

N-methyl-D-aspartate (NMDA) receptors are ionotropic glutamate receptors that mediate excitatory neurotransmission in the central nervous system (CNS) where they play critical roles in normal and pathological brain functions and neurodevelopment. While the glutamate/glycineactivated GluN2-containing NMDA receptors (GluN1/GluN2) have been extensively studied, the physiological roles and pharmacology of glycine-activated GluN3-containing receptors (GluN1/GluN3) remain less understood. Although GluN1/GluN3 receptors exhibit unique functional properties and play distinct roles in neuronal development and synapse maturation, studies of their precise roles in neurophysiology and circuit function are impeded by limited availability of GluN3-selective pharmacological tools. This study describes UCM-A86, a novel GluN3-selective positive allosteric …


Small Mammal Population Dynamics And Torpor Use In A Changing Subnivean Environment, Derik Butts Jan 2025

Small Mammal Population Dynamics And Torpor Use In A Changing Subnivean Environment, Derik Butts

Graduate Student Theses, Dissertations, & Professional Papers

Despite global temperature increases, winter soil surface temperatures in some northern regions might actually be getting colder due to diminishing snowpacks exposing the soil surface to ambient winter conditions. Small mammal species in northern temperate areas often utilize a microclimate beneath the snow, known as the subnivium, to escape harsh winter conditions. Exposure to winter conditions without the subnivium as a buffer could cause small mammals to experience physiological stress and population declines. In this thesis, I explore the impacts of a declining subnivium from two complementary perspectives. In chapter 1, we used a structural equation model (SEM) to test …


Hormonal Decay After Death: Evaluating Dhea-S As A Potential Molecular Marker For Postmortem Estimation, Marisa Lydia Novak Jan 2025

Hormonal Decay After Death: Evaluating Dhea-S As A Potential Molecular Marker For Postmortem Estimation, Marisa Lydia Novak

Graduate Student Theses, Dissertations, & Professional Papers

Post-mortem interval (PMI) estimation is a central component of forensic anthropological investigation, but traditional methods for estimating time since death lose accuracy after ~72 hours. Recent research indicates that some steroid hormones may undergo predictable degradation patterns after death, offering potential use as biochemical clocks for PMI estimation beyond this threshold. Dehydroepiandrosterone (DHEA), and its sulfate form (DHEA-S) are of particular interest due to their metabolic stability, systemic influence, and association with trauma. This study investigates whether DHEA-S hormonal degradation patterns correlate with time elapsed since death and body temperature, evaluating their potential as biochemical PMI markers. 160 mL of …


In The Clear: Timber Harvest Informs Elk Summer Nutrition And Resource Selection In A Densely Forested Landscape, Trevor C. Weeks Jan 2025

In The Clear: Timber Harvest Informs Elk Summer Nutrition And Resource Selection In A Densely Forested Landscape, Trevor C. Weeks

Graduate Student Theses, Dissertations, & Professional Papers

As wildlife and habitats face unprecedented challenges in the form of landscape conversion, shifting disturbance regimes, and climate change, it becomes increasingly important for land and wildlife managers to find avenues for collaboration as they seek to maintain habitat to support robust wildlife populations. Timber harvest may provide one such avenue for collaboration. It has been shown to increase the quality and quantity of ungulate nutritional resources by reverting stands to early successional stages. Additionally, timber harvest may allow for a more precise application of such effects, as managers choose where and how to harvest timber. However, the exact effects …


An Acceptable Time, Crystal Mccallie Jan 2025

An Acceptable Time, Crystal Mccallie

Graduate Student Theses, Dissertations, & Professional Papers

This scholarly work is my interpretation of the art in the exhibition, An Acceptable Time, which ecompasses a diversity of media that varies as much in material and process as the span of my life as mother, a steward of the domestic home, and a spiritual child-at-heart myself. At the center of the work is a contemplative beauty inviting the viewer to linger with the art and reflect upon the echoes of the past as they might affect the present. The formal delivery of illustrative realism, a mirage of pattern, and abstract layering draws out major themes of memory, nostalgia, …


Umfc #132: A Comprehensive Forensic Case Report, Allison Paige Mitchell Jan 2025

Umfc #132: A Comprehensive Forensic Case Report, Allison Paige Mitchell

Graduate Student Theses, Dissertations, & Professional Papers

This case report of an individual designated as University of Montana Forensic Case 132 (UMFC 132) serves to partially fulfill the requirements for the Master of Arts (MA) degree in Forensic and Biological Anthropology. UMFC 132 is a complete human skeleton, used as part of the teaching collection in the University of Montana Forensic Anthropology Laboratory. The minimum number of individuals (MNI) of UMFC 132 is 1. The analysis of UMFC 132 was conducted according to bioarchaeological and forensic anthropological standards of skeletal assessment. UMFC 132 was estimated to be a mid-adult, between the ages of [30-40 years] of age …


A Social-Ecological Justice Approach To Ecotourism: Examples From Cockpit Country, Jamaica, Laurel Ann C. Curry Jan 2025

A Social-Ecological Justice Approach To Ecotourism: Examples From Cockpit Country, Jamaica, Laurel Ann C. Curry

Graduate Student Theses, Dissertations, & Professional Papers

Ecotourism aims to support local social, ecological, and economic systems sustainably, but its implementation often perpetuates social and environmental injustices. This work applies a new social-ecological justice (SEJ) framework to ecotourism, combining Pope et al.’s holistic approach with principles like ecological stewardship, economic repositioning, and collaboration tailored to specific contexts. Importantly, basic capabilities for full functioning of humans and other species were used in this research to measure areas of justice and injustice. Using a case study approach with the Southern Trelawny Environmental Agency (STEA) in Jamaica's Cockpit Country, co-created interview data were analyzed to evaluate its ecotourism program’s alignment …


Evaluating The Accuracy Of Stature Estimation From Tibial Radiographs Using Known And Newly Developed Regression Formulas, Atlantis Kae Baron Jan 2025

Evaluating The Accuracy Of Stature Estimation From Tibial Radiographs Using Known And Newly Developed Regression Formulas, Atlantis Kae Baron

Graduate Student Theses, Dissertations, & Professional Papers

This study assesses the accuracy of stature estimation from tibial radiographs using digital measurements as an alternative to traditional osteometric tools. Stature estimation is crucial in forensic anthropology for identifying individuals, especially cases involving skeletal remains. Traditional methods rely on physical measurements and established regression formulas, such as those by Trotter & Gleser (1958).

This research analyzed tibial radiographs from the Forensic Anthropology Skeletal Trauma (FAST) database with ImageJ software to measure tibial lengths digitally. The sample included 59 tibiae, with measurements compared against three regression formulas; Trotter & Gleser (1958), Simon et al. (2023), and a new regression formula …


Juvenile Arthritis, Body Image, And Disordered Eating: An Exploration Of Experiences, Katelyn Ann Melcher Jan 2025

Juvenile Arthritis, Body Image, And Disordered Eating: An Exploration Of Experiences, Katelyn Ann Melcher

Graduate Student Theses, Dissertations, & Professional Papers

This qualitative study examined eating and body experiences among youth with juvenile arthritis (JA). JA, an autoimmune condition affecting nearly 300,000 youth in the United States, poses significant challenges including chronic pain, disability, and psychological concerns such as anxiety and depression. Adolescence is a critical developmental time for body image and disordered eating concerns to emerge. However, no qualitative research has explored the interactions of JA, body image, and eating behaviors among youth. Twelve youth with a JA diagnosis participated in a semistructured qualitative interview examining their experiences with JA and their feelings about their bodies and food as they …


Binoculars To Bytes: Development And Field Validation Of An Ai-Driven System For Avian Monitoring, Christian J. Dupree Jan 2025

Binoculars To Bytes: Development And Field Validation Of An Ai-Driven System For Avian Monitoring, Christian J. Dupree

Graduate Student Theses, Dissertations, & Professional Papers

Autonomous camera-trap arrays coupled with artificial-intelligence (AI) vision can lift bird monitoring beyond the spatial, temporal, and labor limits of traditional field surveys. We present Binoculars to Bytes (B2B), an open-source pipeline that turns 180–360° time-lapse imagery into analysis-ready avian data. At Freezout Lake Wildlife Management Area (Montana, USA) the system ran four-hour morning deployments during spring and fall migrations (2023–2024). A YOLO-NAS detector, incrementally refined with a “Specialized Localized Iterative Model” workflow, quadrupled local accuracy and, after confidence-based species-binning, cut false-positive rates in half. Daily AI species lists were benchmarked against contemporaneous eBird citizen-science checklists and recovered ≥ …


Mountain Pine Beetle Host Tree Selection And Climate-Growth Dynamics In Whitebark Pine, Hannah Rose Alverson Jan 2025

Mountain Pine Beetle Host Tree Selection And Climate-Growth Dynamics In Whitebark Pine, Hannah Rose Alverson

Graduate Student Theses, Dissertations, & Professional Papers

Recent climate-driven outbreaks of mountain pine beetles (Dendroctonus ponderosae) in whitebark pine (Pinus albicaulis) have raised fears of increasing whitebark pine mortality with increased warming and drought due to climate change. However, little is known about how whitebark pine responds to climate, what characteristics may predict whitebark pine susceptibility to mountain pine beetle outbreaks, or how these may interact. This information is critical in understanding how whitebark pine might persist in a changing climate. My research investigated how whitebark pine growth responds to various climate variables and how these responses may be predictive of attack by …


Taking God's Country: The University Of Montana, Morton J. Elrod, And Allotment On The Flathead Reservation, Dylan Tate Yonce Jan 2025

Taking God's Country: The University Of Montana, Morton J. Elrod, And Allotment On The Flathead Reservation, Dylan Tate Yonce

Graduate Student Theses, Dissertations, & Professional Papers

In the early decades of the twentieth century, the University of Montana (UM) developed from a small frontier college into an influential research institution. During its maturation process, the University relied on the acquisition of land to expand. In addition to its main campus in Missoula, the university sought land for research and recreation. When Congress passed the Flathead Allotment Act (1904), UM became one of many non-Native beneficiaries of federal land dispossession policy in the United States. Existing scholarship has largely homed in on “land grant” universities established through the Morrill Act (1862), which effectively dispossessed ten million acres …


College Athlete Workload Management To Improve Performance, Brody Reid Jan 2025

College Athlete Workload Management To Improve Performance, Brody Reid

Graduate Student Theses, Dissertations, & Professional Papers

Load management is an often used and misunderstood term within the realm of sport science. It has been described as the reason behind numerous soft tissue injuries as well as being the reason star athletes sit on the bench for games. This review aims to synthesize the existing literature regarding the workload management paradox within sport. Seminal work in this topic has been gathered mostly by Tim Gabbett in the paper “The Injury Prevention Paradox” where a relationship between fitness gain and fatigue accrued is outlined. This theory was first proposed by Roger Bannister in 1975. This paper …


Feminine Felons: Violent Offenders' Intersectional Experiences In Twentieth-Century Montana, Cassidy Ann Vander Voort Jan 2025

Feminine Felons: Violent Offenders' Intersectional Experiences In Twentieth-Century Montana, Cassidy Ann Vander Voort

Graduate Student Theses, Dissertations, & Professional Papers

This paper explores the treatment of violent female offenders in Montana in both the media and prison system from 1900-1960. Women of all races faced gender discrimination from both the carceral system and popular media. Local newspapers sensationalized crimes committed by female offenders, portraying them as women who lived outside of the expectations of womanhood in the twentieth century. Newspapers and courts placed women into two categories: “mad/sad” female offenders, who received forgiveness for their crimes; or unforgivable, “bad” offenders. Crime did not determine these categorizations; instead, gender expectations of the white middle class shaped female criminals’ treatment. If these …


"I'Ve Never Felt A Pain Like This" A Dialogic Analysis Of Parent-Child Estrangement Narratives On Reddit, Grant W. Lane Jan 2025

"I'Ve Never Felt A Pain Like This" A Dialogic Analysis Of Parent-Child Estrangement Narratives On Reddit, Grant W. Lane

Graduate Student Theses, Dissertations, & Professional Papers

How do adult children estranged from their parent(s) create meaning and make sense of their estrangement in Reddit posts on the subreddit r/EstrangedAdultChild? This study analyzed Reddit posts written by estranged adult children for a presumed audience of other estranged adult children. Using relational dialectics theory (RDT) as a lens for analysis, this research examined the competing discourses that influence the meaning-making and sense-making processes of estranged adult children in their posts when the intended audience is fellow estranged adult children. Seventy-four posts from r/EstrangedAdultChild, authored by adult children who self-identified as estranged from at least one parent, were collected …


Testing The Hefner (2009) Ancestry/Population Affinity Estimation Method On Japanese Bioarchaeological Populations And Observing Changes In Trait Frequency Distribution Over Time, Tristan Charnock Hershberger Jan 2025

Testing The Hefner (2009) Ancestry/Population Affinity Estimation Method On Japanese Bioarchaeological Populations And Observing Changes In Trait Frequency Distribution Over Time, Tristan Charnock Hershberger

Graduate Student Theses, Dissertations, & Professional Papers

Population affinity is the most controversial aspect of the biological profile to assess for forensic anthropologists due to an array of factors, both historical and in application. This has led to many calls for the abandonment of this type of estimation, however, many have challenged this by arguing for the category’s importance in the death investigation process. While the abandonment of population affinity estimation is unlikely, that does not mean that current approaches to population assessment should not be reassessed. This study attempts to reevaluate the category by applying an evolutionary lens to population affinity assessment. This project applied a …


Love At First Words: A Communication Competency Approach To Romantic Relationship Initiation, Grace E. Wiegand Jan 2025

Love At First Words: A Communication Competency Approach To Romantic Relationship Initiation, Grace E. Wiegand

Graduate Student Theses, Dissertations, & Professional Papers

Initial interactions are essential to romantic relationship formation, and both parties must desire a second interaction after an initial encounter for the relationship to advance. Many studies have explored the communication styles and tactics that are effective in a person desiring a second interaction from a dating initiation scenario. However, existing research has yet to explain why some communication strategies do or do not result in second interactions. The current study seeks to examine this phenomenon further, testing the extent to which perceived partner communication competence influences a person to want to see another again after an initial interaction. In …


An Interdisciplinary Unit For Today's Learners: Integrating Science, English, And Art To Teach Climate Change, Sydney M. Ruby-Pasternak Jan 2025

An Interdisciplinary Unit For Today's Learners: Integrating Science, English, And Art To Teach Climate Change, Sydney M. Ruby-Pasternak

Graduate Student Theses, Dissertations, & Professional Papers

As today’s global challenges grow increasingly complex, interdisciplinary thinking is essential for students to make meaningful connections in their learning. Despite this need, high school students continue to have educational experiences in one-subject classrooms, often preventing them from engaging in integrated learning models. This professional project addresses the gap by developing a five-week interdisciplinary unit titled Brushstrokes of Change, which combines climate science with English language arts and visual arts to amplify student understanding of climate change concepts. This unit was designed for grades 9-12 and prioritizes 21st-Century skills in critical thinking, communication, creativity, and collaboration through …


Seahorsedad: A Study Of Metaphors In Transmasculine Birther’S Instagram Posts, Alexa Anne Runnion Jan 2025

Seahorsedad: A Study Of Metaphors In Transmasculine Birther’S Instagram Posts, Alexa Anne Runnion

Graduate Student Theses, Dissertations, & Professional Papers

Dominant notions of pregnancy frame gestation as a solely female process. However, the experiences of transmasculine birthers challenge this assumption, demonstrating that pregnancy is not inherently tied to womanhood but is instead shaped by social and cultural narratives and representations. This paper explores the unique pregnancy and parental exercises of transmasculine birthers by examining public social media posts under the hashtag #seahorsedad on Instagram. Utilizing metaphorical criticism, I aim to uncover how transmasculine birthers, known within the community as “seahorse dads,” both uphold and resist traditional notions of gender, pregnancy, and parenting through a combination of textual and visual metaphor. …


Unravelling The Physiological Impacts Of Fire On Western U.S. Conifers, Charlotte Chiara Reed Jan 2025

Unravelling The Physiological Impacts Of Fire On Western U.S. Conifers, Charlotte Chiara Reed

Graduate Student Theses, Dissertations, & Professional Papers

Wildfires are inherently spatially heterogenous, with many trees within larger burned areas not immediately killed by fire. These surviving trees often sustain some level of fire injury, yet we lack a mechanistic understanding of how these injuries affect post-fire tree physiological function, thus affecting whether trees go on to recover or die. Current hypotheses differentiate effects of fire on tree carbon balance and hydraulic function, yet critical uncertainties remain about the relative importance of each, how they interact, and whether these impacts make trees more vulnerable to post-fire disturbances like drought. Via field experiments utilizing prescribed fires and two widespread …


The Impact Of Ambient Air Quality On Asthma Outcomes In California, Abigail Obour Jan 2025

The Impact Of Ambient Air Quality On Asthma Outcomes In California, Abigail Obour

Graduate Student Theses, Dissertations, & Professional Papers

This study investigates the relationship between ambient air quality and asthma-related health outcomes in California, focusing on emergency department visits and hospitalizations from 2005 to 2022. Despite California’s strict air quality standards, individuals still face significant pollution challenges contributing to poor respiratory health, particularly among vulnerable populations. Therefore, this study looks at how fine particulate matter (PM2.5) affects asthma outcomes using panel data from 37 counties. A fixed effects regression model is employed to control for unobserved heterogeneities that are constant over time but vary across individual counties, ensuring robust estimates of the impact of pollution on health outcomes. This …


The Filament, Elena Skibicki Jan 2025

The Filament, Elena Skibicki

Graduate Student Theses, Dissertations, & Professional Papers

The Filament weaves together the results of a neurodivergent person exploring their universe, in all its harrowing beauty and familiar tragedy. The speaker traverses oceans and thunderstorms, wades through the swampy shallows of their high school, participates in a pharmaceutical supply chain and its subsequent ransacking, floats through altered mental states, and retells legends, spanning many styles while still using the lyric “I”. Scrying into a nebulous future, the speaker returns with stories of the planet and its people, and the kind of world they want to build.

The speaker exposes their ghosts throughout the collection, hoping that the adage, …


Eros And The Sublime: Tools For Reaching Toward An Altered Ecological Consciousness, Aineka Carlson Jan 2025

Eros And The Sublime: Tools For Reaching Toward An Altered Ecological Consciousness, Aineka Carlson

Graduate Student Theses, Dissertations, & Professional Papers

This thesis explores connections between the Kantian sublime and Anne Carson’s ideas regarding “eros.” Since Kant’s Critique of Judgement was published in 1790, the Kantian sublime has grown in popularity and been utilized by theorists and creative writers alike. While the Kantian sublime is often associated with nature in popular culture, there are certain egocentric and phallocentric elements of this mode of thinking that might inhibit a biocentric mindset. Through a character study of Oryx from Margaret Atwood’s novel Oryx and Crake, this thesis draws relations between the subjugation of women, nature, and the ways in which the popularized …


Structural Optimization And Stability Enhancement Of Uba(2) And Ntl9 Using The Computational Tool: Emcast, Bahar Ghazi Esfahani Jan 2025

Structural Optimization And Stability Enhancement Of Uba(2) And Ntl9 Using The Computational Tool: Emcast, Bahar Ghazi Esfahani

Graduate Student Theses, Dissertations, & Professional Papers

This study enhances protein stability prediction by refining EmCAST (Empirical C-Alpha Stability Tool), a computational tool used to identify stabilizing mutations in protein domains. We applied EmCAST to two model proteins: the α-helical ubiquitin-associated domain 2 (UBA(2)) and the α/β N-terminal domain of ribosomal protein L9 (NTL9). Protein stability is critical for proper function, and mutations can lead to misfolding and disease. To validate EmCAST’s predictions, guanidine hydrochloride-induced denaturation was monitored using circular dichroism (CD) spectroscopy for both wild-type and mutant variants. Results guided refinements to EmCAST’s algorithm, incorporating new experimental datasets to improve accuracy. Additionally, we examined the assumption …