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Genetic And Environmental Factors Shaping Precision Medicine With Rural And Tribal Populations In Montana, Jack William Staples

Graduate Student Theses, Dissertations, & Professional Papers

Precision medicine aims to optimize healthcare based on individual genetic, environmental, and lifestyle factors, offering the potential to reduce health disparities. However, rural and Tribal communities remain underrepresented in precision medicine research, which threatens to exacerbate existing health inequities. My dissertation research aims to reduce health disparities in rural and Tribal communities in Montana through community-based participatory research (CBPR) and genetic studies. We partnered with research participants of the Confederated Salish and Kootenai Tribes (CSKT) on the Flathead Reservation and Shodair Children’s Hospital (Shodair) to conduct two primary studies.

In the first study, we investigated genetic and seasonal determinants of …

Parent And Guardian: An Interpretative Phenomenological Analysis Of Sexual And Gender Minority-Affirming Caregivers, Kelly Marie Davis

Graduate Student Theses, Dissertations, & Professional Papers

Studies consistently demonstrate that sexual and gender minority (SGM) youth experience mental health disparities when compared to their heterosexual, cisgender peers due to identity-based stressors (Meyer, 2003). Given the startling rates at which this population experiences mental health challenges, there has been an ever-increasing focus on prevention and intervention efforts to promote resilience and well-being among SGM youth. Over the past several decades, scholars studying resilience in development have identified the importance of effective caregiving in buffering against adversity. However, little is known about the specific practices that caregivers of SGM youth might employ to strengthen their relationships and to …

Leaving Them In Stitches: Novel Methods For Splice-Aware Sequence Annotation, Alexander Nord

Graduate Student Theses, Dissertations, & Professional Papers

Pre-mRNA intron splicing is essential to the production of many eukaryotic proteins, but it is relatively uncommon for sequence analysis tools to account for this process in their methods. In this dissertation I present three tools for biological sequence analysis, demonstrating the benefits that they derive from their explicit awareness of intron splicing. First, I present Mirage2, an updated implementation of the Mirage multiple sequence alignment program, which produces alignments of protein isoforms according to spliced genome mapping coordinates. I then present Diviner, which synthesizes the output data produced by Mirage2 to locate novel coding regions on eukaryotic …

Integrating Individual And Species Traits To Better Identify Risks To Persistence And Survival In Amphibian Populations, Ross Kenneth Hinderer

Graduate Student Theses, Dissertations, & Professional Papers

Amphibians have experienced major declines and are highly vulnerable to the impacts of climate change. Yet we still lack sufficient information to understand where amphibians are most vulnerable to changes in climate and how effects might manifest. In this dissertation, I worked to understand risks due to climate change and investigate effective indicators of survival in amphibians. To predict which species might be most affected by increasingly dry conditions, in Chapter 1, I extended the climate change vulnerability assessment framework to rank amphibian vulnerability based on projected climate change and species life history traits. Traits including clutch size, daily activity …

Shaping Outdoor Recreation In Protected Areas: Insights From Factors Influencing Brazilian Protected Area Professionals, Renata Burlamaqui Bradford

Graduate Student Theses, Dissertations, & Professional Papers

The growing demand for outdoor recreation (OR) in protected areas has led to positive and negative impacts on natural and social environments. As stewards of these areas, protected area professionals (PAPs) are responsible for planning and managing this complexity, and yet we know little about the factors influencing their decisions and actions. This study fills this gap by examining how psychological and organizational aspects influence PAPs’ decisions and behavioral intentions toward OR in protected areas. Insights come from the PAPs working at the Chico Mendes Institute for Biodiversity Conservation (ICMBio), the Brazilian federal agency. ICMBio’s evolving approach toward OR provides …

Sequence Similarity Search Optimization: Prefiltering Strategies And Exact Matching Techniques For Biological Sequence Data, Tim Anderson

Graduate Student Theses, Dissertations, & Professional Papers

Sequence similarity search is one of the most important tasks in the field of bioinformatics. The identification of regions of similarity between one protein or DNA sequence and a separate collection of sequences or models of sequence families has wide-ranging applications. These range from mapping of RNA sequence reads to a given genome to the annotation of gene-encoding regions and the reconstruction of evolutionary relationships of species. As genetic sequencing technologies have improved over the last few decades, the size of genetic sequence databases has grown exponentially. With this growth of sequence databases, more efficient algorithmic approaches are needed to …

Turbulence Resistant Schools: A Qualitative Phenomenological Study Of Educational Leadership In Central And Eastern Europe, Sofia Reis

Graduate Student Theses, Dissertations, & Professional Papers

The expanding complexity and increasing disruptions of our world affects schools. During both stable and unstable times, successful educational leaders support their schools’ ability to accomplish their core mission: student learning and development. Using a qualitative approach, this research explored experiences of educational leaders who established schools in Central and Eastern Europe during the post-Soviet era and continue leading them to this day. The central question was: What is the lived experience of successful assertive educational leaders as they navigate extreme turbulence? In this study, leadership is understood as a collegial activity determined by the context where it is embedded, …

Local Visions For Patagonia: Drivers And Dynamics Of Collaborative Environmental Governance In Aysén, Chile, Zachary J. Hummel

Graduate Student Theses, Dissertations, & Professional Papers

Collaborative governance (CG) has emerged as a tool for addressing increasingly complex societal problems by engaging a diverse of set of cross-sector actors in a deliberative process with the aim of realizing consensus-driven outcomes. Emerson et al.’s (2011) Integrated Framework for Collaborative Governance (IFCG) attempts to improve understanding of these arrangements by articulating causal connections between collaborative drivers and dynamics across different contexts. Answering researchers’ call for more empirical support for the IFCG in non-US/European contexts, I used the framework to guide 10 interviews with collaborative governance practitioners in the comanaged marine protected area, AMCP-MU Pitipalena Añihue, located in Aysén, …

Effectiveness Of An Evolution-Based Serious Game On Motivation And Achievement Of Secondary-Level Science Students, Bradford John Fruechte

Graduate Student Theses, Dissertations, & Professional Papers

Getting students to engage with schoolwork is not always an easy task. Lessons that have elements of games (gamified lessons) and serious games (those created for education) have the potential to increase motivation, which may also improve learning outcomes. But research has found mixed results on the achievement effects of educational games, and not enough research has shown the effectiveness of educational games on evolution-related topics specifically (Bjoerner & Hansen, 2010). This study aimed to implement a game that revolves around the mechanisms of evolution and evaluated that game’s effects on secondary-level students’ motivation and learning. Four middle school life …

A Study On The Relationship Between College Teachers’ Perception Of Professional Learning Communities And Self-Efficacy, Hongli Jia

Graduate Student Theses, Dissertations, & Professional Papers

The purpose of this quantitative survey study was to investigate the relationship between college teachers’ perceptions of the professional learning community and teacher self-efficacy. Drawing on quantitative data from a sample of 367 college teachers in Inner Mongolia in China during the 2023-2024 school year, the research investigated the impact of PLC participation on self-efficacy across different career stages and majors.

The findings revealed a medium positive correlation between college teachers’ perception in PLCs and teacher self-efficacy. The regression model indicated that collective learning and application and shared personal practice significantly influenced the overall teacher self-efficacy, but shared and supportive …

Comprehensive Case Report Umfc#140, Marymargaret C. Pratt

Graduate Student Theses, Dissertations, & Professional Papers

University of Montana Forensic Collections (UMFC) Case #140 was observed and recorded fall 2024. The information included are a complete biological profile, any taphonomic features or pathological conditions present, and a literature review on forensic odontology due to the severe nature of the teeth. This individual is consistent with a 5'2"-5'6" adult male ranging between the ages of 35-50 of ambiguous population affinity. Evidence of osteoarthritis and bone spurs are present along the vertebral spine, clavicle, and articulation points throughout the post-cranial skeleton. There is severe dental attrition found along the buccal view of the maxillary and mandibular molars. The …

A New Frontier Of Responsibility: Children's Attributions Of Moral Responsibility To Autonomous And Controlled Robots, Thomas M. Macko

Graduate Student Theses, Dissertations, & Professional Papers

A prevailing theory in moral psychology, known as the Theory of Dyadic Morality, proposes that we evaluate moral transgressions primarily through weighing the mental states and intentions of both the perpetrator and the victim of a given transgression. Previous research has found adults and children similarly refer to mental states and intentions when making moral evaluations as third-party observers of a moral transgression. How might this framework apply to social robots, who arguably lack the mental states and intentions we can effortlessly identify in humans? The present study sought to examine how children view a social robot as culpable for …

Evaluating Early-Life Characteristics And Responses Of Native Plant Materials For Dryland Restoration, Sophia N. Lasche

Graduate Student Theses, Dissertations, & Professional Papers

Globally, many drylands are in need of restoration due to anthropogenic land use changes, climate change-related biodiversity loss, and broad scale degradation. Within this degradation framework, many perennial grass species and populations have been lost from drylands. A common method of restoring vital perennial grass populations is through seed-based restoration. Using seed-based restoration, or the act of adding desirable seed to a system to restore plant populations, requires a depth of knowledge on species level responses, as well as seed sources within those species. My research examines three workhorse perennial grass species and associated seed sources for dryland restoration, especially …

Rangeland Soil Seed Bank Dynamics: Soil Property Relationships, Patterns Across A Degradation Gradient, And Responses To Shrub Management, Ryan Willis Richard Schroeder

Graduate Student Theses, Dissertations, & Professional Papers

Rangeland ecosystems are globally important for their ecosystem service delivery, however, they are experiencing several threats pushing systems across ecological states. Thus, creative science-based management is imperative to maintain ecosystem sustainability and improve ecosystem service delivery. Soil seed banks – the living seeds in the soil profile and on the soil surface – and their soil-seed-plant interactions are one area of inquiry that could help guide science-based management efforts when it comes to rangeland community assembly and ecological restoration.

In this dissertation, I investigated rangeland soil-seed-plant interactions in New Mexico, USA. In Chapter 1, I worked with an expert group …

Flyway Bottlenecks: Migratory Waterbirds Confront Diminishing Wetland Networks In Western North America, J. Patrick Donnelly

Graduate Student Theses, Dissertations, & Professional Papers

In the arid and semi-arid western U.S., migratory waterbirds (e.g., shorebirds, wading birds, and waterfowl) rely on wetland landscapes that function as nodes in broader continental habitat networks (e.g., flyways). Here, I integrate citizen science and GPS animal tracking technologies with satellite imagery and cloud computing to quantify the effects of climate and land use change on wetland ecosystems that sustain migratory waterbirds in the western U.S. Four decades of wetland surface water hydrology were first reconstructed from over 150,000 individual satellite images covering 3.1 million ha to track monthly changes in the extent and duration of wetland inundation. Results …

Moose Abundance And Calf:Cow Ratios On The Blackfeet Indian Reservation And Glacier National Park, Landon Joel Magee

Graduate Student Theses, Dissertations, & Professional Papers

Many moose (Alces alces; Sikihtsisoo) populations across North America, especially along their southern range, are declining due to factors such as climate change, parasites and disease, predation, habitat loss and degradation, and overharvest. These declines highlight broader ecological concerns, as moose play a critical role as prey within their ecosystems. Despite their importance, data on crucial demographic parameters like abundance and age ratios are sparse for moose, complicating management efforts. Conventional monitoring methods, including radio-collaring, harvest data, public surveys, and aerial surveys, face significant logistical and financial challenges, and often fail to provide a comprehensive understanding of …

Analysis Of The Transformations Of Laguna Melincué Wetland, Argentina, Bianca Giunti

Graduate Student Theses, Dissertations, & Professional Papers

In Argentina, freshwater wetlands are threatened by land use changes, unsustainable irrigation practices, and climate change. Twenty-three wetlands cover 5,176,578 ha of the Argentinian territory and have been designated as wetlands of international importance by the Convention of Wetlands (Kandus et al., 2008; Ramsar Sites Information Service, 2022). These figures are significant given Argentina’s unique geographic location on a central migratory bird flyway and the profound biological diversity found in these wetlands (Jahn et al., 2013; Red Aves Internacionales, 2015). The Government of Argentina has recently moved to implement wetland protection and conservation policies. Nevertheless, policy and governance efforts to …

Exploring Spatial Climate Analogs For Climate Impact Projection, Adaptation, And Communication, Svetlana Valeryevna Yegorova

Graduate Student Theses, Dissertations, & Professional Papers

Climate change requires that we adapt to the many impacts that are already unavoidable. To adapt, institutions and managers need to be able to anticipate how ecosystems are likely to respond to the observed and projected changes in climate. This dissertation explores spatial climate analogs for projecting climate change exposure and impacts to ecological systems, and for communicating climate impacts to diverse audiences. Chapter 1 summarizes the current state of knowledge about spatial climate analogs, and identifies areas for further research. Chapter 2 compares the skill and impact projections of analog impact models, to that of two commonly used empirical …

A Better Way To Make Protein, James Gillette Bosco

Graduate Student Theses, Dissertations, & Professional Papers

Producing recombinant proteins stands as a cornerstone in both biotechnology and pharmaceutical research, supporting the creation of therapeutics, vaccines, and enzymes used in various industries. Bacterial systems for expression, especially Escherichia coli, are preferred due to their rapid growth rates, ease of genetic manipulation, and scalability. However, generating proteins under current Good Manufacturing Practice (cGMP) standards introduces substantial challenges, such as issues with scalability, toxicity, protein misfolding, and strict regulatory demands. This section delivers an in-depth review of these obstacles and current methods developed to address them. An extensive exploration is conducted into induction techniques, including auto-induction and isopropyl-D-1-thiogalactopyranoside (IPTG) …

Evaluation Of Silk Fibroin And Hylauronic Acid As Otic Drug Delivery System Additives; Investigation Of Silk Fibroins Responses To Enviromental Stimulii, Kolton Charles Sandau

Graduate Student Theses, Dissertations, & Professional Papers

The Serban lab has previously developed and reported on a single application drug delivery system for the treatment of otitis externa. Otitis externa also known as outer ear infections is a very common ailment that impacts large portions of both human and domestic animal populations. Typical treatment for otitis externa typically requires an extended dosing regimen over several days that is inconvenient and can lead to poor clinical outcomes due to poor patient compliance. Our delivery system employs a thixotropic gel to deliver a steady dose of medication over the duration of the infection.

Here we evaluated the reproducibility and …

Quantifying Wastewater Contributions To The Upper Clark Fork River, Claire Utzman

Graduate Student Theses, Dissertations, & Professional Papers

Though algae are at the base of food webs in many rivers, large algal blooms are increasing in frequency and can cause problems with nutrient cycling, depletion of oxygen supplies at night, and release of toxic chemicals. One leading cause of algal blooms is nutrient loading into rivers, and controlling nutrient release in wastewater is one approach that can help limit algal growth. To reduce the intensity of algal blooms in the Clark Fork River in western Montana, numeric nutrient standards were put in place that regulated the release of the nutrients nitrogen and phosphorus into the river from wastewater. …

Density Anomaly Leads To Rift Initiation: Quantifying Active Rifting And Comparison To The East African Rift Zone, Chloe Boucher

Graduate Student Theses, Dissertations, & Professional Papers

Active rifting, elastic failure in the lithosphere from rising density anomalies in the mantle, is fundamental to the Wilson Cycle which describes the cycle of tectonic plates. Active rift zones are common in the oceanic lithosphere but are less common in the thicker continental lithosphere. The current largest active continental rift zone is the East African Rift Zone (EARZ). Although active rifting is fundamental to plate tectonic theory, the mechanics and parameters needed for a density anomaly to initiate a rift have not been undertaken. This model aims to solve the problem of a density anomaly initiating a continental rift …

Coalescence: A Carnivore Coexistence Curriculum That Braids Indigenous & Western Ecological Knowledge Into A Relevant And Experiential Learning Opportunity For Youth, Stephanie Anne Barron

Graduate Student Theses, Dissertations, & Professional Papers

As grizzly bears (Ursus arctos horriblis) begin to reoccupy more of their historic range, and as humans and large carnivore populations continue to increase, incidences of human carnivore conflict are on the rise. A decolonial curriculum designed in collaboration with the Confederated Salish and Kootenai Tribe’s wildlife biologists stands to increase awareness of Indigenous ecological knowledge and teach youth about the importance of coexistence with carnivores. Additionally, this project could greatly influence youth perceptions of grizzly bears and other large carnivores. This research project examines the development and implementation of a carnivore coexistence curriculum for youth that is guided by …

Three Stories On Urban Wildlife, Kevin J. Moriarty

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

Where The Framework Ends: Equity Considerations And Limitations During Agency River Permit Allocation Decision Making Processes, Kelsey Ellen Phillips

Graduate Student Theses, Dissertations, & Professional Papers

River recreation in the U.S. increased dramatically in the 1970s as boating technology and recreational skill level shifted, thus a number of federally- and state-managed river areas instituted use limits over the years to prevent the environmental and social impacts perceived to have stemmed from increased use. These river managers were then faced with the task of allocating this use between private and commercial users (allotment) and within these groups (rationing), complicated by the fact that members of the public perceive equity differently. Early recreation use allocation research explored how different rationing techniques favor different groups, for example those with …

Wildfires, Climate, And Equity: Towards Sustainable Forest Management And Conservation, Mark Regier Kreider

Graduate Student Theses, Dissertations, & Professional Papers

Forest and conservation sciences are both fields that inherently combine land and people, concerning topics such as the wildfire crisis, impacts of human-caused climate change on ecosystem functioning, and inequity of access to outdoor spaces. The research and science communication in this PhD dissertation explore these issues, integrating the biophysical and social sciences to promote understanding, stewardship, and conservation of human-forest systems. Chapter 1 assesses how humans shape patterns of fire through fire suppression, inadvertently ensuring that fires occur under the most severe conditions and effectively selecting for high-severity fire occurrences. Chapter 2 uses a wilderness area with a fire …

Bloom: A Microbial Self-Portrait, Emily Lauren Mulvaney

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

A Tale Of Two Working Landscapes, Sage C. Sutcliffe

Graduate Student Theses, Dissertations, & Professional Papers

No abstract provided.

The Impact Of Treatment Dosage On Cognitive-Linguistic Outcomes Of Patients With Aphasia: An Investigation Of Three Service Delivery Models, Adele J. Derendinger

Graduate Student Theses, Dissertations, & Professional Papers

This study addresses the gap in literature by directly comparing the effectiveness of Intensive Comprehensive Aphasia Programs (ICAPs) and modified ICAPs (mICAPs) to usual care service delivery models for people with aphasia. Through a prospective study design, cognitive-linguistic function change scores across different service delivery models were examined. The findings reveal significant within-group improvements in cognitive-linguistic function for participants in ICAPs, with some improvement seen in mICAPs and usual care conditions. Intensive models demonstrated greater improvement compared to non-intensive usual care models, particularly evident in the Western Aphasia Battery – Revised and the Boston Naming Test – Second Edition. …

Puerto Rican Haplogroup Distribution: A Taíno History, Paige Mackenzie Williams

Graduate Student Theses, Dissertations, & Professional Papers

The extent to which Puerto Rico’s indigenous communities resisted, survived, and were transformed by colonization is unclear despite extensive ethnohistorical research. Historical claims of extinction are attributed to colonial census information but are strongly opposed by islanders who claim Indigenous Taíno ancestry (Nieves-Colón et al. 2019). Ethnic composition of Puerto Ricans is primarily European, African, and Indigenous and these compositions reflect migrations and admixture. Mitochondrial DNA (mtDNA) is of vast importance, as it permits tracing of the maternal line through a common female ancestor. In this, investigation of Indigenous haplogroup frequencies and their importance and relevance in Puerto Rican genetic …