Digital Commons Network^™

Evaluating Research Centers In Minority Institutions: Framework, Metrics, Best Practices, And Challenges, Angela Sy, Traci Hayes, Kelly Laurila, Carlamarie Noboa, Robbert J. Langwerden, Michelle M. Hospital, Doris A. Andújar-Pérez, Lakesha Stevenson, Suzanne M.Randolph Cunningham, Latrice Rollins, Hala Madanat, Tanya Penn, Shiva Mehravaran

Faculty Publications

The NIH-funded Research Centers in Minority Institutions (RCMI) program is currently funding 18 academic institutions to strengthen the research environment and contribution to health disparities research. The purpose of this multiphase mixed-methods study was to establish a uniform evaluation framework for demonstrating the collective success of this research consortium. Methods included discussions of aims and logic models at the RCMI Evaluators’ Workshop, a literature review to inform an evaluation conceptual framework, and a case study survey to obtain evaluation-related information and metrics. Ten RCMIs participated in the workshop and 14 submitted responses to the survey. The resultant RCMI Evaluation Conceptual …

The Lyophilized Aqueous Leaf Extract Of Moringa Oleifera Blunts Streptozocin-Induced Diabetes In Rats Through Upregulation Of Glut 4 Signaling Pathway And Anti-Oxidant Effect, Adeolu Alex Adedapo, Iyanuoluwa Omolola Ogunmiluyi, Olufunke Olubunmi Falayi, Blessing Seun Ogunpolu, Ademola Adetokunbo Oyagbemi, Abayomi Orishadipe, Temidayo Olutayo Omobowale, Momoh Audu Yakubu, Oluwafemi Omoniyi Oguntibeju

Faculty Publications

The anti-diabetic property of aqueous leaf extract of Moringa oleifera was performed in streptozocin-induced diabetic rats using serum chemistry, histology and immunochemical parameters as indices of diabetes. The blood glucose level of the diabetic untreated group continues to increase while that of the treated group after 21 days decreased. While the animals in the diabetic untreated group experienced increase in the levels of markers of organ damage when compared to the control group (P values < 0.0001). ALT increased from 61.83±1.5 to 96.1±22.4, AST was 225.1±26.6 from 172.6±13.9, ALP 13.5±0.006 to 13.6±0.002, UREA 1.0±0.08 to 3.0±0.4, their reduction was observed in the extract-treated groups. ALT reduced from 96.1±22.4 to 73.70±9.7; AST from 225.1±26.6 to 184.4±18.2; ALP from 13.6±0.002 to 13.6±0.01; UREA from 3.0±0.4 to 2.0±0.4. Treatment with the extract significantly reduced markers of oxidative stress in the kidney [hydrogen peroxide (898.8±6.26 to 688.0±13.7), malondialdehyde (640±0.1 to 600±0.2) and protein carbonyl (548.4±1.5 to 458.1±1.6)]; heart [hydrogen peroxide (389.4±1.8 to 358.2±1.5), malondialdehyde (264.0±0.5 to 122.0±0.3), protein carbonyl (196.8±0.5 to 162.7±3.5)]; and liver [hydrogen peroxide (119.36±3.2 to 103.94±10.7), malondialdehyde (236.0±0.4 to 73.0±0.2), protein carbonyl (269.3±1.0 to 174.2±1.1) respectively. The levels of antioxidants were reduced in the diabetic untreated group but there was increase in the Moringa treated group. Glucose transporter 4 (GLUT 4) was down regulated in the diabetic untreated group while it was well expressed in the treated groups. The histology of pancreas and liver showed varied levels of infiltration of inflammatory cells, congestion and necrotic lesions, but these were mild in the treated groups. The result shows that the extract does have an anti-diabetic effect with the decrease in the levels of blood glucose and markers of oxidative stress as well as increase in the amount of antioxidants in the treated group when compared to the diabetic untreated group. More importantly, the extract caused upregulation of GLUT 4, which is relevant in reversing insulin resistance in the same manner as pioglitazone, the standard antidiabetic agent used in this study.

Bioanalytical Assay Development And Validation For The Pharmacokinetic Study Of Gmc1, A Novel Fkbp52 Co-Chaperone Inhibitor For Castration Resistant Prostate Cancer, Oscar Ekpenyong, Candace Cooper, Jing Ma, Naihsuan C. Guy, Ashley N. Payan, Fuqiang Ban, Artem Cherkasov, Marc B. Cox, Dong Liang, Huan Xie

Faculty Publications

Background: GMC1 (2-(1H-benzimidazol-2-ylsulfanyl)-N-[(Z)-(4-methoxyphenyl) methylideneamino] acetamide) effectively inhibits androgen receptor function by binding directly to FKBP52. This is a novel mechanism for the treatment of castration resistant prostate cancer (CRPC). Methods: an LC-MS/MS method was developed and validated to quantify GMC1 in plasma and urine from pharmacokinetics studies in rats. An ultra-high-performance liquid chromatography (UHPLC) system equipped with a Waters XTerra MS C18 column was used for chromatographic separation by gradient elution with 0.1% (v/v) formic acid in water and methanol. A Sciex 4000 QTRAP® mass spectrometer was used for analysis by multiple reaction monitoring (MRM) in positive mode; the specific …

Deficiency Of Splicing Factor 1 (Sf1) Reduces Intestinal Polyp Incidence In ApcMin/+ Mice, Jyotsna D. Godavarthi, Shahrazad Polk, Lisa Nunez, Amruthesh Shivachar, Nancy L. Glenn Griesinger, Angabin Matin

Faculty Publications

Background: Splicing factor 1 (SF1) is a conserved alternative splicing factor expressed in many different mammalian cell types. The genetically modified Sf1+/− (or Sf1β-geo/+ ) mice express reduced levels of SF1 protein in mouse tissues, including in cells of the intestines. Mutational inactivation of human adenomatous polyposis coli (APC) gene deregulates the Wnt signaling pathway and is a frequent genetic event in colon cancers. Mice with a point mutation in the Apc gene (ApcMin/+ ) also develop numerous intestinal polyps at a young age. Our aim was to determine the effect of reduced SF1 levels on polyp development due to …

Trends And Associated Characteristics For Chagas Disease Among Women Of Reproductive Age In The United States, 2002 To 2017, Chioma Ikedionwu, Deepa Dongarwar, Manvir Kaur, Lisa Nunez, Annabella Awazi, Jere' Mallet, Ka Shena Kennedy, Michelle Cano, Chinwe Dike, Jessica Okwudi, Justice Stewart, David Igwegbe, Flora G. Estes, Kiara K. Spooner, Jason L. Salemi, Hamisu M. Salihu, Omonike A. Olaleye

Faculty Publications

Background: American trypanosomiasis, commonly referred to as Chagas disease, is caused by a single cell protozoan known as Trypanosoma cruzi (T. cruzi). Although those affected are mainly in Latin America, Chagas has been detected in the United States (US), Canada and in many European countries due to migration. Few studies have explored the epidemiology of Chagas within the US or changes in disease burden over the past decade. The objective of this study was to explore the trends and associated characteristics for Chagas disease among hospitalized women of reproductive age in the US. Methods: We analyzed admissions data including socio-demographic …

Histone Deacetylase Inhibitors As Multitarget-Directed Epi-Drugs In Blocking Pi3k Oncogenic Signaling: A Polypharmacology Approach, Kasturi Ranganna, Chelliah Selvam, Amruthesh Shivachar, Zivar Yousefipour

Faculty Publications

Genetic mutations and aberrant epigenetic alterations are the triggers for carcinogenesis. The emergence of the drugs targeting epigenetic aberrations has provided a better outlook for cancer treatment. Histone deacetylases (HDACs) are epigenetic modifiers playing critical roles in numerous key biological functions. Inappropriate expression of HDACs and dysregulation of PI3K signaling pathway are common aberrations observed in human diseases, particularly in cancers. Histone deacetylase inhibitors (HDACIs) are a class of epigenetic small-molecular therapeutics exhibiting promising applications in the treatment of hematological and solid malignancies, and in non-neoplastic diseases. Although HDACIs as single agents exhibit synergy by inhibiting HDAC and the PI3K …

Temporal Trends Of Gestational Malaria In The United States, Justin Alexander, Deepa Dongarwar, Emmanuella Oduguwa, Larianna Varnado, Adesola Adenote, Jade Bailey, Chibueze Ezeudu, Patrice Nelson, Alexis Shavers, Abimbola Telufusi, Kiara K. Spooner, Jason L. Salemi, Omonike A. Olaleye, Hamisu M. Salihu

Faculty Publications

Background: Although regarded as rare in the United States (US), increased global traffic and importation of malaria from endemic countries may lead to a rise in gestational malaria in the US. Methods: This multi-year retrospective study analyzed trends in diagnosed cases of gestational malaria from 2002 to 2017 using joinpoint regression models. We also assessed the association between gestational malaria and selected maternal-fetal adverse outcomes. Results: Mothers diagnosed with gestational malaria tended to be older, and the majority of diagnosed cases (52.9%) were among Non-Hispanic (NH) Blacks. Diagnosed cases of gestational malaria are on the rise in the US. Mothers …

Analysis Of Factors Contributing To The Severity Of Large Truck Crashes, Jinhong Li, Jinli Liu, Pengfei Liu, Yi Qi

Faculty Publications

Crashes that involved large trucks often result in immense human, economic, and social losses. To prevent and mitigate severe large truck crashes, factors contributing to the severity of these crashes need to be identified before appropriate countermeasures can be explored. In this research, we applied three tree‐based machine learning (ML) techniques, i.e., random forest (RF), gradient boost decision tree (GBDT), and adaptive boosting (AdaBoost), to analyze the factors contributing to the severity of large truck crashes. Besides, a mixed logit model was developed as a baseline model to compare with the factors identified by the ML models. The analysis was …

National Income Inequality And International Business Expansion, Nathaniel C. Lupton, Guoliang Frank Jiang, Luis F. Escobar, Alfredo Jiménez

Faculty Publications

We examine the extent to which host country income inequality influences multinational enterprises’ (MNE) expansion strategy for foreign production investment, depending on their specific strategic objectives. Applying a transaction cost framework, we predict that national income inequality has an inverted U-shaped relationship with foreign production investment. As inequality increases, MNEs accrue lower transaction costs arising from interactions with various local actors, leading to higher probability of investment. As income inequality increases further, its effect on location attractiveness will become negative, as its attraction effect is increasingly offset by additional monitoring, bargaining, and security costs owing to the more fractious nature …

Impact Of Mass Distribution Of Long-Lasting Insecticidal Nets (Llins) In Mozambique, 2011 To 2025: Retrospective And Prospective Modelling Of Child Mortality And Lives Saved, Erica Wetzler, Jorge A. H. Arroz, Chulwoo Park, Marta Chande, Figueiredo Mussambala

Faculty Publications

No abstract provided.

Genome‐Wide Association Study For Host Genetic Factors Associated With Equine Herpesvirus Type‐1 Induced Myeloencephalopathy, Wangisa M. B. Dunuwille, Navid Yousefimashouf, Udeni B. R. Balasuriya, Nicola Pusterla, Ernest Bailey

Faculty Publications

No abstract provided.

Mud-Associated Organic Matter And Its Direct And Indirect Role In Marsh Organic Matter Accumulation And Vertical Accretion, G. Mariotti, T. Elsey-Quirk, G. Bruno, K. Valentine

Faculty Publications

In situ plant production is often assumed to be the major contributor to organic matter (OM) accumulation and vertical accretion in tidal marshes. Here, we evaluate the contribution of mud-associated OM in salt and brackish marshes in Louisiana. Based on 14 soil cores, the OM content of the mud fraction—i.e., any material smaller than 64 μm—was 17% ± 7% for the salt marshes and 28% ± 14% for the brackish marshes. This remains nearly uniform over the top 35 cm depth, suggesting that this material is deposited contemporaneously with the mud. The dry bulk density of the mud (300–450 kg …

The Carbon Stock And Sequestration Rate In Tidal Flats From Coastal China, Jie Chen, Dongqi Wang, Yangjie Li, Zhongjie Yu, Shu Chen, Xiyong Hou, John R. White, Zhenlou Chen

Faculty Publications

Tidal flats form around the estuarine and coastal zone by continuous terrigenous sediment transport and deposition processes. Now a large body of published carbon research work frame within the vegetated area (mangrove forests, sea grass bed, and salt marshes). Nonvegetated tidal flats, which are characterized by predominantly silts and clays sediment, were generally impressed with low carbon stock due to their meager primary productivity. However, these regions may be a potentially important carbon sink, given their high burial rate, expanding areal coverage, and detrial organic carbon derived from watershed and adjacent vegetated area. Low carbon densities (< 0.01 g cm⁻³) were …

Atmospheric Cold Front-Generated Waves In The Coastal Louisiana, Yuhan Cao, Chunyan Li, Changming Dong

Faculty Publications

Atmospheric cold front-generated waves play an important role in the air–sea interaction and coastal water and sediment transports. In-situ observations from two offshore stations are used to investigate variations of directional waves in the coastal Louisiana. Hourly time series of significant wave height and peak wave period are examined for data from 2004, except for the summer time between May and August, when cold fronts are infrequent and weak. The intra-seasonal scale variations in the wavefield are significantly affected by the atmospheric cold frontal events. The wave fields and directional wave spectra induced by four selected cold front passages over …

The Impacts Of Building Characteristics, Social Psychological And Cultural Factors On Indoor Environment Quality Productivity Belief, Chien Fei Chen, Selin Yilmaz, Anna Laura Pisello, Marilena De Simone, Amy Kim, Tianzhen Hong, Karol Bandurski, Mateus V. Bavaresco, Pei Ling Liu, Yimin Zhu

Faculty Publications

Indoor environmental quality (IEQ) plays a key role in determining occupants' productivity at work; however, analyses of the interconnected factors among building physical, attitudinal, social and demographic components in one study are lacking. To fill this research gap, this study investigates these interconnected factors' influence on occupants’ IEQ-productivity belief, defined as a personal, subjective evaluation of the linkage between the impacts of five IEQ aspects (the quality of indoor temperature, air, natural and electric lighting, and acoustics) and productivity. A cross-sectional survey data was collected in university offices from six countries (Brazil, Italy, Poland, Switzerland, Taiwan and the U.S.). Results …

Scaling Invisible Walls: Reasserting Indigenous Persistence In Mission-Era California, Tsim D. Schneider, Khal Schneider, Lee M. Panich

Faculty Publications

California’s Franciscan missions were grounded in Indigenous homelands that to this day remain largely undertheorized and trivialized by scholarly and popular understandings of missions as inescapable fortresses of confinement. Narratives that position California’s missions as places of Indigenous imprisonment endure but they are at odds with a growing body of archaeological and documentary evidence demonstrating the persistence of Native lives, activities, and decision-making taking place within and beyond the walls of missions. We argue that interpretations of the missions in scholarly and popular conversation must make Indigenous persistence and resilient relationships to meaningful landscapes the cardinal priorities, not secondary attributes, …

Review Of Black Buck, Michael F. Russo

Faculty Publications

No abstract provided.

Chain-Selective Isotopic Labeling Of The Heterodimeric Type Iii Secretion Chaperone, Scc4:Scc1, Reveals The Total Structural Rearrangement Of The Chlamydia Trachomatis Bi-Functional Protein, Scc4, Thilini O. Ukwaththage, Samantha M. Keane, Li Shen, Megan A. Macnaughtan

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Scc4 is an unusual bi-functional protein from Chlamydia trachomatis (CT) that functions as a type III secretion system (T3SS) chaperone and an RNA polymerase (RNAP)-binding protein. Both functions require interactions with protein partners during specific stages of the CT developmental cycle. As a T3SS chaperone, Scc4 binds Scc1 during the late stage of development to form a heterodimer complex, which chaperones the essential virulence effector, CopN. During the early-middle stage of development, Scc4 regulates T3SS gene expression by binding the σ66-containing RNAP holoenzyme. In order to study the structure and association …

Community Context And Dispersal Stochasticity Drive Variation In Spatial Spread, Tad Dallas, Brett Melbourne, Alan Hastings

Faculty Publications

© 2020 British Ecological Society Dispersal is a key process in shaping species spatial distributions. Species interactions and variation in dispersal probabilities may jointly influence species spatial dynamics. However, many studies examine dispersal as a neutral process, independent of community context or intraspecific variation in dispersal behaviour. Here, we use controlled, replicated communities of two Tribolium species (T. castaneum and T. confusum) to examine how intraspecific variation in dispersal behaviour and community context influence dispersal dynamics in simple experimental landscapes composed of homogeneous habitat patches. We found considerable individual-level variation in dispersal probability that was unrelated to body size variation. …

Early Intracellular Trafficking And Subsequent Activity Of Programmed Cell Death In Channel Catfish Macrophages Infected With Edwardsiella Ictaluri, Lidiya P. Dubytska, Ronald L. Thune

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. The development of Edwardsiella-containing-vacuoles (ECV) and the ability of Edwardsiella ictaluri to survive and replicate within macrophages suggests a unique process relative to normal phagosomal/lysosomal maturation and programed cell death. Developing ECV showed that endosomal membrane markers Rab5, EEA1, and Rab7 were all detected in both the wild type (WT) and an E. ictaluri type-3 secretion system (T3SS) mutant, 65ST. Co-localization with Lamp1, however, was significantly lower in the WT. The host cell endoplasmic reticulum marker, calnexin, co-localized to 65ST ECV significantly more than WT ECV, while Golgi vesicle marker, giantin, …

Psychological, Addictive, And Health Behavior Implications Of The Covid-19 Pandemic, Michael J. Zvolensky, Lorra Garey, Andrew H. Rogers, Norman B. Schmidt, Anka .. Vujanovic, Eric A. Storch, Julia D. Buckner, Daniel J. Paulus, Candice Alfano, Jasper A. J. Smits, Connall O'Cleirigh

Faculty Publications

No abstract provided.

Scale(S) Matter: Deconstructing An Area Of Endemism For Middle American Freshwater Fishes, Diego J. Elías, Caleb D. Mcmahan, Wilfredo A. Matamoros, Adan E. Gómez-González, Kyle R. Piller, Prosanta Chakrabarty

Faculty Publications

© 2020 John Wiley & Sons Ltd Aim: The processes that generate biodiversity occur at finer scales than are often studied, particularly in freshwater systems of the northern Neotropics. We investigate whether fine-scale biogeographic patterns are present within the larger Grijalva–Usumacinta Area of Endemism (AoE) —a region in Middle America where nearly 60% of freshwater fishes are endemic—and if present-day river basins are single historical units. Location: Northern Central America and southern Mexico. Taxon: Freshwater fishes (e.g., Cichlidae, Poeciliidae, Ostariophysi). Methods: We used fine-scale distributional data for freshwater fishes in northern Middle America (based on museum records and fieldwork) and …

Microbiological, Physicochemical, And Immunological Analysis Of A Commercial Cashew Nut-Based Yogurt, Christopher P. Mattison, Kayanush Aryana, Kristen Clermont, Eric Prestenburg, Steven W. Lloyd, Casey .. Grimm, Richard L. Wasserman

Faculty Publications

Nut-based milks and yogurts are gaining popularity, but may not offer the same benefits as dairy yogurts to consumers. Cashew nuts often cause severe allergic reactions, and cashew nut allergens are stable to several types of processing. To compare its characteristics to dairy yogurt and characterize the effects of fermentation on the Ana o 1-3 cashew nut allergens, a commercial yogurt made from cashew nuts (Cashewgurt) was evaluated for microbiological, physiochemical, and immunological properties. Average counts for lactobacilli and Streptococcus thermophilus were greater than 10 million colony forming units per milliliter, indicating the capacity to provide a health benefit. Cashewgurt …

A Novel Mutation Of The Narrow Leaf 1 Gene Adversely Affects Plant Architecture In Rice (Oryza Sativa L.), Prasanta K. Subudhi, Richard S. Garcia, Sapphire Coronejo, Teresa B. De Leon

Faculty Publications

Plant architecture is critical for enhancing the adaptability and productivity of crop plants. Mutants with an altered plant architecture allow researchers to elucidate the genetic network and the underlying mechanisms. In this study, we characterized a novel nal1 rice mutant with short height, small panicle, and narrow and thick deep green leaves that was identified from a cross between a rice cultivar and a weedy rice accession. Bulked segregant analysis coupled with genome re-sequencing and cosegregation analysis revealed that the overall mutant phenotype was caused by a 1395-bp deletion spanning over the last two exons including the transcriptional end site …

Speciation-By-Depth On Coral Reefs: Sympatric Divergence With Gene Flow Or Cryptic Transient Isolation?, Carlos Prada, Michael E. Hellberg

Faculty Publications

The distributions of many sister species in the sea overlap geographically but are partitioned along depth gradients. The genetic changes leading to depth segregation may evolve in geographic isolation as a prerequisite to coexistence or may emerge during primary divergence leading to new species. These alternatives can now be distinguished via the power endowed by the thousands of scorable loci provided by second-generation sequence data. Here, we revisit the case of two depth-segregated, genetically isolated ecotypes of the nominal Caribbean candelabrum coral Eunicea flexuosa. Previous analyses based on a handful of markers could not distinguish between models of genetic exchange …

Mere Common Ownership And The Antitrust Laws, Thomas A. Lambert

Faculty Publications

"Common ownership," also called "horizontal shareholding," refers to a stock investor's ownership of minority stakes in multiple competing firms. Recent empirical studies have purported to show that institutional investors' common ownership reduces competition among commonly owned competitors. "Mere common ownership" is horizontal shareholding that is not accompanied by any sort of illicit agreement, such as a hub-and-spoke conspiracy, or the holding of a control-conferring stake. This Article considers the legality of mere common ownership under the U.S. antitrust laws. Prominent antitrust scholars and the leading treatise have concluded that mere common ownership that has the incidental effect of lessening market …

Amicus Curiae Briefs: A Message From The 7th Circuit, Douglas E. Abrams

Faculty Publications

Like other brief writers, the amicus brief’s writer must heed the court’s rules of practice and procedure, including rules that prescribe a brief’s maximum page length. But a brief writer can meet the court’s circumstances and expectations without going to the max. A few months before he ascended to the Supreme Court bench in 1943, D.C. Circuit Judge Wiley B. Rutledge advised advocates to strike a balance by being “as brief as one can be consistent with adequate and clear presentation of his case."

An amicus’ prudent approach to concise brief writing is to adapt the advice delivered by opera …

Mutations In The Pectin Methyltransferase Quasimodo2 Influence Cellulose Biosynthesis And Wall Integrity In Arabidopsis, Juan Du, Alex Kirui, Shixin Huang, Lianglei Wang, William J. Barnes, Sarah N. Kiemle, Yunzhen Zheng, Yue Rui, Mei Ruan, Shiqian Qi, Seong H. Kim, Tuo Wang, Daniel J. Cosgrove, Charles T. Anderson, Chaowen Xiao

Faculty Publications

© 2020 ASPB. Pectins are abundant in the cell walls of dicotyledonous plants, but how they interact with other wall polymers and influence wall integrity and cell growth has remained mysterious. Here, we verified that QUASIMODO2 (QUA2) is a pectin methyltransferase and determined that QUA2 is required for normal pectin biosynthesis. To gain further insight into how pectin affects wall assembly and integrity maintenance, we investigated cellulose biosynthesis, cellulose organization, cortical microtubules, and wall integrity signaling in two mutant alleles of Arabidopsis (Arabidopsis thaliana) QUA2, qua2 and tsd2. In both mutants, crystalline cellulose content is reduced, cellulose synthase particles move …

A Theory Of Multiformat Communication: Mechanisms, Dynamics, And Strategies, Jordan W. Moffett, Judith Anne Garretson Folse, Robert W. Palmatier

Faculty Publications

Extant communication theories predate the explosion of digital formats and technological advances such as virtual reality, which likely explains their predominant focus on traditional and format-level (e.g., face-to-face, email) rather than digital or characteristic-level (e.g., visual cues, synchronicity) design decisions. Firms thus lack insights into how to create and use emerging digital formats, individually or synergistically. To establish a holistic framework of bilateral multiformat communication for relationship marketing, this article reviews communication theory to establish a foundation for understanding multiformat communication and to identify any gaps (e.g., AI agents, simulated cues). The authors then review bilateral communication research in light …

A Coded Aperture Microscope For X-Ray Fluorescence Full-Field Imaging, D. P. Siddons, A. J. Kuczewski, A. K. Rumaiz, R. Tappero, M Idir, K. Nakhoda, J. Khanfri, V. Singh, E. R. Farquhar, M. Sullivan, D. Abel, D. J. Brady, X. Yuan

Faculty Publications

The design and construction of an instrument for full-field imaging of the X-ray fluorescence emitted by a fully illuminated sample are presented. The aim is to produce an X-ray microscope with a few micrometers spatial resolution, which does not need to scan the sample. Since the fluorescence from a spatially inhomogeneous sample may contain many fluorescence lines, the optic which will provide the magnification of the emissions must be achromatic, i.e. its optical properties must be energy-independent. The only optics which fulfill this requirement in the X-ray regime are mirrors and pinholes. The throughput of a simple pinhole is very …