Digital Commons Network^™

Ferric Uptake Regulator (Fur) Reversibly Binds A [2fe-2s] Cluster To Sense Intracellular Iron Homeostasis In Escherichia Coli, Chelsey R. Fontenot, Homyra Tasnim, Kathryn A. Valdes, Codrina V. Popescu, Huangen Ding

Faculty Publications

© 2020 Fontenot et al. The ferric uptake regulator (Fur) is a global transcription factor that regulates intracellular iron homeostasis in bacteria. The current hypothesis states that when the intracellular “free” iron concentration is elevated, Fur binds ferrous iron, and the iron-bound Fur represses the genes encoding for iron uptake systems and stimulates the genes encoding for iron storage proteins. However, the “iron-bound” Fur has never been isolated from any bacteria. Here we report that the Escherichia coli Fur has a bright red color when expressed in E. coli mutant cells containing an elevated intracellular free iron content because of …

The Transition From Stochastic To Deterministic Bacterial Community Assembly During Permafrost Thaw Succession, Stacey J. Doherty, Robyn A. Barbato, A. Stuart Grandy, W. Kelley Thomas, Sylvain Monteux, Ellen Dorrepaal, Margareta Johansson, Jessica G. Ernakovich

Faculty Publications

The Northern high latitudes are warming twice as fast as the global average, and permafrost has become vulnerable to thaw. Changes to the environment during thaw leads to shifts in microbial communities and their associated functions, such as greenhouse gas emissions. Understanding the ecological processes that structure the identity and abundance (i.e., assembly) of pre- and post-thaw communities may improve predictions of the functional outcomes of permafrost thaw. We characterized microbial community assembly during permafrost thaw using in situ observations and a laboratory incubation of soils from the Storflaket Mire in Abisko, Sweden, where permafrost thaw has occurred over the …

N,S-Co-Doped Carbon Dots For Rapid Acid Test Paper And Bioimaging, Juan Hou, Jing Qin, Hongyu Pang, Xu Gao, Tiedong Sun, Bin Li

Faculty Publications

Fluorescent N,S-CDs with a quantum yield of 37.8% were synthesized via a one-pot solvothermal method. Detailed characterizations on physical, chemical and optical properties have been investigated. N,S-CDs demonstrated remarkably quenched and enhanced fluorescence in acidic and basic media. Direct qualitative analysis in pH sensor and cell imaging were preliminarily studied. This journal is

Dense Sampling Of Bird Diversity Increases Power Of Comparative Genomics, Shaohong Feng, Josefin Stiller, Yuan Deng, Joel Armstrong, Qi Fang, Andrew Hart Reeve, Duo Xie, Guangji Chen, Chunxue Guo, Brant C. Faircloth, Bent Petersen, Zongji Wang, Qi Zhou, Mark Diekhans, Wanjun Chen, Sergio Andreu-Sánchez, Ashot Margaryan, Jason Travis Howard, Carole Parent, George Pacheco, Mikkel Holger S. Sinding, Lara Puetz, Emily Cavill, Ângela M. Ribeiro, Leopold Eckhart, Jon Fjeldså, Peter A. Hosner, Robb T. Brumfield, Les Christidis, Mads F. Bertelsen, Thomas Sicheritz-Ponten, Dieter Thomas Tietze, Bruce C. Robertson, Gang Song

Faculty Publications

© 2020, The Author(s). Whole-genome sequencing projects are increasingly populating the tree of life and characterizing biodiversity1–4. Sparse taxon sampling has previously been proposed to confound phylogenetic inference5, and captures only a fraction of the genomic diversity. Here we report a substantial step towards the dense representation of avian phylogenetic and molecular diversity, by analysing 363 genomes from 92.4% of bird families—including 267 newly sequenced genomes produced for phase II of the Bird 10,000 Genomes (B10K) Project. We use this comparative genome dataset in combination with a pipeline that leverages a reference-free whole-genome alignment to identify orthologous regions in greater …

Inferring Life History Characteristics Of The Oceanic Whitetip Shark Carcharhinus Longimanus From Vertebral Bomb Radiocarbon, Michelle S. Passerotti, Allen H. Andrews, Lisa J. Natanson

Faculty Publications

Oceanic whitetip sharks Carcharhinus longimanus are a cosmopolitan epipelagic species that was once prolific throughout the tropics and subtropics but was recently listed as Critically Endangered by the International Union for the Conservation of Nature and as Threatened under the United States Endangered Species Act. Although historically conspicuous in oceanic fisheries catches, relatively little is known about their habitat use, movement, and life history during migration. Given the paucity of data on migratory patterns and lack of age estimate validation available for this species, we evaluated vertebral growth bands for bomb radiocarbon (14C) patterns to derive additional information on these …

Mapping Flat, Deep, And Slow: On The 'Spirit Of Place' In New Cinema History, Jeffrey Klenotic

Faculty Publications

This essay engages in a creative, heuristic, and reflexive consideration of the ‘localities’ of cinema audiences by exploring New Cinema History as a place. New Cinema History is conceptualised as a place continually produced in and through its interactions with the heterogeneous multiplicities of situated audiences and experiences of cinema that form the topoi of its landscape of inquiry. In reflecting on how this placialised landscape has been and might be represented, I argue that New Cinema History’s ‘spirit of place’ is most productive when rendered within a ‘splatial’ framework that draws upon practices of flat, deep, and slow mapping …

White Paper: From Bound States To The Continuum, Calvin W. Johnson, Kristina D. Launey, Naftali Auerbach, Sonia Bacca, Bruce R. Barrett, Carl R. Brune, Mark A. Caprio, Pierre Descouvemont, W. H. Dickhoff, Charlotte Elster, Patrick J. Fasano, Kevin Fossez, Heiko Hergert, Morten Hjorth-Jensen, Linda Hlophe, Baishan Hu, Rodolfo M. Id Betan, Andrea Idini, Sebastian König, Konstantinos Kravvaris, Dean Lee, Jin Lei, Alexis Mercenne, Rodrigo Navarro Perez, Witold Nazarewicz, Filomena M. Nunes, Marek Płoszajczak, Jimmy Rotureau, Gautam Rupak, Andrey M. Shirokov, Ian Thompson, James P. Vary, Alexander Volya

Faculty Publications

This white paper reports on the discussions of the 2018 Facility for Rare Isotope Beams Theory Alliance (FRIB-TA) topical program ‘From bound states to the continuum: Connecting bound state calculations with scattering and reaction theory’. One of the biggest and most important frontiers in nuclear theory today is to construct better and stronger bridges between bound state calculations and calculations in the continuum, especially scattering and reaction theory, as well as teasing out the influence of the continuum on states near threshold. This is particularly challenging as many-body structure calculations typically use a bound state basis, while reaction calculations more …

Development Of A Comprehensive Measure Of Organizational Readiness (Motivation × Capacity) For Implementation: A Study Protocol, Timothy J. Walker, Heather M. Brandt, Abraham Wandersman, Jonathan Scaccia, Andrea Lamont, Lauren Workman, Emanuelle Dias, Pamela M. Diamond, Derek W. Craig, Maria E. Fernandez

Faculty Publications

BACKGROUND: Organizational readiness is important for the implementation of evidence-based interventions. Currently, there is a critical need for a comprehensive, valid, reliable, and pragmatic measure of organizational readiness that can be used throughout the implementation process. This study aims to develop a readiness measure that can be used to support implementation in two critical public health settings: federally qualified health centers (FQHCs) and schools. The measure is informed by the Interactive Systems Framework for Dissemination and Implementation and R = MC heuristic (readiness = motivation × innovation-specific capacity × general capacity). The study aims are to adapt and further develop …

Short-Time Dynamics Of Pdms- G-Pdms Bottlebrush Polymer Melts Investigated By Quasi-Elastic Neutron Scattering, Karin J. Bichler, Bruno Jakobi, Victoria García Sakai, Alice Klapproth, Richard A. Mole, Gerald J. Schneider

Faculty Publications

© We have studied the short-time dynamical behavior of polydimethylsiloxane (PDMS) bottlebrush polymers, PDMS-g-PDMS. The samples have similar backbone lengths but different side-chain lengths, resulting in a shape transition. Quasi-elastic neutron scattering was used to observe the dynamical changes inherent to these structural changes. The combination of data from three spectrometers enabled to follow the dynamics over broad frequency and temperature ranges, which included segmental relaxations and more localized motions. The latter, identified as the methyl group rotation, is described by a threefold jump model and shows higher activation energies compared to linear PDMS. The segmental relaxation times, τs, decrease …

Interplay Of Changing Irrigation Technologies And Water Reuse: Example From The Upper Snake River Basin, Idaho, Usa, Shan Zuidema, Danielle S. Grogan, Alexander Prusevich, Richard B. Lammers, Sarah Gilmore, Paula Williams

Faculty Publications

Careful allotment of water resources for irrigation is critical for ensuring the resiliency of agriculture in semiarid regions, and modernizing irrigation technology to minimize inefficient water losses is an important tool for farmers and agricultural economies. While modernizing irrigation technology can achieve reductions in the nonbeneficial use of water, such as bare soil evaporation and nonconsumptive losses, water returned to the landscape is also reduced, often eliminating flow paths that other users rely on. In basins using a combination of surface and groundwater, replenishing aquifer storage by the managed aquifer recharge (MAR) of seasonally available water can mitigate the aquifer …

A Cross-Country And Country-Specific Modelling Of Stock Market Performance, Bank Development And Global Equity Index In Emerging Market Economies: A Case Of Brics Countries, Ebere Ume Kalu, Augustine C. Arize

Faculty Publications

This study investigated in cross-country and panel form the interactions of bank development, stock market development and global equity index, focusing on the BRICS countries covering the period 1990 to 2018. We found a bidirectional causation between bank development (CPSGDP) and stock market performance as proxied by the depth of the markets (MCAPGDP) in the BRICS countries. Cointegration was also found using the panel cointegration framework and the bounds test for the ARDL estimators. This largely proves that a long-run relationship of both direct and reverse nature exists between bank development and stock market performance. For the bank development and …

Facets Of Christian Leadership, Petr Cincala

Faculty Publications

No abstract provided.

Two Kinds Of Polar Knowledge, Lawrence C. Hamilton

Faculty Publications

Outreach and communication with the public have substantial value in polar research, in which studies often find changes of global importance that are happening far out of sight from the majority of people living at lower latitudes. Seeking evidence on the effectiveness of outreach programs, the U.S. National Science Foundation sponsored large-scale survey assessments before and after the International Polar Year in 2007/2008. Polar-knowledge questions have subsequently been tested and refined through other nationwide and regional surveys. More than a decade of such work has established that basic but fairly specific knowledge questions, with all answer choices sounding plausible but …

Neutrino Interaction Classification With A Convolutional Neural Network In The Dune Far Detector, B. Abi, R. Acciarri, M. A. Acero, G. Adamov, D. Adams, M. Adinolfi, Z. Ahmad, J. Ahmed, T. Alion, S. Alonso Monsalve, C. Alt, J. Anderson, C. Andreopoulos, M. P. Andrews, F. Andrianala, S. Andringa, A. Ankowski, M. Antonova, S. Antusch, A. Aranda-Fernandez, A. Ariga, L. O. Arnold, M. A. Arroyave, J. Asaadi, A. Aurisano, V. Aushev, D. Autiero, F. Azfar

Faculty Publications

The Deep Underground Neutrino Experiment is a next-generation neutrino oscillation experiment that aims to measure CP-violation in the neutrino sector as part of a wider physics program. A deep learning approach based on a convolutional neural network has been developed to provide highly efficient and pure selections of electron neutrino and muon neutrino charged-current interactions. The electron neutrino (antineutrino) selection efficiency peaks at 90% (94%) and exceeds 85% (90%) for reconstructed neutrino energies between 2-5 GeV. The muon neutrino (antineutrino) event selection is found to have a maximum efficiency of 96% (97%) and exceeds 90% (95%) efficiency for reconstructed neutrino …

Neutrino Interaction Classification With A Convolutional Neural Network In The Dune Far Detector, B. Abi, R. Acciarri, M. A. Acero, G. Adamov, D. Adams, M. Adinolfi, Z. Ahmad, J. Ahmed, T. Alion, S. Alonso Monsalve, C. Alt, J.. Anderson, C. Andreopoulos, M. P. Andrews, F. Andrianala, S. Andringa, A. Ankowski, M. Antonova, S. Antusch, A. Aranda-Fernadez, Roberto Petti, Et. Al.

Faculty Publications

The Deep Underground Neutrino Experiment is a next-generation neutrino oscillation experiment that aims to measure CP-violation in the neutrino sector as part of a wider physics program. A deep learning approach based on a convolutional neural network has been developed to provide highly efficient and pure selections of electron neutrino and muon neutrino charged-current interactions. The electron neutrino (antineutrino) selection efficiency peaks at 90% (94%) and exceeds 85% (90%) for reconstructed neutrino energies between 2–5 GeV. The muon neutrino (antineutrino) event selection is found to have a maximum efficiency of 96% (97%) and exceeds 90% (95%) efficiency for reconstructed …

Civilian Pepper Spray For Self Defense: Understanding User Perception And Impact Of Design On User Performance, David Strybel, Anil R. Kumar

Faculty Publications

Pepper spray is widely used in the US, and is marketed as an effective self-defense device. While pepper spray can be useful in deterring an attacker, many pepper spray owners do not have any experience using it. There is a dearth of published studies that focus on civilian pepper-spray use, not to mention first time pepper-spray users. A study to analyze pepper-spray designs with first-time users, to see how the design, specifically the safety mechanism, affects response time and overall performance would be helpful. The study was conducted in 2 parts – a survey to understand user perception, and a …

Fungal Community Structure And Function Shifts With Atmospheric Nitrogen Deposition, Jessica A. M. Moore, Mark A. Anthony, Gregory J. Pec, Lidia K. Trocha, Artur Trzebny, Kevin M. Geyer, Linda T. A. Van Diepen, Serita D. Frey

Faculty Publications

Fungal decomposition of soil organic matter depends on soil nitrogen (N) availability. This ecosystem process is being jeopardized by changes in N inputs that have resulted from a tripling of atmospheric N deposition in the last century. Soil fungi are impacted by atmospheric N deposition due to higher N availability, as soils are acidified, or as micronutrients become increasingly limiting. Fungal communities that persist with chronic N deposition may be enriched with traits that enable them to tolerate environmental stress, which may trade-off with traits enabling organic matter decomposition. We hypothesized that fungal communities would respond to N deposition by …

¿Existe Un Español Antillano?, Orlando Alba

Faculty Publications

El objetivo fundamental de esta exposición consiste en demostrar, con el apoyo de más de 80 audio-clips y desde una perspectiva sociolingüística, que, a pesar de las conocidas semejanzas existentes en su modo de hablar, Cuba, República Dominicana y Puerto Rico mantienen su propia identidad lingüística.

Cada isla manifiesta su propia idiosincrasia lingüística, tanto en el terreno objetivo, de la actuación, como en el subjetivo, de la percepción. En el plano objetivo, existen estructuras sintácticas y unidades léxicas que actúan como elementos distintivos. Asimismo, son notables las discrepancias fonéticas cuantitativas y cualitativas entre los dialectos de cada país. A esto …

«Astrología Y Genealogía De Poder En La Vida Es Sueño De Calderón De La Barca Y La Comedia Anónima El Vaticinio Cumplido: La Estrella De Inglaterra.», Carmela V. Mattza

Faculty Publications

¿En qué medida la relación entre astrología y genealogía de autoridad y poder presente en la comedia calderoniana puede servirnos para estudiar otras comedias áureas de temas parecidos? ¿Hasta qué punto la presencia de esos discursos genealógicos puede echar luz sobre el uso de la comedia como propaganda? A manera de respuesta, en la presente comunicación presentada en el congreso de la Asociación Internacional de Siglo de Oro celebrado en Madrid en 2017, me propongo estudiar y comparar la función del eclipse en La vida es sueño, la comedia paradigmática de Calderón, con la que aparece en la comedia …

Selective One-Dimensional 13C-13C Spin-Diffusion Solid-State Nuclear Magnetic Resonance Methods To Probe Spatial Arrangements In Biopolymers Including Plant Cell Walls, Peptides, And Spider Silk, Bennett Addison, Dillan Stengel, Vivek S. Bharadwaj, Renee M. Happs, Crissa Doeppke, Tuo Wang, Yannick J. Bomble, Gregory P. Holland, Anne E. Harman-Ware

Faculty Publications

© 2020 American Chemical Society. All rights reserved. Two-dimensional (2D) and 3D through-space 13C-13C homonuclear spin-diffusion techniques are powerful solid-state nuclear magnetic resonance (NMR) tools for extracting structural information from 13C-enriched biomolecules, but necessarily long acquisition times restrict their applications. In this work, we explore the broad utility and underutilized power of a chemical shift-selective one-dimensional (1D) version of a 2D 13C-13C spin-diffusion solid-state NMR technique. The method, which is called 1D dipolar-assisted rotational resonance (DARR) difference, is applied to a variety of biomaterials including lignocellulosic plant cell walls, microcrystalline peptide fMLF, and black widow dragline spider silk. 1D 13C-13C …

Collegiate Student-Athletes' Perceptions Of Patient-Centered Care Delivered By Athletic Trainers, Ansley Smallen Redinger, Zachary K. Winkelmann Phd, Lat, Atc, Lindsey E. Eberman

Faculty Publications

Context

The health care core competencies indicate that all medical professionals should provide patient-centered care (PCC), which is defined as care that is respectful and responsive to the patient's values and preferences, during each encounter. Objective

To identify collegiate student-athletes' definitions of PCC and measure their perceived level of PCC from an athletic trainer (AT). Design

Cross-sectional study. Setting

Mixed-methods survey. Patients or Other Participants

A total of 610 (age = 19 ± 1 year) National Collegiate Athletic Association student-athletes completed the survey. Main Outcome Measure(s)

The survey consisted of 1 open-ended question that prompted the participant to define PCC …

Classification Of Planetary Craters Using Outline-Based Morphometrics, Thomas J. Slezak, Jani Radebaugh, Eric H. Christiansen, Mark C. Belk

Faculty Publications

The morphologies of craters on planetary surfaces reveal clues about the geologic mechanisms by which they originate and subsequently evolve, as well as the materials and physical variables inherent to the environment in which they formed. We carried out a quantitative multivariate analysis of shape descriptors derived from the outlines of craters formed by volcanic processes on Mars, Io, and Earth and by impact cratering on the Moon using elliptic Fourier analysis (EFA) and the Zahn-Roskies (Z-R) shape function. Canonical variate analysis (CVA) was used to construct a statistical model of differences between the crater groups to classify …

Social Contact, Time Alone, And Parental Subjective Well-Being: A Focus On Stay-At-Home Fathers Using The American Time Use Survey, Erin K. Holmes, Jocelyn Wikle, Clare R. Thomas, Mckell A. Jorgensen, Braquel R. Egginton

Faculty Publications

Stay-at-home fathers (SAHFs) face negative stereotypes and social stigma, which may be linked to negative feelings during social contact. In this study, we compare SAHFs' social contact and time alone to that of stay-at-home mothers and parents of other work/caregiving statuses. In addition, we analyze SAHFs' subjective well-being when with their children, spouse, noon spouse adults, and when alone to more accurately capture the positive and negative valences of their experiences. Using individual-level time-use diaries form the American Time Use Survey (N = 35, 959), a nationally representative sample, we find that compared to fathers working full time, SAHFs …

Self-Reported Health Without Clinically Measurable Benefits Among Adult Users Of Multivitamin And Multimineral Supplements: A Cross-Sectional Study, Manish D. Paranjpe, Alfred C. Chin, Ishan Paranjpe, Nicholas J. Reid, Phan Q. Duy, Jason K. Wang, Ross O'Hagan, Artine Arzani, Arsalan Haghdel, Clarence C. Lim, Vwaire Orhurhu, Ivan Urits, Anh L. Ngo, Benjamin S. Glicksberg, Kathryn T. Hall, Darshan Mehta, Richard S. Cooper, Girish N. Nadkarni

Faculty Publications

Objective Multiple clinical trials fail to identify clinically measurable health benefits of daily multivitamin and multimineral (MVM) consumption in the general adult population. Understanding the determinants of widespread use of MVMs may guide efforts to better educate the public about effective nutritional practices. The objective of this study was to compare self-reported and clinically measurable health outcomes among MVM users and non-users in a large, nationally representative adult civilian non-institutionalised population in the USA surveyed on the use of complementary health practices. Design Cross-sectional analysis of the effect of MVM consumption on self-reported overall health and clinically measurable health outcomes. …

Prevalence Of Eating Disorder Risk And Body Image Dissatisfaction Among Rotc Cadets, Allison Smith, Dawn Emerson, Zachary Winkelmann, Devin Potter, Toni Torres-Mcgehee

Faculty Publications

Injury risk is multifactorial including non-modifiable and modifiable factors such as nutrition and mental health. The purpose of this study was to estimate eating disorder risk and body image (BI) dissatisfaction among Reserve Officers Training Corps (ROTC) cadets. A total of 102 (male: = 75, female: = 27; age: 20 ± 2 years) ROTC cadets self-reported height, current and ideal weight, and completed the Eating Attitudes Test-26 (EAT-26) and self-perceived BI current and perceived sex-specific figural stimuli. The overall eating disorder risk for ROTC cadets was 32.4%. No significant differences were found when comparing sex, ethnicity, or military branch. Overall …

Service Gifts, Collective Social Connection And Reciprocity, Yihui (Elina) Tang, Christian Hinsch, Donald J. Lund, Husni Kharouf

Faculty Publications

Purpose: This study aims to investigate the process of service gifting (i.e. unexpected upgrades or benefits) and examine why service gifts do not always result in firm-beneficial reciprocal behaviors from consumers. Design/methodology/approach: Through a series of three studies including both scenario-based and game-theory-based experiments, this research proposes and empirically validates a conceptual model that examines the effect of service gifts on firm-beneficial reciprocal behaviors, and the role of collective social connection and norm of positive reciprocity (NPR) in this process. Findings: The findings of this research show that the consumer’s feelings of collective social connection mediate the link between the …

Uncertain Regional Urbanism In Venezuela. Government, Infrastructure And Environment, Fabio Capra-Ribeiro

Faculty Publications

Uncertain Regional Urbanism in Venezuela explores the changes cities face when they become metropolises, forming expanding regions which create both potential and problems within settlements. To do so, it focuses on three metropolitan areas located in Venezuela’s Center-North region: Caracas, Maracay and Valencia, designated as "Camava."

Considering three core topics, government and territorial administration, infrastructure and environment, as well as looking at the reciprocal impact, this book describes and analyzes the determinant variables that characterize the phenomenon of regional urbanization in this area and in the wider Global South. It includes documentary research, semi-structured interviews and Delphi methodology, involving a …

Facilitating A Path To New Teacher Certification Amid The Covid-19 Pandemic: Unpacking States’ Unchanged-New Flex” Guidelines, Laura E. Slay, Jacqueline Riley, Karyn E. Miller

Faculty Publications

In the United States, amid the COVID-19 pandemic, schools and testing centers were forced to close on-site locations. With teacher candidates no longer able to complete clinical teaching or take certification exams in person, states created new recommendations for facilitating a pathway to teacher certification. Specifically, 19 states provided guidelines that allowed educator preparation programs (EPPs) flexibility in how teacher candidates completed existing certification requirements. By analyzing summaries of these states’ guidelines, themes of time, technology, flexibility/nonflexibility, and EPPs emerged. Using a comprehensive lens, this brief examines the role and implications of each of these themes in teacher certification during …

Plant Invasion Impacts On Fungal Community Structure And Function Depend On Soil Warming And Nitrogen Enrichment, Mark A. Anthony, K. A. Stinson, Jessica A. M. Moore, Serita D. Frey

Faculty Publications

The impacts of invasive species on biodiversity may be mitigated or exacerbated by abiotic environmental changes. Invasive plants can restructure soil fungal communities with important implications for native biodiversity and nutrient cycling, yet fungal responses to invasion may depend on numerous anthropogenic stressors. In this study, we experimentally invaded a long-term soil warming and simulated nitrogen deposition experiment with the widespread invasive plant Alliaria petiolata (garlic mustard) and tested the responses of soil fungal communities to invasion, abiotic factors, and their interaction. We focused on the phytotoxic garlic mustard because it suppresses native mycorrhizae across forests of North America. We …

Parsing The Impact Of Alice And The Peg, Colleen Chien, Nicholas Halkowski, Maria He, Rodney Swartz

Faculty Publications

Almost two years have passed since the USPTO issued its January 2019 Patent Eligibility Guidance (PEG), itself a response to the Supreme Court’s Alice decision, and what many perceived as its destabilizing impact on the certainty of patent prosecutions. Leveraging new data releases, we report on trends in prosecution following the USPTO’s PEG and the Guidance on 112, finding 1) a decline in subject matter rejections and stabilization of subject matter appeals, 2) no discernable increase in 112 rejections, 3) no evidence that small entities were being left behind in Alice-impacted art units by forum shopping by large entities, …