Open Access. Powered by Scholars. Published by Universities.®

Digital Commons Network™

Open Access. Powered by Scholars. Published by Universities.®

Faculty Publications

Discipline
Institution
Keyword
Publication Year
File Type

Articles 8371 - 8400 of 10793

Full-Text Articles in Entire DC Network

Poverty And The Multiple Stakeholder Challenge For Global Leaders, C. Reade, Anne Marie Todd, A. Osland, J. Osland Jan 2008

Poverty And The Multiple Stakeholder Challenge For Global Leaders, C. Reade, Anne Marie Todd, A. Osland, J. Osland

Faculty Publications

The article presents a case study in which business leaders deal with challenging problems related to poverty, involving multiple stakeholders. This emphasizes the importance of training prospective global leaders to manage stakeholder relationships and engage in stakeholder dialogue. The authors highlight the stakeholder role played by nongovernmental organizations and include a simulation that develops stakeholder dialogue skills. They identify practical lessons and assumptions underlying business education that are not shared by all stakeholders in the context of poverty.

Go to article

The N-Terminus Of Apolipoprotein A-V Adopts A Helix-Bundle Molecular Architecture, Kasuen Wong, J. A. Beckstead, D. Lee, P. M.M. Weers, E. Guigard, C. M. Kay, R. O. Ryan Jan 2008

The N-Terminus Of Apolipoprotein A-V Adopts A Helix-Bundle Molecular Architecture, Kasuen Wong, J. A. Beckstead, D. Lee, P. M.M. Weers, E. Guigard, C. M. Kay, R. O. Ryan

Faculty Publications

Previous studies of recombinant full-length human apolipoprotein A-V (apoA-V) provided evidence of the presence of two independently folded structural domains. Computer-assisted sequence analysis and limited proteolysis studies identified an N-terminal fragment as a candidate for one of the domains. C-Terminal truncation variants in this size range, apoA-V(1-146) and apoA-V(1-169), were expressed in Escherichia coli and isolated. Unlike full-length apoA-V or apoA-V(1-169), apoA-V(1-146) was soluble in neutral-pH buffer in the absence of lipid. Sedimentation equilibrium analysis yielded a weight-average molecular weight of 18811, indicating apoA-V(1-146) exists as a monomer in solution. Guanidine HCl denaturation experiments at pH 3.0 yielded a one-step …

Go to article

Stochastic Hard-Sphere Dynamics For Hydrodynamics Of Non-Ideal Fluids, Alejandro Garcia, A. Donev, B. J. Alder Jan 2008

Stochastic Hard-Sphere Dynamics For Hydrodynamics Of Non-Ideal Fluids, Alejandro Garcia, A. Donev, B. J. Alder

Faculty Publications

A novel stochastic fluid model is proposed with a nonideal structure factor consistent with compressibility, and adjustable transport coefficients. This stochastic hard sphere dynamics (SHSD) algorithm is a modification of the direct simulation Monte Carlo algorithm and has several computational advantages over event-driven hard-sphere molecular dynamics. Surprisingly, SHSD results in an equation of state and a pair correlation function identical to that of a deterministic Hamiltonian system of penetrable spheres interacting with linear core pair potentials. The fluctuating hydrodynamic behavior of the SHSD fluid is verified for the Brownian motion of a nanoparticle suspended in a compressible solvent.

Go to article

Comparative Diet Of Three Sympatric Sceloporus In The Semiarid Zapotitlan Valley, Mexico, V. Hugo Serrano-Cardozo, J. A. Lemos-Espinal, Geoffrey R. Smith Jan 2008

Comparative Diet Of Three Sympatric Sceloporus In The Semiarid Zapotitlan Valley, Mexico, V. Hugo Serrano-Cardozo, J. A. Lemos-Espinal, Geoffrey R. Smith

Faculty Publications

No abstract provided.

Go to article

The Future Of The Economic Analysis Of Law In Latin America: A Proposal For Model Codes, Juan Javier Del Granado, M C. Mirow Jan 2008

The Future Of The Economic Analysis Of Law In Latin America: A Proposal For Model Codes, Juan Javier Del Granado, M C. Mirow

Faculty Publications

Nothing excites civilian lawyers and judges more than commissions for codification. Codification is more than an academic enterprise. Codification projects directly cut across the interface between law and life. ALACDE intends to harness this Latin American interest in codification to bring the economic approach to Latin America. A new-generation law and economics civil and commercial code will be a conscious project to restate Roman law's usefulness for coping with today's problems. Through law and economics, Roman law will renew itself. As a paradigmatic private-law system, Roman law is eminently amenable to a state-of-the-art fusion with law and economics. Sensitivity to …

Go to article

Time Reversal, Brian E. Anderson, Michele Griffa, Paul A. Johnson, Carene Larmat, Timothy J. Ulrich Jan 2008

Time Reversal, Brian E. Anderson, Michele Griffa, Paul A. Johnson, Carene Larmat, Timothy J. Ulrich

Faculty Publications

This article provides an historical overview of Time Reversal (TR), introduces its basic physics, addresses advantages and limitations, and describes some applications of this very active research area of acoustics. In the Geophysics Group at the Los Alamos National Laboratory, we conduct studies of TR of elastic waves in solids. Our work includes application of TR to nondestructive evaluation of materials, as well as to earthquake source characterization, and ground-based nuclear explosion monitoring. We emphasize the term elastic waves here to underscore that we include both compression and shear waves, in contrast to purely acoustic waves that are only compressional.

Go to article

Is There Something Unique About Marriage? The Relative Impact Of Marital Status, Relationship Quality, And Network Social Support On Ambulatory Blood Pressure And Mental Health, Wendy C. Birmingham, Julianne Holt-Lunstad, Brandon Q. Jones Jan 2008

Is There Something Unique About Marriage? The Relative Impact Of Marital Status, Relationship Quality, And Network Social Support On Ambulatory Blood Pressure And Mental Health, Wendy C. Birmingham, Julianne Holt-Lunstad, Brandon Q. Jones

Faculty Publications

Background Having close social relationships and being married specifically have been reliably associated with health benefits including lower morbidity and mortality. Purpose The purpose of this study was to examine the influence of marital status, relationship quality, and network support on measures of psychological and cardiovascular health. Method We examined ambulatory blood pressure (ABP) among 204 married and 99 single males and females (N=303). Results We found that both marital status and marital quality were important. Married individuals had greater satisfaction with life (SWL) and blood pressure dipping than single individuals. High marital quality was associated with lower ABP, lower …

Go to article

Donald Harman Akenson. Some Family: The Mormons And How Humanity Keeps Track Of Itself, J. Michael Hunter Jan 2008

Donald Harman Akenson. Some Family: The Mormons And How Humanity Keeps Track Of Itself, J. Michael Hunter

Faculty Publications

Review by J. Michael Hunter of Donald Harman Akenson’s Some Family: The Mormons and How Humanity Keeps Track of Itself (Montreal: McGill University / Kingston, Ontario: Queen’s University Press, 2007).

Go to article

A Commitment To Excellence, Meredith Jones-Gray Jan 2008

A Commitment To Excellence, Meredith Jones-Gray

Faculty Publications

No abstract provided.

Go to article

Macroh2a1 Knockdown Effects On The Peg3 Imprinted Domain, Jung Ha Choo, Jeong Do Kim, Joomyeong Kim Dec 2007

Macroh2a1 Knockdown Effects On The Peg3 Imprinted Domain, Jung Ha Choo, Jeong Do Kim, Joomyeong Kim

Faculty Publications

Background: MacroH2A1 is a histone variant that is closely associated with the repressed regions of chromosomes. A recent study revealed that this histone variant is highly enriched in the inactive alleles of Imprinting Control Regions (ICRs). Results: The current study investigates the potential roles of macroH2A1 in genomic imprinting by lowering the cellular levels of the macroH2A1 protein. RNAi-based macroH2A1 knockdown experiments in Neuro2A cells changed the expression levels of a subset of genes, including Peg3 and Usp29 of the Peg3 domain. The expression of these genes was down-regulated, rather than up-regulated, in response to reduced protein levels of the …

Go to article

Regional Body Composition In College-Aged Caucasians From Anthropometric Measures, Robert T. Davidson, Cameron B. Ritchie Dec 2007

Regional Body Composition In College-Aged Caucasians From Anthropometric Measures, Robert T. Davidson, Cameron B. Ritchie

Faculty Publications

Quantitating fat and lean tissue in isolated body regions may be helpful or required in obesity and health-outcomes research. However, current methods of regional body composition measurement require specialized, expensive equipment such as that used in computed tomography or dual energy x-ray absorptiometry (DEXA). Simple body size or circumference measurement relationships to body composition have been developed but are limited to whole-body applications. We investigated relationships between body size measurements and regional body composition. Methods Using DEXA technology we determined the fat and lean tissue composition for six regions of the body in predominantly Caucasian, college-aged men (n = 32) …

Go to article

Fractionation Of The Rice Bran Layer And Quantification Of Vitamin E, Oryzanol, Protein, And Rice Bran Saccharide, Rebecca Schramm, Alicia Abadie, Na Hua, Zhimin Xu, Marybeth Lima Dec 2007

Fractionation Of The Rice Bran Layer And Quantification Of Vitamin E, Oryzanol, Protein, And Rice Bran Saccharide, Rebecca Schramm, Alicia Abadie, Na Hua, Zhimin Xu, Marybeth Lima

Faculty Publications

Value-added processing with respect to rice milling has traditionally treated the rice bran layer as a homogenous material that contains significant concentrations of high-value components of interest for pharmaceutical and nutraceutical applications. Investigators have shown that high-value components in the rice bran layer vary from differences in kernel-thickness, bran fraction, rice variety, and environmental conditions during the growing season. The objectives of this study were to quantify the amount of rice bran removed at pre-selected milling times and to correlate the amount of rice bran removed at each milling time with the concentration of vitamin E, gamma-oryzanol, rice bran saccharide, …

Go to article

Arabian Sea Response To Monsoon Variations, Raghu Murtugudde, Richard Seager, Prasad Thoppil Dec 2007

Arabian Sea Response To Monsoon Variations, Raghu Murtugudde, Richard Seager, Prasad Thoppil

Faculty Publications

This study aims to quantify the impact of strong monsoons on the mixed layer heat budget in the Arabian Sea by contrasting forced ocean general circulation model simulations with composite strong and weak monsoon winds. Strong (weak) monsoons are defined as years with zonal component of the Somali Jet being greater (smaller) by more than a standard deviation of the long-term mean of the National Centers for Environmental Prediction reanalysis winds. Coastal upwelling is shown to be demonstrably stronger for strong monsoons leading to significant surface cooling, shallower thermoclines, and deeper mixed layers. A coupled ecosystem model shows that surface …

Go to article

Mobile Dna Elements In Primate And Human Evolution, Jinchuan Xing, David J. Witherspoon, David A. Ray, Mark A. Batzer, Lynn B. Jorde Dec 2007

Mobile Dna Elements In Primate And Human Evolution, Jinchuan Xing, David J. Witherspoon, David A. Ray, Mark A. Batzer, Lynn B. Jorde

Faculty Publications

Roughly 50% of the primate genome consists of mobile, repetitive DNA sequences such as Alu and LINE1 elements. The causes and evolutionary consequences of mobile element insertion, which have received considerable attention during the past decade, are reviewed in this article. Because of their unique mutational mechanisms, these elements are highly useful for answering phylogenetic questions. We demonstrate how they have been used to help resolve a number of questions in primate phylogeny, including the human-chimpanzee- gorilla trichotomy and New World primate phylogeny. Alu and LINE1 element insertion polymorphisms have also been analyzed in human populations to test hypotheses about …

Go to article

A Regional Modeling Study Of The Entraining Mediterranean Outflow, Xiaobiao Xu, E. P. Chassignet, J. F. Price, T. M. Özgökmen, H. Peters Dec 2007

A Regional Modeling Study Of The Entraining Mediterranean Outflow, Xiaobiao Xu, E. P. Chassignet, J. F. Price, T. M. Özgökmen, H. Peters

Faculty Publications

[1] We have evaluated a regional-scale simulation of the Mediterranean outflow by comparison with field data obtained in the 1988 Gulf of Cadiz Expedition. Our ocean model is based upon the Hybrid Coordinate Ocean Model (HYCOM) and includes the Richardson number-dependent entrainment parameterization of Xu et al. (2006). Given realistic topography and sufficient resolution, the model reproduces naturally the major, observed features of the Mediterranean outflow in the Gulf of Cadiz: the downstream evolution of temperature, salinity, and velocity profiles, the mean path and the spreading of the outflow plume, and most importantly, the localized, strong entrainment that has been …

Go to article

Shared Parasitoids In A Metacommunity: Indirect Interactions Inhibit Herbivore Membership In Local Communities, James T. Cronin Dec 2007

Shared Parasitoids In A Metacommunity: Indirect Interactions Inhibit Herbivore Membership In Local Communities, James T. Cronin

Faculty Publications

The interaction between species, mediated by a shared natural enemy (i.e., apparent competition), has been the subject of much theoretical and empirical investigation. However, we lack field experiments that assess the importance of apparent competition to metacommunity structure. Here, I conducted a series of field experiments to test whether apparent competition, mediated by shared egg parasitoids (Anagrus nigriventris and A. columbi), occurs between two abundant planthopper species (Delphacodes scolochloa and Prokelisia crocea) of the North American Great Plains. The two planthoppers feed on different plant species within prairie potholes (wet depressions) and, thus, do not interact directly. At the scale …

Go to article

From Population Sources To Sieves: The Matrix Alters Host-Parasitoid Source-Sink Structure, James T. Cronin Dec 2007

From Population Sources To Sieves: The Matrix Alters Host-Parasitoid Source-Sink Structure, James T. Cronin

Faculty Publications

Field experiments that examine the impact of immigration, emigration, or landscape structure (e.g., the composition of the matrix) on the source-sink dynamics of fragmented populations are scarce. Here, planthoppers (Prokelisia crocea) and egg parasitoids (Anagrus columbi) were released among host-plant patches that varied in structural (caged, isolated, or in a network of other patches) and functional (mudflat matrix that impedes dispersal vs. brome-grass matrix that facilitates dispersal) connectivity. Planthoppers and parasitoids on caged patches exhibited density-dependent growth rates, achieved high equilibrium densities, and rarely went extinct. Therefore, experimental cordgrass patches were classified as population sources. Because access to immigrants did …

Go to article

The Need For Proper Vouchering In Phylogenetic Studies Of Birds, A. Townsend Peterson, Robert G. Moyle, Árpád S. Nyári, Mark B. Robbins, Robb T. Brumfield, J. V. Remsen Dec 2007

The Need For Proper Vouchering In Phylogenetic Studies Of Birds, A. Townsend Peterson, Robert G. Moyle, Árpád S. Nyári, Mark B. Robbins, Robb T. Brumfield, J. V. Remsen

Faculty Publications

No abstract provided.

Go to article

Fluxes Of Iron And Manganese Across The Sediment-Water Interface Under Various Redox Conditions, Svetlana V. Pakhomova, Per O.J. Hall, Mikhail Yu Kononets, Alexander G. Rozanov, Anders Tengberg, Andrei V. Vershinin Dec 2007

Fluxes Of Iron And Manganese Across The Sediment-Water Interface Under Various Redox Conditions, Svetlana V. Pakhomova, Per O.J. Hall, Mikhail Yu Kononets, Alexander G. Rozanov, Anders Tengberg, Andrei V. Vershinin

Faculty Publications

Fluxes of dissolved forms of iron and manganese across the sediment-water interface were studied in situ in the Gulf of Finland and the Vistula Lagoon (Baltic Sea), and in the Golubaya Bay (Black Sea) from 2001 to 2005. Fluxes were measured using chamber incubations, and sediment cores were collected and sliced to assess the porewater and solid phase metal distribution at different depths. Measured and calculated benthic fluxes of manganese and iron were directed out of sediment for all sites and were found to vary between 70-4450 and 5-1000 μmole m- 2 day- 1 for manganese and iron, respectively. The …

Go to article

Impact Of Energy Intake, Physical Activity, And Population-Wide Weight Loss On Cardiovascular Disease And Diabetes Mortality In Cuba, 1980-2005, Manuel Franco, Pedro Orduñez, Benjamín Caballero, José A. Tapia Granados, Mariana Lazo, José Luís Bernal, Eliseo Guallar, Richard S. Cooper Dec 2007

Impact Of Energy Intake, Physical Activity, And Population-Wide Weight Loss On Cardiovascular Disease And Diabetes Mortality In Cuba, 1980-2005, Manuel Franco, Pedro Orduñez, Benjamín Caballero, José A. Tapia Granados, Mariana Lazo, José Luís Bernal, Eliseo Guallar, Richard S. Cooper

Faculty Publications

Cuba's economic crisis of 1989-2000 resulted in reduced energy intake, increased physical activity, and sustained population-wide weight loss. The authors evaluated the possible association of these factors with mortality trends. Data on per capita daily energy intake, physical activity, weight loss, and smoking were systematically retrieved from national and local surveys. National vital statistics from 1980-2005 were used to assess trends in mortality from diabetes, coronary heart disease, stroke, cancer, and all causes. The crisis reduced per capita daily energy intake from 2,899 calories to 1,863 calories. During the crisis period, the proportion of physically active adults increased from 30% …

Go to article

Density Gradient Enrichment Of Escherichia Coli Conditional Msba Mutants, William T. Doerrler Dec 2007

Density Gradient Enrichment Of Escherichia Coli Conditional Msba Mutants, William T. Doerrler

Faculty Publications

Insight into the mechanism of lipid transport to the outer membrane of gram-negative bacteria has been hampered by the lack of an effective genetic screen for defective mutants. This work demonstrates an enrichment of conditional mutants defective in lipopolysaccharide export by Ludox density gradient centrifugation and selection for detergent resistance. New temperature-sensitive mutants with lipid export defects were isolated with single missense mutations in msbA. The results demonstrate the power of this approach for the study of lipid export in Escherichia coli. Copyright © 2007, American Society for Microbiology. All Rights Reserved.

Go to article

Cold Seeps And Associated Communities Of The Gulf Of Mexico, Charles Fisher, Harry Roberts, Erik Cordes, Bernie Bernard Dec 2007

Cold Seeps And Associated Communities Of The Gulf Of Mexico, Charles Fisher, Harry Roberts, Erik Cordes, Bernie Bernard

Faculty Publications

No abstract provided.

Go to article

Development Of New Simple Sequence Repeat Markers For Pearl Millet, O. P. Yadav, S. E. Mitchell, A. Zamora, Theresa M. Fulton, Stephen Kresovich Dec 2007

Development Of New Simple Sequence Repeat Markers For Pearl Millet, O. P. Yadav, S. E. Mitchell, A. Zamora, Theresa M. Fulton, Stephen Kresovich

Faculty Publications

No abstract provided.

Go to article

Species Invasion Shifts The Importance Of Predator Dependence, Blaine D. Griffen, David G. Delaney Dec 2007

Species Invasion Shifts The Importance Of Predator Dependence, Blaine D. Griffen, David G. Delaney

Faculty Publications

The strength of interference between foraging individuals can influence per capita consumption rates, with important consequences for predator and prey populations and system stability. Here we demonstrate how the replacement of a previously established invader, the predatory crab Carcinus maenas, by the recently invading predatory crab Hemigrapsus sanguineus shifts predation from a species that experiences strong predator interference (strong predator dependence) to one that experiences weak predator interference (weak predator dependence). We demonstrate using field experiments that differences in the strength of predator dependence persist for these species both when they forage on a single focal prey species only (the …

Go to article

Hunting For Hydrothermal Vents Along The Galápagos Spreading Center, Rachel M. Haymon, Edward T. Baker, Joseph A. Resing, Scott M. White, Ken C. Macdonald Dec 2007

Hunting For Hydrothermal Vents Along The Galápagos Spreading Center, Rachel M. Haymon, Edward T. Baker, Joseph A. Resing, Scott M. White, Ken C. Macdonald

Faculty Publications

No abstract provided.

Go to article

Laboratory Method To Estimate Rain-Induced Splitting In Cultivated Blueberries, Donna A. Marshall-Shaw, James M. Spiers, Stephen J. Stringer, Kenneth J. Curry Dec 2007

Laboratory Method To Estimate Rain-Induced Splitting In Cultivated Blueberries, Donna A. Marshall-Shaw, James M. Spiers, Stephen J. Stringer, Kenneth J. Curry

Faculty Publications

Preharvest rainfall that occurs when fruit are fully ripe or approaching full ripeness can result in detrimental fruit splitting in rabbiteye and southern highbush blueberries. This study was initiated to develop a laboratory method to model rain-related incidence of splitting in cultivated blueberries with the goal of predicting the incidence of splitting in blueberry cultivars and selections. Multiyear field surveys of rabbiteye and southern highbush cultivars show that the incidence of rain-related splitting is strongly cultivar-dependent. Laboratory values for forced splitting and naturally occurring rain-related field splitting data show a strong correlation indicating that the incidence of fruit splitting can …

Go to article

Evalution Of The Efficacy Of The Photosystem Ii Inhibitor Dcmu In Periphyton And Its Effects On Nontarget Microorganisms And Extracellular Enzymatic Reactions, Steven N. Francoeur, Audrey C. Johnson, Kevin A. Kuehn, Robert K. Neely Dec 2007

Evalution Of The Efficacy Of The Photosystem Ii Inhibitor Dcmu In Periphyton And Its Effects On Nontarget Microorganisms And Extracellular Enzymatic Reactions, Steven N. Francoeur, Audrey C. Johnson, Kevin A. Kuehn, Robert K. Neely

Faculty Publications

We examined the efficacy of the photosystem II inhibitor 3-(3,4-diclorophenyl)-1,1-dimethyl urea (DCMU) for inhibition of algal photosynthesis in periphyton associated with submerged decomposing litter of Typha angustifolia. We also investigated the possible nontarget effects of DCMU exposure on heterotrophic microorganisms (i.e., bacteria and fungi) and extracellular enzyme activity associated with decaying litter. Standing-dead Typha leaf litter was submerged for 34 and 73 d, returned to the laboratory, and used for controlled laboratory experiments that examined the effect of DCMU on algal ([14C]bicarbonate, pulse-amplitude modulated fluorometry), bacterial ([3H]leucine), and fungal ([14C]acetate) production. Simultaneous assays …

Go to article

A Statistical Appraisal Of Disproportional Versus Proportional Microbial Source Tracking Libraries, Brian J. Robinson, Kerry J. Ritter, Rudolph D. Ellender Dec 2007

A Statistical Appraisal Of Disproportional Versus Proportional Microbial Source Tracking Libraries, Brian J. Robinson, Kerry J. Ritter, Rudolph D. Ellender

Faculty Publications

Library-based microbial source tracking (MST) can assist in reducing or eliminating fecal pollution in waters by predicting sources of fecal-associated bacteria. Library-based MST relies on an assembly of genetic or phenotypic "fingerprints" from pollution-indicative bacteria cultivated from known sources to compare with and identify fingerprints of unknown origin. The success of the library-based approach depends on how well each source candidate is represented in the library and which statistical algorithm or matching criterion is used to match unknowns. Because known source libraries are often built based on convenience or cost, some library sources may contain more representation than others. Depending …

Go to article

Effect Of Endothelin 1 Genotype On Blood Pressure Is Dependent On Physical Activity Or Fitness Levels, Tuomo Rankinen, Timothy S. Church, Treva Rice, Nathan Markward, Arthur S. Leon, Dabeeru C. Rao, James S. Skinner, Steven N. Blair, Claude Bouchard Dec 2007

Effect Of Endothelin 1 Genotype On Blood Pressure Is Dependent On Physical Activity Or Fitness Levels, Tuomo Rankinen, Timothy S. Church, Treva Rice, Nathan Markward, Arthur S. Leon, Dabeeru C. Rao, James S. Skinner, Steven N. Blair, Claude Bouchard

Faculty Publications

Contributions of the DNA sequence variation at the endothelin 1 locus to the risk of hypertension and to endurance training-induced changes in blood pressure were investigated in the Aerobics Center Longitudinal Study and the Health, Risk Factors, Exercise Training and Genetics Family Study cohorts. We identified 586 normotensive control subjects and 607 incident hypertensive case subjects from the Aerobics Center Longitudinal Study cohort (all whites) who were normotensive and healthy at their first clinical visit. The case subjects were diagnosed with hypertension during an average follow-up of 9.5 years, whereas the control subjects remained normotensive. The allele and genotype frequencies …

Go to article

Improving Protein Crystal Quality By Selective Removal Of A Ca 2+-Dependent Membrane-Insertion Loop, David B. Neau, Nathaniel C. Gilbert, Sue G. Bartlett, Adam Dassey, Marcia E. Newcomer Nov 2007

Improving Protein Crystal Quality By Selective Removal Of A Ca 2+-Dependent Membrane-Insertion Loop, David B. Neau, Nathaniel C. Gilbert, Sue G. Bartlett, Adam Dassey, Marcia E. Newcomer

Faculty Publications

Lipoxygenases (LOXs) catalyze the regiospecific and stereospecific dioxygenation of polyunsaturated membrane-embedded fatty acids. A Ca 2+-dependent membrane-binding function was localized to the amino-terminal C2-like domain of 8R-lipoxygenase (8R-LOX) from the soft coral Plexaura homomalla. The 3.2 Å crystal structure of 8R-LOX and spectroscopic data suggested that Ca2+ stabilizes two membrane-insertion loops. Analysis of the protein packing contacts in the crystal lattice indicated that the conformation of one of the two loops complicated efforts to improve the resolution of the X-ray data. A deletion mutant of 8R-LOX in which the corresponding membrane-insertion loop is absent (Δ41-45:GSLOX) was engineered. Removal of the …

Go to article

First Prev Next Last