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Legal Forms And The Common Law Of Patents, Craig Allen Nard Jan 2010

Legal Forms And The Common Law Of Patents, Craig Allen Nard

Faculty Publications

The question of institutional choice is important in all areas of the law, but particularly in the context of patent law with its divergent stakeholders, decentralized variance among industries regarding how the patent system is viewed and relied upon, and a persistent focus on reform in recent years. For over two hundred years, the courts have been the dominant force in the development of patent law. It should therefore come as no surprise to learn that a significant portion of American patent law, including some of the most important and controversial patent law doctrines, is either built upon judicial interpretation …


Substrate And Cover Object Choice By The Red-Backed Salamander (Plethodon Cinereus), J. P. Iverson, Geoffrey R. Smith Jan 2010

Substrate And Cover Object Choice By The Red-Backed Salamander (Plethodon Cinereus), J. P. Iverson, Geoffrey R. Smith

Faculty Publications

No abstract provided.


Synaptically Activated Burst-Generating Conductances May Underlie A Group-Pacemaker Mechanism For Respiratory Rhythm Generation In Mammals, Christopher A. Del Negro, John A. Hayes, Ryland W. Pace, Benjamin R. Brush, Ryoichi Teruyama, Jack L. Feldman Jan 2010

Synaptically Activated Burst-Generating Conductances May Underlie A Group-Pacemaker Mechanism For Respiratory Rhythm Generation In Mammals, Christopher A. Del Negro, John A. Hayes, Ryland W. Pace, Benjamin R. Brush, Ryoichi Teruyama, Jack L. Feldman

Faculty Publications

Breathing, chewing, and walking are critical life-sustaining behaviors in mammals that consist essentially of simple rhythmic movements. Breathing movements in particular involve the diaphragm, thorax, and airways but emanate from a network in the lower brain stem. This network can be studied in reduced preparations in vitro and using simplified mathematical models that make testable predictions. An iterative approach that employs both in vitro and in silico models argues against canonical mechanisms for respiratory rhythm in neonatal rodents that involve reciprocal inhibition and pacemaker properties. We present an alternative model in which emergent network properties play a rhythmogenic role. Specifically, …


Histone H2a.Z Regulates The Expression Of Several Classes Of Phosphate Starvation Response Genes But Not As A Transcriptional Activator, Aaron P. Smith, Ajay Jain, Roger B. Deal, Vinay K. Nagarajan, Michael D. Poling, Kashchandra G. Raghothama, Richard B. Meagher Jan 2010

Histone H2a.Z Regulates The Expression Of Several Classes Of Phosphate Starvation Response Genes But Not As A Transcriptional Activator, Aaron P. Smith, Ajay Jain, Roger B. Deal, Vinay K. Nagarajan, Michael D. Poling, Kashchandra G. Raghothama, Richard B. Meagher

Faculty Publications

Phosphate (Pi) availability is a major constraint to plant growth. Consequently, plants have evolved complex adaptations to tolerate low Pi conditions. Numerous genes implicated in these adaptations have been identified, but their chromatin-level regulation has not been investigated. The nuclear actin-related protein ARP6 is conserved among all eukaryotes and is an essential component of the SWR1 chromatin remodeling complex, which regulates transcription via deposition of the H2A.Z histone variant into chromatin. Here, we demonstrate that ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis (Arabidopsis thaliana). The loss of H2A.Z at …


Intracellular Ph Regulation In Isolated Trout Gill Mitochondrion-Rich (Mr) Cell Subtypes: Evidence For Na+/H+ Activity, Scott K. Parks, Martin Tresguerres, Fernando Galvez, Greg G. Goss Jan 2010

Intracellular Ph Regulation In Isolated Trout Gill Mitochondrion-Rich (Mr) Cell Subtypes: Evidence For Na+/H+ Activity, Scott K. Parks, Martin Tresguerres, Fernando Galvez, Greg G. Goss

Faculty Publications

We have studied intracellular pH (pHi) recovery in isolated trout gill mitochondrion-rich (MR) cells following acidification by the NH4Cl pre-pulse technique. Within a mixed MR cell population, one cell type displayed Na+-independent pHi recovery while the other cell type lacked a Na+-independent pHi recovery. Cells displaying Na+ independent recovery exhibited a significantly higher buffering capacity compared to cells lacking Na+-independent pHi recovery. Cells displaying Na+ independent recovery were identified as PNA+ (peanut lectin agluttinin binding) MR cells while those unable to recover were identified as PNA- (non-peanut lectin agluttinin binding) MR cells. Therefore, recovery from acidification in the absence of …


Social Status Regulates Kisspeptin Receptor Mrna In The Brain Of Astatotilapia Burtoni, Brian P. Grone, Karen P. Maruska, Wayne J. Korzan, Russell D. Fernald Jan 2010

Social Status Regulates Kisspeptin Receptor Mrna In The Brain Of Astatotilapia Burtoni, Brian P. Grone, Karen P. Maruska, Wayne J. Korzan, Russell D. Fernald

Faculty Publications

The brain controls reproduction in response to relevant external and internal cues. Central to this process in vertebrates is gonadotropin-releasing hormone (GnRH1) produced in neurons of the hypothalamic-preoptic area (POA). GnRH1 released from the POA stimulates pituitary release of gonadotropins, which in males causes sperm production and concomitant steroid hormone release from the testes. Kisspeptin, a neuropeptide acting via the kisspeptin receptor (Kiss1r), increases GnRH1 release and is linked to development of the reproductive system in mammals and other vertebrates. In both fish and mammals, kiss1r mRNA levels increase in the brain around the time of puberty but the environmental …


Expression, Localization And Interaction Of Snare Proteins In Arabidopsis Are Selectively Altered By The Dark, Naohiro Kato, Huancan Bai Jan 2010

Expression, Localization And Interaction Of Snare Proteins In Arabidopsis Are Selectively Altered By The Dark, Naohiro Kato, Huancan Bai

Faculty Publications

A method to detect binary interactions among SNAREs, membrane proteins mediating vesicle fusion, in Arabidopsis cells was established. In this method, a pair of recombinant SNAREs is first expressed within Arabidopsis protoplasts at levels similar to their endogenous proteins in 96-well plates. Changes of the interaction are then detected by luminescence. Here, we report that the interaction of SYP122 and VAMP721, a SNARE pair mediating exocytosis, is enhanced when Arabidopsis protoplasts are incubated in the dark. Microscopic observation of plants expressing GFPSYP122 by the syp122 promoter suggests SYP122 is expressed in the root tip when the seedlings are grown in …


Syntheses Of Protoporphyrin-Ix Derivatives Bearing Extended Propionate Side-Chains, Robert T. Holmes, Jianming Lu, Celinah Mwakwari, Kevin M. Smith Jan 2010

Syntheses Of Protoporphyrin-Ix Derivatives Bearing Extended Propionate Side-Chains, Robert T. Holmes, Jianming Lu, Celinah Mwakwari, Kevin M. Smith

Faculty Publications

In order to investigate the relationship between depth within membranes of singlet oxygen generation and effectiveness of photodynamic therapy of tumors, analogs of protoporphyrin-IX 1 bearing five 4 and seven 5 carbon atoms (in place of the 3-carbon atom chain in 1) were synthesized from monopyrrole precursors.


Improved Peak Capacity For Ce Separations Of Enzyme Inhibitors With Activity-Based Detection Using Magnetic Bead Microreactors, Xiaoyan Yan, S. Douglass Gilman Jan 2010

Improved Peak Capacity For Ce Separations Of Enzyme Inhibitors With Activity-Based Detection Using Magnetic Bead Microreactors, Xiaoyan Yan, S. Douglass Gilman

Faculty Publications

A technique for separating and detecting enzyme inhibitors was developed using CE with an enzyme microreactor. The on-column enzyme microreactor was constructed using NdFeB magnet(s) to immobilize alkaline phosphatase-coated superparamagnetic beads (2.8 mm diameter) inside a capillary before the detection window. Enzyme inhibition assays were performed by injecting a plug of inhibitor into a capillary filled with the substrate, AttoPhos. Product generated in the enzyme microreactor was detected by LIF. Inhibitor zones electrophoresed through the capillary, passed through the enzyme microreactor, and were observed as negative peaks due to decreased product formation. The goal of this study was to improve …


Keynote Address: The T-Team, Michael P. Scharf Jan 2010

Keynote Address: The T-Team, Michael P. Scharf

Faculty Publications

No abstract provided.


The Torture Lawyers, Michael P. Scharf Jan 2010

The Torture Lawyers, Michael P. Scharf

Faculty Publications

This article recounts the story about how these four individuals intentionally cut off the government's primary experts on the Geneva Conventions, the Torture Convention, and customary international law from the decision making process. In doing so, they presented a one-sided and distorted view of U.S. obligations under international law that led to a widespread government policy and practice of torture. It also reveals how a trio of important Supreme Court precedents disrupted these plans, and ultimately swung the balance back in favor of compliance with international law.


Engaging With The Politics Of Determinist Environmental Thinking, Andrew Sluyter Jan 2010

Engaging With The Politics Of Determinist Environmental Thinking, Andrew Sluyter

Faculty Publications

No abstract provided.


Renegotiating Barbuda's Commons: Recent Changes In Barbudan Open-Range Cattle Herding, Andrew Sluyter, Amy E. Potter Jan 2010

Renegotiating Barbuda's Commons: Recent Changes In Barbudan Open-Range Cattle Herding, Andrew Sluyter, Amy E. Potter

Faculty Publications

Barbuda remains little developed and sparsely populated relative to its neighbors in the Leeward Lesser Antilles, a rather extraordinary and relatively unknown Caribbean place. Much of its distinctiveness derives from the communal land-tenure system, itself rooted in three centuries of open-range cattle herding. Yet, as revealed through interviews, newspaper archives, and landscape observations, openrange cattle herding has declined over the past three decades, with related changes in land tenure. As the new Barbuda Land Act came into effect in 2008, codifying the communal tenure system, the very landscape elements that manifest open-range herding have become obscure. In particular, the rock-walled …


The Hispanic Atlantic's Tasajo Trail, Andrew Sluyter Jan 2010

The Hispanic Atlantic's Tasajo Trail, Andrew Sluyter

Faculty Publications

Produced along the Río de la Plata during the nineteenth century, shipped to Havana, and consumed by African slaves, the salt-cured beef known as tasajo affected both of those places and, to some degree, the Atlantic world in general. Initial exploration of the tasajo trail that connected Buenos Aires and Cuba employs primary sources such as nineteenth-century descriptions and shipping records to characterize the landscapes, places, routes, and agents of the largely unexplored research territory of that anomalous commodity: one that, unlike others such as sugar, slaves not only produced but also consumed; one that underpinned more prominent, latitudinal transatlantic …


Brucella Species Survey In Polar Bears (Ursus Maritimus) Of Northern Alaska, Todd M. O'Hara, Darce Holcomb, Philip Elzer, Jessica Estepp, Quinesha Perry, Sue Hagius, Cassandra Kirk Jan 2010

Brucella Species Survey In Polar Bears (Ursus Maritimus) Of Northern Alaska, Todd M. O'Hara, Darce Holcomb, Philip Elzer, Jessica Estepp, Quinesha Perry, Sue Hagius, Cassandra Kirk

Faculty Publications

We report on the presence of specific antibodies to Brucella spp. and Yersinia enterocolitica in polar bears (Ursus maritimus) from northern Alaska (southern Beaufort Sea) during 2003-2006. Based on numerous known stressors (e.g., climate change and loss of sea ice habitat, contaminants), there is increased concern regarding the status of polar bears. Considering these changes, it is important to assess exposure to potentially pathogenic organisms and to improve understanding of transmission pathways. Brucella or specific antibodies to Brucella spp. has been reported in marine mammals. Various assays were used to elucidate the pathway or source of exposure (e.g., "marine" vs. …


Testing Protozoacidal Activity Of Ligand-Lytic Peptides Against Termite Gut Protozoa In Vitro (Protozoa Culture) And In Vivo (Microinjection Into Termite Hindgut), Claudia Husseneder, Amit Sethi, Lane Foil, Jennifer Delatte Jan 2010

Testing Protozoacidal Activity Of Ligand-Lytic Peptides Against Termite Gut Protozoa In Vitro (Protozoa Culture) And In Vivo (Microinjection Into Termite Hindgut), Claudia Husseneder, Amit Sethi, Lane Foil, Jennifer Delatte

Faculty Publications

We are developing a novel approach to subterranean termite control that would lead to reduced reliance on the use of chemical pesticides. Subterranean termites are dependent on protozoa in the hindguts of workers to efficiently digest wood. Lytic peptides have been shown to kill a variety of protozoan parasites (Mutwiri et al. 2000) and also protozoa in the gut of the Formosan subterranean termite, Coptotermes formosanus (Husseneder and Collier 2009). Lytic peptides are part of the nonspecific immune system of eukaryotes, and destroy the membranes of microorganisms (Leuschner and Hansel 2004). Most lytic peptides are not likely to harm higher …


A Phylogenetic Approach To Disentangling The Role Of Competition And Habitat Filtering In Community Assembly Of Neotropical Forest Birds, Juan Pablo Gómez, Gustavo A. Bravo, Robb T. Brumfield, José G. Tello, Carlos Daniel Cadena Jan 2010

A Phylogenetic Approach To Disentangling The Role Of Competition And Habitat Filtering In Community Assembly Of Neotropical Forest Birds, Juan Pablo Gómez, Gustavo A. Bravo, Robb T. Brumfield, José G. Tello, Carlos Daniel Cadena

Faculty Publications

Methods that assess patterns of phylogenetic relatedness, as well as character distribution and evolution, allow one to infer the ecological processes involved in community assembly. Assuming niche conservatism, assemblages should shift from phylogenetic clustering to evenness with decreasing geographic scale because the relative importance of mechanisms that shape assemblages is hypothesized to be scale-dependent. Whereas habitat filtering is more likely to act at regional scales because of increased habitat heterogeneity that allows sorting of ecologically similar species in contrasting environments, competition is more likely to act at local scales because low habitat heterogeneity provides few opportunities for niche partitioning. We …


Comparison Of Structure-Based And Threading-Based Approaches To Protein Functional Annotation, Michal Brylinski, Jeffrey Skolnick Jan 2010

Comparison Of Structure-Based And Threading-Based Approaches To Protein Functional Annotation, Michal Brylinski, Jeffrey Skolnick

Faculty Publications

To exploit the vast amount of sequence information provided by the Genomic revolution, the biological function of these sequences must be identified. As a practical matter, this is often accomplished by functional inference. Purely sequencebased approaches, particularly in the "twilight zone" of low sequence similarity levels, are complicated by many factors. For proteins, structure-based techniques aim to overcome these problems; however, most require high-quality crystal structures and suffer from complex and equivocal relations between protein fold and function. In this study, in extensive benchmarking, we consider a number of aspects of structure-based functional annotation: binding pocket detection, molecular function assignment …


Unofficial Legislative History Of The Biologics Price Competition And Innovation Act 2009, An, Erika Lietzan, Krista Hessler Carver, Jeffrey Elikan Jan 2010

Unofficial Legislative History Of The Biologics Price Competition And Innovation Act 2009, An, Erika Lietzan, Krista Hessler Carver, Jeffrey Elikan

Faculty Publications

On March 23, 2010, President Obama signed into law the Biologics Price Competition and Innovation Act of 2009 (BPCIA) which created a regulatory pathway for, and scheme for litigation of patent issues relating to, “biosimilar” biological products. This article discusses the history of the BPCIA and explains its provisions. Section I provides background and a history of the regulation of drugs and biological products in the United States. Section II describes the growing interest in biosimilar approval from the early 2000s through September 2006, when the legislative debate began in earnest. Section III describes the legislative and stakeholder process from …


Neuropoietin Activates Stat3 Independent Of Lifr Activation In Adipocytes, Ursula A. White, Jacqueline M. Stephens Jan 2010

Neuropoietin Activates Stat3 Independent Of Lifr Activation In Adipocytes, Ursula A. White, Jacqueline M. Stephens

Faculty Publications

Neuropoietin (NP) is a member of the gp130 cytokine family that is closely related to cardiotrophin-1(CT-1) and shares functional and structural features with other family members, including ciliary neurotrophic factor (CNTF) and cardiotrophin-like cytokine (CLC). Studies have shown that NPcan play a role in the development of the nervous system, as well as affect adipogenesis and fat cell function. However, the signaling mechanisms utilized by NP in adipocytes have not been examined. In our present studies, we demonstrate that NP-induced activation of STAT3 tyrosine phosphorylation is independent of leukemia inhibitory factor receptor (LIFR) phosphorylation and degradation. Although it is widely …


A Systems Model Of Exocytic Vesicles In Arabidopsis Pollen During Its Germination, Naohiro Kato Jan 2010

A Systems Model Of Exocytic Vesicles In Arabidopsis Pollen During Its Germination, Naohiro Kato

Faculty Publications

Abasic systems model for vesicle trafficking in Arabidopsis pollen tubes was constructed. The model was composed of transcriptome data and differential equations. The transcriptome data revealed some genes controlling vesicle trafficking in the pollen tubes, and the differential equations connected the molecular functions of the gene products. The computed pollen tube growth reasonably agreed with biological samples. Here, I expand the computational prediction into exocytic vesicles during pollen germination, which can be used to examine the accuracy of the systems model with biological samples. The computational analysis of the model predicts that the number of exocytic vesicles changes in an …


Evolutionary Studies Illuminate The Structural-Functional Model Of Plant Phytochromes, Sarah Mathews Jan 2010

Evolutionary Studies Illuminate The Structural-Functional Model Of Plant Phytochromes, Sarah Mathews

Faculty Publications

A synthesis of insights from functional and evolutionary studies reveals how the phytochrome photoreceptor system has evolved to impart both stability and flexibility. Phytochromes in seed plants diverged into three major forms, phyA, phyB, and phyC, very early in the history of seed plants. Two additional forms, phyE and phyD, are restricted to flowering plants and Brassicaceae, respectively. While phyC, D, and E are absent from at least some taxa, phyA and phyB are present in all sampled seed plants and are the principal mediators of red/far-red-induced responses. Conversely, phyC-E apparently function in concert with phyB and, where present, expand …


Nuclear Actin-Related Proteins At The Core Of Epigenetic Control, Richard B. Meagher, Muthugapatti K. Kandasamy, Aaron P. Smith, Elizabeth C. Mckinney Jan 2010

Nuclear Actin-Related Proteins At The Core Of Epigenetic Control, Richard B. Meagher, Muthugapatti K. Kandasamy, Aaron P. Smith, Elizabeth C. Mckinney

Faculty Publications

Nuclear Actin-Related Proteins (ARPs) and actin combine as heterodimers to bind a large helicase subunit and form a core complex essential to the assembly and function of most chromatin remodeling and modifying machines. They are the most common shared subunits of these large and diverse assemblies in eukaryotes. We recently argued that most nuclear ARPs evolved directly from actin prior to the divergence of the eukaryotic kingdoms and did not evolve from pre-existing ARPs.2 Arabidopsis plants defective in nuclear ARP4, ARP5, ARP6 or ARP7 have extreme developmental phenotypes. Our recent publication demonstrates that ARP5-defective plants are not only dwarfed and …


A Conserved Interaction Between The Sdi Domain Of Bre2 And The Dpy-30 Domain Of Sdc1 Is Required For Histone Methylation And Gene Expression, Paul F. South, Ian M. Fingerman, Douglas P. Mersman, Hai Ning Du, Scott D. Briggs Jan 2010

A Conserved Interaction Between The Sdi Domain Of Bre2 And The Dpy-30 Domain Of Sdc1 Is Required For Histone Methylation And Gene Expression, Paul F. South, Ian M. Fingerman, Douglas P. Mersman, Hai Ning Du, Scott D. Briggs

Faculty Publications

In Saccharomyces cerevisiae, lysine 4 on histone H3 (H3K4) is methylated by the Set1 complex (Set1C or COMPASS). Besides the catalytic Set1 subunit, several proteins that form the Set1C (Swd1, Swd2, Swd3, Spp1, Bre2, and Sdc1) are also needed to mediate proper H3K4 methylation. Until this study, it has been unclear how individual Set1C members interact and how this interaction may impact histone methylation and gene expression. In this study, Bre2 and Sdc1 are shown to directly interact, and it is shown that the association of this heteromeric complex is needed for proper H3K4 methylation and gene expression to occur. …


Targeted Gene Replacement By Homologous Recombination In Drosophila Stimulates Production Of Second-Site Mutations, Swarnava Roy, Craig M. Hart Jan 2010

Targeted Gene Replacement By Homologous Recombination In Drosophila Stimulates Production Of Second-Site Mutations, Swarnava Roy, Craig M. Hart

Faculty Publications

Gene replacement by homologous recombination is a powerful technique for generating mutations in Drosophila. While using this technique for the BEAF gene, we encountered non-targeted lethal mutations on the target chromosome that complicated the analysis of the BEAF mutations until they were discovered and removed by meiotic recombination. Subsequent experiments indicated that the gene-targeting method leads to a modest but significant three-fold increase in the rate of production of non-targeted lethal mutations. It is important to be aware of this phenomenon when using this method. © 2010 Landes Bioscience.


Endoreplication Controls Cell Fate Maintenance, Jonathan Bramsiepe, Katja Wester, Christina Weinl, Farshad Roodbarkelari, Remmy Kasili, John C. Larkin, Martin Hülskamp, Arp Schnittger Jan 2010

Endoreplication Controls Cell Fate Maintenance, Jonathan Bramsiepe, Katja Wester, Christina Weinl, Farshad Roodbarkelari, Remmy Kasili, John C. Larkin, Martin Hülskamp, Arp Schnittger

Faculty Publications

Cell-fate specification is typically thought to precede and determine cell-cycle regulation during differentiation. Here we show that endoreplication, also known as endoreduplication, a specialized cell-cycle variant often associated with cell differentiation but also frequently occurring in malignant cells, plays a role in maintaining cell fate. For our study we have used Arabidopsis trichomes as a model system and have manipulated endoreplication levels via mutants of cell-cycle regulators and overexpression of cell-cycle inhibitors under a trichome-specific promoter. Strikingly, a reduction of endoreplication resulted in reduced trichome numbers and caused trichomes to lose their identity. Live observations of young Arabidopsis leaves revealed …


Pr10 Expression In Maize And Its Effect On Host Resistance Against Aspergillus Flavus Infection And Aflatoxin Production, Zhi Yuan Chen, Robert L. Brown, Kenneth E. Damann, Thomas E. Cleveland Jan 2010

Pr10 Expression In Maize And Its Effect On Host Resistance Against Aspergillus Flavus Infection And Aflatoxin Production, Zhi Yuan Chen, Robert L. Brown, Kenneth E. Damann, Thomas E. Cleveland

Faculty Publications

Maize (Zea mays L.) is a major crop susceptible to Aspergillus flavus infection and subsequent contamination with aflatoxins, the potent carcinogenic secondary metabolites of the fungus. Protein profiles of maize genotypes resistant and susceptible to A. flavus infection and/or aflatoxin contamination have been compared, and several resistance-associated proteins have been found, including a pathogenesis-related protein 10 (PR10). In this study, RNA interference (RNAi) gene silencing technology was employed to further investigate the importance of PR10. An RNAi gene silencing vector was constructed and introduced into immature Hi II maize embryos through both bombardment and Agrobacterium infection procedures. PR10 expression was …


Development Of A Prototype Bayesian Network Model Representing The Relationship Between Fresh Market Yield And Some Agroclimatic Variables Known To Influence Storage Root Initiation In Sweetpotato, Arthur Villordon, Julio Solis, Don Labonte, Christopher Clark Jan 2010

Development Of A Prototype Bayesian Network Model Representing The Relationship Between Fresh Market Yield And Some Agroclimatic Variables Known To Influence Storage Root Initiation In Sweetpotato, Arthur Villordon, Julio Solis, Don Labonte, Christopher Clark

Faculty Publications

A prototype phenology-driven Bayesian belief network (BBN) model, named BxNET, was developed to represent the relationship between fresh market yield (U.S. #1 grade) and agroclimatic variables known to influence the critical storage root initiation stages in 'Beauregard' sweetpotato. This data-driven model was developed from experimental data collected over 3 years of field trials in which management variables were kept as uniform as possible. The BBN was developed assuming that soil moisture measured at the 15-cm depth was not a limiting variable during the first 20 days after transplanting, during which the onset of storage root initiation determined the majority of …


Chitosan On The Postharvest Control Of Soft Rot In 'Rama Forte' Persimmon, Patrícia Cia, Eliane Aparecida Benato, Sérgio Florentino Pascholati, Ely Oliveira Garcia Jan 2010

Chitosan On The Postharvest Control Of Soft Rot In 'Rama Forte' Persimmon, Patrícia Cia, Eliane Aparecida Benato, Sérgio Florentino Pascholati, Ely Oliveira Garcia

Faculty Publications

The objective of this research was to evaluate the effect of chitosan in addition to de-astringency process on the control of Rhizopus stolonifer in 'Rama Forte' persimmon and on in vitro mycelial growth. Persimmon were submitted to de-astringency process using CO2 (70% / 18 h), in hermetic chambers. Next, fruit were inoculated through subcuticular injections of a R. stolonifer spore suspension (3x105 spore mL-1) and 2 hours later at 25 °C immersed into chitosan (0, 0.5, 1.0, 1.5, and 2.0%), for 1 min. Fruit were stored at 25 °C / 80% RH, and checked for rot severity and incidence of …


Darwinian Populations And Natural Selection, Grant Ramsey, Hope Hollocher, Agustín Fuentes, Charles H. Pence, Edwin Siu Jan 2010

Darwinian Populations And Natural Selection, Grant Ramsey, Hope Hollocher, Agustín Fuentes, Charles H. Pence, Edwin Siu

Faculty Publications

No abstract provided.