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Role Of Adipose Tissue In The Pathogenesis And Treatment Of Metabolic Syndrome, David Sanchez-Infantes, Jacqueline M. Stephens Jan 2014

Role Of Adipose Tissue In The Pathogenesis And Treatment Of Metabolic Syndrome, David Sanchez-Infantes, Jacqueline M. Stephens

Faculty Publications

© Springer International Publishing Switzerland 2014. Adipocytes are highly specialized cells that play a major role in energy homeostasis in vertebrate organisms. Excess adipocyte size or number is a hallmark of obesity, which is currently a global epidemic. Obesity is not only the primary disease of fat cells, but also a major risk factor for the development of Type 2 diabetes, cardiovascular disease, hypertension, and metabolic syndrome (MetS). Today, adipocytes and adipose tissue are no longer considered passive participants in metabolic pathways. In addition to storing lipid, adipocytes are highly insulin sensitive cells that have important endocrine functions. Altering any …

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Natural Selection Canalizes Expression Variation Of Environmentally Induced Plasticity-Enabling Genes, Joseph R. Shaw, Thomas H. Hampton, Benjamin L. King, Andrew Whitehead, Fernando Galvez, Robert H. Gross, Nathan Keith, Emily Notch, Dawoon Jung, Stephen P. Glaholt, Celia Y. Chen, John K. Colbourne, Bruce A. Stanton Jan 2014

Natural Selection Canalizes Expression Variation Of Environmentally Induced Plasticity-Enabling Genes, Joseph R. Shaw, Thomas H. Hampton, Benjamin L. King, Andrew Whitehead, Fernando Galvez, Robert H. Gross, Nathan Keith, Emily Notch, Dawoon Jung, Stephen P. Glaholt, Celia Y. Chen, John K. Colbourne, Bruce A. Stanton

Faculty Publications

© 2014 The Author. Many organisms survive fluctuating and extreme environmental conditions by manifesting multiple distinct phenotypes during adulthood by means of developmental processes that enable phenotypic plasticity. We report on the discovery of putative plasticity-enabling genes that are involved in transforming the gill of the euryhaline teleost fish, Fundulus heteroclitus, from its freshwater to its seawater gill-type, a process that alters both morphology and function. Gene expression that normally enables osmotic plasticity is inhibited by arsenic. Gene sets defined by antagonistic interactions between arsenic and salinity show reduced transcriptional variation among individual fish, suggesting unusually accurate and precise regulatory …

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Combustion By-Products And Their Health Effects - Combustion Engineering And Global Health In The 21st Century: Issues And Challenges, Slawo Lomnicki, Brian Gullett, Tobias Stöger, Ian Kennedy, Jim Diaz, Tammy R. Dugas, Kurt Varner, Danielle J. Carlin, Barry Dellinger, Stephania A. Cormier Jan 2014

Combustion By-Products And Their Health Effects - Combustion Engineering And Global Health In The 21st Century: Issues And Challenges, Slawo Lomnicki, Brian Gullett, Tobias Stöger, Ian Kennedy, Jim Diaz, Tammy R. Dugas, Kurt Varner, Danielle J. Carlin, Barry Dellinger, Stephania A. Cormier

Faculty Publications

The 13th International Congress on Combustion By-Products and their Health Effects was held in New Orleans, Louisiana from May 15 to 18, 2013. The congress, sponsored by the Superfund Research Program, National Institute of Environmental Health Sciences, and National Science Foundation, brought together international academic and government researchers, engineers, scientists, and policymakers. With industrial growth, increased power needs and generation and coal consumption and their concomitant emissions, pernicious health effects associated with exposures to these emissions are on the rise. This congress provides a unique platform for interdisciplinary exchange and discussion of these topics. The formation, conversion, control, and health …

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Deficiency Of The Two-Pore-Domain Potassium Channel Trek-1 Promotes Hyperoxia-Induced Lung Injury, Andreas Schwingshackl, Bin Teng, Patrudu Makena, Manik Ghosh, Scott E. Sinclair, Charlean Luellen, Louisa Balasz, Cynthia Rovnaghi, Robert M. Bryan, Eric E. Lloyd, Elizabeth Fitzpatrick, Jordy S. Saravia, Stephania A. Cormier, Christopher M. Waters Jan 2014

Deficiency Of The Two-Pore-Domain Potassium Channel Trek-1 Promotes Hyperoxia-Induced Lung Injury, Andreas Schwingshackl, Bin Teng, Patrudu Makena, Manik Ghosh, Scott E. Sinclair, Charlean Luellen, Louisa Balasz, Cynthia Rovnaghi, Robert M. Bryan, Eric E. Lloyd, Elizabeth Fitzpatrick, Jordy S. Saravia, Stephania A. Cormier, Christopher M. Waters

Faculty Publications

Copyright © 2014 by the Society of Critical Care Medicine and Lippincott Williams & Wilkins. Objectives: We previously reported the expression of the twoporedomain K+ channel TREK-1 in lung epithelial cells and proposed a role for this channel in the regulation of alveolar epithelial cytokine secretion. In this study, we focused on investigating the role of TREK-1 in vivo in the development of hyperoxia-induced lung injury. Design: Laboratory animal experiments. Setting: University research laboratory. Subjects: Wild-type and TREK-1-deficient mice. Interventions: Mice were anesthetized and exposed to 1) room air, no mechanical ventilation, 2) 95% hyperoxia for 24 hours, and 3) …

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Response Of Native Insect Communities To Invasive Plants, T. Martijn Bezemer, Jeffrey A. Harvey, James T. Cronin Jan 2014

Response Of Native Insect Communities To Invasive Plants, T. Martijn Bezemer, Jeffrey A. Harvey, James T. Cronin

Faculty Publications

Invasive plants can disrupt a range of trophic interactions in native communities. As a novel resource they can affect the performance of native insect herbivores and their natural enemies such as parasitoids and predators, and this can lead to host shifts of these herbivores and natural enemies. Through the release of volatile compounds, and by changing the chemical complexity of the habitat, invasive plants can also affect the behavior of native insects such as herbivores, parasitoids, and pollinators. Studies that compare insects on related native and invasive plants in invaded habitats show that the abundance of insect herbivores is often …

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Relative Importance Of Host Environment, Transmission Potential And Host Phylogeny To The Structure Of Parasite Metacommunities, Tad Dallas, Steven J. Presley Jan 2014

Relative Importance Of Host Environment, Transmission Potential And Host Phylogeny To The Structure Of Parasite Metacommunities, Tad Dallas, Steven J. Presley

Faculty Publications

Identification of mechanisms that shape parasite community and metacommunity structures have important implications to host health, disease transmission, and the understanding of community assembly in general. Using a long-term dataset on parasites from desert rodents, we examined the relative contributions of host traits that represent important aspects of parasite environment, transmission probability between host species, and host phylogeny to the structure of a parasite metacommunity as well as for taxonomically restricted parasite metacommunities (coccidians, ectoparasites and helminths). This was done using a combination of metacommunity analysis and variance partitioning based on canonical correspondence analysis. Coccidian and ectoparasite metacommunities did not …

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Iron And Zinc Binding Activity Of Escherichia Coli Topoisomerase I Homolog Yrdd, Zishuo Cheng, Guoqiang Tan, Wu Wang, Xiaolu Su, Aaron P. Landry, Jianxin Lu, Huangen Ding Jan 2014

Iron And Zinc Binding Activity Of Escherichia Coli Topoisomerase I Homolog Yrdd, Zishuo Cheng, Guoqiang Tan, Wu Wang, Xiaolu Su, Aaron P. Landry, Jianxin Lu, Huangen Ding

Faculty Publications

YrdD, a homolog of the C-terminal zinc-binding region of Escherichia coli topoisomerase I, is highly conserved among proteobacteria and enterobacteria. However, the function of YrdD remains elusive. Here we report that YrdD purified from E. coli cells grown in LB media contains both zinc and iron. Supplement of exogenous zinc in the medium abolishes the iron binding of YrdD in E. coli cells, indicating that iron and zinc may compete for the same metal binding sites in the protein. While the zinc-bound YrdD is able to bind single-stranded (ss) DNA and protect ssDNA from the DNase I digestion in vitro, …

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Sexual Differences Of Imprinted Genes' Expression Levels, Mohammad Faisal, Hana Kim, Joomyeong Kim Jan 2014

Sexual Differences Of Imprinted Genes' Expression Levels, Mohammad Faisal, Hana Kim, Joomyeong Kim

Faculty Publications

In mammals, genomic imprinting has evolved as a dosage-controlling mechanism for a subset of genes that play critical roles in their unusual reproduction scheme involving viviparity and placentation. As such, many imprinted genes are highly expressed in sex-specific reproductive organs. In the current study, we sought to test whether imprinted genes are differentially expressed between the two sexes. According to the results, the expression levels of the following genes differ between the two sexes of mice: Peg3, Zim1, Igf2, H19 and Zac1. The expression levels of these imprinted genes are usually greater in males than in females. This bias is …

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Thermomicrobium Carboxidum Sp. Nov., And Thermorudis Peleae Gen. Nov., Sp. Nov., Carbon Monoxide-Oxidizing Bacteria Isolated From Geothermally Heated Biofilms, C. E. King, G. M. King Jan 2014

Thermomicrobium Carboxidum Sp. Nov., And Thermorudis Peleae Gen. Nov., Sp. Nov., Carbon Monoxide-Oxidizing Bacteria Isolated From Geothermally Heated Biofilms, C. E. King, G. M. King

Faculty Publications

Two thermophilic, Gram-stain-positive, rod-shaped, non-spore-forming bacteria (strains KI3T and KI4T) were isolated from geothermally heated biofilms growing on a tumulus in the Kilauea Iki pit crater on the flank of Kilauea Volcano (Hawai'i, USA). Strain KI3T grew over an examined temperature range of 50-70 °C (no growth at 80 °C) and a pH range of 6.0-9.0, with optimum growth at 70 °C and pH 7.0. Strain KI4T grew at temperatures of 55-70 °C and a pH range of 5.8-8.0, with optimum growth at 65 °C and pH 6.7-7.1. The DNA G+C contents of strains KI3T and KI4T were 66.0 and …

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Description Of Thermogemmatispora Carboxidivorans Sp. Nov., A Carbon-Monoxideoxidizing Member Of The Class Ktedonobacteria Isolated From A Geothermally Heated Biofilm, And Analysis Of Carbon Monoxide Oxidation By Members Of The Class Ktedonobacteria, C. E. King, G. M. King Jan 2014

Description Of Thermogemmatispora Carboxidivorans Sp. Nov., A Carbon-Monoxideoxidizing Member Of The Class Ktedonobacteria Isolated From A Geothermally Heated Biofilm, And Analysis Of Carbon Monoxide Oxidation By Members Of The Class Ktedonobacteria, C. E. King, G. M. King

Faculty Publications

A thermophilic, aerobic, Gram-stain-positive bacterium (strain PM5T), which formed mycelia of irregularly branched filaments and produced multiple exospores per cell, was isolated from a geothermally heated biofilm. Strain PM5T grew at 40-65 °C and pH 4.1-8.0, with optimal growth at 55 °C and pH 6.0. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain PM5T belonged to the class Ktedonobacteria, and was related most closely to Thermogemmatispora onikobensis ONI-1T (97.7 % similarity) and Thermogemmatispora foliorum ONI-5T (96.1 %). Morphological features and fatty acid profiles (major fatty acids: iso-C17: 0, iso-C19: 0 and 12,17-dimethyl C18: 0) supported the affiliation …

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Urban Microbiomes And Urban Ecology: How Do Microbes In The Built Environment Affect Human Sustainability In Cities?, Gary M. King Jan 2014

Urban Microbiomes And Urban Ecology: How Do Microbes In The Built Environment Affect Human Sustainability In Cities?, Gary M. King

Faculty Publications

© 2014, The Microbiological Society of Korea and Springer-Verlag Berlin Heidelberg. Humans increasingly occupy cities. Globally, about 50% of the total human population lives in urban environments, and in spite of some trends for deurbanization, the transition from rural to urban life is expected to accelerate in the future, especially in developing nations and regions. The Republic of Korea, for example, has witnessed a dramatic rise in its urban population, which now accounts for nearly 90% of all residents; the increase from about 29% in 1955 has been attributed to multiple factors, but has clearly been driven by extraordinary growth …

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Historical And Recent Processes Shaping The Geographic Range Of A Rocky Intertidal Gastropod: Phylogeography, Ecology, And Habitat Availability, Phillip B. Fenberg, Karine Posbic, Michael E. Hellberg Jan 2014

Historical And Recent Processes Shaping The Geographic Range Of A Rocky Intertidal Gastropod: Phylogeography, Ecology, And Habitat Availability, Phillip B. Fenberg, Karine Posbic, Michael E. Hellberg

Faculty Publications

Factors shaping the geographic range of a species can be identified when phylogeographic patterns are combined with data on contemporary and historical geographic distribution, range-wide abundance, habitat/food availability, and through comparisons with codistributed taxa. Here, we evaluate range dynamism and phylogeography of the rocky intertidal gastropod Mexacanthina lugubris lugubris across its geographic range - the Pacific coast of the Baja peninsula and southern California. We sequenced mitochondrial DNA (CO1) from ten populations and compliment these data with museum records, habitat availability and range-wide field surveys of the distribution and abundance of M. l. lugubris and its primary prey (the barnacle …

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Genetic Species Delineation Among Branching Caribbean Porites Corals, C. Prada, M. B. De Biasse, J. E. Neigel, B. Yednock, J. L. Stake, Z. H. Forsman, I. B. Baums, M. E. Hellberg Jan 2014

Genetic Species Delineation Among Branching Caribbean Porites Corals, C. Prada, M. B. De Biasse, J. E. Neigel, B. Yednock, J. L. Stake, Z. H. Forsman, I. B. Baums, M. E. Hellberg

Faculty Publications

© 2014, Springer-Verlag Berlin Heidelberg. Coral species are difficult to discern because of their morphological plasticity, long generation times, and slow rates of mitochondrial DNA evolution. Among Caribbean representatives of the genus Porites are three named species (P. divaricata, P. furcata, and P. porites) with branching colony morphologies whose validity as genetically isolated species has been debated. We present sequence data from the mitochondrial control region, nuclear ITS, and nine single-copy nuclear loci for the Caribbean Porites and a related eastern Pacific species. mtDNA sequences were nearly invariant among the three branching species and their crustose sister P. branneri, and …

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Cryptic Diversity Hides Host And Habitat Specialization In A Gorgonian-Algal Symbiosis, Carlos Prada, Shelby E. Mcilroy, Diana M. Beltrán, Daniel J. Valint, Scott A. Ford, Michael E. Hellberg, Mary Alice Coffroth Jan 2014

Cryptic Diversity Hides Host And Habitat Specialization In A Gorgonian-Algal Symbiosis, Carlos Prada, Shelby E. Mcilroy, Diana M. Beltrán, Daniel J. Valint, Scott A. Ford, Michael E. Hellberg, Mary Alice Coffroth

Faculty Publications

Shallow water anthozoans, the major builders of modern coral reefs, enhance their metabolic and calcification rates with algal symbionts. Controversy exists over whether these anthozoan-algae associations are flexible over the lifetimes of individual hosts, promoting acclimative plasticity, or are closely linked, such that hosts and symbionts co-evolve across generations. Given the diversity of algal symbionts and the morphological plasticity of many host species, cryptic variation within either partner could potentially confound studies of anthozoan-algal associations. Here, we used ribosomal, organelle and nuclear sequences, along with microsatellite variation, to study the relationship between lineages of a common Caribbean gorgonian and its …

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Optimized Inhibitors Of Soluble Epoxide Hydrolase Improve In Vitro Target Residence Time And In Vivo Efficacy, Kin Sing Stephen Lee, Jun Yan Liu, Karen M. Wagner, Svetlana Pakhomova, Hua Dong, Christophe Morisseau, Samuel H. Fu, Jun Yang, Peng Wang, Arzu Ulu, Christina A. Mate, Long V. Nguyen, Sung Hee Hwang, Matthew L. Edin, Alexandria A. Mara, Heike Wulff, Marcia E. Newcomer, Darryl C. Zeldin, Bruce D. Hammock Jan 2014

Optimized Inhibitors Of Soluble Epoxide Hydrolase Improve In Vitro Target Residence Time And In Vivo Efficacy, Kin Sing Stephen Lee, Jun Yan Liu, Karen M. Wagner, Svetlana Pakhomova, Hua Dong, Christophe Morisseau, Samuel H. Fu, Jun Yang, Peng Wang, Arzu Ulu, Christina A. Mate, Long V. Nguyen, Sung Hee Hwang, Matthew L. Edin, Alexandria A. Mara, Heike Wulff, Marcia E. Newcomer, Darryl C. Zeldin, Bruce D. Hammock

Faculty Publications

Diabetes is affecting the life of millions of people. A large proportion of diabetic patients suffer from severe complications such as neuropathic pain, and current treatments for these complications have deleterious side effects. Thus, alternate therapeutic strategies are needed. Recently, the elevation of epoxy-fatty acids through inhibition of soluble epoxide hydrolase (sEH) was shown to reduce diabetic neuropathic pain in rodents. In this report, we describe a series of newly synthesized sEH inhibitors with at least 5-fold higher potency and doubled residence time inside both the human and rodent sEH enzyme than previously reported inhibitors. These inhibitors also have better …

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Phylogeny, Classification, And Fruit Evolution Of The Species-Rich Neotropical Bellflowers (Campanulaceae: Lobelioideae), Laura P. Lagomarsino, Alexandre Antonelli, Nathan Muchhala, Allan Timmermann, Sarah Mathews, Charles C. Davis Jan 2014

Phylogeny, Classification, And Fruit Evolution Of The Species-Rich Neotropical Bellflowers (Campanulaceae: Lobelioideae), Laura P. Lagomarsino, Alexandre Antonelli, Nathan Muchhala, Allan Timmermann, Sarah Mathews, Charles C. Davis

Faculty Publications

© 2014 Botanical Society of America. Premise of the study: The species-rich Neotropical genera Centropogon, Burmeistera, and Siphocampylus represent more than half of the ∼1200 species in the subfamily Lobelioideae (Campanulaceae). They exhibit remarkable morphological variation in floral morphology and habit. Limited taxon sampling and phylogenetic resolution, however, obscures our understanding of relationships between and within these genera and underscores our uncertainty of the systematic value of fruit type as a major diagnostic character.Methods: We inferred a phylogeny from five plastid DNA regions (rpl32-trnL, ndhF-rpl32, rps16-trnK, trnG-trnG-trns, rbcL) using maximum-likelihood and Bayesian inference. Ancestral character reconstructions were applied to infer …

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Pleading Patterns And The Role Of Litigation As A Driver Of Federal Climate Change Legislation, Juscelino F. Colares, Kosta Ristovski Jan 2014

Pleading Patterns And The Role Of Litigation As A Driver Of Federal Climate Change Legislation, Juscelino F. Colares, Kosta Ristovski

Faculty Publications

Based on a variant of the Elliott-Ackerman-Millian theory that variable, potentially inconsistent and costly litigation outcomes induce industry to seek federal preemptive legislation to reign in such costs, we collect data on climate change-related litigation to determine whether litigation might motivate major greenhouse gas emitters to accept a preemptive, though possibly carbon-restricting, legislative compromise. We conduct a spectral cluster analysis on 178 initial federal and state judicial filings to reveal the most relevant groupings among climate change-related suits and their underlying pleading patterns. Besides exposing the general content and structure of climate change-related filings, this study identifies major specific pleading …

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Busting Myths About ‘Species’, Charles H. Pence Jan 2014

Busting Myths About ‘Species’, Charles H. Pence

Faculty Publications

No abstract provided.

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African Arrivals And Transformations, Andrew Sluyter Jan 2014

African Arrivals And Transformations, Andrew Sluyter

Faculty Publications

No abstract provided.

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Biodistribution Of Plga And Plga/Chitosan Nanoparticles After Repeat-Dose Oral Delivery In F344 Rats For 7 Days, Sara M. Navarro, Caleb Darensbourg, Linda Cross, Rhett Stout, Diana Coulon, Carlos E. Astete, Timothy Morgan, Cristina M. Sabliov Jan 2014

Biodistribution Of Plga And Plga/Chitosan Nanoparticles After Repeat-Dose Oral Delivery In F344 Rats For 7 Days, Sara M. Navarro, Caleb Darensbourg, Linda Cross, Rhett Stout, Diana Coulon, Carlos E. Astete, Timothy Morgan, Cristina M. Sabliov

Faculty Publications

© 2014 Future Science Ltd. Aim: To quantify in vivo the biodistribution of poly(lactic-co-glycolic) acid (PLGA) and PLGA/chitosan nanoparticles (PLGA/Chi NPs) and assess if the positive charge of chitosan significantly enhances nanoparticle absorption in the GI tract.Material & methods: PLGA and PLGA/Chi NPs covalently linked to tetramethylrhodamine-5-isothiocyanate (TRITC) were orally administered to F344 rats for 7 days, and the biodistribution of fluorescent NPs was analyzed in different organs.Results: The highest amount of particles (% total dose/g) was detected for both treatments in the spleen, followed by intestine and kidney, and then by liver, lung, heart and brain, with no significant …

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Biodistribution Of Plga And Plga/Chitosan Nanoparticles After Repeat-Dose Oral Delivery In F344 Rats For 7 Days, Sara M. Navarro, Caleb Darensbourg, Linda Cross, Rhett Stout, Diana Coulon, Carlos E. Astete, Timothy Morgan, Cristina M. Sabliov Jan 2014

Biodistribution Of Plga And Plga/Chitosan Nanoparticles After Repeat-Dose Oral Delivery In F344 Rats For 7 Days, Sara M. Navarro, Caleb Darensbourg, Linda Cross, Rhett Stout, Diana Coulon, Carlos E. Astete, Timothy Morgan, Cristina M. Sabliov

Faculty Publications

© 2014 Future Science Ltd. Aim: To quantify in vivo the biodistribution of poly(lactic-co-glycolic) acid (PLGA) and PLGA/chitosan nanoparticles (PLGA/Chi NPs) and assess if the positive charge of chitosan significantly enhances nanoparticle absorption in the GI tract.Material & methods: PLGA and PLGA/Chi NPs covalently linked to tetramethylrhodamine-5-isothiocyanate (TRITC) were orally administered to F344 rats for 7 days, and the biodistribution of fluorescent NPs was analyzed in different organs.Results: The highest amount of particles (% total dose/g) was detected for both treatments in the spleen, followed by intestine and kidney, and then by liver, lung, heart and brain, with no significant …

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Ruminant Self-Medication Against Gastrointestinal Nematodes: Evidence, Mechanism, And Origins Ẫ†, Juan J. Villalba, James Miller, Eugene D. Ungar, Serge Y. Landau, John Glendinning Jan 2014

Ruminant Self-Medication Against Gastrointestinal Nematodes: Evidence, Mechanism, And Origins Ẫ†, Juan J. Villalba, James Miller, Eugene D. Ungar, Serge Y. Landau, John Glendinning

Faculty Publications

Gastrointestinal helminths challenge ruminants in ways that reduce their fitness. In turn, ruminants have evolved physiological and behavioral adaptations that counteract this challenge. Ruminants display anorexia and avoidance behaviors, which tend to reduce the incidence of parasitism. In addition, ruminants appear to learn to self-medicate against gastrointestinal parasites by increasing consumption of plant secondary compounds with antiparasitic actions. This selective feeding improves health and fitness. Here, we review the evidence for self-medication in ruminants, propose a hypothesis to explain self-medicative behaviors (based on post-ingestive consequences), and discuss mechanisms (e.g., enhanced neophilia, social transmission) that may underlie the ontogeny and spread …

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Group 3 Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana: Structural Properties And Protective Abilities During Desiccation, Leaf C. Boswell, Michael A. Menze, Steven C. Hand Jan 2014

Group 3 Late Embryogenesis Abundant Proteins From Embryos Of Artemia Franciscana: Structural Properties And Protective Abilities During Desiccation, Leaf C. Boswell, Michael A. Menze, Steven C. Hand

Faculty Publications

© 2014 by The University of Chicago. All rights reserved. Group 3 late embryogenesis abundant (LEA) proteins are highly hydrophilic, and their expression is associated with desiccation tolerance in both plants and animals. Here we show that two LEA proteins from embryos of Artemia franciscana, AfrLEA2 and AfrLEA3m, are intrinsically disordered in solution but upon desiccation gain secondary structure, as measured by circular dichroism. Trifluoroethanol and sodium dodecyl sulfate are both shown to induce a-helical structure in AfrLEA2 and AfrLEA3m. Bioinformatic predictions of secondary-structure content for both proteins correspond most closely to conformations measured in the dry state. Because some …

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Quantification Of Cellular Protein Expression And Molecular Features Of Group 3 Lea Proteins From Embryos Of Artemia Franciscana, Leaf C. Boswell, Daniel S. Moore, Steven C. Hand Jan 2014

Quantification Of Cellular Protein Expression And Molecular Features Of Group 3 Lea Proteins From Embryos Of Artemia Franciscana, Leaf C. Boswell, Daniel S. Moore, Steven C. Hand

Faculty Publications

Late embryogenesis abundant (LEA) proteins are highly hydrophilic, low complexity proteins whose expression has been correlated with desiccation tolerance in anhydrobiotic organisms. Here, we report the identification of three new mitochondrial LEA proteins in anhydrobiotic embryos of Artemia franciscana, AfrLEA3m-47, AfrLEA3m-43, and AfrLEA3m-29. These new isoforms are recognized by antibody raised against recombinant AfrLEA3m, the original mitochondrial-targeted LEA protein previously reported from these embryos; mass spectrometry confirms all four proteins share sequence similarity. The corresponding messenger RNA (mRNA) species for the four proteins are readily amplified from total complementary DNA (cDNA) prepared from embryos. cDNA sequences of the four mRNAs …

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Modulation Of Vacuolar Ph Is Required For Replication Of Edwardsiella Ictaluri In Channel Catfish Macrophages, Wes A. Baumgartner, Lidiya Dubytska, Matthew L. Rogge, Peter J. Mottram, Ronald L. Thune Jan 2014

Modulation Of Vacuolar Ph Is Required For Replication Of Edwardsiella Ictaluri In Channel Catfish Macrophages, Wes A. Baumgartner, Lidiya Dubytska, Matthew L. Rogge, Peter J. Mottram, Ronald L. Thune

Faculty Publications

Previous in vitro work demonstrated that Edwardsiella ictaluri produces an acid-activated urease that can modulate environmental pH through the production of ammonia from urea. Additional work revealed that expression of the E. ictaluri type III secretion system (T3SS) is upregulated by acidic pH. Both the urease and the T3SS were previously shown to be essential to intracellular replication. In this work, fluorescence microscopy with LysoTracker Red DND-99 (LTR) indicated that E. ictaluri-containing vacuoles (ECV) became acidified following ingestion by head kidney-derived macrophages (HKDM). In vivo ratiometric imaging demonstrated a lowered ECV pH, which fell to as low as pH 4 …

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Habitat Suitability Under Changing Climatic Conditions For The Exotic Ambrosia Beetle, Cnestus Mutilatus (Curculionidae: Scolytinae: Xyleborini) In The Southeastern United States, Rabiu Olatinwo, Douglas Streett, Christopher Carlton Jan 2014

Habitat Suitability Under Changing Climatic Conditions For The Exotic Ambrosia Beetle, Cnestus Mutilatus (Curculionidae: Scolytinae: Xyleborini) In The Southeastern United States, Rabiu Olatinwo, Douglas Streett, Christopher Carlton

Faculty Publications

The camphor shot borer, Cnestus mutilatus (Blandford) is a nonnative ambrosia beetle first reported in the United States in 1999 at Oktibbeha County in Mississippi. Although, C. mutilatus is a major pest of several trees in its native habitat in Asia, it is not yet a major pest in the United States. However, the range expansion in recent years across the southeastern region indicates that C. mutilatus could be adapting quickly to the new environment, perhaps because of the availability of numerous host trees and suitable environmental conditions that support the population. As the population increases, future outbreaks of C. …

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Host Range Tests Reveal Paectes Longiformis Is Not A Suitable Biological Control Agent For The Invasive Plant Schinus Terebinthifolia, Veronica Manrique, Rodrigo Diaz, Todd Condon, W. A. Overholt Jan 2014

Host Range Tests Reveal Paectes Longiformis Is Not A Suitable Biological Control Agent For The Invasive Plant Schinus Terebinthifolia, Veronica Manrique, Rodrigo Diaz, Todd Condon, W. A. Overholt

Faculty Publications

© 2014, International Organization for Biological Control (IOBC). The most critical step during a weed biological control program is determination of a candidate agent’s host range. Despite rigorous protocols and extensive testing, there are still concerns over potential non-target effects following field releases. With the objective to improve risk assessment in biological control, no-choice and choice testing followed by a multiple generation study were conducted on the leaf-defoliator, Paectes longiformis Pogue (Lepidoptera: Euteliidae). This moth is being investigated as a biological control agent of Schinus terebinthifolia Raddi (Sapindales: Anacardiaceae), which is one of the worst invasive plant species in Florida, …

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Temperature-Dependent Development, Cold Tolerance, And Potential Distribution Of Cricotopus Lebetis (Diptera: Chironomidae), A Tip Miner Of Hydrilla Verticillata (Hydrocharitaceae), Karen N. Stratman, William A. Overholt, James P. Cuda, A. Mukherjee, R. Diaz, Michael D. Netherland, Patrick C. Wilson Jan 2014

Temperature-Dependent Development, Cold Tolerance, And Potential Distribution Of Cricotopus Lebetis (Diptera: Chironomidae), A Tip Miner Of Hydrilla Verticillata (Hydrocharitaceae), Karen N. Stratman, William A. Overholt, James P. Cuda, A. Mukherjee, R. Diaz, Michael D. Netherland, Patrick C. Wilson

Faculty Publications

© The Author 2014. Published by Oxford University Press on behalf of the Entomological Society of America. A chironomid midge, Cricotopus lebetis (Sublette) (Diptera: Chironomidae), was discovered attacking the apical meristems of Hydrilla verticillata (L.f. Royle) in Crystal River, Citrus Co., Florida in 1992. The larvae mine the stems of H. verticillata and cause basal branching and stunting of the plant. Temperature-dependent development, cold tolerance, and the potential distribution of the midge were investigated. The results of the temperature-dependent development study showed that optimal temperatures for larval development were between 20 and 30°C, and these data were used to construct …

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Differential Effects Of Akt Isoforms On Somatic Cell Reprogramming, Yong Tang, Zongliang Jiang, Yan Luo, Xueming Zhao, Ling Wang, Carol Norris, Xiuchun Cindy Tian Jan 2014

Differential Effects Of Akt Isoforms On Somatic Cell Reprogramming, Yong Tang, Zongliang Jiang, Yan Luo, Xueming Zhao, Ling Wang, Carol Norris, Xiuchun Cindy Tian

Faculty Publications

© 2014. Akt plays an important role in cell growth, proliferation and survival. The specific roles of the three Akt isoforms in somatic cell reprogramming have not been investigated. Here we report that, during iPSC generation, enhanced Akt1 activity promotes complete reprogramming mainly through increased activation of Stat3 in concert with leukemia inhibitory factor (LIF) and, to a lesser extent, through promotion of colony formation. Akt1 augments Stat3 activity through activation of mTOR and upregulation of LIF receptor expression. Similarly, enhanced Akt2 or Akt3 activation also promotes reprogramming and coordinates with LIF to activate Stat3. Blocking Akt1 or Akt3 but …

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Development Of Microsatellites For Population Genetic Analyses Of Tabanus Nigrovittatus (Diptera: Tabanidae), Claudia Husseneder, Jennifer R. Delatte, Jeremy Krumholt, Lane D. Foil Jan 2014

Development Of Microsatellites For Population Genetic Analyses Of Tabanus Nigrovittatus (Diptera: Tabanidae), Claudia Husseneder, Jennifer R. Delatte, Jeremy Krumholt, Lane D. Foil

Faculty Publications

ABSTRACT The greenhead horse fly, Tabanus nigrovittatus Macquart (Diptera: Tabanidae), is frequently found in coastal marshes of the Eastern United States. The females are autogenous (i.e., able to develop eggs without a bloodmeal), but they become a considerable pest to both humans and animals when they pursue a source of blood protein to produce additional eggs. In this study, we identified microsatellite markers to provide first insight into the population genetic structure of this notorious pest species. Because no prior genomic information was available for T. nigrovittatus,weused direct shotgun pyrosequencing technology to characterize microsatellite loci. Approximately 10% of the 105,634 …

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