Digital Commons Network^™

Spatial Pattern And Differential Expression Analysis With Spatial Transcriptomic Data, Fei Qin, Xizhi Luo, Bo Cai Ph.D., Feifei Xiao, Guoshuai Cai

Faculty Publications

The emergence of spatial transcriptomic technologies has opened new avenues for investigating gene activities while preserving the spatial context of tissues. Utilizing data generated by such technologies, the identification of spatially variable (SV) genes is an essential step in exploring tissue landscapes and biological processes. Particularly in typical experimental designs, such as case-control or longitudinal studies, identifying SV genes between groups is crucial for discovering significant biomarkers or developing targeted therapies for diseases. However, current methods available for analyzing spatial transcriptomic data are still in their infancy, and none of the existing methods are capable of identifying SV genes between …

A New Synthesis Of Arctic-Boreal Carbon Fluxes For Improved Carbon Budget Estimates, Anna Virkkala, Isabel Wargowsky, Judith Vogt, Mckenzie Kuhn, Susan Natali, Brendan Rogers, Mathias Goeckede, Kyle Arndt, Jennifer Watts, Tiffany Windholz, Simran Madaan

Faculty Publications

The Arctic-boreal zone and its permafrost regions have historically been sparsely measured for carbon dioxide and methane fluxes. This data sparsity has created significant uncertainties in Arctic-boreal carbon budget estimates. However, over the past decade, the availability of Arctic-boreal carbon flux data has increased substantially. Yet, it remains scattered across different repositories, papers, and unpublished sources, making it hard to estimate more accurate Arctic-boreal carbon budgets. To address this research gap, we have compiled a database of Arctic-boreal carbon fluxes (ABCFlux v2) from flux repositories, literature, and site principal investigators, which will be openly distributed. The database includes carbon dioxide …

Implications Of Energetic Neutral Atoms Observed With Ibex-Lo For The Pressure Balance In The Heliosphere, André Galli, Peter Wurz, Nathan A. Schwadron, Eberhard Möbius, Stephen A. Fuselier, Justyna M. Sokół, Maciej Bzowski, Paweł Swaczyna, Kostas Dialynas, David J. Mccomas

Faculty Publications

Energetic Neutral Atoms (ENAs) from the heliosphere are a unique means to remotely image the boundary regions of our heliosphere. NASA's Interstellar Boundary Explorer (IBEX) has been very successful in measuring these ENAs since 2008 at energies from tens of eV to 6 keV.The ENA low energy range from tens of eV to solar wind energy (roughly 1 keV) has been sampled throughout an entire solar cycle with the IBEX-Lo ENA imager. This enables us to study the physical implications for the structure of the heliosphere and for the parent proton populations in the heliosheath and other plasma regions that …

Motivational Dysregulation With Melanocortin 4 Receptor Haploinsufficiency, Alex M. Steiner, Robert F. Roscoe, Rosemarie M. Booze, Charles F. Mactutus

Faculty Publications

Obesity, by any standard, is a global health crisis. Both genetic and dietary contributions to the development and maintenance of obesity were integral factors of our experimental design. As mutations of the melanocortin 4 receptors (MC4R) are the leading monogenetic cause of obesity, MC4R haploinsufficient rats were fed a range of dietary fat (0–12 %) in a longitudinal design. Physiological and motivational assessments were performed using a locomotor task, a 5-choice sucrose preference task, an operant task with fixed and progressive ratios, as well as a distraction operant task. Dendritic spine morphology of medium spiny neurons (MSNs) of the nucleus …

Sound Innovations For Biofabrication And Tissue Engineering, Mengxi Wu, Zhiteng Ma, Zhenhua Tian, Joseph Rich, Xin He, Jianping Xia, Ye He, Kaichun Yang, Shujie Yang, Kam W. Leong, Luke P. Lee, Tony Jun Huang

Faculty Publications

Advanced biofabrication techniques can create tissue-like constructs that can be applied for reconstructive surgery or as in vitro three-dimensional (3D) models for disease modeling and drug screening. While various biofabrication techniques have recently been widely reviewed in the literature, acoustics-based technologies still need to be explored. The rapidly increasing number of publications in the past two decades exploring the application of acoustic technologies highlights the tremendous potential of these technologies. In this review, we contend that acoustics-based methods can address many limitations inherent in other biofabrication techniques due to their unique advantages: noncontact manipulation, biocompatibility, deep tissue penetrability, versatility, precision …

Strategies To Alleviate Flickering: Bayesian And Smoothing Methods For Deep Learning Classification In Video, Noah Miller, Glen Ryan Drumm, Lance Champagne, Bruce A. Cox, Trevor Bihl

Faculty Publications

Excerpt: Increasing reliance on autonomous systems requires confidence in the accuracies produced from computer vision classification algorithms. Computer vision (CV) for video classification provides phenomenal abilities, but it often suffers from “flickering” of results. Flickering occurs when the CV algorithm switches between declared classes over successive frames. Such behavior causes a loss of trust and confidence in their operations.

Exploring In-Service Science Teachers' Self-Perceptions Of Competence And Pedagogical Approaches To Socioscientific Issues In Education, Arberore Bicaj, Fatlume Berisha, Rachel Gisewhite

Faculty Publications

Global education experts are increasingly prioritizing scientific literacy development. Science education is key to enhancing scientific literacy, fostering social responsibility, and supporting economic growth by equipping individuals with essential scientific knowledge and skills. In the past two decades, socioscientific issues (SSI) have greatly influenced science education research, improving students’ ability to apply scientific knowledge in real-world contexts and advancing functional scientific literacy. Given the strong evidence that socioscientific issues (SSI) enhance scientific literacy and social responsibility skills, understanding SSI role in science education is crucial for developing countries aiming to boost economic and social growth. This research aimed to understand …

The Suppression Of Ocean Waves By Biogenic Slicks, Nathan J. M. Laxague, Christopher Zappa, Shantanu Soumya, Oliver Wurl

Faculty Publications

Surface waves are significantly damped by biogenic surfactants which accumulate at the ocean's surface from the high latitudes to the tropics. The growth, development, and breaking of short wind-driven surface waves is a key mediator of the air-sea exchange of momentum, heat, and trace gases consequently, the effect of surfactant compounds in the uppermost millimeters of the ocean in damping short surface waves bears on earth's climatic processes. The mechanisms through which surfactants suppress waves have been studied in great detail through careful laboratory experimentation in quasi one-dimensional wave tanks. However, the spatial scales over which this damping occurs in …

From "Open Ocean" To "Exposed Aquaculture": Why And How We Are Changing The Standard Terminology Describing "Offshore Aquaculture", Tyler Sclodnick, Michael Chambers, Barry Antonio Costa-Pierce, Tobias Dewhurst, Nils Goseberg, Kevin Gerald Heasman, Wolf Isbert, Gesche Krause, Daniel Wieczorek, Till Markus, Bela H. Buck

Faculty Publications

The term “offshore” with regards to aquaculture has hitherto encompassed various perspectives, including technology, geographic location, legal jurisdiction, and more. To resolve the ambiguity in this term and understand its implications for current and future aquaculture development, “offshore” should be resolved into two separate metrics: distance from shore and energy exposure. The United Nations Convention on the Law of the Sea (UNCLOS) distinguishes between internal waters, territorial sea, contiguous zone, exclusive economic zone (EEZ), and the high seas, but currently has no precise definition for “offshore” in its provisions, and therefore no applicable laws pertaining to “offshore” aquaculture. Regulating a …

From "Open Ocean" To "Exposed Aquaculture": Why And How We Are Changing The Standard Terminology Describing "Offshore Aquaculture", Tyler Sclodnick, Michael Chambers, Barry Antonio Costa-Pierce, Tobias Dewhurst, Nils Goseberg, Kevin Gerald Heasman, Wolf Isbert, Gesche Krause, Daniel Wieczorek, Till Markus, Bela H. Buck

Faculty Publications

The term “offshore” with regards to aquaculture has hitherto encompassed various perspectives, including technology, geographic location, legal jurisdiction, and more. To resolve the ambiguity in this term and understand its implications for current and future aquaculture development, “offshore” should be resolved into two separate metrics: distance from shore and energy exposure. The United Nations Convention on the Law of the Sea (UNCLOS) distinguishes between internal waters, territorial sea, contiguous zone, exclusive economic zone (EEZ), and the high seas, but currently has no precise definition for “offshore” in its provisions, and therefore no applicable laws pertaining to “offshore” aquaculture. Regulating a …

Master Narratives Surrounding Rural Emerging Adult Migration: Resources For Postsecondary Decision-Making In Rural Communities, Cindy Hartman, Jayson Seaman, Andrew D. Coppens, Erin H. Sharp, Sarah Jusseaume

Faculty Publications

This study examined the cultural messages informing postsecondary decision-making processes surrounding emerging adult migration from/to a predominately rural state in the northeastern U.S. Utilizing an ecological approach to sampling, this study used 27 interviews and 5 focus groups of stakeholders working in educational, employment, and quality of life sectors. Narrative analysis yielded evidence of a predominant master narrative of migration as a developmental requirement during emerging adulthood. Stakeholders spoke of normative and prescriptive expectations that emerging adults particularly in rural areas orient their plans and decision-making around, including leaving their home communities for developmental reasons. Implications for emerging adult development …

Single-Molecule Profiling Of Per- And Polyfluoroalkyl Substances By Cyclodextrin Mediated Host-Guest Interactions Within A Biological Nanopore, Xiaojun Wei, Aditya Choudhary, Leon Y. Wang, Lixing Yang, Mark J. Uline, Mario Tagliazucchi, Qian Wang, Dmitry Bedrov, Chang Liu

Faculty Publications

Biological nanopores are increasingly used in molecular sensing due to their single-molecule sensitivity. The detection of per- and polyfluoroalkyl substances (PFAS) like perfluorooctanoic acid and perfluorooctane sulfonic acid is critical due to their environmental prevalence and toxicity. Here, we investigate selective interactions between PFAS and four cyclodextrin (CD) variants (α-, β-, γ-, and 2-hydroxypropyl-γ-CD) within an α-hemolysin nanopore. We demonstrate that PFAS molecules can be electrochemically sensed by interacting with a γ-CD in a nanopore. Using HP-γ-CDs with increased steric resistance, we can identify homologs of the perfluoroalkyl carboxylic acid and the perfluoroalkyl sulfonic acid families and detect common PFAS …

Contrasting Responses Of Commercially Important Northwest Atlantic Bivalve Species To Ocean Acidification And Temperature Conditions, Teagan Mcmahon, Diana Thatcher, Branwen Williams, Alan Wanamaker, Brittany Jellison, Heidi Franklin, Katherine Guay, Nina M. Whitney, Joseph A. Stewart, Michèle Lavigne

Faculty Publications

Modern calcifying marine organisms face numerous environmental stressors, including overfishing, deoxygenation, increasing ocean temperatures, and ocean acidification (OA). Coastal marine settings are predicted to become warmer and more acidic in coming decades, heightening the risks of extreme events such as marine heat waves. Given these threats, it is important to understand the vulnerabilities of marine organisms that construct their shells from calcium carbonate, which are particularly susceptible to warming and decreasing pH levels. To investigate the response of four commercially relevant bivalve species to OA and differing temperatures, juvenile Mercenaria mercenaria (hard shell clams), juvenile Mya arenaria (soft shell clams), …

Contrasting Responses Of Commercially Important Northwest Atlantic Bivalve Species To Ocean Acidification And Temperature Conditions, Teagan Mcmahon, Diana Thatcher, Branwen Williams, Alan Wanamaker, Brittany Jellison, Heidi Franklin, Katherine Guay, Nina M. Whitney, Joseph A. Stewart, Michèle Lavigne

Faculty Publications

Modern calcifying marine organisms face numerous environmental stressors, including overfishing, deoxygenation, increasing ocean temperatures, and ocean acidification (OA). Coastal marine settings are predicted to become warmer and more acidic in coming decades, heightening the risks of extreme events such as marine heat waves. Given these threats, it is important to understand the vulnerabilities of marine organisms that construct their shells from calcium carbonate, which are particularly susceptible to warming and decreasing pH levels. To investigate the response of four commercially relevant bivalve species to OA and differing temperatures, juvenile Mercenaria mercenaria (hard shell clams), juvenile Mya arenaria (soft shell clams), …

Neville Fletcher's Vibrant Valve Voyage, Whitney L. Coyle

Faculty Publications

Fletcher's seminal 1993 paper explores the dynamics of pressure-controlled valves critical in sound production for woodwind and brass instruments and animal vocalizations.1 The study examines four simple valve configurations, determined by the effect of overpressures on valve openings. It identifies conditions under which these valves can sustain self-sustained oscillations, particularly when the ducts connected to the valves are shorter than a quarter of the wavelength at the valve's resonant frequency. The research focuses on the initiation of small amplitude oscillations, setting the stage for understanding larger amplitude nonlinear oscillations.

Integrative Highway Rights-Of-Way Management To Reduce Stormwater Run-Off And Enhance Habitat, Madeline M. Showers, Robin M. Rotman

Faculty Publications

Nature-based management along highway rights-of-way (ROW) has been a focus in several states to benefit pollinators through supporting diverse and native vegetative communities. There is the potential to enhance the ecological benefits of this practice by integrating stormwater management into restoration plans and incorporating other complex, multidisciplinary factors. Historically, lack of technical assistance and limited resources has led managers to use unsustainable roadside practices that reduce the potential ecological services of the ROW; however, integrative management can aid in the prioritization of management efforts and link objectives of various resource agencies. In this literature review, we address the ecological impacts …

Threshold Changes In Winter Temperature And Precipitation Drive Threshold Responses Across Nine Global Climate Zones And Associated Biomes, Alexandra R. Contosta, Kyle A. Arndt, Helen M. Baulch, Nora J. Casson, Adrian Harpold, Toni Lyn Morelli, Alexej P.K. Sirén

Faculty Publications

Globally, winter temperatures are rising, and snowpack is shrinking or disappearing entirely. Despite previous research and published literature reviews, it remains unknown whether biomes across the globe will cross important thresholds in winter temperature and precipitation that will lead to significant ecological changes. Here, we combine the widely used Köppen–Geiger climate classification system with worst-case-scenario projected changes in global monthly temperature and precipitation to illustrate how multiple climatic zones across Earth may experience shifting winter conditions by the end of this century. We then examine how these shifts may affect ecosystems within corresponding biomes. Our analysis demonstrates potential widespread losses …

The Effects Of Anthropogenic Stressors On Above- And Belowground Phytochemical Diversity Of The Wetland Grass, Phragmites Australis, Andrea E. Glassmire, Ana L. Salgado, Rodrigo Diaz, Joseph Johnston, Laura A. Meyerson, Joshua S. Snook, James T. Cronin

Faculty Publications

Coastal wetlands face threats from climate change-induced flooding and biological invasions. Plants respond to these stressors through changes in their phytochemical metabolome, but it is unclear whether stressors affecting one tissue compartment (e.g., leaves) create vulnerabilities in others (e.g., roots) or elicit similar responses across tissues. Additionally, responses to multiple simultaneous stressors remain poorly understood due to the focus on individual metabolites in past studies. This study aims to elucidate how the phytochemical metabolome of three Phragmites australis (Cav.) lineages, common in the Mississippi River Delta, responds to flooding and infestation by the non-native scale insect Nipponaclerda biwakoensis (Kuwana). Among …

Historic Breeding Practices Contribute To Germplasm Divergence In Leaf Specialized Metabolism And Ecophysiology In Cultivated Sunflower (Helianthus Annuus), Jordan A. Dowell, Alan W. Bowsher, Amna Jamshad, Rahul Shah, John M. Burke, Lisa A. Donovan, Chase M. Mason

Faculty Publications

Premise: The use of hybrid breeding systems to increase crop yields has been the cornerstone of modern agriculture and is exemplified in the breeding and improvement of cultivated sunflower (Helianthus annuus). However, it is poorly understood what effect supporting separate breeding pools in such systems, combined with continued selection for yield, may have on leaf ecophysiology and specialized metabolite variation. Methods: We analyzed 288 lines of cultivated H. annuus to examine the genomic basis of several specialized metabolites and agronomically important traits across major heterotic groups. Results: Heterotic group identity supports phenotypic divergences between fertility restoring and cytoplasmic male-sterility maintainer …

Combined Toxicity Of Multi-Walled Carbon Nanotubes And Cu2+ On The Growth Of Ryegrass: Effect Of Surface Modification, Dose, And Exposure Time Pattern, Wenwen Xie, Cheng Peng, Weiping Wang, Xiaoyi Chen, Jiaqi Tan, Wei Zhang

Faculty Publications

The escalating release of multi-walled carbon nanotubes (MWCNTs) into the environment has raised concerns due to their potential ecotoxicological impacts. However, their combined phytotoxicity with heavy metals such as copper (Cu) is still unclear. This study investigated the individual and combined toxic effects of MWCNTs (MWCNT, MWCNT-OH, and MWCNT-COOH) and Cu²⁺ on ryegrass (Lolium multiflorum), uniquely considering different addition orders. The results show that Cu severely inhibited the growth of ryegrass while MWCNTs exhibited a hormesis effect on ryegrass. When MWCNT and Cu were combined, the malondialdehyde (MDA) content in ryegrass showed a 32.39% increase at 20 mg/L MWCNT …

Venomcap: An Exon-Capture Probe Set For The Targeted Sequencing Of Snake Venom Genes, Scott L. Travers, Carl R. Hutter, Christopher C. Austin, Stephen C. Donnellan, Matthew D. Buehler, Christopher E. Ellison, Sara Ruane

Faculty Publications

Snake venoms are complex mixtures of toxic proteins that hold significant medical, pharmacological and evolutionary interest. To better understand the genetic diversity underlying snake venoms, we developed VenomCap, a novel exon-capture probe set targeting toxin-coding genes from a wide range of elapid snakes, with a particular focus on the ecologically diverse and medically important subfamily Hydrophiinae. We tested the capture success of VenomCap across 24 species, representing all major elapid lineages. We included snake phylogenomic probes in the VenomCap capture set, allowing us to compare capture performance between venom and phylogenomic loci and to infer elapid phylogenetic relationships. We demonstrated …

Deephive: A Reinforcement Learning Approach For Automated Discovery Of Swarm-Based Optimization Policies, Eloghosa Ikponmwoba, Opeoluwa Owoyele

Faculty Publications

We present an approach for designing swarm-based optimizers for the global optimization of expensive black-box functions. In the proposed approach, the problem of finding efficient optimizers is framed as a reinforcement learning problem, where the goal is to find optimization policies that require a few function evaluations to converge to the global optimum. The state of each particle within the swarm is defined as its current position and function value within a design space, and the particles learn to take favorable actions that maximize the reward, which is based on the final value of the objective function. The proposed approach …

Research Gaps And Priorities For Quantitative Microbial Risk Assessment (Qmra), Kerry A. Hamilton, Joanna Ciol Harrison, Jade Mitchell, Mark Weir, Marc Verhougstraete, Charles N. Haas, A. Pouyan Nejadhashemi, Julie Libarkin, Tiong Gim Aw, Kyle Bibby, Aaron Bivins, Joe Brown, Kara Dean, Gwyneth Dunbar, Joseph N.S. Eisenberg, Monica Emelko, Daniel Gerrity, Patrick L. Gurian, Emma Hartnett, Michael Jahne, Rachael M. Jones, Timothy R. Julian, Hongwan Li, Yanbin Li, Jacqueline Mac Donald Gibson, Gertjan Medema, J. Scott Meschke, Alexis Mraz, Heather Murphy, David Oryang, Emmanuel De Graft Johnson Owusu-Ansah, Emily Pasek, Abani K. Pradhan

Faculty Publications

The coronavirus disease 2019 pandemic highlighted the need for more rapid and routine application of modeling approaches such as quantitative microbial risk assessment (QMRA) for protecting public health. QMRA is a transdisciplinary science dedicated to understanding, predicting, and mitigating infectious disease risks. To better equip QMRA researchers to inform policy and public health management, an Advances in Research for QMRA workshop was held to synthesize a path forward for QMRA research. We summarize insights from 41 QMRA researchers and experts to clarify the role of QMRA in risk analysis by (1) identifying key research needs, (2) highlighting emerging applications of …

Personhood, Politics, Assisted Reproduction, And The Law Post-Dobbs, Myrisha S. Lewis

Faculty Publications

With the end of the federal constitutional right to abortion, state legislatures and state courts have become far more important in the sphere of reproductive rights. Before Dobbs, anti-abortion advocates had significant (albeit increasingly porous) ceilings on what they could prohibit in relation to reproductive health care. A significant check on those actions is now gone.

In the wake of Dobbs, societal debates about personhood have increased and expanded in terms of their practical implications. Before Dobbs, anti-abortion advocates admitted that Roe and subsequent cases limited the scope of their arguments, especially in relation to the legality …

Privacy Law's Incumbency Problem, Peter Ormerod

Faculty Publications

Policymakers and scholars concerned with the power of informational platforms are questioning how traditional doctrinal silos like privacy law and antitrust law interact in digital markets. Their interaction has taken on new urgency in recent years as states have enacted a flurry of consent-based privacy laws and as digital markets have become increasingly dominated by the same few firms

Contemporary debates about the interaction of competition and privacy tend to ask what role, if any, privacy should play in the antitrust analysis. Little has been written about how new privacy laws shape the competitive landscape. This Article argues that consent-based …

Variations Of Aquaculture Structures, Operations, And Maintenance With Increasing Ocean Energy, Kevin G. Heasman, Nicholas Scott, Tyler Sclodnick, Michael Chambers, Barry Costa-Pierce, Tobias Dewhurst, Wolf Isbert, Bela H. Buck

Faculty Publications

Aquaculture in exposed and/or distant ocean sites is an emerging industry and field of study that addresses the need to improve food security along with the challenges posed by expansion of urban and coastal stakeholders into nearshore and sheltered marine waters. This move necessitates innovative solutions for this industry to thrive in high-energy environments. Some innovative research has increased understanding of the physics, hydrodynamics, and structural requirements enabling the development of appropriate systems. The blue mussel (Mytilus edulis), the New Zealand green shell or green lipped mussel (Perna canaliculus), and the Pacific Oyster (Magallana gigas), are the primary targets for …

Variations Of Aquaculture Structures, Operations, And Maintenance With Increasing Ocean Energy, Kevin G. Heasman, Nicholas Scott, Tyler Sclodnick, Michael Chambers, Barry Costa-Pierce, Tobias Dewhurst, Wolf Isbert, Bela H. Buck

Faculty Publications

Aquaculture in exposed and/or distant ocean sites is an emerging industry and field of study that addresses the need to improve food security along with the challenges posed by expansion of urban and coastal stakeholders into nearshore and sheltered marine waters. This move necessitates innovative solutions for this industry to thrive in high-energy environments. Some innovative research has increased understanding of the physics, hydrodynamics, and structural requirements enabling the development of appropriate systems. The blue mussel (Mytilus edulis), the New Zealand green shell or green lipped mussel (Perna canaliculus), and the Pacific Oyster (Magallana gigas), are the primary targets for …

Oyster Grower Perspectives On Green Crab (Carcinus Maenas) Interactions: Using Applied Social Science To Inform Research And Engagement, Kelsey Meyer-Rust, Lindsey Williams, Krystin Ward, Bonnie Brown

Faculty Publications

Research that informs the New Hampshire Aquaculture industry, which has grown significantly over the past few years, and helps the overall associated watershed community to keep Great Bay Estuary healthy is vital. Specific study of green crabs, Carcinus maenas, and their potential effects on oysters, Crassostrea virginica, is important to gain a better understanding of the impact invasive species may have on these ecologically and economically important bivalves. The aim of this study was to document and understand New Hampshire (NH) oyster growers’ perspectives on the issue of green crab predation of oysters. Through a survey, this study documented a …

Oyster Grower Perspectives On Green Crab (Carcinus Maenas) Interactions: Using Applied Social Science To Inform Research And Engagement, Kelsey Meyer-Rust, Lindsey Williams, Krystin Ward, Bonnie Brown

Faculty Publications

Research that informs the New Hampshire Aquaculture industry, which has grown significantly over the past few years, and helps the overall associated watershed community to keep Great Bay Estuary healthy is vital. Specific study of green crabs, Carcinus maenas, and their potential effects on oysters, Crassostrea virginica, is important to gain a better understanding of the impact invasive species may have on these ecologically and economically important bivalves. The aim of this study was to document and understand New Hampshire (NH) oyster growers’ perspectives on the issue of green crab predation of oysters. Through a survey, this study documented a …

Joint Subarray Acoustic Tweezers Enable Controllable Cell Translation, Rotation, And Deformation, Liang Shen, Zhenhua Tian, Kaichun Yang, Joseph Rich, Jianping Xia, Neil Upreti, Jinxin Zhang, Chuyi Chen, Nanjing Hao, Zhichao Pei, Tony Jun Huang

Faculty Publications

Contactless microscale tweezers are highly effective tools for manipulating, patterning, and assembling bioparticles. However, current tweezers are limited in their ability to comprehensively manipulate bioparticles, providing only partial control over the six fundamental motions (three translational and three rotational motions). This study presents a joint subarray acoustic tweezers platform that leverages acoustic radiation force and viscous torque to control the six fundamental motions of single bioparticles. This breakthrough is significant as our manipulation mechanism allows for controlling the three translational and three rotational motions of single cells, as well as enabling complex manipulation that combines controlled translational and rotational motions. …