Digital Commons Network^™

Mtor Complex 1 Implicated In Aphid/Buchnera Host/Symbiont Integration, Edward B. James, Honglin Feng, Alex C.C. Wilson

Faculty Publications

Obligate nutritional endosymbioses are arguably the most intimate of all interspecific associations. While many insect nutritional endosymbioses are well studied, a full picture of how two disparate organisms, a bacterial endosymbiont and a eukaryotic host, are integrated is still lacking. The mTOR pathway is known to integrate nutritional conditions with cell growth and survival in eukaryotes. Characterization and localization of amino acid transporters in aphids suggest the mTOR pathway as a point of integration between an aphid host and its amino acid-provisioning endosymbiont Buchnera aphidicola. The mTOR pathway is unannotated in aphids and unstudied in any nutritional endosymbiosis. We annotated …

Production Of Live Young With Cryopreserved Sperm From The Endangered Livebearing Fish Redtail Splitfin (Xenotoca Eiseni, Rutter, 1896), Yue Liu, Harry Grier, Terrence R. Tiersch

Faculty Publications

Previous studies of sperm cryopreservation of livebearing fish have been limited to two genera within the family Poeciliidae. The goal of the present study was to investigate the feasibility to produce live young of livebearing goodeids (family Goodeidae) with cryopreserved sperm, using aquarium-trade populations of the endangered species Redtail Splitfin (Xenotoca eiseni, Rutter, 1896). Reproductive condition of females was evaluated by histological categorization of ovarian development. A total of 117 females were inseminated with cryopreserved sperm, 81 were inseminated with fresh sperm, 27 were mixed with males for natural breeding, and 30 were maintained without males or insemination. Histological images …

Isolation By Marine Barriers And Climate Explain Areas Of Endemism In An Island Rodent, Thomas C. Giarla, Sean P. Maher, Anang S. Achmadi, Meagan K. Moore, Mark T. Swanson, Kevin C. Rowe, Jacob A. Esselstyn

Faculty Publications

Aim: The Indonesian island of Sulawesi is home to thousands of endemic terrestrial animal species. Phylogeographical studies of some of these taxa revealed concordant distribution patterns (areas of endemism; AoEs), suggestive of a shared evolutionary or ecological mechanism driving divergence among various terrestrial taxa. Generally, AoEs have been attributed to Pleistocene marine incursions, geological fault zones and Sulawesi's history as an archipelago. We test, for the first time, the hypothesis that population divergences are associated with unsuitable climate spaces at the boundaries between these areas. Location: Sulawesi, Indonesia. Taxon: Maxomys musschenbroekii, a common murid rodent endemic to Sulawesi. Methods: We …

Ants Alter Molecular Characteristics Of Soil Organic Carbon Determined By Pyrolysis-Chromatography/Mass Spectrometry, Zhongsheng Zhang, Zhuo Wei, Jim Jian Wang, Ran Xiao, Yili Meng, Haitao Wu, Xianguo Lyu

Faculty Publications

Colonization by ecosystem engineers, such as ants, have important but occasionally unnoticed roles in regulating soil carbon cycles. However, little information is available pertaining to ant effects on changing molecular features of soil organic matter. In this study, we characterized total soil organic carbon (SOC), total nitrogen, water extractable organic carbon (WEOC) as well as molecular characteristics of humic acids (HAs) of ant mound soils (SoilAnt) and the surrounding soils (SoilCon) using pyrolysis-chromatography/mass (py-GC/MS). SOC, total nitrogen and WEOC contents of SoilAnt were significantly lower than those of SoilCon. SoilAnt samples had significant higher nitrogen containing compounds proportions in pyrolysis …

Investigation Of An Early Season River Flood Pulse: Carbon Cycling In A Subtropical Estuary, Benjamin J. Haywood, John R. White, Robert L. Cook

Faculty Publications

© 2018 Elsevier B.V. The January 2016 Bonnet Carré Spillway (BCS) opening resulted in a large-scale Mississippi River (MR) flood discharge that qualitatively and quantitatively impacted the dissolved organic matter (DOM) cycling in the Lake Pontchartrain Estuary (LPE) located in Louisiana, USA. This early season flood event was a result of the delay of snow formation caused by warmer than normal watershed temperatures. During the diversion period and the subsequent weeks, the LPE water temperature remained lower than pre-flood water temperatures, suppressing carbon cycling. Following that period, the water temperature increased, leading to an increase in the rate of abiotic …

Bgge: A New Package For Genomic-Enabled Prediction Incorporating Genotype × Environment Interaction Models, Italo Granato, Jaime Cuevas, Francisco Luna-Vázquez, Jose Crossa, Osval Montesinos-López, Juan Burgueño, Roberto Fritsche-Neto

Faculty Publications

One of the major issues in plant breeding is the occurrence of genotype × environment (GE) interaction. Several models have been created to understand this phenomenon and explore it. In the genomic era, several models were employed to improve selection by using markers and account for GE interaction simultaneously. Some of these models use special genetic covariance matrices. In addition, the scale of multi-environment trials is getting larger, and this increases the computational challenges. In this context, we propose an R package that, in general, allows building GE genomic covariance matrices and fitting linear mixed models, in particular, to a …

Seasonal Variations In The Siliciclastic Fluxes To The Western Philippine Sea And Their Impacts On Seawater ΕNd Values Inferred From 1 Year Of In Situ Observations Above Benham Rise, Zhaokai Xu, Tiegang Li, Christophe Colin, Peter D. Clift, Rongtao Sun, Zhaojie Yu, Shiming Wan, Dhongil Lim

Faculty Publications

©2018. The Authors. The Sr and Nd isotopic compositions of siliciclastic sediments and the Nd isotopic compositions of planktonic foraminifera are investigated in sediment trap samples collected in 2015 at water depths of 500 and 2,800 m to (1) track the seasonal changes in the sources and transport patterns of siliciclastic sediments to Benham Rise, (2) constrain the Nd isotopic compositions of the planktonic foraminifera throughout the water column, and (3) assess the influences of lithogenic inputs on the Nd isotopic compositions of the water masses. We demonstrate that volcanic matter and eolian dust are derived primarily from Luzon Island …

Teaching Population Health: Innovations In The Integration Of The Healthcare And Public Health Systems, Rosemary M. Caron, Anne M. Hewitt, Julie H. Carmalt, Edmond A. Hooker

Faculty Publications

Population health is a critical concept in healthcare delivery today. Many healthcare administrators are struggling to adapt their organization from fee-for-service to value delivery. Payers and patients expect healthcare leaders to understand how to deliver care under this new model. Health administration programs play a critical role in training future leaders of healthcare organizations to be adaptable and effective in this dynamic environment. The purpose of this research was to: (a) engage current educators of health administration students in a dialogue about the best practices of integrating the healthcare and public health systems; (b) identify the content and pedagogy for …

Pedagogy: How To Best Teach Population Health To Future Healthcare Leaders, Rosemary M. Caron, Edmond A. Hooker, Anne M. Hewitt, Julie H. Carmalt

Faculty Publications

Our healthcare system is moving from a fee-for-service reimbursement model to one that provides payment for improvements in three areas related to care: quality, coordination, and cost. Healthcare organizations must use a population health approach when delivering care under this new paradigm. Health administration programs play a critical role in training future leaders of healthcare organizations to be adaptable and effective in this dynamic environment. The purpose of this research was to: (1) engage health administration educators in a dialogue about population health and its relevance to healthcare administration education; (2) describe pedagogical methods appropriate for teaching population health skills …

Cause And Consequence Of Aβ: Lipid Interactions In Alzheimer Disease Pathogenesis, Vijay Rangachari, Dexter N. Dean, Pratip Rana, Ashwin Vaidya, Preetam Ghosh

Faculty Publications

Self-templating propagation of protein aggregate conformations is increasingly becoming a significant factor in many neurological diseases. In Alzheimer disease (AD), intrinsically disordered amyloid-β (Aβ) peptides undergo aggregation that is sensitive to environmental conditions. High-molecular weight aggregates of Aβ that form insoluble fibrils are deposited as senile plaques in AD brains. However, low-molecular weight aggregates called soluble oligomers are known to be the primary toxic agents responsible for neuronal dysfunction. The aggregation process is highly stochastic involving both homotypic (Aβ-Aβ) and heterotypic (Aβ with interacting partners) interactions. Two of the important members of interacting partners are membrane lipids and surfactants, to …

Challenges, Pitfalls And Surprises: Development And Validation Of A Monoclonal Antibody For Enzyme Immunoassay Of The Steroid 1Α-Hydroxycorticosterone In Elasmobranch Species, Catharine J. Wheaton, Natalie D. Mylniczenko, John M. Rimoldi, Rama S.V.S. Gadepalli, R. Hart, Bobbi R. O'Hara, Andrew N. Evans

Faculty Publications

Sharks and rays are popular species used in wildlife ecotourism and aquariums to educate the public on the behavior, ecology and conservation challenges of elasmobranchs. To understand long-term physiological health and welfare under varying social and husbandry conditions, we developed and validated an enzyme immunoassay (EIA) to measure stress/ionoregulatory hormones in managed and semi-free range southern rays (Hypanus americanus). Banked serum and interrenal samples from 27 female rays managed at Disney’s The Seas with Nemo and Friends^® and Castaway Cay were used to evaluate measurement of 1α-hydroxycorticosterone (1αOHB) relative to corticosterone (B). Although commercial EIAs are available …

Sex Differences In Rhesus Monkeys’ Digit Ratio (2d:4d Ratio) And Its Association With Maternal Social Dominance Rank, Alexander Baxter, Elizabeth K. Wood, Parker Jarman, Ashley N. Cameron, John P. Capitanio, James Dee Higley

Faculty Publications

Prenatal androgen exposure (PAE) plays a pivotal role in masculinizing the developing body and brain, and extreme exposure may contribute to autism, anxiety disorder and schizophrenia. One commonly used biomarker for PAE is the pointer-to-ring-finger digit length (2D:4D) ratio. Although this biomarker is widely used in human studies, relatively few studies have investigated 2D:4D ratio in nonhuman primates, particularly rhesus macaques (Macaca mulatta), one of the most commonly used animals in biomedical research. Thus far, data suggest that sexual dimorphism in 2D:4D ratio may be in the opposite direction in some monkey species, when compared to the pattern …

Influenza Virus Infectivity Is Retained In Aerosols And Droplets Independent Of Relative Humidity, Karen A. Kormuth, Kaisen Lin, Aaron J. Prussin, Eric P. Vejerano, Andrea J. Tiwari, Steve S. Cox, Micheal M. Myerburg, Seema S. Lakdawala, Linsey C. Marr

Faculty Publications

Pandemic and seasonal influenza viruses can be transmitted through aerosols and droplets, in which viruses must remain stable and infectious across a wide range of environmental conditions. Using humidity-controlled chambers, we studied the impact of relative humidity on the stability of 2009 pandemic influenza A(H1N1) virus in suspended aerosols and stationary droplets. Contrary to the prevailing paradigm that humidity modulates the stability of respiratory viruses in aerosols, we found that viruses supplemented with material from the apical surface of differentiated primary human airway epithelial cells remained equally infectious for 1 hour at all relative humidities tested. This sustained infectivity was …

Advancing Social Connection As A Public Health Priority In The United States, Julianne Holt-Lunstad, Theodore Robles, David A. Sbarra

Faculty Publications

A robust body of scientific evidence indicates that being embedded in high-quality close relationships and feeling socially connected to the people in your life is associated with decreased risk for all-cause mortality as well as a range of disease morbidities. Despite mounting evidence that the magnitude of these associations is comparable to many leading health determinants (that receive significant public health resources), government agencies, healthcare providers and associations, and public/private healthcare funders are slow to recognize human social relationships as either a health determinant or health risk marker in a manner that is comparable to other public health priorities. This …

Near-Infrared Photothermal Release Of Sirna From The Surface Of Colloidal Gold-Silver-Gold Core-Shell-Shell Nanoparticles Studied With Second-Harmonic Generation, Raju R. Kumal, Mohammad Abu-Laban, Prakash Hamal, Blake Kruger, Holden T. Smith, Daniel J. Hayes, Louis H. Haber

Faculty Publications

© 2018 American Chemical Society. Photothermal release of oligonucleotides from the surface of plasmonic nanoparticles represents a promising platform for spatiotemporal controlled drug delivery. Here we demonstrate the use of novel gold-silver-gold core-shell-shell (CSS) nanoparticles to study the photothermal cleaving and release of micro-RNA (miRNA) mimics or small interfering RNA (siRNA) under near-infrared (NIR) irradiation. The furan-maleimide-based Diels-Alder adduct cleaves thermally above 60 °C and is used to bind siRNA to the colloidal nanoparticle surface in water. We investigate the photothermal cleaving kinetics of siRNA under different NIR laser powers using surface-sensitive time-dependent second-harmonic generation (SHG) spectroscopy. The photothermal release …

Integrating Climate Forecasts With The Soil And Water Assessment Tool (Swat) For High-Resolution Hydrologic Simulations And Forecasts In The Southeastern U.S., Vinit Sehgal, Venkataramana Sridhar, Luke Juran, Jactone Arogo Ogejo

Faculty Publications

This study provides high-resolution modeling of daily water budget components at Hydrologic Unit Code (HUC)-12 resolution for 50 watersheds of the South Atlantic Gulf (SAG) region in the southeastern U.S. (SEUS) by implementing the Soil and Water Assessment Tool (SWAT) model in the form of a near real-time, semi-automated framework. A near real-time hydrologic simulation framework is implemented with a lead time of nine months (March-December 2017) by integrating the calibrated SWAT model with National Centers for Environmental Prediction coupled forecast system model version 2 (CFSv2) weather data to forecast daily water balance components. The modeling exercise is conducted as …

Curvilinear Association Between Language Disfluency And Fmr1 Cgg Repeat Size Across The Normal, Intermediate, And Premutation Range, Jessica Klusek, Ann Porter, Leonard Abbeduto, Tatyana Adayev, Flora Tassone, Marsha R. Mailick, Anne Glicksman, Bridgette L. Tonnsen, Jane E. Roberts

Faculty Publications

Historically, investigations of FMR1 have focused almost exclusively on the clinical effects of CGG expansion within the categories of the premutation (55–200 CGG repeats) and fragile X syndrome (>200 CGG repeats). However, emerging evidence suggests that CGG-dependent phenotypes may occur across allele sizes traditionally considered within the “normal” range. This study adopted an individual-differences approach to determine the association between language production ability and CGG repeat length across the full range of normal, intermediate, and premutation alleles. Participants included 61 adult women with CGG repeats within the premutation (n = 37), intermediate (i.e., 41–54 repeats; n = 2), …

Modeling The Influence Of Eutrophication And Redox Conditions On Mercury Cycling At The Sediment-Water Interface In The Berre Lagoon, Svetlana Pakhomova, Evgeniy Yakushev, Elizaveta Protsenko, Sylvain Rigaud, Daniel Cossa, Joel Knoery, Raoul Marie Couture, Olivier Radakovitch, Shamil Yakubov, Dominika Krzeminska, Alice Newton

Faculty Publications

© 2018 Pakhomova, Yakushev, Protsenko, Rigaud, Cossa, Knoery, Couture, Radakovitch, Yakubov, Krzeminska and Newton. This study presents a specifically designed Mercury module in a coupled benthic-pelagic reactive-transport model - Bottom RedOx Model (BROM) that allows to study mercury (Hg) biogeochemistry under different conditions. This module considers the transformation of elemental mercury (Hg(0)), divalent mercury (Hg(II)) and methylmercury (MeHg). The behavior of mercury species in the model is interconnected with changes of oxygen, hydrogen sulfide, iron oxides, organic matter, and biota. We simulated the transformation and transport of Hg species in the water column and upper sediment layer under five different …

Novel Measurements Of Fine-Scale Albedo: Using A Commercial Quadcopter To Measure Radiation Fluxes, Charlotte R. Levy, Elizabeth A. Burakowski, Andrew D. Richardson

Faculty Publications

Remote sensing of radiative indices must balance spatially and temporally coarse satellite measurements with finer-scale, but geographically limited, in-situ surface measurements. Instruments mounted upon an Unmanned Aerial Vehicle (UAV) can provide small-scale, mobile remote measurements that fill this resolution gap. Here we present and validate a novel method of obtaining albedo values using an unmodified quadcopter at a deciduous northern hardwood forest. We validate this method by comparing simultaneous albedo estimates by UAV and a fixed tower at the same site. We found that UAV provided stable albedo measurements across multiple flights, with results that were well within the range …

Roles Of Ros, Nrf2, And Autophagy In Cadmium-Carcinogenesis And Its Prevention By Sulforaphane, Yuting Wang, Ardhendu Kumar Mandal, Young O.K. Son, Poyil Pratheeshkumar, James T.F. Wise, Lei Wang, Zhuo Zhang, Xianglin Shi, Zhimin Chen

Faculty Publications

Environmental and occupational exposures to cadmium increase the risk of various cancers, including lung cancer. The carcinogenic mechanism of cadmium, including its prevention remains to be investigated. Using fluorescence and electron spin resonance spin trapping, the present study shows that in immortalized lung cells (BEAS-2BR cells), exposure cadmium generated reactive oxygen species (ROS). Through ROS generation, cadmium increased the protein level of TNF-α which activated NF-κB and its target protein COX-2, creating an inflammatory microenvironment. As measured by anchorage-independent colony formation assay, cadmium induced malignant cell transformation. Inhibition of ROS by antioxidants inhibited transformation, showing that ROS were important in …

A Distributed Semi-Supervised Platform For Dnase-Seq Data Analytics Using Deep Generative Convolutional Networks, Shayan Shams, Richard Platania, Joohyun Kim, Jian Zhang, Kisung Lee, Seungwon Yang, Seung Jong Park

Faculty Publications

A deep learning approach for analyzing DNase-seq datasets is presented, which has promising potentials for unraveling biological underpinnings on transcription regulation mechanisms. Further understanding of these mechanisms can lead to important advances in life sciences in general and drug, biomarker discovery, and cancer research in particular. Motivated by recent remarkable advances in the field of deep learning, we developed a platform, Deep Semi-Supervised DNase-seq Analytics (DSSDA). Primarily empowered by deep generative Convolutional Networks (ConvNets), the most notable aspect is the capability of semi-supervised learning, which is highly beneficial for common biological settings often plagued with a less sufficient number of …

A Distributed Semi-Supervised Platform For Dnase-Seq Data Analytics Using Deep Generative Convolutional Networks, Shayan Shams, Richard Platania, Joohyun Kim, Jian Zhang, Kisung Lee, Seungwon Yang, Seung Jong Park

Faculty Publications

A deep learning approach for analyzing DNase-seq datasets is presented, which has promising potentials for unraveling biological underpinnings on transcription regulation mechanisms. Further understanding of these mechanisms can lead to important advances in life sciences in general and drug, biomarker discovery, and cancer research in particular. Motivated by recent remarkable advances in the field of deep learning, we developed a platform, Deep Semi-Supervised DNase-seq Analytics (DSSDA). Primarily empowered by deep generative Convolutional Networks (ConvNets), the most notable aspect is the capability of semi-supervised learning, which is highly beneficial for common biological settings often plagued with a less sufficient number of …

The Tick Endosymbiont Candidatus Midichloria Mitochondrii And Selenoproteins Are Essential For The Growth Of Rickettsia Parkeri In The Gulf Coast Tick Vector, Khemraj Budachetri, Deepak Kumar, Gary Crispell, Christine Beck, Gregory Dasch, Shahid Karim

Faculty Publications

Background

Pathogen colonization inside tick tissues is a significant aspect of the overall competence of a vector. Amblyomma maculatum is a competent vector of the spotted fever group rickettsiae, Rickettsia parkeri. When R. parkeri colonizes its tick host, it has the opportunity to dynamically interact with not just its host but with the endosymbionts living within it, and this enables it to modulate the tick’s defenses by regulating tick gene expression. The microbiome in A. maculatum is dominated by two endosymbiont microbes: a Francisella-like endosymbiont (FLE) and CandidatusMidichloria mitochondrii (CMM). A range of selenium-containing proteins (selenoproteins) in A. …

Attitudes Toward Evidence-Based Practice, Occupational Stress And Work-Related Social Support Among Health Care Providers In China: A Sem Analysis, Shan Qiao Ph.D., Xiaoming Li Ph.D., Yuejiao Zhou, Zhiyong Shen, Bonita Stanton

Faculty Publications

Individuals’ attitudes toward evidence-based practices (EBP) are critical in adopting, implementing and maintaining the EBP in clinical settings. Multiple empirical studies have examined how work context may shape perceptions and attitudes towards EBP. The current study aims to further explore how both work and family contexts, as assessed by three psychosocial indicators (i.e., occupational stress, work-related social support from coworkers, and work-related social support from family), may affect attitudes toward EBP among health care providers in HIV clinics in China. We analyzed cross-sectional survey data from 357 health care providers recruited from 40 HIV clinics across 16 cities/counties in Guangxi …

Congenital Zika Virus Infection In Immunocompetent Mice Causes Postnatal Growth Impediment And Neurobehavioral Deficits, Fengwei Bai, Amber Paul, Dhiraj Acharya, Biswas Neupane, E.A. Thompson, Gabriel Gonzalez-Fernandez, Katherine Copeland, Melanae Garrett, Haibei Liu, Mariper Lopez, Matthew De Cruz, Alex S. Flynt, Jun Liao, Yan-Lin Guo, Federico Gonzalez-Fernandez, Parminder Vig

Faculty Publications

A small percentage of babies born to Zika virus (ZIKV)-infected mothers manifest severe defects at birth, including microcephaly. Among those who appeared healthy at birth, there are increasing reports of postnatal growth or developmental defects. However, the impact of congenital ZIKV infection in postnatal development is poorly understood. Here, we report that a mild congenital ZIKV-infection in pups born to immunocompetent pregnant mice did not display apparent defects at birth, but manifested postnatal growth impediments and neurobehavioral deficits, which include reduced locomotor and cognitive deficits that persisted into adulthood. We found that the brains of these pups were smaller, had …

Structure Based Modulation Of Electron Dynamics In Meso-(4-Pyridyl)-Bodipys: A Computational And Synthetic Approach, Daniel J. Lamaster, Nichole E.M. Kaufman, Adam S. Bruner, M. Graça H. Vicente

Faculty Publications

© 2018 American Chemical Society. The effects of structural modification on the electronic structure and electron dynamics of cationic meso-(4-pyridyl)-BODIPYs were investigated. A library of 2,6-difunctionalized meso-(4-pyridyl)-BODIPYs bearing various electron-withdrawing substituents was designed, and DFT calculations were used to model the redox properties, while TDDFT was used to determine the effects of functionalization on the excited states. Structural modification was able to restructure the low-lying molecular orbitals to effectively inhibit d-PeT. A new meso-(4-pyridyl)-BODIPY bearing 2,6-dichloro groups was synthesized and shown to exhibit enhanced charge recombination fluorescence. The fluorescence enhancement was determined to be the result of functionalization modulating the …

Soil Erodibility Differs According To Heritable Trait Variation And Nutrient-Induced Plasticity In The Salt Marsh Engineer Spartina Alterniflora, Brittany M. Bernik, John H. Pardue, Michael J. Blum

Faculty Publications

Use of landform engineers for habitat restoration has often resulted in unanticipated outcomes. It is possible that departures from expectation arise because applications do not adequately account for the influence of heritable and non-heritable phenotypic variation on ecosystem attributes. In this study, we performed a common garden greenhouse experiment to determine whether soil shear strength—a characteristic linked to erosion resistance—varies according to heritable and plastic trait expression in Spartina alterniflora grown under contrasting nutrient regimes. We detected heritable variation across a broad spectrum of functional traits, including nutrient uptake. We also found that S. alterniflora exhibited trait-specific differences in nutrient-induced …

Dietary Inflammatory Index And Biomarkers Of Lipoprotein Metabolism, Inflammation And Glucose Homeostasis In Adults, Catherine Phillips, Nitin Shivappa, James R. Hébert, Ivan Perry

Faculty Publications

Accumulating evidence identifies diet and inflammation as potential mechanisms contributing to cardiometabolic risk. However, inconsistent reports regarding dietary inflammatory potential, biomarkers of cardiometabolic health and metabolic syndrome (MetS) risk exist. Our objective was to examine the relationships between a food frequency questionnaire (FFQ)-derived dietary inflammatory index (DII®), biomarkers of lipoprotein metabolism, inflammation and glucose homeostasis and MetS risk in a cross-sectional sample of 1992 adults. Energy-adjusted DII (E-DII) scores derived from an FFQ were calculated. Lipoprotein particle size and subclass concentrations were measured using nuclear magnetic resonance (NMR) spectroscopy. Serum acute-phase reactants, adipocytokines, pro-inflammatory cytokines and white blood cell (WBC) …

Laboratory Growth Of Denitrifying Water Column Microbia Consortia From Deep-Sea Shipwrecks In The Northern Gulf Of Mexico, Dhanya Haridas, Justin C. Biffinger, Thomas J. Boyd, Preston A. Fulmer, Leila J. Hamdan, Lisa A. Fitzgerald

Faculty Publications

Background: Shipwrecks serve as a rich source for novel microbial populations that have largely remained undiscovered. Low temperatures, lack of sunlight, and the availability of substrates derived from the shipwreck’s hull and cargo may provide an environment in which microbes can develop unique metabolic adaptations.

Methods: To test our hypothesis that shipwrecks could influence the microbial population involved in denitrification when a consortium is grown in the laboratory, we collected samples proximate to two steel shipwrecks in the northern Gulf of Mexico. Then under laboratory conditions, we grew two independent denitrifying microbial consortia. Each consortium was grown by …

Effects Of Maternal Nutrition On The Expression Of Genomic Imprinted Genes In Ovine Fetuses, Jingyue (Ellie) Duan, Mingyuan Zhang, Kaleigh Flock, Sahar Al Seesi, Ion Mandoiu, Amanda Jones, Elizabeth Johnson, Sambhu Pillai, Maria Hoffman, Katelyn Mcfadden, Hesheng Jiang, Sarah Reed, Kristen Govoni, Steve Zinn, Zongliang Jiang, Xiuchun (Cindy) Tian

Faculty Publications

© 2018, © 2018 The Author (s). Published by Informa UK Limited, trading as Taylor & Francis Group. Genomic imprinting is an epigenetic phenomenon of differential allelic expression based on parental origin. To date, 263 imprinted genes have been identified among all investigated mammalian species. However, only 21 have been described in sheep, of which 11 are annotated in the current ovine genome. Here, we aim to i) use DNA/RNA high throughput sequencing to identify new monoallelically expressed and imprinted genes in day 135 ovine fetuses and ii) determine whether maternal diet (100%, 60%, or 140% of National Research Council …