Digital Commons Network^™

Genomic, Ecological, And Morphological Approaches To Investigating Species Limits: A Case Study In Modern Taxonomy From Tropical Eastern Pacific Surgeonfishes, Prosanta Chakrabarty

Faculty Publications

A wide variety of species are distinguished by slight color variations. However, molecular analyses have repeatedly demonstrated that coloration does not always correspond to distinct evolutionary histories between closely related groups, suggesting that this trait is labile and can be misleading for species identification. In the present study, we analyze the evolutionary history of sister species of Prionurus surgeonfishes in the Tropical Eastern Pacific (TEP), which are distinguished by the presence or absence of dark spots on their body. We examined the species limits in this system using comparative specimen-based approaches, a mitochondrial gene (COI), more than 800 nuclear loci …

Influence Of Sorghum Cultivar, Phenological Stage, And Fertilization On Development And Reproduction Of Melanaphis Sacchari (Hemiptera: Aphididae), Blake E. Wilson, Jeffrey Davis, Thomas Reagan, Luna Lama

Faculty Publications

The sugarcane aphid, Melanaphis sacchari (Zehntner) (Hemiptera: Aphididae), is an invasive pest of grain sorghum, Sorghum bicolor (L) Moench (Poaceae). Since its first outbreak in sorghum in 2013, severe infestations have spread throughout the southern USA, causing major economic losses. Whereas insecticide applications can mitigate some of the pest's impacts, a sustainable ecology-based management program is needed to reduce reliance on chemical control. Two greenhouse assays examined the influence of selected host plant characteristics on M. sacchari life table parameters. We studied the effects of silicon (rates equivalent to 0 and 3,360 kg silicon per ha) and nitrogen (rates equivalent …

Hemipteran Pests Of Sugarcane In North America, Blake E. Wilson

Faculty Publications

ing-sucking herbivores (Insecta: Hemiptera) represent one of the greatest threats to agricultural production worldwide. Hemipteran pests directly injure plants as well as vector disease-causing plant pathogens. Production of sugarcane (Saccharum spp.) in North America is impacted by a complex of Hemiptera including the sugarcane aphid, Melanaphis sacchari Zehntner (Aphididae); yellow sugarcane aphid, Sipha flava (Forbes) (Aphididae); West Indian canefly, Saccharosydne saccharivora (Westwood) (Delphacidae); sugarcane delphacid, Perkinsiella saccharicida Kirkaldy (Delphacidae); and sugarcane lace bug, Leptodictya tabida (Herric-Schaeffer) (Tingidae). None of these pests is consistently damaging to large amounts of sugarcane acreage, but regional outbreaks are common. The biology, ecology, and pest …

Temperature-Dependent Development And Host Range Of Crapemyrtle Bark Scale, Acanthococcus Lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), Zinan Wang, Yan Chen, Rodrigo Diaz

Faculty Publications

© 2019 Florida Entomological Society. All rights reserved. The crapemyrtle bark scale, Acanthococcus lagerstroemiae (Kuwana) (Hemiptera: Eriococcidae), is an invasive pest of crapemyrtles, Lagerstroemia spp. L. (Lythraceae) in the southeastern USA. Information about its temperature-dependent development and host range is limited. The objectives of this study were to evaluate the effects of temperature on the immature development of A. lagerstroemiae, and to determine plant species suitable for immature development and reproduction. Developmental time and survival of eggs were evaluated at 7 constant temperatures from 17.5 to 32 °C, and of nymphs at 20, 25, and 30 °C. Results suggested that …

Fifty Shades Of Brown: Macroevolution Of Plumage Brightness In The Furnariida, A Large Clade Of Drab Neotropical Passerines, Rafael S. Marcondes, Robb T. Brumfield

Faculty Publications

© 2019 The Author(s). Evolution © 2019 The Society for the Study of Evolution. Both natural and sexual selection are thought to affect the evolution of bird color. Most studies of the topic have focused on sexually dichromatic taxa and showy plumages, which are expected to be more influenced by social selection and usually result in increased conspicuousness. However, many bird clades display dull brown or gray plumages that vary greatly in brightness (lightness), but little in hue (shade). Here, we examine the macroevolution of brightness in one such clade, the Furnariida. We make comparisons across light environments, body parts, …

Age And Growth Of Gray Triggerfish (Balistes Capriscus) From A North-Central Gulf Of Mexico Artifical Reef Zone, Amanda E. Jefferson, Robert J. Allman, Ashley E. Pacicco, James S. Franks, Frank J. Hernandez, Mark A. Albins, Sean P. Powers, Robert L. Shipp, J. Marcus Drymon

Faculty Publications

The overexploitation of many traditionally targeted reef fishes such as red snapper (Lutjanus campechanus, Poey 1860), alongside the implementation of increasingly restrictive management measures on those species, has led to increased targeting of conventionally discarded Gulf of Mexico gray triggerfish (Balistes capriscus, Gmelin 1789) commercially and recreationally. The goal of this study was to assess age and growth of gray triggerfish from the Alabama Artificial Reef Zone in the north-central Gulf of Mexico. Gray triggerfish (n = 1135) were collected predominantly from artificial habitat during 1999 – 2017. Specimens were sexed macroscopically and ages were assigned …

Proximal Tubular Cell–Specific Ablation Of Carnitine Acetyltransferase Causes Tubular Disease And Secondary Glomerulosclerosis, Claudia Kruger, Trang Tiffany Nguyen, Chelsea Breaux, Alana Guillory, Margaret Mangelli, Kevin T. Fridianto, Jean Paul Kovalik, David H. Burk, Robert C. Noland, Randall Mynatt, Krisztian Stadler

Faculty Publications

© 2019 by the American Diabetes Association. Proximal tubular epithelial cells are highly energy demanding. Their energy need is covered mostly from mitochondrial fatty acid oxidation. Whether derailments in fatty acid metabolism and mitochondrial dysfunction are forerunners of tubular damage has been suggested but is not entirely clear. Here we modeled mitochondrial overload by creating mice lacking the enzyme carnitine acetyltransferase (CrAT) in the proximal tubules, thus limiting a primary mechanism to export carbons under conditions of substrate excess. Mice developed tubular disease and, interestingly, secondary glomerulosclerosis. This was accompanied by increased levels of apoptosis regulator and fibrosis markers, increased …

Amplification Dynamics Of Platy-1 Retrotransposons In The Cebidae Platyrrhine Lineage, Mark A. Batzer, Jessica M. Storer, Jackson R. Mierl, Sarah A. Brantley

Faculty Publications

Platy-1 elements are Platyrrhine-specific, short interspersed elements originally discovered in the Callithrix jacchus (common marmoset) genome. To date, only the marmoset genome has been analyzed for Platy-1 repeat content. Here, we report full-length Platy-1 insertions in other New World monkey (NWM) genomes (Saimiri boliviensis, squirrel monkey; Cebus imitator, capuchin monkey; and Aotus nancymaae, owl monkey) and analyze the amplification dynamics of lineage-specific Platy-1 insertions. A relatively small number of full-length and lineage-specific Platy-1 elements were found in the squirrel, capuchin, and owl monkey genomes compared with the marmoset genome. In addition, only a few older Platy-1 subfamilies were recovered in …

Differentiation Of Surface Water And Groundwater In A Karst System Using Anthropogenic Signatures, Joe Honings

Faculty Publications

Surface water-groundwater interaction within a karstic system enhances contaminant transport, making karst aquifers susceptible to anthropogenic practices. Contaminated waters related to agricultural and animal husbandry in northwestern Illinois (USA) prompted this investigation. Six streams and five springs were sampled for 16 parameters to assess anthropogenic influences. Statistical analyses revealed differences in 13 of 16 parameters between the stream and spring waters. Rock-water interaction was identified as the dominant mechanism defining the chemistry for both waters, which were classified as Ca-Mg HCO3. Elevated nitrate as nitrogen (NO3-N), chloride (Cl-), sodium, and potassium concentrations indicate that human activities have influenced the quality …

The Cure For Cultivating Fastidious Microbes, William Wischusen, Arundhati Bakshi, Austen T. Webber, Lorelei E. Patrick

Faculty Publications

Course-Based Undergraduate Research Experiences (CUREs) expand the scientific educational benefits of research to large groups of students in a course setting. As part of an ongoing effort to integrate CUREs into first-year biology labs, we developed a microbiology CURE (mCURE) that uses a modified dilution-to-extinction high throughput culturing protocol for isolating abundant yet fastidious aquatic bacterioplankton during one semester. Students learn common molecular biology techniques like nucleic acid extraction, PCR, and molecular characterization; read and evaluate scientific literature; and receive training in scientific communication through written and oral exercises that incorporate social media elements. In the first three semesters, the …

Virulence-Driven Trade-Offs In Disease Transmission: A Meta-Analysis*, Miguel A. Acevedo, Forrest P. Dillemuth, Andrew J. Flick, Matthew J. Faldyn, Bret D. Elderd

Faculty Publications

© 2019 The Author(s). Evolution © 2019 The Society for the Study of Evolution. The virulence–transmission trade-off hypothesis proposed more than 30 years ago is the cornerstone in the study of host–parasite co-evolution. This hypothesis rests on the premise that virulence is an unavoidable and increasing cost because the parasite uses host resources to replicate. This cost associated with replication ultimately results in a deceleration in transmission rate because increasing within-host replication increases host mortality. Empirical tests of predictions of the hypothesis have found mixed support, which cast doubt about its overall generalizability. To quantitatively address this issue, we conducted …

Demystifying Jadeite: An Underwater Maya Discovery At Ek Way Nal, Belize, Heather Mckillop, George Harlow, April Sievert, C. Wayne Smith, Michael C. Wiemann

Faculty Publications

Jadeite artefacts at Maya sites are normally associated with ritual and ceremonial locations, with high-quality jadeite reserved for elite objects. The discovery of a jadeite gouge with a wooden handle at a Classic Maya salt-working site submerged by sea-level rise - Ek Way Nal, Belize - is therefore unexpected and provides new information about the utilitarian use of this stone. The extremely high quality of this jadeite tool is particularly surprising, offering new insight into the Classic Maya exchange systems and the role of salt makers such as those based at Ek Way Nal.

An Effective Model For Engaging Faculty And Undergraduate Students In Neuroscience Outreach With Middle Schoolers, Peter J. Vollbrecht, Riley S. Frenette, Andrew J. Gall

Faculty Publications

Engaging undergraduate students in science outreach events is critical for improving future communication between scientists and community members. Outreach events are opportunities for faculty and undergraduates to utilize active learning strategies to engage non-scientists in scientific questions and principles. Through careful design of outreach events, undergraduate students can practice science communication skills while reaching populations of the public that remain underserved and underrepresented in scientific fields. Here we describe a classroom outreach event designed to give a broad overview of the field of neuroscience to middle school students of all backgrounds by delivering the content in school, during school hours. …

Sex-Specific Growth And Reproductive Dynamics Of Red Drum In The Northern Gulf Of Mexico, Corbin F. Bennetts, Robert T. Leaf, Nancy J. Brown-Peterson

Faculty Publications

The Red Drum Sciaenops ocellatus stock is heavily targeted in the Gulf of Mexico (GOM) by recreational fishers and supports a small commercial fishery in Mississippi. Despite their popularity, little recent work has been done to describe their life history. In this work, we describe sex‐specific growth and reproductive dynamics of Red Drum collected from the northern GOM from September 2016 through October 2017. We evaluated seven candidate growth models and found that the three‐parameter von Bertalanffy growth function (VBGF) was the best candidate length‐at‐age model. No significant difference in growth between sexes was observed with the three‐parameter VBGF, despite …

Importance Of Weak Trophic Interactions In The Structure Of The Food Web In La Paz Bay, Southern Gulf Of California: A Topological Approach, Andrés F. Navia, Sergio R. Maciel-Zapata, Adrián F. González-Acosta, Robert T. Leaf, Victor H. Cruz-Escalona

Faculty Publications

Here, we describe the trophic structure of the La Paz Bay, Baja California Sur, Mexico, and evaluate the role of weak trophic interactions in the ecosystem using a suite of topological indices. We used information derived from published and unpublished stomach contents analysis studies from the region to construct an unweighted and directional trophic network (comprising 250 nodes and 1528 associated interactions) to assess the impact of predators in three scenarios. Each scenario simulated the removal of weak interactions corresponding to removal of those nodes with trophic contributions less than 5%, 10%, and 15%. In each removal scenario, the nodes …

The Strength Of Migratory Connectivity For Birds En Route To Breeding Through The Gulf Of Mexico, Emily B. Cohen, Clark R. Rushing, Frank R. Moore, Michael T. Hallworth, Jeffrey A. Hostetler, Mariamar Gutierrez Ramirez, Peter P. Marra

Faculty Publications

The strength of migratory connectivity is a measure of the cohesion of populations among phases of the annual cycle, including breeding, migration, and wintering. Many Nearctic‐Neotropical species have strong migratory connectivity between breeding and wintering phases of the annual cycle. It is less clear if this strength persists during migration when multiple endogenous and exogenous factors may decrease the cohesion of populations among routes or through time along the same routes. We sampled three bird species, American redstart Setophaga ruticilla, ovenbird Seiurus aurocapilla, and wood thrush Hylocichla mustelina, during spring migration through the Gulf of Mexico region …

Intelligent Machine Learning: Tailor-Making Macromolecules, Yousef Mohammadi, Mohammad Reza Saeb, Alexander Penlidis, Esmaiel Jabbari, Florian J. Stadler, Philippe Zinck, Krzysztof Matyjaszewski

Faculty Publications

Nowadays, polymer reaction engineers seek robust and effective tools to synthesize complex macromolecules with well-defined and desirable microstructural and architectural characteristics. Over the past few decades, several promising approaches, such as controlled living (co)polymerization systems and chain-shuttling reactions have been proposed and widely applied to synthesize rather complex macromolecules with controlled monomer sequences. Despite the unique potential of the newly developed techniques, tailor-making the microstructure of macromolecules by suggesting the most appropriate polymerization recipe still remains a very challenging task. In the current work, two versatile and powerful tools capable of effectively addressing the aforementioned questions have been proposed and …

Mandatory Multilateralism, Evan J. Criddle, Evan Fox-Decent

Faculty Publications

This Article challenges the conventional wisdom that states are always free to choose whether to participate in multilateral regimes. International law often mandates multilateralism to ensure that state laws and practices are compatible with sovereign equality and joint stewardship. The Article maps mandatory multilateralism's domain, defines its requirements, and examines its application to three controversies: the South China Sea dispute, the United States' withdrawal from the 2015 Paris Agreement, and Bolivia's case against Chile in the International Court of Justice.

Uniting And Dividing Influences Of Religion In Marriage Among Highly Religious Couples, Heather H. Kelley, Loren D. Marks, David C. Dollahite

Faculty Publications

Previous research has suggested that religion can be both helpful and harmful. However, much oof the research on religion and families has employed relatively simple, distal measures of religion and has focused on predominantly only one side of the dualistic nature of religion. Drawing upon interviews with 198 religious couples (N = 396 individuals), the purpose of this study was to better understand how religion can have both a unifying and a dividing influence on marital relationships. Three overarching themes, accompanied by supporting primary qualitative data from participants, are presented. These themes include (a) how religious beliefs unite and …

Paternal Self-Efficacy: A Parenting Resilience Factor For Fathers With Depression, Mark Herrick Trahan, Kevin Shafer

Faculty Publications

Parental depression has a negative effect on child development including mental and physical health, language and development, and externalizing and internalizing behavior. This quantitative research study examined the relationship between paternal self-efficacy (PSE) and parenting behaviors often associated with paternal depression. Data from the Survey of Contemporary Fathers were used and responses from self-identified fathers (n = 1,156) on paternal involvement, warmth, harsh parenting practices, and parenting self-efficacy were analyzed to assess the association between depression and PSE on fathering behavior. Ordinary least squares regression analysis indicated that depression was associated with harsh parenting and parenting warmth, while parenting self-efficacy …

Autonomous Seawater PCo2 And Ph Time Series From 40 Surface Buoys And The Emergence Of Anthropogenic Trends, Adrienne J. Sutton, Richard A. Feely, Stacy Maenner-Jones, Sylvia Musielwicz, John Osborne, Colin Dietrich, Natalie Monacci, Jessica Cross, Randy Bott, Alex Kozyr, Andreas J. Andersson, Nicholas R. Bates, Wei-Jun Cai, Meghan F. Cronin, Eric H. De Carlo, Burke Hales, Stephan D. Howden, Charity M. Lee, Derek P. Manzello, Michael J. Mcphaden, Melissa Meléndez, John B. Mickett, Jan A. Newton, Scott E. Noakes, Jae Hoon Noh, Solveig R. Olafsdottir, Joseph E. Salisbury, Uwe Send, Thomas W. Trull, Douglas C. Vandemark, Robert A. Weller

Faculty Publications

Ship-based time series, some now approaching over 3 decades long, are critical climate records that have dramatically improved our ability to characterize natural and anthropogenic drivers of ocean carbon dioxide (CO₂) uptake and biogeochemical processes. Advancements in autonomous marine carbon sensors and technologies over the last 2 decades have led to the expansion of observations at fixed time series sites, thereby improving the capability of characterizing sub-seasonal variability in the ocean. Here , we present a data product of 40 individual autonomous moored surface ocean pCO₂ (partial pressure of CO₂) time series established between …

Removal Of Eriochrome Black T By Sulfate Radical Generated From Fe-Impregnated Biochar/Persulfate In Fenton-Like Reaction, Jong Hwan Park, Jim J. Wang, Negar Tafti, Ronald D. Delaune

Faculty Publications

A new heterogeneous Fenton catalyst, Fe-impregnated biochar catalyst (Fe-BC) was synthesized and evaluated in detail for its catalytic activity with sulfate radical under various conditions. The optimum conditions for the removal of Eriochrome Black T (EBT) by Fe-BC were determined as 0.27 g/L K2S2O8 (PS) and 0.5 g/L Fe-BC for 0.1 g/L EBT, and which resulted 85–88% removal efficiency within 2 h. The effective oxidation of EBT was observed over a pH range of 2–4 and temperature of 25–45 °C and maintained EBT removal efficiency of 77.6–92.7%. Under optimum conditions, the catalytic activity was faster in the order of PS …

Ultra-Strong Long-Chain Polyamide Elastomers With Programmable Supramolecular Interactions And Oriented Crystalline Microstructures, Lingzhi Song, Tianyu Zhu, Liang Yuan, Jiangjun Zhou, Yaqiong Zhang, Zhongkai Wang, Chuanbing Tang

Faculty Publications

Polyamides are one of the most important polymers. Long-chain aliphatic polyamides could bridge the gap between traditional polyamides and polyethylenes. Here we report an approach to preparing sustainable ultra-strong elastomers from biomass-derived long-chain polyamides by thiol-ene addition copolymerization with diamide diene monomers. The pendant polar hydroxyl and non-polar butyrate groups between amides allow controlled programming of supramolecular hydrogen bonding and facile tuning of crystallization of polymer chains. The presence of thioether groups on the main chain can further induce metal–ligand coordination (cuprous-thioether). Unidirectional step-cycle tensile deformation has been applied to these polyamides and significantly enhances tensile strength to over 210 …

Functional And Transcriptomic Characterization Of Carboplatin-Resistant A2780 Ovarian Cancer Cell Line, Tamara Viscarra, Kurt Buchegger, Ignacio Jofre, Ismael Riquelme, Louise Zanella, Michel Abanto, Alyssa C. Parker, Stephen R. Piccolo, Juan Carlos Roa, Carmen Ili, Priscilla Brebi

Faculty Publications

Background

Ovarian cancer is a significant cancer-related cause of death in women worldwide. The most used chemotherapeutic regimen is based on carboplatin (CBDCA). However, CBDCA resistance is the main obstacle to a better prognosis. An in vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize cellular and molecular changes of induced CBDCA-resistant ovarian cancer cell line A2780.

Methods

The cell selection strategy used in this study was a dose-per-pulse method using a concentration of 100 μM for 2 h. Once 20 cycles of exposure to …

The Role Of Temperature In Affecting Carry-Over Effects And Larval Competition In The Globally Invasive Mosquito Aedes Albopictus, Nnaemeka F. Ezeakacha, Donald Yee

Faculty Publications

Background

Ectotherms, like mosquitoes, have evolved specific responses to variation in environmental conditions like temperature, and these responses could confer a fitness benefit or cost when carried-over to different life stages. However, effects of temperature on animals with complex life-cycles often only focus on part of their life-cycle, or only consider how single aspects of life-history may carry over to new stages. Herein we investigated how temperature affects intraspecific larval competition and carry-over effects from larval to adult stages in the widespread invasive Asian tiger mosquito Aedes albopictus.

Methods

For larval competition, larvae were reared at three densities (10, …

Adaptation To Climate Change Through Genetic Accommodation And Assimilation Of Plastic Phenotypes, Morgan Kelly

Faculty Publications

© 2019 The Author(s) Published by the Royal Society. All rights reserved. Theory suggests that evolutionary changes in phenotypic plasticity could either hinder or facilitate evolutionary rescue in a changing climate. Nevertheless, the actual role of evolving plasticity in the responses of natural populations to climate change remains unresolved. Direct observations of evolutionary change in nature are rare, making it difficult to assess the relative contributions of changes in trait means versus changes in plasticity to climate change responses. To address this gap, this review explores several proxies that can be used to understand evolving plasticity in the context of …

Group-Based Physical Activity Trajectories In Children Transitioning From Elementary To High School, Russell R. Pate, Michaela A. Schenkelberg, Marsha Dowda, Kerry L. Mciver

Faculty Publications

Background

Physical activity has been observed repeatedly to decline as children transition into adolescence; however, few studies have explored the possibility that sub-groups of children experience unique patterns of change during this transition. The purpose of this longitudinal study was to examine the physical activity trajectories in clusters of youth transitioning from 5th to 11th grade.

Methods

Participants (n = 652) were recruited as 5th graders (ages 10–12 years) from elementary schools (n = 21) in two school districts. Demographic, anthropometric, and physical activity data were collected once per year when children were in 5th, 6th, 7th, 9th, and 11th …

High-Performing Pgm-Free Aemfc Cathodes From Carbon-Supported Cobalt Ferrite Nanoparticles, Xiong Peng, Varchaswal Kashyap, Benjamin Ng, Sreekumar Kurungot, Lianqin Wang, John R. Varcoe, Mustain E William

Faculty Publications

Efficient and durable non-precious metal electrocatalysts for the oxygen reduction reaction (ORR) are highly desirable for several electrochemical devices, including anion exchange membrane fuel cells (AEMFCs). Here, cobalt ferrite (CF) nanoparticles supported on Vulcan XC-72 carbon (CF-VC) were created through a facile, scalable solvothermal method. The nano-sized CF particles were spherical with a narrow particle size distribution. The CF-VC catalyst showed good ORR activity, possessing a half-wave potential of 0.71 V. Although the intrinsic activity of the CF-VC catalyst was not as high as some other platinum group metal (PGM)-free catalysts in the literature, where this catalyst really shined was …

Does Family Communication Matter? Exploring Knowledge Of Breast Cancer Genetics In Cancer Families, Deborah Himes, Sarah H. Davis, Jane Lassetter Phd, Rn, Neil E. Peterson, Margaret F. Clayton, Wendy C. Birmingham, Anita Y. Kinney

Faculty Publications

Purpose: Knowledge of breast cancer genetics is critical for those at increased hereditary risk who must make decisions about breast cancer screening options. This descriptive study explored theory-based relationships among cognitive and emotional variables related to knowledge of breast cancer genetics in cancer families. Methods: Participants included first-degree relatives of women with breast cancer who had received genetic counseling and testing. Study participants themselves did not have breast cancer and had not received genetic counseling or testing. Data were collected by telephone interviews and surveys. Variables analyzed included numeracy, health literacy, cancer-related distress, age, education, and the reported amount of …

Predicting Protein Residue-Residue Contacts Using Random Forests And Deep Networks, Joseph Luttrell Iv, Tong Liu, Chaoyang Zhang, Zheng Wang

Faculty Publications

Background: The ability to predict which pairs of amino acid residues in a protein are in contact with each other offers many advantages for various areas of research that focus on proteins. For example, contact prediction can be used to reduce the computational complexity of predicting the structure of proteins and even to help identify functionally important regions of proteins. These predictions are becoming especially important given the relatively low number of experimentally determined protein structures compared to the amount of available protein sequence data.

Results: Here we have developed and benchmarked a set of machine learning methods …