Digital Commons Network^™

Shinylearner: A Containerized Benchmarking Tool For Machine-Learning Classification Of Tabular Data, Stephen Piccolo, Terry J. Lee, Erica Suh, Kimball Hill

Faculty Publications

Background: Classification algorithms assign observations to groups based on patterns in data. The machine-learning community have developed myriad classification algorithms, which are used in diverse life science research domains. Algorithm choice can affect classification accuracy dramatically, so it is crucial that researchers optimize the choice of which algorithm(s) to apply in a given research domain on the basis of empirical evidence. In benchmark studies, multiple algorithms are applied to multiple datasets, and the researcher examines overall trends. In addition, the researcher may evaluate multiple hyperparameter combinations for each algorithm and use feature selection to reduce data dimensionality. Although software implementations …

Advances In The Neurophysiology Of Magnocellular Neuroendocrine Cells, Jeffrey G. Tasker, Masha Prager-Khoutorsky, Ryoichi Teruyama, José R. Lemos, William E. Amstrong

Faculty Publications

© 2020 British Society for Neuroendocrinology Hypothalamic magnocellular neuroendocrine cells have unique electrical properties and a remarkable capacity for morphological and synaptic plasticity. Their large somatic size, their relatively uniform and dense clustering in the supraoptic and paraventricular nuclei, and their large axon terminals in the neurohypophysis make them an attractive target for direct electrophysiological interrogation. Here, we provide a brief review of significant recent findings in the neuroplasticity and neurophysiological properties of these neurones that were presented at the symposium “Electrophysiology of Magnocellular Neurons” during the 13th World Congress on Neurohypophysial Hormones in Ein Gedi, Israel in April 2019. …

A One-Shot Learning Framework For Assessment Of Fibrillar Collagen From Second Harmonic Generation Images Of An Infarcted Myocardium, Qun Liu, Supratik Mukhopadhyay, Maria Ximena Bastidas Rodriguez, Xing Fu, Sushant Sahu, David Burk, Manas Gartia

Faculty Publications

© 2020 IEEE. Myocardial infarction (MI) is a scientific term that refers to heart attack. In this study, we infer highly relevant second harmonic generation (SHG) cues from collagen fibers exhibiting highly non-centrosymmetric assembly together with two-photon excited cellular autofluorescence in infarcted mouse heart to quantitatively probe fibrosis, especially targeted at an early stage after MI. We present a robust one-shot machine learning algorithm that enables determination of 2D assembly of collagen with high spatial resolution along with its structural arrangement in heart tissues post - MI with spectral specificity and sensitivity. Detection, evaluation, and precise quantification of fibrosis extent …

Systematics, Biogeography, And Diversification Of Scytalopus Tapaculos (Rhinocryptidae), An Enigmatic Radiation Of Neotropical Montane Birds, Carlos Daniel Cadena, Andrés M. Cuervo, Laura N. Céspedes, Gustavo A. Bravo, Niels Krabbe, Thomas S. Schulenberg, Graham E. Derryberry, Luis Fabio Silveira, Elizabeth P. Derryberry, Robb T. Brumfield, Jon Fjeldså

Faculty Publications

Copyright © American Ornithological Society 2020. All rights reserved. For permissions, e-mail: [email protected]. We studied the phylogeny, biogeography, and diversification of suboscine passerines in the genus Scytalopus (Rhinocryptidae), a widespread, species-rich, and taxonomically challenging group of Neotropical birds. We analyzed nuclear (exons, regions flanking ultraconserved elements) and mitochondrial (ND2) DNA sequence data for a taxonomically and geographically comprehensive sample of specimens collected from Costa Rica to Patagonia and Brazil. We found that Scytalopus is a monophyletic group sister to Eugralla and consists of 3 main clades roughly distributed in (1) the Southern Andes, (2) eastern Brazil, and (3) the Tropical …

Efficacy Of Entomopathogenic Nematodes Against The Sugarbeet Wireworm, Limonius Californicus (Mannerheim) (Coleoptera: Elateridae), Ramandeep Kaur Sandhi, David Shapiro-Ilan, Anamika Sharma, Gadi V.P. Reddy

Faculty Publications

Wireworms, the larval stage of click beetles (Coleoptera: Elateridae), are economically important soil-dwelling pests that attack many field crops worldwide. Wireworms have become a serious threat to spring wheat in the Northern Great Plains because of lack of effective control measures, creating a need for alternative control methods such as biological control with entomopathogenic nematodes (EPNs). A laboratory bioassay was used to test ten EPN strains and identify infective EPN strains against the sugarbeet wireworm, Limonius californicus (Mannerheim) (Coleoptera: Elateridae). Steinernema carpocapsae (Weiser) (All and Cxrd strains) and S. riobrave Cabanillas, Poinar, and Raulston (355 and 7–12 strains) killed 60–70% …

Assessing Field-Scale Variability Of Soil Hydraulic Conductivity At And Near Saturation, Xi Zhang, Ole Wendroth, Christopher Matocha, Junfeng Zhu, Javier Reyes

Faculty Publications

Saturated hydraulic conductivity (Ks) is a crucial hydraulic property for assessing soil water dynamics. Understanding the spatial variability of Ks in a field is important for site-specific resource management. However, direct measurement of hydraulic conductivity K as a function of soil water pressure head h [K(h)] is time consuming and laborious. Alternatively, pedotransfer functions (PTFs) have been developed to predict Ks indirectly based on more easily measurable soil properties. Although PTFs have been used for decades, their validity for estimating the field-scale spatial variability of Ks remains unclear. The objectives of this study were to characterize the spatial pattern of …

Nucleolar Stress In Drosophila Neuroblasts, A Model For Human Ribosomopathies, Sonu Shrestha Baral, Molly E. Lieux, Patrick J. Dimario

Faculty Publications

Different stem cells or progenitor cells display variable threshold requirements for functional ribosomes. This is particularly true for several human ribosomopathies in which select embryonic neural crest cells or adult bone marrow stem cells, but not others, show lethality due to failures in ribosome biogenesis or function (now known as nucleolar stress). To determine if various Drosophila neuroblasts display differential sensitivities to nucleolar stress, we used CRISPR-Cas9 to disrupt the Nopp140 gene that encodes two splice variant ribosome biogenesis factors (RBFs). Disruption of Nopp140 induced nucleolar stress that arrested larvae in the second instar stage. While the majority of larval …

A New Proposal For The M + A Methodology In Segregating Populations Of Cowpea, Renata Fernandes De Matos, Antônio Moreira Barroso Neto, Júlio César Dovale, Cândida Hermínia Campos De Magalhães Bertini, Roberto Fritsche-Neto

Faculty Publications

The evaluation of segregating populations in plant breeding programs is an onerous and time-consuming process. Early identification of populations with genetic potential can be done by m + a methodology. However, the possibility of a modification in the traditional methodology in order to make it more efficient, that is, faster and cheaper, was envisaged. Thus, the objective of this study was to compare the genetic gains obtained by both methodologies, the traditional one and the proposed modification. For this, ten segregating bean-cowpea populations were evaluated at two distinct levels of homozygous F3:4 and F3:5. Genetic values were predicted by two …

Environmental Control On The Distribution Of Metabolic Strategies Of Benthic Microbial Mats In Lake Fryxell, Antarctica, Megan L. Dillon, Ian Hawes, Anne D. Jungblut, Tyler J. Mackey, Jonathan A. Eisen, Peter T. Doran, Dawn Y. Sumner

Faculty Publications

© 2020 Dillon et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Ecological theories posit that heterogeneity in environmental conditions greatly affects community structure and function. However, the degree to which ecological theory developed using plant- and animal-dominated systems applies to microbiomes is unclear. Investigating the metabolic strategies found in microbiomes are particularly informative for testing the universality of ecological theories because microorganisms have far wider metabolic capacity than plants and animals. We …

Genomic Evidence For Formate Metabolism By As The Key To Unlocking Deep Carbon In Lost City Microbial Ecosystems, Julia M. Mcgonigle, Susan Q. Lang, William J. Brazelton

Faculty Publications

The Lost City hydrothermal field on the Mid-Atlantic Ridge supports dense microbial life on the lofty calcium carbonate chimney structures. The vent field is fueled by chemical reactions between the ultramafic rock under the chimneys and ambient seawater. These serpentinization reactions provide reducing power (as hydrogen gas) and organic compounds that can serve as microbial food; the most abundant of these are methane and formate. Previous studies have characterized the interior of the chimneys as a single-species biofilm inhabited by the Lost City , but they also indicated that this methanogen is unable to metabolize formate. The new metagenomic results …

Analysis Of Military Construction Cost Growth In Usaf Major Defense Acquisition Programs, Emily E. Angell, Edward D. White, Jonathan D. Ritschel, Alfred E. Thal Jr.

Faculty Publications

This study uses descriptive and inferential statistics to identify cost growth Analysis of Military Construction of military construction (MILCON) at the programmatic level, while bridging the gap between Selected Acquisition Report (SAR) estimates and actual project costs. Findings of this study aid the cost community with appropriate allocation of resources in developing these estimates. Overall, Major Defense Acquisition Programs (MDAP) appear to experience more negative growth (cost savings) in MILCON estimates on reviewed SARs— typically less than 0.2% of the total program cost. SAR estimates became more accurate from the first to last SAR in comparison to the total MILCON …

Single-Pulse, Kerr-Effect Mueller Matrix Lidar Polarimeter, Keyser, Christian K., Richard K. Martin, Helena Lopez-Aviles, Khanh Nguyen, Arielle M. Adams, Demetrios Christodoulides

Faculty Publications

We present a novel light detection and ranging (LiDAR) polarimeter that enables measurement of 12 of 16 sample Mueller matrix elements in a single, 10 ns pulse. The new polarization state generator (PSG) leverages Kerr phase modulation in a birefringent optical fiber, creating a probe pulse characterized by temporally varying polarization. Theoretical expressions for the Polarization State Generator (PSG) Stokes vector are derived for birefringent walk-off and no walk-off and incorporated into a time-dependent polarimeter signal model employing multiple polarization state analyzers (PSA). Polarimeter modeling compares the Kerr effect and electro-optic phase modulator–based PSG using a single Polarization State Analyzer …

Clinical And Cortical Decline In The Aphasic Variant Of Alzheimer’S Disease, Derin J. Cobia, Emily Joy Rogalski, Jaiashre Sridhar, Adam Martersteck, Benjamin Rader, Anupa K. Arora, Angela J. Fought, Eileen H. Bigio, Sandra Weintraub, Marek-Marsel Mesulam, Alfred Rademaker

Faculty Publications

Introduction—Primary progressive aphasia (PPA) displays variable progression trajectories that require further elucidation.

Methods—Longitudinal quantitation of atrophy and language over 12 months was completed for PPA patients with and without positive amyloid PET (PPAAβ+ and PPAAβ−), an imaging biomarker of underlying Alzheimer’s disease. Results—Over 12 months, both PPA groups showed significantly greater cortical atrophy rates in the left versus right hemisphere, with a more widespread pattern in PPAAβ+. The PPAAβ− group also showed greater decline in performance on most language tasks. There was no obligatory relationship between the logopenic PPA variant and amyloid status. Effect sizes from quantitative MRI data were …

Seasonal Patterns Of Surface Inorgamic Carbon System Variables In The Gulf Of Mexico Inferred From A Regional High-Resolution Ocean Biogeochemical Model, Fabian A. Gomez, Rik Wanninkhof, Leticia Barbero, Sang Ki Lee, Frank J. Hernandez

Faculty Publications

Uncertainties in carbon chemistry variability still remain large in the Gulf of Mexico (GoM), as data gaps limit our ability to infer basin-wide patterns. Here we configure and validate a regional high-resolution ocean biogeochemical model for the GoM to describe seasonal patterns in surface pressure of CO₂ (pCO₂), aragonite saturation state (ΩAr), and sea–air CO₂ flux. Model results indicate that seasonal changes in surface pCO₂ are strongly controlled by temperature across most of the GoM basin, except in the vicinity of the Mississippi–Atchafalaya river system delta, where runoff largely controls dissolved inorganic carbon (DIC) …

Community Dynamics Of Fish Assemblages On Mid-Shelf And Outer-Shelf Coral Reefs In The Northwestern Gulf Of Mexico, Lynne S. Wetmore, Michael A. Dance, Ronald L. Hill, Jay R. Rooker

Faculty Publications

Offshore coral reefs in the Gulf of Mexico (GOM) are unique model systems for examining the mechanisms structuring reef fish communities due to their substantial geographic isolation, and the presence of replicate experimental units of both low (mid-shelf) and high coral diversity (shelf-edge) reefs. Here, we examined the species assemblage structure of juvenile and adult fishes at two mid-shelf reefs (Sonnier and Stetson Bank) and two shelf-edge reefs (East and West Flower Garden Banks) in the northwestern GOM to evaluate the relative importance of habitat (i.e., coral diversity) vs. recruitment in structuring resident fish assemblages. Visual reef fish surveys (n …

Developing Outreach Events That Impact Underrepresented Students: Are We Doing It Right?, Andrew J. Gall, Peter J. Vollbrecht, Tristan Tobias

Faculty Publications

Many outreach programs share the common goals of serving underrepresented groups in STEM and improving public attitudes toward science. To meet these goals, scientists must find ways to both reach the appropriate audience and communicate the importance of science in meaningful and accessible ways. This requires careful consideration of the outreach method being used. Two common outreach methods include in‐school visits (scientist in the classroom) and science fairs or open houses. Here, we compare the effectiveness of these two outreach methods in meeting the goals of reaching underrepresented students and/or students with less initial interest in science. We have found …

Understanding Fluid Dynamics From Langevin And Fokker–Planck Equations, Andrei Medved, Riley Davis, Paula A. Vasquez

Faculty Publications

The Langevin equations (LE) and the Fokker–Planck (FP) equations are widely used to describe fluid behavior based on coarse-grained approximations of microstructure evolution. In this manuscript, we describe the relation between LE and FP as related to particle motion within a fluid. The manuscript introduces undergraduate students to two LEs, their corresponding FP equations, and their solutions and physical interpretation.

Functional Blockade Of E-Selectin In Tumor-Associated Vessels Enhances Anti-Tumor Effect Of Doxorubicin In Breast Cancer, Yoshihiro Morita, Macall Leslie, Hiroyasu Kameyama, Ganesh L. Lokesh, Norihisa Ichimura, Rachel Davis, Natalie Hills, Nafis Hasan, Roy Zhang, Yuji Kondo, David G. Gorenstein, David E. Volk, Inna Chervoneva, Hallgeir Rui, Takemi Tanaka

Faculty Publications

Chemotherapy is a mainstay of treatment for solid tumors. However, little is known about how therapy-induced immune cell infiltration may affect therapy response. We found substantial CD45 immune cell density adjacent to E-selectin expressing inflamed vessels in doxorubicin (DOX)-treated residual human breast tumors. While CD45 level was significantly elevated in DOX-treated wildtype mice, it remained unchanged in DOX-treated tumors from E-selectin null mice. Similarly, intravenous administration of anti-E-selectin aptamer (ESTA) resulted in a significant reduction in CD45 immune cell density in DOX-treated residual tumors, which coincided with a delay in tumor growth and lung metastasis in MMTV-pyMT mice. Additionally, both …

The Role Of Resilience In The Stem Identities Of Post-Secondary Students: A Qualitative Metasynthesis, Karen Benn Marshall, Sylvia M. James

Faculty Publications

The purpose of this qualitative metasynthesis is to explore the relationship between science, technology, engineering, and mathematics (STEM) identity and resilience in racially and ethnically diverse post-secondary students. This study identifies experiences that impact STEM identity development in diverse undergraduate and graduate students. The results of a constant targeted comparison (an analytical technique not often used in educational studies) between STEM identity and student resilience suggests that STEM identity contributes to resilience in diverse students. Findings suggest that common adverse experiences that may impact racially and ethnically diverse students can be overcome in the presence of a well-developed STEM identity. …

Inhibiting Iron Mobilization From Bacterioferritin In Pseudomonas Aeruginosa Impairs Biofilm Formation Irrespective Of Environmental Iron Availability, Anabel Soldano, Huili Yao, Josephine R. Chandler, Mario Rivera

Faculty Publications

Copyright © 2020 American Chemical Society. Although iron is essential for bacteria, the nutrient presents problems of toxicity and solubility. Bacteria circumvent these problems with the aid of iron storage proteins where Fe3+ is deposited and, when necessary, mobilized as Fe2+ for metabolic requirements. In Pseudomonas aeruginosa, Fe3+ is compartmentalized in bacterioferritin (BfrB), and its mobilization as Fe2+ requires specific binding of a ferredoxin (Bfd) to reduce the stored Fe3+. Blocking the BfrB-Bfd complex leads to irreversible iron accumulation in BfrB and cytosolic iron deprivation. Consequently, given the intracellular iron sufficiency requirement for biofilm development, we hypothesized that blocking the …

Comparative Analysis Of Rhizosphere Microbiomes Of Southern Highbush Blueberry (Vaccinium Corymbosum L.), Darrowâs Blueberry (V. Darrowii Camp), And Rabbiteye Blueberry (V. Virgatum Aiton), Jiangang Li, Olga V. Mavrodi, Jinfeng Hou, Chazden Blackmon, Ebrahiem M. Babiker, Dmitri V. Mavrodi

Faculty Publications

Plants are inhabited by millions of parasitic, commensal, and mutualistic microorganisms that coexist in complex ecological communities, and profoundly affect the plant’s productivity, health, and capacity to cope with environmental stress. Therefore, a better understanding of the rhizosphere microbiome may open a yet untapped avenue for the rational exploitation of beneficial plant–microbe interactions in modern agriculture. Blueberries encompass several wild and cultivated species of shrubs of the genus Vaccinium that are native to North America. They are grown commercially for the production of fruits, which are considered a health food due to the rich content of minerals, trace elements, and …

Listeria Monocytogenes Response To Anaerobic Environments, Brandy N. Roberts, Damayanti Chakravarty, J.C. Gardner Iii, Steven C. Ricke, Janet R. Donaldson

Faculty Publications

Listeria monocytogenes is a Gram-positive facultative anaerobic bacterium that is responsible for the disease, listeriosis. It is particularly lethal in pregnant women, the fetus, the elderly and the immunocompromised. The pathogen survives and replicates over a wide range of temperatures (4 to 42 °C), pH, salt and oxygen concentrations. Because it can withstand various environments, L. monocytogenes is a major concern in food processing industries, especially in dairy products and ready-to-eat fruits, vegetables and deli meats. The environment in which the pathogen is exposed can influence the expression of virulence genes. For instance, studies have shown that variations in oxygen …

Beyond Behavior: Linguistic Evidence Of Cultural Variation In Parental Ethnotheories Of Children’S Prosocial Helping, Andrew D. Coppens, Anna I. Corwin, Lucia Alcala

Faculty Publications

This study examined linguistic patterns in mothers’ reports about their toddlers’ involvement in everyday household work, as a way to understand the parental ethnotheories that may guide children’s prosocial helping and development. Mothers from two cultural groups – US Mexican-heritage families with backgrounds in indigenous American communities and middle-class European-American families – were interviewed regarding how their 2- to 3-year-old toddler gets involved in help with everyday household work. The study’s analytic focus was the linguistic form of mothers’ responses to interview questions asking about the child’s efforts to help with a variety of everyday household work tasks. Results showed …

Landscape Performance Of Buck Roses Under Minimal-Input Conditions In North-Central Texas, Derald A. Harp, Gaye Hammond, David C. Zlesak, Greg Church, Mark Chamblee, Steve George

Faculty Publications

Griffith Buck (Iowa State University) bred roses (Rosa sp.) to survive long, cold winters and hot, humid summers yet still retain their foliage without fungicides. Unfortunately, there is little known about the performance of Buck roses in the southern United States. Thirty-eight Buck rose cultivars were evaluated for flowering, disease resistance, drought tolerance, and overall landscape performance in alkaline soils with no fertilizer, no pesticides, and only limited irrigation. Flowering occurred on a bimodal basis, with the highest per plant mean bloom number (16.3 blooms) and bloom coverage (9.7%) in April, and a second flowering in the fall, with …

The Seasonal Evolution Of Albedo Across Glaciers And The Surrounding Landscape Of Taylor Valley, Antarctica, Anna Bergstrom, Michael N. Gooseff, Madeline Myers, Peter T. Doran, Julian M. Cross

Faculty Publications

© Author(s) 2020. The McMurdo Dry Valleys (MDVs) of Antarctica are a polar desert ecosystem consisting of alpine glaciers, ice-covered lakes, streams, and expanses of vegetation-free rocky soil. Because average summer temperatures are close to 0 °C, the MDV ecosystem in general, and glacier melt dynamics in particular, are both closely linked to the energy balance. A slight increase in incoming radiation or change in albedo can have large effects on the timing and volume of meltwater. However, the seasonal evolution or spatial variability of albedo in the valleys has yet to fully characterized. In this study, we aim to …

Hyaluronic Acid-Cellulose Composites As Patches For Minimizing Bacterial Infections, Kelsey M. Lopez, Sudhir Ravula, Rocío L. Pérez, Caitlan E. Ayala, Jack N. Losso, Marlene E. Janes, Isiah M. Warner

Faculty Publications

Copyright © 2020 American Chemical Society. A facile method was used to synthesize biocomposites containing differing ratios of hyaluronic acid (HA) and cellulose (CEL). Based on the properties of the individual polymers, the resultant composite materials may have potentially great wound care properties. In the method outlined here, 1-butyl-3-methylimidazolium chloride ([Bmim][Cl]), a simple ionic liquid, was used as the sole solvent without chemical modifiers to dissolve the biopolymers at ratios of 1:1 and 2:1 HA to CEL. This method was completely recyclable since the ionic liquid, [Bmim][Cl], can be recovered. Results from spectroscopic measurements [Fourier transform infrared (FT-IR) and X-ray …

Fully Automated Bone Age Assessment On Large-Scale Hand X-Ray Dataset, Xiaoyang Pan, Yizhe Zhao, Hao Chen, De Wei, Chen Zhao, Zhi Wei

Faculty Publications

Bone age assessment (BAA) is an essential topic in the clinical practice of evaluating the biological maturity of children. Because the manual method is time-consuming and prone to observer variability, it is attractive to develop computer-aided and automated methods for BAA. In this paper, we present a fully automatic BAA method. To eliminate noise in a raw X-ray image, we start with using U-Net to precisely segment hand mask image from a raw X-ray image. Even though U-Net can perform the segmentation with high precision, it needs a bigger annotated dataset. To alleviate the annotation burden, we propose to use …

A New Ectotherm 3d Tracking And Behavior Analytics System Using A Depth-Based Approach With Color Validation, With Preliminary Data On Kihansi Spray Toad (Nectophrynoides Asperginis) Activity, Philip Bal, Damian Lyons, Avishai Shuter

Faculty Publications

The Kihansi spray toad (Nectophrynoides asperginis), classified as Extinct in the Wild by the IUCN, is being bred at the Wildlife Conservation Society’s (WCS) Bronx Zoo as part of an effort to successfully reintroduce the species into the wild. Thousands of toads live at the Bronx Zoo presenting an opportunity to learn more about their behaviors for the first time, at scale. It is impractical to perform manual observations for long periods of time. This paper reports on the development of a RGB-D tracking and analytics approach that allows researchers to accurately and efficiently gather information about the toads’ behavior. …

Low-Phosphate Chromatin Dynamics Predict A Cell Wall Remodeling Network In Rice Shoots, Maryam Foroozani, Sara Zahraeifard, Dong Ha Oh, Guannan Wang, Maheshi Dassanayake, Aaron P. Smith

Faculty Publications

© 2020 American Society of Plant Biologists. All Rights Reserved. Phosphorus (P) is an essential plant macronutrient vital to fundamental metabolic processes. Plant-available P is low in most soils, making it a frequent limiter of growth. Declining P reserves for fertilizer production exacerbates this agricultural challenge. Plants modulate complex responses to fluctuating P levels via global transcriptional regulatory networks. Although chromatin structure plays a substantial role in controlling gene expression, the chromatin dynamics involved in regulating P homeostasis have not been determined. Here we define distinct chromatin states across the rice (Oryza sativa) genome by integrating multiple chromatin marks, including …

Genome-Wide Association Study To Identify Genetic Loci Associated With Gastrointestinal Nematode Resistance In Katahdin Sheep, G. M. Becker, K. M. Davenport, J. M. Burke, R. M. Lewis, J. E. Miller, J. L.M. Morgan, D. R. Notter, B. M. Murdoch

Faculty Publications

© 2020 The Authors. Animal Genetics published by John Wiley & Sons Ltd on behalf of Stichting International Foundation for Animal Genetics Resistance to gastrointestinal nematodes has previously been shown to be a moderately heritable trait in some breeds of sheep, but the mechanisms of resistance are not well understood. Selection for resistance currently relies upon faecal egg counts (FEC), blood packed cell volumes and FAMACHA visual indicator scores of anaemia. Identifying genomic markers associated with disease resistance would potentially improve the selection process and provide a more reliable means of classifying and understanding the biology behind resistant and susceptible …