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Effects Of Polyamine Biosynthesis Inhibitors On S-Adenosylmethionine Synthetase And S-Adenosylmethionine Decarboxylase Activities In Carrot Cell Cultures, Subhash C. Minocha, Rakesh Minocha, Atsushi Komamine Jan 1991

Effects Of Polyamine Biosynthesis Inhibitors On S-Adenosylmethionine Synthetase And S-Adenosylmethionine Decarboxylase Activities In Carrot Cell Cultures, Subhash C. Minocha, Rakesh Minocha, Atsushi Komamine

Faculty Publications

Changes in the activites of S-adcnosylmethionine (SAM) synthetase (methionine adenosyltransferase, EC 2.5.1.6.) and SAM decarboxylase (EC 4.1.1.50) were studied in carrot (Daucus carota) cell cultures in response to 2,4-dichlorophenoxyacetic acid (2,4-D) and several inhibitors of polyamine biosynthesis. Activity of SAM synthetase increased significantly during the first 5 days of culture under all conditions, maximum activity being observed in thc presence of 2,4-D. DL-α-Difluoromethylarginine (DFMA), I,L-α-difluoroinethylornithine (DFMO) and cyclohexylammonium sulfatc (CHAS) had little effect on the activity of this enzyme. The enzyme activity was lower than the controls in the presence of methylglyoxal bis(guany1hydrazone) (MGBG). The activity of SAM dccarboxylasc, which …

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Role Of A Carboxyl-Terminal Helix In The Assembly, Interchain Interactions, And Stability Of Aspartate Transcarbamoylase, Cynthia B. Peterson, H. K. Schachman Jan 1991

Role Of A Carboxyl-Terminal Helix In The Assembly, Interchain Interactions, And Stability Of Aspartate Transcarbamoylase, Cynthia B. Peterson, H. K. Schachman

Faculty Publications

The six individual catalytic polypeptide chains within the two catalytic trimers of Escherichia coli aspartate transcarbamoylase (ATCase; EC 2.1.3.2) are folded into two discrete structural domains interconnected in part by helix 12, which comprises residues 285-305 and is located near the carboxyl terminus of the chain. The essential role of this helix in folding of the chains and their assembly into ATCase was demonstrated by introducing a stop codon at the position corresponding to amino acid 284, 291, or 299. Cells containing these mutations are pyrimidine auxotrophs lacking ATCase-like protein in cell extracts. In contrast, stable active enzyme is formed …

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Accutane: Has Drug Regulation In The United States Reached Its Limits?, Joan H. Krause Jan 1991

Accutane: Has Drug Regulation In The United States Reached Its Limits?, Joan H. Krause

Faculty Publications

No abstract provided.

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Dna Evidence, Paul C. Giannelli Jan 1991

Dna Evidence, Paul C. Giannelli

Faculty Publications

No abstract provided.

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Competing In Global Product Innovation: Is Antitrust Immunity Necessary?, George J. Alexander Jan 1991

Competing In Global Product Innovation: Is Antitrust Immunity Necessary?, George J. Alexander

Faculty Publications

No abstract provided.

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Feminist Legal Scholarship, Patricia A. Cain Jan 1991

Feminist Legal Scholarship, Patricia A. Cain

Faculty Publications

No abstract provided.

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In Situ Degradation Of Polyhalogenated Aromatic Hydrocarbons By Electrochemically Generated Superoxide Ions, E. E. Kalu, Ralph E. White Jan 1991

In Situ Degradation Of Polyhalogenated Aromatic Hydrocarbons By Electrochemically Generated Superoxide Ions, E. E. Kalu, Ralph E. White

Faculty Publications

The reduction of dioxygen in aprotic media yields superoxide ions which react with polyhalogenated aromatic hydrocarbons by nucleophilic substitution. The degradation of hexachlorobenzene to bicarbonates and chlorides using in situ generated superoxide ions was carried out at room temperature in a flow cell system equipped with a gas fed, porous electrode. The effects of current, electrolyte flow, and aprotic media on the extent of degradation of hexachlorobenzene are presented.

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Book Review Of A General Theory Of Crime, Paul Marcus Jan 1991

Book Review Of A General Theory Of Crime, Paul Marcus

Faculty Publications

No abstract provided.

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Formation Of Methylmercaptan And Dimethylsulfide From Methoxylated Aromatic Compounds In Anoxic Marine And Fresh Water Sediments, Kai Finster, Gary M. King, Friedhelm Bak Dec 1990

Formation Of Methylmercaptan And Dimethylsulfide From Methoxylated Aromatic Compounds In Anoxic Marine And Fresh Water Sediments, Kai Finster, Gary M. King, Friedhelm Bak

Faculty Publications

Anaerobic formation of dimethylsulfide (DMS) and methylmercaptan (MSH) in anoxic sulfide-containing slurries from marine and fresh water sediments was stimulated by addition of syringate (4-hydroxy,3,5,-dimethoxybenzoate) and 3,4,5,-trimethoxybenzoate. The release of DMS and MSH occurred during the consumption of the aromatic monomers and ceased after their depletion. DMS was the dominant methylated sulfur compound in fresh water sediments, in contrast to marine sediments where MSH was predominant. No production of volatile organic sulfur compounds was observed in slurries containing gallate (3,4,5,-trihydroxybenzoate) or in autoclaved controled. About 50-65% of the methoxy carbon could be accounted for by peak accumulation of DMS and …

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Structure And Variability Of Recently Inserted Alu Family Members, Mark A. Batzer, Gail E. Kilroy, Pamela E. Richard, Tamim H. Shaikh, Trent D. Desselle, Carol L. Hoppens, Prescott L. Deininger Dec 1990

Structure And Variability Of Recently Inserted Alu Family Members, Mark A. Batzer, Gail E. Kilroy, Pamela E. Richard, Tamim H. Shaikh, Trent D. Desselle, Carol L. Hoppens, Prescott L. Deininger

Faculty Publications

The HS subfamily of Alu sequences is comprised of a group of nearly identical members. Individual subfamily members share 97.7% nucleotide identity with each other and 98.9% nucleotide identity with the HS consensus sequence. Individual subfamily members are on the average 2.8 million years old, and were probably derived from a single source 'master' gene sometime after the human/great ape divergence. The recent Alu family member insertions provide a better image of the structure of Alu retroposons before they have had the opportunity to change significantly. All of the HS subfamily members are flanked by perfect direct repeats as a …

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Genevan Scholars In Intellectual Europe: From The 17th Century To The Middle Of The 19th Century - French, Theodore S. Feldman Dec 1990

Genevan Scholars In Intellectual Europe: From The 17th Century To The Middle Of The 19th Century - French, Theodore S. Feldman

Faculty Publications

No abstract provided.

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Purification And Characterization Of A Dna Polymerase From The Cyanobacterium Anacystis Nidulans R2, Hsiu-Jin Lin, Gordon C. Cannon, Sabine Heinhorst Nov 1990

Purification And Characterization Of A Dna Polymerase From The Cyanobacterium Anacystis Nidulans R2, Hsiu-Jin Lin, Gordon C. Cannon, Sabine Heinhorst

Faculty Publications

A DNA polymerase has been highly purified from Anacystis nidulans R2. Electrophoretic analysis in sodium dodecyl sulfate-polyacrylamide gels revealed that the final fraction contains three bands of Mr 107,000, 93,000, and 51,000, respectively. Analysis of purified DNA polymerase activity in situ indicates that of the three polypeptides the Mr 107,000 species has the catalytic activities. The native molecular weight of the enzyme was estimated by glycerol gradient sedimentation to be 100,000. The enzyme has an absolute requirement for a divalent cation. Mg2+ can be replaced with Mn2+, but the DNA polymerase is less active. Potassium …

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Exercise Tolerance And Alcohol Intake: Blood Pressure Relation, G. Harley Hartung, Harold W. Kohl Iii, Steven N. Blair, Steven J. Lawrence, Ronald B. Harrist Nov 1990

Exercise Tolerance And Alcohol Intake: Blood Pressure Relation, G. Harley Hartung, Harold W. Kohl Iii, Steven N. Blair, Steven J. Lawrence, Ronald B. Harrist

Faculty Publications

The relations of systolic and diastolic blood pressures to alcohol intake and exercise tolerance levels in 15,612 men and 3,855 women were investigated. Alcohol intake was assessed by questionnaire and stratified into seven levels for men and six for women according to the ounces of ethanol consumed per week. Exercise tolerance was determined by maximal treadmill exercise testing and was categorized into six age-specific by sex-specific levels. Both systolic and diastolic blood pressure were significantly related to both alcohol intake and exercise tolerance levels in both men and women. These relations, which were positive for alcohol and negative for exercise …

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Products Liability: Principles Of Justice For The 21st Century, David G. Owen Oct 1990

Products Liability: Principles Of Justice For The 21st Century, David G. Owen

Faculty Publications

No abstract provided.

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Analysis Of Bacterial Genome Organization And Replication Using Pulsed-Field Gel Electrophoresis, A. Dingwall, L. Shapiro, Bert Ely Oct 1990

Analysis Of Bacterial Genome Organization And Replication Using Pulsed-Field Gel Electrophoresis, A. Dingwall, L. Shapiro, Bert Ely

Faculty Publications

The techniques described in this chapter provide a framework with which to begin an analysis of important features of bacterial genome organization and gene function. In this report we describe the preparation of chromosomal DNA, conditions for digestion with restriction endonucleases, and electrophoretic methods that result in the optimal resolution of large DNA restriction fragments. The sizes of the separated fragments can be summed to accurately determine genome size. The fragments can also be probed in hybridization experiments to produce low-resolution genomic restriction maps, and the precise locations of insertion or deletion mutations can be rapidly identified. In addition, PFGE …

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Relationships Between Wetland Fragmentation And Recent Hydrologic Changes In A Deltaic Coast, R. Eugene Turner, Yeleswarapu S. Rao Sep 1990

Relationships Between Wetland Fragmentation And Recent Hydrologic Changes In A Deltaic Coast, R. Eugene Turner, Yeleswarapu S. Rao

Faculty Publications

Patterns in coastal wetland loss in the northern Gulf of Mexico were examined using aerial imagery from 1955–56 and 1978. Five qualitative types of wetland changes are evident: (1) spoil bank-parallel pond formation, (2) pond formation with apparent random distribution for the smallest ponds, but very clumped distribution for larger ponds, (3) semi- or complete impoundment resulting in open water formation, (4) cutting off of stream channels upstream of where a spoil bank crosses a natural channel, and (5) erosion at the land-water interface. Only pondsinterval, and it is clear that wetland breakup, not erosion at the pond-lake edge, is …

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Theory Of Light Scattering By A Nonequilibrium Binary Mixture, J.C. Nieuwoudt, B.M. Law Aug 1990

Theory Of Light Scattering By A Nonequilibrium Binary Mixture, J.C. Nieuwoudt, B.M. Law

Faculty Publications

A binary mixture subject to a large temperature gradient is studied. Solutions of the evolution equations for the time-dependent fluctuation correlations are obtained using mode-coupling theory subject to the assumption that the mixture is not close to a critical point. From these results predictions for the light-scattering spectrum at the Rayleigh and Brillouin lines are made. The temperature gradient is shown to cause a large enhancement of the scattering amplitude at the Rayleigh line, whereas the effect at the Brillouin line is found to be small.

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Suppression Of Lymphocyte Proliferation By A > 30,000 Molecular Weight Factor In Horse Conceptus-Conditioned Medium, T. L. Roth, K. L. White, D. L. Thompson, B. E. Barry, J. S. Capehart, D. R. Colborn, M. H. Rabb Aug 1990

Suppression Of Lymphocyte Proliferation By A > 30,000 Molecular Weight Factor In Horse Conceptus-Conditioned Medium, T. L. Roth, K. L. White, D. L. Thompson, B. E. Barry, J. S. Capehart, D. R. Colborn, M. H. Rabb

Faculty Publications

In this experiment we have identified and partially characterized the immunosuppressive activity of preimplantation horse conceptus-conditioned medium (HCCM). Horse conceptuses were nonsurgically flushed from mares at Days 9-10 (n = 6), 15-16 (n = 3), and 25-26 (n = 3). After incubating the conceptuses for 24 h in RPMI-1640 supplemented with 15% fetal calf serum (FCS) and 1% penicillin/streptomycin, HCCM was obtained from cultures and tested for immunosuppressive activity in lymphocyte proliferation assays. Peripheral blood lymphocytes obtained from randomly selected mares were stimulated with mitogens (pokeweed mitogen [PWM], concanavalin A [Con A], and phytohemagglutinin [PHA]) in cultures supplemented with 0%, …

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Federal And State Water Quality Regulation And Law In Missouri, Peter N. Davis Apr 1990

Federal And State Water Quality Regulation And Law In Missouri, Peter N. Davis

Faculty Publications

This article discusses that law in two parts. The first part examines the federal and Missouri waste discharge regulatory system. The second part analyzes common law rights and remedies related to water pollution.

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Chloroplast And Mitochondrial: Dna Polymerases From Cultured Soybean Cells, Sabine Heinhorst, Gordon C. Cannon, Arthur Weissbach Apr 1990

Chloroplast And Mitochondrial: Dna Polymerases From Cultured Soybean Cells, Sabine Heinhorst, Gordon C. Cannon, Arthur Weissbach

Faculty Publications

DNA polymerases were purified from chloroplasts and mitochondria of cultured Glycine max cells. The chloroplast enzyme exists in two forms which are indistinguishable from each other biochemically. All three organellar enzymes have an estimated molecular weight of 85,000 to 90,000 and prefer poly(rA)dT12-18 over activated DNA as a template in vitro. Maximum activity of the chloroplast and mitochondrial DNA polymerases requires KCI and a reducing agent, and the enzymes are completely resistant to inhibitors of DNA polymerase a. Taken together, these properties classify the soybean organellar enzymes as DNA polymerases y. A unique feature that distinguishes the plant enzymes from …

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Environmental Water Rights: An Evolving Concept Of Public Property, Lynda L. Butler Apr 1990

Environmental Water Rights: An Evolving Concept Of Public Property, Lynda L. Butler

Faculty Publications

No abstract provided.

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Landscape Development And Coastal Wetland Losses In The Northern Gulf Of Mexico, R. Eugene Turner Feb 1990

Landscape Development And Coastal Wetland Losses In The Northern Gulf Of Mexico, R. Eugene Turner

Faculty Publications

The causes of the extensive (0.86%/yr; 288,414 ha/yr) and well documented dramatic and accelerating rate of coastal wetland loss in the northern Gulf of Mexico were investigated by an interdisciplinary university research team to discern the role of outer continental shelf development. The landscape changes and potential causal agents are emphasized herein. Natural driving factors include sea level rise and geological com- paction, which appear to remain constant this century, and sediment supply from the Mississippi River which has declined by 50%,since the 1950s. Man-made influences include hydrologic changes from river diversions, flood protection levees, an extensive canal and spoil …

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Inhomogeneous Broadening In Spectral Bands Of Carbonmonoxymyoglobin: The Connection Between Spectral And Functional Heterogeneity, Todd B. Sauke, P. Ormos, A. Ansari, D. Braunstein, B. R. Cowen, H. Fraunfelder, M. K. Hong, I. E. T. Iben, P. J. Steinbach, R. D. Young Jan 1990

Inhomogeneous Broadening In Spectral Bands Of Carbonmonoxymyoglobin: The Connection Between Spectral And Functional Heterogeneity, Todd B. Sauke, P. Ormos, A. Ansari, D. Braunstein, B. R. Cowen, H. Fraunfelder, M. K. Hong, I. E. T. Iben, P. J. Steinbach, R. D. Young

Faculty Publications

The rebinding kinetics of CO to myoglobin after flash photolysis is nonexponential in time below approximately 180 K; the kinetics is governed by a distribution of enthalpic barriers. This distribution results from inhomogeneities in the protein conformation, referred to as conformational substates. Hole-burning experiments on the Soret and IR CO-stretch bands test the assumption that an inhomogeneous distribution of conformational substates results in inhomogeneously broadened spectra. CO was slowly photolyzed at different wavelengths in the Soret band at 10 K. Both the Soret band and the CO-stretch band A1, centered at 1,945 cm-1, shift during photolysis, demonstrating that different wavelengths …

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Red-Cockaded Woodpeckers Vs Rat Snakes: The Effectiveness Of The Resin Barrier, D. Craig Rudolph, Howard Kyle, Richard N. Conner Jan 1990

Red-Cockaded Woodpeckers Vs Rat Snakes: The Effectiveness Of The Resin Barrier, D. Craig Rudolph, Howard Kyle, Richard N. Conner

Faculty Publications

Red-cockaded Woodpeckers (Picoides borealis) excavate resin wells in the immediate vicinity of roost and nest cavity entrances. Resin wells are worked regularly, resulting in a copious and persistent resin flow that coats the tree trunk, especially below cavity entrances. Red-cockaded Woodpeckers also scale loose bark from cavity trees and closely adjacent trees. These two behaviors result in smooth, sticky surfaces surrounding cavity entrances. Climbing experiments on cavity, scaled, and control trees using rat snakes (Elaphe obsoleta) demonstrate that these behaviors produce a resinous barrier that is highly effective in preventing predatory snakes from gaining access to active Red-cockaded Woodpecker cavities.

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Experimental Reintroduction Of Red-Cockaded Woodpeckers, D. Craig Rudolph, Howard Kyle, Richard N. Conner Jan 1990

Experimental Reintroduction Of Red-Cockaded Woodpeckers, D. Craig Rudolph, Howard Kyle, Richard N. Conner

Faculty Publications

The Red-cockaded Woodpecker (Picoides borealis) is an endangered species endemic to the pine forests of the southeastern United States (Jackson 1971). Deforestation and habitat alteration have severely affected Red-cockaded Woodpecker populations; current populations are isolated and most are declining (Jackson 1971, Lennartz et al. 1983, Conner and Rudolph 1989, Costa and Escano 1989). The species has been extirpated from significant areas of suitable or potentially suitable habitat.

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A New Chloroplast Protein Is Induced By Growth On Low Co2 In Chlamydomonas Reinhardtii, Catherine B. Mason, Livingston J. Manuel, James V. Moroney Jan 1990

A New Chloroplast Protein Is Induced By Growth On Low Co2 In Chlamydomonas Reinhardtii, Catherine B. Mason, Livingston J. Manuel, James V. Moroney

Faculty Publications

The biosynthesis of a 36 kilodalton polypeptide of Chlamydomonas reinhardtii was induced by photoautotrophic growth on low CO2. Fractionation studies using the cell-wall-deficient strain of C. reinhardtii, CC-400, showed that this polypeptide was different from the low CO2-induced periplasmic carbonic anhydrase. In addition, the 36 kilodalton polypeptide was found to be localized in intact chloroplasts isolated from low CO2-adapting cultures. This protein may, in part, account for the different inorganic carbon uptake characteristics observed in chloroplasts isolated from high and low CO2-grown C. reinhardtii cells.

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Effects Of Added Manganic And Ferric Oxides On Sulfate Reduction And Sulfide Oxidation In Intertidal Sediments, Gary M. King Jan 1990

Effects Of Added Manganic And Ferric Oxides On Sulfate Reduction And Sulfide Oxidation In Intertidal Sediments, Gary M. King

Faculty Publications

Freshly precipitated iron or manganese oxides were added to surface sediments from a salt marsh and from the intertidal region of Lowes Cove, Maine. In the presence of added manganese, sulfate was formed under anoxic conditions, suggesting a manganese dependent sulfide oxidation. Sulfate formation was not observed with iron additions. Sulfate reduction was substantially but not completely inhibited by either metal oxide, even though both were added at levels well in excess though both were added at levels well in excess of natural concentrations. Manganese-catalysed sulfide oxidation was further documented using a combination of radiolabel, metal oxide, and inhibitor additions, …

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Distribution And Rate Of Methane Oxidation In Sediments Of The Florida Everglades, G. M. King, P. Roslev, H. Skovgaard Jan 1990

Distribution And Rate Of Methane Oxidation In Sediments Of The Florida Everglades, G. M. King, P. Roslev, H. Skovgaard

Faculty Publications

Rates of methane emission from intact cores were measured during anoxic dark and oxic light and dark incubations. Rates of methane oxidation were calculated on the basis of oxic incubations by using the anoxic emissions as an estimate of the maximum potential flux. This technique indicated that methane oxidation consumed up to 91% of the maximum potential flux in peat sediments but that oxidation was negligible in marl sediments. Oxygen microprofiles determined for intact cores were comparable to profiles measured in situ. Thus, the laboratory incubations appeared to provide a reasonable approximation of in situ activities. This was further supported …

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Polyamine And Ethylene Biosynthesis In Relation To Somatic Embryogenesis In Carrot ( Daucus Carota L.) Cell Cultures, Subhash C. Minocha, Cheryl A. Robie, Akhtar Jamal Khan, Nancy S. Papa, Andrew I. Samuelsen, Rakesh Minocha Jan 1990

Polyamine And Ethylene Biosynthesis In Relation To Somatic Embryogenesis In Carrot ( Daucus Carota L.) Cell Cultures, Subhash C. Minocha, Cheryl A. Robie, Akhtar Jamal Khan, Nancy S. Papa, Andrew I. Samuelsen, Rakesh Minocha

Faculty Publications

Carrot cell cultures provide a model experimental system for the analysis of biochemical and molecular events associated with morphogenesis in plants (3, 4, 5, 14). Among the biochemical changes accompanying somatic embryogenesis in this tissue is an increased biosynthesis ofpolyamines (1, 2, 7, 10, 11, 13). A variety of inhibitors of polyamine biosynthetic enzymes have been used to analyze the role of polyamines in somatic embryogenesis. A summary of our work on the effects of DFMO, DFMA, MGBG and CHAP on somatic embryogenesis, cellular polyamine levels, and activities of ADC, ODC, AdoMet decarboxylase, and AdoMet-synthetase in carrot cell cultures is …

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The Burden Of Responsibility In The Libro De Buen Amor, Lynn Williams Jan 1990

The Burden Of Responsibility In The Libro De Buen Amor, Lynn Williams

Faculty Publications

In three of the early sections of the Libro de buen amor, Juan Ruiz explains to his public how, for various reasons, he is conditioned to love women. In stanzas 71-76 he uses the unimpeachable authority of Aristotle to argue that men and animals are biologically determined to seek 'juntamiento con fenbra plazentera' (st. 71d), and since he is a man like any other, it is natural, perhaps even inevitable, that from time to time he too should feel the attraction of the opposite sex. Paraphrasing St Paul (I Thessalonians 5.21), the Archpriest tells us rather cheekily that one must …

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