Digital Commons Network^™

Adherence To Accelerometer Protocols Among Women From Economically Disadvantaged Neighborhoods, Patricia A. Sharpe, Sarah Wilcox, Laura J. Rooney, Donna Strong, Rosie Hopkins-Campbell, Jean Butel, Barbara E. Ainsworth, Deborah Parra-Medina

Faculty Publications

Background: Objective measurement of physical activity with accelerometers is a challenging task in community-based intervention research. Challenges include distribution of and orientation to monitors, nonwear, incorrect placement, and loss of equipment. Data collection among participants from disadvantaged populations may be further hindered by factors such as transportation challenges, competing responsibilities, and cultural considerations. Methods: Research staff distributed accelerometers and provided an orientation that was tailored to the population group. General adherence strategies such as follow-up calls, daily diaries, verbal and written instructions, and incentives were accompanied by population-specific strategies such as assisting with transportation, reducing obstacles to wearing the accelerometer, …

Results Of The "Active By Choice Today" (Act) Randomized Trial For Increasing Physical Activity In Low-Income And Minority Adolescents, Dawn K. Wilson, M. Lee Van Horn, Heather Kitzman-Ulrich, Ruth P. Saunders, Russell R. Pate, Hannah G. Lawman, Brent Hutto, Sarah Griffin, Nicole Zarrett, Cheryl L. Addy, Leslie Mansard, Gary Mixon, Porschia V. Brown

Faculty Publications

Objective - This study reports the results of the "Active by Choice Today" (ACT) trial for increasing moderate-to-vigorous physical activity (MVPA) in low-income and minority adolescents.

Design - The ACT program was a randomized controlled school-based trial testing the efficacy of a motivational plus behavioral skills intervention on increasing MVPA in underserved adolescents. Twenty-four middle schools were matched on school size, percentage minorities, percentage free or reduced lunch, and urban or rural setting before randomization. A total of 1,563 6th grade students (mean age, 11.3 years, 73% African American, 71% free or reduced lunch, 55% female) participated in either a …

Altered Gene Expression And Spongiotrophoblast Differentiation In Placenta From A Mouse Model Of Diabetes In Pregnancy, J. M. Salbaum, C. Kruger, X. Zhang, N. Arbour Delahaye, G. Pavlinkova, D. H. Burk, C. Kappen

Faculty Publications

Aims/hypothesis: Pregnancies complicated by diabetes have a higher risk of adverse outcomes for mothers and children, including predisposition to disease later in life, e.g. metabolic syndrome and hypertension. We hypothesised that adverse outcomes from diabetic pregnancies may be linked to compromised placental function, and sought to identify cellular and molecular abnormalities in diabetic placenta. Methods: Using a mouse model of diabetic pregnancy, placental gene expression was assayed at mid-gestation and cellular composition analysed at various stages. Genome-wide expression profiling was validated by quantitative PCR and tissue localisation studies were performed to identify cellular correlates of altered gene expression in diabetic …

Maize-N: A Decision Tool For Nitrogen Management In Maize, T. D. Setiyono, H. Yang, D. T. Walters, A. Dobermann, R. B. Ferguson, D. F. Roberts, D. J. Lyon, D. E. Clay, K. G. Cassman

Faculty Publications

Nitrogen fertilizer efficiency has a large influence on profit, energy efficiency, N losses to the environment, and greenhouse gas emissions in maize (Zea mays L.) production. Our purpose was to develop a robust decision-support tool to help inform N fertilizer recommendations and to compare performance of this tool relative to existing recommendation approaches. Maize-N is a simulation model for estimating economically optimum N fertilizer rates (EONR) for maize. The model estimates the EONR based on uptake efficiency of the applied N, expected yield response, market prices of grain and N fertilizer, and mechanistic components of soil N mineralization. Uptake efficiency …

Serum Igf-1 Concentrations Change With Soy And Seaweed Supplements In Healthy Postmenopausal American Women, Jane Teas, Mohammad R. Irhimeh, Susan Druker, Thomas G. Hurley, James R. Hébert, Todd M. Savarese, Mindy S. Kurzer

Faculty Publications

Insulin-like growth factor 1 (IGF-1) is an anabolic hormone important for growth and development. However, high-circulating serum concentrations in adults are associated with increased risk of postmenopausal breast cancer. Nutritional status and specific foods influence serum IGF-1 concentrations. Breast cancer incidence is typically low in Asian countries where soy is commonly consumed. Paradoxically, soy supplement trials in American women have reported significant increases in IGF-1. Seaweed also is consumed regularly in Asian countries where breast cancer risk is low. We investigated the possibility that seaweed could modify soy-associated increases in IGF-1 in American women. Thirty healthy postmenopausal women (mean age …

Enabling Refugee And Idp Law And Policy: Implications Of The U.N. Disability Convention On The Rights Of Persons With Disabilities, Michael Ashley Stein, Janet E. Lord

Faculty Publications

No abstract provided.

The Gp130 Receptor Cytokine Family: Regulators Of Adipocyte Development And Function, Ursula A. White, Jacqueline M. Stephens

Faculty Publications

Gp130 cytokines are involved in the regulation of numerous biological processes, including hematopoiesis, immune response, inflammation, cardiovascular action, and neuronal survival. These cytokines share glycoprotein 130 as a common signal transducer in their receptor complex and typically activate STAT3. Most gp130 cytokines have paracrine or endocrine actions, and their levels can be measured in circulation in rodents and humans. In recent years, various laboratories have conducted studies to demonstrate that gp130 cytokines can modulate adipocyte development and function. Therefore, these studies suggest that some gp130 cytokines may be viable anti obesity therapeutics. In this review, we will summarize the reported …

Thermal Adaptation Of Net Ecosystem Exchange, W. Yuan, Y. Luo, S. Liang, G. Yu, S. Niu, P. Stoy, J. Chen, A. R. Desai, A. Lindroth, C. M. Gough, R. Ceulemans, A. Arain, C. Bernhofer, B. Cook, D. R. Cook, D. Dragoni, B. Gielen, I. A. Janssens, B. Longdoz, H. Liu, M. Lund, G. Matteucci, E. Moors, Ruth K. Varner

Faculty Publications

Thermal adaptation of gross primary production and ecosystem respiration has been well documented over broad thermal gradients. However, no study has examined their interaction as a function of temperature, i.e. the thermal responses of net ecosystem exchange of carbon (NEE). In this study, we constructed temperature response curves of NEE against temperature using 380 site-years of eddy covariance data at 72 forest, grassland and shrubland ecosystems located at latitudes ranging from ~29° N to 64° N. The response curves were used to define two critical temperatures: transition temperature (Tb) at which ecosystem transfer from carbon source to sink and optimal …

Evaluation Of Juvenile Hormone Analogues As Rodent Feed-Through Insecticides For Control Of Immature Phlebotomine Sandflies, T. M. Mascari, M. A. Mitchell, E. D. Rowton, L. D. Foil

Faculty Publications

The juvenile hormone analogues methoprene and pyriproxyfen were evaluated as rodent feed-through insecticides for control of immature stages of the sandfly Phlebotomus papatasi Scopoli (Diptera: Psychodidae). The development and survival of P. papatasi second-instar larvae fed faeces from Syrian hamsters, Mesocricetus auratus, that had been fed a diet containing methoprene (0, 9.788, 97.88 or 978.8 p.p.m.) or pyriproxyfen (0, 9.82, 98.2 or 982 p.p.m.) were evaluated. The faeces of methoprene-treated hamsters greatly reduced the percentage of larvae that pupated at all concentrations tested and prevented adult emergence at all but the lowest concentration (9.788 p.p.m.). Pyriproxyfen prevented both pupation and …

Developing Resistance To Aflatoxin In Maize And Cottonseed, Jeffrey W. Cary, Kanniah Rajasekaran, Robert L. Brown, Meng Luo, Zhi Yuan Chen, Deepak Bhatnagar

Faculty Publications

At this time, no ''magic bullet'' for solving the aflatoxin contamination problem in maize and cottonseed has been identified, so several strategies must be utilized simultaneously to ensure a healthy crop, free of aflatoxins. The most widely explored strategy for the control of aflatoxin contamination is the development of preharvest host resistance. This is because A. flavus infects and produces aflatoxins in susceptible crops prior to harvest. In maize production, the host resistance strategy has gained prominence because of advances in the identification of natural resistance traits. However, native resistance in maize to aflatoxin contamination is polygenic and complex and, …

Association Of Genetic Variation With Systolic And Diastolic Blood Pressure Among African Americans: The Candidate Gene Association Resource Study, Ervin R. Fox, J. Hunter Young, Yali Li, Albert W. Dreisbach, Brendan J. Keating, Solomon K. Musani, Kiang Liu, Alanna C. Morrison, Santhi Ganesh, Abdullah Kutlar, Vasan S. Ramachandran, Josef F. Polak, Richard R. Fabsitz, Daniel L. Dries, Deborah N. Farlow, Susan Redline, Adebowale Adeyemo, Joel N. Hirschorn, Yan V. Sun, Sharon B. Wyatt, Alan D. Penman, Walter Palmas, Jerome I. Rotter, Raymond R. Townsend, Ayo P. Doumatey, Bamidele O. Tayo, Thomas H. Mosley, Helen N. Lyon, Sun J. Kang, Charles N. Rotimi, Richard S. Cooper, Nora Franceschini, J. David Curb

Faculty Publications

The prevalence of hypertension in African Americans (AAs) is higher than in other US groups; yet, few have performed genome-wide association studies (GWASs) in AA. Among people of European descent, GWASs have identified genetic variants at 13 loci that are associated with blood pressure. It is unknown if these variants confer susceptibility in people of African ancestry. Here, we examined genome-wide and candidate gene associations with systolic blood pressure (SBP) and diastolic blood pressure (DBP) using the Candidate Gene Association Resource (CARe) consortium consisting of 8591 AAs. Genotypes included genome-wide singlenucleotide polymorphism (SNP) data utilizing the Affymetrix 6.0 array with …

Projecting Climate Change Effects On Forest Net Primary Productivity In Subtropical Louisiana, Usa, Fugui Wang, Y. Jun Xu, Thomas J. Dean

Faculty Publications

This study projected responses of forest net primary productivity (NPP) to three climate change scenarios at a resolution of 5 km × 5 km across the state of Louisiana, USA. In addition, we assessed uncertainties associated with the NPP projection at the grid and state levels. Climate data of the scenarios were derived from Community Climate System Model outputs. Changes in annual NPP between 2000 and 2050 were projected with the forest ecosystem model PnET-II. Results showed that forest productivity would increase under climate change scenarios A1B and A2, but with scenario B1, it would peak during 2011-2020 and then …

Hub City Steps: Methods And Early Findings From A Community-Based Participatory Research Trial To Reduce Blood Pressure Among African Americans, Jamie M. Zoellner, Carol L. Connell, Michael B. Madson, Bo Wang, Vickie Blakely Reed, Elaine F. Molaison, M. Kathleen Yadrick

Faculty Publications

Background: Community-based participatory research (CBPR) has been recognized as an important approach to develop and execute health interventions among marginalized populations, and a key strategy to translate research into practice to help reduce health disparities. Despite growing interest in the CBPR approach, CBPR initiatives rarely use experimental or other rigorous research designs to evaluate health outcomes. This behavioral study describes the conceptual frameworks, methods, and early findings related to the reach, adoption, implementation, and effectiveness on primary blood pressure outcomes.

Methods: The CBPR, social support, and motivational interviewing frameworks are applied to test treatment effects of a two-phased CBPR walking …

The Effects On Population Health Status Of Using Dedicated Property Taxes To Fund Local Public Health Agencies, Peggy A. Honoré, Xueyuan Wang

Faculty Publications

Background: In the United States, a dedicated property tax describes the legal authority given to a local jurisdiction to levy and collect a tax for a specific purpose. We investigated for an association of locally dedicated property taxes to fund local public health agencies and improved health status in the eight states designated as the Mississippi Delta Region.

Methods: We analyzed the difference in health outcomes of counties with and without a dedicated public health tax after adjusting for a set of control variables using regression models for county level data from 720 counties of the Mississippi Delta …

Cardiometabolic Results From An Armband-Based Weight Loss Trial, John C. Sieverdes, Xuemei Sui, Gregory A. Hand, Vaughn W. Barry, Sarah Wilcox, Rebecca A. Meriwether, James W. Hardin, Amanda C. Mcclain, Steven N. Blair

Faculty Publications

Purpose: This report examines the blood chemistry and blood pressure (BP) results from the Lifestyle Education for Activity and Nutrition (LEAN) study, a randomized weight loss trial. A primary purpose of the study was to evaluate the effects of real-time self-monitoring of energy balance (using the SenseWear^TM Armband, BodyMedia, Inc Pittsburgh, PA) on these health factors.

Methods: 164 sedentary overweight or obese adults (46.8 ± 10.8 years; BMI 33.3 ± 5.2 kg/m²; 80% women) took part in the 9-month study. Participants were randomized into 4 conditions: a standard care condition with an evidence-based weight loss manual (n …

Assessing Physical Activity And Its Relationship To Cardiovascular Risk Factors: Nhanes 2003-2006, Amy Luke, Lara R. Dugas, Ramon A. Durazo-Arvizu, Guichan Cao, Richard S. Cooper

Faculty Publications

Background: Levels of physical activity (PA) in the general population are difficult to characterize. Historically measurement has been based on self-report, which can be subject to bias. PA monitor use has created opportunities to improve surveillance and analytic research on activity and health. The aims of the current study were to investigate the associations between objectively measured PA and cardiovascular disease risk factors and obesity. Methods. Data on PA from accelerometers, demographics, blood pressure, plasma glucose and lipids, self-reported hypertension and diabetes were obtained for adults, ages 20-65, in the NHANES surveys, 2003-2006. Outcomes were assessed as levels of moderate …

The Public Choice Problem In Corporate Law: Corporate Social Responsibility After Citizens United, David Yosifon

Faculty Publications

The Supreme Court held in Citizens United v. Federal Elections Commission (2010) that the First Amendment forbids Congress from restricting the political speech of corporations. While corporate theory did little to inform the Court’s thinking in Citizens United, this Article argues that the holding in Citizens United requires us to rethink corporate theory. The shareholder primacy norm in American corporate governance relies on the assumption that corporations can be restrained from influencing external governmental operations. We can enjoy the efficiencies generated by shareholder primacy in corporate governance, mainstream corporate theorists have long argued, because we can rely on external regulation …

Simulated Reductions In Consumption Of Sugar-Sweetened Beverages Improves Diet Quality In Lower Mississippi Delta Adults, Jessica L. Thomson, Lisa M. Tussing-Humphreys, Stephen J. Onufrak, Carol Connell, Jamie M. Zoellner, Margaret L. Bogle, Kathleen Yadrick

Faculty Publications

Background

Although the effects of replacing sugar-sweetened beverages (SSBs) with water on energy intake and body weight have been reported, little is known about how these replacements affect diet quality.

Objective

To simulate the effects of replacing SSBs with tap water on diet quality and total energy intake of Lower Mississippi Delta (LMD) adults.

Design

Retrospective analysis of cross-sectional dietary intake data using a representative sample of LMD adults (n=1,689). Diet quality was measured using the Healthy Eating Index-2005 (HEI-2005) scores that were computed using the population ratio method. The effects of substituting SSBs with water on diet …

Electronic Feedback In A Diet- And Physical Activity-Based Intervention For Weight Loss: A Randomized Controlled Trial, Sara L. Shuger, Vaughn W. Barry, Xuemei Sui, Amanda C. Mcclain, Gregory A. Hand, Sarah Wilcox, Rebecca A. Meriwether, James W. Hardin, Steven N. Blair

Faculty Publications

Background: The SenseWear^TM Armband (SWA) (BodyMedia, Inc. Pittsburgh, PA) is a physical activity and lifestyle monitor that objectively and accurately measures free-living energy balance and sleep and includes software for self-monitoring of daily energy expenditure and energy intake. The real-time feedback of the SWA can improve individual self-monitoring and, therefore, enhance weight loss outcomes.

Methods: We recruited 197 sedentary overweight or obese adults (age, 46.8 ± 10.8 y; body mass index (BMI), 33.3 ± 5.2 kg/m²; 81% women, 32% African-American) from the greater Columbia, South Carolina area. Participants were randomized into 1 of 4 groups, a self-directed …

Autoregulation Of An Rna Polymerase Ii Promoter By The Rna Polymerase Iii Transcription Factor Iii C (TfIiiC) Complex, Richard A. Kleinschmidt, Kimberly E. Leblanc, David Donze

Faculty Publications

Extra TFIIIC (ETC) sites are chromosomal locations bound in vivo by the RNA polymerase III (Pol III) transcription factor III C (TF IIIC) complex, but are not necessarily associated with Pol III transcription. Although the location of ETC sequences are conserved in budding yeast, and similar sites are found in other organisms, their functions are largely unstudied. One such site, ETC6 in Saccharomyces cerevisiae, lies upstream of TFC6, a gene encoding a subunit of the TFIIIC complex itself. Promoter analysis shows that the ETC6 B-box sequence is involved in autoregulation of the TFC6 promoter. Mutation of ETC6 increases TFC6 mRNA …

Point-Of-Purchase Nutrition Information Influences Food-Purchasing Behaviors Of College Students: A Pilot Study, Marjorie Freedman, Rachel Conners

Faculty Publications

The goal of point-of-purchase (POP) nutrition information is to help consumers make informed, healthful choices. Despite limited evaluation, these population-based approaches are being advocated to replace traditional, more expensive, individual behavior-change strategies. Few studies have examined the effect of POP information on buying patterns of college students, a group with high obesity rates and poor eating habits. This quasi-experimental pilot project sought to determine whether the “Eat Smart” POP program affected food-purchasing habits of multiethnic college students shopping at an on-campus convenience store. Baseline sales data of foods in the cereal, soup, cracker, and bread categories were collected for 6 …

Forage Conservation Efficiency And Lactation Response To Bahiagrass Conserved As Barn-Stored Hay, Outdoor-Stored Hay, Or Baleage, M. E. Mccormick, K. J. Han, V. R. Moreira, D. C. Blouin, S. Forbes

Faculty Publications

A study was conducted to determine the effect of conserving bahiagrass as outdoor-stored hay (OSH), barn-stored hay (BSH), or baleage (BAL) on storage losses and lactation performance by Holstein cows. For baleage production, bahiagrass forage was cut and allowed to dry for 20. h in windrows, baled (1.2×1.4. m), and individually wrapped with white stretch film. Forage cut on the same day was dried to hay stage, baled (1.2×1.6. m round bales), and stored either outdoors (OSH) or in an enclosed hay barn (BSH). Bales were core sampled and weighed at harvest and again following a 6-mo storage period. At …

Bmi Trajectories During The Transition To Older Adulthood: Persistent, Widening, Or Diminishing Disparities By Ethnicity And Education?, Katrina M. Walsemann, Jennifer A. Ailshire

Faculty Publications

Previous research has produced inconsistent results on whether education and ethnic disparities in BMI persist, widen, or diminish over time. We investigate how education and ethnicity, independently and conditionally, influence BMI trajectories during the transition to older adulthood. Employing random coefficient modeling, we analyzed 8 biennial waves of data (1992-2006) from the Health and Retirement Study, a nationally representative longitudinal study of individuals born between 1931 and 1941. After adjusting for health behaviors and health status, education and ethnic disparities in BMI persisted for most groups, but narrowed between high-educated white men and both low-educated Hispanic men and high-educated black …

Wood Consumption By Formosan Subterranean Termites (Isoptera: Rhinotermitidae) As Affected By Wood Moisture Content And Temperature, Bal K. Gautam, Gregg Henderson

Faculty Publications

Understanding foraging behaviors and food preferences of termite colonies is critical to optimizing control strategies. The relationship between the moisture content (MC) of wood blocks and the feeding preference of Coptotermes formosanus Shiraki (Isoptera: Rhinotermitidae) at three ambient temperatures (19, 28, and 35°C) was investigated with multiple-choice as well as no-choice feeding bioassays. In multiple-choice tests, when four wood blocks with initial MC as dry (0-3%), low MC (22-24%), medium MC (70-90%), and high MC (125-150%) were exposed to workers and soldiers, the highest consumption was found in the high MC wood blocks at all three temperatures, although high MC …

Template To Improve Glycemic Control Without Reducing Adiposity Or Dietary Fat, R. Krishnapuram, E. J. Dhurandhar, O. Dubuisson, H. Kirk-Ballard, S. Bajpeyi, N. Butte, M. S. Sothern, E. Larsen-Meyer, S. Chalew, B. Bennett, A. K. Gupta, F. L. Greenway, W. Johnson, M. Brashear, G. Reinhart, T. Rankinen, C. Bouchard, W. T. Cefalu, J. Ye, R. Javier, A. Zuberi, N. V. Dhurandhar

Faculty Publications

Drugs that improve chronic hyperglycemia independently of insulin signaling or reduction of adiposity or dietary fat intake may be highly desirable. Ad36, a human adenovirus, promotes glucose uptake in vitro independently of adiposity or proximal insulin signaling. We tested the ability of Ad36 to improve glycemic control in vivo and determined if the natural Ad36 infection in humans is associated with better glycemic control. C57BL/6J mice fed a chow diet or made diabetic with a high-fat (HF) diet were mock infected or infected with Ad36 or adenovirus Ad2 as a control for infection. Postinfection (pi), systemic glycemic control, hepatic lipid …

Physical Activity And Sedentary Activity Patterns Among Children And Adolescents: A Latent Class Analysis Approach, Carrie D. Patnode, Leslie A. Lytle, Darin J. Erickson, John R. Sirard, Daheia J. Barr-Anderson, Mary Story

Faculty Publications

Background: While much is known about the overall levels of physical activity and sedentary activity among youth, few studies have attempted to define clusters of such behaviors. The purpose of this study was to identify and describe unique classes of youth based on their perception in a variety of physical activity and sedentary behaviors. Methods: Latent class analysis was used to characterize segments of youth based on patterns of self-reported and accelerometer-measured participation in 12 behaviors. Children and adolescents (N =720) from 6th-11th grade were included in the analysis. Differences in class membership were examined using multinomial logistic …

The Efficacy Of Dynamic Written Corrective Feedback For University Matriculated Esl Learners, Norman W. Evans, K. James Hartshorn, Diane Strong-Krause

Faculty Publications

Truscott’s (1996) indictment on error correction in second-language (L2) writing has ignited much discussion and research on the appropriateness of written corrective feedback (WCF) in L2 contexts. Out of this has emerged a body of research that suggests that WCF can positively impact the linguistic accuracy of student writing. However, these studies have examined only one or two error types. A central aspect of the efficacy of any corrective measure has to do with using feedback that is appropriate for the learner and the learning context. This paper builds on previous studies of dynamic WCF, which targets all linguistic errors …

Trends Over 5 Decades In U.S. Occupation-Related Physical Activity And Their Associations With Obesity, Timothy S. Church, Diana M. Thomas, Catrine Tudor-Locke, Peter T. Katzmarzyk, Conrad P. Earnest, Ruben Q. Rodarte, Corby K. Martin, Steven N. Blair, Claude Bouchard

Faculty Publications

Background: The true causes of the obesity epidemic are not well understood and there are few longitudinal population-based data published examining this issue. The objective of this analysis was to examine trends in occupational physical activity during the past 5 decades and explore how these trends relate to concurrent changes in body weight in the U.S.

Methodology/Principle Findings: Analysis of energy expenditure for occupations in U.S. private industry since 1960 using data from the U.S. Bureau of Labor Statistics. Mean body weight was derived from the U.S. National Health and Nutrition Examination Surveys (NHANES). In the early 1960's almost half …

Rex1/Zfp42 As An Epigenetic Regulator For Genomic Imprinting, Jeong Do Kim, Hana Kim, Muhammad B. Ekram, Sungryul Yu, Christopher Faulk, Joomyeong Kim

Faculty Publications

Zfp42/Rex1 (reduced expression gene 1) is a well-known stem-cell marker that has been duplicated from YY1 in the eutherian lineage. In the current study, we characterized the in vivo roles of Rex1 using a mutant mouse line disrupting its transcription. In contrast to the ubiquitous expression of YY1, Rex1 is expressed only during spermatogenesis and early embryogenesis and also in a very limited area of the placenta. Yet, the gene dosage of Rex1 is very critical for the survival of the late-stage embryos and neonates. This delayed phenotypic consequence suggests potential roles for Rex1 in establishing and maintaining unknown epigenetic …

Ratiometric Coumarin-Neutral Red (Coner) Nanoprobe For Detection Of Hydroxyl Radicals, Gabriela M. Ganea, Paulina E. Kolic, Bilal El-Zahab, Isiah M. Warner

Faculty Publications

Excessive production of reactive oxygen species can lead to alteration of cellular functions responsible for many diseases including cardiovascular diseases, neurodegenerative diseases, cancer, and aging. Hydroxyl radical is a short-lived radical which is considered very aggressive due to its high reactivity toward biological molecules. In this study, a COumarin-NEutral Red (CONER) nanoprobe was developed for detection of hydroxyl radical based on the ratiometric fluorescence signal between 7-hydroxy coumarin 3-carboxylic acid and neutral red dyes. Biocompatible poly lactide-co-glycolide (PLGA) nanoparticles containing encapsulated neutral red were produced using a coumarin 3-carboxylic acid conjugated poly(sodium N-undecylenyl-Nε-lysinate) (C3C-poly-Nε- SUK) as moiety reactive to hydroxyl …