Digital Commons Network^™

“A Space For Myself To Go:” Early Patterns In Small Ya Spaces, Anthony Bernier

Faculty Publications

While young adults (teenagers) are routinely recognized as constituting nearly 25 percent of the nation's public library users, the vast majority of libraries devote more space and design attention to restrooms than to young people. Worse, there are currently no consistent or established metrics, no evaluation criteria, few conceptual standards of best practices, and little consistency in the methods by which we collect empirical evidence about young adult (YA) spaces. This study is the first systematic attempt to both collect and analyze empirical data on libraries' recent trend toward providing greater spatial equity for YA library service.

Harmonious Federalism In Support Of National Energy Goals – Increased Wind Renewable Energy, Ronald H. Rosenberg

Faculty Publications

American energy policy has slowly begun to change the mix in the sources of supply of electricity to residences, industry, and businesses. Renewable sources of electricity have been promoted as future contributors of large portions of the nation's electricity consumption. Wind power has been identified as a potentially substantial future electricity source contributing up to 20% of American demand 2030. To achieve these optimistic goals, there must be: (1) cost-effective, reliable energy technology; (2) sufficient investment capital to finance new construction; and (3) the existence of supportive governmental policies at all levels government. This article discusses the importance of inter-governmental …

Revolutionary Decision, Meredith Jones-Gray

Faculty Publications

No abstract provided.

Phage-Induced Alignment Of Membrane Proteins Enables The Measurement And Structural Analysis Of Residual Dipolar Couplings With Dipolar Waves And Lambda-Maps, Sang Ho Park, Woo Sung Son, Rishi Mukhopadhyay, Homayoun Valafar, Stanley J. Opella

Faculty Publications

At pH > 6 added filamentous bacteriophage fd is compatible with many of the detergents used to solubilize membrane proteins for solution NMR studies of membrane proteins and, therefore, serves as an alignment media. In combination with strained polyacrylamide gel alignment, Dipolar Waves can be used to directly assess the secondary structure and a λ-map extracts the order tensors for de novo structure calculation of membrane proteins without distance restraints.

The Role Of Web Services In Portal Design: Approaches For An Algerian University Library, Mohamed Ridda Laouar, Richard Hacken, Mathew Miles

Faculty Publications

Purpose - Today, with access to enormous quantities of information facilitated by the web, boundaries between remote and local source documents become invisible. This is of extreme interest for libraries in Algeria, whose local holdings are very limited. Taking for inspiration the idea that a networked environment can benefit less affluent nations, we present a conceptual model for the design of a scholarly web portal at the University of Tébessa, with which we hope to create scholarly work stations that combine local and remote holdings, tools and documents.

Design/methodology/approach - We present an outlined conceptual model of library portal architecture, …

A Spectral Time-Domain Method For Computational Electrodynamics, James V. Lambers

Faculty Publications

We present a new approach to the numerical solution of Maxwell’s equations in the case of spatially‐varying electric permittivity and/or magnetic permeability, based on Krylov subspace spectral (KSS) methods. KSS methods for scalar equations compute each Fourier coefficient of the solution using techniques developed by Gene Golub and Gérard Meurant for approximating elements of functions of matrices by Gaussian quadrature in the spectral, rather than physical, domain. We show how they can be generalized to coupled systems of equations, such as Maxwell’s equations, by choosing appropriate basis functions that, while induced by this coupling, still allow efficient and robust computation …

High Efficiency Lipid-Based Sirna Transfection Of Adipocytes In Suspension, Gail Kilroy, David H. Burk, Z. Elizabeth Floyd

Faculty Publications

Background: Fully differentiated adipocytes are considered to be refractory to introduction of siRNA via lipid-based transfection. However, large scale siRNA-based loss-of-function screening of adipocytes using either electroporation or virally-mediated transfection approaches can be prohibitively complex and expensive. Methodology/Principal Findings: We present a method for introducing small interfering RNA (siRNA) into differentiated 3T3-L1 adipocytes and primary human adipocytes using an approach based on forming the siRNA/ cell complex with the adipocytes in suspension rather than as an adherent monolayer, a variation of "reverse transfection". Conclusions/Significance: Transfection of adipocytes with siRNA by this method is economical, highly efficient, has a simple workflow, …

From Pdf To Mp3: Motivations For Creating Derivative Works, John Hilton Iii

Faculty Publications

With increasing frequency, authors are licensing their works in such a way so as to permit others to create derivative works. In some cases, these derivatives extend the impact of a work by providing a translation into another language or modifying the file format to make it more accessible. The Internet is increasing the ability of individuals to create and distribute these derivatives. Seventeen creators of derivatives were surveyed on their motivations for doing so. They indicated that they were willing to create derivatives that extend the original content of a book because they want to help others access the …

Transcriptome Analysis Of Frog Virus 3, The Type Species Of The Genus Ranavirus, Family Iridoviridae, Sai Majji, V. Thodima, R. Sample, D. Whitley, Y. Deng, J. Mao, V. Gregory Chinchar

Faculty Publications

Frog virus 3 is the best characterized species within the genus Ranavirus, family Iridoviridae. FV3's large (similar to 105 kbp) dsDNA genome encodes 98 putative open reading frames (ORFs) that are expressed in a coordinated fashion leading to the sequential appearance of immediate early (IE), delayed early (DE) and late (L) viral transcripts. As a step toward elucidating molecular events in FV3 replication, we sought to identify the temporal Class Of Viral messages. To accomplish this objective an oligonucleotide microarray containing 70-mer probes corresponding to each of the 98 FV3 ORFs was designed and used to examine viral gene expression. …

Aall Review: Find 50-State Law Surveys Fast!, Michele Lucero

Faculty Publications

As a librarian, have you ever had the challenge of finding and compiling that needle-in-a-haystack 50-State survey? Or locating the resources to retrieve that subject survey quickly? What about a regulatory survey? Cheryl Rae Nyberg, of the University of Washington, Gallagher Law Library and author of Subject Compilation of State Laws (2006-07) An Annotated Bibliography, provided shortcuts and tips on how to quickly locate a 50-state law survey by any topic. This article is a brief summary of where a librarian can locate 50-state law surveys fast!

Aall Review: Beyond Copyright: How License Agreements & Digital Rights Management Post Challenges To Fair Use & The Provision Of Electronic Or Media Services, Michele Lucero

Faculty Publications

License agreements and Digital Rights Management (DRM) technologies are common among content suppliers. DRM is the use of technological means to control digital content. Librarians need to understand the connection between copyright and contract, and the implications of fair use.

Detecting Proteins In Highly Autofluorescent Cells Using Quantum Dot Antibody Conjugates, Karen M. Orcutt, Shanshan Ren, Kjell Gundersen

Faculty Publications

We have applied quantum dot (Qdot) antibody conjugates as a biomolecular probe for cellular proteins important in biogeochemical cycling in the sea. Conventional immunological methods have been hampered by the strong autofluorescence found in cyanobacteria cells. Qdot conjugates provide an ideal alternative for studies that require long-term imaging of cells such as detection of low abundance cellular antigens by fluorescence microscopy. The advantage of Qdot labeled probes over conventional immunological methods is the photostability of the probe. Phycoerythrin bleaches in cyanobacterial cells under prolonged UV or blue light excitation, which means that the semiconducting nanocrystal probe, the Qdot, can yield …

Book Review: The Internet And American Business, Eds. William Aspray And Paul E. Ceruzzi, Joseph A. November

Faculty Publications

No abstract provided.

Manipulating The Public Agenda: Why Acorn Was In The News, And What The News Got Wrong, Peter Dreier, Christopher R. Martin

Faculty Publications

This study, which received no outside funding from any organization, analyzed the complete 2007‐2008 coverage of ACORN by 15 major news media organizations, and the narrative frames of their 647 stories during that period. The news media analyzed include the four the highest circulation national newspapers—USA Today, New York Times, Washington Post, and the Wall Street Journal—and an analysis of the transcripts of reports from leading broadcast news organizations: ABC, CBS, NBC, Fox News Channel, CNN, MSNBC, National Public Radio (NPR), and NewsHour with Jim Lehrer (PBS). We also analyzed stories from three local newspapers …

Impediments To Learning Object Reuse And Openness As A Potential Solution, David Wiley

Faculty Publications

This paper has argued that the simple act of adding an open license providing 4Rs usage rights to learning object users will significantly expand our understanding of “reuse;” that the application of open licenses will greatly increase the reach of learning objects (making them available to individuals and organizations with financial or other resource challenges), and that open licenses are easy to apply to learning objects. Because of the challenges inherent with fully copyrighted learning objects, “open learning objects” seem to be the best path forward for this very promising educational technology.

Yy1 Is Autoregulated Through Its Own Dna-Binding Sites, Jeong D. Kim, Sungryul Yu, Joomyeong Kim

Faculty Publications

Background: The transcription factor Yin Yang 1 (YY1) is a ubiquitously expressed, multifunctional protein that controls a large number of genes and biological processes in vertebrates. As a general transcription factor, the proper levels of YY1 protein need to be maintained for the normal function of cells and organisms. However, the mechanism for the YY1 homeostasis is currently unknown. Results: The current study reports that the YY1 gene locus of all vertebrates contains a cluster of its own DNA-binding sites within the 1st intron. The intact structure of these DNA-binding sites is absolutely necessary for transcriptional activity of the YY1 …

Business Return In New Orleans: Decision Making Amid Post-Katrina Uncertainty, Nina S.N. Lam, Kelley Pace, Richard Campanella, James Lesage, Helbert Arenas

Faculty Publications

Background: Empirical observations on how businesses respond after a major catastrophe are rare, especially for a catastrophe as great as Hurricane Katrina, which hit New Orleans, Louisiana on August 29, 2005. We analyzed repeated telephone surveys of New Orleans businesses conducted in December 2005, June 2006, and October 2007 to understand factors that influenced decisions to re-open amid post-disaster uncertainty. Methodology/Principal Findings: Businesses in the group of professional, scientific, and technical services reopened the fastest in the near term, but differences in the rate of reopening for businesses stratified by type became indistinguishable in the longer term (around two years …

Mechanistic Investigation Of N-Homocysteinylation-Mediated Protein-Gold Nanoconjugate Assembly, Arther T. Gates, Leonard Moore, Monica R. Sylvain, Christina M. Jones, Mark Lowry, Bilal El-Zahab, James W. Robinson, Robert M. Strongin, Isiah M. Warner

Faculty Publications

Herein we report the use of protein-gold nanoconjugate (PGNs) as probes for elucidating mechanistic events involved in protein homocystamide detection with gold nanoparticles (GNPs), as was previously reported by our laboratory. Three different PGN probes are synthesized by direct adsorption of cytochrome c, albumin, or human serum onto citrate-capped GNPs. The PGNs are subsequently purified and treated to confer N-homocysteinylation. Individual PGN systems are evaluated to assess the effect of modification on (1) surface plasmon resonance (SPR), (2) protein structural conformation, and (3) assembly-association. The degree of PGN assembly and colorimetric signal observed postmodification varies based on the type of …

Metabolic Depression Is Delayed And Mitochondrial Impairment Averted During Prolonged Anoxia In The Ghost Shrimp, Lepidophthalmus Louisianensis (Schmitt, 1935), Jeremy D. Holman, Steven C. Hand

Faculty Publications

Lepidophthalmus louisianensis burrows deeply into oxygen-limited estuarine sediments and is subjected to extended anoxia at low tides. Large specimens (> 2 g) have a lethal time for 50% mortality (LT50) of 64 h under anoxia at 25 °C. Small specimens (< 1 g) have a significantly higher LT50 of 113 h, which is the longest ever reported for a crustacean. Whole body lactate levels rise dramatically under anoxia and exceed 120 μmol g.f.w.- 1 by 72 h. ATP, ADP, and AMP do not change during 48 h of anoxia, but arginine phosphate declines by over 50%. Thus arginine phosphate may help stabilize the ATP pool. Surprisingly, when compared to the aerobic resting rate, ATP production under anoxia declines only moderately during the first 12 h, and drops to only about 30% between 12 and 48 h. Finally, after 48 h of anoxia, a major metabolic depression to less than 5% occurs. Downregulation of metabolism is delayed in L. louisianensis compared to many invertebrates that exhibit facultative anaerobiosis. Bioenergetic constraints as a result of eventual metabolic depression lead to ionic disturbances like calcium overload and compromised membrane potential of mitochondria. Because these phenomena trigger apoptosis in mammalian species, we evaluated the susceptibility of ghost shrimp mitochondria to opening of the mitochondrial permeability transition pore (MPTP) and associated damage. Energized mitochondria isolated from hepatopancreas possess a pronounced capacity for calcium uptake. Exogenous calcium does not stimulate opening of the MPTP, which potentially could reduce cell death during prolonged anoxia. © 2009 Elsevier B.V. All rights reserved.

A Link Between Aurora Kinase And Clp1/Cdc14 Regulation Uncovered By The Identification Of A Fission Yeast Borealin-Like Protein, K. Adam Bohnert, Jun Song Chen, Dawn M. Clifford, Craig W. Vander Kooi, Kathleen L. Gould

Faculty Publications

The chromosomal passenger complex (CPC) regulates various events in cell division. This complex is composed of a catalytic subunit, Aurora B kinase, and three nonenzymatic subunits, INCENP, Survivin, and Borealin. Together, these four subunits interdependently regulate CPC function, and they are highly conserved among eukaryotes. However, a Borealin homologue has never been characterized in the fission yeast, Schizosaccharomyces pombe. Here, we isolate a previously uncharacterized S. pombe protein through association with the Cdc14 phosphatase homologue, Clp1/Flp1, and identify it as a Borealin-like member of the CPC. Nbl1 (novel Borealin-like 1) physically associates with known CPC components, affects the kinase activity …

Sexual Experience And Couple Formation Attitudes Among Emerging Adults, Brian J. Willoughby, Jason S. Carroll

Faculty Publications

In this study, we explore the relationship between attitudes toward both marriage and cohabitation and sexual experience during emerging adulthood. Results from 990 emerging adults indicated only moderate evidence that marital attitudes are related to sexual experience but strong evidence of a relationship between attitudes toward cohabitation and sexual experience. In particular, sexually active emerging adults were more likely to have positive attitudes toward cohabitation. Furthermore, it was found that both religiosity and dating status moderate the relationship between couple formation attitudes and sexual experience. For highly religious emerging adults, sexual activity was associated with higher endorsement of cohabitation; however, …

Enhancement Of Optics-To-Thz Conversion Efficiency By Metallic Slot Waveguides, Zhichao Ruan, Georgios Veronis, Konstantin L. Vodopyanov, Marty M. Fejer, Shanhui Fan

Faculty Publications

A metallic slot waveguide, with a dielectric strip embedded within, is investigated for the purpose of enhancing the optics-to-THz conversion efficiency using the difference-frequency generation (DFG) process. To describe the frequency conversion process in such lossy waveguides, a fully-vectorial coupled-mode theory is developed. Using the coupled-mode theory, we outline the basic theoretical requirements for efficient frequency conversion, which include the needs to achieve large coupling coefficients, phase matching, and low propagation loss for both the optical and THz waves. Following these requirements, a metallic waveguide is designed by considering the trade-off between modal confinement and propagation loss. Our numerical calculation …

Why Default On U.S. Treasuries Is Likely, Jeffrey Rogers Hummel

Faculty Publications

No abstract provided.

Intensity Dependence Of Laser-Assisted Attosecond Photoionization Spectra, M. Swoboda, J. M. Dahlström, T. Ruchon, P. Johnsson, J. Mauritsson, A. L'Huillier, K. J. Schafer

Faculty Publications

We study experimentally the influence of the intensity of the infrared (IR) probe field on attosecond pulse train (APT) phase measurements performed with the RABITT method (Reconstruction of Attosecond Beating by Interference in Two-Photon Transitions). We find that if a strong IR field is applied, the attosecond pulses will appear to have lower-than-actual chirp rates. We also observe the onset of the streaking regime in the breakdown of the weak-field RABITT conditions. We perform a Fourier-analysis of harmonic and sideband continuum states and show that the mutual phase relation of the harmonics can be extracted from higher Fourier components. © …

Inflation, Quantum Fields, And Cmb Anisotropies, Iván Agulló, José Navarro-Salas, Gonzalo J. Olmo, Leonard Parker

Faculty Publications

Inflationary cosmology has proved to be the most successful at predicting the properties of the anisotropies observed in the cosmic microwave background (CMB). In this essay we show that quantum field renormalization significantly influences the generation of primordial perturbations and hence the expected measurable imprint of cosmological inflation on the CMB. However, the new predictions remain in agreement with observation, and in fact favor the simplest forms of inflation. In the near future, observations of the influence of gravitational waves from the early universe on the CMB will test our new predictions. © 2009 Springer Science+Business Media, LLC.

Abstracting Gis Layers From Hyperspectral Imagery, Torsten E. Howard, Michael J. Mendenhall, Gilbert L. Peterson

Faculty Publications

The spectral-spatial relationship of materials in a hyperspectral image cube is exploited to partially automate the creation of Geographic Information System (GIS) layers. The topological neighborhood preservation property of the Self Organizing Map (SOM) is clustered into six (partially overlapping) neighborhoods that are mapped into the image domain to locate in-scene structures of similar material type. GIS layers are abstracted through spatial logical and morphological operations on the six image domain material maps and a novel road finding algorithm connects road segments under significant tree-occlusion resulting in a contiguous road network. It is assumed that specific knowledge of the scene …

A Trust-Based Multiagent System, Richard S. Seymour, Gilbert L. Peterson

Faculty Publications

Cooperative agent systems often do not account for sneaky agents who are willing to cooperate when the stakes are low and take selfish, greedy actions when the rewards rise. Trust modeling often focuses on identifying the appropriate trust level for the other agents in the environment and then using these levels to determine how to interact with each agent. Adding trust to an interactive partially observable Markov decision process (I-POMDP) allows trust levels to be continuously monitored and corrected enabling agents to make better decisions. The addition of trust modeling increases the decision process calculations, and solves more complex trust …

Positional Identification Of Variants Of Adamts16 Linked To Inherited Hypertension, Bina Joe, Yasser Saad, Norman H. Lee, Bryan C. Frank, Ovokeraye H. Achinike, Truong V. Luu, Kathirvel Gopalakrishnan, Edward J. Toland, Phyllis Farms, Shane Yerga-Woolwine, Ezhilarasi Manickavasagam, John P. Rapp, Michael R. Garrett, David Coe, Suneel S. Apte, Tuomo Rankinen, Louis Pérusse, Georg B. Ehret, Santhi K. Ganesh, Richard S. Cooper, Ashley O'Connor, Treva Rice, Alan B. Weder, Aravinda Chakravarti, Dabeeru C. Rao, Claude Bouchard

Faculty Publications

A previously reported blood pressure (BP) quantitative trait locus on rat Chromosome 1 was isolated in a short congenic segment spanning 804.6 kb. The 804.6 kb region contained only two genes, LOC306664 and LOC306665. LOC306664 is predicted to translate into A Disintegrin-like and Metalloproteinase with Thrombospondin Motifs-16 (Adamts16). LOC306665 is a novel gene. All predicted exons of both LOC306664 and LOC306665 were sequenced. Non-synonymous variants were identified in only one of these genes, LOC306664. These variants were naturally existing polymorphisms among inbred, outbred and wild rats. The full-length rat transcript of Adamts16 was detected in multiple tissues. Similar to ADAMTS16 …

Demetalation Of Silver(Iii) Corrolates, Manuela Stefanelli, Jing Shen, Weihua Zhu, Marco Mastroianni, Federica Mandoj, Sara Nardis, Zhongping Ou, Karl M. Kadiah, Frank R. Fronczek, Kevin M. Smith, Roberto Paolesse

Faculty Publications

Several procedures for the demetalation of silver(III) corrolates have been tested. Acidic conditions induce removal of the silver ion but they can also promote concomitant oxidation of the corroie nucleus to an isocorrole species, the degree of which will depend upon the specific acidic media. This oxidation cannot be completely avoided by addition of hydrazine, particularly In the case of 3-NO 2 substituted complexes which are quantitatively converted into the corresponding 3-NO 2,5-hydroxy isocorroles upon sliver ion removal. Several β-nitro isocorrole products were Isolated, and one was structurally characterized. Electrochemical and chemical reductive methods for silver(III) corrolates demetalation were then …

Energy Current Imaging Method For Time Reversal In Elastic Media, Brian E. Anderson, Michele Griffa, Robert A. Guyer, Paul A. Johnson, Carene Larmat, Pierre-Yves Le Bas, Timothy J. Ulrich

Faculty Publications

An energy current imaging method is presented for use in locating sources of wave energy during the back propagation stage of the time reversal process. During the back propagation phase of an ideal time reversal experiment, wave energy coalesces from all angles of incidence to recreate the source event; after the recreation, wave energy diverges in every direction. An energy current imaging method based on this convergence/divergence behavior has been developed. The energy current imaging method yields a smaller spatial distribution for source reconstruction than is possible with traditional energy imaging methods.