Digital Commons Network^™

Diversities In Virulence, Antifungal Activity, Pigmentation And Dna Fingerprint Among Strains Of Burkholderia Glumae, Hari S. Karki, Bishnu K. Shrestha, Jae Woo Han, Donald E. Groth, Inderjit K. Barphagha, Milton C. Rush, Rebecca A. Melanson, Beom Seok Kim, Jong Hyun Ham

Faculty Publications

Burkholderia glumae is the primary causal agent of bacterial panicle blight of rice. In this study, 11 naturally avirulent and nine virulent strains of B. glumae native to the southern United States were characterized in terms of virulence in rice and onion, toxofalvin production, antifungal activity, pigmentation and genomic structure. Virulence of B. glumae strains on rice panicles was highly correlated to virulence on onion bulb scales, suggesting that onion bulb can be a convenient alternative host system to efficiently determine the virulence of B. glumae strains. Production of toxoflavin, the phytotoxin that functions as a major virulence factor, was …

Dynamic Change Of Awareness During Meditation Techniques: Neural And Physiological Correlates, David Dillard-Wright

Faculty Publications

No abstract provided.

Atmospheric Organic Material And The Nutrients Nitrogen And Phosphorus It Carries To The Ocean, Maria Kanakidou, Robert A. Duce, Joseph M. Prospero, Alex R. Baker, Claudia R. Benitez-Nelson, Frank J. Dentener, Keith A. Hunter, Peter S. Liss, Natalie Mahowald, Gregory S. Okin, Manmohan Sarin, Kostas Tsigaridis, Mitsuo Uematsu, Lauren M. Zamora, Tong Zhu

Faculty Publications

[1] The global tropospheric budget of gaseous and particulate non‐methane organic matter (OM) is re‐examined to provide a holistic view of the role that OM plays in transporting the essential nutrients nitrogen and phosphorus to the ocean. A global 3‐dimensional chemistry‐transport model was used to construct the first global picture of atmospheric transport and deposition of the organic nitrogen (ON) and organic phosphorus (OP) that are associated with OM, focusing on the soluble fractions of these nutrients. Model simulations agree with observations within an order of magnitude. Depending on location, the observed water soluble ON fraction ranges from ∼3% to …

Mechanistic Insights Into Validoxylamine A 7'-Phosphate Synthesis By Vlde Using The Structure Of The Entire Product Complex, Michael C. Cavalier, Young Sun Yim, Shumpei Asamizu, David Neau, Khaled H. Almabruk, Taifo Mahmud, Yong Hwan Lee

Faculty Publications

The pseudo-glycosyltransferase VldE catalyzes non-glycosidic C-N coupling between an unsaturated cyclitol and a saturated aminocyclitol with the conservation of the stereochemical configuration of the substrates to form validoxylamine A 7′-phosphate, the biosynthetic precursor of the antibiotic validamycin A. To study the molecular basis of its mechanism, the three-dimensional structures of VldE from Streptomyces hygroscopicus subsp. limoneus was determined in apo form, in complex with GDP, in complex with GDP and validoxylamine A 7′-phosphate, and in complex with GDP and trehalose. The structure of VldE with the catalytic site in both an "open" and "closed" conformation is also described. With these …

Development Of Scn Connectivity And The Circadian Control Of Arousal: A Diminishing Role For Humoral Factors?, Andrew J. Gall, William D. Todd, Mark S. Blumberg

Faculty Publications

The suprachiasmatic nucleus (SCN) is part of a wake-promoting circuit comprising the dorsomedial hypothalamus (DMH) and locus coeruleus (LC). Although widely considered a "master clock," the SCN of adult rats is also sensitive to feedback regarding an animal's behavioral state. Interestingly, in rats at postnatal day (P)2, repeated arousing stimulation does not increase neural activation in the SCN, despite doing so in the LC and DMH. Here we show that, by P8, the SCN is activated by arousing stimulation and that selective destruction of LC terminals with DSP-4 blocks this activational effect. We next show that bidirectional projections among the …

Role Of Propionates In Substrate Binding To Heme Oxygenase From Neisseria Meningitidis: A Nuclear Magnetic Resonance Study, Dungeng Peng, Li Hua Ma, Kevin M. Smith, Xuhong Zhang, Michihiko Sato, Gerd N. La Mar

Faculty Publications

Heme oxygenase (HO) cleaves hemin into biliverdin, iron, and CO. For mammalian HOs, both native hemin propionates are required for substrate binding and activity. The HO from the pathogenic bacterium Neisseria meningitidis (NmHO) possesses a crystallographically undetected C-terminal fragment that by solution 1H nuclear magnetic resonance (NMR) is found to fold and interact with the active site. One of the substrate propionates has been proposed to form a salt bridge to the C-terminus rather than to the conventional buried cationic side chain in other HOs. Moreover, the C-terminal dipeptide Arg208His209 cleaves spontaneously over ∼24 h at a rate dependent on …

The Power Of Administrative Ethics: Moving Beyond Moralism And Instrumentalism, Ciarán O’Kelly, Melvin J. Dubnick

Faculty Publications

This paper makes the case for an approach to administrative ethics that regards it as a form of political power within contexts of organization and organizational change (reform). After articulating the logic of this perspective, it is applied to two cases of reform (No Child Left Behind in the US and National Health Service reform in the UK) where reformers have used the power of metrics and high-stakes accountability to force change — and in the process have altered the respective ethical landscapes.

Cost-Effective Asthma Treatments For Uninsured Or Underinsured Pediatric Patients, Karlen E. (Beth) Luthy, Emilianne Dougall, Renea L. Beckstrand

Faculty Publications

Nurse practitioners should constantly compare a medication's potential effect with its associated cost, thus identifying the most cost-effective treatment plan. Such an approach is warranted for both pediatric and adult patients. Prescribing medications for children who are uninsured or underinsured can be especially challenging, particularly for those patients diagnosed with a chronic health condition requiring routine treatment and follow-up. Asthma can be such a chronic illness for which the cost associated with treatment may become a barrier to compliance with the treatment plan. Cost-effective treatment options for asthma in the pediatric patient are presented.

Formation And Stabilization Of Combustion-Generated, Environmentally Persistent Radicals On Ni(Ii)O Supported On A Silica Surface, Eric Vejerano, Slawomir M. Lomnicki, Barry Dellinger

Faculty Publications

Previous studies have indicated environmentally persistent free radicals (EPFRs) are formed when hydroxyl- and chlorine-substituted aromatics chemisorbed on Cu(II)O and Fe(III)2O3 surfaces and were stabilized through their interactions with the surface metal cation. The current study reports our laboratory investigation on the formation and stabilization of EPFRs on a Ni(II)O surface. The EPFRs were produced by the chemisorption of adsorbates on the supported metal oxide surface and transfer of an electron from the adsorbate to the metal center, resulting in reduction of the metal cation. Depending on the temperature and the nature of the adsorbate, more than one type of …

The Potential Impact Of Plain Packaging Of Cigarette Products Among Brazilian Young Women: An Experimental Study, Christine M/ White, David Hammond, Jim Thrasher, Geoffrey T. Fong

Faculty Publications

Background: Tobacco use is responsible for 5.4 million deaths every year worldwide and is a leading cause of preventable death. The burden of these deaths is rapidly shifting to low and middle-income countries, such as Brazil. Brazil has prohibited most forms of tobacco advertising; however, the cigarette pack remains a primary source of marketing. The current study examined how tobacco packaging influences brand appeal and perceptions of health risk among young women in Brazil.

Methods: A between-subjects experiment was conducted in which 640 Brazilian women aged 16–26 participated in an online survey. Participants were randomized to view 10 cigarette packages …

Magnesium-Based Sacrificial Anode Cathodic Protection Coatings (Mg-Rich Primers) For Aluminum Alloys, Shashi S. Pathak, Sharathmukar K. Mendon, Michael D. Blanton, James W. Rawlins

Faculty Publications

Magnesium is electrochemically the most active metal employed in common structural alloys of iron and aluminum. Mg is widely used as a sacrificial anode to provide cathodic protection of underground and undersea metallic structures, ships, submarines, bridges, decks, aircraft and ground transportation systems. Following the same principle of utilizing Mg characteristics in engineering advantages in a decade-long successful R&D effort, Mg powder is now employed in organic coatings (termed as Mg-rich primers) as a sacrificial anode pigment to protect aerospace grade aluminum alloys against corrosion. Mg-rich primers have performed very well on aluminum alloys when compared against the current chromate …

Situated Peer Coaching And Unfolding Cases In The Fundamentals Skills Laboratory, Deborah O. Himes, Patricia K. Ravert

Faculty Publications

Using unfolding case studies and situated peer coaching for the Fundamentals Skills Laboratory provides students with individualized feedback and creates a realistic clinical learning experience. A quasi-experimental design with pre- and post-intervention data was used to evaluate changes in student ratings of the course. An instrument was used to examine students' self-ratings and student comments about each lab. We found that students' ratings of the lab remained high with the new method and self-evaluations of their performance were higher as the semester progressed. Students appreciated the personalized feedback associated with peer coaching and demonstrated strong motivation and self-regulation in learning. …

Situated Peer Coaching And Unfolding Cases In The Fundamentals Skills Laboratory, Deborah O. Himes, Patricia K. Ravert

Faculty Publications

Using unfolding case studies and situated peer coaching for the Fundamentals Skills Laboratory provides students with individualized feedback and creates a realistic clinical learning experience. A quasi-experimental design with pre- and post-intervention data was used to evaluate changes in student ratings of the course. An instrument was used to examine students' self-ratings and student comments about each lab. We found that students' ratings of the lab remained high with the new method and self-evaluations of their performance were higher as the semester progressed. Students appreciated the personalized feedback associated with peer coaching and demonstrated strong motivation and self-regulation in learning. …

The Impact Of Corruption On Firm Tax Compliance In Transition Economies: Whom Do You Trust?, Anna Alon, Amy M. Hageman

Faculty Publications

Tax compliance is an important issue for governments and the public alike. To meet public needs and fund public mandates, firms around the world are expected to comply with tax laws. Factors that are related to organizational (firm) tax compliance have not been sufficiently examined in the literature. Due to the increasing global influence of transition economies, factors associated with firm tax compliance in transition economies are particularly of interest. Based on a sample of over 5,000 firms from 22 former Soviet Bloc transition economies, we find that higher levels of corruption and higher levels of particularized trust (reliance on …

The Uncertainty Room: Strategies For Managing Uncertainty In A Surgical Waiting Room, Anne M. Stone, John C. Lammers

Faculty Publications

Objective: To describe experiences of uncertainty and management strategies for staff working with families in a hospital waiting room. Setting: A 288-bed nonprofit community hospital in a Midwestern city. Methods: Data were collected during individual, semi structured interviews with 3 volunteers, 3 technical staff members, and 1 circulating nurse (n=7), and during 40 hours of observation in a surgical waiting room. Interview transcripts were analyzed using constant comparative techniques. Results: The surgical waiting room represents the intersection of several sources of uncertainty that families experience. Findings also illustrate the ways in which staff manage the uncertainty of families in the …

The Ecology Of Vocation, Terri L. Elton

Faculty Publications

Those who care about the future of the church have a vested interest in both the quantity and the quality of candidates preparing for ministry in this generation and into the next. And it is easy to see those pastors as the product of a series of independent and individualized decisions. A college student, for example, meets with her pastor to discuss her future. Or an engineer sits at the kitchen table with his wife asking if they have the money for him to quit his job and head off to seminary. The future of ministry does indeed depend on …

Emotional Psychological And Related Problems Among Truant Youths: An Exploratory Latent Class Analysis, Richard Dembo, Rhissa Briones-Robinson, Rocío Aracelis Ungaro, Laura M. Gulledge, Lora M. Karas, Ken C. Winters, Steven Belenko, Paul Greenbaum

Faculty Publications

Latent class analysis was conducted on the psychosocial problems experienced by truant youths. Data were obtained from baseline interviews completed on 131 youths and their parents/guardians involved in a NIDA-funded, Brief Intervention Project. Results identified two classes of youths: Class 1(n=9) - youths with low levels of delinquency, mental health and substance abuse issues; and Class 2(n=37) - youths with high levels of these problems. Comparison of these two classes on their urine analysis test results and parent/guardian reports of traumatic events found significant (p<.05) differences between them that were consistent with their problem group classification. Our results have important implications for research and practice.

Adipogenesis, Kelesha Sarjeant, Jacqueline M. Stephens

Faculty Publications

Adipose tissue is an important site for lipid storage, energy homeostasis, and whole-body insulin sensitivity. It is important to understand the mechanisms involved in adipose tissue development and function, which can be regulated by the endocrine actions of various peptide and steroid hormones. Recent studies have revealed that white and brown adipocytes can be derived from distinct precursor cells. This review will focus on transcriptional control of adipogenesis and its regulation by several endocrine hormones. The general functions and cellular origins of adipose tissue and how the modulation of adipocyte development pertains to metabolic disease states will also be considered. …

Dnt1 Acts As A Mitotic Inhibitor Of The Spindle Checkpoint Protein Dma1 In Fission Yeast, Yamei Wang, Wen Zhu Li, Alyssa E. Johnson, Zhou Qing Luo, Xue Li Sun, Anna Feoktistova, W. Hayes Mcdonald, Ian Mcleod, John R. Yates, Kathleen L. Gould, Dannel Mccollum, Quan Wen Jin

Faculty Publications

The Schizosaccharomyces pombe checkpoint protein Dma1 couples mitotic progression with cytokinesis and is important in delaying mitotic exit and cytokinesis when kinetochores are not properly attached to the mitotic spindle. Dma1 is a ubiquitin ligase and potential functional relative of the human tumor suppressor Chfr. Dma1 delays mitotic exit and cytokinesis by ubiquitinating a scaffold protein (Sid4) of the septation initiation network, which, in turn, antagonizes the ability of the Polo-like kinase Plo1 to promote cell division. Here we identify Dnt1 as a Dma1-binding protein. Several lines of evidence indicate that Dnt1 inhibits Dma1 function during metaphase. First, Dnt1 interacts …

Sperm Cryopreservation In Live-Bearing Xiphophorus Fishes: Offspring Production From Xiphophorus Variatus And Strategies For Establishment Of Sperm Repositories, Huiping Yang, Rafael Cuevas-Uribe, Markita G. Savage, Ronald B. Walter, Terrence R. Tiersch

Faculty Publications

Cryopreservation of sperm from Xiphophorus fishes has produced live young in three species: X. hellerii, X. couchianus, and X. maculatus. In this study, the goal was to establish protocols for sperm cryopreservation and artificial insemination to produce live young in X. variatus, and to identify needs for repository development. The objectives were to: 1) collect basic biological characteristics of males; 2) cryopreserve sperm from X. variatus, 3) harvest live young from cryopreserved sperm, and 4) discuss the requirements for establishment of sperm repositories. The 35 males used in this study had a body weight of 0.298±0.096g (mean±SD), body length of …

Quaternary Ammonium Silane-Functionalized, Methacrylate Resin Composition With Antimicrobial Activities And Self-Repair Potential, Shi-Qiang Gong, Li-Na Niu, Lisa K. Kemp, Cynthia K.Y. Yiu, Heonjune Ryou, Yi-Pin Qi, John D. Blizzard, Sergey Nikonov, Martha G. Brackett, Regina L.W. Messer, Christine D. Wu, Jing Mao, L. Bryan Brister, Frederick A. Rueggeberg, Dwayne D. Arola, David H. Pashley, Franklin R. Tay

Faculty Publications

The design of antimicrobial polymers to address healthcare issues and minimize environmental problems is an important endeavor with both fundamental and practical implications. Quaternary ammonium silane-functionalized methacrylate (QAMS) represents an example of antimicrobial macromonomers synthesized by a sol–gel chemical route; these compounds possess flexible Si–O–Si bonds. In present work, a partially hydrolyzed QAMS co-polymerized with 2,2-[4(2-hydroxy 3-methacryloxypropoxy)-phenyl]propane is introduced. This methacrylate resin was shown to possess desirable mechanical properties with both a high degree of conversion and minimal polymerization shrinkage. The kill-on-contact microbiocidal activities of this resin were demonstrated using single-species biofilms of Streptococcus mutans (ATCC 36558), Actinomyces naeslundii (ATCC …

Kingly Authority: Is It Finding A Place In The Adventist Church?, Stan Patterson

Faculty Publications

No abstract provided.

Oncology Nurses' Obstacles And Supportive Behaviors In End-Of-Life Care: Providing Vital Family Care, Renea L. Beckstrand, Joan Collette, Lynn Callister, Karlen E. (Beth) Luthy

Faculty Publications

Purpose/Objectives: To determine the impact of obstacles and supportive behaviors in end-of-life (EOL) care as perceived by hospital-based oncology nurses.

Design: A 69-item mailed survey.

Setting: National random sample.

Sample: 1,005 nurse members of the Oncology Nursing Society who had provided EOL care for patients with cancer.

Methods: Three mailings yielded 380 usable responses from 912 eligible respondents, resulting in a 42% return rate.

Main Research Variables: Size and frequency of EOL care obstacles and supportive behaviors for patients with cancer in a hospital setting.

Findings: Results of this research demonstrate the need for more EOL education and help in …

A Novel Allosteric Inhibitor Of Macrophage Migration Inhibitory Factor (Mif), Fengwei Bai, Oluwatoyin A. Asojo, Pier Cirillo, Mihai Ciustea, Michel Ledzidet, Paul A. Aristoff, Lin Leng, Raymond A. Koski, Thomas J. Powell, Richard Bucala, Karen G. Anthony

Faculty Publications

Macrophage migration inhibitory factor (MIF) is a catalytic cytokine and an upstream mediator of the inflammatory pathway. MIF has broad regulatory properties, dysregulation of which has been implicated in the pathology of multiple immunological diseases. Inhibition of MIF activity with small molecules has proven beneficial in a number of disease models. Known small molecule MIF inhibitors typically bind in the tautomerase site of the MIF trimer, often covalently modifying the catalytic proline. Allosteric MIF inhibitors, particularly those that associate with the protein by noncovalent interactions, could reveal novel ways to block MIF activity for therapeutic benefit and serve as chemical …

Database Of Diazotrophs In Global Ocean: Abundance, Biomass And Nitrogen Fixation Rates, Y.-W. Luo, S.C. Doney, L.A. Anderson, M. Benavides, I. Berman-Frank, A. Bode, S. Bonnet, K. H. Boström, D. Böttjer, D.G. Capone, E.J. Carpenter, Y.L. Chen, M.J. Church, J.E. Dore, L.I. Falcón, A. Fernández, R.A. Foster, K. Furuya, F. Gómez, K. Gundersen, A.M. Hynes, D.M. Karl, S. Kitajima, R.J. Langlois, J. Laroche, R.M. Letelier, E. Marañón, D.J. Mcgillicuddy Jr., P.H. Moisander, C.M. Moore, B. Mouriño-Carballido, M.R. Mulholland, J.A. Needoba, K.M. Orcutt, A.J. Poulton, E. Rahav, P. Raimbault, A.P. Rees, L. Riemann, T. Shiozaki, A. Subramaniam, T. Tyrell, K.A. Turk-Kubo, M. Varela, T.A. Villareal, E.A. Webb, A.E. White, J. Wu, J.P. Zehr

Faculty Publications

Marine N₂ fixing microorganisms, termed diazotrophs, are a key functional group in marine pelagic ecosystems. The biological fixation of dinitrogen (N₂) to bioavailable nitrogen provides an important new source of nitrogen for pelagic marine ecosystems and influences primary productivity and organic matter export to the deep ocean. As one of a series of efforts to collect biomass and rates specific to different phytoplankton functional groups, we have constructed a database on diazotrophic organisms in the global pelagic upper ocean by compiling about 12 000 direct field measurements of cyanobacterial diazotroph abundances (based on microscopic cell counts or …

Opening Our Anthropological Conversations: An Interview With Tom Boellstorff, Ryan B. Anderson

Faculty Publications

I had the chance to conduct an email-based interview with Tom Boellstorff during this past month to explore some of his views about Open Access (hereafter OA) publishing in anthropology.

Ryan Anderson: First of all, thanks for taking the time to do this interview, Tom. Here at Savage Minds we write about Open Access (OA) a lot, and many of our contributors seem to be in agreement about the need to look into alternative publishing options. But not everyone knows about OA or is in agreement with the push to head in that direction, and this includes many people who …

Current Status Of Boron Neutron Capture Therapy Of High Grade Gliomas And Recurrent Head And Neck Cancer, Rolf F. Barth, M. Graca H. Vicente, Otto K. Harling, W. S. Kiger, Kent J. Riley, Peter J. Binns, Franz M. Wagner, Minoru Suzuki, Teruhito Aihara, Itsuro Kato, Shinji Kawabata

Faculty Publications

Boron neutron capture therapy (BNCT) is a biochemically targeted radiotherapy based on the nuclear capture and fission reactions that occur when non-radioactive boron-10, which is a constituent of natural elemental boron, is irradiated with low energy thermal neutrons to yield high linear energy transfer alpha particles and recoiling lithium-7 nuclei. Clinical interest in BNCT has focused primarily on the treatment of high grade gliomas, recurrent cancers of the head and neck region and either primary or metastatic melanoma. Neutron sources for BNCT currently have been limited to specially modified nuclear reactors, which are or until the recent Japanese natural disaster, …

Social Communication And Theory Of Mind In Boys With Autism And Fragile X Syndrome, Jessica Klusek

Faculty Publications

Impairments in the social use of language, or pragmatics, constitute a core characteristic of autism. Problems with pragmatic language have also been documented in fragile X syndrome (FXS), a monogenic condition that is the most common known genetic cause of autism. Evidence suggests that social cognitive ability, or theory of mind, may also be impaired in both conditions, and in autism, may importantly relate to pragmatic language ability. Given the substantial overlap observed in autism and FXS, this study aimed to better define those social-communicative phenotypes that overlap in these two conditions by comparing pragmatic language ability and theory of …

Social Communication And Theory Of Mind In Boys With Autism And Fragile X Syndrome, Molly Losh, Gary E. Martin, Jessica Klusek, Abigail L. Hogan-Brown, John Sideris

Faculty Publications

Impairments in the social use of language, or pragmatics, constitute a core characteristic of autism. Problems with pragmatic language have also been documented in fragile X syndrome (FXS), a monogenic condition that is the most common known genetic cause of autism. Evidence suggests that social cognitive ability, or theory of mind, may also be impaired in both conditions, and in autism, may importantly relate to pragmatic language ability. Given the substantial overlap observed in autism and FXS, this study aimed to better define those social-communicative phenotypes that overlap in these two conditions by comparing pragmatic language ability and theory of …

The Structure Of The Bfrb-Bfd Complex Reveals Protein-Protein Interactions Enabling Iron Release From Bacterioferritin, Huili Yao, Yan Wang, Scott Lovell, Ritesh Kumar, Anatoly M. Ruvinsky, Kevin P. Battaile, Ilya A. Vakser, Mario Rivera

Faculty Publications

Ferritin-like molecules are unique to cellular iron homeostasis because they can store iron at concentrations much higher than those dictated by the solubility of Fe3+. Very little is known about the protein interactions that deliver iron for storage or promote the mobilization of stored iron from ferritin-like molecules. Here, we report the X-ray crystal structure of Pseudomonas aeruginosa bacterioferritin (Pa-BfrB) in complex with bacterioferritin-associated ferredoxin (Pa-Bfd) at 2.0 Å resolution. As the first example of a ferritin-like molecule in complex with a cognate partner, the structure provides unprecedented insight into the complementary interface that enables the [2Fe-2S] cluster of Pa-Bfd …