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Genomic Analysis For Virulence Determinants In Feline Herpesvirus Type-1 Isolates, Andrew C. Lewin, Lyndon M. Coghill, Gillian J. Mclellan, Ellison Bentley, Konstantin G. Kousoulas

Faculty Publications

© 2019, Springer Science+Business Media, LLC, part of Springer Nature. Feline herpesvirus type 1 (FHV-1) is a widespread cause of respiratory and ocular disease in domestic cats. A spectrum of disease severity is observed in host animals, but there has been limited prior investigation into viral genome factors which could be responsible. Stocks of FHV-1 were established from oropharyngeal swabs obtained from twenty-five cats with signs of infection housed in eight animal shelters around the USA. A standardized numerical host clinical disease severity scoring scheme was used for each cat from which an isolate was obtained. Illumina MiSeq was used …

Intramuscular Vaccination Of Mice With The Human Herpes Simplex Virus Type-1(Hsv-1) Vc2 Vaccine, But Not Its Parental Strain Hsv-1 (F) Confers Full Protection Against Lethal Ocular Hsv-1 (Mckrae) Pathogenesis, Shan K. Naidu, Rafiq Nabi, Nagarjuna R. Cheemarla, Brent A. Stanfield, Paul J. Rider, Nithya Jambunathan, Vladimir N. Chouljenko, Renee Carter, Fabio Del Piero, Ingeborg Langohr, Konstantin G. Kousoulas

Faculty Publications

Copyright: © 2020 Naidu et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Herpes simplex virus type-1 (HSV-1) can cause severe ocular infection and blindness. We have previously shown that the HSV-1 VC2 vaccine strain is protective in mice and guinea pigs against genital herpes infection following vaginal challenge with HSV-1 or HSV-2. In this study, we evaluated the efficacy of VC2 intramuscular vaccination in mice against herpetic keratitis following ocular challenge with …

Perinatal Maternal Antibiotic Exposure Augments Lung Injury In Offspring In Experimental Bronchopulmonary Dysplasia, Kent A. Willis, David T. Siefker, Michael M. Aziz, Catrina T. White, Naiha Mussarat, Charles K. Gomes, Amandeep Bajwa, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati

Faculty Publications

Copyright © 2020 the American Physiological Society. During the newborn period, intestinal commensal bacteria influence pulmonary mucosal immunology via the gut-lung axis. Epidemiological studies have linked perinatal antibiotic exposure in human newborns to an increased risk for bronchopulmonary dysplasia, but whether this effect is mediated by the gut-lung axis is unknown. To explore antibiotic disruption of the newborn gut-lung axis, we studied how perinatal maternal antibiotic exposure influenced lung injury in a hyperoxia-based mouse model of bronchopulmonary dysplasia. We report that disruption of intestinal commensal colonization during the perinatal period promotes a more severe bronchopulmonary dysplasia phenotype characterized by increased …

Mice Without A Microbiome Are Partially Protected From Lung Injury By Hyperoxia, Kent A. Willis, Joseph F. Pierre, Stephania A. Cormier, Ajay J. Talati

Faculty Publications

No abstract provided.

Quartz Crystal Microbalance Based Sensor Arrays For Detection And Discrimination Of Vocs Using Phosphonium Ionic Liquid Composites, Stephanie R. Vaughan, Rocío L. Pérez, Pratap Chhotaray, Isiah M. Warner

Faculty Publications

© 2020 by the authors. Licensee MDPI, Basel, Switzerland. Herein, we examine two sensing schemes for detection and discrimination of chlorinated volatile organic compounds (VOCs). In this work, phosphonium ionic liquids (ILs) were synthesized and vapor sensing properties examined and compared to phosphonium IL‐polymer composites. Pure IL sensors were used to develop a QCM‐based multisensory array (MSA), while IL‐polymer composites were used to develop an MSA and virtual sensor arrays (VSAs). It was found that by employing the composite MSA, five chlorinated VOCs were accurately discriminated at 95.56%, which was an increase in accuracy as compared to pure ILs MSA …

Numerical Experiments On Variation Of Freshwater Plume And Leakage Effect From Mississippi River Diversion In The Lake Pontchartrain Estuary, Wei Huang, Chunyan Li, John R. White, Sibel Bargu, Brian Milan, Sam Bentley

Faculty Publications

©2020. American Geophysical Union. All Rights Reserved. A Finite Volume Community Ocean Model is used to investigate how wind impacts the circulation and evolution of a freshwater plume from Mississippi River diversion in the Lake Pontchartrain Estuary. Results show that northerly and southerly winds tend to stretch the plume in the east-west directions, while easterly and westerly winds constrain the plume in the north-south directions. Increasing wind magnitude tends to increase the total salt content of the estuary except under weak westerly wind (/s) during which salt content decreases. A no-motion middepth interface is found (by the model and verified …

Respiratory Syncytial Virus Disease Severity Is Associated With Distinct Cd8+ T-Cell Profiles, David T. Siefker, Luan Vu, Dahui You, Andrew Mcbride, Ryleigh Taylor, Tamekia L. Jones, John Devincenzo, Stephania A. Cormier

Faculty Publications

Copyright © 2020 by the American Thoracic Society. Rationale: Respiratory syncytial virus (RSV) causes significant morbidity and mortality in infants worldwide. Although T-helper type 2 (Th2) cell pathology is implicated in severe disease, the mechanisms underlying the development of immunopathology are incompletely understood. Objectives: We aimed to identify local immune responses associated with severe RSV in infants. Our hypothesis was that disease severity would correlate with enhanced Th2 cellular responses. Methods: Nasal aspirates were collected from infants hospitalized with severe (admitted to the pediatric ICU) or moderate (maintained in the general ward) RSV disease at 5 to 9 days after …

Hot-Water Baths, Biologicals And Re-Curing Effects On Rhizopus Soft Rot During Sweetpotato Packing, R. R. Sweany, D. H. Picha, C. A. Clark

Faculty Publications

Rhizopus soft rot (RSR) caused by Rhizopus stolonifer is one of the most devastating postharvest diseases of sweetpotato. It causes greatest losses when sweetpotatoes are removed from storage, washed and packed for marketing. The disease has been managed effectively by prophylactic application of synthetic fungicides on the packing line. However, there is increasing demand for alternative management strategies that do not rely on prophylactic use of synthetic fungicides. While curing immediately after harvest is a standard industry practice, re-curing after storage is not widely practised for postharvest disease management. In this study, the use of hot-water baths, biocontrol agents and …

Maize Yield In Smallholder Agriculture System-An Approach Integrating Socioeconomic And Crop Management Factors, Sudarshan Dutta, Somsubhra Chakraborty, Rupak Goswami, Hirak Banerjee, Kaushik Majumdar, Bin Li, M. L. Jat

Faculty Publications

Yield gaps of maize (Zea mays L.) in the smallholder farms of eastern India are outcomes of a complex interplay of climatic variations, soil fertility gradients, socio-economic factors, and differential management intensities. Several machine learning approaches were used in this study to investigate the relative influences of multiple biophysical, socio-economic, and crop management features in determining maize yield variability using several machine learning approaches. Soil fertility status was assessed in 180 farms and paired with the surveyed data on maize yield, socio-economic conditions, and agronomic management. The C&RT relative variable importance plot identified farm size, total labor, soil factors, seed …

Ceramide–Rubusoside Nanomicelles, A Potential Therapeutic Approach To Target Cancers Carrying P53 Missense Mutations, Sachin K. Khiste, Zhijun Liu, Kartik R. Roy, Mohammad B. Uddin, Salman B. Hosain, Xin Gu, Sami Nazzal, Ronald A. Hill, Yong Yu Liu

Faculty Publications

Ceramide (Cer) is an active cellular sphingolipid that can induce apoptosis or proliferation-arrest of cancer cells. Nanoparticle-based delivery offers an effective approach for overcoming bioavailability and biopharmaceutics issues attributable to the pronounced hydrophobicity of Cer. Missense mutations of the protein p53, which have been detected in approximately 42% of cancer cases, not only lose the tumor suppression activity of wild-type p53, but also gain oncogenic functions promoting tumor progression and drug resistance. Our previous works showed that cellular Cer can eradicate cancer cells that carry a p53 deletion-mutation by modulating alternative pre-mRNA splicing, restoring wild-type p53 protein expression. Here, we …

Self-Rated Religiosity/Spirituality And Four Health Outcomes Among Us South Asians: Findings From The Study On Stress, Spirituality, And Health, Samuel Stroope, Blake Victor Kent, Ying Zhang, Namratha R. Kandula, Alka M. Kanaya, Alexandra E. Shields

Faculty Publications

Almost no research exists on the relationship between religiosity/spirituality (R/S) and health in the US South Asian population. Using data from the joint Study on Stress, Spirituality, and Health and Mediators of Atherosclerosis Among South Asians Living in America Study (MASALA), this article examined associations between self-rated R/S and self-rated health, emotional functioning, trait anxiety, and trait anger in a community-based sample (n = 933) from the Chicago and San Francisco Bay areas. Ordinary least squares regression was used to analyze categorical differences in levels of R/S and ordinal trends for R/S, adjusting for potential confounders. Being slightly or moderately …

Trans-Disciplinary Responses To Climate Change: Lessons From Rice-Based Systems In Asia, Jon Hellin, Jean Balié, Eleanor Fisher, Ajay Kohli, Melanie Connor, Sudhir Yadav, Virender Kumar, Timothy J. Krupnik, Bjoern Ole Sander, Joshua Cobb, Katherine Nelson, Tri Setiyono, Ranjitha Puskur, Pauline Chivenge, Martin Gummert

Faculty Publications

Climate change will continue to have a largely detrimental impact on the agricultural sector worldwide because of predicted rising temperatures, variable rainfall, and an increase in extreme weather events. Reduced crop yields will lead to higher food prices and increased hardship for low income populations, especially in urban areas. Action on climate change is one of the Sustainable Development Goals (SDG 13) and is linked to the Paris Climate Agreement. The research challenge posed by climate change is so complex that a trans-disciplinary response is required, one that brings together researchers, practitioners, and policy-makers in networks where the lines between …

Evaluation Of Ultraviolet (Uv-C) Light Treatment For Microbial Inactivation In Agricultural Waters With Different Levels Of Turbidity, Achyut Adhikari, Katheryn J. Parraga Estrada, Vijay S. Chhetri, Marlene Janes, Kathryn Fontenot, John C. Beaulieu

Faculty Publications

Produce growers using surface or well water to irrigate their crops may require an appropriate water treatment system in place to meet the water quality standard imposed by FSMA Produce Safety Rule. This study evaluated the potential of using ultraviolet (UV-C) treatment in reducing the microbial population in agricultural water. Waters with turbidity levels ranging from 10.93 to 23.32 Nephelometric Turbidity Units (NTU) were prepared by mixing pond water and well water. The waters were inoculated with a cocktail of generic Escherichia coli (ATCC 23716, 25922, and 11775) and then treated with UV-C light (20–60 mJ/cm2). All tested doses of …

On The Usefulness Of Parental Lines Gwas For Predicting Low Heritability Traits In Tropical Maize Hybrids, Giovanni Galli, Filipe Couto Alves, Júlia Silva Morosini, Roberto Fritsche-Neto

Faculty Publications

Genome-wide association studies (GWAS) is one of the most popular methods of studying the genetic control of traits. This methodology has been intensely performed on inbred genotypes to identify causal variants. Nonetheless, the lack of covariance between the phenotype of inbred lines and their offspring in cross-pollinated species (such as maize) raises questions on the applicability of these findings in a hybrid breeding context. To address this topic, we incorporated previously reported parental lines GWAS information into the prediction of a low heritability trait in hybrids. This was done by marker-assisted selection based on significant markers identified in the lines …

Agricultural And Finance Intervention Increased Dietary Intake And Weight Of Children Living In Hiv-Affected Households In Western Kenya, Lisa M. Butler, Shiva Bhandari, Phelgona Otieno, Sheri D. Weiser, Craig R. Cohen, Edward A. Frongillo Jr.

Faculty Publications

We tested whether a multisectoral household agricultural and finance intervention increased the dietary intake and improved the nutritional status of HIV-affected children. Two hospitals in rural Kenya were randomly assigned to be either the intervention or the control arm. The intervention comprised a human-powered water pump, microfinance loan for farm commodities, and training in sustainable farming practices and financial management. In each arm, 100 children (0-59 mo of age) were enrolled from households with HIV-infected adults 18-49 y old. Children were assessed beginning in April 2012 and every 3 mo for 1 y for dietary intake and anthropometry. Children in …

Improving Construction Work Zone Safety Using Technology: A Systematic Review Of Applicable Technologies, Chukwuma Nnaji, John Gambatese, Hyun Woo Lee, Fan Zhang

Faculty Publications

© 2019 The Authors Once considered conventional, the construction industry is gradually increasing its reliance on innovations such as the application of technologies in safety management. Given the growing literature on technology applications in safety management and the varying opinions on the utility of applied technologies, a systematic review that streamlines findings from past studies is indispensable to construction stakeholders. Although a number of review studies are available in the building construction sector, the level of fragmentation and uniqueness within the construction industry necessitates a review study specifically targeting the heavy civil sector. In response, the present study applies a …

Disruptive Innovation: Impact For Practice, Policy, And Academia, Heather V. Nelson-Brantley, K. David Bailey, Joyce Batcheller, Laura Caramanica, Bret Lyman, Francine Snow

Faculty Publications

The 2019 Association for Leadership Science in Nursing International Conference, Disruptive Innovation, was held in Los Angeles, California, with attendees from 30 US States, Canada, Brazil, and China. Presenters discussed the need for nurse leaders to advocate for health equity, lead evidence-based innovation, how robots and other technology are generating disruptive innovations in healthcare, and building strong academic-practice partnerships to address nursing workforce challenges. This article will report on these important insights.

Functional And Transcriptomic Characterization Of Cisplatin-Resistant Ags And Mkn-28 Gastric Cancer Cell Lines, Barbara Mora-Lagos, Irene Cartas-Espinel, Ismael Riquelme, Alyssa C. Parker, Stephen R. Piccolo, Tamara Viscarra, Maria Elena Reyes, Louise Zanella, Kurt Buchegger, Carmen Ili, Priscilla Brebi

Faculty Publications

Gastric cancer (GC) is a significant cancer-related cause of death worldwide. The most used chemotherapeutic regimen in GC is based on platinum drugs such as cisplatin (CDDP). However, CDDP resistance reduces advanced GC survival. In vitro drug-resistant cell model would help in the understanding of molecular mechanisms underlying this drug-resistance phenomenon. The aim of this study was to characterize new models of CDDP-resistant GC cell lines (AGS R-CDDP and MKN-28 R-CDDP) obtained through a stepwise increasing drug doses method, in order to understand the molecular mechanisms underlying chemoresistance as well as identify new therapeutic targets for the treatment of GC. …

A Transcriptional Regulatory System Of The S. Cerevisiae Ole1 Gene Responds To Fatty Acid Species And Intracellular Amount, And Not Simply Membrane Status, Marshall Willey, Matthew Ochs, Clara Schriemer, Virginia Mcdonough

Faculty Publications

We examined the effects of unsaturated fatty acid (UFA) species and their concentration on the expression of OLE1, which encodes the stearoyl CoA desaturase, in Saccharomyces cerevisiae. We controlled the amount of UFA taken up by the cell by varying the concentration of tergitol in the medium. When cultured with 1 mM fatty acid in 0.1% tergitol, cells took up much more fatty acid than when cultured with the same concentration of fatty acid at 1% tergitol, although the amount incorporated was dependent on UFA species. For each fatty acid tested, we found that the higher uptake (0.1% tergitol condition) …

Acquisition And Development Of Verb/Predicate Chaining In Hebrew, Ruth Aronson Berman, Lyle Lustigman

Faculty Publications

The study considers development and use of verb/predicate chaining constructions by Hebrew speakers from early childhood to adolescence, based on analysis of authentic conversational and narrative corpora. Three types of constructions are analyzed, ordered hierarchically by degree of cohesivity and obligatoriness of chaining: (1) monoclausal complex predicates (the “extended predicates” of traditional Hebrew grammars); (2) coreferential interclausal predicate chaining; and (3) discursively motivated topic chaining. Relevant typological features of Modern Hebrew are reviewed as accounting for the absence of canonical clause chaining in the language (the paucity of non-finite constructions in everyday usage, absence of an uninflected basic form of …

Childhood Adversity, Mental Health, And The Perpetration Of Physical Violence In The Adult Intimate Relationships Of Women Prisoners: A Life Course Approach, Melissa S. Jones, Stephanie W. Burge, Susan F. Sharp, David A. Mcleod

Faculty Publications

Background: Adverse childhood experiences (ACEs) are common, with nearly two-thirds of adult samples reporting exposure to at least one and one-quarter reporting exposure to three or more distinct types of ACEs. ACEs have been linked to various negative outcomes across the life course, including mental health problems, and the perpetration of physical violence in intimate relationships. However, little is known about the relationships between ACEs, PTSD symptomology, and use of physical violence against an adult intimate partner among incarcerated women.

Objective: The purpose of this paper is to examine the relationships between ACEs, PTSD symptoms, and the perpetration of the …

Carbon Use Efficiency And Its Temperature Sensitivity Covary In Soil Bacteria, Grace Pold, Luiz A. Domeignoz-Horta, Eric W. Morrison, Serita D. Frey, Seeta A. Sistla, Kristen M. Deangelis

Faculty Publications

The strategy that microbial decomposers take with respect to using substrate for growth versus maintenance is one essential biological determinant of the propensity of carbon to remain in soil. To quantify the environmental sensitivity of this key physiological trade-off, we characterized the carbon use efficiency (CUE) of 23 soil bacterial isolates across seven phyla at three temperatures and with up to four substrates. Temperature altered CUE in both an isolate-specific manner and a substrate-specific manner. We searched for genes correlated with the temperature sensitivity of CUE on glucose and deemed those functional genes which were similarly correlated with CUE on …

Trans-European Division Report: Global Church Member Survey 2017-2018, Petr A. Činčala, Shannon M. Trecartin, Audrey Andersson

Faculty Publications

This report is the first examination of the Trans-European Division (TED) 2017-2018 Global Church Member Survey (GCMS) data. The research team worked closely with the metaanalysis team to ensure consistent implementation of the research protocol and consistent use of the questionnaire. A total of 1331 surveys were used from all TED Unions. The findings of this document are an initial analysis of the data using the latest version of SPSS. The purpose of the Executive Summary is to highlight key findings from the study, which includes: 1. Summarizing the demographic findings in order to describe the characteristics of church members …

North American Division: Report Global Church Member Survey 2017-2018, Petr A. Činčala

Faculty Publications

This report is the first examination of the North American Division 2017-2018 Global Church Member Survey (GCMS) data. The research team worked closely with the metaanalysis team to ensure consistent implementation of the research protocol and consistent use of the questionnaire. A total of 1,923 surveys were used from all NAD unions. The findings of this document are an initial analysis of the data using the latest version of SPSS. The purpose of the Executive Summary is to highlight key findings from the study, which includes: • Summarizing the demographic findings in order to describe the characteristics of church members …

Northern-Asian Pacific Division Report: Global Church Member Survey 2017-2018, Petr A. Činčala, Shannon M. Trecartin, Gustavo Emanoel, Christie Chui-Shan Chow

Faculty Publications

This report is the first examination of the Northern-Asian Pacific Division 2017-2018 Global Church Member Survey (GCMS) data. The research team worked closely with the metaanalysis team to ensure consistent implementation of the research protocol and consistent use of the questionnaire. A total of 3064 surveys were used from all NSD Unions. The findings of this document are an initial analysis of the data using the latest version of SPSS. The purpose of the Executive Summary is to highlight key findings from the study, which includes: 1. Summarizing the demographic findings in order to describe the characteristics of church members …

Euro-Asian Division Report: Global Church Member Survey 2017-2018, Petr A. Činčala, Pavel Zubkov, Dmitry Fokin, Shannon M. Trecartin

Faculty Publications

This report is the first examination of the Euro-Asian Division 2017-2018 Global Church Member Survey (GCMS) data. The research team worked closely with the meta-analysis team to ensure consistent implementation of the research protocol and consistent use of the questionnaire. A total of 2251 surveys were used from all ESD Unions. The findings of this document are an initial analysis of the data using the latest version of SPSS. The purpose of the Executive Summary is to highlight key findings from the study, which includes: 1. Summarizing the demographic findings in order to describe the characteristics of church members in …

Southern Asia Division Report: Global Church Member Survey 2017-2018, Petr A. Činčala, Shannon M. Trecartin, Vara Deepati

Faculty Publications

This report is the first examination of the Southern Asia Division 2017-2018 Global Church Member Survey (GCMS) data. The research team worked closely with the meta-analysis team to ensure consistent implementation of the research protocol and consistent use of the questionnaire. A total of 2981 surveys were used from all SUD Unions. The findings of this document are an initial analysis of the data using the latest version of SPSS. The purpose of the Executive Summary is to highlight key findings from the study, which includes: 1. Summarizing the demographic findings in order to describe the characteristics of church members …

Veteran Suicide Risk Factors: A National Sample Of Nonveteran And Veteran Men Who Died By Suicide, David S. Wood, Bethany M. Wood, Aislinn Watson, Devan Sheffield, Helena Hauter

Faculty Publications

Veteran suicide is a serious public health problem. Some data suggest that veteran suicide risk profiles differ from those of nonveterans. Records for veteran (n = 21,692) and nonveteran (n = 83,430) men who died by suicide were examined from 17 U.S. states using the National Violent Death Reporting System data. Seventeen precipitating factors were examined and combined through meta-analysis of proportions. Many precipitating factors were found to be less frequent for veterans. A smaller number of factors were found to be higher in the veteran population, including physical health problems. A sizable cumulative effect size (1.02) was observed, suggesting …

Influence Of Neighboring Clonal-Colonies On Aflatoxin Production By Aspergillus Flavus, Rebecca R. Sweany, Kenneth E. Damann

Faculty Publications

Aspergillus flavus is an ascomycete fungus that infects and contaminates corn, peanuts, cottonseed, and treenuts with acutely toxic and carcinogenic aflatoxins. The ecological function of aflatoxin production is not well understood; though not phytotoxic, aflatoxin may be involved in resisting oxidative stress responses from infection or drought stress in plants. Observation of aflatoxin stimulation in 48-well plates in response to increasing inoculated wells sparked an investigation to determine if A. flavus volatiles influence aflatoxin production in neighboring colonies. Experiments controlling several culture conditions demonstrated a stimulation of aflatoxin production with increased well occupancy independent of pH buffer, moisture, or isolate. …

Social, Economic, And Political Events Affect Gender Equity In China, Nepal, And Nicaragua: A Matched, Interrupted Time-Series Study, Tuan T. Nguyen, Ashley Darnell, Amy Weissman, Edward A. Frongillo Jr., Roger Mathisen, Karin Lapping, Timothy D. Mastro, Mellissa Withers

Faculty Publications

: Progress in gender equity can improve health at the individual and country levels.: This study's objective was to analyze recent trends in gender equity and identify historical and contextual factors that contributed to changes in gender equity in three countries: China, Nepal, and Nicaragua.: To assess gender equity trends, we used the Gender Gap Index (GGI) from the World Economic Forum's Global Gender Gap Report (2006-2017). The GGI incorporated data on economic participation, educational attainment, health, and political empowerment for almost 150 countries. We selected China, Nepal, and Nicaragua because of their major changes in GGI and diversity in …