Digital Commons Network^™

Sources Of Variation In Nutrient Intakes Among Men In Shanghai, China, Hui Cai, Gong Yang, Yong-Bing Xiang, James R. Hébert, Da-Ke Liu, Wei Zheng, Xiao-Ou Shu

Faculty Publications

Background and objective: Random errors, from any source, will attenuate epidemiological risk estimates. Before we launched the Shanghai Men’s Health Study (SMHS), a large population-based cohort study investigating the diet–cancer association among Chinese men, a dietary calibration study was conducted among 96 men aged 40–75 years (mean age 56.5 years), with biweekly 24-hour dietary recalls (24HDRs) implemented over a 1-year period. Data from this study were analysed to evaluate the nature and magnitude of variances for intake of 26 nutrients among SMHS participants, to compare variance ratios of 26 nutrients among Chinese men and women and individuals in other studies, …

Search For Lepton-Flavor Violation At Hera, S. Chekanov, M. Derrick, S. Magill, S. Miglioranzi, B. Musgrave, J. Repond, R. Yoshida, Margarita C. K. Mattingly, N. Pavel, A. G. Yagües Molina, P. Antonioli, G. Bari, M. Basile, L. Bellagamba, D. Boscherini, A. Bruni, G. Bruni, G. Cara Romeo, L. Cifarelli, F. Cindolo, A. Contin, M. Corradi, S. De Pasquale, P. Giusti, G. Iacobucci, A. Margotti, A. Montanari, R. Nania, F. Palmonari, A. Pesci, A. Polini

Faculty Publications

A search for lepton-flavor-violating interactions ep → μX and ep → τX has been performed with the ZEUS detector using the entire HERA I data sample, corresponding to an integrated luminosity of 130 pb-1. The data were taken at center-of-mass energies, √s, of 300 and 318 GeV. No evidence of lepton-flavor violation was found, and constraints were derived on leptoquarks (LQs) that could mediate such interactions. For LQ masses below √s, limits were set on λeq1 √β ℓq, where λeq1 is the coupling of the LQ to an electron and a first-generation quark q1, and βℓq is the branching ratio …

Association Of The 17-Kda Extrinsic Protein With Photosystem Ii In Higher Plants, Glen D. Meades, Anne Mclachlan, Larry Sallans, Patrick A. Limbach, Laurie K. Frankel, Terry M. Bricker

Faculty Publications

The structural association of the spinach 17-kDa extrinsic protein of photosystem II with other extrinsic and membrane-bound components of the photosystem was investigated by labeling the 17-kDa extrinsic protein with the amino-group-specific reagent N-hydroxysuccinimidobiotin both on intact photosystem II membranes or as a free protein in solution. After isolation of the biotinylated molecules, the modified 17-kDa proteins were allowed to rebind to photosystem II membranes which were depleted of the 17-kDa component. Differential binding of the protein biotinylated in solution compared to unmodified 17-kDa protein or 17-kDa protein modified on PSII membranes was observed. This indicated possible steric or ionic …

Highly Efficient Fluorescence Detection In Picoliter Volume Liquid-Core Waveguides, Aaron R. Hawkins, John P. Barber, Dongliang Yin

Faculty Publications

We report loss improvement and fluorescence detection in integrated antiresonant reflecting optical waveguides with liquid cores. The minimum waveguide loss is reduced to 0.33/cm by compensating for thickness variations in the fabrication process. We demonstrate fluorescence detection from as few as 490 molecules in a 57 pl core using these optimized waveguides. We measure angular fluorescence collection factors as high as 15% per facet in good agreement with theory. This demonstrates the potential of integrated hollow-core waveguides as optical sensors for single-molecule spectroscopy.

Detection Of Homocysteine And Cysteine, Weihua Wang, Oleksandr Rusin, Xiangyang Xu, Kyu Kwang Kim, Jorge O. Escobedo, Sayo O. Fakayode, Kristin A. Fletcher, Mark Lowry, Corin M. Schowalter, Candace M. Lawrence, Frank R. Fronczek, Isiah M. Warner, Robert M. Strongin

Faculty Publications

At elevated levels, homocysteine (Hcy, 1) is a risk factor for cardiovascular diseases, Alzheimer's disease, neural tube defects, and osteoporosis. Both 1 and cysteine (Cys, 3) are linked to neurotoxicity. The biochemical mechanisms by which 1 and 3 are involved in disease states are relatively unclear. Herein, we describe simple methods for detecting either Hey or Cys in the visible spectral region with the highest selectivity reported to date without using biochemical techniques or preparative separations. Simple methods and readily available reagents allow for the detection of Cys and Hey in the range of their physiologically relevant levels. New HPLC …

Development And Validation Of The Theistic Spiritual Outcome Survey, P. Scott Richards, Timothy B. Smith, Marion Schowalter, Michael E. Berrett, Randy K. Hardman

Faculty Publications

We developed the Spiritual Outcome Scale (SOS) to measure the spiritual outcomes of psychotherapy from a theistic spiritual perspective. A 17-item version of the SOS was found to have adequate reliability and validity in a sample of college students. Three factors emerged from the analyses that corresponded to subscales labeled Love of God, Love of Others, and Love of Self. Correlations with measures of psychological outcomes were statistically significant. In subsequent analyses, the SOS was administered over an 8-week period to a sample of inpatient women with eating disorders and to two samples from inpatient psychological clinics in Germany. The …

Trehalose Loading Through The Mitochondrial Permeability Transition Pore Enhances Desiccation Tolerance In Rat Liver Mitochondria, Xiang Hong Liu, Alptekin Aksan, Michael A. Menze, Steven C. Hand, Mehmet Toner

Faculty Publications

Trehalose has extensively been used to improve the desiccation tolerance of mammalian cells. To test whether trehalose improves desiccation tolerance of mammalian mitochondria, we introduced trehalose into the matrix of isolated rat liver mitochondria by reversibly permeabilizing the inner membrane using the mitochondrial permeability transition pore (MPTP). Measurement of the trehalose concentration inside mitochondria using high performance liquid chromatography showed that the sugar permeated rapidly into the matrix upon opening the MPTP. The concentration of intra-matrix trehalose reached 0.29 mmol/mg protein (∼190 mM) in 5 min. Mitochondria, with and without trehalose loaded into the matrix, were desiccated in a buffer …

Multiple Bromodomain Genes Are Involved In Restricting The Spread Of Heterochromatic Silencing At The Saccharomyces Cerevisiae Hmr-Trna Boundary, Nithya Jambunathan, Adam W. Martinez, Elizabeth C. Robert, Nneamaka B. Agochukwu, Megan E. Ibos, Sandra L. Dugas, David Donze

Faculty Publications

The transfer RNA gene downstream from the HMR locus in S. cerevisiae functions as part of a boundary (barrier) element that restricts the spread of heterochromatic gene silencing into the downstream region of chromosome III. A genetic screen for identifying additional genes that, when mutated, allow inappropriate spreading of silencing from HMR through the tRNA gene was performed. YTA7, a gene containing bromodomain and ATPase homologies, was identified multiple times. Previously, others had shown that the bromodomain protein Bdf1p functions to restrict silencing at yeast euchromatin- heterochromatin boundaries; therefore we deleted nonessential bromodomain-containing genes to test their effects on heterochromatin …

Bioassay-Guided Fractionation Of Rubus Suavissimus Leaf Extracts Possessing Nf-Κb Inhibitory Activities And A Separable Cytotoxicity, Dong Liu, Zhanguo Gao, Jian Zhang, Jianping Ye, Zhijun Liu

Faculty Publications

Leaf extract of Rubus suavissimus S. Lee (Rosaceae), a perennial woody shrub indigenous to southern China, was tested for its biological activity in the regulation of transcription factor NF-κB (nuclear factor kappa B) A NF-κB luciferase reporter cell line was used in this study. The crude extract inhibited NF-κB at 100-1000 μg/ml concentrations with minor cytotoxicity. This finding prompted two more rounds of fractionation toward separating the active fraction from the cytotoxic fraction. Five fractions were obtained in the first round of fractionation, of which RUS-C20 was found to be the most potent in inhibiting NF-κB without signs of cytotoxicity …

Nursing Education And Service Collaboration: Making A Difference In The Clinical Learning Environment, Sheri P. Palmer, Amy Harmer Cox, Lynn Clark Callister, Vickie Johnsen, Geraldine Matsumura

Faculty Publications

This article focuses on innovative collaborative steps that were identified in recent research conducted by these authors on the relationship between academia and service. These steps are currently being implemented in the hope of improving the important role that the critical environment plays in student nurses' education. Few factors in nursing education are as important as the clinical environment in which students do their training. This article elaborates on these steps and offers practical suggestions for improving the relationship between academia and service.

Genomic Rearrangements By Line-1 Insertion-Mediated Deletion In The Human And Chimpanzee Lineages, Kyudong Han, Shurjo K. Sen, Jianxin Wang, Pauline A. Callinan, Jungnam Lee, Richard Cordaux, Ping Liang, Mark A. Batzer

Faculty Publications

Long INterspersed Elements (LINE-1s or L1s) are abundant non-LTR retrotransposons in mammalian genomes that are capable of insertional mutagenesis. They have been associated with target site deletions upon insertion in cell culture studies of retrotransposition. Here, we report 50 deletion events in the human and chimpanzee genomes directly linked to the insertion of L1 elements, resulting in the loss of ∼18 kb of sequence from the human genome and ∼15 kb from the chimpanzee genome. Our data suggest that during the primate radiation, L1 insertions may have deleted up to 7.5 Mb of target genomic sequences. While the results of …

Influences Of Dietary Soy Isoflavones On Metabolism But Not Nociception And Stress Hormone Responses In Ovariectomized Female Rats, Edwin D. Lephart, Lihong H. Bu, Kenneth Setchell

Faculty Publications

Isoflavones, the most abundant phytoestrogens in soy foods, are structurally similar to 17beta-estradiol. Few studies have examined the nociception and stress hormone responses after consumption of soy isoflavones. Methods: In this study, ovariectomized (OVX) female Long-Evans rats were fed either an isoflavone-rich diet (Phyto-600) or an isoflavone-free diet (Phyto-free). We examined the effects of soy isoflavones on metabolism by measuring body weights, food/water intake, adipose tissue weights as well as serum leptin levels. Also, circulating isoflavone levels were quantified. During chemically induced estrous, nociceptive thresholds were recorded. Then, the animals were subjected to a stressor and stress hormone levels were …

Immunocytochemical Localization Of Small-Conductance, Calcium-Dependent Potassium Channels In Astrocytes Of The Rat Supraoptic Nucleus, William E. Armstrong, Adam Rubrum, Ryoichi Teruyama, Chris T. Bond, John P. Adelman

Faculty Publications

Supraoptic nucleus (SON) neurons possess a prominent afterhyperpolarization (AHP) that contributes to spike patterning. This AHP is probably underlain by a small-conductance, CA2+-dependent, K+ type 3 (SK3) channel. To determine the distribution of SK3 channels within the SON, we used immunocytochemistry in rats and in transgenic mice with a regulatory cassette on the SK3 gene, allowing regulated expression with dietary doxycycline (DOX). In rats and wild-type mice, SK3 immunostaining revealed an intense lacy network surrounding SON neurons, with weak staining in neuronal somata and dendrites. In untreated, conditional SK3 knockout mice, SK3 was overexpressed, but the pericellular pattern in the …

Tracking Alu Evolution In New World Primates, David A. Ray, Mark A. Batzer

Faculty Publications

Background: Alu elements are Short INterspersed Elements (SINEs) in primate genomes that have proven useful as markers for studying genome evolution, population biology and phylogenetics. Most of these applications, however, have been limited to humans and their nearest relatives, chimpanzees. In an effort to expand our understanding of Alu sequence evolution and to increase the applicability of these markers to non-human primate biology, we have analyzed available Alu sequences for loci specific to platyrrhine (New World) primates. Results: Branching patterns along an Alu sequence phylogeny indicate three major classes of platyrrhine-specific Alu sequences. Sequence comparisons further reveal at least three …

Revelations From The Blackmun Papers On The Development Of Death Penalty Law , Martha Dragich

Faculty Publications

Professor Dragich uses the Blackmun papers to augment our understanding of Justice Blackmun's "evolution" on the question of capital punishment. Though the evolution was gradual, she finds that the case of Warren McCleskey seems to have deeply affected Justice Blackmun.

Justice Blackmun And The Spirit Of Liberty, Richard C. Reuben

Faculty Publications

As we see in this symposium, Justice Harry Blackmun is as controversial in death as he was in life. We live in a time of increasing absolutism, where things are either black or white, red or blue, you are either for me or against me, my way or the highway. It is when we are swayed by the sirens of absolutism that we are most likely to make mistakes, for absolutism diminishes our capacity to see nuance, much less to appreciate and account for it in our reasoning. This is a dangerous thing in a court, and in a democracy. …

Advancing Public Interest Practitioner Research Skills In Legal Education, Randy J. Diamond

Faculty Publications

The information revolution has dramatically altered the legal research landscape, expanding the bounds of legal authority. Practitioner research requires more than traditional legal research. It also encompasses factual investigation, non-legal information, interdisciplinary and audience research. Many new lawyers are ill-prepared to research novel and unusual situations, to cope with unwritten laws and local customs, and to meet shifting authority expectations.

A Preliminary Test Of A Student-Centered Intervention On Increasing Physical Activity In Underserved Adolescents, Dawn K. Wilson, Alexandra E. Evans, Joel Williams, Gary Mixon, John R. Sirard, Russell R. Pate

Faculty Publications

Background - Previous studies have shown that choice and self-initiated behavior changes are important for increasing intrinsic motivation and physical activity (PA), however, little of this research has focused on underserved adolescents.

Purpose - This study examined the effects of a 4-week student-centered intervention on increasing PA in underserved adolescents.

Methods - Twenty-eight students in the intervention school were matched (on race, percentage on free or reduced-price lunch program, gender, and age) with 20 students from another school who served as the comparison group (30 girls, 18 boys; ages 10-12 years; 83% African American; 83% on free or reduced-price lunch). …

Should Antitrust Education Be Mandatory (For Law School Administrators)?, Royce De R. Barondes, Thomas A. Lambert

Faculty Publications

The Executive Committee of the Association of American Law Schools has adopted a Statement of Good Practices that purports to limit the times when law schools may make offers to hire faculty members at other schools. Schools are generally not to make offers for indefinite appointments to professors on other faculties after March 1, subject to extension for two months with the consent of the incumbent's dean. They also are not to make offers contemplating resignation from a current faculty position more than two weeks following those deadlines. Proceeding on the assumption that the AALS policy, whose express terms are …

The Promise And Perils Of Collaborative Law, John M. Lande

Faculty Publications

Getting people to use an interest-based approach in negotiation has been a difficult problem. Experts provide helpful suggestions for changing the game, though these are usually limited to case-by-case efforts within a culture of adversarial negotiation. Collaborative law (CL) is an important innovation that establishes a general norm of interest-based negotiation and intentionally develops a new legal culture. This article describes CL's promise and potential perils, focusing particularly on the perils to complement the literature touting the promise.

The Relationship Between Leisure-Time Physical Activity And The Metabolic Syndrome: An Examination Of Nhanes Iii, 1988-1994, Katrina D. Dubose, Cheryl L. Addy, Barbara E. Ainsworth, Gregory A. Hand, J. Larry Durstine

Faculty Publications

No abstract provided.

Collaboration, Anita Coleman

Faculty Publications

This is a presentation (15) slides at the 2005 ASIS&T Annual Meeting, Charlotte, N.C. on October 31, session on Collaboration in Digital Libraries: Luminous Ideas from Health Informatics, Academic Libraries, and Historical Archives 

Book Ownership And Authorial Identity: Reconstructing The (Im)Personal Library Of Arthur Hugh Clough, Patrick G. Scott

Faculty Publications

Describes the different evidence that survives for the personal libraries owned by two Victorian poets, Alfred Tennyson and Arthur Hugh Clough, and discusses the ways in which such book-ownership is (and is not) usable as evidence about the author's thought and writing. This paper was originally presented at the North American Victorian Studies Association Conference, Charlottesville, VA, October 1, 2005.

Recruitment And Retention Of Emergency Medical Technicians: A Qualitative Study, P. Daniel Patterson, Janice C. Probst, Katherine H. Leith, Sara J. Corwin, M. Paige Powell

Faculty Publications

Emergency medical technicians (EMTs) are critical to out-of-hospital care, but maintaining staff can be difficult. The study objective was to identify factors that contribute to recruitment and retention of EMTs and paramedics. Information was drawn from three focus groups of EMT-Basic, EMT-Intermediate, and EMT-Paramedic personnel recruited from participants at an annual conference. Thoughts and feelings of EMTs and paramedics were investigated using eight questions designed to explore entry into emergency medical services, what it is like to be an EMT or paramedic, and the EMT educational process. Data were analyzed at the group level for common themes using NVivo. For …

Acoustic And Spectral Characteristics Of Young Children’S Fricative Productions: A Developmental Perspective, Shawn L. Nissen, Robert Allen Fox

Faculty Publications

Scientists have made great strides toward understanding the mechanisms of speech production and perception. However, the complex relationships between the acoustic structures of speech and the resulting psychological percepts have yet to be fully and adequately explained, especially in speech produced by younger children. Thus, this study examined the acoustic structure of voiceless fricatives /f, s, b/ produced by adults and typically developing children from 3 to 6 years of age in terms of multiple acoustic parameters durations, normalized amplitude, spectral slope, and spectral moments. It was found that the acoustic parameters of spectral slope and variance commonly excluded from …

Acoustic And Spectral Characteristics Of Young Children's Fricative Productions: A Developmental Perspective, Shawn L. Nissen, Robert Allen Fox

Faculty Publications

Scientists have made great strides toward understanding the mechanisms of speech production and perception. However, the complex relationships between the acoustic structures of speech and the resulting psychological percepts have yet to be fully and adequately explained, especially in speech produced by younger children. Thus, this study examined the acoustic structure of voiceless fricatives (/f, θ, s, ʃ/) produced by adults and typically developing children from 3 to 6 years of age in terms of multiple acoustic parameters (durations, normalized amplitude, spectral slope, and spectral moments). It was found the acoustic parameters of spectral slope and variance (commonly excluded from …

Moral Responsiveness And Discontinuity In Therapy: A Qualitative Study, Jason B. Whiting Phd, R. Scott Nebeker, Stephen T. Fife

Faculty Publications

Phenomenological qualitative methods were used to identify and describe moral elements in therapeutic relationships. Using the relational philosophy of E. Levinas (1961/1969, 1979/1987) as a base, data in which therapists and clients identified and described morally responsive experiences in therapy sessions were analyzed. These moments were often unexpected and included categories of surprise, interruption, willingness to change, and clarifications/repairs. Additional moral phenomena related to therapists' attitudes included asymmetrical indebtedness, attitude of serving, and tentativeness of diagnosis. Identified moments of moral responsiveness were frequently associated with clients' progress in therapy. This suggests that conceptually smooth and uninterrupted therapy may be less …

Emc’S Quiet Superman, Meredith Jones-Gray

Faculty Publications

No abstract provided.

Multijet Production In Neutral Current Deep Inelastic Scattering At Hera And Determination Of ΑS, S. Chekanov, M. Derrick, S. Magill, S. Miglioranzi, B. Musgrave, J. Repond, R. Yoshida, Margarita C. K. Mattingly, N. Pavel, A. G. Yagües Molina, P. Antonioli, G. Bari, M. Basile, L. Bellagamba, D. Boscherini, A. Bruni, G. Bruni, G. Cara Romeo, L. Cifarelli, F. Cindolo, A. Contin, M. Corradi, S. De Pasquale, P. Giusti, G. Iacobucci, A. Margotti, A. Montanari, R. Nania, F. Palmonari, A. Pesci, A. Polini

Faculty Publications

Multijet production rates in neutral current deep inelastic scattering have been measured in the range of exchanged boson virtualities 10 < Q 2 < 5000 GeV2. The data were taken at the ep collider HERA with centre-of-mass energy √s = 318 GeV using the ZEUS detector and correspond to an integrated luminosity of 82.2pb-1. Jets were identified in the Breit frame using the kT cluster algorithm in the longitudinally invariant inclusive mode. Measurements of differential dijet and trijet cross sections are presented as functions of jet transverse energy (ET,Bjet), pseudorapidity (ηLABjet) and Q2 with ET,Bjet > 5 GeV and -1 < ηLABjet < 2.5. Next-to-leading-order QCD calculations describe the data well. The value of the strong coupling constant αs(MZ), determined from the ratio of the trijet to dijet cross sections, is αs(MZ) = 0.1179 ± 0.0013 (stat.)-0.0046-0.0028 (exp.) -0.0046+0.0064 (th.). © Springer-Verlag / Società Italiana di Fisica 2005.

Hurricane-Forced Upwelling And Chlorophyll A Enhancement Within Cold-Core Cyclones In The Gulf Of Mexico, Nan D. Walker, Robert R. Leben, Shreekanth Balasubramanian

Faculty Publications

Clear skies, subsequent to Hurricane Ivan's passage across the Gulf of Mexico in September 2004, provided a unique opportunity to investigate upper ocean responses to a major hurricane. Oceanic cyclonic circulation was rapidly intensified by the hurricane's wind field (59-62 m s^-1), maximizing upwelling and surface cooling (3-7°C) in two large areas along Ivan's track. Upward isothermal displacements of 50-65 m, computed from wind stress and sea surface height changes, caused rapid ventilation of thermoclines and nutriclines, leading to phytoplankton blooms with peak concentrations 3-4 days later. Wind speed changes along Ivan's track demonstrated that the cool waters …