Digital Commons Network^™

The Congressional Failure To Enforce Equal Protection Through The Elementary And Secondary Education Act, Derek W. Black

Faculty Publications

No abstract provided.

Six Rna Viruses And Forty-One Hosts: Viral Small Rnas And Modulation Of Small Rna Repertoires In Vertebrate And Invertebrate Systems, Poornima Parameswaran, Ella Sklan, Courtney Wilkins, Trever Burgon, Melanie A. Samuel, Rui Lu, K. Mark Ansel, Vigo Heissmeyer, Shirit Einav, William Jackson, Tammy Doukas, Suman Paranjape, Charlotta Polacek, Flavia Barreto Dos Santos, Roxana Jalili, Farbod Babrzadeh, Baback Gharizadeh, Dirk Grimm, Mark Kay, Satoshi Koike, Peter Sarnow, Mostafa Ronaghi, Shou Wei Ding, Eva Harris, Marie Chow, Michael S. Diamond, Karla Kirkegaard, Jeffrey S. Glenn, Andrew Z. Fire

Faculty Publications

We have used multiplexed high-throughput sequencing to characterize changes in small RNA populations that occur during viral infection in animal cells. Small RNA-based mechanisms such as RNA interference (RNAi) have been shown in plant and invertebrate systems to play a key role in host responses to viral infection. Although homologs of the key RNAi effector pathways are present in mammalian cells, and can launch an RNAi-mediated degradation of experimentally targeted mRNAs, any role for such responses in mammalian host-virus interactions remains to be characterized. Six different viruses were examined in 41 experimentally susceptible and resistant host systems. We identified virus-derived …

Laboratory Evaluation Of Novaluron As A Rodent Feed-Through Insecticide Against Sand Fly Larvae (Diptera: Psychodidae), T. M. Mascari, L. D. Foil

Faculty Publications

Experiments were conducted to evaluate novaluron as a feed-through larvicide to control immature phlebotomine sand flies (Phlebotomus papatasi Scopoli) (Diptera: Psychodidae). The minimum effective concentration of novaluron against sand fly larvae fed hamster feces treated with novaluron, or feces of hamsters fed a diet containing novaluron, was 9.88 and 9.88 × 10-1 mg/kg, respectively. Feces of novaluron-treated hamsters were held under conditions simulating the inside of a rodent burrow for up to 30 d, and all larvae that consumed these feces died before pupation; a significant reduction in treated larval survival relative to control was observed when the feces were …

Effects Of Transgenic Rice Expressing Bacillus Thuringiensis Cry1ab Protein On Ground-Dwelling Collembolan Community In Postharvest Seasons, Y. Y. Bai, R. H. Yan, G. Y. Ye, F. N. Huang, J. A. Cheng

Faculty Publications

During 20052008, field studies were conducted at two locations in Chongqing, China, to assess the potential effects of transgenic rice expressing Bacillus thuringiensis (Bt) Cry1Ab protein on the nontarget ground-dwelling collembolan community in three postharvest seasons. Collembolans in non-Bt and Bt rice fields were sampled with pitfall traps during each of two postharvest seasons of 2005/2006 and 2006/2007 and litterbag traps during each of three postharvest seasons of 2005/2006, 2006/2007, and 2007/2008. Ground-dwelling collembolans in rice fields during the postharvest seasons were abundant, whereas community densities varied considerably between the two locations and among the three seasons. A total of …

Thirteen Ways Of Looking At Buck V. Bell: Thoughts Occasioned By Paul Lombardo's "Three Generations, No Imbeciles", Michelle Oberman

Faculty Publications

Paul Lombardo's recent book, Three Generations, No Imbeciles: Eugenics, the Supreme Court and Buck v. Bell, chronicles the history of state-sponsored sterilization over the course of the 20th century. As a historical endeavor, it is rich and rewarding, permitting the reader a broad understanding of the social, cultural and legal context for the case that inspired Oliver Wendell Holmes' famous quotation, "Three generations of imbeciles are enough." Lombardo's work masterfully ties the eugenics movement of the early 20th century to the broader policies informing the government's role in regulating reproduction .

Lombardo's book, which I originally picked up solely …

History Of Hope College: Forty Years Of Presidents And Growth Change, Geoffrey D. Reynolds

Faculty Publications

History of Hope College: Forty Years of Presidents and Growth Change is the fifth of five short articles about the history of Hope College, located in Holland, Michigan.

Greenhouse Gas Regulation Under The Clean Air Act: Structure, Effects, And Implications Of A Knowable Pathway, Nathan D. Richardson, Arthur G. Fraas, Dallas Butraw

Faculty Publications

It appears inevitable, absent legislative intervention, that regulation under the Clean Air Act (CAA) will move beyond mobile sources to the industrial and power facilities that emit most U.S. greenhouse gas (GHG) emissions. We analyze the mechanisms available to the EPA for regulating such sources, and identify one, New Source Performance Standards (NSPS) as the most predictable, likely, and practical, i.e. knowable, pathway. Based on the legal structure of the NSPS and the EPA’s traditional approach, we analyze a hypothetical GHG NSPS for one sector, coal electricity generation. This analysis indicates that efficiency improvements and perhaps biomass cofiring could be …

Dangers In Prescription Drugs: Filling A Private Law Gap In The Healthcare Debate, David G. Owen

Faculty Publications

No abstract provided.

A Lanthanide-Based Chemosensor For Bioavailable Fe3+ Using A Fluorescent Siderophore: An Assay Displacement Approach, Karen M. Orcutt, William Scott Jones, Andrea Mcdonald, David Schrock, Karl J. Wallace

Faculty Publications

The measurement of trace analytes in aqueous systems has become increasingly important for understanding ocean primary productivity. In oceanography, iron (Fe) is a key element in regulating ocean productivity, microplankton assemblages and has been identified as a causative element in the development of some harmful algal blooms. The chemosenor developed in this study is based on an indicator displacement approach that utilizes time-resolved fluorescence and fluorescence resonance energy transfer as the sensing mechanism to achieve detection of Fe3+ ions as low as 5 nM. This novel approach holds promise for the development of photoactive chemosensors for ocean deployment.

Effects Of Metal Compounds With Distinct Physicochemical Properties On Iron Homeostasis And Antibacterial Activity In The Lungs: Chromium And Vanadium, Mitchell D. Cohen, Maureen Sisco, Colleen Prophete, Kotaro Yoshida, Lung-Chi Chen, Judith T. Zelikoff, Jason Smee, Alvin A. Holder, Jacqueline Stormhuerner, Debbie C. Crans, Andrew J. Ghio

Faculty Publications

In situ reactions of metal ions or their compounds are important mechanisms by which particles alter lung immune responses. The authors hypothesized that major determinants of the immunomodulatory effect of any metal include its redox behavior/properties, oxidation state, and/or solubility, and that the toxicities arising from differences in physicochemical parameters are manifest, in part, via differential shifts in lung iron (Fe) homeostasis. To test the hypotheses, immunomodulatory potentials for both pentavalent vanadium (V(V); as soluble metavanadate or insoluble vanadium pentoxide) and hexavalent chromium (Cr(VI); as soluble sodium chromate or insoluble calcium chromate) were quantified in rats after inhalation (5 h/day …

Fiduciary Administration: Rethinking Popular Representation In Agency Rulemaking, Evan J. Criddle

Faculty Publications

Do administrative agencies undermine popular sovereignty when they make federal law? Over the last several decades, some scholars have argued that rulemaking by unelected agency officials imperils popular sovereignty and that federal law should resolve the apparent tension between regulatory practice and democratic principle by allowing the President to serve as a proxy for the "will of the people" in the administrative state. According to this view, placing federal rulemaking power firmly within the President's managerial control would advance popular preferences throughout the federal system.

This conventional wisdom is misguided. As political scientists have long recognized, the electorate's relative disengagement …

Helping Child Welfare Workers Improve Cultural Competence By Utilizing Spiritual Genograms With Native American Families And Children, Gordon E. Limb, David R. Hodge

Faculty Publications

This study represents an initial step at giving child welfare workers an important assessment tool as they seek to provide culturally and spiritually competent services to Native American families and children. In order to determine the relevancy and consistency of utilizing a modified spiritual genogram assessment tool with Native Americans, 50 Native American experts reviewed, rated, and gave feedback on its use. Results showed that while there were limitations in utilizing spiritual genograms, this assessment tool could help child welfare workers become more culturally competent as they develop interventions with Native American families and children.

Pastors’ Perspectives On Assimilating New Members: Part 1–Challenges And Needs, Jane Thayer

Faculty Publications

No abstract provided.

Virus Discovery By Deep Sequencing And Assembly Of Virus-Derived Small Silencing Rnas, Qingfa Wu, Yingjun Luo, Rui Lu, Nelson Lau, Eric C. Lai, Wan Xiang Li, Shou Wei Ding

Faculty Publications

In response to infection, invertebrates process replicating viral RNA genomes into siRNAs of discrete sizes to guide virus clearance by RNA interference. Here, we show that viral siRNAs sequenced from fruit fly, mosquito, and nematode cells were all overlapping in sequence, suggesting a possibility of using siRNAs for viral genome assembly and virus discovery. To test this idea, we examined contigs assembled from published small RNA libraries and discovered five previously undescribed viruses from cultured Drosophila cells and adult mosquitoes, including three with a positive-strand RNA genome and two with a dsRNA genome. Notably, four of the identified viruses exhibited …

Intergovernmental Fiscal Relations In The Czech Republic: A Survey Of Municipality Expenditure Patterns, Phillip J. Bryson, Scott M. Smith, Gary C. Cornia

Faculty Publications

A survey of Czech local officials probes perceptions of intergovernmental relations. Perceived autonomy and municipality size are tested as autonomy indicators. The impact of the city size on municipal expenditures from different funding sources is evaluated. Cross-tabulations of city size identify revenue sources for various expenditures and activities. For all expenditures, use of funds is more effectively explained by city size than by the fiscal choices of high- and low-autonomy groups. A graphic analysis of relationships between expenditures and revenue sources complements the statistical analysis. Radar charts reveal sources for small versus large and low- versus high-autonomy cities.

Construction, Figures Of Merit And Testing Of A Single-Cell Fluorescence Excitation Spectroscopy System, Laura S. Hill, Tammi L. Richardson, Louisa T.M. Profeta, Timothy J. Shaw, Christopher J. Hintz, Benjamin S. Twining, Evelyn Lawrenz, Micheal L. Myrick

Faculty Publications

Characterization of phytoplankton community composition is critical to understanding the ecology and biogeochemistry of the oceans. One approach to taxonomic characterization takes advantage of differing pigmentation between algal taxa and thus differences in fluorescence excitation spectra. Analyses of bulk water samples, however, may be confounded by interference from chromophoric dissolved organic matter or suspended particulate matter. Here, we describe an instrument that uses a laser trap based on a Nikon TE2000-U microscope to position individual phytoplankton cells for confocal fluorescence excitation spectroscopy, thus avoiding interference from the surrounding medium. Quantitative measurements of optical power give data in the form of …

Structural Basis For The Photoconversion Of A Phytochrome To The Activated Pfr Form, Andrew T. Ulijasz, Gabriel Cornilescu, Claudia C. Cornilescu, Junrui Zhang, Mario Rivera, John L. Markley, Richard D. Vierstra

Faculty Publications

Phytochromes are a collection of bilin-containing photoreceptors that regulate numerous photoresponses in plants and microorganisms through their ability to photointerconvert between a red-light-absorbing, ground state (Pr) and a far-red-light-absorbing, photoactivated state (Pfr). Although the structures of several phytochromes as Pr have been determined, little is known about the structure of Pfr and how it initiates signalling. Here we describe the three-dimensional solution structure of the bilin-binding domain as Pfr, using the cyanobacterial phytochrome from Synechococcus OSB′. Contrary to predictions, light-induced rotation of the A pyrrole ring but not the Dring is the primary motion of the chromophore during photoconversion. Subsequent …

Psifr: An Integrated Resource For Prediction Of Protein Structure And Function, Shashi B. Pandit, Michal Brylinski, Hongyi Zhou, Mu Gao, Adrian K. Arakaki, Jeffrey Skolnick

Faculty Publications

In the post-genomic era, the annotation of protein function facilitates the understanding of various biological processes. To extend the range of function annotation methods to the twilight zone of sequence identity, we have developed approaches that exploit both protein tertiary structure and/or protein sequence evolutionary relationships. To serve the scientific community, we have integrated the structure prediction tools, TASSER, TASSER-Lite and METATASSER, and the functional inference tools, FINDSITE, a structure-based algorithm for binding site prediction, Gene Ontology molecular function inference and ligand screening, EFICAz2, a sequence-based approach to enzyme function inference and DBD-hunter, an algorithm for predicting DNA-binding proteins and …

Functional Genomics Tool: Gene Silencing In Ixodes Scapularis Eggs And Nymphs By Electroporated Dsrna, Shahid Karim, Emily Troiano, Thomas N. Mather

Faculty Publications

Background

Ticks are blood-sucking arthropods responsible for transmitting a wide variety of disease-causing agents, and constitute important public health threats globally. Ixodes scapularis is the primary vector of the Lyme disease agent in the eastern and central U.S. RNAi is a mechanism by which gene-specific double-stranded RNA (dsRNA) triggers degradation of homologous mRNA transcripts. Here, we describe an optimized protocol for effectively suppressing gene expression in the egg and nymphal stages of I. scapularis by electroporation.

Results

The genes encoding the putative Phospholipase A₂ (PLA₂), cytoplasmic Cystatin, Syntaxin-5, β-Actin and Calreticulin were targeted by delivering the dsRNA …

Bilitrienones From The Chemical Oxidation Of Dodecasubstituted Porphyrins, Owendi Ongayi, M. Graça H. Vicente, Brahma Ghosh, Frank R. Fronczek, Kevin M. Smith

Faculty Publications

The structure of the ring-opened product from direct oxidation of meso-tetra-arylporphyrins has been controversial for three decades. Herein we show that bilitrienones 2 are obtained from oxidation of metal-free dodecasubstituted porphyrins 1 in the presence of sodium nitrite, trifluoroacetic acid, and air oxygen. The presence of the para-nonyl groups in 1b stabilized the corresponding bilitrienone 2b, which was characterized by X-ray crystallography. In the absence of the para-nonyl groups bilitrienone 2a undergoes a rapid hydration reaction, giving biladienone 3a as the major isolated product. The molecular structures of 2b and 3a, and the photochemical isomerization of 3a are discussed. © …

Forgiveness As New Creation: Christ And The Moral Life Revisited, Lois E. Malcolm

Faculty Publications

No abstract provided.

Experiencing The Spirit: The Magnificat, Luther, And Feminists, Lois E. Malcolm

Faculty Publications

No abstract provided.

Pakistan’S Nuclear Weapons Program And Implications For Us National Security., Michael Tkacik

Faculty Publications

This article analyzes Pakistan’s nuclear weapons program and the characteristics of the environment in which the program is nested. These characteristics include Pakistan’s history of internal and external instability; nuclear saber rattling during crises; support for Islamic terrorism in order to advance state goals; indigenous production of many elements of its nuclear forces; possession of delivery and command and control systems with destabilizing characteristics; and finally, nuclear doctrine that appears to advocate first use of nuclear weapons. The article argues that the characteristics of Pakistan’s nuclear weapons program generate threats to US national security interests. The article examines six interrelated …

Administrative Decentralization: Should Districts And Regions Elect Their Own Leaders In Ghana?, Kwame Badu Antwi-Boasiako

Faculty Publications

Decentralization, leadership accountability, and empowerment of local leaders in Ghana are the main focus of this analysis. The paper discusses the complexities of decentralization and leadership responsibilities to effect changes toward a better path to socioeconomic development. It examines the problem with the Ghana Constitution as it pertains to local government elections and advocates for a constitutional amendment to empower districts and regions to elect their own leaders so that local governments in each district can be efficient and accountable to the electorate.

Using Video Feedback To Measure Self-Efficacy, Linda Bobo, Amanda Andrews

Faculty Publications

When a student has a high sense of self-efficacy, foreseeing success and providing positive guides and supports for performing the skill will usually occur. A low self-efficacy tends to predict failure and anticipation of what could go wrong. Videotape feedback provided to students has reported favorable outcomes. Self-efficacy could alter performance in learning a psychomotor competency skill (PCS). The purpose of this study was a) to assess the self-efficacy of athletic training students in learning to perform a PCS; and b) to measure the impact on self-efficacy by implementing an educational intervention of video feedback in learning to perform a …

Song Of Wekiva: Florida’S Wild River And Its Democratic Vista, Steve Phelan

Faculty Publications

Today hundreds of thousands enjoy the river every year. We are a different breed. When I take our children or my students to the river or walk along its sandy uplands to show them undiscovered and unmapped springs popping out of the ground, they and I are still abiding in a domesticated world that erects a substantial, almost impervious barrier to the wilderness of the river. Nevertheless, that wildness is built unconsciously into our bodies. We are not like the Timucuans, the first humans to come down the St. Johns River and eventually up the Wekiva to the two main …

Mirren's Autobiography: The Life And Poetry Of Marion Bernstein (1846-1906), Edward Cohen, Linda Fleming

Faculty Publications

No abstract provided.

International Human Rights Law In Japan: The View At Thirty, Timothy Webster

Faculty Publications

Japanese courts have become increasingly open to the use of international human rights law in the past two decades. This paper examines several of the key decisions that reflect the judiciary's embrace of international law, particularly in the areas of criminal procedure and minority rights. I argue that the judiciary has eclipsed the other branches of government as the primary disseminator of human rights norms in Japan.

Corporate Social Responsibility And Earnings Reporting, Mary F. Calegari, T. Chotigeat, M. A. Harjoto

Faculty Publications

Despite increasing interests on corporate social responsibility (CSR) activities among managers, the relationship between CSR and firm value through earnings reporting quality is still unclear. Absence of a strong positive effect of CSR on firm value has led researchers to believe that CSR is a res ult of a principal-agent issue between shareholders and managers. This study argues CSR represents a corporate culture that influences how a corporation reports its earnings. CSR influ ences earnings reporting ·instead ofearnings reporting drives CSR to delude shareholders. CSR induces better earnings reporting quality, therefore, CSR has an indirect but positive effect on firm …

Asian American Elders In The Twenty-First Century—Key Indicators Of Well-Being (Review), Sadhna Diwan

Faculty Publications

No abstract provided.