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Time-Courses In The Retention Of Food Material In The Bivalves Potamocorbula Amurensis And Macoma Balthica: Significance To The Absorption Of Carbon And Chromium, Alan W. Decho, Samuel N. Luoma Dec 1991

Time-Courses In The Retention Of Food Material In The Bivalves Potamocorbula Amurensis And Macoma Balthica: Significance To The Absorption Of Carbon And Chromium, Alan W. Decho, Samuel N. Luoma

Faculty Publications

Time courses for ingestion, retention and release via feces of microbial food was investigated using 2 bivalves with different feeding strategies, Potamocorbula amurensis, and Macoma balthica. The results showed 2 pathways for the uptake of food material in these clams. The first is represented by an initial label pulse in the feces. The second pathway operates over longer time periods. Inert 51Cr-labeled beads were used to determine time frames for these pathways. The first pathway, involving extracellular digestion and intestinal uptake, is relatively inefficient in the digestion of bacterial cells by P. amurensis but more efficient in …

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Dynamics Of Bacterioplankton Abundance And Production In Seagrass Communities Of A Hypersaline Lagoon, Gerardo Chin-Leo, Ronald Benner Jul 1991

Dynamics Of Bacterioplankton Abundance And Production In Seagrass Communities Of A Hypersaline Lagoon, Gerardo Chin-Leo, Ronald Benner

Faculty Publications

The significance of bacterioplankton in the flow of carbon and energy and in trophic dynamics of the upper Laguna Madre, Texas (USA), was estimated by measuring bacterioplankton abundance and production over an 18 mo period and over several diel cycles. Bacterioplankton production was estimated from incorporation rates of thymidine (DNA synthesis) and leucine (protein synthesis). These independent inhces of bacterial growth were generally in agreement and yielded nearly identical annual estimates of bacterial production (25.24 g C m-2 yr-1 based on thymidine and 25.12 g C m-2 yr-1based on leucine). Assuming a 30 % growth …

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Decrease In Skin Collagen Glycation With Improved Glycemic Control In Patients With Insulin-Dependent Diabetes Mellitus, Timothy J. Lyons, Karen E. Bailie, Daniel G. Dyer, John A. Dunn, John W. Baynes Jun 1991

Decrease In Skin Collagen Glycation With Improved Glycemic Control In Patients With Insulin-Dependent Diabetes Mellitus, Timothy J. Lyons, Karen E. Bailie, Daniel G. Dyer, John A. Dunn, John W. Baynes

Faculty Publications

Glycation, oxidation, and nonenzymatic browning of protein have all been implicated in the development of diabetic complications. The initial product of glycation of protein, fructoselysine (FL), undergoes further reactions, yielding a complex mixture of browning products, including the fluorescent lysine-arginine cross-link, pentosidine. Alternatively, FL may be cleaved oxidatively to form N(epsilon)-(carboxymethyl)lysine (CML), while glycated hydroxylysine, an amino-acid unique to collagen, may yield N(epsilon)-(carboxymethyl)hydroxylysine (CMhL). We have measured FL, pentosidine, fluorescence (excitation = 328 nm, emission = 378 nm), CML, and CMhL in insoluble skin collagen from 14 insulin-dependent diabetic patients before and after a 4-mo period of intensive therapy to …

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Osmophores, Floral Features, And Systematics Of Stanhopea (Orchidaceae), Kenneth J. Curry, Lorraine M. Mcdowell, Walter S. Judd, William Louis Stern May 1991

Osmophores, Floral Features, And Systematics Of Stanhopea (Orchidaceae), Kenneth J. Curry, Lorraine M. Mcdowell, Walter S. Judd, William Louis Stern

Faculty Publications

The floral fragrance glands (osmophores) of 18 species of Stanhopea and Sievekingia were examined through a series of developmental studies at light and electron microscope levels including late bud stages through postanthesis. Various characters were identified to be of potential systematic value and were recorded for each species. These characters included: texture of the osmophore surface, number of distinct cell layers comprising the osmophore, nature of lipid inclusions in osmophore cells, and presence or absence of plastoglobuli in osmophore amyloplasts. These characters were combined with traditional features of floral lip morphology for cladistic analysis. Sievekingia was the postulated outgroup. Stanhopea …

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The Devil's Disciple And The Learned Profession: Ambrose Bierce And The Practice Of Law In Gilded Age America, J. Gordon Hylton Jan 1991

The Devil's Disciple And The Learned Profession: Ambrose Bierce And The Practice Of Law In Gilded Age America, J. Gordon Hylton

Faculty Publications

No abstract provided.

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On Stopping And Restarting The Race: A Response To Malloy’S Law And Economics, David Ray Papke Jan 1991

On Stopping And Restarting The Race: A Response To Malloy’S Law And Economics, David Ray Papke

Faculty Publications

No abstract provided.

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Detection And Strain Differentiation Of Chlamydia Psittaci Mediated By A Two-Step Polymerase Chain Reaction, B. Kaltenboeck, K. G. Kousoulas, J. Storz Jan 1991

Detection And Strain Differentiation Of Chlamydia Psittaci Mediated By A Two-Step Polymerase Chain Reaction, B. Kaltenboeck, K. G. Kousoulas, J. Storz

Faculty Publications

Specific and sensitive amplification of major outer membrane protein (MOMP) gene DNA sequences of Chlamydia psittaci was achieved in a two-step polymerase chain reaction. First, oligonucleotide primers specific for 5' and 3' nontranslated regulatory regions of the MOMP gene were used in a polymerase chain reaction to amplify a DNA fragment of approximately 1,400 bp. A portion of this DNA fragment was amplified in a second reaction using a degenerate oligonucleotide primer specific for a DNA sequence contained within the 1,400-bp DNA fragment and one of the first-step primers. This method detected 10 cognate chlamydial genomes. C. psittaci MOMP genes …

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Comparison Of The Nucleotide And Deduced Amino Acid Sequences Of The S Genes Specified By Virulent And Avirulent Strains Of Bovine Coronaviruses, Xuming Zhang, Konstantin G. Kousoulas, Johannes Storz Jan 1991

Comparison Of The Nucleotide And Deduced Amino Acid Sequences Of The S Genes Specified By Virulent And Avirulent Strains Of Bovine Coronaviruses, Xuming Zhang, Konstantin G. Kousoulas, Johannes Storz

Faculty Publications

The entire nucleotide sequences of the spike glycoprotein (S) genes of the highly virulent bovine coronavirus (BCV) strain BCV-LY138, the avirulent BCV-L9 and related Norden Vaccine (BCV-Vaccine) strains were determined using the polymerase chain reaction (PCR) to amplify cDNAs obtained by reverse transcription of viral RNA, and to produce single strand cDNAs for DNA sequencing. The S gene sequences of these viral strains were compared with those of recently published strains BCV-Mebus, BCV-Quebec, and BCV-F15. An open reading frame of 4092 nucleotides, encoding a protein of 1363 amino acid residues, was found in all six strains. Frameshifts and insertions or …

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Comparison Of Bovine Coronavirus (Bcv) Antigens: Monoclonal Antibodies To The Spike Glycoprotein Distinguish Between Vaccine And Wild-Type Strains, Khalid A. Hussain, Johannes Storz, Konstantin G. Kousoulas Jan 1991

Comparison Of Bovine Coronavirus (Bcv) Antigens: Monoclonal Antibodies To The Spike Glycoprotein Distinguish Between Vaccine And Wild-Type Strains, Khalid A. Hussain, Johannes Storz, Konstantin G. Kousoulas

Faculty Publications

Monoclonal antibodies (MAbs) against two major structural proteins of the cell-adapted Mebus strain of bovine coronavirus (BCV-L9) were produced and characterized. Seven MAbs reacted with the peplomeric glycoprotein, gp100/S, while three MAbs reacted with the nucleoprotein p53/N in Western blot analysis of BCV polypeptides. MAbs to gp100/S reacted with discontinuous epitopes of gp100/S in Westerns under mild but not under standard denaturing conditions. In contrast, MAbs to p53/N reacted in both types of Westerns, and those epitopes were thus continuous. MAbs to p53/N failed to neutralize BCV infectivity, while 4 MAbs to gp100/S neutralized BCV effectively. Cross reactivity of MAbs …

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Hormone Secretion By Preimplantation Embryos In A Dynamic In Vitro Culture System, J. M. Wilson, D. D. Zalesky, C. R. Looney, K. R. Bondioli, R. R. Magness Jan 1991

Hormone Secretion By Preimplantation Embryos In A Dynamic In Vitro Culture System, J. M. Wilson, D. D. Zalesky, C. R. Looney, K. R. Bondioli, R. R. Magness

Faculty Publications

No abstract provided.

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Computer-Simulated Evaluation Of Possible Mechanisms For Quenching Heavy Metal Ion Activity In Plant Vacuoles: I. Cadmium, Jian Wang, Bill P. Evangelou, Mark T. Nielsen, George J. Wagner Jan 1991

Computer-Simulated Evaluation Of Possible Mechanisms For Quenching Heavy Metal Ion Activity In Plant Vacuoles: I. Cadmium, Jian Wang, Bill P. Evangelou, Mark T. Nielsen, George J. Wagner

Faculty Publications

Various mechanisms have been suggested for the quenching of Cd ion activity in plant vacuoles. These include solution complexation with organic acids and sulfhydryl-containing peptides and precipitation as sulfides. Because direct experimental support for these mechanisms is lacking and difficult to obtain, we have used a computer model to evaluate the quenching role of possible organic and inorganic ligands of tobacco cultured cells exposed to Cd. Results of this thermodynamic evaluation, which assumes that a chemical equilibrium state is met in the vacuole, support the conclusion that sulfhydryl-containing peptides and certain organic acids may form soluble Cd complexes. Although complexation …

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Evaluating Acrosome Interference Contrast Reaction Steps With Brightfield And Differential Microscopy Techniques, H. C. Steinholt, J. E. Chandler, V. Tirado Jan 1991

Evaluating Acrosome Interference Contrast Reaction Steps With Brightfield And Differential Microscopy Techniques, H. C. Steinholt, J. E. Chandler, V. Tirado

Faculty Publications

Acrosomal integrity of bovine spermatozoa was evaluated using naphthol yellow S-erythrosine B stain with bright field microscopy, .2% glutaraldehyde fixation with Nomarski optics, and live wet smears with Nomarski optics to compare the evaluation techniques. Four ejaculates per two Holstein bulls were enriched through a Percoll gradient, capacitated with heparin, and acrosome-reacted with lysophosphatidylcholine to prepare spermatozoa for in vitro fertilization. Spermatozoa samples were taken after each preparatory step, and percentage of intact acrosomes was evaluated with the stain, .2% glutaraldehyde, and live wet smear. Acrosomal integrity decreased with each preparatory step across all techniques. The decrease in integrity from …

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Accutane: Has Drug Regulation In The United States Reached Its Limits?, Joan H. Krause Jan 1991

Accutane: Has Drug Regulation In The United States Reached Its Limits?, Joan H. Krause

Faculty Publications

No abstract provided.

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The Reservation As Place: A South Dakota Essay, Frank Pommersheim Jan 1991

The Reservation As Place: A South Dakota Essay, Frank Pommersheim

Faculty Publications

No abstract provided.

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Effects Of Polyamine Biosynthesis Inhibitors On S-Adenosylmethionine Synthetase And S-Adenosylmethionine Decarboxylase Activities In Carrot Cell Cultures, Subhash C. Minocha, Rakesh Minocha, Atsushi Komamine Jan 1991

Effects Of Polyamine Biosynthesis Inhibitors On S-Adenosylmethionine Synthetase And S-Adenosylmethionine Decarboxylase Activities In Carrot Cell Cultures, Subhash C. Minocha, Rakesh Minocha, Atsushi Komamine

Faculty Publications

Changes in the activites of S-adcnosylmethionine (SAM) synthetase (methionine adenosyltransferase, EC 2.5.1.6.) and SAM decarboxylase (EC 4.1.1.50) were studied in carrot (Daucus carota) cell cultures in response to 2,4-dichlorophenoxyacetic acid (2,4-D) and several inhibitors of polyamine biosynthesis. Activity of SAM synthetase increased significantly during the first 5 days of culture under all conditions, maximum activity being observed in thc presence of 2,4-D. DL-α-Difluoromethylarginine (DFMA), I,L-α-difluoroinethylornithine (DFMO) and cyclohexylammonium sulfatc (CHAS) had little effect on the activity of this enzyme. The enzyme activity was lower than the controls in the presence of methylglyoxal bis(guany1hydrazone) (MGBG). The activity of SAM dccarboxylasc, which …

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In Defense Of Smith And Free Exercise Revisionism, William P. Marshall Jan 1991

In Defense Of Smith And Free Exercise Revisionism, William P. Marshall

Faculty Publications

No abstract provided.

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Freedom Of Speech And The Flag Anti-Desecration Amendment: Antinomies Of Constitutional Choice, William W. Van Alstyne Jan 1991

Freedom Of Speech And The Flag Anti-Desecration Amendment: Antinomies Of Constitutional Choice, William W. Van Alstyne

Faculty Publications

No abstract provided.

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Affirmative Action In The Marketplace Of Ideas, Rodney A. Smolla Jan 1991

Affirmative Action In The Marketplace Of Ideas, Rodney A. Smolla

Faculty Publications

No abstract provided.

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A Primer For Accompanying Secondary Student Groups To France. [Revised]., Jim Becker Jan 1991

A Primer For Accompanying Secondary Student Groups To France. [Revised]., Jim Becker

Faculty Publications

Many of the factors that can help ensure a successful and positive learning experience for teachers; leaders; chaperons; and students who travel and/or study in France are addressed in this publication. Although it is geared toward France; many of the ideas; especially the section on pre-departure orientation; can be applied to any travel group. The primary objectives of the booklet are to help teachers develop confidence in taking students abroad; to promote the "know before you go" mentality; and to provide an information resource. Among the nearly 100 topics covered are the following: itinerary; airport check-in; menus; post office; insurance; …

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Purification And Characterization Of A Dna Polymerase From The Cyanobacterium Anacystis Nidulans R2, Hsiu-Jin Lin, Gordon C. Cannon, Sabine Heinhorst Nov 1990

Purification And Characterization Of A Dna Polymerase From The Cyanobacterium Anacystis Nidulans R2, Hsiu-Jin Lin, Gordon C. Cannon, Sabine Heinhorst

Faculty Publications

A DNA polymerase has been highly purified from Anacystis nidulans R2. Electrophoretic analysis in sodium dodecyl sulfate-polyacrylamide gels revealed that the final fraction contains three bands of Mr 107,000, 93,000, and 51,000, respectively. Analysis of purified DNA polymerase activity in situ indicates that of the three polypeptides the Mr 107,000 species has the catalytic activities. The native molecular weight of the enzyme was estimated by glycerol gradient sedimentation to be 100,000. The enzyme has an absolute requirement for a divalent cation. Mg2+ can be replaced with Mn2+, but the DNA polymerase is less active. Potassium …

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Exercise Tolerance And Alcohol Intake: Blood Pressure Relation, G. Harley Hartung, Harold W. Kohl Iii, Steven N. Blair, Steven J. Lawrence, Ronald B. Harrist Nov 1990

Exercise Tolerance And Alcohol Intake: Blood Pressure Relation, G. Harley Hartung, Harold W. Kohl Iii, Steven N. Blair, Steven J. Lawrence, Ronald B. Harrist

Faculty Publications

The relations of systolic and diastolic blood pressures to alcohol intake and exercise tolerance levels in 15,612 men and 3,855 women were investigated. Alcohol intake was assessed by questionnaire and stratified into seven levels for men and six for women according to the ounces of ethanol consumed per week. Exercise tolerance was determined by maximal treadmill exercise testing and was categorized into six age-specific by sex-specific levels. Both systolic and diastolic blood pressure were significantly related to both alcohol intake and exercise tolerance levels in both men and women. These relations, which were positive for alcohol and negative for exercise …

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Contingency Contracting In The Low-Intensity Conflict Environment, Steve Bell Oct 1990

Contingency Contracting In The Low-Intensity Conflict Environment, Steve Bell

Faculty Publications

The United States military presence in Central and South America is under attack, both literally and politically, as bombings and sniper fire directed against U.S. military personnel characterize the low-intensity conflict (LIC) environment in certain parts of Latin America. The process of contracting support for U.S. forces in a LIC environment is oriented toward sustaining operations in the field, as opposed to contracting for mobilization or surge production. A significant characteristic of this field support is that a contracting officer (KO) deploys overseas with the supported military unit. Some of the unique concerns faced by a KO deploying with an …

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Suppression Of Lymphocyte Proliferation By A > 30,000 Molecular Weight Factor In Horse Conceptus-Conditioned Medium, T. L. Roth, K. L. White, D. L. Thompson, B. E. Barry, J. S. Capehart, D. R. Colborn, M. H. Rabb Aug 1990

Suppression Of Lymphocyte Proliferation By A > 30,000 Molecular Weight Factor In Horse Conceptus-Conditioned Medium, T. L. Roth, K. L. White, D. L. Thompson, B. E. Barry, J. S. Capehart, D. R. Colborn, M. H. Rabb

Faculty Publications

In this experiment we have identified and partially characterized the immunosuppressive activity of preimplantation horse conceptus-conditioned medium (HCCM). Horse conceptuses were nonsurgically flushed from mares at Days 9-10 (n = 6), 15-16 (n = 3), and 25-26 (n = 3). After incubating the conceptuses for 24 h in RPMI-1640 supplemented with 15% fetal calf serum (FCS) and 1% penicillin/streptomycin, HCCM was obtained from cultures and tested for immunosuppressive activity in lymphocyte proliferation assays. Peripheral blood lymphocytes obtained from randomly selected mares were stimulated with mitogens (pokeweed mitogen [PWM], concanavalin A [Con A], and phytohemagglutinin [PHA]) in cultures supplemented with 0%, …

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Academic Freedom, Hate Speech, And The Idea Of A University, Rodney A. Smolla Jul 1990

Academic Freedom, Hate Speech, And The Idea Of A University, Rodney A. Smolla

Faculty Publications

No abstract provided.

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Federal And State Water Quality Regulation And Law In Missouri, Peter N. Davis Apr 1990

Federal And State Water Quality Regulation And Law In Missouri, Peter N. Davis

Faculty Publications

This article discusses that law in two parts. The first part examines the federal and Missouri waste discharge regulatory system. The second part analyzes common law rights and remedies related to water pollution.

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Chloroplast And Mitochondrial: Dna Polymerases From Cultured Soybean Cells, Sabine Heinhorst, Gordon C. Cannon, Arthur Weissbach Apr 1990

Chloroplast And Mitochondrial: Dna Polymerases From Cultured Soybean Cells, Sabine Heinhorst, Gordon C. Cannon, Arthur Weissbach

Faculty Publications

DNA polymerases were purified from chloroplasts and mitochondria of cultured Glycine max cells. The chloroplast enzyme exists in two forms which are indistinguishable from each other biochemically. All three organellar enzymes have an estimated molecular weight of 85,000 to 90,000 and prefer poly(rA)dT12-18 over activated DNA as a template in vitro. Maximum activity of the chloroplast and mitochondrial DNA polymerases requires KCI and a reducing agent, and the enzymes are completely resistant to inhibitors of DNA polymerase a. Taken together, these properties classify the soybean organellar enzymes as DNA polymerases y. A unique feature that distinguishes the plant enzymes from …

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Public Organizational Existence: A Critique Of Individualism In Democratic Administration, Charles R. Davis Mar 1990

Public Organizational Existence: A Critique Of Individualism In Democratic Administration, Charles R. Davis

Faculty Publications

No abstract provided.

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Landscape Development And Coastal Wetland Losses In The Northern Gulf Of Mexico, R. Eugene Turner Feb 1990

Landscape Development And Coastal Wetland Losses In The Northern Gulf Of Mexico, R. Eugene Turner

Faculty Publications

The causes of the extensive (0.86%/yr; 288,414 ha/yr) and well documented dramatic and accelerating rate of coastal wetland loss in the northern Gulf of Mexico were investigated by an interdisciplinary university research team to discern the role of outer continental shelf development. The landscape changes and potential causal agents are emphasized herein. Natural driving factors include sea level rise and geological com- paction, which appear to remain constant this century, and sediment supply from the Mississippi River which has declined by 50%,since the 1950s. Man-made influences include hydrologic changes from river diversions, flood protection levees, an extensive canal and spoil …

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American Influence In Greece, 1917-1929, By Louis P. Cassimatis, Gerasimos Augustinos Feb 1990

American Influence In Greece, 1917-1929, By Louis P. Cassimatis, Gerasimos Augustinos

Faculty Publications

A review of American Influence in Greece, 1917-1929, by Louis P. Cassimatis

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Antimicrobial Susceptibilities Of Staphylococcal Species Isolated From Mammary Glands Of Unbred And Primigravid Dairy Heifers, P. Trinidad, S. C. Nickerson, D. G. Luther Jan 1990

Antimicrobial Susceptibilities Of Staphylococcal Species Isolated From Mammary Glands Of Unbred And Primigravid Dairy Heifers, P. Trinidad, S. C. Nickerson, D. G. Luther

Faculty Publications

Staphylococcal isolates from teat canal keratin and mammary secretion samples of unbred and primigravid Jersey heifers were tested in vitro for susceptibility to 12 antimicrobial agents. More than 92% of the 311 isolates were susceptible to all antimicrobial agents tested. Staphylococci other than Staphylococcus aureus demonstrated an overall susceptibility of 98.3% to all antibiotics, and Staphylococcus aureus demonstrated a 97% susceptibility. Across all staphylococcal species, susceptibility of isolates from secretion samples was 98.1%, and susceptibility of isolates from teat canal keratin samples was 93.1%. Differences in susceptibilities were observed among herds. © 1990, American Dairy Science Association. All rights reserved.

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