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Identification And Characterization Of Putative Translocated Effector Proteins Of The Edwardsiella Ictaluri Type Iii Secretion System, Lidiya P. Dubytska, Matthew L. Rogge, Ronald L. Thune May 2016

Identification And Characterization Of Putative Translocated Effector Proteins Of The Edwardsiella Ictaluri Type Iii Secretion System, Lidiya P. Dubytska, Matthew L. Rogge, Ronald L. Thune

Faculty Publications

© 2016 Dubytska et al. Edwardsiella ictaluri, a major pathogen in channel catfish aquaculture, encodes a type III secretion system (T3SS) that is essential for intracellular replication and virulence. Previous work identified three putative T3SS effectors in E. ictaluri, and in silico analysis of the E. ictaluri genome identified six additional putative effectors, all located on the chromosome outside the T3SS pathogenicity island. To establish active translocation by the T3SS, we constructed translational fusions of each effector to the amino-terminal adenylate cyclase (AC) domain of the Bordetella pertussis adenylate cyclase toxin CyaA. When translocated through the membrane of the Edwardsiella-containing …

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Fibroblast Growth Factor-23 (Fgf-23) Levels Differ Across Populations By Degree Of Industrialization, Shennin N. Yuen, Kramer Holly, Amy Luke, Pascal Bovet, Jacob Plange-Rhule, Terrence Forrester, Vicki Lambert, Myles Wolf, Pauline Camacho, Regina Harders, Lara Dugas, Richard Cooper, Ramon Durazo-Arvizu May 2016

Fibroblast Growth Factor-23 (Fgf-23) Levels Differ Across Populations By Degree Of Industrialization, Shennin N. Yuen, Kramer Holly, Amy Luke, Pascal Bovet, Jacob Plange-Rhule, Terrence Forrester, Vicki Lambert, Myles Wolf, Pauline Camacho, Regina Harders, Lara Dugas, Richard Cooper, Ramon Durazo-Arvizu

Faculty Publications

Context: Compensatory increases in fibroblast growth factor 23 (FGF23) with increasing phosphate intake may adversely impact health. However, population and clinical studies examining the link between phosphate intake and FGF23 levels have focused mainly on populations living in highly industrialized societies in which phosphate exposure may be homogenous. Objective: The objective of the study was to contrast dietary phosphate intake, urinary measures of phosphate excretion, and FGF23 levels across populations that differ by the level of industrialization. Design: This was a cross-sectional analysis of three populations. Setting: The study was conducted in Maywood, Illinois; Mahé Island, Seychelles; and Kumasi, Ghana. …

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Discovery Of A New Repeat Family In The Callithrix Jacchus Genome, Miriam Konkel, Mark Batzer, Brygg Ullmer, Erika L. Arceneaux, Sreeja Sanampudi, Sarah A. Brantley, Robert Hubley, Arian F.A. Smit May 2016

Discovery Of A New Repeat Family In The Callithrix Jacchus Genome, Miriam Konkel, Mark Batzer, Brygg Ullmer, Erika L. Arceneaux, Sreeja Sanampudi, Sarah A. Brantley, Robert Hubley, Arian F.A. Smit

Faculty Publications

We identified a novel repeat family, termed Platy-1, in the Callithrix jacchus (common marmoset) genome that arose around the time of the divergence of platyrrhines and catarrhines and established itself as a repeat family in New World monkeys (NWMs). A full-length Platy-1 element is ∼100 bp in length, making it the shortest known short interspersed element (SINE) in primates, and harbors features characteristic of non-LTR retrotransposons. We identified 2268 full-length Platy-1 elements across 62 subfamilies in the common marmoset genome. Our subfamily reconstruction and phylogenetic analyses support Platy-1 propagation throughout the evolution of NWMs in the lineage leading to C. …

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New Compartment Model Analysis Of Lean-Mass And Fat-Mass Growth With Overfeeding, Dmytro Shumilov, Steven B. Heymsfield, Leanne M. Redman, Kesava Kalluri, Joyoni Dey May 2016

New Compartment Model Analysis Of Lean-Mass And Fat-Mass Growth With Overfeeding, Dmytro Shumilov, Steven B. Heymsfield, Leanne M. Redman, Kesava Kalluri, Joyoni Dey

Faculty Publications

Objectives: Mathematical models of lean- and fat-mass growth with diet are useful to help describe and potentially predict the fat- and lean-mass change with different diets as a function of consumed protein and fat calories. Most of the existing models do not explicitly account for interdependence of fat-mass on the lean-mass and vice versa. The aim of this study was to develop a new compartmental model to describe the growth of lean and fat mass depending on the input of dietary protein and fat, and accounting for the interdependence of adipose tissue and muscle growth. Methods: The model was fitted …

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Mitochondrial Dna Variation In Southeastern Pre-Columbian Canids, Kristin E. Brzeski, Melissa B. Debiasse, David R. Rabon, Michael J. Chamberlain, Sabrina S. Taylor May 2016

Mitochondrial Dna Variation In Southeastern Pre-Columbian Canids, Kristin E. Brzeski, Melissa B. Debiasse, David R. Rabon, Michael J. Chamberlain, Sabrina S. Taylor

Faculty Publications

The taxonomic status of the red Wolf (Canis rufus) is heavily debated, but could be clarified by examining historic specimens from the southeastern United States. We analyzed mitochondrial DNA (mtDNA) from 3 ancient (350-1900 year olds) putative Wolf samples excavated from middens and sinkholes within the historic red Wolf range. We detected 3 unique mtDNA haplotypes, which grouped with the coyote mtDNA clade, suggesting that the canids inhabiting southeastern North America prior to human colonization from Europe were either coyotes, which would vastly expand historic coyote distributions, an ancient coyote-Wolf hybrid, or a North American evolved red Wolf lineage related …

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The Use Of Marine Aquaculture Solid Waste For Nursery Production Of The Salt Marsh Plants Spartina Alterniflora And Juncus Roemerianus, Heather M. Joesting, Reginald B. Blaylock, Patrick Biber, Andrew Ray May 2016

The Use Of Marine Aquaculture Solid Waste For Nursery Production Of The Salt Marsh Plants Spartina Alterniflora And Juncus Roemerianus, Heather M. Joesting, Reginald B. Blaylock, Patrick Biber, Andrew Ray

Faculty Publications

Recent technological advances in marine shrimp and finfish aquaculture alleviate many of the environmental risks associated with traditional aquaculture, but challenges remain in cost-effective waste management. Liquid effluent from freshwater aquaculture systems has been shown to be effective in agricultural crop production (i.e., aquaponics), but few studies have explored the potential for reuse of marine aquaculture effluent, particularly the solid fraction. The purpose of this study was to investigate the use of marine aquaculture solid waste as a nutrient source for the nursery production of two salt tolerant plants commonly used in coastal salt marsh restoration, Spartina alterniflora (smooth cordgrass) …

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Chesapeake Aquaculture, Garth Woodruff May 2016

Chesapeake Aquaculture, Garth Woodruff

Faculty Publications

No abstract provided.

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Ubisol-Q10 Prevents Glutamate-Induced Cell Death By Blocking Mitochondrial Fragmentation And Permeability Transition Pore Opening, Santosh Kumari, Suresh L. Mehta, Gaolin Z. Milledge, Xinyu Huang, P Andy Li Apr 2016

Ubisol-Q10 Prevents Glutamate-Induced Cell Death By Blocking Mitochondrial Fragmentation And Permeability Transition Pore Opening, Santosh Kumari, Suresh L. Mehta, Gaolin Z. Milledge, Xinyu Huang, P Andy Li

Faculty Publications

Mitochondrial dysfunction and oxidative stress are the major events that lead to the formation of mitochondrial permeability transition pore (mPTP) during glutamate-induced cytotoxicity and cell death. Coenzyme Q10 (CoQ10) has widely been used for the treatment of mitochondrial disorders and neurodegenerative diseases. Comparing to traditional lipid-soluble CoQ10, water soluble CoQ10 (Ubisol-Q10) has high intracellular and intra-mitochondrial distribution. The aims of the present study are to determine the neuroprotective effects of Ubisol-Q10 on glutamate-induced cell death and to explore its functional mechanisms. HT22 neuronal cells were exposed to glutamate. Cell viability was measured and mitochondrial fragmentation was assessed by mitochondrial imaging. …

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Post-Feminism For Children: Feminism ‘Repackaged’ In The Bratz Films, Sarah Anna Becker, Danielle Thomas, Michael R. Cope Apr 2016

Post-Feminism For Children: Feminism ‘Repackaged’ In The Bratz Films, Sarah Anna Becker, Danielle Thomas, Michael R. Cope

Faculty Publications

After their release in 2001, Bratz dolls carved into Barbie’s previously monopolistic share of teen doll sales. Amidst their growing popularity, cultural critics expressed a host of concerns about Bratz dolls, especially over how they sexualize youth, but the line grew to include a host of products like costumes, makeup kits, games, books, clothing, and movies. It also inspired new, similar doll lines from other toy companies. In this article, we situate the Bratz’s popularity in a specific cultural moment tied to the history of modern feminism. We use a content analysis of the Bratz movie series to explore the …

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Alternative Promoters Of Peg3 With Maternal Specificity, Bambarendage P.U. Perera, Joomyeong Kim Apr 2016

Alternative Promoters Of Peg3 With Maternal Specificity, Bambarendage P.U. Perera, Joomyeong Kim

Faculty Publications

Peg3 (paternally expressed gene 3) is an imprinted gene localized within an evolutionarily conserved 500-kb domain in human chromosome 19q13.4 and mouse proximal chromosome 7. In the current study, we have identified three alternative promoters for mouse Peg3 and one alternative promoter for human PEG3. These alternative promoters are localized within the 200-kb upstream region of human and mouse PEG3, which is well conserved and thus predicted to harbor several cis-regulatory elements for the PEG3 domain. In the mouse, two of these alternative promoters drive maternal-specific expression of Peg3 specifically in the hypothalamus of the adult brain, while the remaining …

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Eating Increases Oxidative Damage In A Reptile, Michael W. Butler, Thomas J. Lutz, Haralambos Fokidis, Zachary R. Stahlschmidt Apr 2016

Eating Increases Oxidative Damage In A Reptile, Michael W. Butler, Thomas J. Lutz, Haralambos Fokidis, Zachary R. Stahlschmidt

Faculty Publications

While eating has substantial benefits in terms of both nutrient and energy acquisition, there are physiological costs associated with digesting and metabolizing a meal. Frequently, these costs have been documented in the context of energy expenditure while other physiological costs have been relatively unexplored. Here, we tested whether the seemingly innocuous act of eating affects either systemic pro-oxidant (reactive oxygen metabolite, ROM) levels or antioxidant capacity of corn snakes (Pantherophis guttatus) by collecting plasma during absorptive (peak increase in metabolic rate due to digestion of a meal) and non-absorptive (baseline) states. When individuals were digesting a meal, there was a …

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The Ecologist's Field Guide To Sequence-Based Identification Of Biodiversity, Simon Creer, Kristy Deiner, Serita D. Frey, Dorota Porazinska, Pierre Taberlet, W. Kelley Thomas, Caitlin Potter, Holly M. Bik Apr 2016

The Ecologist's Field Guide To Sequence-Based Identification Of Biodiversity, Simon Creer, Kristy Deiner, Serita D. Frey, Dorota Porazinska, Pierre Taberlet, W. Kelley Thomas, Caitlin Potter, Holly M. Bik

Faculty Publications

The past 100 years of ecological research has seen substantial progress in understanding the natural world and likely effects of change, whether natural or anthropogenic. Traditional ecological approaches underpin such advances, but would additionally benefit from recent developments in the sequence-based quantification of biodiversity from the fields of molecular ecology and genomics. By building on a long and rich history of molecular taxonomy and taking advantage of the new generation of DNA sequencing technologies, we are gaining previously impossible insights into alpha and beta diversity from all domains of life, irrespective of body size. While a number of complementary reviews …

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Controlling Humans And Machines, Bryant Walker Smith Apr 2016

Controlling Humans And Machines, Bryant Walker Smith

Faculty Publications

No abstract provided.

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The Horne Dilemma: Protecting Property's Richness And Frontiers, Lynda L. Butler Apr 2016

The Horne Dilemma: Protecting Property's Richness And Frontiers, Lynda L. Butler

Faculty Publications

In a 2015 decision, the Supreme Court concluded that real and personal property should not be treated differently under the Takings Clause and that a government condition requiring raisin growers, in certain years, to reserve a percentage of their crop for the government to manage in noncompetitive venues was a per se physical taking. The decision to treat both real and personal property as equally worthy of protection under the Takings Clause has merit given the weak historical evidence suggesting stronger protection for land and the importance of personal property to income generation and capital development in a modern society. …

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Helminthr: An R Interface To The London Natural History Museum's Host-Parasite Database, Tad Dallas Apr 2016

Helminthr: An R Interface To The London Natural History Museum's Host-Parasite Database, Tad Dallas

Faculty Publications

© 2016 Nordic Society Oikos. The understanding of the diversity and distribution of helminth parasites is currently constrained by the limited number of host-parasite interaction databases, and the difficulty in accessing existing data. The London Natural History Museum's Host-Parasite Database represents one such underutilized database, containing over a quarter million helminth parasite occurrence records, accessible through a web interface. To enable users to programmatically search and manipulate data from this database, I developed an R package called helminthR. Here, I introduce the core functions of the package, and detail how helminthR can be used to obtain host-parasite interaction records, citations …

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Genome Content And Phylogenomics Reveal Both Ancestral And Lateral Evolutionary Pathways In Plant-Pathogenic Streptomyces Species, Jose C. Huguet-Tapia, Tristan Lefebure, Jonathan H. Badger, Dongli Guan, Gregg S. Pettis, Michael J. Stanhope, Rosemary Loria Apr 2016

Genome Content And Phylogenomics Reveal Both Ancestral And Lateral Evolutionary Pathways In Plant-Pathogenic Streptomyces Species, Jose C. Huguet-Tapia, Tristan Lefebure, Jonathan H. Badger, Dongli Guan, Gregg S. Pettis, Michael J. Stanhope, Rosemary Loria

Faculty Publications

© 2016, American Society for Microbiology. All Rights Reserved. Streptomyces spp. are highly differentiated actinomycetes with large, linear chromosomes that encode an arsenal of biologically active molecules and catabolic enzymes. Members of this genus are well equipped for life in nutrient-limited environments and are common soil saprophytes. Out of the hundreds of species in the genus Streptomyces, a small group has evolved the ability to infect plants. The recent availability of Streptomyces genome sequences, including four genomes of pathogenic species, provided an opportunity to characterize the gene content specific to these pathogens and to study phylogenetic relationships among them. Genome …

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Laser Ablation Sample Transfer For Localized Lc-Ms/Ms Proteomic Analysis Of Tissue, Fabrizio Donnarumma, Kermit K. Murray Apr 2016

Laser Ablation Sample Transfer For Localized Lc-Ms/Ms Proteomic Analysis Of Tissue, Fabrizio Donnarumma, Kermit K. Murray

Faculty Publications

© 2016 John Wiley & Sons, Ltd. We have developed a mid-infrared laser ablation sampling technique for nano-flow liquid chromatography coupled with tandem mass spectrometry proteomic profiling of discrete regions from biological samples. Laser ablation performed in transmission geometry was used to transfer material from 50-μm thick tissue sections mounted on a glass microscope slide to a capturing solvent. Captured samples were processed using filter-aided sample preparation and enzymatically digested to produce tryptic peptides for data-dependent analysis with an ion trap mass spectrometer. Comparison with ultraviolet laser capture microdissection from neighboring regions on the same tissue section revealed that infrared …

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Growth Dynamics Of A Laminated Microbial Mat In Response To Variable Irradiance In An Antarctic Lake, Ian Hawes, Anne D. Jungblut, Maciej K. Obryk, Peter T. Doran Apr 2016

Growth Dynamics Of A Laminated Microbial Mat In Response To Variable Irradiance In An Antarctic Lake, Ian Hawes, Anne D. Jungblut, Maciej K. Obryk, Peter T. Doran

Faculty Publications

© 2016 John Wiley & Sons Ltd. Laminated microbial mats are important ecosystem components of perennially ice-covered Antarctic dry valley lakes. In order to understand better their response to changing environment, we made observations and carried out a manipulation experiment to determine their response to variations in irradiance in Lake Hoare (77°38′ S, 162°53′ E). Ice transparency was the most variable parameter that affected benthic light dose, both spatially and between years. Patterns of lamina accrual corresponded to irradiance history, with laminae that were initiated in high transmission years thicker than those from low transmission years. A shading experiment confirmed …

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14th Congress Of Combustion By-Products And Their Health Effects—Origin, Fate, And Health Effects Of Combustion-Related Air Pollutants In The Coming Era Of Bio-Based Energy Sources, Eva Weidemann, Patrik L. Andersson, Terry Bidleman, Christoffer Boman, Danielle J. Carlin, Elena Collina, Stephania A. Cormier, Sandra C. Gouveia-Figueira, Brian K. Gullett, Christer Johansson, Donald Lucas, Lisa Lundin, Staffan Lundstedt, Stellan Marklund, Malin L. Nording, Nuria Ortuño, Asmaa A. Sallam, Florian M. Schmidt, Stina Jansson Apr 2016

14th Congress Of Combustion By-Products And Their Health Effects—Origin, Fate, And Health Effects Of Combustion-Related Air Pollutants In The Coming Era Of Bio-Based Energy Sources, Eva Weidemann, Patrik L. Andersson, Terry Bidleman, Christoffer Boman, Danielle J. Carlin, Elena Collina, Stephania A. Cormier, Sandra C. Gouveia-Figueira, Brian K. Gullett, Christer Johansson, Donald Lucas, Lisa Lundin, Staffan Lundstedt, Stellan Marklund, Malin L. Nording, Nuria Ortuño, Asmaa A. Sallam, Florian M. Schmidt, Stina Jansson

Faculty Publications

© 2016, Springer-Verlag Berlin Heidelberg. The 14th International Congress on Combustion By-Products and Their Health Effects was held in Umeå, Sweden from June 14th to 17th, 2015. The Congress, mainly sponsored by the National Institute of Environmental Health Sciences Superfund Research Program and the Swedish Research Council for Environment, Agricultural Sciences and Spatial Planning, focused on the “Origin, fate and health effects of combustion-related air pollutants in the coming era of bio-based energy sources”. The international delegates included academic and government researchers, engineers, scientists, policymakers and representatives of industrial partners. The Congress provided a unique forum for the discussion of …

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Phylogenetic And Epigenetic Footprinting Of The Putative Enhancers Of The Peg3 Domain, Joomyeong Kim, An Ye Apr 2016

Phylogenetic And Epigenetic Footprinting Of The Putative Enhancers Of The Peg3 Domain, Joomyeong Kim, An Ye

Faculty Publications

© 2016 Kim, Ye. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. The Peg3 (Paternally Expressed Gene 3) imprinted domain is predicted to be regulated through a large number of evolutionarily conserved regions (ECRs) that are localized within its middle 200-kb region. In the current study, we characterized these potential cis-regulatory regions using phylogenetic and epigenetic approaches. According to the results, the majority of these ECRs are potential enhancers for the transcription of the …

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Revisiting Coptotermes (Isoptera: Rhinotermitidae): A Global Taxonomic Road Map For Species Validity And Distribution Of An Economically Important Subterranean Termite Genus, Thomas Chouvenc, Hou Feng Li, James Austin, Christian Bordereau, Thomas Bourguignon, Stephen L. Cameron, Eliana M. Cancello, Reginaldo Constantino, Ana Maria Costa-Leonardo, Paul Eggleton, Theodore A. Evans, Brian Forschler, J. Kenneth Grace, Claudia Husseneder, Jan Křeček, Chow Yang Lee, Timothy Lee, Nathan Lo, Matthew Messenger, Aaron Mullins, Alain Robert, Yves Roisin, Rudolf H. Scheffrahn, David Sillam-Dussès, Jan Šobotník, Allen Szalanski, Yoko Takematsu, Edward L. Vargo, Akinori Yamada, Tsuyoshi Yoshimura, Nan Yao Su Apr 2016

Revisiting Coptotermes (Isoptera: Rhinotermitidae): A Global Taxonomic Road Map For Species Validity And Distribution Of An Economically Important Subterranean Termite Genus, Thomas Chouvenc, Hou Feng Li, James Austin, Christian Bordereau, Thomas Bourguignon, Stephen L. Cameron, Eliana M. Cancello, Reginaldo Constantino, Ana Maria Costa-Leonardo, Paul Eggleton, Theodore A. Evans, Brian Forschler, J. Kenneth Grace, Claudia Husseneder, Jan Křeček, Chow Yang Lee, Timothy Lee, Nathan Lo, Matthew Messenger, Aaron Mullins, Alain Robert, Yves Roisin, Rudolf H. Scheffrahn, David Sillam-Dussès, Jan Šobotník, Allen Szalanski, Yoko Takematsu, Edward L. Vargo, Akinori Yamada, Tsuyoshi Yoshimura, Nan Yao Su

Faculty Publications

No abstract provided.

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Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch Apr 2016

Determination Of Sperm Concentration Using Flow Cytometry With Simultaneous Analysis Of Sperm Plasma Membrane Integrity In Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Terrence R. Tiersch

Faculty Publications

Control of sperm concentration is required to ensure consistent and reproducible results for cryopreservation and in vitro fertilization protocols. Determination of sperm concentration is traditionally performed with a counting chamber (e.g., hemocytometer), or more recently with a spectrophotometer. For small-sized biomedical model fishes, the availability of sperm sample is limited to microliters, so it is desirable to develop fast and accurate approaches for concentration determination that also minimize sample use. In this study, a new approach was developed for sperm concentration determination using a flow cytometer (Accuri C6, BD Biosciences, San Jose, CA) with simultaneous measurement of sperm membrane integrity …

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A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch Apr 2016

A Procedure-Spanning Analysis Of Plasma Membrane Integrity For Assessment Of Cell Viability In Sperm Cryopreservation Of Zebrafish Danio Rerio, Huiping Yang, Jonathan Daly, Carrie Carmichael, Jen Matthews, Zoltan M. Varga, Terrence Tiersch

Faculty Publications

The goal of this study was to evaluate plasma membrane integrity and motility for zebrafish sperm quality assessment along the cryopreservation pathway - from sample collection through refrigerated storage, cryoprotectant equilibration, freezing, thawing, and fertilization. The objectives were to: (1) evaluate the effects of osmolality, extender, and refrigerated storage on sperm plasma membrane integrity and motility, and (2) compare cryopreservation of sperm from farm-raised and well-characterized research populations by evaluating motility and membrane integrity of fresh, post-equilibration (before freezing) and post-thaw sperm, and post-thaw fertility. Osmolality, extender, and storage time each influenced sperm motility and membrane integrity. Isotonic osmolality showed …

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Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield Apr 2016

Climate, Habitat Associations And The Potential Distributions Of Neotropical Birds: Implications For Diversification Across The Andes, Carlos Daniel Cadena, Carlos A. Pedraza, Robb T. Brumfield

Faculty Publications

Contrary to the long-held view that the uplift of the Andes spurred biotic diversification by causing vicariance across multiple lineages with formerly continuous distributions, recent work suggests that dispersal across the Andes occurring after major uplift episodes was a major driver of speciation in Neotropical birds, with the ability of lineages to persist and disperse in the landscape being strong predictors of speciation. However, where and when dispersal events across the Andes occurred is unknown, and the role of climatic fluctuations and associated shifts in vegetation in promoting cross-Andes dispersal at different moments remains to be studied. We used models …

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Suprachiasmatic Nucleus And Subparaventricular Zone Lesions Disrupt Circadian Rhythmicity But Not Light-Induced Masking Behavior In Nile Grass Rats, Andrew J. Gall, Dorela D. Shuboni, Lily Yan, Antonio A. Nunez, Laura Smale Apr 2016

Suprachiasmatic Nucleus And Subparaventricular Zone Lesions Disrupt Circadian Rhythmicity But Not Light-Induced Masking Behavior In Nile Grass Rats, Andrew J. Gall, Dorela D. Shuboni, Lily Yan, Antonio A. Nunez, Laura Smale

Faculty Publications

The ventral subparaventricular zone (vSPVZ) receives direct retinal input and influences the daily patterning of activity in rodents, making it a likely candidate for the mediation of acute behavioral responses to light (i.e., masking). We performed chemical lesions aimed at the vSPVZ of diurnal grass rats (Arvicanthis niloticus) using N-methyl-D,L-aspartic acid (NMA), a glutamate agonist. Following NMA lesions, we placed grass rats in various lighting conditions (e.g., 12:12 light-dark, constant dark, constant light); presented a series of light pulses at circadian times (CT) 6, 14, 18, and 22; and placed them in a 7-h ultradian cycle to assess behavioral masking. …

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The Disembodied Rule And The Rule Made Flesh: Propositions, Illustrations, And The Placement Of Citations, Stephen E. Smith Apr 2016

The Disembodied Rule And The Rule Made Flesh: Propositions, Illustrations, And The Placement Of Citations, Stephen E. Smith

Faculty Publications

No abstract provided.

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The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez Apr 2016

The Potential Role Of Polyamines In Gill Epithelial Remodeling During Extreme Hypoosmotic Challenges In The Gulf Killifish, Fundulus Grandis, Ying Guan, Guo Xia Zhang, Shujun Zhang, Beau Domangue, Fernando Galvez

Faculty Publications

© 2016 Elsevier Inc. Polyamines are a family of low molecular weight organic cations produced in part by the coordinated actions of arginase II (Arg II) and ornithine decarboxylase (Odc). Although gill polyamine homeostasis is affected by acute transfer to fresh water, little is known of its function in fish osmoregulation. The current study investigated the role of polyamines in the compensatory response of hypoosmotic challenge in the euryhaline fish, Fundulus grandis. Adult F. grandis were acclimated to 5ppt water, transferred abruptly to 5, 2, 1, 0.5 and 0.1 ppt water, and assessed for osmoregulatory function, gill morphology, and polyamine …

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Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss Apr 2016

Environmental Stress In The Gulf Of Mexico And Its Potential Impact On Public Health, B. Singleton, J. Turner, L. Walter, N. Lathan, D. Thorpe, P. Ogbevoen, J. Daye, D. Alcorn, S. Wilson, J. Semien, T. Richard, T. Johnson, K. Mccabe, J. J. Estrada, F. Galvez, C. Velasco, K. Reiss

Faculty Publications

© 2015 Elsevier Inc. The Deepwater Horizon (DWH) oil spill in the Gulf of Mexico was the largest maritime oil spill in history resulting in the accumulation of genotoxic substances in the air, soil, and water. This has potential far-reaching health impacts on cleanup field workers and on the populations living in the contaminated coastal areas. We have employed portable airborne particulate matter samplers (SKC Biosampler Impinger) and a genetically engineered bacterial reporter system (umu-ChromoTest from EBPI) to determine levels of genotoxicity of air samples collected from highly contaminated areas of coastal Louisiana including Grand Isle, Port Fourchon, and Elmer's …

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Breast Cancer Risk Perceptions Among Relatives Of Women With Uninformative Negative Brca1/2 Test Results: The Moderating Effect Of The Amount Of Shared Information, Deborah O. Himes Msn, Aprn-Bc, Margaret F. Clayton, Gary W. Donaldson, Lee Ellington, Saundra S. Buys, Anita Y. Kinney Apr 2016

Breast Cancer Risk Perceptions Among Relatives Of Women With Uninformative Negative Brca1/2 Test Results: The Moderating Effect Of The Amount Of Shared Information, Deborah O. Himes Msn, Aprn-Bc, Margaret F. Clayton, Gary W. Donaldson, Lee Ellington, Saundra S. Buys, Anita Y. Kinney

Faculty Publications

The most common result of BRCA1/2 mutation testing when performed in a family without a previously identified mutation is an uninformative negative test result. Women in these families may have an increased risk for breast cancer because of mutations in non-BRCA breast cancer predisposition genes, including moderate- or low-risk genes, or shared environmental factors. Genetic counselors often encourage counselees to share information with family members, however it is unclear how much information counselees share and the impact that shared information may have on accuracy of risk perception in family members. We evaluated 85 sisters and daughters of women who received …

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Molecular Markers Located On The Dgat1, Cast, And Lepr Genes And Their Associations With Milk Production And Fertility Traits In Holstein Cattle, R. Hill, A. Canal, K. Bondioli, R. Morell, M. D. Garcia Mar 2016

Molecular Markers Located On The Dgat1, Cast, And Lepr Genes And Their Associations With Milk Production And Fertility Traits In Holstein Cattle, R. Hill, A. Canal, K. Bondioli, R. Morell, M. D. Garcia

Faculty Publications

© FUNPEC-RP. The objective of the present study was to investigate single nucleotide polymorphisms (SNPs) located in three candidate genes previously reported to have effects on fertility and milk production traits in a population of 123 Holstein cows. The milk production traits evaluated included lifetime averages of milk yield, protein concentration, and fat concentration. Fertility traits evaluated included lifetime averages of services per conception and days-open. Candidate genes included those encoding diacylglycerol acyltransferase (DGAT1), leptin receptor (LEPR), and calpastatin (CAST). A total of 60 SNPs were selected (20 per gene) at equidistant locations on each candidate gene to identify potential …

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