Digital Commons Network^™

Adolescent Sexual And Reproductive Health In The Gambia., Sandhya Lohani

Electronic Theses and Dissertations

Adolescence is a crucial period in human development. Knowledge and awareness about when to have children, when to have sex, and use of contraceptives impacts the chances of teenage pregnancy and risk of sexually transmitted diseases. The objective of this study is to determine the prevalence and associated risk factors related to age of sexual debut, teenage pregnancy, and use of contraceptives in the Gambia. This study also compares the data with 2014 survey research conducted in the Gambia. The total study population were 50 adolescents aged 13-21 years from Bafuluto and surrounding communities in the Gambia. Among 50 respondents, …

Computational Prediction, Characterization, And Methodology Development For Two-Dimensional Nanostructures: Phosphorene And Phosphide Binary Compounds., Congyan Zhang

Electronic Theses and Dissertations

In this thesis, a comprehensive computational simulation was carried out for predicting, characterizing, and applications of two-dimensional (2D) materials. The newly discovered GaP and InP layers were selected as an example to demonstrate how to explore new 2D materials using computational simulations. The performance of phosphorene as the anode material of Lithium-ion battery was discussed as the example of the application of 2D material. Furthermore, the semi-empirical Hamiltonian for phosphorous and lithium elements have been developed for our future work on the application of phosphorus and lithium-based systems. The novel 2D materials of GaP and InP binary compounds were found …

Student Engagement In Post-Soviet Higher Education: Coping With Academic Quality Problems., J. L. Mettille

Electronic Theses and Dissertations

This dissertation investigates the experiences of administrators, instructional faculty, and students within a post-Soviet higher education institution in the Kyrgyz Republic using qualitative field research and case study methods. This study identifies the perceived potential of Western benchmarks of effective educational practice to evince positive change in the institution as well as their propriety and relevance in this context. In addition, the propositions underlying the student engagement construct and overarching the benchmarks are evaluated based upon participant responses and classroom observation data. Twenty higher education practitioners and twenty students participated in semi-structured interviews preceding and following seven classroom observations. The …

Exploration Of Radiation Damage Mechanism In Mems Devices., Pranoy Deb Shuvra

Electronic Theses and Dissertations

We explored UV, X-ray and proton radiation damage mechanisms in MEMS resonators. T-shaped MEMS resonators of different dimensions were used to investigate the effect of radiation. Radiation damage is observed in the form of resistance and resonance frequency shift of the device. The resistance change indicates a change in free carrier concentration and mobility, while the resonance frequency change indicates a change in mass and/or elastic constant. For 255nm UV radiation, we observed a persistent photoconductivity that lasts for about 60 hours after radiation is turned off. The resonance frequency also decreases 40-90 ppm during irradiation and slowly recovers at …

The Role Of Aldehyde Dehygrogenase 2 In Liver Injury Caused By Vinyl Chloride And High-Fat Diet., Liya Chen

Electronic Theses and Dissertations

Background. Vinyl chloride (VC) is an abundant environmental contaminant that causes steatohepatitis at high exposure levels. We have shown previously that low concentrations of VC exacerbate high fat diet (HFD)-induced liver injury in mice. The mechanisms involved in the progression of liver injury caused by VC and HFD include oxidative stress, inflammation, metabolic and mitochondrial dysfunction. Mitochondrial aldehyde dehydrogenase 2 (ALDH2) serves as a key line of defense against most reactive aldehydes, including lipid aldehydes (e.g., 4-HNE) and chloroacetaldehyde (VC metabolite). We hypothesize that this defense may play a key role in the interaction between HFD and VC in fatty …

Epidermal Growth Factor-Like Ligands Regulate Dimer Selection., Jamie S. Rush

Electronic Theses and Dissertations

There are thirteen known endogenous EGF-like ligands. We previously reported that Betacellulin (BTC) increases ligand-mediated corneal wound healing more than Epidermal Growth Factor (EGF) [Peterson et al. (2014) IOVS 55(5):2870-80], although the molecular reason for this is unknown. Despite being better at promoting wound healing via enhanced cell migration, BTC has reduced receptor affinity and weaker induction of EGFR phosphorylation. These data indicate that BTC’s response is not due to enhanced affinity or EGFR-kinase activity. Receptor phosphorylation and proximity ligation assays indicate that BTC treatment significantly increases ErbB3 phosphorylation and EGFR:ErbB3 heterodimers. BTC traffics EGFR at a faster rate than …

Synthesis And Characterization Of Targeted Bar Encapsulated Polylactic-Co-Glycolic Acid Nanoparticles To Inhibit Porphyromonas Gingivalis Biofilm Formation., Ranjith Radha Krishnan

Electronic Theses and Dissertations

Periodontal disease is one of the most prevalent infectious diseases worldwide. Between 30-50% of the global adult population suffers from periodontal disease. Some form of periodontitis is present in 46% of American adults, corresponding to annual expenditures in excess of 14 billion dollars for treatment and prevention. Current treatments for periodontal diseases involve mechanical removal of plaque, correction of risk factors, gingival surgery and/or antibiotic therapy. To our knowledge there is no effective therapeutic approach that aims to limit pathogen colonization of the oral biofilm or re-colonization after treatment. Interaction of the pathogen Porphyromonas gingivalis with oral streptococci is critical …

Landmine Detection Using Semi-Supervised Learning., Graham Reid

Electronic Theses and Dissertations

Landmine detection is imperative for the preservation of both military and civilian lives. While landmines are easy to place, they are relatively difficult to remove. The classic method of detecting landmines was by using metal-detectors. However, many present-day landmines are composed of little to no metal, necessitating the use of additional technologies. One of the most successful and widely employed technologies is Ground Penetrating Radar (GPR). In order to maximize efficiency of GPR-based landmine detection and minimize wasted effort caused by false alarms, intelligent detection methods such as machine learning are used. Many sophisticated algorithms are developed and employed to …

Particulate Hexavalent Chromium Does Not Induce Centrosome Amplification In Sperm Whale And Bowhead Whale Cells., Jennifer Haruka Toyoda

Electronic Theses and Dissertations

Hexavalent chromium [Cr(VI)] is a widespread environmental and occupational carcinogen. The mechanism of carcinogenesis remains poorly understood, but chromosome instability (CIN) is the dominant theory. How numerical CIN arises is unclear, but it correlates with centrosome amplification. Both phenotypes are hallmarks of cancers, early events in carcinogenesis, and have been shown to occur after Cr(VI) exposure to human skin and lung fibroblasts. In this study, we investigate numerical CIN and centrosome amplification in whale cells. Whales are our closest marine relatives, have long lifespans, and are exposed to environmental Cr(VI). Importantly, they have low cancer rates and cell culture studies …

Direct And Indirect Effects Of Invasive Cirsium Arvense On Pollination In Southern Appalachian Floral Communities, Jesse Daniels

Electronic Theses and Dissertations

Invasive plants can alter pollination dynamics in invaded communities by disrupting patterns of pollinator visitation, pollen transfer dynamics (conspecific [CP] and heterospecific [HP]), and reproductive success. The direction of invasive effects (competitive, neutral, and facilitative) may be partially determined by spatial scale and species’ floral traits. Here, we investigated pollinator visitation, CP and HP receipt, and pollen tube growth for species in a C. arvense present community and non-present community at two scales. At the community-level, the effect of C. arvense on pollinator visitation varied among species. Floral symmetry seemed to explain this variation. At the floral neighborhood-level, we found …

Localization Of Sip470, A Plant Lipid Transfer Protein In Nicotiana Tabacum, Shantaya Andrews

Electronic Theses and Dissertations

SABP2-interacting protein 470 (SIP470), a non-specific lipid transfer protein (nsLTP), was discovered in a yeast two-hybrid screening using SABP2 as bait and tobacco leaf proteins as prey. SABP2 is an important enzyme in systemic acquired resistance that converts salicylic acid to methyl salicylate. Localization studies are an important aspect to understanding the biological function of proteins. nsLTPs are generally considered apoplastic proteins and has been localized intracellularly and extracellularly. Transient expression shows highest expression of SIP470-eGFP at 2 days post infiltration into Nicotiana benthamiana. Confocal microscopy showed localization near the periphery of the cell. Subcellular localization using differential centrifugation showed …

Early-Childhood Computer-Based Testing: Effects Of A Digital Literacy Intervention On Student Confidence And Performance, Benjamin-David Christopher Legrand

Electronic Theses and Dissertations

Early-childhood digital students grow up in a fast-evolving age of technology requiring them to use and create with technologies and demonstrate core content knowledge. Although third grade students are mandated to master a new language of standardized testing, a large percentage must also learn a language of technology to complete new computer-based tests to measure content mastery. Krashen (1982) defines high affective filter as negative emotional/motivational factors interfering with understanding and cognition. This high affective filter reduces confidence and negatively impacts measuring content mastery on new computerbased tests. Two third grade classrooms at a high-poverty metropolitan school participated in a …

Bluegrass And Old-Time In Catalonia: An Ethnographic Case Study Of Aesthetic Communitas, Michael J. Luchtan

Electronic Theses and Dissertations

This is an ethnographic case study of a musical community in Catalonia centered around the performance of bluegrass and old-time music. By using Victor and Edith Turners’ ideas of normative communitas, this paper identifies an aesthetic communitas model which describes a community centered around a performative genre. Through participant observation in the 16th Annual Al Ras Bluegrass and Old Time Music Festival and interviews with local musicians, fans, venue owners, and luthiers, the ethnographic narrative details the characteristics of the aesthetic communitas in Catalonia and searches for associations of Appalachia that accompany the cross-cultural manifestation of bluegrass and old-time music …

The Impact Of Foreign Aid On Extreme Poverty: A Case Study Of Liberia’S Development Complexities (1980-2018), Roosevelt Seedee

Electronic Theses and Dissertations

Many countries in West Africa, including Liberia, remain trapped in extreme poverty and dysfunctional social services amid continued increase in foreign aid. This study examines complexities influencing decisions of government donors in determining the kind of development assistance needed in Liberia and nation states in West Africa. This research explains the ways in which aid perpetuates poverty instead of alleviating it using interdisciplinary research approaches. Although aid is critical to Liberia’s development agenda, aid implementation faces numerous challenges because of extant poverty and rampant corruption.

Part of the problem in Liberia is not solely the failure of aid to meet …

The Study Of Alcoholic Liver Diseases, Seun Emmanuel Owoseni

Electronic Theses and Dissertations

Excessive alcohol consumption is the primary contributing factor in the development of alcoholic liver diseases (ALD). Nicotine contained in tobacco is a major addictive alkaloid, which enhances the effects of ALDs. The major enzyme involved in nicotine metabolism is cytochrome P450 2A5 (CYP2A5) which is produced in the liver. Alcohol can stimulate the CYP2A5 enzyme. We utilized cyp2a5-/- knockout mice in this research to examine the effects of CYP2A5.

The cyp2a5-/- mice and wild-type (WT) mice were fed liquid ethanol diet with or without nicotine to induce ALD. Nicotine enhancing effects on ALD were observed in WT mice but not …

The Role Of Cyp2a5 In Cadmium-Induced Liver Injury, Julia Salamat

Electronic Theses and Dissertations

Cadmium is present in food and groundwater. Tobacco smoking and occupational exposure are also major sources for cadmium. Cadmium is primarily accumulated in liver, a major organ metabolizing exogenous chemicals. Chemical metabolism may cause detoxification, but it can also cause bio-activation resulting in liver damage. Cytochrome P450s (CYP) are major liver metabolism enzymes, and cadmium chloride (CdCl2) can induce CYP2A5 in mice. We examined the effect of CYP2A5 on CdCl2-induced liver injury using CYP2A5-knockout (cyp2a5-/-) mice. The cyp2a5-/- mice and their control WT mice were injected CdCl2 intraperitoneally at 5 mg/kg body weight, respectively, to induce liver injury. …

Perceptions Of Confidentiality And Stigma Associated With Use Of Counseling Services, Jesi Hall

Electronic Theses and Dissertations

Counseling services are offered in most universities, but are often underutilized. Past researchers found that students listed time, cost, stigma, and confidentiality concerns as their top reasons for avoiding seeking help (e.g., Clement et al., 2015; Dearing, Maddux, and Tangney, 2005). The purpose of this study was to identify whether concerns about confidentiality affect stigma related to use of mental health services. Students answered questions about perceived stigma and the reasons they have avoided seeking counseling in the past. Those who had previously used services reported fewer confidentiality concerns. In a stepwise multiple regression, concerns about confidentiality were found to …

Imidacloprid Persistence, Mobility, And Effect On Ecosystem Function, Joanna Hardin

Electronic Theses and Dissertations

Imidacloprid is a neonicotinoid pesticide used to protect against biting and sucking insects. Land managers rely on its systemic properties, however long-term studies investigating imidacloprid effects on ecosystem function are limited. This study investigated imidacloprid applications to Tsuga caroliniana and Tsuga canadensis over time and compared concentrations to measures of ecosystem function including soil respiration, microbial function, and invertebrate density. Results indicate that imidacloprid is persistent (p0.5). Microbial function and invertebrate density were not significantly different between control and treatment locations nor did imidacloprid concentrations correlate with ecosystem functional indicator activity (p>0.05). It is evident that imidacloprid does not …

Epidermal Growth Factor Receptor (Egfr) Inhibition By Polychlorinated Biphenyls Contributes To Non-Alcoholic Fatty Liver Disease (Nafld)., Josiah Hardesty

Electronic Theses and Dissertations

This dissertation describes how poly-chlorinated biphenyls (PCBs) exacerbate the pathogenesis of non-alcoholic fatty liver disease (NAFLD). While PCBs were banned in 1979, they still persist in contaminated biota, including food, and are detected in human plasma and adipose. The body burden of PCBs is associated with elevation of liver enzymes and necrosis markers in humans, characteristic of NAFLD. PCB exposure in high-fat diet fed mice leads to steatohepatitis that recapitulate the findings seen in exposed humans. The global estimate of people diagnosed with NAFLD is up to 1 in 4 people, unrelated to dietary or genetic factors. The hepatic mechanisms …

Characterization Of Sip68 For Its Role In Plant Stress Signaling, Saroj Chandra Lohani

Electronic Theses and Dissertations

Glucosyltransferases catalyze the transfer of glucose molecules from an active donor to acceptor molecules and are involved in many plant processes. SIP68, a tobacco glucosyltransferase protein, is a SABP2-interacting protein. It was identified in a yeast two-hybrid screen using SABP2 as bait and tobacco proteins as prey. SABP2, converts methyl salicylate to salicylic acid (SA) as a part of the signal transduction pathways in SA-mediated defense signaling. Subcellular localization is a crucial aspect of protein functional analysis to assess its biological function. The recombinant SIP68 tagged with eGFP was expressed transiently in Nicotiana benthamiana and observed under confocal microscopy. Fluorescent …

Computational Quantum Chemistry Studies Of The Interactions Of Amino Acids Side Chains With The Guanine Radical Cation., Edward Acheampong

Electronic Theses and Dissertations

Guanine is generally accepted as the most easily oxidized DNA base when cells are subjected to ionizing radiation, photoionization or photosensitization. At pH 7, the midpoint reduction potential is on the order of 0.2 – 0.3 V higher than those of the radicals of e.g. tyrosine, tryptophan cysteine and histidine, so that the radical “repair” (or at least, a thermodynamically favorable reaction) involving these amino acids is feasible. Computational quantum studies have been done on tyrosine, tryptophan, cysteine and histidine side chains as they appear in histones. Density functional theory was employed using B3LYP/6-31G+ (d, p) basis set to study …

“I’Ve Always Identified With The Women:” How Appalachian Women Ballad Singers’ Repertoire Choices Reflect Their Gendered Concerns, Sara Lynch-Thomason

Electronic Theses and Dissertations

This thesis explores how contemporary Appalachian women’s gendered experiences influence their choices of ballad repertoire. This inquiry is pursued through a feminist analysis of interviews with six women ballad singers from Madison County, North Carolina. In evaluating the women’s choices of ballads and their commentary on the songs, this thesis draws upon narratological theories as well as concepts from Appalachian traditional music studies.

This study finds that women’s repertoire preferences reveal contemporary female concerns for physical safety and political agency. The singers also extract hidden transcripts from ballad texts and use ballads to educate audiences about women’s historic oppression. However, …

Comparisons Between Movement Onset Identification Methods Used In Isometric Mid-Thigh Pull Test, Junshi Liu

Electronic Theses and Dissertations

This dissertation aimed to explore the usefulness of using force derivatives for onset detection in the isometric mid-thigh pull test. First, we examined applications of three differential calculus principles, first and second derivative, and curvature using visual detection as a reference under different baseline conditions. Second, we compared the best derivative method to a threshold-based method using visual detection as a reference. Results of our first investigation showed trivial differences between many differential calculus methods and visual detection. However, statistical differences exceeding a trivial effect was observed when instantaneous force and rate of force develop were examined. Through the first …

Political Competition And Predictors Of Hate Crime: A County-Level Analysis, Eaven Holder

Electronic Theses and Dissertations

Research on hate crime has tended to utilize sociological frameworks to best explain the incidence of such offending, but little research has been conducted to determine whether political factors may play a role. Although Olzak (1990) touched upon the relationship between racial violence and third-party politics during the American Progressive era (1882-1914), the research did not fully articulate how political competition may influence the commission of hate crime. The current study seeks to fill this gap, while also extending concepts associated with social disorganization theory and the defended communities perspective. It does so by utilizing a longitudinal research design to …

Undergraduate Student Perceptions Of Ap And Dual Enrollment In Relation To College Readiness Skills, Ashleigh E. Norris-Shu

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if undergraduate students perceived that their college readiness was impacted by participation in Advanced Placement courses, dual-enrollment courses, or both. Perceptual data were gathered from freshmen and sophomores enrolled at East Tennessee State University using an online survey. The number of participants in this study was 265. Perceptions of the individual program components of both Advanced Placement and dual-enrollment courses were also assessed in relation to college readiness. The results of the study indicated that undergraduate students perceive instructor quality and course rigor of both AP and dual-enrollment as beneficial to …

Self-Compassion And Physical Health-Related Quality Of Life In Cancer: Mediating Effects Of Control Beliefs And Treatment Adherence, Morgan Treaster

Electronic Theses and Dissertations

Among the 14 million persons living in the United States with current or remitted cancer, poor physical health-related quality of life (HRQL) is a significant concern. However, self-compassion (i.e., common humanity, mindfulness, self-kindness) may be a protective factor, either directly or indirectly, by allowing for a sense of empowerment and control over illness, and in turn, facilitating engagement in treatment and positive perceptions of health. Serial mediation analyses among persons living with current (n = 67) or remitted (n = 168) cancer lend support for a positive, direct association between self-compassion and physical HRQL, as well as indirect effects via …

Gaming Behaviors In Day-To-Day Life: Exploring A Problematic Gaming Behavior Scale, Christin Collie

Electronic Theses and Dissertations

Growing concern for the problematic use of video games has prompted new research in a now growing field of literature. Internet Gaming Disorder (IGD) is a proposed behavioral addiction in the Diagnostic and Statistical Manual of Mental Disorders, fifth edition (DSM-5). To examine the proposed criteria for IGD, a self-report problematic gaming behavior scale (PGBS) was developed for adults residing in the United States. One hundred eighty-nine participants completed an online questionnaire comprised of demographic items, general gaming information, the PGBS, and the Internet Gaming Disorder-20 Test (IGD-20 Test). Statistical analyses conducted at the conclusion of the study were consistent …

Basic Academic Skills And Post-Secondary Technical Education, Janet Humphreys Latimer

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if there is a significant difference in WorkKeys score, skills score, theory score, and job placement rates as compared by credential and program of study at a technical college in Tennessee. The study used data retrieved from a WorkKeys database and SIMS (Student Information Management System) at a technical college. The population consisted of 445 students in seven programs from 2010-2016 who had participated in the WorkKeys online academic training modules.

The dependent variables for the study were WorkKeys score, skill score, and theory score. The independent variables were job placement …

Worry In The Pews: Exploring Levels And Causes Of Worry Among Church-Goers, Jennifer Lee

Electronic Theses and Dissertations

A large body of research indicates that worry, anxiety, and depression are present in the modern American psyche at increasingly high levels. Everyday worry can lead to further mental health issues, interpersonal problems, and reduced physical wellbeing and should be reduced when possible. Previous research has examined the benefits of church attendance with varied results; however, understanding the relationship between church attendance and everyday worry could be useful in reducing the effects of worry on this population.

The purpose of this quantitative study was to measure self-reported levels and causes of everyday worry among church-goers from three congregations and to …

A Normal Form For Words In The Temperley-Lieb Algebra And The Artin Braid Group On Three Strands, Jack Hartsell

Electronic Theses and Dissertations

The motivation for this thesis is the computer-assisted calculation of the Jones poly- nomial from braid words in the Artin braid group on three strands, denoted B3. The method used for calculation of the Jones polynomial is the original method that was created when the Jones polynomial was first discovered by Vaughan Jones in 1984. This method utilizes the Temperley-Lieb algebra, and in our case the Temperley-Lieb Algebra on three strands, denoted A3, thus generalizations about A3 that assist with the process of calculation are pursued.