Digital Commons Network^™

Ethical Oversight Of Roles & Relationships In Treatment & Research In Advanced Disease States, Alexa Vercelli

Electronic Theses and Dissertations

There is often ethical analysis focusing solely on the care of the patient by the physician or on the protection of the research participant by the clinical investigator, but those two are often overlapping. By paralleling the analysis of treatment and research, it emphasizes the specific ethical approaches necessary for individuals facing advanced diseases states in the separate roles of being a patient or participant. It also gives guidance to the complexity of the ethics surrounding medical professionals who float between the roles of treating physicians and clinical investigators. As individuals with advanced disease states are considered a vulnerable population …

Respecting The Ethical Tension Between Surveillance And Privacy In Promoting Public Health And Disease Management, Kamal Althobaiti

Electronic Theses and Dissertations

The recognition of the need to undertake surveillance and to protect privacy is well established. However, the continually changing circumstances and fast-paced development of healthcare today requires a continuing need to respect this ethical tension between surveillance and privacy. Hence, this dissertation is to respect the ethical tension between surveillance and privacy in promoting public health and disease management. This dissertation investigates the ethics of conducting public health surveillance, including the challenges associated with obtaining consent and protecting data from unauthorized access. The dissertation will focus on the ethical consequences of big data, including issues associated with obtaining informed consent, …

Quality By Design Based Optimization Of Anti-Inflammatory Nanoemulgels For Local Treatment Of Burn Wounds, Leah Dickey

Electronic Theses and Dissertations

Burns are some of the most traumatizing and damaging skin injuries, with the potential of causing extensive internal damage that increases the risk of chronic wound development. Oral and topical analgesics in combination with antibiotics can provide temporary relief and improve wound healing progress for both mild and severe burns. However, recurrent systemic exposure to pain relievers and antibiotics has been associated with adverse side effects including nausea, drowsiness, abdominal pain, etc., and increased risk of new infections or even ulcers. Local and targeted drug delivery systems, such as nanomedicine, have gained attention as potentially more effective alternatives to improve …

Novel Signal Sequences And Fusion Partners For Paratransgenesis In Asaia, Christina Grogan

Electronic Theses and Dissertations

Mosquitoes transmit many pathogens that cause human disease. One such disease, malaria, is caused by parasites in the genus Plasmodium, infecting over 200 million people and killing over 600,000 per year. Current strategies to control vector-transmitted diseases are increasingly undermined by mosquito and pathogen resistance. Research has turned to additional and novel methods of control, such as altering the microbiota of the vectors. In this method, called paratransgenesis, symbiotic bacteria are genetically modified to affect the mosquito’s phenotype by engineering them to deliver antiplasmodial molecules into the midgut to kill parasites. These molecules must be released by the …

The Effects Of Digital Game-Based Instruction On Knowledge Acquisition And Retention Of Genetics Content, Brinley Kantorski

Electronic Theses and Dissertations

Digital educational games have been a part of the educational landscape since the early 1970s. Since then, there has been a proliferation in the quantity, format, type, and quality of digital educational games available to classroom teachers. Despite this, the body of literature surrounding the effectiveness of these digital educational games remains highly fragmented and lacks unifying themes. While some literature exists to support the use of digital educational games in immediate knowledge acquisition, little research has been done on how digital educational games impact the retention of knowledge in the long term. As such, this study examines how the …

Identification And Characterization Of Genetic Elements That Regulate A C-Di-Gmp Mediated Multicellular Trait In Pseudomonas Fluorescens, Collin Kessler

Electronic Theses and Dissertations

Microbial communities contain densely packed cells where competition for space and resources are fierce. These communities are generally referred to as biofilms and provide advantages to individual cells against immunological and antimicrobial intervention, dehydration, and predation. High intracellular pools of cyclic diguanylate monophosphate (c-di-GMP) cause cells to aggregate during biofilm formation through the production of diverse extracellular polymers. Genes that encode c-di-GMP catalytic enzymes are commonly mutated during chronic infections where opportunists display enhanced resistance to phagocytosis and antibiotics. Our lab uses an emergent multicellular trait in the model organism Pseudomonas fluorescens Pf0-1 to study the emergence of c-di-GMP mutations …

'Bleeding-In-The-World': A Qualitative Study Of Self-Cutting And Blood, Sadie Mohler

Electronic Theses and Dissertations

This dissertation is an existential, phenomenological study of the role and meaning of blood in self-cutting. Using in-depth, open-ended interviews with participants who self-cut, the author gathered data on the multisensorial experience of blood and bleeding in self-cutting. Data analysis was organized around the lifeworld existentials of corporeality, temporality, spatiality, communication, and relationality. The impact of blood and bleeding across each lifeworld dimension emphasized highly relevant, and previously unstudied, aspects of the lived experience of self-cutting. The six themes identified and explored are (a) blood as an animate abject; (b) bleeding and control; (c) bleeding is a process and the …

Promoting Trauma-Informed Competencies In Public School Teachers And Staff, Stephanie Stern

Electronic Theses and Dissertations

Childhood trauma is prevalent among today’s students. When educators implement trauma-informed practices in schools, student behaviors and learning typically improve. It is thus important that educators are trained in and have positive attitudes, knowledge, and beliefs toward trauma-informed care. However, high proportions of educators report that they do not receive trauma-informed training, and research regarding the effectiveness of professional development in trauma-informed care is limited. The present study examined whether educators’ knowledge, attitudes, and beliefs regarding trauma-informed care differed by grade level of students worked with and previous professional development experiences in trauma-informed care, as well as tested the effectiveness …

“I Hear You”: Exploring The Lived Experience Of Counselors’ Empathic Response To Clients When Conducting Telephonic Counseling During Covid-19, Michael Sickels

Electronic Theses and Dissertations

Dissertation supervised by Dr. Debra Hyatt-Burkhart

This study sought to unearth the lived experience of counselors empathically responding to clients via telephonic counseling during COVID-19. The term empathic response refers to as attending to the emotional and mental state of another person in a way that is attuned with the feelings and meanings of the individual’s experience. There are few studies that have assessed the merits of telephonic counseling, and even fewer that have examined counselors’ empathic response to clients through this medium.

To uncover the lived experience of the target population, the participants of the study were purposefully selected …

Rendering Documentary Portraiture: An Interrogation Of Archival Discourse Through A Critical Exploration Of Nineteenth Century Stage Actress Charlotte Cushman’S Material Memory, Skyler Sunday

Electronic Theses and Dissertations

Visual depictions of nineteenth century stage actress Charlotte Cushman, such as photographs, engravings, and painted portraits assist researchers in re-envisioning her both as an actress and as a person, but what do her remaining archival possessions further reveal to researchers about her memory? How do different objects operate as portraits that allow the researcher to tap into and remember specific moments and memory? How does the effort to preserve memory take different forms? This project argues that, when viewing the archive through its stored objects, our collective notion of portraiture can be expanded and used to interrogate existing methods of …

The Effectiveness Of An Evidence-Based Performance Feedback Intervention Through Zoom Communications, Inc., Kristen Mahony

Electronic Theses and Dissertations

The current study was conducted during the COVID-19 pandemic after the American Psychological Association (APA) called for modified research practices to ensure the safety of students and families. The evidence-based writing fluency intervention known as the Performance Feedback (PF) Intervention was implemented via a fully remote platform. A single-subject multiple-baseline across participants design was utilized to evaluate the effects of the adapted intervention on the writing skills of three elementary-aged students with disabilities while also measuring maintenance of those skills two weeks following the intervention. Additionally, researchers measured the social acceptability of the virtual PF intervention by the students. Visual …

Neural Stem Cells: Age-Dependent Outcomes During Viral Infections In The Central Nervous System, Manisha N. Chandwani

Electronic Theses and Dissertations

Viral infections in the central nervous system (CNS) are associated with neurodevelopmental and neurobehavioral deficits. The outcomes of viral infections can be driven by damage and death of neurons. Neural stem cells (NSCs) play key roles in neurodevelopment, repair, and physiological brain function. During a viral infection, NSC activity can disturbed by direct infection of NSCs by the virus or by anti-viral immune response. Here, we aimed to assess whether the anti-viral immune response can impact NSC activity during an immunocompetent response in the adult brain. We utilized a transgenic mouse model of Measles virus infection where only the CNS …

Social Justice Informed School Counseling: A Conceptual Model For Promoting Academic Achievement And Equity Among Dual-Enrolled African American Students, Sylvester Hanner

Electronic Theses and Dissertations

To promote college enrollment, high schools may offer students the opportunity to participate in dual enrollment programs. Students who gain college credit while earning a high school diploma may be more likely to graduate college on time and at a lower cost (College Board, 2017). Despite the benefits of dual enrollment, African American students lack equitable access to dual enrollment programs. Additionally, when they are afforded access to participate in dual enrollment courses, they have a lower academic success rate when compared to White students (CCRC Fink, 2017).

This study provides insight into concepts related to African American students’ opinions, …

Labeling Melanoma Cells With Black Microspheres For Improved Sensitivity In Detection Via Photoacoustic Flow Cytometry, Tori Kocsis

Electronic Theses and Dissertations

Melanoma is an aggressive form of skin cancer known for developing into metastatic disease. Current clinical diagnostics, including medical imaging and tissue biopsy, provide a poor prognosis since the cancer is in the late stages of disease progression. In recent years, photoacoustic flow cytometry has allowed for the detection of circulating melanoma cells within patient blood samples in vitro. Although this method exploits the naturally-produced melanin within the cells, it has only successfully detected highly-pigmented melanoma cell lines. Since various forms of melanoma exist, each with varying melanin concentrations, this research aims to provide a novel method for detecting lightly-pigmented …

Geochemical Characterization Of The Louann Salt, Puma Field, Green Canyon, Gulf Of Mexico Basin – Using Xrf And Xrd, Brian Lesh

Electronic Theses and Dissertations

The Jurassic-aged Louann Salt is an evaporite sequence deposited in the Gulf of Mexico Basin (GOMB). It is an important component of the GOMB petroleum system and influences the facies pattern in overlying strata and the distribution of petroleum stratigraphic traps. The Louann Salt is composed primarily of halite with intercalated anhydrites and silty or sandy-halite intervals, with overall thicknesses in excess of four kilometers (~13,000 feet). Suture zones occur intermittently within the Louann Salt, formed by the collision of allochthonous evaporite layers. The mineralogy and geochemistry of the suture zones are currently understudied and can be hazardous during drilling …

Soil Salinity And Sodicity Of Galveston And Pelican Islands, Texas, Rachel Murray

Electronic Theses and Dissertations

Galveston Island, Texas, is frequently affected by hurricanes. In 2008, Hurricane Ike inundated much of the island with seawater, causing elevated soil salinity levels that killed much of the vegetation on the island, including tens of thousands of trees. Residual salinity in soils and groundwater has hampered vegetation re-establishment in some areas.

This project aims to create a map product that displays the current soil salinity and sodicity levels on Galveston and Pelican Islands. The existing USDA Natural Resource Conservation Service (NRCS) Galveston County Soil Survey map units were used as a base for the project. ArcGIS Desktop software was …

Geochemical Characterization Of The Bone Spring Formation, Delaware Basin, Using Chemostratigraphy And Integrated Petrophysics, David Tonner

Electronic Theses and Dissertations

The Delaware Basin forms part of West Texas's and New Mexico’s famous petroleum-generating Permian Basin. The Bone Spring Formation is a prolific hydrocarbon producer within this basin, creating one of the world’s richest oil shales. This formation has lithological sequences that are characterized by repeating carbonate and siliciclastic intervals of a third-order cycle which can largely be correlated to highstand and lowstand systems tracts, respectively. Lithological complexity and facies change are manifested by debris flows, turbidites, and slumps. In addition to glacio-eustasy, both tectonism and broader Milankovitch cycles have influenced the depositional history. Previous investigations have utilized cores and wireline …

Translating Particulate Hexavalent Chromium-Induced Chromosome Instability From Human Lung Cells To Experimental Animals, Human Lung Tumors And Whale Cells., Haiyan Lu

Electronic Theses and Dissertations

Lung cancer is a major human health problem. While smoking is the most well-known cause of lung cancer, people who never smoked develop the disease. Understanding how non-tobacco environmental carcinogens cause lung cancer is key to combating this disease. Hexavalent chromium [Cr(VI)] is a well-established human lung carcinogen, but its carcinogenic mechanism is uncertain. Chromosome instability (CIN) is a hallmark of lung cancer and a major factor in Cr(VI)-induced lung cancer. Studies in human lung cells show Cr(VI) induces DNA double strand breaks and suppresses homologous recombination (HR) repair by targeting RAD51, resulting in CIN. We translated these outcomes to …

Optimal Scheduling Of Connected And Autonomous Vehicles At A Reservation-Based Intersection., Muting Ma

Electronic Theses and Dissertations

Reservation-based intersection control has been evaluated with better performance over traditional signal controls in terms of intersection safety, efficiency, and emission. Controlling connected and autonomous vehicles (CAVs) at a reservation-based intersection in terms of improving intersection efficiency is performed via two factors: trajectory (speed profile) and arrival time of CAVs at the intersection. In an early stage of the reservation-based intersection control, an intersection controller at the intersection may fail to find a feasible solution for both the trajectory and arrival time for a CAV at a certain planning horizon. Leveraging a deeper understanding of the control problem, reservation-based intersection …

Voices Of The "Served": Community Resident Perspectives On International Service-Learning In Peru., Adam C. Stieglitz

Electronic Theses and Dissertations

The purpose of this study is to capture and share community resident perspectives on international service-learning (ISL). Community resident voice is largely underrepresented in ISL scholarship and practice. Conversely, research on student outcomes is ubiquitous, which generally makes student benefit a predominant consideration in ISL program design. Consequently, scholars have criticized ISL programs for being inherently extractive and even an embodiment of modern-day colonialism. This study aims to address this critique by offering a robust representation of community residents’ perspectives for how to responsibly design ISL programs. This study used action research methodology to capture community resident voices who participate …

The Effects Of Gang Membership And Transitions In A Gang On Within-Individual Changes In Offending., Leon Sergio Garduno

Electronic Theses and Dissertations

Researchers have established that joining a gang exposes individuals to factors and environments that promote and increase antisocial and illegal behaviors, which decrease after leaving the gang (Decker & Van Winkle, 1994; Melde & Esbensen, 2014; Thornberry, Krohn et al., 1993). Scholars, by analyzing qualitative data, bivariate statistics, and by comparing gang members with non-gang members, have found that as individuals join and leave a gang they experience changes in the frequency with which they carry firearms, sell drugs, and engage in property crimes (Bjerregaard & Lizotte, 1995; Hagedorn, 1994; Thornberry et al., 1993). Researchers also have established that within-individuals’ …

Applications Of Nuclear Magnetic Resonance Spectroscopy: From Drug Discovery To Protein Structure And Dynamics., Mark Vincent C. Dela Cerna

Electronic Theses and Dissertations

The versatility of nuclear magnetic resonance (NMR) spectroscopy is apparent when presented with diverse applications to which it can contribute. Here, NMR is used i) as a screening/ validation tool for a drug discovery program targeting the Phosphatase of Regenerating Liver 3 (PRL3), ii) to characterize the conformational heterogeneity of p53 regulator, Murine Double Minute X (MDMX), and iii) to characterize the solution dynamics of guanosine monophosphate kinase (GMPK). Mounting evidence suggesting roles for PRL3 in oncogenesis and metastasis has catapulted it into prominence as a cancer drug target. Yet, despite significant efforts, there are no PRL3 small molecule inhibitors …

Determination Of The Functional Role Of Rab-Ggt In Physcomitrium Patens., Hyun Jin Jung

Electronic Theses and Dissertations

Protein prenylation, a common lipid post-translational modification, is required for growth and development in eukaryotes. Rab geranylgeranylation involves the addition of one or two 20-carbon geranylgeranyl moieties to Rab-GTPase target proteins, which regulate intracellular vesicle trafficking. The reaction is carried out by heterodimeric Rab geranylgeranyltransferase (Rab-GGT), which is composed of two associated α- and β-subunits, with the assistance of an additional protein called Rab escort protein (REP). Loss of function of the Rab-GGT α subunit RGTA1 has not been reported in any plant. While knockout of either of the two β subunits RGTB1 or RGTB2 results in …

Effect Of Dorsal Quadrant Or Ventral Quadrant Spinal Cord Injury On Gait Features During Locomotion., Anya Nicole Trell

Electronic Theses and Dissertations

In the Unites States, approximately 1.5 million people currently have a spinal cord injury and suffer permanent sensory and motor loss due to the disruption of the spinal cord. Due to the significant morbidity, it is vital to understand the functional impact of disrupting neural descending pathways that modulate spinal neurons involved in intermuscular coordination critical for gait behaviors. Tasks that are more difficult require additional input from these neural pathways; therefore, fourteen feline subjects were familiarized with level overground locomotion and stair descent gait tasks. After collection of baseline kinematic data, the subjects received either a dorsal or ventral …

Targeting Pathological Changes In The Gut Protects The Cns From Neuropathology., Benjamin Tyler Charpentier

Electronic Theses and Dissertations

There is growing evidence that the health of the gut microbiome (GM) has an important role on multiple host organ systems. Ethanol-related neuropathologies and Alzheimer’s disease (AD) currently have no effective clinical treatment. Therefore, we developed a novel mouse model of fecal matter transplantation (FMT) utilizing the microbiome from alcoholic hepatitis (AH) patients, characterized by a loss of butyrogenic potential. Minimal neuroinflammation occurred in AH-FMT mice, but significant increases in the endoplasmic reticulum stress response (ERSR) across the majority of neurons in the prefrontal cortex and hippocampus were observed. In the cerebellum, Purkinje cells were specifically affected. Targeting the loss …

Classifying The 2022 Status Of Tsuga Canadensis (Eastern Hemlock) Along The Kentucky Portion Of The Pine Mountain Wildlands Corridor., Grace M. Embree

Electronic Theses and Dissertations

The invasion of the Hemlock Woolly Adelgid (HWA) (Adelges tsugae) has posed a continual threat in the United States to the Eastern Hemlock (Tsuga canadensis) trees since the 1950s. HWA feed on eastern hemlock needles, reducing the amount of healthy photosynthesizing vegetative area. The use of satellite imagery has been instrumental in identifying areas of eastern hemlock presence. Satellite platforms like Landsat and AVIRIS are commonly used for identification, classification, and mapping of eastern hemlock. Sentinel-2 imagery was released in 2015 for free access. It has a finer spatial grain of with the majority of the …

The On-Line Width Of Various Classes Of Posets., Israel R. Curbelo

Electronic Theses and Dissertations

An on-line chain partitioning algorithm receives a poset, one element at a time, and irrevocably assigns the element to one of the chains. Over 30 years ago, Szemer\'edi proved that any on-line algorithm could be forced to use $\binom{w+1}{2}$ chains to partition a poset of width $w$. The maximum number of chains that can be forced on any on-line algorithm remains unknown. In the survey paper by Bosek et al., variants of the problem were studied where the class is restricted to posets of bounded dimension or where the poset is presented via a realizer of size $d$. We prove …

Experimental Evaluation Of Heat Leak From Anti-Sweat Components Into Fresh Food And Freezer Compartments Of A Household Refrigerator., Delva Felissaint

Electronic Theses and Dissertations

Condensation beads forming on outside surfaces around a refrigerator is an undesirable byproduct driven by the cold temperature exhibited throughout the unit. To combat this issue, anti-sweat components usually in the form of electrical heaters or tubes carrying hot refrigerant, bring heat to the cold surfaces to ensure their temperatures do not drop below the dew point. Some heat produced by the anti-sweat components may transfer to the interior refrigerated compartment. The purpose of this study is to quantify how much heat from the anti-sweat components are entering the fresh food and freezer compartments and what impact that has on …

Meeting The Mental Health Needs Of College-Aged Young Adults: Evaluating The Value And Impact Of Digital Mental Health Interventions., Sara Atherton Choate

Electronic Theses and Dissertations

BACKGROUND: The growing prevalence of common mental health problems poses a serious hindrance to young adults, and the majority of those in need of mental health support do not seek professional psychological services. Digital mental health interventions (DMHIs) that allow for self-management of mental health symptoms could provide a useful adjunctiveto traditional one-on-one counseling or therapy and offer a useful prevention tool for students at risk for experiencing mental health crises. This dissertation explored the efficacy of self-guided DMHIs targeting college students, the factors associated with help-seeking in young adults, and the impact of implementing an evidence-based DMHI at a …

Search For A Dark Leptophilic Scalar Produced In Association With Taupair In Electron-Positron Annihilation At Center-Of-Mass Energies Near 10.58 Gev., Diptaparna Biswas

Electronic Theses and Dissertations

Dark matter is believed to be a form of matter which seemingly accounts for approximately 85% of the matter in the universe and about 27% of its total mass–energy density. It doesn't participate in electromagnetic interaction, i.e. doesn't interact with light. Consequently, we cannot see it using optical or radio telescope and hence the name dark matter. However, it participates in gravitational interaction, and we hypothesize its existence based on a variety of astrophysical observations, including gravitational effects, that cannot be explained by the accepted theories of gravity unless we account for more matter than can be perceived through electromagnetic …