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Mapping The Binding Site Within Integrin D2 For Carboxyethylpyrrole (Cep)-Modified Proteins, Afia Prema

Electronic Theses and Dissertations

Neutrophils and macrophages accumulate at sites of inflammation and cause chronic inflammation leading to various diseases. Therefore, to better understand chronic disease pathways it is important to investigate the properties of macrophage accumulation in inflamed tissues. The I-domain of the macrophage receptor integrin a_Db₂ plays a vital role in macrophage retention by binding to CEP (carboxyethyl pyrrole), a ligand available at inflammatory sites. This thesis mainly focuses on evaluating the binding site within integrin a_Db₂ that binds carboxyethyl pyrrole (CEP)-modified proteins. So, a recombinant plasmid construct containing the integrin I-domain was developed. Seven non-conserved …

Sound And Music Opportunities In Toddler Learning Environments, Sonia Akiko Yoshizawa

Electronic Theses and Dissertations

The aim of this qualitative research was to gain an in-depth understanding of how teachers of toddlers (aged 25-36 months) described sound and music learning opportunities and experiences provided to children in their learning environments. In this research, teachers were asked how their personal and professional experiences with sound and music influenced their planning of toddlers’ sound and music learning opportunities. The design of this study was in-depth interviews of six toddler lead teachers in three childcare sites. Data were gathered through photographs, planning documents, and in-depth interviews using a university IRB-approved video conferencing program. Findings showed that all participants …

Strong As A Mother: The Resilience Of Women Who Have Previously Experienced Infertility, Madison Hinkle

Electronic Theses and Dissertations

Infertility impacts numerous individuals during their reproductive journey. Yet, there is little research and information available that examines if having a history of infertility goes on to have further implications after an individual conceives and gives birth. Thus, the current study aimed to examine if infertility impacts the postpartum period, and if there were any particular risk or resiliency factors that contributed to this relationship. In this study, women aged 18 to 50, who had given birth within the last 12 months, participated in an online survey that assessed a variety of physical and mental health constructs. Overall, results largely …

Acute Responses To Combined Accentuated Eccentric Loading And Rest Redistribution, Sungwon Chae

Electronic Theses and Dissertations

This dissertation was 2 parts of investigations of various acute responses to high-volume combined accentuated eccentric loading and rest redistribution (AEL + RR). Resistance-trained men (n = 12, 25.6 ± 4.4 years, 1.77 ± 0.06 m, 81.7 ± 11.4 kg) completed a back squat (BS) 1 repetition maximum (1RM) and weight releaser familiarization session. Three BS exercise conditions (sets × repetitions × eccentric/concentric loading) comprised AEL + RR 5: 3 × (5 × 2) × 110/60%, AEL + RR 2: 3 × (2 × 5) × 110/60%, and traditional sets (TS): 3 × (1 × 10) × 60/60% 1RM. The …

Impacts Of Urbanization On Pollination Success For Common Milkweed (Asclepias Syriaca), David Rockow

Electronic Theses and Dissertations

Urbanization is among the greatest threats to biodiversity on the planet, and is projected to increase in magnitude in coming years. This growing threat makes it important to better understand how urbanization may impact vital ecosystem services, such as pollination. Studies on the impact of urbanization on pollination vary wildly in their findings. This study analyzed multiple aspects of pollination success in common milkweed populations varying in urbanization level, including insect community composition, pollen removal/deposition, and fruit/seed production. By analyzing multiple pollination aspects we can determine the overall mechanism in which urbanization instills on the entire pollination process. Overall, urbanization …

Navigating Cultural Crossroads: Exploring Fictional And Interview Narratives Of Nigerian Immigrant Women Living In The Southern United States, Tolulope Adeusi

Electronic Theses and Dissertations

Nigerian immigrant women undergo constant navigation of their personal identities when conflicting cultural dynamics sometimes engender a balancing act between their personal beliefs and the ongoing process of acculturation. Their new Southern environment offers its own traditional mores, as well as greater opportunities for economic advancement. This places Nigerian Immigrant Women in a position where they must reconcile their desires for personal independence and empowerment with societal expectations that emphasize more traditional gender roles. This study explores the interview narratives of Nigerian immigrant women, reinforced by fictional accounts from prolific African women writers, which provides a more nuanced discussion of …

Behavioral Indicators Of Reflective Functioning In Mother-Child Dyadic Interactions, Rachel Clingensmith

Electronic Theses and Dissertations

Positive parenting practices and secure attachments are consistently linked to healthy child outcomes (Ainsworth & Bowlby, 1991; Waters et al., 2000). Research on cognitive processes that scaffold parental behaviors which contribute to secure attachment is an essential contribution to the literature, particularly given the potential for early intervention with at-risk families. Parental Reflective Functioning (PRF) is a construct of increasing interest which has been linked to secure attachments and positive child outcomes, with one commonly used self-report measure of PRF being the Parental Reflective Functioning Questionnaire (PRFQ; Camoirano, 2017; Clingensmith, 2021; Luyten et al., 2017). As such, the purpose of …

Geometric Morphometric Analysis Of Modern Viperid Vertebrae Facilitates Identification Of Fossil Specimens, Lance D. Jessee

Electronic Theses and Dissertations

Snake vertebrae are common in the fossil record, whereas cranial remains are generally fragile and rare. Consequently, vertebrae are the most commonly studied fossil element of snakes. However, identification of snake vertebrae can be problematic due to extensive variation. This study utilizes 2-D geometric morphometrics and canonical variates analysis to 1) reveal variation between genera and species and 2) classify vertebrae of modern and fossil eastern North American Agkistrodon and Crotalus. The results show that vertebrae of Agkistrodon and Crotalus can reliably be classified to genus and species using these methods. Based on the statistical analyses, four of the …

Comparison Of The 2022 Monkeypox (Mpox) Outbreak Using Mathematical Modeling And Time Series Clustering, Mark-Daniels Tamakloe

Electronic Theses and Dissertations

Monkeypox virus (MPXV) is the causative agent of monkeypox (mpox), a rare viral disease that affects humans [1]. It is primarily found in Africa and is transmitted to humans through contact with sick animals, particularly rodents and monkeys, or through human-to-human transmission [2]. From the beginning of May 2022, cases of mpox have been recorded from non-endemic nations, and the illness has continued to be reported in other endemic nations. Majority of confirmed cases have been recorded in Europe and North America. In this thesis, we compare the spread of the outbreak across the top ten countries using a combination …

Tennessee Promise And Two-Year Community College Retention And Graduation In Rural Appalachia, Tammy Dycus

Electronic Theses and Dissertations

The purpose of this quantitative, non-experimental study was to explore the relationship between the implementation of the Tennessee Promise scholarship program and the two-year Tennessee community college retention rates and graduation rates of first-time, full-time Tennessee students from rural Appalachian counties. Results from this study may help higher education stake-holders better understand the features of Tennessee Promise that are influencing an increase in community college retention and graduation rates for Tennessee students from rural Appalachian counties. The theoretical framework that guided this research was the social capital framework. Data including use of Tennessee Promise, county of origin, retention, and graduation …

Investigation Of The Protein Components In A Periplasmic Mechanism Regulating Bacterial Morphology, Alexandra Pulliam

Electronic Theses and Dissertations

Salmonella is a leading bacterial cause of foodborne illness worldwide. During a previous study investigating the enzymes responsible for regulating cyclic-di-GMP concentrations, a mutant in the cyclic-di-GMP-specific phosphodiesterase STM3615 was identified that displayed a phenotype characterized by decreased survival on agar plates and a shorter bacterium length. I was able to determine that the periplasmic domain of STM3615 was responsible for this phenotype, not the enzymatic phosphodiesterase domain. Based upon a bioinformatic analysis of the protein, I then hypothesized that the periplasmic domain of STM3615 was interacting with a periplasmic protein to give rise to this phenotype. To identify this …

A Low Cost, Compact Electrochemical Analyzer Based On An Open-Source Microcontroller, Michael Kofi Darko Addo

Electronic Theses and Dissertations

Compared to other instruments for chemical analyses, electrochemical analyzers are relatively simple, inexpensive, easy to miniaturize and require little-to-no maintenance. However, like all commercially available instruments, commercial electrochemical analyzers like potentiostats primarily operate as black boxes with manufacturers providing little or no information about internal circuitry and programming. This practice can limit a researcher’s ability to develop new techniques or adapt an instrument for applications outside its typical use. In contrast, creators of open-source instruments release all the necessary information for reproduction of the hardware and software – minimizing such barriers to innovation in chemical analyses. Here, we report a …

Extraction, Purification, And Characterization Of Potential Bioactive Compounds Produced By Janthinobacterium Lividum Tajx1901, Andy Elorm Agbakpo

Electronic Theses and Dissertations

Underexplored environments such as soil samples continue to be an untapped source of bacterial strains with great potential to produce secondary metabolites for medicinal applications. As a result, these microorganisms represent a broad and yet unknown reservoir of new strains capable of producing these novel compounds. The current research primarily seeks to perform the isolation, purification, and characterization of secondary metabolites from a soil bacterium (Janthinobacterium lividum TAJX1901). The isolated soil bacterium was successfully cultured on rich media agar plates, followed by extraction using methanol and chloroform. The purification methods utilized include flash column chromatography, preparative thin-layer chromatography, and …

An Early Pliocene Fish Assemblage From The Southern Appalachians: Ichthyofauna Of The Gray Fossil Site, Shay Maden

Electronic Theses and Dissertations

The Gray Fossil Site (GFS) preserves an early Pliocene sinkhole pond that accumulated a rich flora and vertebrate fauna. Fossils of fish are incredibly common at GFS, representing thousands of specimens recovered to date. Comparison with extant species reveals a depauperate freshwater fish fauna consisting of only two species: Lepomis sp. and Micropterus sp. The absence of fishes that are ubiquitous in modern streams suggests no perennial surface hydrologic connection between the Gray Fossil Site and the nearby Holston River. Limited opportunities for dispersal into the pond and deleterious conditions such as eutrophy and high competition may have resulted in …

Isolation And Structural Determination Of Bioactive Metabolites From The Soil Bacterium, Arthrobacter Sp. Tajx1902, Amonah Arije

Electronic Theses and Dissertations

As antimicrobial resistance persistently disrupts the treatment of microbial infection, natural product chemistry has played a significant role in identifying novel drugs with novel modes of action critical to getting ahead of resistance. The primary goal of this project is to extract and identify potential novel antimicrobial natural products produced by Arthrobacter sp. Although underexplored, Arthrobacter sps. have been known to produce bioactive compounds with versatility; one such is a depsipeptide with quorum-sensing inhibitory activity.¹ In this research, an Arthrobacter sp. TAJX1902, isolated from a soil sample, showed inhibitory activity against a filamentous indicator bacterium and a violacein-producing Janthinobacterium …

Microsporidian Spores And The Integrin Binding Loop Of The Madam Protein Are Important For Integrin Signaling And Attachment To Host Cells, Cindy L. Barrett

Electronic Theses and Dissertations

Microsporidia are a distant fungal pathogen that have severe clinical consequences for the immunocompromised. Previous work identified a microsporidian pathogen protein termed Microsporidian ADAM or MADAM. This protein has close sequence homology to other ADAM proteins (A Disintegrin and Metalloproteinase) in two microsporidian species, Encephalitozoon intestinalis and E. cuniculi. ADAM proteins have a wide range of functions, including binding to integrins and host signaling. It is known that many pathogens manipulate integrins to invade host cells, and it is predicted that microsporidia are also exploiting this host target. Previous work with the MADAM protein demonstrated that this protein has a …

Overdose Prevention Behaviors And The Rural Risk Environment Among People Who Inject Drugs In Rural Appalachia, Jody Teel

Electronic Theses and Dissertations

Introduction: The entire United States has felt the effects of the overdose epidemic, but rural Appalachia has experienced disproportionate overdose deaths. This variation in overdose mortality can be attributed to the risk environment for overdose in rural Appalachia. Overdose prevention behaviors are individual level behaviors that people who inject drugs can implement that may have the potential to reduce their risk of overdose, however limited research exists regarding the utilization of those behaviors. It is important to consider how the risk environment can influence the utilization of overdose prevention behaviors among people who inject drugs. Therefore, the purpose of this …

Supply Chain Finance: Exploring The State Of Adoption With Small Business Suppliers In U.S. Defense Procurement Contracts, James C. Phelps Iii

Electronic Theses and Dissertations

Innovative supply chain finance (SCF) practices offer alternative approaches to relief of cash flow distress for suppliers in an extended enterprise by enhancing access to and affordability of working capital. Adopting optional SCF instruments can be especially beneficial for small–medium-sized enterprises (SMEs), which are more acutely affected when credit conditions tighten. This explorative research described the state of buyer-led SCF adoption in defense procurement contracts used to produce highly technical weapons systems. A case study research design with embedded units across four groups of intermediaries was employed to compare the state of SCF adoption between a defense and a commercial …

Exploring The Experiences Of Underrepresented Students Pursuing Health-Related Graduate Or Professional Programs, Alison Williams

Electronic Theses and Dissertations

For many years graduate and professional education programs for the health professions have sought to increase the diversity of their student body to include students from a wider variety of backgrounds. Increasing the diversity of healthcare providers is an essential component of addressing inequities in healthcare. However, despite initiatives to increase racial and ethnic diversity in the health professions, these professions remain largely White and female. Previous researchers have sought to identify the reasons that racial and ethnic minorities are underrepresented in healthcare, and the barriers to persistence and success. Little research exists explaining why men are underrepresented in the …

School Leader Perceptions About The Implementation And Utility Of Restorative Practices, Danielle Rutig

Electronic Theses and Dissertations

This phenomenological qualitative study examined school leaders' perceptions of using restorative practices within their schools. Participants included twelve school leaders who had experience with utilizing and implementing restorative practices. School leaders included: principals, assistant principals, deans, and administrative assistants. Research indicates that restorative practices are adapted to support specific school goals. Restorative practices are not practiced in isolation, as school leaders often couple restorative justice with social-emotional learning, trauma-informed practices, and other social-emotional supports. Restorative practices are tied closely to CASEL’s social emotional framework.

Data were collected via semi-structured interviews using a virtual platform. Themes that emerged from the analysis …

Met Alterations In Glioblastoma: Characterization Of Patient-Derived Xenografts And Therapeutic Strategies, Anna Musket

Electronic Theses and Dissertations

Glioblastoma is the most commonly diagnosed central nervous system primary malignancy and it is considered a terminal diagnosis with few treatment options available. Glioblastoma tumors frequently develop treatment resistance due in part to their highly heterogenic nature. The heterogeneity of glioblastoma is partially attributed to the presence of glioma stem-like cells (GSC), which are highly invasive and resistant to chemotherapy and irradiation treatments. Signaling of the receptor tyrosine kinase MET is a known regulator of GSC. Glioblastoma patients have an increasingly poor prognosis that corresponds with increasing MET expression. Both GSC and MET are known to contribute to treatment resistance …

A Pandemic’S Potential To Haunt: A Longitudinal Look At The Professional Wellbeing Of Tn’S Infant And Early Childhood Mental Health (Iecmh) Workforce, Robyn A. Dolson

Electronic Theses and Dissertations

Infant and Early Childhood Mental Health (IECMH) providers engage in uniquely relational work with high-risk families that expose them to the full advantages (i.e., compassion satisfaction [CS]) and disadvantages (e.g., burn-out [BO]) of a helper role. Though the pandemic seemed poised to escalate disadvantage, most early pandemic studies found high CS alongside elevated BO. Unfortunately, as COVID-19 has continued, CS has declined while BO has increased. Given the delayed COVID-19 impact on children, these changes may be particularly acute for IECMH providers. Using a longitudinal cohort of 27 IECMH providers, this study aimed to describe and quantify changes in professional …

Femoral Osteohistology In American Alligators (Alligator Mississippiensis) Reveals High Variation In Growth And Facilitates Interpretation Of An Early Pliocene Alligator, Davis Gunnin

Electronic Theses and Dissertations

Histological analysis of long bone thin sections is commonly used to infer growth rates and ecology of extinct vertebrates, particularly within Archosauria. However, most comparative neontological studies have used small samples of captive individuals, limiting the scope of variation. To fill this gap, 44 femoral thin sections of wild Alligator mississippiensis were prepared and analyzed. Comparison of slides revealed that larger individuals from cooler climates tend to show more LAGs compared to southerly A. mississippiensis of similar size, however, there is considerable variation. This pronounced variation in wild specimens emphasizes the need to use caution when interpreting paleohistological data with …

Lightweight Blockchains And Their Network Impact On Vehicular Ad-Hoc Network-Based Blockchain Applications, Edgar Bowlin

Electronic Theses and Dissertations

Vehicular Ad-hoc Networks (VANETs) provide networks for smart vehicles and will enable future systems to provide services that enhance the overall transportation experience. However, these applications require consideration to possible damage to both property and human life. Communication between vehicles requires data immutability and user privacies to provide safe operation of the system. Blockchains can provide these properties and more to create a more secure and decentralized system. However, a chain’s security comes from the chain length. VANETs’ ephemeral connections provide harm limits how much data can be exchanged during vehicle rendezvous. This thesis investigates lightweight blockchains that operate with …

Age And Paleontology Of The Turin Pit Locality, Monona County, Iowa, Samantha Wright

Electronic Theses and Dissertations

The Turin Pit locality (Monona county, Iowa) has been known to paleontologists since 1908, yet the age of the fauna has been unclear. Early paleontologists considered Turin Pit to date to a pre-Illinoian interglacial (the “Aftonian).” Subsequent researchers suggested it dated to the last glaciation. This study provides a partial list of mammals in the Turin Pit fauna, and together with stratigraphic information, uses the known age ranges of taxa to estimate an age for the assemblage. The presence of Mammuthus, Aenocyon, and Castoroides combined with a magnetically-reversed till located stratigraphically above fossil-bearing deposits, suggest the Turin Pit …

Hands-Only Cpr Education In Tarrant County, Courtnee C. Cross

Electronic Theses and Dissertations

The purpose of this project was to identify the impact of in-person, hands-only CPR education for community members on their confidence and ability in performing CPR. Bystander-initiated CPR is an important aspect in the survival and outcomes of those that experience a cardiac arrest in the community. The hands-only CPR method was developed to help increase bystander-initiated CPR and improve outcomes for those experiencing a cardiac arrest at home. This project was quasi-experimental, using pretest and posttest data. Community members took the Basic Resuscitation Skills Self-Efficacy Scale (BRS-SES) before and after the hands-only CPR education video to determine how the …

The Transformation: Teaching Critical Thinking And Clinical Decision Making In Nursing Education, Winifred Ferguson Adams

Electronic Theses and Dissertations

Abstract

The profession of nursing has recognized in the past 20 years the need for graduate nurses to enter the profession prepared to care for complex medical patients in an ever-changing healthcare environment. It has become a major challenge for nurse educators to provide students with adequate didactic and clinical education in nursing. The overall purpose of this project was to help nurse educators accept the challenge required to make the radical transformation in education. Faculty participated in the measurement of self-efficacy pre and post-exposure to teaching strategies related to (1) critical thinking, (2) clinical judgment, and (3) clinical reasoning …

Effects Of Parental Deployment On Military-Connected High School Students And Strategies For Academic Success, Sean T. Lucas

Electronic Theses and Dissertations

Abstract The problem investigated in this study was unknown opportunities that exist for educators to meet the academic needs of students who are affected by combat parental deployment and how this challenge of combat deployment disrupts the family’s dynamic, thus challenging their children academically. The Air Force, Army, Navy, Marines, and Coast Guard made up about 1% of the total United States population in 2015. Most of these people were married, 42% had children, and 54% of their children were in kindergarten to 12th grade. The Department of Defense school system is comprised of 166 accredited schools across 11 foreign …

The West Point Talent Based Branching Program As A Predictor Of Performance At The Army’S Field Artillery Basic Officer Leadership Course: Implications For Officer Retention, Ryan Oldroyd Scott

Electronic Theses and Dissertations

Junior officer attrition has increased over the last two decades. The early departure of army officers leaves a dearth of middle- and senior-level leadership, creating risk for future military readiness. West Point leaders theorized that new officers’ poor person–job fit within their specific career fields contributed to attrition. West Point implemented a talent-based system to place new officers into branches that should offer a stronger person–job fit, hoping it would reduce officer attrition. To begin the investigation on how this talent-based system affects attrition, this study examined graduates who joined the field artillery career field to ascertain if the various …

The Emotional Intelligence And Conflict Management Relationship In Black Women Leaders, Tina Raylyn Jackson

Electronic Theses and Dissertations

This study examined the relationship between emotional intelligence and conflict management styles in Black women leaders in the United States. The lack of literature surrounding Black women leaders' emotional intelligence or conflict management styles presented an opportunity to offer quantitative data connected to these constructs. A cross-sectional survey design was used to administer three surveys the Situational Test of Emotional Management- B, the Kraybill Conflict Style Inventory, and a modified version the Multidimensional Inventory of Black of Identity, that focused on Black female identity. Data from 124 participants were analyzed to learn if identity mediated the relationship between emotional intelligence …