Digital Commons Network^™

Synthesis Of Bacterial Glycerophospholipids For Biomembrane Model Studies: A Means To Advanced Biofuels, Felix Adulley

Electronic Theses and Dissertations

To reduce reliance on fossil fuels, sustainable biofuels are being pursued, especially advanced biofuels like 1-butanol that have higher energy content and greater compatibility with existing infrastructure than ethanol. A persistent challenge is the yield-limiting toxicity of biofuels and process solvents, such as tetrahydrofuran, to the microbes that ferment biomass into biofuel. The cell membrane is a focal point of toxicity, and understanding how it interacts with fuels and solvents is key to improving yield. Phospholipid bilayers are the core of biomembranes, and model biomembranes of defined composition provide the ideal platform for biophysical studies. To this end, glycerophospholipids characteristic …

Exploration Of Privilege And Preschool Teachers’ Demographics Associated With Teachers’ Self-Efficacy In Culturally Responsive Classroom Management, Katherine Madison

Electronic Theses and Dissertations

Every child in America is entitled to a free public education; however, racial disparities in academics and discipline continue to grow in America’s school system (Glock et al., 2019; Muñiz, 2019). These racial disparities begin in preschool, following the students throughout their school years. African American students, specifically African American males, are three times more likely than their Caucasian peers to be suspended in preschool (CRDC, 2016; Musu-Gillette et al., 2016). Suspension at an early age correlates with students entering juvenile detentions, prisons and incompletion of schooling (Love, 2014; Meek & Gilliam,2016; Muñiz, 2019). Culturally responsive teaching and classroom management …

Community College Students' Perceptions Of Sense Of Community And Instructor Presence In The Online Classroom, Marla Cartwright

Electronic Theses and Dissertations

The purpose of this non-experimental, comparative, quantitative study was to determine if there were significant differences between the perceptions of male and female community college students about the importance of sense of community (SoC) in online classes and sense of instructor presence (IP) at eight southern, public, community colleges using survey data. It was the intent of the study to determine if there were significant relationships of students’ perceptions of the presence of sense of community in online classes among factors of age, race, grade point average, cumulative credit hours, credential type, major area of study, and number of previous …

Once Upon Online: Conversations With Professional Storytellers About Adapting From In-Person To Virtual Storytelling Performance, Leticia Pizzino

Electronic Theses and Dissertations

This study examines how professional storytellers negotiated a new storytelling stage—the videoconference platform—as they pivoted their careers during mandated shutdowns due to COVID-19. An examination of the literature reveals extremely limited research involving either professional storytellers or live virtual storytelling. After interviewing five professional storytellers, I analyzed their stories through narrative inquiry. Analysis revealed that the storytellers negotiated the limitations and affordances of Zoom and adapted their storytelling to successfully connect with their audiences. Through crafting a narrative of their stories, I was able to represent their emotions, unique experiences, and abilities to adapt to the online environment. Their stories …

Music With A Racial Nexus: Culture Clash In Los Angeles Punk Rock Communities, 1976-1981, Lawson Hammock

Electronic Theses and Dissertations

The chief argument of this work rests on the idea that culture-blindness—especially White ethnic-cultural blindness—contributed as much as issues of race to the heavily documented social rift between predominantly Chicanx, Eastside punk and the mostly-White, Westside punk rock communities of Los Angeles, 1976-1981. To date, historical blame for the divide has centered on racism, including racist intent. The second area of analysis directly relates to the first in that it demonstrates the inextricable link between cultural and spatial identity formation and assignment among the various scenes. This aspect of the study evaluates the complaints of some Eastside acts who have …

Flavonol Glucosylation: A Structural Investigation Of The Flavonol Specific 3-O Glucosyltransferase Cp3gt, Aaron S. Birchfield

Electronic Theses and Dissertations

Flavonoid glycosyltransferases (GTs), enzymes integral to plant ecological responses and human pharmacology, necessitate rigorous structural elucidation to decipher their mechanistic function and substrate specificity, particularly given their role in the biotransformation of diverse pharmacological agents and natural products. This investigation delved into a comprehensive exploration of the flavonol 3-O GT from Citrus paradisi (Cp3GT), scrutinizing the impact of a c-terminal c-myc/6x histidine tag on its enzymatic activity and substrate specificity, and successfully achieving its purification to apparent homogeneity. This established a strong foundation for potential future crystallographic and other structure/function analyses. Through the strategic implementation of site-directed mutagenesis, a thrombin …

Elucidation Of The Role Of Avocado Wri1 And Wri2 And Their Ability To Affect Oil Content And Composition When Co-Expressed With Pdat1 And Dgat1, Jyoti Ranjan Behera

Electronic Theses and Dissertations

Plants synthesize and store oil, mostly as triacylglycerols (TAG), in seeds that is transcriptionally controlled by WRINKLED1 (WRI1), an APETALA2 (AP2) transcription factor. Among the four Arabidopsis WRI paralogs, WRI2 is nonfunctional, while the others are expressed in a tissue-specific manner. Additionally, two rate-limiting enzymes, diacylglycerol acyltransferase (DGAT) and phospholipid diacylglycerol acyltransferase (PDAT) catalyze the terminal step in TAG assembly and contribute to oil accumulation. Avocado (Persea americana) mesocarp, a non-seed tissue, accumulates significant amounts of TAG (~70% by dry weight) enriched with heart-healthy oleic acid. The oil accumulation stages in mesocarp coincide with the high expression of …

Women Leaders In The Oil And Gas Industry: How They Lead With Authenticity And Deal With Underrepresentation And Gender Bias In The Macro, Meso, And Micro Levels Of Society, Michelle Ann Ballard

Electronic Theses and Dissertations

This study was conducted between March 2023 and April 2023 and measured if women leaders in the oil and gas industry could lead authentically while dealing with gender biases. Women executives encounter macro-level challenges such as recruitment, retention, maintaining a career in oil and gas, feeling a sense of belonging, and gender prejudices. Women have faced organizational obstacles and challenges such as modifying their personalities, being underrepresented in their organization, unequal compensation, discriminatory stereotypes, and scarcity of resources. However, women in the industry also confront nonorganizational deterrents such as work-life conflict, harassment, and being regarded as poor advocates for their …

Alumni Giving From Online Learners: A Logistic Regression Study, Jaqlyne S. Jackson

Electronic Theses and Dissertations

Alumni giving is increasingly important to the vitality of institutions of higher education. With governmental financial support of public institutions steadily declining, understanding philanthropic giving is more important than ever. Advancement offices that serve alumni populations benefit from analyzing predictors of the behavioral phenomenon of charitable giving. It is also known that enrollment in distance education programs is on the rise in the United States. For university administrators and advancement professionals to be proactive in cultivating relationships with alumni that attended through an online modality, more research was needed to understand what motivates philanthropic giving from this alumni constituency. Using …

Obstetric Violence And Postpartum Adjustment: Exploration Of Risk And Resilience Factors, Hope O'Neill

Electronic Theses and Dissertations

Obstetric violence includes acts of abuse, coercion, or disrespect that occur during the labor process. The present study explores how obstetric violence impacts a person’s postpartum psychosocial adjustment. This study used a subset of data (N = 339) from a larger online study, which attempted to explore multiple facets of a person’s postpartum health. The psychological constructs examined are postpartum depression and postpartum anxiety. Additional constructs assessed were protective and risk factors: self-compassion and medical mistrust. Self-compassion and medical mistrust were examined by using moderation models. Additional analyses were completed using simple regression models to explore whether obstetric violence predicts …

How Is The Christian Virtue Of Detachment Epistemically Transformative?, Simon C. Summers

Electronic Theses and Dissertations

This thesis aims to understand how the Christian virtue of detachment, understood as the proper ordering of one’s desires toward God, contributes to epistemic transformation. In order to achieve this result, I rely on the work of the Trappist monk, Thomas Merton, beginning by mapping out his conception of detachment and demonstrating how it helps one to overcome competing desires and find their true self. Following this, I offer an account of the type of epistemic transformation required to access some theistic knowledge in order to connect Merton’s views on the topic with those of modern-day philosophers and show the …

Understanding Burnout Through The Lens Of Teachers During The Covid-19 Pandemic: A Phenomenological Study, Rita Marie Garcia

Electronic Theses and Dissertations

Abstract As teachers make their way back to the classroom for the 2023–2024 school year, teacher burnout is manifesting itself mentally, physically, and emotionally. In March 2020, the World Health Organization declared the COVID-19 epidemic a global pandemic, which forced unwanted change in many organizations, especially education (Pressley, 2021). In the state of emergency schools were forced to close and new teaching methods were erected abruptly. The Robert Wood Johnson Foundation describes the professions of teaching as one of the most stressful occupations in the United States. Although stress is inevitable, the COVID-19 pandemic has created new stressors and demands …

Formalizing The Faustian Bargain Within The Healthcare Domain: An End-Of-Life Approach., Rachel Appel

Electronic Theses and Dissertations

A Faustian Bargain refers to an individual making a “deal with the devil,” exchanging something moral or sacred for an unattainable earthly good. The Faustian Bargain has been used to describe a ubiquitous social dilemma inherent to human civilization: exchanging individual liberty for public goods (e.g., security) provided by societal leaders and governments (Ostrom, 1980). Research on Faustian Bargains often examines tradeoffs between outcome utility (i.e., value derived from the outcomes of a decision) and procedural utility (i.e., value derived from being involved in the decision process (e.g., Frey et al., 2004). Much of the research on Faustian Bargains has …

Organizational Change In Saudi Healthcare Settings: Evaluating Organizational And Individual Readiness For Change, And The Mediating Role Of Readiness For Change Between Management Support And Commitment To Change., Sultan Alsaif

Electronic Theses and Dissertations

BACKGROUND: To respond to the constantly changing environment and developments of healthcare, leaders of healthcare organizations have been trying to introduce and implement transformations that allow their organizations to be able to operate effectively and efficiently to meet the shifts in healthcare demand and to deal with new patterns of health issues, comply with the new policies, and to enhance their present in the market. Thus, it is important for managers to determine the level of readiness for implementing organizational changes from to perspectives. These perspectives include organizational readiness for change and individual readiness for change. METHOD: This first manuscript …

What Discrimination? Christian Microaggression Rhetoric Against Nontheists, Nicole Dolfi Hall

Electronic Theses and Dissertations

Theories of microaggressions have been used in the last decade as a framework for studying subtle forms of discrimination against racial minorities and other marginalized groups. However, there is a dearth of research on the scope and types of microaggressions nontheists face. This qualitative study examines microaggressions against nontheists by interviewing 16 religious Christians on their experiences and opinions of the discrimination Christians and nontheists face. The narratives were analyzed for thematic patterns between the rhetoric used and the type of microaggressions employed. The study revealed nine categories of microaggressions. The findings also showed how experiences of discrimination, political viewpoint, …

The Interplay Between Lung Adenocarcinoma, Nutrient Availability, And The Microbiome., Alexis A. Vega

Electronic Theses and Dissertations

Lung cancer is one of the most common forms of cancer diagnosed, and has the highest lethality rate across all other forms of cancer in the U.S. While current therapeutic options include chemo-, immuno-, and radio-therapy, the benefits of caloric or nutrient restriction on cancer cells has also been investigated. Due to the many biological functions associated with methionine, many have proposed a methionine restricted diet would lead to favorable outcomes when combating cancer. Although our cells are incapable of synthesizing methionine, the bacteria found in our microbiome can. Furthermore, recent discoveries suggest an independent microbiome found within tumors that …

Investigating The Effects Of Subchronic Dietary Zinc Supplementation On Diet-Induced Metabolic Dysfunction-Associated Steatotic Liver Disease., Oluwanifemi Esther Bolatimi

Electronic Theses and Dissertations

Zinc deficiency is associated with metabolic dysfunction-associated steatotic liver disease (MASLD). Previous studies show zinc supplementation improves steatosis, but its therapeutic effects in established MASLD remain unclear. We developed a model to characterize the effects of zinc supplementation on high-fat diet (HFD) induced MASLD and hypothesized established MASLD would be attenuated. Mice were fed control diet or HFD for 12 weeks and then grouped into normal or zinc-supplemented diets for 8 additional weeks. At euthanasia, plasma and liver tissues were collected for phenotypic analysis. Twelve weeks of HFD altered glucose clearance and body composition. Eight weeks of subsequent zinc supplementation …

The Impact Of Environmental Pollutants On Alcohol-Associated Liver Disease Pathogenesis., Tyler Charles Gripshover

Electronic Theses and Dissertations

Steatotic liver disease is brought upon by differing etiologies and its global prevalence is expected to keep increasing over the next several decades. Particularly, alcohol-associated liver disease (ALD) manifests from excessive alcohol consumption where the liver retains lipids and progresses with metabolic dysfunction. Previous studies have demonstrated that environmental pollutants, polychlorinated biphenyls (PCBs) promote metabolic dysfunction associated steatotic liver disease (MASLD), in diet-induced obesity models. However, it is unknown if environmental pollutants may also promote ALD pathogenesis. Thus, this dissertation focuses on characterizing the effects of PCB126 in a rodent ALD model followed by multiple ‘Omics approaches to identify mechanisms …

Communicating In Crisis: Rhetorical (De)Stabilization During The Covid-19 Pandemic., Brittany Nicole Smart

Electronic Theses and Dissertations

This project explores the role of rhetoric in crisis—how rhetoric can contribute to both the stabilization and destabilization of a worldwide health emergency. Specifically, I utilize the COVID-19 pandemic as a case study to investigate how institutional rhetorics exacerbated the ongoing burnout epidemic amongst healthcare workers. Through a feminist, materialist take on institutional ethnography (Fullagar & Pavlidis, 2021; Griffith & Smith, 2014), I show how, while institutions like the CDC were under pressure to contain the spread of the virus, in the chaos of communicating safety regulations to healthcare professionals, they inadvertently subverted clinician autonomy and expertise by “coordinating” (LaFrance, …

Using A Toxic Aging Coin To Assess Hexavalent Chromium-Induced Neurotoxicity., Samuel Thomas Vielee

Electronic Theses and Dissertations

We are facing an aging crisis, with 20% of the U.S. population projected to be geriatric (65+) by 2030 and live another 40+ years. Age-related diseases accompany a growing geriatric population, emphasizing the need to understand their etiology. Environmental pollutants compound this crisis by: 1) geriatrics are more susceptible, exacerbating age-related diseases and comorbidities, and 2) they accelerate biological aging, inducing age-related diseases at younger ages. We address this crisis using a ‘toxic aging coin’ approach; heads examines how age impacts toxicity, tails examines how chemicals accelerate aging. This thesis applies the heads side for Cr(VI)-induced neurotoxicity across ages. We …

A Study Of Group Effectiveness In A Virtual Reality Environment., Dalton Clay Sparks

Electronic Theses and Dissertations

Virtual Reality (VR) technology has revolutionized the study of team effectiveness, yet many studies in this domain lack a comprehensive approach, focusing narrowly on performance metrics. This thesis investigates VR team effectiveness by integrating traditional Team Effectiveness frameworks with VR-specific dynamics. Utilizing Data Envelopment Analysis (DEA) and Group Style Inventory (GSI), this study assesses teams within a VR environment, specifically a multiplayer submarine simulation game, IronWolfVR. Fifteen groups, totaling 45 participants, were observed for their collaborative gameplay, incorporating physiological measures (Heart Rate and Electrodermal Activity), team coordination dynamics, and various survey-based assessments (demographics, System Usability Scale, Simulator Sickness Questionnaire, and …

From "Smart Talk" To "Living Well": Commonplaces And Their Role In Narratives Of Rare Disease., Caitlin E. Ray

Electronic Theses and Dissertations

As healthcare becomes more complex, automated, and bureaucratic, patients often suffer from a lack of resources, agency, and visibility when seeking medical care. Rhetoric and Composition, specifically the subfield of Rhetoric of Health and Medicine (RHM), is interested in studying and intervening into such issues. One way to challenge our current understanding of healthcare is to consider how the rare disease patient experience reveals the gaps, limitations, and assumptions of illness and health. I argue here that through rare diseases, rhetoricians of health and medicine can better understand the representation, advocacy, and patient experience within healthcare, and potentially lead to …

Computational Investigations Of Bond Breaking Processes Using Dft And Td-Dft Approaches., Saurav Parmar

Electronic Theses and Dissertations

The efficient application of DFT and TD-DFT has been harnessed to study bond-breaking processes in some molecules which play a prominent role in enzymatic reactions. The first application includes Radical S-adenosyl methionine (SAM) enzymes which are fundamentally important sources of organic radicals to initiate diverse radical reactions. Recently a bio-organometallic intermediate (Ω) that contains an Fe‒C bond has been characterized and shown to be a common feature of radical SAM enzymes. The strength of Fe‒C bond in Ω has been computed using broken-symmetry density functional theory (BS‒DFT). Additionally, Fe‒C bond dissociation energy (BDE) in Ω has been compared to that …

Representing The Mixed Plate: Involving Descendant Communities And Kānaka Maoli In Hawai’I Plantation Museums, Amanda Ku’Ualohalanileimakamae Lane

Electronic Theses and Dissertations

This thesis explores the ways that the involvement of diverse stakeholders at Hawai’i plantation museums affects representations of Hawai’i’s plantation history. Plantations in Hawai’i had a direct colonizing effect on Kānaka Maoli (Native Hawaiians), displacing them from their lands, replacing them with immigrant laborers, and putting into motion the chain of events that led to Hawai’i’s annexation in 1898. The current-day population in Hawai’i continues to reflect these significant changes in the society and culture of the islands. Hawai’i’s plantation museums traverse topics of labor, immigration, indentured servitude, and colonization. Simultaneously, these museums advance stories of perseverance, celebration, and multiculturalism. …

Likeness In Utopia: Situation And Metaphor From Thomas More To Edward Bellamy, Sage Rachmiel Bard Gilbert

Electronic Theses and Dissertations

As a literary genre, utopia is notably didactic. It seeks to teach desire and to educate hope. As such, utopia provides a unique site to examine the way metaphor and imagination enable one to be convinced, and the way those same elements facilitate misunderstanding. Following the theorization of Ernst Bloch, the goal of critiquing these literary utopias is not to reject hope but, rather, to educate our own daydreams, to learn and move forward. These chapters examine didacticism and the development of colonial metonymy in Thomas More’s Utopia, the way metaphor operates through time in Edward Bellamy’s Looking Backward: …

Frequency Stability Constrained Grid Operation With High Penetration Of Renewables, Ningchao Gao

Electronic Theses and Dissertations

Achieving carbon neutrality necessitates a significant integration of renewable energy into power systems. However, the swift implementation of fluctuating renewable energy (VRE) sources such as solar photovoltaics (PV) exacerbates real time power imbalances due to the randomness and uncertainty associated with VRE power generation. Further, the declining reliance on conventional synchronous generators (SGs) for system inertia presents considerable challenges in maintaining frequency stability, especially following disturbances.

Two possible solutions are proposed to address the first issue. Firstly, a speedy real time generation dispatch can be scheduled to allocate generation resources within short time intervals to effectively respond to changes in …

The Use Of Regularization To Detect Racial Inequities In Pay Equity Studies: An Empirical Study And Reflections On Regulation Methods, Christopher M. Peña

Electronic Theses and Dissertations

Since the late 1970s, multiple linear regression has been the preferred method for identifying discrimination in pay. An empirical study on this topic was conducted using quantitative critical methods. A literature review first examined conflicting views on using multiple linear regression in pay equity studies. The review found that multiple linear regression is used so prevalently in pay equity studies because the courts and practitioners have widely accepted it and because of its simplicity and ability to parse multiple sources of variance simultaneously. Commentaries in the literature cautioned about errors in model specification, the use of tainted variables, and the …

Safest Kid (A Sexual Assault Framework In Education To Support Trauma In Kids With Intellectual Disability): Delphi Study Development Of A Model And Utilization, Ashley M. Hudson

Electronic Theses and Dissertations

There is a grave need for additions to the school psychologist’s toolbox to support students with intellectual disability (ID) and sexual trauma. These children are especially vulnerable to adverse life experiences overall and are at a particularly high risk of experiencing sexual abuse and resulting trauma. Children with ID are less likely to have their trauma symptoms identified by those around them, as symptoms do not always present in the same way as their neurotypical peers and trauma symptoms are more likely to be grouped into the rest of their disability through diagnostic overshadowing. Additionally, individuals with ID are at …

Putting It Gently: The Other Side Of A Peace Corps Volunteer Experience, Nicole Jacobson

Electronic Theses and Dissertations

The Peace Corps is a transformative experience for many young Americans. The majority of volunteers have worthwhile services they remember fondly; however, these experiences are not always positive. The Peace Corps is a Cold War initiative that uses soft power techniques to further American exceptionalism, capitalism, and the modernization theory of development. The goal of this project was to understand what factors led to the negative experiences of Peace Corps Volunteers. I conducted an ethnography that focused on online communities, semi-structured interviews, Peace Corps memoirs, and news articles to understand Peace Corps Volunteers’ experiences and the organizational culture where they …

Encounter-Oriented Subjectivity In Levinas And Barth: Conditioned Freedom In Resistance To Ethnonationalism, Michael Laminack

Electronic Theses and Dissertations

This dissertation examines philosophical and political subjectivity, or how an individual knows the world and relates to society, through a comparative analysis of the phenomenologist Emmanuel Levinas and the Christian theologian Karl Barth. Levinas and Barth share a deep structure of “encounter-oriented subjectivity,” meaning subjectivity oriented by encounter with the Other that calls into question the subject’s freedom and, in this same moment, calls the subject to concrete response. Encounter-oriented subjectivity is critical of the “unconditioned freedom” that orients liberal subjectivity and offers an alternative sense of a “passively conditioned” freedom, or a freedom always already called into question by …