Digital Commons Network^™

The Role Of The Toll-Like Receptor Pathway In Susceptibility To Inflammatory Bowel Disease., Nigel Crawford 1974-

Electronic Theses and Dissertations

Inflammatory bowel disease (IBD) is a chronic autoimmune disorder that is subdivided into Crohn's disease (CD), ulcerative colitis (UC), and indeterminate colitis (IC). Epidemiological studies have proven that genetic variation increases susceptibility to IBD, with multiple abnormal genes combined with environmental factors being responsible for disease development. Characterization of these susceptibility genes remains of critical importance to improving understanding of IBD pathogenesis. The overall aim of this study is to discover new IBD susceptibility genes. The initial approach was to study a number of previously described IBD susceptibility loci through characterization of peak LOD score short tandem repeat markers using …

The Transcriptional Repressor Protein Growth Factor Independence-1b In T Lymphopoiesis., Loretta L. Doan

Electronic Theses and Dissertations

T lymphopoiesis has been an intense focus of immunological research since the discovery of the major histocompatibility complex (MHC) and T cell mediated transplant rejection. Additionally, researchers have long appreciated the role of T cells, and their development, in autoimmune disorders. Interest in this field has increased upon the realization that many leukemic oncoproteins are the very factors that control normal T cell development. Thus, the transcriptional networks that drive the function and development of T lymphocytes are closely linked with disease states.Growth Factor Independence 1 (GFI1) and GFI1B are two very similar transcriptional repressor oncoproteins that are encoded by …

The Cost Of Wisdom : A Comparative Theoretical Analysis Of Research Ethics In The United States, Germany And Hungary., Steven A. Drewry

Electronic Theses and Dissertations

This dissertation is a comparative theoretical analysis of human rights, research ethics and human subjects protection. The history of human rights is a long one, but for the sake of immediacy and focus, the author will limit the discussion to events pertaining to the last century and the beginning of a new one. Through a process of sociocultural review of significant human rights and scientific events pertinent to research involving human subjects, this work will examine the evolution of research ethics in the United States, Germany and Hungary. These nations are not intended to be representative of international research ethics …

Religion And Crime: A Study Of Inmates In State And Federal Prisons In The United States., Dhanalakshmi Thirumalai

Electronic Theses and Dissertations

Persistent high levels of crime in this country have provided support for the need for public programs that help in reducing crime rates through effective interventions. Spirituality and religion are considered to play a major part in determining an individual's attitude towards committing a crime. This study analyzed if inmates in State and Federal prisons in the US are involved in prison religious activities and personal prayers in order to determine if criminality (measured by offense type) of inmates affects their participation in religious activities. Based on logistic regression analysis, gender, race, and marital status of inmates were found to …

The Visual Perception Of Distance In Action Space., Bing Wu 1973-

Electronic Theses and Dissertations

This work examines our perception of distance within "action space" (about 2m ~ 30m), an ability that is important for various actions. Two general problems are addressed: what information can be used to judge distance accurately and how is it processed? The dissertation is in two parts. The first part considers the "what" question. Subjects' distance judgment was examined in real, altered and virtual environments by using perceptual tasks or actions to assess the role of a variety of intrinsic and environmental depth cues. The findings show that the perception of angular declination, or height in the visual field, is …

Population Size And Movements Of Spotted Salamanders At South Holston Dam, Sullivan County, Tennessee., Eric Alexander Smith

Electronic Theses and Dissertations

In the Southern Appalachians, there are growing concerns about Spotted Salamanders (Ambystoma maculatum) due to habitat destruction and the uncertain future of their forested riparian habitat. In this study, I establish baseline data of a population of Spotted Salamanders so future monitoring of this population can be performed. The study site is located in northeastern Tennessee, just east of the city of Bristol. It is a vernal pond at the Osceola Island Recreation Area one mile below the South Holston Dam on Holston View Dam road. A drift fence was established completely around the perimeter of the vernal …

The Effect Of Process Parameters On Laser-Deposited Ti-6a1-4v., Troy Austin Davis 1981-

Electronic Theses and Dissertations

The purpose of this research is to understand how the process parameters surrounding the Direct Metal Deposition (DMD) process affect the properties of the deposition. The powder used in this research is pre-alloyed titanium powder (Ti-6Al-4V), containing 6% wt. aluminum and 4% wt. vanadium. A few process parameters involved with the DMD include the laser power, traverse speed, powder flow rate, and gas flow rates. Due to the detrimental effect of oxygen on titanium, oxygen content is one of the main deposition properties of significance involved with this research. In addition to the oxygen content, the powder efficiency, build rate, …

Nutritional Content Of Supplemental Food Baskets Distributed By Charitable Agencies In Northeast Tennessee., Lillian Khalili Aliata

Electronic Theses and Dissertations

The purpose of this study was to determine if the nutritional needs of high-risk community members are met by supplemental food baskets distributed by charitable groups in northeast Tennessee. Hunger is a real problem among the area's elderly population, as 14.3% of the populations live at or below the poverty level. Data were collected using observation interviews at the food bank. A nutrient analysis tool was used to analyze the nutrient content of food items in a standard food basket. The findings were compared to the recommended dietary allowance for the elderly population. The supplemental food basket was adequate in …

The Relationship Between The Use Of Developmentally Appropriate Practice And The Inclusion Of Product-Producing Art Activities In Infant Programs., April D. Moore

Electronic Theses and Dissertations

Product-producing art activities (PPAA) produce visual products, disregarding developmentally appropriate practice (DAP) and the creative learning experience. Infant programs in Northeast TN were examined to determine if as PPAA levels increased DAP levels decreased, which was unfounded: t (2) = 1.80, n.s. In fact, significant differences were found between programs with high PPAA levels and DAP as indicated by scores on the Infant/Toddler Environment Rating Scale (Harms, Cryer, & Clifford, 1990) sub-scales: Furnishings and Displays, t (2) = 7.59, p < .05; Listening and Talking, t (2) = 6.71, p < .05; and Learning Activities, t (2) = 7.29, p < .05. Caregivers' main reason for including PPAA was: positive sensory experience; main reason for exclusion: infant/teacher relationship is more important.

Power And Perfection In Karen Finley's The Constant State Of Desire: Creating A New Discourse., Melissa D. Greenwood

Electronic Theses and Dissertations

Karen Finley's The Constant State of Desire merits attention because it acknowledges modern language's inability to represent the suffering of victims and creates awareness of our personal involvement in constructing gendered identities. Finley expresses her abhorrence of the desire for power and perfection by asserting that power is secured in American culture through physical and economic domination. In addition, the pursuit of perfection is engrained in one's psyche through media images and habituated behaviors. Finley does not offer a new language through which to communicate suffering, but she draws the reader's attention to the inadequacies of psychological and cultural rhetoric, …

Bridging The Gap Between Medical Science And Communication: An Interpretive Analysis Of Messages Portrayed On Endometriosis Websites., Lakesha Nichole Anderson

Electronic Theses and Dissertations

This study examined women's health messages found on ten endometriosis websites. Qualitative research methods were used to investigate messages available via Internet media about causes and treatments of endometriosis, particularly as they relate to the suggestion that hysterectomy and pregnancy are effective treatments. Messages about infertility, physician-patient communication, and accessibility were also examined. Findings indicate that the websites provided similar messages regarding the symptoms, causes, and treatments of endometriosis; results pertaining to infertility were mixed. Little information was available on methods of improving physician-patient communication. Most websites provided additional low-cost information while requiring minimal technological competency or additional software of …

Epiphanies Of The Principalship: A Study Of Passages In Educational Administration., Donna J. Raines

Electronic Theses and Dissertations

This scrapbook of biographical sketches serves as an exhibition of portraits in the elementary school principalship through experiencing a gallery of leadership interviews. Perspectives have been framed in compositions that represent the practical realist, the idealist, and at times even a surrealistic perspective of the elementary principal. Critical incidents were shared from individual participants representing catalysts for momentous change; themes of personal realizations, barriers to effectiveness, and challenges in the career of an administrator also contributed as dominant elements of the final work.

A qualitative research method employing interviews explored the variation of colorful experiences that 23 elementary administrators encountered …

Wrestling With Father Shakespeare: Contemporary Revisions Of King Lear And The Tempest., Erin Melinda Denise Presley

Electronic Theses and Dissertations

In Shakespeare’s The Tempest and King Lear, the relationship between the father and his children affects the progression and outcome of events. Goneril and Regan oppose Lear after Cordelia’s untimely rebellion and disownment. In The Tempest, Caliban desires to overthrow Prospero for freedom. Similarly, the appropriative offspring also exhibit rebellious “children” challenging authority. In Jane Smiley’s revision of King Lear and Aimé Césaire’s rewriting of The Tempest, defiance renders the children fatherless. In Disney’s The Little Mermaid, Ariel initially disregards her father but ultimately accepts his rule. In Gloria Naylor’s Mama Day, the text itself becomes an orphan as the …

Determination Of Lactose By Reversed-Phase High Performance Liquid Chromatography., Danessa Leann Sexton

Electronic Theses and Dissertations

Lactose is the common disaccharide found in dairy products. It can, however, be a source of discomfort for those whom are lactose intolerant. Therefore, it is necessary to accurately, reliably, and efficiently determine the lactose content of foods. The goal of this research was to develop a reversed-phase HPLC method with UV detection for the determination of lactose. Aminobenzoic acid was used to prepare a derivative, making detection possible in the 300-360 nm region. The stationary phase is a C8 column, with 85% methanol 15% water mixture at pH 5 as the mobile phase. A linear response of peak area …

A Church Crisis: A Rhetorical Criticism Of Communication Arguments From Proponents Of Both Sides Of The Homosexual Movement In The Christian Church, Garland Brent Wallace

Electronic Theses and Dissertations

The increased levels of homosexual discussion in mainstream media culture have aroused opinion diversity in both secular and religious circles. The case of Bishop Gene Robinson has brought the issue of homosexuality in the priesthood to the forefront. However, the main issue does not rest on the issue of homosexuality but rather on the inspiration, validity, and authority of the Bible as scripture. After the evaluation of two pieces of rhetoric, including an interview given by Bishop Gene Robinson on a local radio broadcast and a speech given by B. L. Mauser concerning homosexuality as sin, Kenneth Burke's pentad will …

The Effect Of Tourist Theft On Future Travel Decisions, Judith Lynn Holcomb

Electronic Theses and Dissertations

Past research has shown that theft is a prevalent crime against tourists. This study's purpose was to determine the effects of past incidents of personal theft on tourists' future decisions to travel by analyzing and comparing those who have experienced personal theft to those who heard of such incidents through personal accounts of friends or family. The findings, which were taken from a survey of 215 respondents, revealed that that experiencing personal theft, or knowing of someone who has, is not a deterrent for visiting a destination where the theft occurred. Results also showed that one aspect of theft that …

Molecular Typing Of Mycobacterial Isolates Cultured From The Tissue Of Inflammatory Bowel Disease (Crohn's Disease) Patients, Leanne M. Adams

Electronic Theses and Dissertations

The role of Mycobacterium avium subsp paratuberculosis (MAP) in the etiology and pathogenesis of inflammatory bowel disease (IBD) including Crohn's Disease (CD), has been investigated. The fastidious characteristics and cross reactivity of MAP with other members in Mycobacteria have produced significant challenges in their detection and identification. In this two year pilot study, an array of three PCR molecular assays based on the detection of sequences from the16S rRNA, IS1245, and IS900 genes, belonging to members of the MAC, have been developed and optimized into a common protocol to be used as a rapid and accurate diagnostic tool regarding M. …

Interactions Between The Nudibranch Okenia Zoobotryon And Its Bryozoan, Nicole Robinson

Electronic Theses and Dissertations

In the Indian River Lagoon, the nudibranch Okenia zoobotryon and its egg masses are found attached to the bryozoan Zoobotryon verticillatum throughout the year. Okenia zoobotryon is thought to live, feed, and reproduce exclusively on Z. verticillatum, which would make this a very specialized predator-prey interaction. The primary goal of my study was to document certain aspects of the ecological and chemical relationships between Z. verticillatum and O. zoobotryon. Specifically, I wanted to understand the cues used by the nudibranch to locate and remain on Z. verticillatum. Population surveys on Z. verticillatum, as well as other potential hosts, were performed. …

Extracullular Atp Regulates Il-1beta Release From Microglial Cells Via Purinergic Receptor After In Vitro Trauma, Chengya Liang

Electronic Theses and Dissertations

Traumatic brain injury (TBI) induces a state of microglialactivation, which includes upregulation of macrophage functions and release inflammatory mediators such as certain inflammatory cytokines. Current literature suggests that interleukin-1Beta is an important cytokine mediator, which is dramatically increased after brain injury. Previous studies indicate that ATP is released by traumatically injured astrocytes and serves as a cell-to-cell mediator through purinergic receptors after in vitro injury. However, the mechanism of interleukin-1Beta release after traumatic brain injury remains poorly defined and is difficult to study using in vivo models. Using an in vitro model for traumatic brain injury (cell strain or stretch), …

The Impact Of Traditional Shona Beliefs On Hiv/Aids Intervention Work In Zimbabwe, Brian Goercke

Electronic Theses and Dissertations

This study examined the relationship between secondary students in the rural and peri-urban areas of Mashonaland in Zimbabwe and their tendencies to agree or disagree with traditional Shona attitudes, beliefs and practices as they related to the HIV/AIDS pandemic. A convenience, cluster sample was used to identify the 482 students who participated in this study. A questionnaire composed of demographics, hypothetical and open-ended questions was administered to these students within the regular classrooms of their respective schools.

A notable finding in this study was that traditional Shona beliefs appear to be stronger in the rural areas. However, the students surveyed …

The Importance Of Cmos Sensor Surface Topography In The Attachment, Proliferation, And Differentiation Of Human Adult Mesenchymal Stem Cells, Christa Y. Heyward

Electronic Theses and Dissertations

Osteoblasts and osteoblast precursor cells (mesenchymal stem cells) respond to the surface topography of metal implants by aligning along surface features in order to minimize shear stress. While several previous studies have investigated the morphology, attachment and proliferation of both osteoblasts and mesenchymal stem cells along grooved surfaces, the current study investigates the cellular response to depressions and peaks in surface topography, as well as surfaces that contain alternating combinations of depressions and peaks. The Complementary Metal Oxide Semiconductor (CMOS) sensor is a transducer fabricated from silicon and sputter coated with titanium. The CMOS sensor is designed to utilize piezoresistive …

Analysis Of Specific Cis-Acting Dna Sequences Of The Himar1 Mariner Transposon, Beth Berta

Electronic Theses and Dissertations

Himar1, found in the horn fly Haematobia irritans, belongs to the mariner family of transposable elements. Mariners are a diverse and widespread family of eukaryotic transposons that transpose via a cut-and-paste mechanism. Mariner transposition not only requires the transposase, but also specific cis-acting DNA sequences known as inverted terminal repeats (ITRs). Very little is known about the fine structure of mariner ITRs, particularly which bases are important for transposition. A mariner family ITR sequence logo representing 20 different mariners from six different subfamilies suggests that more conserved regions of the logo are required for transposase binding while the more variable …

Critical Digital Infrastructure Protection: An Investigatoin Into The Intergovernmental Activities Of Information Technology Directors In Florida Counties, Joah Nicole Devenny

Electronic Theses and Dissertations

As cyber attacks become more sophisticated, the risk to all networked computer systems increases. Whether public or private, whether federal, state, or local, the threat is equally real. Consequently, local governments must respond accordingly to understand the threats, take measures to protect themselves, and determine how to respond in the event of a system breach. Additionally, since cyber criminals do not respect geographic or administrative boundaries, local leaders must be prepared to instantly interact with other governments, agencies, and departments to suppress an attack. Guided by the theory of intergovernmental management (IGM), this exploratory research investigated how Information Technology (IT) …

Neural Networks Satisfying Stone-Weiestrass Theorem And Approximating, Pinal Thakkar

Electronic Theses and Dissertations

Neural networks are an attempt to build computer networks called artificial neurons, which imitate the activities of the human brain. Its origin dates back to 1943 when neurophysiologist Warren Me Cello and logician Walter Pits produced the first artificial neuron. Since then there has been tremendous development of neural networks and their applications to pattern and optical character recognition, speech processing, time series prediction, image processing and scattered data approximation. Since it has been shown that neural nets can approximate all but pathological functions, Neil Cotter considered neural network architecture based on Stone-Weierstrass Theorem. Using exponential functions, polynomials, rational functions …

Vitellogenin Induction As A Biomarker For Environmental Estrogens In Xenopus Laevis, Jamie Skoloda

Electronic Theses and Dissertations

In oviparous vertebrates, estrogen stimulates the liver to produce vitellogenin. Vitellogenin induction is a useful biomarker for exposure to endocrine disruptors, specifically environmental estrogens. The objective of this project was to design in vivo bioassays for environmental estrogens based on induction of vitellogenin in male Xenopus laevis. Frogs were exposed to ethinyl-estradiol by injection or immersion for different times and doses, than vitellogenin induction was assessed. An ELISA was developed to measure serum vitellogenin. Additionally, a vitellogenin rt-PCR assay was developed using species-specific vitellogenin primers, to detect mRNA induction. It was established that the induction of serum vitellogenin and hepatic …

Identification Of Protein Interaction Between The Drosophila Runx1 Transcription Factor Lozenge And Ets-1 Factor Pointed Using Site Directed Mutagenesis And Yeast Two-Hybrid Analysis, Shalini Singh

Electronic Theses and Dissertations

Many classes of transcriptional regulatory proteins are known to function in both cell proliferation and differentiation. Runx1 proteins one such family of transcription factors, plays critical roles in hematopoiesis, osteogenesis and leukemogenesis and act as promoter organizers that cooperate with other transcription factors such as Ets-1 in the regulation of gene activation or repression. Genes that are regulated by the Runx1-Ets1 complex, frequently have multiple, adjacent consensus binding sites in their promoters. I have investigated a similar interaction in developing fly eye. Lozenge (DmRunx1) and Pointed P2 (DmEts-1) cooperate to upregulate expression of prospero, which has multiple Lz and Ets …

Survival Of Mycobacterium Avium Subspecies Paratuberculosis In The Pol, John Rumsey

Electronic Theses and Dissertations

Mycobacterium avium subspecies paratuberculosis (map) is an intracellular pathogen that is known to parasitize macrophages and monocytes. Map infiltrates gastrointestinal tract host tissue where it is the known etiological agent of johne's disease in ruminants and implicated in the etiology of crohn's disease in humans. Map's ability to survive within macrophages enables it to disseminate throughout the rest of the host, possibly infecting other circulating blood leukocytes. In this study, the survival and fate of map strain atcc 43015 (human isolate) following phagocytosis was determined using in vitro murine macrophage cell line j774a.1 and polymorphonuclear cells (pmnc's) from five crohn's …

The Ecology Of Drift Algae In The Indian River Lagoon, Florida, Julie Lynnae Liss

Electronic Theses and Dissertations

To gain an understanding of the ecology of drift algae in the Indian River Lagoon system along the east coast of central Florida, four questions were addressed: 1) What is the composition and rate of accumulation of drift? 2) How much movement and turnover occurs within drift accumulations? 3) Do growth rates differ for drift versus attached algae? 4) Is there a difference in photosynthetic performance in drift versus attached algal species? Manipulative field and laboratory experiments were conducted to address these questions with the green macroalga Codium decorticatum and the red macroalga Gracilaria tikvahiae. Changes in pigment concentration and …

Hydrogen Sulfide Flux Measurements And Dispersion Modeling From Constr, Sangho Eun

Electronic Theses and Dissertations

Odor problems are a common complaint from residents living near landfills. Many compounds can cause malodorous conditions. However, hydrogen sulfide (h2s) has been identified as a principal odorous component from construction and demolition (c&d)debris landfills. Although several studies have reported the ambient concentrations of h2s near c&d landfills, few studies have quantified emission rates of h2s. The most widely used and proven technique for measuring gas emission rates from landfills is the flux chamber method. Typically the flux chamber is a cylindrical enclosure device with a spherical top which limits the gas emission area. Pure zero grade air is introduced …

Association Discovery In Lung Cancer Microarray Data, Kristin E. Davidson

Electronic Theses and Dissertations

No abstract provided.