Digital Commons Network^™

Examining The Effects Of Post-Traumatic Self-Blame And Shame On Response To Laboratory-Induced Pain, Adi Rosenthal

Electronic Theses and Dissertations

Extant research has made clear the link between self-criticism and engagement in non-suicidal self-injury (NSSI). As posited by the Defective Self Model of NSSI, individuals who experience feelings of self-hatred may perceive pain as deserved and engage in NSSI (Burke et al., 2021). Individuals exposed to interpersonal trauma are at higher risk of engaging in NSSI, and also frequently experience trauma-related self criticism, namely shame and self-blame. More work is needed; however, to understand how self-critical post-traumatic cognitions contribute to risk for NSSI engagement for interpersonal trauma survivors. The current study uses a combination of lab-induced pain and self-report measures …

Unraveling The Role Of Hid-1: Insights Into Sgii Regulation, Breanna L. Sellers

Electronic Theses and Dissertations

The regulated secretory pathway is important for the secretion of hormones and neuropeptides from large dense core vesicles (LDCVs). Dysregulation of the biogenesis of LDCVs can result in metabolic and neurological diseases. Recent studies have suggested that the peripheral membrane protein HID-1 plays a role in the sorting and storage of granins which contribute to LDCV biogenesis. To further understand how HID-1 influences the regulation of granins, we investigated potential HID-1 interactors. We determined that while HID-1 is likely functioning in the same pathway as USP25, a deubiquitinating enzyme (DUB), the cause of reduced cellular SgII levels is likely not …

Colorado's Multilingual Learners And The Read Act: A Critical Discourse Analysis, Brooke K. Sassi

Electronic Theses and Dissertations

This dissertation critically examines the Colorado Reading to Ensure Academic Development Act (READ Act) through the lens of Critical Discourse Analysis, focusing on the discursive construction of the Act and its impact on educational opportunities for multilingual learners. The study explores the role of power and knowledge in shaping the READ Act and addresses the ideological tensions and power imbalances that arise from a policy primarily designed for monolingual, English-speaking students. Drawing on personal experiences and extensive research, this analysis highlights the shortcomings of a phonics-heavy approach mandated by the READ Act, particularly for multilingual learners who require a more …

A Force To Be Reckoned With: Testimonios From Latina/E Senior Leaders Across Psychology Contexts, Sandra Bertram Grant

Electronic Theses and Dissertations

Latinas currently make up 76% of overall Latino/a/e participation in psychology (American Psychological Association, 2022); however, the numbers of Latinas in top echelon leadership positions are low (National Center for Education Statistics, 2021, 2022). Notedly, the information on the current state of Latine representation in the field of psychology is even more limited. The purpose of this qualitative study is to explore the testimonios of eight Latina/es regarding their professional development, decision making process, pursuit, and persistence in leadership positions, particularly within the field of psychology. This study is guided by Testimonio (Huber, 2009) as the methodological approach and analysis, …

A Phenomenological Examination Of The Perception Of Psychopathological Misdiagnosis In Gifted Men, Tina Glass

Electronic Theses and Dissertations

While the exact prevalence of psychopathological misdiagnosis in gifted children is unknown, it is an occurrence within the gifted population (Amend & Beljan, 2009; Batstra et al., 2012; Bishop & Rinn, 2019; Bishop & Rinn, 2020; Coon, 2014; Webb et al., 2016). However, there is a gap in the literature related to psychopathological misdiagnosis specific to gifted males. (Desmet & Pereira, 2021; Ecklund et al., 2014; Eren et al., 2018; Neu & Weinfeld, 2007; OECD, 2015; Preckel et al., 2015; Watts, 2022). This transcendental phenomenological study leveraged Bronfenbrenner’s Bioecological Model for Human Development (Bronfenbrenner & Morris, 2006) as a theoretical …

Baby Dust: A Narrative Analysis Of Infertility Journeys On Youtube, Patricia Carrillo

Electronic Theses and Dissertations

Infertility is a distressing experience for those diagnosed and often exacerbated by engagement with biomedical treatments. This thesis is an exploration of infertility “journeys” posted on YouTube. Through a narrative analysis of 8 YouTube channels, several key themes were identified, specifically embodiment of the infertility label, barriers to treatment, and agency. This work uses the mindful body framework to look at how the individual body, social body, and body politic shape and reinforce these themes in the context of biomedical infertility treatment. These findings suggest infertility is a dynamic and distressing experience but by engaging in the narrative process, through …

Sequence Specificity Of Non-Structural Protein 15 From Sars-Cov-2, John Vincent Mcguire

Electronic Theses and Dissertations

The SARS-CoV-2 virus was recently able to cause a global pandemic, largely due to the asymptomatic period that occurs during initial infection. During this period, patients are infectious but do not exhibit symptoms, resulting in the unintentional spread of the virus. This occurs because the virus effectively avoids detection by the immune system during the early stages of infection, and as symptoms are the result of immune system activation, they never manifest. Non-structural Protein 15 (Nsp15) is one of the tools the virus uses to remain undetected, via its degradation of Pathogen-Associated Molecular Patterns (PAMPs) that would otherwise be recognized …

A Radical Ethnography Of Asian American Mental Health, Sofia Huang

Electronic Theses and Dissertations

Anti-Asian racism and violence in the U.S. both historically and currently impacts the Asian American community, leading to widespread fear and negative mental health consequences for Asian Americans. This has been widely discussed in the media due to the rise in anti-Asian rhetoric and attacks during the COVID-19 pandemic. However, psychological literature does not typically address the effects of the history and politics of Asian America on Asian American mental health. In particular, psychological literature does not engage with radical political perspectives which situate Asian American mental health within the broader context of structural oppression and processes of racialization. This …

Making And Breaking Bonds: Studying Intrinsic Chemistry In The Gas-Phase, Luke Metzler

Electronic Theses and Dissertations

The nature of chemical interactions can be difficult to ascertain in the condensed phase; chemistry can be observed, but with the plethora of variables such as solvation, mixes of counter-ions, and complex equilibria, the true intrinsic chemical properties of a given chemical species may be obfuscated. By conducting research in the gas-phase, many of these variables can be reduced, if not eliminated entirely, which can allow for the careful observation of the true physical phenomena driving chemical reactivity.

The research presented herein focuses on the use of ion-trapping (specifically in a linear ion trap) as a method for unveiling the …

Injectable Formulations For Localized Delivery Of Immunomodulators, Ketki Velankar

Electronic Theses and Dissertations

Dysregulation of immune homeostasis is fundamental to the development of autoimmune disorders. Fibroblastic reticular cells (FRCs) within lymph nodes play crucial roles in forming a microenvironment conducive to immune cell interactions and regulating autoreactive T cells driving autoimmune diseases. Studies have shown the potential of FRCs as a form of personalized cell therapy to correct dysregulated immune responses. Successful translational efforts, however, require the cells to be formulated as injectable suspensions while retaining their native architecture, which is essential for their regulatory functions. The main challenge resides in retaining the native reticular organization of FRCs lost in conventional monolayer cultures …

The Ethical Duty Of Care For Suicide Ideation, Tah Ngijoi Yogo

Electronic Theses and Dissertations

With suicide being a leading global cause of death, there is a need to explore the ethical complexities surrounding suicide and preventive measures. This dissertation questions the morality of suicide by examining the historical and philosophical dimensions across cultures and religions. The text argues for an approach that acknowledges rational suicide while developing the ethics of paternalistic measures for suicide prevention of vulnerable populations.

Considering the ethics of autonomy, the dissertation discusses the practice of nudging in healthcare to highlight the role of paternalism in supporting informed consent. The text proposes an enhanced autonomy model that balances trust, influence, and …

Steroid Sulfatase In Rodent Tissues And A Cell Line: Characterization, Regulation And Inhibition, Barathi Balasubramonian

Electronic Theses and Dissertations

Inactive sulfated steroids are present in plasma at higher concentrations than unsulfated ones. Steroid sulfatase (STS) is the enzyme that removes the sulfate group from sulfated steroid hormones in local tissues, making them active. High levels of STS are linked to breast and prostate cancers, and low levels of STS cause behavioral disorders and X-linked ichthyosis. STS is present in most tissues, yet its physiological role is uncertain. We sought to characterize STS activity in several rodent model systems in order to learn more about its function. Three different sets of experiments were conducted. First, we examined STS in mouse …

Chinese Tallow Long-Term Impact On Stand Dynamics In A Bottomland Hardwood Forest Following Vegetation Management, Nicklaus R. Langlois

Electronic Theses and Dissertations

Chinese tallow (Triadica sebifera (L.) Small) is an invasive tree known to have negative impacts on bottomland hardwood forests. Chinese tallow is proficient in reproducing and surviving in bottomland hardwood forests because it is more flood and shade tolerant than most native trees. The long-term effectiveness of Chinese tallow control is an important topic to research as only a few long-term studies of Chinese tallow control have been conducted. A 10-year re-measurement of 22 paired plots installed in 2012 on the Pineywoods Mitigation Bank near Diboll, Texas following herbicide treatments examined Chinese tallow and native tree stand structure. Each …

Structural Characterization Of The Puma Diapir, Gulf Of Mexico Basin, Rodrigo Velasquez

Electronic Theses and Dissertations

The Gulf of Mexico (GOM) Basin has been a significant target for oil and gas since the 1990s and continues today as a leading exploration target. This super basin is home to an estimated 200 BBOE (Billon barrels of oil equivalent), with current production totaling 60 BBOE for both USA and Mexico. Even though the overall depositional evolution of the GOM has allowed for the key elements for petroleum accumulations to be met, a complex structural framework is present. This framework results from the asymmetric spreading of the North American Plate from the Yucatan block crating the GOM basin we …

The Educational Experiences Of Children And Adolescents With An Ryr1-Related Disease, Jeremy Armann

Electronic Theses and Dissertations

This paper investigates the educational experiences of children and adolescents diagnosed with RYR1-related diseases, a spectrum of congenital myopathies affecting muscle function. Despite these conditions typically sparing cognitive abilities, they profoundly impact physical capabilities, presenting challenges in school environments such as participation in physical activities and social interactions. The study utilizes data collected through the RYR1-Related Disease Educational Impact and Satisfaction Survey (RDEISS) and the Behavioral and Emotional Screening System (BESS) from 49 parents of 51 children aged 3-18 years. Findings reveal prevalent classroom difficulties including handwriting issues and written expression difficulties, with 78% of children receiving special education services, …

The Impact Of Kangaroo Care (Kc) On Parent Stress, Infant Neurobehavioral Regulation, And Parent-Infant Attachment In Very And Extremely Preterm Infants, Justine Vecchiarelli

Electronic Theses and Dissertations

This study examines the high-risk population of infants born atinfants, developmental delays in infants, and parent-infant relationship disruption. Kangaroo care (KC) has shown promise as an intervention to buffer these adverse effects, however, KC engagement in NICUs is obstructed by lack of clear guidelines. Furthermore, limited research exists on KC including infants born atoutcomes, infant behavioral/developmental outcomes, and parent-infant relationship outcomes conjointly. The aim of this study was, therefore, to examine the impact of kangaroo care engagement on precursors of adverse outcomes including parent stress, infant neurobehavioral organization, and parent-infant bonding with the intent of informing clinical practice. Data was …

Effect Of A Systematic Mindfulness Intervention On Student Internalizing, Externalizing, And Adaptive Behaviors, Akshita Nayyar

Electronic Theses and Dissertations

According to Bouchrika (2020), on a scale of 10, American teens rated their stress rate at an average of 5.8, whereas the average stress rate for adults is 3.8. Children with more school problems tend to have more negative and fewer emotions at school and at bedtime (Bai & Repetti, 2017). The most common stressors reported by school-aged children include excessive homework, unrealistic expectations from parents and coaches, peer pressure, standardized testing, and after-school activities (Ryan-Wenger et al., 2005). Schools can address these concerns by providing mental health services within their school settings by collaborating with community agencies within their …

Uptake Of Extracellular Vesicles Into Brain Endothelial Cells And Development Of Motor Neuron-Derived Extracellular Vesicles For Amyotrophic Lateral Sclerosis, Jhanvi Rajan Jhaveri

Electronic Theses and Dissertations

Extracellular vehicles (EVs) are an emerging class of drug carriers and are primarily reported to be internalized into recipient cells via a combination of endocytic routes such as clathrin-mediated, caveolae-mediated and macropinocytosis pathways. In part one of this thesis, (1) we investigated potential effects of homotypic vs. heterotypic interactions by studying the cellular uptake of homologous EVs (EV donor cells and recipient cells of the same type) vs. heterologous EVs (EV donor cells and recipient cells of different types) and (2) determined the route of EV internalization into low pinocytic/hard-to-deliver cell models such as brain endothelial cells ( …

Delivery Of Mitochondria-Containing Engineered Extracellular Vesicles To The Blood-Brain Barrier For Ischemic Stroke Therapy, Kandarp Dave

Electronic Theses and Dissertations

Ischemic stroke is the second leading cause of death worldwide, and the lack of effective treatment options is an unmet clinical need for stroke survivors. Ischemic stroke-induced oxygen-glucose deprivation (OGD) in the affected brain tissue leads to mitochondrial dysfunction and adenosine triphosphate (ATP) depletion in the brain endothelial cells (BECs) lining the blood-brain barrier (BBB). ATP deprivation in the BECs dysregulates actin cytoskeleton dynamics, resulting in BBB breakdown. Restoration of blood flow (i.e., reperfusion) upon the first-line stroke treatment further leads to BBB breakdown and widens the paracellular spaces between BECs. …

Macromolecular Crowding Effects On Enzymes That Display Non-Hyperbolic Kinetics, Kelsey A. Aldrich

Electronic Theses and Dissertations

Macromolecules occupy up to 30% of a cell's volume, yet the in vitro study of biological processes is almost exclusively performed in dilute solutions that fail to approximate the crowded interior of a cell. A growing body of literature has identified that macromolecular crowding has significant effects on a number of biochemical processes, including enzymatic activity. However, changes in kinetic parameters with increased macromolecule concentrations have only been determined for enzymes that follow hyperbolic Michaelis-Menten kinetics, despite the fact that over 25% of enzymes deviate from this model. Here, we perform the first characterization of macromolecular crowding effects on …

The Impact Of “Multiple Looks” When Performing Survival Analysis, Quentin Eloise

Electronic Theses and Dissertations

Survival analysis is a critical statistical method in healthcare to assess patient treatment effects and disease progression. Another critical area of statistical methodology in health care is the practice of adaptive designs. Adaptive designs allow for interim analyses to take place during a study and various decisions and actions can take place more ethically. This is beneficial for studies that take multiple years to complete and allows administrators and healthcare providers to make sound decisions as early as possible. A challenging aspect of adaptive designs is that the number of interim analyses is known in advance which is applicable in …

Rna Secondary Structures: Structure-Function Studies In Viral And Neurodegenerative Diseases, Caylee Cunningham

Electronic Theses and Dissertations

The studies within this dissertation highlight the importance of RNA structure and function, highlighting potential mechanisms in the pathogenesis of the SARS-CoV-2 virus and hallmarks of ALS. Specifically, the s2m, a 41-nt structure found within the hyper variable region of the 3’ UTR of the SARS-CoV-2 virus, was investigated as this element has no definitive role in viruses but its high conservation in several viral families warrants investigation. We used various biophysical techniques to determine if a palindromic sequence in the terminal loop could participate in dimerization activities, which may have implications in recombination and the longevity of the virus. …

Furthering Ascidian Taxonomy Using Molecular Biology, Nicholas Gulnick

Electronic Theses and Dissertations

Ascidians are our closest invertebrate relatives and comprise nearly 3,000 species separated into three orders: Aplousobranchia (most speciose), Stolidobranchia, and Phlebobranchia (least speciose). Ascidians can be classified as either solitary or colonial organisms. Species delimitation using morphological characters alone has had varied results. Well known, widely distributed, morphological species have turned out to be catch all species comprised of several cryptic species. Molecular markers can help mitigate some of the issues presented by strictly using morphological observations, including resolving the status of cryptic species, and accessing the expert knowledge required to identify a species. By incorporating molecular markers and pairing …

Variability In Nutrient Concentrations Across Regions Of The Winyah Bay River Plume, Anne Mackenzie Gossman

Electronic Theses and Dissertations

River plumes are one of the few large-scale features responsible for transporting particulates and terrigenous materials to coastal oceans. Macro-nutrients, such as dissolved inorganic nitrogen (DIN) and phosphorus (PO43-), are basic requirements for marine food webs. Due to the plumes’ ability to transport large quantities of these constituents, these systems contribute heavily to coastal productivity. This study focuses on the dynamics of the Winyah Bay river plume located in Georgetown, South Carolina. River plumes, including the Winyah Bay plume, are dynamic systems whose structures are comprised of a multitude of regions and features including the source, lift off, near-field, mid-field, …

College Students' Perceptions Of The Student-Teacher Relationship, Lauren E. Biggs

Electronic Theses and Dissertations

Humans, as social creatures, pursue relationships with others to fulfill various needs. As such, when we enter school, we naturally seek to establish relationships with teachers, who act as significant individuals in our lives. In general, research has shown that students with close, warm relationships with their teachers tend to experience more favorable outcomes related to academic achievement, behavior, and social-emotional factors. Students with more negative relationships with their teachers conversely tend to experience poorer outcomes. Though decades of research have consistently shown this correlation, few studies have examined the relationships between college students and their professors. The current study …

Avian Communities Monitored Along A Successional Chronosequence In Bottomland Hardwood Forests In The Lower Mississippi Alluvial Valley, Eamonn Alasdair Thurmond

Electronic Theses and Dissertations

Bottomland hardwood forests in the Lower Mississippi Alluvial Valley have been extensively reduced due to agriculture conversion and altered hydrology. Remnant forests have undergone further degradation from sources such as a lack of forest management, invasive species, and reduced regeneration of oaks (Quercus spp.). Recent restoration efforts of bottomland hardwood forests have focused on implementing wildlife forestry practices that prioritize restoring wildlife habitat while retaining profitable timber harvests. In this study I surveyed bird communities in bottomland hardwood forests in the Lower Mississippi Alluvial Valley along a successional chronosequence. Birds were sampled using automated recording units over 10 days …

Manipulation Of Cellular Proliferating Cell Nuclear Antigen (Pcna) During Herpes Simplex Virus Type 1 Infection, Jessica Packard, Jill A. Dembowski

Electronic Theses and Dissertations

Herpes simplex virus type 1 (HSV-1) is a double stranded DNA virus that replicates within the nucleus of host cells. Using accelerated native isolation of proteins on nascent DNA (aniPOND), cellular proteins were found to associate with HSV-1 replication forks. One host factor of interest is proliferating cell nuclear antigen (PCNA), the DNA sliding clamp that tethers DNA polymerases and DNA damage response proteins to replicating cellular DNA. Although HSV-1 encodes its own DNA polymerase processivity factor (UL42), PCNA plays a key role in HSV-1 infection. We used commercially available PCNA inhibitors and short hairpin RNAs (shRNA) to investigate how …

Structure-Function Relationships In Neurodegenerative And Infectious Diseases: Biophysical Characterization Of Rna Secondary Structures By Computational Techniques, Kendy Guarinoni

Electronic Theses and Dissertations

The research described in this dissertation focuses on the investigation of the structure and dynamics of regions of RNA implicated in two diseases: COVID-19 and amyotrophic lateral sclerosis/frontotemporal dementia (ALS/FTD). Both studies sought to create experimentally corroborated models that were used to characterize the structure and dynamics of the C9orf72 repeat expansion and the s2m region in coronaviruses, respectively and provided the foundation for future work. In our work involving the s2m region of SARS-CoV-2, the virus responsible for COVID-19, we determined that the homology modeling approach commonly used to derive atomistic structures when no coordinates are available yields a …

Environmental Factors (Rurality, Distribution Of Prescribers, And Altitude) Of People Who Died By Suicide In Montana, 2013- 2022, Anne N. Brown

Electronic Theses and Dissertations

Introduction: Suicide is increasingly prevalent in rural areas across the United States, especially in the western Rocky Mountain states. The connection between suicide and rural residency is not well understood, nor is the relationship between the distribution of prescribers, altitude, and suicide. Purpose: This retrospective quantitative study aimed to describe the characteristics of individuals who died by suicide in Montana and investigate the associations among rurality, distribution of prescribers, altitude, and suicide rate. Methods: Montana suicide mortality data for 2013-22 was used to describe individual characteristics. A second dataset was created for each Zip Code Tabulation Area (ZCTA) level in …

Emotionality Stigma Scale: Measurement Development, Reliability, And Validity., Hayley D. Seely

Electronic Theses and Dissertations

Emotions are biological responses to stimuli that allow individuals to derive meaning, appraise experiences, and prepare to respond. However, individuals perceive emotions differently based on emotion socialization which not only dictates the way emotions are viewed and managed but also has been directly linked with mental health outcomes. Furthermore, research shows emotion socialization is informed by demographic variables such as gender such that the expectations of emotionality differ; where women are taught to express emotions, men are taught to conceal. Given the societal rules regarding emotionality, it is possible that emotionality stigma – the stigma around the experience and expression …