Digital Commons Network^™

Protein Aggregation Through Acoustic Cavitation, John A. Giarratano

Electronic Theses and Dissertations

Therapeutic proteins represent an essential piece of a health management plan for diseases such as diabetes, cancer, hemophilia, Crohn's Disease, and myocardial infarction. These proteins, however, must be maintained in their correct, biologically active conformation throughout processing, transportation, and delivery. This requirement poses serious engineering challenges because of a protein's susceptibility to thermodynamic instabilities resulting from the weak bonds driving the tertiary structure of the molecule. A particularly problematic type of protein degradation is aggregation. Administration of aggregated proteins, a particularly problematic degradation form, can have dire consequences, including blocking a patient's responsiveness to therapy, inducing immunogenicity, and even anaphylactic …

Posthumanist Rhetorical Agency, Matt Dunn

Electronic Theses and Dissertations

The postmodern criticism of humanist agency initiated by Dilip Gaonkar nearly twenty years ago set in motion a discipline wide discussion concerning the conceptualization rhetorical agency. Rhetorical agency is difficult but vital to conceptualize because the term bears directly on the discipline's theorizing about the speaker or rhetor, the effect of the speaker or rhetor's rhetoric on an audience, and the extent to which the speaker or rhetor's agency is constrained by ideology and discourse. What emerged from this discussion about agency did distance the discipline from the humanist conceptualization of rhetorical agency that persisted at the time Gaonkar published …

Connexin-32 And Connexin-43 Immunoreactivity In Rodent Taste Buds, Amanda E. Bond

Electronic Theses and Dissertations

Studies indicate that ATP is one of the primary neurotransmitters in taste transduction. ATP release occurs from taste cells via specific hemichannels such as pannexin/connexin hemichannels (Huang et al., 2007; Romanov et al., 2007). We hypothesize that Type II (receptor) and possibly Type III (presynaptic) cells release ATP at sites containing pannexin/connexin hemichannels. In this study, we examine the presence of connexin–32–LIR (Like Immunoreactivity) and connexin–43–LIR in rodent taste buds through immunocytochemical analysis and DAB (Di–amino–benzidine) immunoelectron microscopy. We observed that connexin–32–LIR co–localizes with P2X2–LIR in nerve fibers and in a small subset of NCAM–LIR cells. Connexin–32–LIR does not co–localize …

Fostering And Foreclosing Student Learning Potential: Portraits Of Performativity, Emotion, And Relationality In The Classroom, Jeanne Marie Jacobs

Electronic Theses and Dissertations

This dissertation endeavors to build a much-needed bridge between the fields of communication and education. Using critical pedagogy and critical communication pedagogy as theoretical frameworks, this project advances an understanding of classroom communication as constitutive of power relations, and teachers and students as agents who can work together to foster learning potential and social justice. I look to interdisciplinary scholarship on affect to craft a nuanced conceptual framework of the connection between communication and emotion, and how they create learning opportunities for some students and construct barriers to learning for others. Through ethnographic fieldwork at an urban magnet school, I …

Design And Synthesis Of Novel Fluorescent Nucleoside Analogues, Nada Abdulnasir Elsharif

Electronic Theses and Dissertations

This project aimed to obtain a set of fluorescent nucleoside analogues that closely resemble cytosine with extending the aromatic surface (extended conjugation) for enhance the photophysical properties. In addition to synthesize new fluorescent nucleoside analogues and evaluate their photophysical properties, this project aimed to understand the effects of donor substituent on the tC photophysical properties.

The main goal of these studies is to develop highly fluorescent nucleotide analogues. We first were looking to synthesize tC^C fluorescent nucleotide with the pyrimido[4,5-b]quinolone. In this part, 2-chloro-3- formylquinoline was easily prepared, yet the cyclization of 2-chloro-3- formylquinoline with urea or guanidine HCl …

An Emerging Hispanic Serving Institution Looks Toward The Future: A Case Study On A Collaborative, Community-Based Systemic Change Effort At An Urban Land-Grant College, Eric Dunker

Electronic Theses and Dissertations

Many higher education organizations face the issue of structural isolation (Ruben, 2004) based on a lack of integration and functional coordination between faculty, staff, and students. Community-based research (CBR) offers higher education a more collaborative, wider reaching avenue of organizing a variety of stakeholders around social change. The CBR model is an atypical change model due to its interdisciplinary, participatory, and collaborative structure that values multiple sources of knowledge and focuses on social justice action. As opposed to serving as the 'experts' who were performing research on a community of people, academics and community members who utilize CBR focus on …

Carbon Nanotube Biosensors For Detection Of Biomarkers In Breast Cancer Cells., Benjamin C. King

Electronic Theses and Dissertations

Detection and profiling of circulating tumor cells (CTCs) is useful for cancer screening and for managing treatment of carcinoma patients. Label-free technologies aim to accomplish detection rapidly with small, simple micro and nano devices. Carbon nanotubes are favorable molecular sensors due to their unique properties. They have been widely investigated for immunosensing of cancer biomarkers as free proteins, but very little has been done to detect biomarkers in intact cells and much remains to be understood regarding the mechanism of their sensing. We have developed a simple carbon nanotube biosensor for epithelial cell adhesion molecule (EpCAM) for sensing EpCAM positive …

Weight Concerns, Body Image, And Smoking Cessation In Pregnant Women In Rural Appalachia, Jennifer Aileen Correll

Electronic Theses and Dissertations

Smoking is the leading preventable cause of death, and smoking during pregnancy can lead to significant health complications for both the mother and developing child. In the Appalachian region of East Tennessee, pregnancy smoking rates are as high as 40% in some counties which is almost 4 times the national average. Weight and body image concerns have previously been identified as variables contributing to maintenance of smoking behavior. The current study was designed to examine the relationship between prepregnancy weight and body image concerns and smoking status at third trimester. Participants included 172 pregnant women who smoke. Women were recruited …

Healthcare Technology: A Strategic Approach To Medical Device Management, Chad A. Kinley

Electronic Theses and Dissertations

The constant evolution of medical technology has increased the demand for managing medical devices to ensure safety and effectiveness. In this paper I will investigate how biomedical engineering has addressed the issue of equipment management and identifies strategies to successfully maintain an inventory of medical devices. Through research, on-the-job experience, and in-depth discussions with various biomedical engineering managers, I have been able to document possible equipment strategies and best practices for managing medical devices. There is really no "one size fits all" to medical equipment management due to the various clinical environments, but there are many aspects that remain necessary …

Reflex Of Avoidance In Spatial Restrictions For Signatures And Handwritten Entries, Linde Christine Rush Burkey

Electronic Theses and Dissertations

Regarding the myriad disputed documents encountered within the science of forensic document examination, questioned handwriting is the most prevalent. This includes the simulation or alteration of and or additions to handwriting and signatures. The current study examined the changes that may occur in writing when given a limited amount of space. Several participants completed a survey wherein writing samples were taken under varying space allowances. These space restrictions were made under differing conditions such as boxed signatures, additions to prewritten material, and alterations to letters and numbers. The results of the study found characteristics of reflex of avoidance in the …

Relationships Between Reading Ability In Third Grade And Phonological Awareness In Kindergarten, Melissa Lynn Pannell

Electronic Theses and Dissertations

The purpose of this study was to identify relationships that exist between reading ability in 3rd grade and phonological awareness in kindergarten. A second purpose was to identify specific prereading skills that best predict later reading success. This study used a quantitative research design to answer the research questions posed. The population for this research was 244 fourth grade students enrolled in 3 primary schools in a school system in Southwest Virginia. The data used for this research study were obtained from each student's score on the kindergarten Phonological Awareness and Literacy Screening and the 3rd grade Virginia Standards of …

A Consensus Model For Electroencephalogram Data Via The S-Transform, Andrew Coady Young

Electronic Theses and Dissertations

A consensus model combines statistical methods with signal processing to create a better picture of the family of related signals. In this thesis, we will consider 32 signals produced by a single electroencephalogram (EEG) recording session. The consensus model will be produced by using the S-Transform of the individual signals and then normalized to unit energy. A bootstrapping process is used to produce a consensus spectrum. This leads to the consensus model via the inverse S-Transform of the consensus spectrum. The method will be applied to both a control and experimental EEG to show how the results can be used …

The Effects Of Nicotine Administration On Behavior And Markers Of Brain Plasticity In A Rodent Model Of Psychosis, Marla K. Perna

Electronic Theses and Dissertations

Schizophrenia affects about 1% of the population. A hallmark of the disorder is increased dopamine D2 receptor sensitivity in the brain. Studies have shown that schizophrenics smoke cigarettes at approximately 4 times the rate of the general population. It has been suggested that nicotine use is a form of self-medication for symptoms in schizophrenia. Smoking behaviors typically begin in adolescence. We assessed effects of nicotine on behavior and brain plasticity in an adolescent rodent model of schizophrenia with the goal of identifying targets for smoking cessation. Methods: Rats were neonatally treated with quinpirole (a D2/D3 agonist) or saline and sensitized …

Effects Of Burn Injury On Biological Ethanol And Ethyl Glucuronide Concentrations, Trista Haupt Wright

Electronic Theses and Dissertations

Alcohol is the most abused drug in the United States and most frequently performed assay in forensic laboratories. Alcohol is routinely present in biological specimens from fatal residential fires and forensic toxicologists must interpret if these individuals are impaired by determination of their blood alcohol concentrations on post-incineration blood collected at autopsy. There is no known data available to confirm or refute blood alcohol concentrations and impairment in fire-related deaths. Ethyl glucuronide (EtG), a non-volatile minor ethanol metabolite, may provide a better biomarker for ethanol consumption prior to burn injury. The literature does not address the possibility that ethanol or …

Studies On The Preparation, Immobilization, And Luminescence Properties Of Zinc Oxide (Zno)Quantum Dots, Yasemin Hakat

Electronic Theses and Dissertations

Quantum dots are a part of very important our technological future because of their intriguing and useful properties. These properties are different in character from those of the corresponding bulk material. Quantum dots are inorganic artificial semiconductor nanocrystal whose electrons influence their physical and chemical properties. Zinc oxide quantum dots were synthesized through an ethanol based precipitation via colloidal synthesis method at various pH values. Various emission colors were obtained because the excited quantum dots of various sizes emitted specific wavelengths of light. The emission spectra indicated that the pH dependent quantum dots were successfully synthesized. Zinc oxide quantum dots …

Exploring The Unique Characteristics Of Cancer In Adolescents And Young Adults In Tennessee, Megan Quinn

Electronic Theses and Dissertations

Adolescents and Young Adults (AYAs) ages 15-39 years with cancer have received little attention in the medical and health fields, resulting in a lack of progress for this age group. Little is known about the unique biologic, epidemiologic, and psychosocial issues that play an integral role in the AYA cancer journey. The purposes of this study were to use the Tennessee Cancer Registry for all new cancer cases from 2004-2008 to determine 1) the main types of cancer that affect AYAs in TN, 2) the predictors of late-stage diagnosis of melanoma, and 3) the factors that predict a total thyroidectomy …

‘Talking’ During Early Romantic Courtship: An Empirical Examination Of Potential Sex Differences In Self-Reported Beliefs And Behaviors, Eric James Anderson

Electronic Theses and Dissertations

This thesis tests various hypotheses from a variety of research traditions that predict the likelihood for potential sex differences in "talking": a newly-emergent phase of romantic courtship. Data for this study was derived from a purposive sample of 566 students enrolled during the Fall 2011 semester generated using a self-administered survey available on the East Tennessee State University SONA system. Statistical analyses using chi-square, analysis of variance (ANOVA), and binary logistic regression reveal statistically significant differences for males and females on beliefs about sexual exclusivity and sexual activity during the "talking" phase. Significant behavioral differences exist in whether "talking" is …

Student Success In Face-To-Face And Online Sections Of Biology Courses At A Community College In East Tennessee, Deanna Essington Garman

Electronic Theses and Dissertations

The purpose of this quantitative study was to determine if there were significant differences in student success in face-to-face and online biology courses as categorized by gender, major, and age; and as measured by lecture grades, lab grades, and final course grades. The data used for analyses included data from 170 face-to-face sections and 127 online sections from a biology course during the fall and spring semesters beginning fall 2008 through spring 2011.

Researchers have reported mixed findings in previous studies juxtaposing online and face-to-face course delivery formats, from no significant differences to differences in grades, learning styles, and satisfaction …

The Invisible Enemy: The Effects Of Polio On The American War Effort During World War Ii, 1941-1945, Jacob Owen Bryant

Electronic Theses and Dissertations

This thesis looks at the social, political, and military effects of epidemic polio on America's war effort during World War II. The primary sources consulted include newspapers, military medical reports, photographs, memoirs, speeches, and archival collections. It looks at the effects of polio on the home front, more specifically how epidemics and the rising rates of polio were a detriment to the civilian war effort. It also focuses on the American military's preparation for and response to polio outbreaks among troops both at home and abroad. Finally, it discusses the experiences of the servicemen who contracted polio during the war. …

An Investigation Of Bacterial Ribonucleases As An Antibiotic Target, Ashley Denise Frazier

Electronic Theses and Dissertations

Antibiotics have been commonly used in medical practice for over 40 years. However, the misuse and overuse of current antibiotics is thought to be the primary cause for the increase in antibiotic resistance.

Many current antibiotics target the bacterial ribosome. Antibiotics such as aminoglycosides and macrolides specifically target the 30S or 50S subunits to inhibit bacterial growth. During the assembly of the bacterial ribosome, ribosomal RNA of the 30S and 50S ribosomal subunits is processed by bacterial ribonucleases (RNases). RNases are also involved in the degradation and turnover of this RNA during times of stress, such as the presence of …

End Use Packaging: A Survey Of Customer Perception Of Recycling And Environmental Impact Of Packaging Materials, Whitney Chelsea Lamb

Electronic Theses and Dissertations

Studies have shown that traditional food packaging for the fast food industry leach toxic chemicals into the food products. Some of these toxins are known endocrine disruptors, which results in reproductive issues as well as hormone imbalances. Alternatives to traditional food packaging have been addressed with special attention to bioactive, biopolymers, and biodegradable packaging in addition to active and intelligent packaging. The hypothesis states that customers will have high demand for more environmentally sustainable fast food packaging, while the alternative hypothesis states that demand will not be high enough to financially support the costly introduction of more environmental packaging products. …

Bayesian Nonparametric Clusterings In Relational And High-Dimensional Settings With Applications In Bioinformatics., Dazhuo Li

Electronic Theses and Dissertations

Recent advances in high throughput methodologies offer researchers the ability to understand complex systems via high dimensional and multi-relational data. One example is the realm of molecular biology where disparate data (such as gene sequence, gene expression, and interaction information) are available for various snapshots of biological systems. This type of high dimensional and multirelational data allows for unprecedented detailed analysis, but also presents challenges in accounting for all the variability. High dimensional data often has a multitude of underlying relationships, each represented by a separate clustering structure, where the number of structures is typically unknown a priori. To address …

Alternative Performances Of Race And Gender In Hip-Hop Music : Nerdcore Counterculture., Jessica Elizabeth Ronald

Electronic Theses and Dissertations

In his documentary entitled, Nerdcore For Life, director Dan Lamoureux described Nerdcore as a "powerful social collision between hip hop and geek culture". Born on the Internet, Nerdcore Hip-Hop is rap music made by geeks, for geeks and covers such traditionally "nerdy" topics such as comic books, video games, science fiction, anime, technology, etc. Though it has existed online for almost a decade, only recently has Nerdcore gone from being an Internet fad to an underground cultural phenomenon. This paper investigated how hegemonic constructions of race and gender within both the dominant public sphere and hip-hop culture are subverted and …

The Regulation Of Rna Polymerase I-Mediated Transcription In Forebrain Neurons., Scott Carl Smith

Electronic Theses and Dissertations

Ribosomal biogenesis failure may contribute to neurodegenerative diseases, while its excessive activation has been shown to drive tumor growth. As ribosomal production is initiated and regulated by the Pol1-mediated transcription of rRNA genes in the nucleolus, the latter process had the attention of most researchers interested in the dysregulation of ribosomal biogenesis in disease. When this work began, regulation of Pol1-mediated transcription in neurons had been poorly characterized. The goal of this research has been to better define factors and signaling pathways that regulate neuronal Pol1 activity. The first hypothesis tested was that DNA damage induced by a DNA topoisomerase …

Expression Of Adipocyte/Macrophage Fatty Acid Binding Protein Promotes Tumor Growth And Metastasis., Ashley Simone Triplett

Electronic Theses and Dissertations

It has been estimated that 30% of all cancer deaths in the U.S. are associated with obesity. It is well-established that obesity promotes low-grade chronic inflammation, however the mechanisms by which obesity-induced chronic inflammation may promote cancer development and progression are not well-defined. Fatty acid binding proteins (FABPs), which are intracellular lipid chaperones, regulate both metabolic and inflammatory pathways. Of the nine FABP family members, adipocyte/macrophage-FABP (A-FABP) has been found to be highly expressed in macrophages in both mice and humans and its expression is increased in response to a high-fat diet. In the present study we examined the influence …

Of Mice And Sigma : Conferred Antibiotic Resistance In The Salmonella Enterica Serovar Typhimurium Murine Model., Rena Margaret Wallen 1981-

Electronic Theses and Dissertations

Salmonella enterica serovar Typhimurium is an enteric pathogen capable of infecting a wide range of hosts. The manner in which this pathogen is able to interact with its host is difficult to define, as is the case with most microbes. Through the use of alternate sigma factors and other regulatory processes, S. Typhimurium is able to invade host cells to establish systemic infections, and survive the assaults of the host immune system. While most strains of S. Typhimurium are typically ampicillin sensitive, within the host, survival inside host cells may provide an escape from many antibiotics. Previous research demonstrated that …

Structure-Activity Relationship Model For Estrogen Receptor Ligands., Huihui Wu 1979-

Electronic Theses and Dissertations

Xenoestrogens are spread throughout the environment affecting our daily lives and may produce potential toxic effects on human health. The purpose of this study was to develop a mechanistically reliable model capable of identifying xenoestrogens. Our hypothesis was that there are identifiable structural characteristics among a diverse set of estrogen receptor ligands that differentiate estrogenic and nonestrogenic compounds. The model's learning set was developed by collecting compounds from the National Center for Toxicological Research Estrogen Receptor Binding database (NCTRER) . The categorical-SAR (cat-SAR) expert system was used to build the models and perform leave-none-out, leave-one-out, leave-many-out and external validations for …

The Impact Of Adsorbed Cellulase Inactivation On Enzymatic Hydrolysis Kinetics., Zhuoliang Ye

Electronic Theses and Dissertations

Several technical and economic obstacles currently hamper the industrial development of ethanol from biomass. One of the key bottlenecks is the slow kinetics of the enzymatic hydrolysis of cellulose, and the subsequent rate reduction as the reaction proceeds. As a result, this research focused on understanding underlying causes for the slow kinetics, rate reduction, and low yield during cellulose hydrolysis. Mechanisms traditionally thought to cause these results were investigated, such as change of substrate properties and deactivation of enzyme due to environmental mechanisms, but neither was found to contribute significantly to the slow kinetics and low yield. Inactivation due to …

Click Scaffolds For The Inhibition Of Porphyromonas Gingivalis And Streptococcus Gordonii Biofilm Formation., Catherine Marie Loner

Electronic Theses and Dissertations

Periodontitis and its systemic sequelae remain a major public health problem and developing a cost-efficient therapy will benefit healthcare worldwide. Adherence of Porphyromonas gingivalis to Streptococcus gordonii facilitates colonization of the oral cavity by P. gingivalis and contributes to development of periodontal disease. It has been demonstrated that synthetic peptides (BAR-peptides) inhibit this interaction and the formation of P. gingivalis biofilms. However, peptides are susceptible to proteolytic degradation thereby precluding their use as therapeutic agents. Consequently, rationallydesigned small-molecule peptidomimetics of BAR will potently inhibit P. gingivalis adherence to S. gordonii and represent viable therapeutic compounds. In terms of inhibitor design, …

A Spatial Age-Structured Model Of Perennial Plants With A Seed Bank., Kimberly Ilene Meyer

Electronic Theses and Dissertations

We formulate an integro-difference model to predict the growth and spatial spread of a perennial plant population with an age-structured seed bank. We allow the seeds in the bank to be of any age, producing an infinite system of equations. The production of new seed can be density-dependent and so the function describing this growth is allowed to be non-monotone. The functions describing the seed bank are linear. We develop properties about the non-spatial model, including the existence of a positive steady-state and conditions under which solutions converge to this steady-state. We also show that when the origin is unstable, …