Digital Commons Network^™

Genetically Modified Food In The Us And The Uk: Proponents, Dissenters And Media Coverage, Tess Ann Doezema

Electronic Theses and Dissertations

This research is a qualitative study examining the communication surrounding the issue of genetically modified food in the UK and the UK from October, 2011 through September, 2012. Material from biotechnology industry organizations, industry-funded non-profits, groups campaigning against the continued use of the technology, and mainstream media coverage of the issue in both countries during this time was examined using thematic analysis. The issue is analyzed through the lenses of Herman and Chomsky's propaganda model, agenda building and framing theory. The research finds support for agenda building as well as a modernized understanding of the propaganda model, which the researcher …

Electricity Markets, Smart Grids And Smart Buildings, Jonathan Falcey

Electronic Theses and Dissertations

A smart grid is an electricity network that accommodates two-way power flows, and utilizes two-way communications and increased measurement, in order to provide more information to customers and aid in the development of a more efficient electricity market. The current electrical network is outdated and has many shortcomings relating to power flows, inefficient electricity markets, generation/supply balance, a lack of information for the consumer and insufficient consumer interaction with electricity markets. Many of these challenges can be addressed with a smart grid, but there remain significant barriers to the implementation of a smart grid.

This paper proposes a novel method …

Novel Functions For Neuronal Rna Processing Bodies In The Control Of Axon Terminal Growth In Drosophila Melanogaster, Sarala Joshi Pradhan

Electronic Theses and Dissertations

In this thesis we first characterized neuronal functions for HPat/Pat1, a core component of RNA processing bodies or "P bodies". We show that hpat mutants exhibit a strong synaptic hyperplasia at the developing and acutely stimulated Drosophila larval neuromuscular junctions (NMJs). The synaptic defects observed in hpat mutants are associated with rearrangement of the axonal microtubule cytoskeleton suggesting that HPat negatively regulates presynaptic microtubule-based growth during NMJ development. Interestingly, we also found that both pre-and postsynaptic HPat expression controlled rapid axon terminal growth in response to acute spaced synaptic stimulation. We also demonstrate that HPat interacts genetically with the catalytic …

Adolescents Who Stutter: Perception Of Effective Therapy Techniques, Megan Weigel

Electronic Theses and Dissertations

Stuttering is considered a low-incidence communication disorder, but for those who stutter, the symptoms can handicap many aspects of life. Stuttering typically presents early in childhood and data indicates the probability for natural recovery ends near the emergence of adolescence. When stuttering continues into adulthood, it is considered chronic. Currently, there is limited research on which therapy techniques have proven to be most successful with adolescents, a unique population at a pivotal age in therapy. The original intent of this study was to investigate factors that contribute to successful therapy for adolescents who stutter. Due to recruitment limitations, the data …

Synthesis And Molecular Modeling Studies Of Bicyclic Inhibitors Of Dihydrofolate Reductase, Receptor Tyrosine Kinases And Tubulin, Sudhir Raghavan

Electronic Theses and Dissertations

The results from this work are reported into two sections listed below:

Synthesis:

Following structural classes of compounds have been designed, synthesized and studied as inhibitors of pjDHFR, RTKs and tubulin:

1. 2,4-Diamino-6-(substituted-arylmethyl)pyrido[2,3-d]pyrimidines

2. 4-((3-Bromophenyl)linked)-6-(substituted-benzyl)-7H-pyrrolo[2,3-d]pyrimidin-2-amines

3. 6-Methyl-5-((substitutedphenyl)thio)-7H-pyrrolo[2,3-d]pyrimidin-2-amines

A total of 35 new compounds (excluding intermediates) were synthesized, characterized and submitted for biological evaluation. Results from these studies will be presented in due course. Bulk synthesis of the potent lead compound 170 was carried out to facilitate in vivo evaluation.

Docking Studies

Docking studies were performed using LeadIT, MOE, Sybyl or Flexx for target compounds listed above and for other compounds …

Theological Foundations For An Ethics Of Cosmocentric Transfiguration: Navigating The Eco-Theological Poles Of Conservation, Transfiguration, Anthropocentrism, And Cosmocentrism With Regard To The Relationship Between Humans And Individual Nonhuman Animals, Ryan Patrick Mclaughlin

Electronic Theses and Dissertations

In the past forty years, there has been an unprecedented explosion of theological writings regarding the place of the nonhuman creation in ethics. The purpose of this dissertation is to propose a taxonomy of four paradigms of eco-theological thought that will categorize these writings and facilitate the identification, situation, and constructive development of the paradigm of cosmocentric transfiguration. This taxonomy takes shape within the tensions of three theological foundations: cosmology, anthropology, and eschatology. These tensions establish two categorical distinctions between, on the one hand, conservation and transfiguration, and, on the other, anthropocentrism and cosmocentrism. The variations within these poles yield …

Gourmet Food Trucks: An Ethnographic Examination Of Orlando's Food Truck Scene, Zachary Hawk

Electronic Theses and Dissertations

Gourmet food trucks have emerged as increasingly popular dining alternatives for consumers in today’s urban landscape. Existing literature, as well as my own ethnographic research within Orlando, Florida’s mobile food vending scene, reveals that food truck owner/operators utilize various strategies to establish a viable niche for themselves in this diversified and burgeoning market. Among other things, these strategies include online social networking, creating and maintaining a recognizable brand identity, collaborating with local retailers and bar owners, and incorporating organic and locally produced ingredients in their dishes whenever possible. As in other parts of the country, there appears to be a …

A Role For Dynamin-Dependent Endocytosis During Drosophila Gastrulation, Marissa Kay Kuhl

Electronic Theses and Dissertations

Gastrulation, a process conserved among many higher organisms, is the directed migration of cells into layers that will establish various tissues targeted to become anatomical structures. This process is accomplished through another conserved morphogenetic event, known as cell intercalation. Early in development, this movement of cells within an organized tissue leads to unique cellular arrangements where neighboring cells contract their shared interfaces in order to meet at a shared vertex. In this thesis, I present work that demonstrates a requirement for Dynamin-dependent endocytosis during these contraction events. Using quantitative analysis, I have identified varied cell behaviors during experiments which knockdown …

Longitudinal Effects Of Improving Inter-Parental Relationships In Low-Income Couples: Child Outcomes, Erica Grace Moran Etter

Electronic Theses and Dissertations

Poverty and economic hardship create tremendous stress for families, and subsequently are risk factors for child psychopathology. The Fatherhood, Relationship, and Marriage Education (FRAME) program is a 14-hour psychoeducation intervention developed specifically to strengthen the ability of low-income mothers and fathers to reduce conflict, cope with stress, and co-parent effectively, hopefully helping to create more stable and secure environments for children. The FRAME study is a randomized controlled trial evaluating the efficacy of a couples-based intervention in a sample of 301 ethnically diverse low-income cohabiting mothers and fathers who are parenting at least one child together. Couples were randomly assigned …

A Mixed Methods Examination Of Structural Bigenderism And The Consequences For Transgender And Gender Variant People, Kristie Lynn Seelman

Electronic Theses and Dissertations

For years, transgender activists and their allies have spoken out about the oppression that transgender and gender non-conforming people experience in relation to societal systems and institutions, due to policies and practices that do not acknowledge non-binary experiences of gender, that do not recognize that one's gender may change over time or may not match cultural expectations for gender expression, and that punish and discriminate against trans people (Gilbert, 2009; Lombardi & Davis, 2006; Markman, 2011; Spade, 2006; WWRC, 2010). Scholars have called for human services professionals and researchers to critique the failures of institutions in society (and the people …

Analysis Of The Scale Of Parenting And Life Functioning Using The Rasch Measurement Model, Laricia Longworth-Reed

Electronic Theses and Dissertations

The purpose of the current study was to examine dimensionality, reliability, invariance, targeting, scale use and functioning of items across subgroups for a sample of 278 on the Scale of Parenting and Life Functioning (SPLF). Analysis was conducted using a Rasch partial credit model. Overall, the SPLF showed good overall fit to the model. Unidimensionality of the scale was supported in the analysis, with over 60% of the raw variance explained by the measure. Item fit also provided evidence that the items fit the model. Scale categories displayed clear monotonicity. No differential item functioning was found for mother's age, race/ethnicity, …

Biophysical Approaches To Human Genetic Disease: Adsl Deficiency As A Model, Stephen P. Ray

Electronic Theses and Dissertations

Biophysical tools are becoming more useful in approaching human genetic disease. The pathogenic mechanisms behind many disorders have been attributed to protein loss of function or gain in toxic function. For example, changes in the protein Adenylosuccinate Lyase (ADSL) lead to ADSL deficiency, a disorder that causes symptoms ranging from epilepsy to expression of autistic features. Biophysics offers different tools to study intrinsic properties of proteins. We have applied such tools to study the enzyme kinetics and protein stability of ADSL and two mutations (R426H and R303C) to formulate better hypotheses regarding the pathogenic mechanism. Enzyme kinetic findings indicate a) …

Learning Hierarchical Representations For Video Analysis Using Deep Learning, Yang Yang

Electronic Theses and Dissertations

With the exponential growth of the digital data, video content analysis (e.g., action, event recognition) has been drawing increasing attention from computer vision researchers. Effective modeling of the objects, scenes, and motions is critical for visual understanding. Recently there has been a growing interest in the bio-inspired deep learning models, which has shown impressive results in speech and object recognition. The deep learning models are formed by the composition of multiple non-linear transformations of the data, with the goal of yielding more abstract and ultimately more useful representations. The advantages of the deep models are three fold: 1) They learn …

Provider Perceptions Of Ableism And Social Support Networks In The Healthcare Setting, Darcey Mccampbell

Electronic Theses and Dissertations

The objective of this study is to investigate ableism and social support networks in the healthcare setting. Social support networks play an important role in combating emotional distress in healthcare. They provide disabled patients a method for defending against the negative effects of ableism among other stressors. By definition, ableism refers to perceptions that disability is abnormal and undesirable. Ten healthcare providers in central Florida (i.e., in nursing homes, rehabilitation centers, and primary care offices) were interviewed, using in-depth face-to-face qualitative interviewing. Participants were asked questions relating to the effects of ableist language on patients with disabilities as well as …

Husserl And Foucault On The Subject: The Companions, Harry Nethery Iv

Electronic Theses and Dissertations

In this text, I argue for the revision of Husserlian phenomenology through a dialogue with the work of Michel Foucault. Specifically, I argue that Foucault's critical project, in which we isolate the contingent limits of thought so as to pass beyond them, and thus think new ways of being, can be filled out by the work of Edmund Husserl and differentiated into two lines of inquiry: a critical ontology and a critical phenomenology. This is accomplished by bringing these two philosophers, commonly held to be diametrically opposed, into dialogue such that together they say something that neither could say on …

Differential Expressions Of Nodal-Signal Transducers And Global Transcriptional Repression Commit Vegetal Cells In Eleutherodactylus Coqui To Form Nutritional Endoderm, Suman Chatterjee

Electronic Theses and Dissertations

The vegetal core cells of a Xenopus laevis embryo commit to mesendoderm via the Nodal-signaling pathway. In Eleutherodactylus coqui, a direct developing frog, mesendoderm is specified at the marginal zone of the early gastrulae and vegetal core cells transform into nutritional endoderm. It is a novel tissue consisting of transient, yolk rich cells that provide nutrition, but do not differentiate into adult tissues. We hypothesized that a disruption of Nodal-signaling is responsible for committing vegetal core cells to nutritional endoderm. I report a dual regulation involved in the generation of nutritional endoderm. First, differential expression of Nodal-signaling components like Smad2 …

Studies Of Mercury In Water, Sediment, And Fish In Mississippi: Concentrations, Speciation, Cycling, And Isotopic Composition, Garry Brown

Electronic Theses and Dissertations

The goal of this project is to better understand the biogeochemical cycle of mercury (Hg) in Mississippi to aid those managing the resources and developing related policy. To that end, Hg levels were determined in a total of 202 fish from Enid, Sardis and Grenada Lakes; Enid and Grenada have fish consumption advisories due to Hg. In addition, total-Hg and methylmercury (MeHg) levels were determined in the inflow and outflows from Enid and Sardis Lakes and in bottom sediment and suspended solids in Enid Lake. Potential sources of Hg to Enid Lake were investigated, including precipitation and runoff from urban, …

Screening Of Quantum Dots For Toxicity On The Growth And Viability Of Escherichia Coli, Jeremy Tharkur

Electronic Theses and Dissertations

Heavy metal (HM) containing quantum dots (Qdots) are increasingly used in commercial products due to their unique electronic, optoelectronic, optical and magnetic properties. Once disposed to the landfill, environmental weathering is likely to compromise HM Qdot integrity, leading to release of heavy metal ions. To minimize any negative environmental impact of HM Qdots, there is an increasing demand for developing HM free or environmentally-friendly surface modified HM Qdot alternatives. In this study, synthesis of HM free ZnS:Mn/ZnS and surface modified HM CdS:Mn/ZnS Qdots (using N-acetylcysteine, NAC, and Dihydrolipoic acid, DHLA) and their potential toxicity assessment using E. coli as a …

Silicon Photonic Devices For Optical Delay Lines And Mid Infrared Applications, Saeed Khan

Electronic Theses and Dissertations

Silicon photonics has been a rapidly growing subfield of integrated optics and optoelectronic in the last decade and is currently considered a mature technology. The main thrust behind the growth is its compatibility with the mature and low-cost microelectronic integrated circuits fabrication process. In recent years, several active and passive photonic devices and circuits have been demonstrated on silicon. Optical delay lines are among important silicon photonic devices, which are essential for a variety of photonic system applications including optical beam-forming for controlling phased-array antennas, optical communication and networking systems and optical coherence tomography. In this thesis, several types of …

Is Breakdown Of Fatty Acid Peroxides Involved In The Induction Of Apolipoprotein A1?, Rajat Gupta

Electronic Theses and Dissertations

Over the past few years the number of deaths caused due to cardiovascular diseases has been increasing and is of major concern. In the United States, 75% of cardiovascular-related deaths have been attributed to atherosclerosis. Western diets containing large quantities of peroxidized lipids are considered atherogenic. Heated oil in the form of fried food brings high levels of peroxidized fat and its decomposition products in the diet. Peroxidized lipids are known to increase the susceptibility of serum lipoproteins to undergo oxidation, thereby contributing to the progression of atherosclerosis. The intestinal cells are responsible for the absorption of dietary fatty acid …

Identification Of Epithelial Stromal Interaction 1 And Epidermal Growth Factor Receptor As Novel Kruppel-Like Factor 8 Targets In Promoting Breast Cancer Progression, Tianshu Li

Electronic Theses and Dissertations

Breast cancer is the major cause of cancer death among women worldwide. Understanding the mechanisms underlying breast cancer progression remains urgent for developing effective treatment strategies to eliminate breast cancer mortality. Our recent studies have demonstrated that Krüppel-like transcriptional factor 8 (KLF8) plays a critical role for breast cancer progression. Other studies have shown that Epithelial stromal interaction 1 (EPSTI1), a recently identified stromal fibroblast-induced gene in non-invasive breast cancer cells and epidermal growth factor receptor (EGFR) are highly overexpressed in aggressively invasive breast carcinomas including triple negative breast cancers. In this thesis project, we demonstrate high co-overexpression of KLF8 …

Preliminary Validation Of Handheld X-Ray Fluorescence (Hhxrf) Spectrometry: Distinguishing Osseous And Dental Tissue From Non-Bone Material Of Similar Chemical Composition, Heather Zimmerman

Electronic Theses and Dissertations

Forensic anthropologists normally examine bone from a variety of medicolegal contexts. The skeletal remains may in some cases be highly fragmented or taphonomically modified, making it difficult to sort bone from non-bone material. In these cases, the forensic anthropologist may rely on microscopic or destructive chemical analyses to sort the material. However, these techniques are costly and time-intensive, prompting the use of nondestructive analytical methods in distinguishing bone and teeth from non-bone materials in a limited number of cases. The proposed analytical techniques are limited in that they rely on an examination of the major elements in the material, and …

Influence Of Task-Role Mental Models On Human Interpretation Of Robot Motion Behavior, Scott Ososky

Electronic Theses and Dissertations

The transition in robotics from tools to teammates has begun. However, the benefit autonomous robots provide will be diminished if human teammates misinterpret robot behaviors. Applying mental model theory as the organizing framework for human understanding of robots, the current empirical study examined the influence of task-role mental models of robots on the interpretation of robot motion behaviors, and the resulting impact on subjective ratings of robots. Observers (N = 120) were exposed to robot behaviors that were either congruent or incongruent with their task-role mental model, by experimental manipulation of preparatory robot task-role information to influence mental models (i.e., …

Systems Geometry: A Methodology For Analyzing Emergent System Of Systems Behaviors, Christina Bouwens

Electronic Theses and Dissertations

Recent advancements in technology have led to the increased use of integrated ‘systems of systems’ (SoS) which link together independently developed and usable capabilities into an integrated system that exhibits new, emergent capabilities. However, the resulting SoS is often not well understood, where secondary and tertiary effects of tying systems together are often unpredictable and present severe consequences. The complexities of the composed system stem not only from system integration, but from a broad range of areas such as the competing objectives of different constituent system stakeholders, mismatched requirements from multiple process models, and architectures and interface approaches that are …

Transport And Metabolism Of Glycerophosphodiesters By Candida Albicans, Andrew C Bishop

Electronic Theses and Dissertations

Glycerophosphodiesters are products of phospholipase B-mediated hydrolysis of phospholipids. Their transport and metabolism is elaborated in the fungal pathogen, Candida albicans, as compared to the non-pathogenic Saccharomyces cerevisiae. C. albicans contains four ORFs (CaGIT1-4) predicted to encode transporters for glycerophosphodiesters, compared to one (ScGIT1) in S. cerevisiae. Here I have identified the gene products responsible for glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) transport. C. albicans strain lacking ORF 19.34, which codes for CaGit1, is unable to transport intact GroPIns. Transport activity can be rescued by reintegration of one copy of CaGIT1 back into the genome. Similarly, a strain lacking CaGIT3 (ORF …

Synthesis Of Substituted Pyrrolo[2,3-D]Pyrimidines As Microtubule-Binding Agents And Hsp90 Inhibitors, Lu Lin

Electronic Theses and Dissertations

An introduction, background and recent advances in the areas of microtubule-binding agents and heat shock protein 90 (HSP90) inhibitors as anticancer agents are briefly reviewed. The work in this dissertation is centered on the synthesis of substituted pyrrolo[2, 3-d]pyrimidines as potential anticancer agents that act via microtubule inhibition or HSP90 inhibition..

Microtubule-binding agents are effective against a broad range of tumors and lymphomas and have been common components of combination cancer-chemotherapy in the clinic. Despite the unparalleled success, drawbacks among microtubule-binding agents such as multi-drug resistance, dose-limiting toxicity, poor pharmacokinetic profile and high cost have supported the sustaining momentum in …

Medication Cost And Utilization In Hospice Care: An Analysis Of 2007 Claims Data, Rachi Parekh

Electronic Theses and Dissertations

Objectives:

1. Describe patient-related and hospice-related characteristics such as gender, average length of hospice stay, primary diagnoses, average daily census, number of admissions per year, net operating revenues, inpatient unit (IPU) operating costs, and medication expenditures of hospices in Ohio and compare it with NHPCO data.

2. Identify and analyze therapeutic drug classes and medications with the most frequent utilization rates and largest percentage of expenditures in hospice care.

Method:

Hospice Pharmacy Benefit Managers (PBM) employ pharmacists to provide comprehensive pharmaceutical care services for patients under hospice care and one component of pharmacist provided services include comprehensive medication review. To …

"Individual Yet As One": Performing Deafness And Performing Community In Mark Medoff's Children Of A Lesser God, Mariah Crilley

Electronic Theses and Dissertations

In this thesis, I examine the relationship between deafness, women, and performance in Mark Medoff's Children of a Lesser God. The play was a massive popular success, both in its run on Broadway and its movie adaptation. Deafness and deaf people had never been so visible in American hearing culture. More importantly, the play coincided with civil rights movements by people with disabilities, which culminated in the passage of the Americans with Disabilities Act. Disabilities, including deafness, were called into being as part of a national identity. These movements posited self-determination but ultimately relied and thrived on a communal and …

Molecular Evolution Of Hominoid Seminal Plasma Genes, Sarah Jean Carnahan Craig

Electronic Theses and Dissertations

When asking the question of what underlying genetic changes drive species to be different, it is debated whether protein coding or non-coding mutations are more important. The hominoids (humans, great apes, and gibbons) are a group of closely related species, yet vary widely in their mating system behaviors and predicted levels of sperm competition. In species where females mate with multiple males, sperm competition is hypothesized to increase the rate of adaptive evolution of proteins expressed in male reproductive tissues through recurrent selective sweeps (positive selection). Previous studies have shown this to be true for individual male reproductive genes. In …

Computational Methods For Comparative Non-Coding Rna Analysis: From Structural Motif Identification To Genome-Wide Functional Classification, Cuncong Zhong

Electronic Theses and Dissertations

Recent advances in biological research point out that many ribonucleic acids (RNAs) are transcribed from the genome to perform a variety of cellular functions, rather than merely acting as information carriers for protein synthesis. These RNAs are usually referred to as the non-coding RNAs (ncRNAs). The versatile regulation mechanisms and functionalities of the ncRNAs contribute to the amazing complexity of the biological system. The ncRNAs perform their biological functions by folding into specific structures. In this case, the comparative study of the ncRNA structures is key to the inference of their molecular and cellular functions. We are especially interested in …