Digital Commons Network^™

Effect Of Six-Day Germination On Chemical Composition, Functional, And Nutritional Properties Of Pea Varieties, Hojjat Abdollahikhamene

Electronic Theses and Dissertations

Pulses, particularly peas, have long been a crucial source of protein in vegetarian and cost-effective diets worldwide. They have gained popularity in Western diets as more people turn to plant-based proteins as alternatives to animal products. Peas are known for their high protein content; however, their use in food products has been limited due to factors like poor solubility and digestibility. Germination improves the nutritional profile of peas by enhancing protein content and digestibility. The objective of this study was to determine the effects of six days of germination on the nutritional composition and functional properties of different varieties of …

Biopolymer-Induced Soil Ductility Enhancement: Mitigating The Effects Of Desiccation Cracks, Rabindra Prasad Bohara

Electronic Theses and Dissertations

This study addresses the formidable challenges of expansive clayey soils, notorious for their swelling-shrinkage characteristics, which often lead to ground instability and structural damage. While effective in mitigating soil swell-shrink potential and enhancing strength, traditional calcium-based stabilizers can react with higher sulfate content to form ettringite, causing volumetric changes and infrastructure distress. To assess the impact of stabilizing sulfate-rich soils and determining the various engineering properties, samples of both control and stabilized soils were prepared using lime, biopolymer (guar gum), and lime with guar gum at concentrations of 2% and 4% lime only, 0.5%, 1% and 1.5% guar gum only, …

Evaluation Of Strip-Tillage And Banded Phosphorus And Potassium Fertilizer For Corn Production In Eastern South Dakota, Charles N. Tesch

Electronic Theses and Dissertations

In South Dakota, the adoption of no-till and other conservation tillage practices has grown in recent years. However, conventional tillage is still a common practice in the eastern third of the state, negatively affecting soil health and the environment. Strip-tillage has gained interest in this region as a way to transition to conservation tillage while retaining some benefits of conventional tillage. Strip-tillage also provides the ability to subsurface band nutrients, particularly phosphorus (P) and potassium (K) fertilizers within the tillage strip, potentially improving nutrient accessibility and recovery while reducing environmental risks. This study aimed to investigate (i) the effects of …

Investigating The Ruminal Metagenome Of Grass And Forage-Fed Bison To Uncover Metabolic Activities That Impact The Efficiency Of Plant Fiber Utilization, Anlly Fresno Rueda

Electronic Theses and Dissertations

This dissertation investigates the ruminal microbiome of American plains bison (Bison bison) to uncover the metabolic activities that enable efficient utilization of plant fiber, with a particular focus on bacterial composition and functional capabilities in response to forage quality. Using both 16S rRNA gene sequencing and metagenomic approaches, this research provides a comprehensive view of microbial diversity and metabolic potential in the rumen of bison, offering insights into their ecological resilience and efficiency in nutrient extraction. Chapter 2 focuses on the metabolic potential of bacterial communities in the rumen of bison fed a grass-based diet. Using high-throughput sequencing and metagenomic …

Identification Of Gut Bacterial Symbionts From Atlantic Salmon That Are Involved In Metabolizing Feed Ingredients Derived From Soybean Meal, Emily Fowler

Electronic Theses and Dissertations

In the aquaculture industry, plant-based protein sources have emerged as an economically and environmentally sustainable replacement for traditional animal protein sources. Yet, due to reduced digestibility, the presence of anti-nutritional factors, as well as an increased risk of inflammation of the digestive system, the inclusion of plant-based protein ingredients must be limited, especially in the diets of carnivorous fishes. To gain further insight on how to adapt the fish digestive tract to more effectively utilize or tolerate plant-based protein ingredients, slurry from cultured Atlantic Salmon (Salmo salar) was used as a proxy in an in vitro model system to identify …

Development Of A Collagen-Based Bioink For 3d Printing Biomimetic Tendon-To-Bone Tissue Grafts For Rotator Cuff Repair, Samiul Nibir

Electronic Theses and Dissertations

This study presents the development of a collagen-based bioink for 3D printing tendon-to-bone tissue grafts, specifically designed for rotator cuff repair applications. Three different bioink formulations were developed: (i) 5.1% (w/v) collagen I, (ii) 5% (w/v) collagen I and collagen II, and (iii) 4.5% (w/v) collagen I combined with β-TCP. The bioink containing collagen I and collagen II was prepared by mixing the two components in a 1:1 ratio, while the collagen I plus β-TCP formulation was made by incorporating 20 wt % β-TCP into the collagen I solution. These formulations were developed to replicate the natural tendon-to-bone complex interface …

Exploring The Role Of Clinical And Social Factors In Predicting All-Cause Mortality In End-Stage Kidney Disease Patients: A Machine Learning Framework, Addy Smith

Electronic Theses and Dissertations

No abstract provided.

Mechanisms Leading To Cell Survival Through Membrane Repair In Pyroptosis, Prafulla Shrestha

Electronic Theses and Dissertations

No abstract provided.

Modeling Fluid Flow In Complex Biological Systems: Implications For Dense Tumor Microenvironments, Metastasis, And Bone Cancer, Mohammad Mehedi Hasan Akash

Electronic Theses and Dissertations

The transport of biofluids in physiological systems plays a critical role in understanding and optimizing therapeutic interventions for diseases such as cancer and respiratory infections. This dissertation focuses on developing and validating a comprehensive computational framework for studying multiphase transport in complex biological environments. The research emphasizes the use of Computational Fluid Dynamics (CFD) to simulate and analyze plasma perfusion in solid tumors, particle deposition in respiratory airways, and fluid transport in cancer metastasis and bone cancer systems. By addressing the challenges of modeling biological transport phenomena, this work provides valuable insights into therapeutic planning and experimental validation. The study …

Shifting Waters: A 41-Year Time-Series Analysis Of Surface Water Change In The Prairie Pothole Region, Madison Dejarlais

Electronic Theses and Dissertations

The Prairie Pothole Region (PRR) is a landscape defined by a dynamic surface water system, with wetlands, lakes, and rivers playing critical roles in supporting biodiversity, regulating floods, and maintaining hydrological balance. This study conducts a time-series analysis of surface water change in the PPR from 1984 to 2024, using remote sensing tools including Google Earth Engine, ArcGIS Pro, and ENVI. 41 annual surface water presence rasters were compiled into a cumulative dataset, allowing for both year-to-year comparisons and broader trend analysis. Results indicate that the presence of surface water in the PPR has not followed a simple linear trend. …

Synchronization Protocols Utilizing Progesterone Implants Associated With Estrus Expression, Vaginal Microbiome, And Immune Response In Beef Cattle, Riley James Puck

Electronic Theses and Dissertations

This thesis aims to provide a deep understanding of presynchronization strategies and the varying factors affecting their efficacy. Beginning with a literature review of the estrous cycle and the endocrinology driving it. Building on this foundation, the physiological basis for estrous synchronization is described, followed by the advancement of pre-synchronization protocols designed to increase estrus and conception. Further, we will focus on the influence of estrus expression on insemination time and conception rates. Finally, how the immune system interacts with the conception process. In addition, the review goes beyond exogenous endocrine manipulation and dives into the emerging role of the …

One Rung At A Time: Climbing The Ladder Toward Aquatic Connectivity In Eastern South Dakota, Evan Spencer

Electronic Theses and Dissertations

Roads and streams are synonymous with their connection to movement, the acquisition of resources, and as pathways to new opportunities. However, the factors that define good roads and ecologically connected streams are rarely without conflict. Road infrastructure at stream-road crossings has the potential to fragment stream networks. Tube culverts, where streams pass under the road through metal pipes, are particularly concerning for stream connectivity. Undersized, aging, or inappropriately installed culverts can develop vertical drops at the outflow due to high velocities and stream bed scouring. The outlet drop can function as a barrier, preventing the upstream movement of fish. Due …

Determining The Susceptibility Status Of Red Sunflower Seed Weevil, Smicronyx Fulvus (Coleoptera: Curculionidae) To Pyrethroid Insecticides, Aaron Lee Hargens

Electronic Theses and Dissertations

Although sunflowers are native to North America, the early breeding efforts to improve sunflowers occurred outside of the continent. These hybrids were reintroduced to North America around 1875. In the early 1900’s, efforts to increase oil content were achieved by selective breeding of the reintroduced hybrids. Although this resulted in the development of improved sunflowers, many of the traits that conferred resistance to native insect pests were lost. One insect pest, the red sunflower seed weevil was noted as having caused sunflower production to rapidly decline and eventually end in Illinois and Missouri. Unfortunately, red sunflower seed weevils are still …

Semantic Think-On-Graph (Semtog) : Enhancing Graphrag Through Semantic Community Detection, Sugam Mishra

Electronic Theses and Dissertations

Graph-based Retrieval-Augmented Generation (GraphRAG) enhances large language models (LLMs) by grounding their reasoning in structured knowledge graphs, making them more reliable for multi-hop reasoning and factual QA. A central mechanism in Think-on-Graph systems such as ToG[14] and FastToG[7] is community detection, which groups locally related nodes into compact subgraphs so that LLMs can reason over focused, information-rich neighborhoods instead of traversing the entire graph. However, these methods rely purely on structural connectivity, often scattering semantically related entities across different communities and weakening the evidence provided to the LLM. We propose Semantic Think-on-Graph (SemToG), a semantic-aware extension of FastToG[7] that integrates …

Multi-Source Remote Sensing–Based Soil Moisture Prediction Using Machine Learning, Niraj Neupane

Electronic Theses and Dissertations

Soil moisture (SM) plays a central role in climatic and environmental processes, influencing shear strength of soil, agricultural productivity, land–atmosphere interactions, and hydrologic functioning. However, accurately estimating SM across diverse climatic regions remains challenging due to spatial heterogeneity, limited in situ measurements, and inconsistencies in sensor resolution. Machine learning (ML) and remote sensing offer promising avenues for improving SM prediction, yet many existing approaches struggle with generalization across climatic gradients and often fail to capture temporal variability. This study integrates multi-source satellite and climate datasets, including SMAP L4_SM, MODIS land surface temperature, Daymet meteorological variables, and in situ observations from …

Quantification Of Auxin-Cytokinin Response During Lateral Root And Nodule Development In Medicago, Aniket Singh

Electronic Theses and Dissertations

Lateral roots (LRs) and nodules are post-embryonic root organs in legumes formed through the activity of de novo meristem. LRs, which are present in all vascular plants, are developed primarily via intrinsic developmental cues, whereas root nodules, found only in a small group of plants known as legumes, are formed in response to legume–rhizobium symbiosis. LRs arise from the pericycle layer of the root, whereas nodule initiation can begin either in the pericycle (indeterminate nodules with persistent meristem, as in Medicago) or in the cortex (determinate nodules with transient meristem, as in soybean), pointing toward a conserved developmental origin, especially …

Evaluating And Mapping Habitat Suitability And Effects Of Woodland Treatment On Breeding Pinyon Jays In Colorado, Emily Diane Macklin

Electronic Theses and Dissertations

The pinyon jay (Gymnorhinus cyanocephalus) is a highly social, colonially nesting corvid endemic to the interior western United States whose populations have declined by ~72% since 1966, prompting the species’ current review for listing under the Endangered Species Act. Pinyon jays inhabit pinyon pine (Pinus subsect. Cembroides) and juniper (Juniperus spp.) woodlands and other dry conifer woodlands, portions of which are treated through tree thinning and removal for fuels reduction, ecological restoration, and habitat improvement for wildlife. As a result there is pressing need to better understand how woodland treatment and other potential drivers impact pinyon jay habitat suitability and …

Fungal Biotransformation Of Thin Stillage And Soybean Hulls And Bacterial Production Of Lysine In Ethanol Industry Co-Products, Bhawana Khadka

Electronic Theses and Dissertations

Thin stillage (TS) and whole stillage (WS) are major by-products of the corn ethanol industry. These by-products are generated in large quantities and contain nutrients that remain after yeast fermentation. Current industrial utilization of these by-products involves a series of downstream processing steps such as centrifugation and evaporation, which are energy-intensive and often result in nutrient losses. Traditionally, these by-products are dried to produce distillers dried grains with solubles. In this study, microbial fermentation was investigated as an alternative bioprocessing tool to expand the utilization of these by-products. The first objective was to explore the upcycling of TS, both alone …

Characterization Of Major Foliar Fungal Species Affecting Cucurbits In South Dakota, Janani Perera Waduwarage Dona

Electronic Theses and Dissertations

Cucurbit crops comprise the largest area in vegetable production in South Dakota (USDA 2022). Information on the prevalence, distribution, and fungicide sensitivity of their fungal pathogens are unknown. This study characterized the major foliar fungal species affecting cucurbits in South Dakota through a 2-year, state-wide survey. Between 2023 and 2024, 789 leaves were recovered from pumpkin, cucumber, watermelon, squash, and bottle gourd. Alternaria spp. (70.72% of leaves) Fusarium spp. (6.84%), Podosphaera xanthii (4.18%), both Alternaria spp. and Fusarium spp. (3.42%), and both Alternaria spp. and Podosphaera xanthii (1.90%), Stagonosporopsis spp. (1.27%) and other genera of fungi (4.94%) were identified using …

Evaluation Of Soil Microbiome And Edaphic Factors In Response To Nitrogen Fertilizer Application In Western South Dakota Croplands, Riya Jain

Electronic Theses and Dissertations

No abstract provided.

Spatiotemporal Habitat Use, Sex-Specific Movement, And Site Fidelity Of Walleye In Lake Sharpe, Sd, Carter S. Dollen

Electronic Theses and Dissertations

Walleye Sander vitreus are a popular sportfish among anglers in South Dakota with large economic impacts to local communities. A popular destination for Walleye anglers is Lake Sharpe, a 25,000-ha reservoir on the Missouri River, in central South Dakota. Gill net catches in Lake Sharpe show high annual variation, prompting concern that gill net catch rates may be poorly related to population abundance. A better understanding of Walleye movement and distribution could lead to increased precision in annual abundance estimates and improved active management that increases angler success. The goals of this study were: 1) determine spatiotemporal movement patterns of …

Quantifying Avian And Vegetation Communities On Agricultural Conservation Easement Wetlands In Eastern South Dakota, Katherine Millman

Electronic Theses and Dissertations

The Prairie Pothole Region of eastern South Dakota contains numerous small depressional wetlands, colloquially known as potholes, that provide important ecosystem services, including water storage, water quality improvement, and wildlife habitat. The Agricultural Conservation Easement Program (ACEP) was established by the U.S. Department of Agriculture to protect and restore wetlands and grasslands on private land through easements such as Wetland Reserve Easements. Although ACEP requires management practices intended to maintain or improve habitat quality, few studies have evaluated their effectiveness at supporting wildlife communities and vegetation integrity. To address this gap, we evaluated wetlands at 30 ACEP sites and 20 …

Enhancing Alfalfa Persistence In Rangelands: Evaluating Morphological Traits Of Medicago Sativa Subsp. Falcata Germplasm, Abdulallah Mohammed Alomair

Electronic Theses and Dissertations

Rangelands cover nearly half of the Earth’s surface, representing about 54% (79 million km2), and occur in different types and climates worldwide. Alfalfa (Medicago sativa L.) is a major component in rangelands and covers about (320 thousand km2), representing about 0.4% of total global rangeland area. As a critical perennial legume crop, alfalfa can improve soil health by fixing approximately 336 kg/ha/year of nitrogen and sequestering up to 614 kg/ha/year of carbon, while enhancing livestock production due to its high content level of vitamins, magnesium, minerals, fiber, and protein. However, environmental stresses such as drought, freezing, and grazing limit alfalfa …

Extended Days On Feed: Influences On Growth Performance, Efficiency, And Carcass Characteristics Of Steers And Heifers Of Different Proportions Of Angus And Limousin Genetics, Grace Henley Olinger

Electronic Theses and Dissertations

Recently, cattle feeders have extended days on feed (DOF) resulting in hot carcass weights (HCW) exceeding the limits of existing serial slaughter datasets. This study's goal was to determine the impact extended DOF exerts on growth performance, feed efficiency, and carcass characteristics of steers and heifers with varying proportions of Angus (AN) and Limousin (LM) genetics. In 2022, commercial AN cows from two MT ranches were artificially inseminated to 9 sires from 3 breeds (AN, LM, and Limflex (LF)) with parentage confirmed by DNA. In 2023, 216 calves (sired by 72 AN, 73 LF, and 71 LM; 105 steers and …

Formulation And Immunogenicity Of L1 Orf1 Mrna Vaccine In Mice, Raj Nandani

Electronic Theses and Dissertations

This dissertation presents the design, formulation, and immunogenicity evaluation of a novel mRNA vaccine targeting the L1 ORF1p protein, a tumor-associated antigen. The vaccine was synthesized using in vitro transcription and encapsulated in lipid nanoparticles (LNPs), with both unmodified uridine and N1-methylpseudouridine variants tested. Physicochemical characterization confirmed optimal particle size (160–220 nm), acceptable zeta potential, and encapsulation efficiency ranging from 74% to 92%, with preserved RNA integrity post-formulation. These results validate the robustness of the formulation workflow and its suitability for antigen delivery. Immunogenicity studies in C57BL/6 mice revealed modest but promising immune responses. While early activation markers (CD69) and …

Effect Of Seed Orientation And Starter Fertilizer Placement In Corn Development, Indu Acharya

Electronic Theses and Dissertations

The orientation of corn (Zea mays L.) seeds during planting and the strategic placement of starter fertilizers represents two management practices that could potentially influence crop performance. Seed orientation may affect emergence, leaf architecture and light interception patterns, while starter fertilizers aim to enhance early season nutrient availability and seedling vigor. This research explored how these practices, independently and in combination, might shape corn development from emergence through harvest. We conducted field studies in 2022 and 2023 to investigate the impact of seed orientation, starter fertilizer placement and their interaction on corn seedling emergence, crop development, leaf direction and grain …

Exploring A Modern Deep Learning Technique For Wetland Mapping And Monitoring Using Worldview-2 Satellite Products, Mizanur Rahman

Electronic Theses and Dissertations

Wetlands play a significant role in the world’s hydrology, climate, and biodiversity. Even with the benefits and values wetlands provide to the environment, they have been undergoing loss and degradation due to natural and anthropogenic processes. To protect wetlands from loss and degradation and to restore their function, it is essential to develop a sustainable wetland monitoring system. One of the key elements of a wetland monitoring system is wetland mapping. This dissertation research developed an object-based deep learning protocol for mapping heterogeneous wetlands with many communities from a high-resolution WV-2 satellite image. To test this developed protocol, an object-based …

Motherhood: An Orphan Of American Politics A Comparative Analysis Of State Actors As Institutions, Kristina Ramskyte-Juszczak

Electronic Theses and Dissertations

The political geography of America is relatively unique in the world: the United States is a powerful and wealthy country, but also consists of sub-national actors that retain significant legal powers within their own borders. The political actors in America, at both this smaller level and at the larger Federal level, have uniquely strong institutional power to shape the social and civic life of their citizens. Despite their absolute significance to the survival of their societies, mothers and mothers’ rights have largely been ignored or taken second place to other political issues. As many countries in Europe and other regions …

Mblac1 Knockout Mice: Behavioral Modeling Of A Novel Risk Factor In Alzheimer's Disease, Cara Melillo

Electronic Theses and Dissertations

Alzheimer’s disease (AD) involves progressive cognitive decline, with mitochondrial dysfunction, oxidative stress, and copper (Cu) imbalance implicated in its pathogenesis. MBLAC1 has been identified as a risk factor for AD in individuals with comorbid cardiovascular disease (AD-CVD). Its C. elegans ortholog, swip-10, regulates Cu ion homeostasis and mitochondrial function. To investigate MBLAC1’s role, we examined behavioral phenotypes in Mblac1 knockout (KO) mice and tested the efficacy of the Cu chaperone elesclomol (ES). Male KO mice exhibited deficits in novel object recognition (NOR), which were rescued by ES treatment. Female KOs showed increased anxiety-like behavior in the Elevated Plus …

Applications Of Deep Learning And Signal Processing In Acoustics, Oceanography, And Biometrics, Ali Abdulmajeed Altaher

Electronic Theses and Dissertations

This dissertation examines multidisciplinary methods in marine research, computational modeling, and artificial intelligence. This dissertation tackles significant issues in ecological monitoring, ocean dynamics forecasting, and assistive technology via three independent but interrelated research streams.

The first study examines the localization and distribution of marine organisms along Florida’s East Coast via passive acoustic monitoring methods. The research analyzed more than 65,000 audio recordings to delineate the geographical distribution of several marine creatures, such as black drum, toadfish, Sei whales, North Atlantic right whales, dolphins, and false killer whales. A novel automated detection and localization model was developed, using an adaptive matching …