Digital Commons Network^™

Species Preference Of Viral Deubiquitinating Proteases Toward Isg15 Through Structural And Enzymatic Characterization, Michelle Kay Deaton

Electronic Theses and Dissertations

Proteases from the Ovarian Tumor domain (OTU) superfamily of deubiquitinating enzymes (DUBs) are expressed by a range of RNA viruses. Viral OTUs (vOTUs) are found in nairoviruses such as Crimean Congo Hemorrhagic Fever virus (CCHFV), Nairobi sheep disease virus (NSDV), the Erve virus (ERVEV), and the Dugbe virus (DUGV), as well as in the areterivirus Porcine Reproductive and Respiratory Syndrome virus (PRRSV), among others. vOTUs, which interfere with host innate immune response through editing of host Ub and Ub-like molecules such as interferon stimulated gene 15 (ISG15), have been identified as a potential virulence factor through their role in evading …

Risk For Engagement In Nonsuicidal Self-Injury In Children And Adolescents, Andrea Lee Barrocas Gottlieb

Electronic Theses and Dissertations

Although NSSI engagement is a growing public health concern, little research has documented the developmental precursors to NSSI in longitudinal studies using youth samples. This study aimed to expand upon previous research on groups of NSSI engagement in a population-based sample of youth using multi-wave data. Moreover, this study examined whether chronic peer and romantic stress, the serotonin transporter gene (5-HTTLPR), parenting behaviors, and negative attributional style predicted the NSSI group membership as well as the role of sex and grade. Participants were 549 youth in beginning in the 3^rd, 6^th, and 9^th grades at …

Finding Onesimus: Recovering The Story Of A First-Century Fugitive Slave, Ryan Lokkesmoe

Electronic Theses and Dissertations

This dissertation is an investigation into the experience of a first-century fugitive slave named Onesimus, who is known to us primarily through Paul’s letter to Philemon (Phlm) in the New Testament. Within this broader purpose, this project challenges a popular historical theory for Onesimus’ flight, the so-called Amicus Domini theory. This is the theory that Onesimus fled his master Philemon with the premeditated intention of seeking out the Apostle Paul as a peacemaker in a conflict Onesimus was having with Philemon. The Amicus Domini theory is accepted by many scholars, though rarely discussed in detail or examined critically.

The goal …

A Mixed Methods Evaluation Of The Effectiveness Of A Group Yoga Intervention As An Adjunctive Trauma Therapy For Adolescent Girls, Melissa Eileen Houser

Electronic Theses and Dissertations

Neuroscience findings support the need for trauma treatments that work from the lowest levels of the brain up to the highest levels of the brain (Perry, 2009) due to evidence that the inverse relationship between amygdala and medial prefrontal cortex is not as strong in individuals who have experienced trauma, leading to difficulty in inhibiting fear responses through cognition (McRae, Ochsner, & Gross 2011). Difficulties associating language with traumatic events have also been found (van der Kolk, 2006). The integration of mindfulness practices and the popularization of yoga in the West have led to use of yoga to address trauma …

Modern Capitalism And Food Commoditization: The Limitations Of Industrial Agriculture And The Challenges Of Sustainable Alternatives, Gerardo Patron-Cano

Electronic Theses and Dissertations

Agriculture is an essential function of contemporary human life that is bound by nature. Therefore, economic, social, and environmental perspectives must be examined to identify the most sustainable agricultural systems. This thesis argues that agriculture should be divorced from capitalist economic principles regarding specialization, trade, and production scale. Such principles have supported industrialized growing methods, which have been economically, socially, and environmentally unsustainable. In order for agriculture to be sustainable and equitable, food systems need to be de-commoditized and removed from the capitalist market. Policies should target the local control of food systems by empowering communities to subsidize localized production-consumption …

New Methods For Measuring Transient Interactions Between Proteins And Curved Membranes, Philip P. Cheney

Electronic Theses and Dissertations

Variations in the physical deformation of the plasma membrane play a significant role in the sorting and behavior of the proteins that occupy it. Determining the interplay between membrane curvature and protein behavior required the development and thorough characterization of a model plasma membrane with well defined and localized regions of curvature. This model system consists of a fluid lipid bilayer that is supported by a dye-loaded polystyrene nanoparticle patterned glass substrate. As the physical deformation of the supported lipid bilayer is essential to our understanding of the behavior of the protein occupying the bilayer, extensive characterization of the structure …

Phytoremediation Of Metal Contamination Using Salix (Willows), Gordon J. Kersten

Electronic Theses and Dissertations

Abandoned hardrock mines and the resulting Acid Mine Drainage (AMD) are a source of vast, environmental degradation that are toxic threats to plants, animals, and humans. Cadmium (Cd) and lead (Pb) are metal contaminants often found in AMD. In my mine outwash water samples, cadmium and lead concentrations were 19 and 160 times greater than concentrations in control waterways, and 300 and 40 times greater than EPA Aquatic Life Use water quality standards, respectively. I tested the phytoremediation characteristics of three montane willows native to the Rocky Mountains: Salix drummondiana, S. monticola, and S. planifolia. I tested …

The Effects Of Molecular Chaperones On Tau Fibril Assembly, Ahmed Omran

Electronic Theses and Dissertations

The accumulation of microtubule-associated protein tau into fibrillar aggregates is the hallmark of Alzheimer’s disease and other neurodegenerative disorders, collectively referred to as tauopathies. Fibrils can propagate from one cell to the next and spread throughout the brain. However, a study shows that only small aggregates can be taken up by cultured neuronal cells. The mechanisms that lead to the breakage of fibrils into smaller fragments remain unknown. In yeast, the AAA+ chaperone HSP104 processes the reactivation of protein aggregates and is responsible for fragmentation of fibrils. This study focused on investigating the effects of molecular chaperones on tau fibrils …

Examining The Relationship Among Genes, Attention Bias To Emotion, Depression In Youth, Jessica L. Jenness

Electronic Theses and Dissertations

The investigation of biologically initiated pathways to psychological disorder is critical to advance our understanding of mental illness. Research has suggested that attention bias to emotion may be an intermediate trait for depression associated with biologically plausible candidate genes, such as the serotonin transporter (5-HTTLPR) and catechol-o-methyl-transferase (COMT) genes, yet there have been mixed findings in regards to the precise direction of effects. The experience of recent stressful life events (SLEs) may be an important, yet currently unstudied, moderator of the relationship between genes and attention bias as SLEs have been associated with both gene expression and attention to emotion. …

Thermally Induced Motion, Collision And Mixing Of Levitated Droplets, Ashkan Davanlou

Electronic Theses and Dissertations

This dissertation investigates the motion of a levitated droplet experimentally and analytically against the Marangoni flow in an immiscible outer fluid at higher speeds than is possible currently. Based on our earlier experiments, when a droplet is released from a height of 1.5 – 4 times its diameter from the liquid surface, it can overcome the impact and stay levitated at the liquid-air interface due to the existence of an air gap between the droplet and the liquid film. In order to explain this behavior of droplet traveling against the counter-current motion, we propose a simple approach: first, the Marangoni …

Biomechanics Of Developmental Dysplasia Of The Hip - An Engineering Study Of Closed Reduction Utilizing The Pavlik Harness For A Range Of Subtle To Severe Dislocations In Infants., Victor Huayamave

Electronic Theses and Dissertations

Developmental Dysplasia of the Hip (DDH) is an abnormal condition where hip joint dislocation, misalignment, or instability is present in infants. Rates of incidence of DDH in newborn infants have been reported to vary between 1 and 20 per 1000 births, making it the most common congenital malformation of the musculoskeletal system. DDH early detection and treatment is critical to avoid the use of surgical treatment in infants and to prevent future complications such as osteoarthritis in adult life. To this day several non-surgical treatments involving the use of harnesses and braces have been proposed to treat DDH in infants, …

It's A Conspiracy: Motivated Reasoning And Conspiracy Ideation In The Rejection Of Climate Change, Nikilaus Wycha

Electronic Theses and Dissertations

A large disconnect exists between the general public's acceptance of human-caused climate change and the prevailing consensus of actively publishing scientists. Previous research has examined both political and economic motivated reasoning, media influence in print and television, conspiracy ideation as a predictor of science rejection, and the role of the social construction of scientific knowledge in science rejection. Using these previously studied justifications for climate change rejection as a starting point, this research examines 212 written responses to a prompt at Climate Etc. asking the community to explain their acceptance / rejection of climate change. Using a textual content analysis, …

Environmentalism And Environmental Constitutional Ballot Initiatives In Florida: The Elements Of Support For Amendment One In 2014 In The Context Of Current Environmental Attitudes., Michael Jones

Electronic Theses and Dissertations

Americans express support for “the environment” with environmental support cutting across political and demographic differences and cleavages. In the past 15 years, however, period effects, political sorting, and the emergence of a powerful anti-environmental movement have lessened the generalized levels of environmental support. Using the 2012 CCES survey, the expressed attitudes regarding multiple environmental issues found significant differences in levels of environmental support nationally by party, Tea Party attitudes, ideology, and certain demographic characteristics. For Floridians, the differences between the most pro-environmental respondents and the most anti-environmental are narrower; partisan identification itself is not significant in environmental attitudes; but ideology, …

Modeling Crowd Mobility And Communication In Wireless Networks, Gurkan Solmaz

Electronic Theses and Dissertations

This dissertation presents contributions to the fields of mobility modeling, wireless sensor networks (WSNs) with mobile sinks, and opportunistic communication in theme parks. The two main directions of our contributions are human mobility models and strategies for the mobile sink positioning and communication in wireless networks. The first direction of the dissertation is related to human mobility modeling. Modeling the movement of human subjects is important to improve the performance of wireless networks with human participants and the validation of such networks through simulations. The movements in areas such as theme parks follow specific patterns that are not taken into …

Quantitative Assessment Of The Effects Of Microbial Degradation Of A Simple Hydrocarbon Mixture, Jessica Kindell

Electronic Theses and Dissertations

Ignitable liquids consist of either a single organic compound or a complex organic mixture. In regards to fire debris analysis, the analyst is responsible for determining if an ignitable liquid residue is present. However, when extracted from soil-containing fire debris evidence, chemical degradation from microorganisms is observed to result in the loss of compounds based on chemical structure. It can also happen when the evidence container is stored at room temperature before analysis. This can present a challenge to the fire debris analyst when identifying and classifying the ignitable liquid residue based on the criteria established by standard test methods. …

Synthesis, Linear And Nonlinear Photophysical Characterization Of Two Symmetrical Pyrene-Terminated Squaraine Derivatives In Solution, Alfonso Ballestas Barrientos

Electronic Theses and Dissertations

Two indole-based squaraine dyes bonded to two pyrenyl groups through vinyl- and ethynyl- linkers were synthesized with the aim of enhancing the intramolecular charge transfer interaction in addition to improving their optical properties. The absorption and emission properties of these derivatives were determined in order to gain an insight into the intensity of this type of interaction, their aggregation behavior and compare them with results obtained through quantum chemical calculations. Both compounds presented high photochemical stability in THF, and the linear spectroscopic characterization revealed high extinction coefficients, large fluorescence quantum yields and relatively low tendency of forming excimers in several …

Sexism And Women: The Implications Of Female Gender Resentment, Christine Regnier-Bachand

Electronic Theses and Dissertations

Do women suffer from a societal Stockholm Syndrome which leads them to display high levels of modern and traditional sexist behavior? Does a woman*s level of modern or traditional sexist behavior influence her political choices? Female gender resentment and sexist biases are an understudied area of the extant literature on sexism. Typically the focus is placed on men*s sexist attitudes and treatment of women, but is it possible that women also contribute to the subordination of their gender through sexist practices? These are questions which this thesis attempts to answer. The findings indicate that working women under the age of …

Photogenerated O-Azaxylylenes: Mechanistic Studies And Synthetic Applications, William Cole Cronk

Electronic Theses and Dissertations

This research sets out to build upon excited state o-azaxylylene cycloaddition. The mechanism behind the excitation and cycloaddition process of photogenerated o-azaxylylenes was determined experimentally. Time-correlated single-photon counting, steady-state spectroscopy, triplet quenching experiments, and quantum yield studies provided evidence suggesting that excited state intramolecular proton transfer is followed by intersystem crossing and stepwise addition to the tethered unsaturated pendant.

In keeping with the principles of diversity oriented synthesis, a modular approach was taken to gain access to a diverse array of N,O,S-Polyheterocycles which were modified postphotochemically via Suzuki coupling to yield fused biaryls. Cycloaddition products, outfitted with halogens …

My Blood Of The (New) Covenant: An Assessment Of René Girard's Soteriology In Light Of The Covenantal Milieu Of The Last Supper Sayings, Benjamin Burkholder

Electronic Theses and Dissertations

This study assesses René Girard's claims regarding the Gospels' understanding of Jesus' death. Though Girard contends that the Gospels never depict Jesus' death as an atonement for sin, there are significant passages that Girard avoids discussing like the Last Supper sayings in the Synoptic Gospels. This dissertation investigates whether these central passages, along with other supporting texts in the Synoptics, jeopardize the viability of Girard's assertions, especially when they are read in light of restoration theology.

The core components of Girard's thought, his reading of salvation history, and the ways in which Girard's followers have adapted his thought are adumbrated …

Novel Solid-Phase Extraction Techniques For Biological And Environmental Analysis Using Isotope Dilution Mass Spectrometry, Andrew Boggess

Electronic Theses and Dissertations

Awareness and study of the ways in which the environment can interact with the personal genetics and epigenetics of an individual has grown substantially in recent years, resulting in the field of Exposomics. In an era of increasingly personalized medicine, novel techniques are necessary to ensure the accurate and sensitivity measurement of clinically and environmentally relevant molecules in biological and environmental samples. Addressing existing shortcomings cited in literature, methods were developed and optimized for the extraction, separation, mass analysis, and quantification of a suite of environmental organic pollutants in both biological and environmental samples, with the primary objective of improving …

Poverty And The Challenge Of Quality Primary And Secondary School Education Access In Mara-Tanzania, Agnes Bhoke-Africanus

Electronic Theses and Dissertations

Although poverty draws global deliberations, still it continues to be a complicated parable, driving people to the scarceness of opportunities, resources and capability deprivation as a result of education limitation–leading to ignorance decease, again poverty, and its consequences. As I write, education has been promoted in Tanzania, and more children have access to education than ever, and yet, it doesn't warrant that children have a fair access to quality education. The marginalized children, including children from poor families, orphans, children with disabilities, street children, and nomadic children hang on struggling to access quality education.

The goal of this study was …

From Their Own Voices: The Lived Experiences Of African Americans Exposed To Jim Crow, Janelle R. Carter

Electronic Theses and Dissertations

Researchers have become increasingly interested in the impact of racism on African Americans; however, because interactions involving racism are highly subjective, it can be difficult to assert the presence of trauma in a social science context. Thus, whereas literature regarding the effects of racism is abundant, few studies have explored the effects of being exposed to the laws of the "Jim Crow" era. This study sought to illuminate the lived experiences of African Americans who were exposed to the racial caste system known as Jim Crow. This inquiry looked at transgenerational trauma, exploring exposure to decades of legal discrimination, while …

Evolution Of Prostate Specific Gene Expression Associated With Post Copulatory Sexual Selection, Scott Hergenrother

Electronic Theses and Dissertations

Hominoid primate species differ remarkably in their social grouping and mating systems, notably including differing degrees of post-copulatory sexual selection. As the mating system of extinct hominins remains unknown and difficult to predict, it may be useful to examine more proximate phenotypes correlated with behavior. For example, chimpanzees and bonobos have a large ejaculate that coagulates into a rigid copulatory plug, presumably in response to high levels of sperm competition, while gorillas have a small semi-viscous ejaculate associated with low sperm competition. To understand the molecular basis responsible for differences in semen biochemistry among hominoid species, I completed two research …

Negative Effects Of Commycorrhizal Networks And Roots On Upland Oak Seedlings In Open-Canopy Woodlands And Closed-Canopy Forests, William Chase Bailey

Electronic Theses and Dissertations

After extensive logging and fire suppression many oak dominated woodlands or forests are in danger of being replaced by a mix of non-pyrogenic and shade tolerant tree species that benefit from fire suppression. Successful advanced regeneration by oaks in forests and woodlands depends both on the persistence of seedlings in the shade and growth within canopy gaps. Through the sharing of carbon and/or water between adults and seedlings, connection to a commycorrhizal network potentially provides a mechanism by which oak seedlings could persist in shade and/or grow rapidly in dry soils within canopy gaps. A study was conducted to determine …

Language Policy Implementers: Faculty Perspectives On Language Policy At A Southeastern U.S. University, Jordan Nicholas Troisi

Electronic Theses and Dissertations

Faculty members, as language policy implementers, retain significant control of the enactment of policy in local contexts. In order to better understand the relationship between de jure language policies at multiple levels of the policy hierarchy, in addition to faculty members? acceptance or rejection of these policies, this study investigated faculty perspectives on language policy, specifically at a southeastern university in the United States. This study employed multiple methods of research including a policy examination and a survey. These methods were selected in order to inspect de jure language policies at the national, state, governing board, and institutional level as …

Examination Of Accelerometer Reactivity Among Children, Adolescents, And Adults: Implications For Influencing Physical Activity Estimates, Proportion Meeting Physical Activity Guidelines, And Associations With Health Outcomes, Robert Eugene Davis

Electronic Theses and Dissertations

The health benefits of regular Physical activity (PA) have been rigorously documented and continue to be investigated from the standpoint of prevention, as well as, treatment of a multitude of adverse health outcomes including cardiovascular disease, obesity, sleep disorders, depressive symptomatology and countless others. In order to minimize subjectivity and more accurately measure PA behavior, researchers often use accelerometers. Although this method is considered valid and reliable a methodological issue that has rarely been addressed is the potential “reactivity” effect of measuring PA with an objective measure, such as accelerometry. Reactivity is a behavior change by the participant due to …

The Experience Of Physical And Social Presence In A Virtual Learning Environment As Impacted By The Affordance Of Movement Enabled By Motion Tracking, Aleshia Hayes

Electronic Theses and Dissertations

This research synthesizes existing research findings that social presence (sense of connection with others) and physical presence (sense of being there) increase learning outcomes in Virtual Learning Environments (VLEs) with findings that traditional motion tracking of participants wearing head mounted displays in virtual reality increases both physical and social presence. This information suggests that motion tracking in mixed reality VLEs has a positive impact on social presence and on physical presence. For this study, the affordance of free movement among virtual objects is enabled by Microsoft Kinect tracking of the user's position that is translated to movement of the virtual …

Synchrotron Based Infrared Microspectroscopy Of Carbonaceous Chondrites., Mehmet Yesiltas

Electronic Theses and Dissertations

Relationships between organic molecules and inorganic minerals are investigated in five carbonaceous chondrites, Northwest Africa 852 (CR2), Tagish Lake (C2-ungroupped), Orgueil (CI1), Sutter's Mill (CM), and Murchison (CM2), with micron spatial resolution using synchrotron-based imaging micro-FTIR spectroscopy. Correlations based on absorption strength for various constituents are determined using statistical correlation analysis. Silicate band is found to be positively correlated with stretching modes of aliphatic hydrocarbons in NWA 852 and Tagish Lake. The former is highly correlated with the hydration band in all meteorites. Negative correlation is observed between water+organics and carbonate bands in all meteorites. Two dimensional infrared maps for …

Evaluation Of Intestinal Microbial Diversity And A New Antibiotic Regimen In Crohn's Disease Patients, Karel Alcedo

Electronic Theses and Dissertations

Crohn's disease (CD) is a chronic granulomatous inflammatory bowel disease involving Mycobacterium avium subspecies paratuberculosis (MAP). Other microorganisms such as adherent-invasive Escherichia coli (AIEC) have also been proposed in CD association. To date, only one study investigated both MAP and AIEC simultaneously using peripheral blood but not in affected intestinal tissues. A standardized and effective antibiotic therapy against MAP and/or AIEC is needed for better treatment. Three antibiotic drugs – Clarithromycin (CLA), Rifabutin (RIF), and Clofazimine (CLO) have been used to treat CD patients suspected with MAP infection. However, the outcome has been controversial. The treatment dosage is high, the …

Genetically-Programmed Suicide Of Adrenergic Cells In The Mouse Leads To Severe Left Ventricular Dysfunction, Impaired Weight Gain, And Symptoms Of Neurological Dysfunction, Aaron Owji

Electronic Theses and Dissertations

Phenylethanolamine-N-methyltransferase (Pnmt) catalyzes the conversion of noradrenaline to adrenaline and is the last enzyme in the catecholamine biosynthetic pathway. Pnmt serves as a marker for adrenergic cells, and lineage-tracing experiments have identified the embryonic heart and hindbrain region as the first sites of Pnmt expression in the mouse. Pnmt expression in the heart occurs before the adrenal glands have formed and prior to sympathetic innervation, suggesting that the heart is the first site of catecholamine production in the mouse. The function of these Pnmt+ cells in heart development remains unclear. In the present study, we test the hypothesis that (i) …